Lag0027114.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0027114.1 vs. NCBI nr
Match: KAA0044057.1 (pathogenesis-related protein 1C-like [Cucumis melo var. makuwa])

HSP 1 Score: 274.2 bits (700), Expect = 7.7e-70
Identity = 129/187 (68.98%), Postives = 146/187 (78.07%), Query Frame = 0

Query: 1   MSPPCILSAVAAFS--LLLPAVSADQAPPPPPAAANQN-------PQRRGSLAREFLQAH 60
           MS P I SA  +FS  L+L    A   P  PP +A++N       P RR +  REFLQAH
Sbjct: 1   MSLPYITSAAFSFSFLLILHLAVAGVQPQSPPTSASKNSMTATTAPPRRRNYIREFLQAH 60

Query: 61  NKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGHWTPSAVV 120
           NK RS F + PLTWDKNLTR+A RWG+KRA DC+M HSYGPYGEN+FWGKL HWTP+ VV
Sbjct: 61  NKFRSAFHIQPLTWDKNLTRYAWRWGEKRAEDCRMIHSYGPYGENMFWGKLEHWTPTEVV 120

Query: 121 ESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVICEYDPPGN 179
           ESWAGE K+YNLN+NECA GQ CGHYTQIIW+E+LRLGCARVNC NGELLVICEYDPPGN
Sbjct: 121 ESWAGEEKYYNLNTNECADGQTCGHYTQIIWKESLRLGCARVNCDNGELLVICEYDPPGN 180

BLAST of Lag0027114.1 vs. NCBI nr
Match: KAE8651020.1 (hypothetical protein Csa_001683 [Cucumis sativus])

HSP 1 Score: 258.5 bits (659), Expect = 4.4e-65
Identity = 112/147 (76.19%), Postives = 126/147 (85.71%), Query Frame = 0

Query: 32  AANQNPQRRGSLAREFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYG 91
           A   +P+RR S  REFLQAHNK RS F + PLTWD+NLTRFARRWG++RAADC+M HSYG
Sbjct: 3   ATTASPRRR-SYIREFLQAHNKFRSAFHIQPLTWDRNLTRFARRWGEQRAADCRMIHSYG 62

Query: 92  PYGENLFWGKLGHWTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCA 151
           PYGEN+FWGKL HWTP+ VV SWAGE K YNL++NECA GQ CGHYTQIIW+E+LRLGC 
Sbjct: 63  PYGENMFWGKLEHWTPTEVVNSWAGEDKHYNLDTNECADGQTCGHYTQIIWKESLRLGCV 122

Query: 152 RVNCVNGELLVICEYDPPGNYVNEKPV 179
           RVNC NG LLVICEYDPPGNYVNEKP+
Sbjct: 123 RVNCDNGGLLVICEYDPPGNYVNEKPI 148

BLAST of Lag0027114.1 vs. NCBI nr
Match: XP_022938296.1 (pathogenesis-related protein 1C-like [Cucurbita moschata])

HSP 1 Score: 245.0 bits (624), Expect = 5.0e-61
Identity = 113/183 (61.75%), Postives = 136/183 (74.32%), Query Frame = 0

Query: 1   MSPPCILSAVAA---FSLLLPAVSAD--QAPPPPPAAANQNPQRRGSLAREFLQAHNKVR 60
           M+P  +LSAV A    SLL  A + +    P    A A     RR +   EFL+ HNK R
Sbjct: 1   MAPSHVLSAVVAASSLSLLFLAATVETLTLPVATNALAPFLTNRRRTYREEFLEMHNKYR 60

Query: 61  STFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGHWTPSAVVESWA 120
               +PPLTWD+NLTR+ARRWG KRA DCK+ HS+GPYGEN+FWG+L HWTPS VVESW 
Sbjct: 61  GMVNLPPLTWDRNLTRYARRWGAKRAVDCKIVHSFGPYGENMFWGRLDHWTPSKVVESWG 120

Query: 121 GESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVICEYDPPGNYVNE 179
            E + Y++N+N+C AGQMCGHYTQIIW+ETLRLGCAR+ C+NGE LVICEYDPPGNYV+E
Sbjct: 121 EEGQHYHMNNNQCDAGQMCGHYTQIIWKETLRLGCARIRCLNGEFLVICEYDPPGNYVSE 180

BLAST of Lag0027114.1 vs. NCBI nr
Match: XP_023005650.1 (pathogenesis-related protein 1C-like [Cucurbita maxima])

HSP 1 Score: 244.6 bits (623), Expect = 6.5e-61
Identity = 114/183 (62.30%), Postives = 137/183 (74.86%), Query Frame = 0

Query: 1   MSPPCILSA-VAAFSLLLPAVSADQAPPPPPAAANQ----NPQRRGSLAREFLQAHNKVR 60
           M+P  ILSA VA  SL L  ++A       P A N        RR +   EFL+ HNK R
Sbjct: 1   MAPSYILSAVVAGISLSLLFLAATVETLTLPVATNALVPFLTNRRRTYREEFLEVHNKYR 60

Query: 61  STFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGHWTPSAVVESWA 120
           +   +PPLTWD+NLTR+ARRWG KRA DCK+ HS+GPYGEN+FWG+L HWTPS VV+SW 
Sbjct: 61  AMVNLPPLTWDRNLTRYARRWGAKRAIDCKIVHSFGPYGENMFWGRLDHWTPSKVVDSWG 120

Query: 121 GESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVICEYDPPGNYVNE 179
            E + Y++N+N+C AGQMCGHYTQIIW+ETLRLGCAR+ C+NGE LVICEYDPPGNYV+E
Sbjct: 121 EEGQHYHMNNNQCDAGQMCGHYTQIIWKETLRLGCARIRCLNGEFLVICEYDPPGNYVSE 180

BLAST of Lag0027114.1 vs. NCBI nr
Match: KAG6596435.1 (hypothetical protein SDJN03_09615, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027976.1 hypothetical protein SDJN02_09155, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 244.2 bits (622), Expect = 8.5e-61
Identity = 112/183 (61.20%), Postives = 137/183 (74.86%), Query Frame = 0

Query: 1   MSPPCILSAV---AAFSLLLPAVSAD--QAPPPPPAAANQNPQRRGSLAREFLQAHNKVR 60
           M+P  +LSAV   ++ SLL  A + +    P    A A     RR +   EFL+ HNK R
Sbjct: 1   MAPSHVLSAVVTASSLSLLFLAATVETLTLPVATNALAPFLTNRRRTYREEFLEMHNKYR 60

Query: 61  STFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGHWTPSAVVESWA 120
               +PPLTWD+NLTR+ARRWG KRA DCK+ HS+GPYGEN+FWG+L HWTPS VVESW 
Sbjct: 61  GMVNLPPLTWDRNLTRYARRWGAKRAVDCKIVHSFGPYGENMFWGRLDHWTPSKVVESWG 120

Query: 121 GESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVICEYDPPGNYVNE 179
            E + Y++N+N+C AGQMCGHYTQIIW+ETLRLGCAR+ C+NGE LVICEYDPPGNYV+E
Sbjct: 121 EEGQHYHMNNNQCDAGQMCGHYTQIIWKETLRLGCARIRCLNGEFLVICEYDPPGNYVSE 180

BLAST of Lag0027114.1 vs. ExPASy Swiss-Prot
Match: P09042 (Pathogenesis-related protein 1C OS=Nicotiana tabacum OX=4097 PE=2 SV=3)

HSP 1 Score: 162.2 bits (409), Expect = 5.6e-39
Identity = 64/133 (48.12%), Postives = 88/133 (66.17%), Query Frame = 0

Query: 45  REFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGH 104
           +++L AHN  R+   V PLTWD  +  +A+ +  + AADC + HS+G YGENL WG    
Sbjct: 35  QDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLAADCNLVHSHGQYGENLAWGSGDF 94

Query: 105 WTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVIC 164
            T +  VE W  E ++Y  +SN CA GQ+CGHYTQ++WR ++R+GCARV C NG  +V C
Sbjct: 95  LTAAKAVEMWVNEKQYYAHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVQCNNGGYIVSC 154

Query: 165 EYDPPGNYVNEKP 178
            YDPPGN + + P
Sbjct: 155 NYDPPGNVIGKSP 167

BLAST of Lag0027114.1 vs. ExPASy Swiss-Prot
Match: P08299 (Pathogenesis-related protein 1A OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.8e-38
Identity = 65/133 (48.87%), Postives = 88/133 (66.17%), Query Frame = 0

Query: 45  REFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGH 104
           +++L AHN  R+   V PLTWD  +  +A+ +  + AADC + HS+G YGENL  G    
Sbjct: 35  QDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLAADCNLVHSHGQYGENLAEGSGDF 94

Query: 105 WTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVIC 164
            T +  VE W  E ++Y+ +SN CA GQ+CGHYTQ++WR ++R+GCARV C NG  +V C
Sbjct: 95  MTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVQCNNGGYVVSC 154

Query: 165 EYDPPGNYVNEKP 178
            YDPPGNY  E P
Sbjct: 155 NYDPPGNYRGESP 167

BLAST of Lag0027114.1 vs. ExPASy Swiss-Prot
Match: P07053 (Pathogenesis-related protein 1B OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.4e-37
Identity = 63/133 (47.37%), Postives = 88/133 (66.17%), Query Frame = 0

Query: 45  REFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGPYGENLFWGKLGH 104
           +++L AHN  R+   V PLTWD  +  +A+ +  + AADC + HS+G YGENL  G    
Sbjct: 35  QDYLDAHNTARADVGVEPLTWDNGVAAYAQNYVSQLAADCNLVHSHGQYGENLAQGSGDF 94

Query: 105 WTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVIC 164
            T +  VE W  E ++Y+ +SN CA GQ+CGHYTQ++WR ++R+GCARV C NG  +V C
Sbjct: 95  MTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVVWRNSVRVGCARVKCNNGGYVVSC 154

Query: 165 EYDPPGNYVNEKP 178
            YDPPGN + + P
Sbjct: 155 NYDPPGNVIGQSP 167

BLAST of Lag0027114.1 vs. ExPASy Swiss-Prot
Match: Q41359 (Pathogenesis-related protein PR-1 type OS=Sambucus nigra OX=4202 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.5e-36
Identity = 62/134 (46.27%), Postives = 87/134 (64.93%), Query Frame = 0

Query: 45  REFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSYGP-YGENLFWGKLG 104
           ++++ AHN  RS   V P+TWD+++  FAR++   RA DC++ HS  P YGENL +G   
Sbjct: 33  QDYVDAHNAARSAVNVGPVTWDESVAAFARQYAQSRAGDCRLVHSGDPRYGENLAFGSGF 92

Query: 105 HWTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLGCARVNCVNGELLVI 164
             T    V+ W  E   YN N+N CA G++CGHYTQ++WR ++R+GCARV C NG   + 
Sbjct: 93  ELTGRNAVDMWVAERNDYNPNTNTCAPGKVCGHYTQVVWRNSVRIGCARVRCNNGAWFIT 152

Query: 165 CEYDPPGNYVNEKP 178
           C Y PPGNY  ++P
Sbjct: 153 CNYSPPGNYAGQRP 166

BLAST of Lag0027114.1 vs. ExPASy Swiss-Prot
Match: P35792 (Pathogenesis-related protein PRB1-2 OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 66/149 (44.30%), Postives = 91/149 (61.07%), Query Frame = 0

Query: 31  AAANQNPQRRGSLAREFLQAHNKVRSTFKVPPLTWDKNLTRFARRWGDKRAADCKMTHSY 90
           AAA  N  +  +  ++++  HN  RS   V  ++W   L  FA+ + ++R  DCK+ HS 
Sbjct: 15  AAAMVNLSQAQNSPQDYVSPHNAARSAVGVGAVSWSTKLQAFAQNYANQRINDCKLQHSG 74

Query: 91  GPYGENLFWGKLG-HWTPSAVVESWAGESKFYNLNSNECAAGQMCGHYTQIIWRETLRLG 150
           GPYGEN+FWG  G  W  +  V SW  E K YN  SN CAAG++CGHYTQ++WR +  +G
Sbjct: 75  GPYGENIFWGSAGADWKAADAVNSWVNEKKDYNYGSNTCAAGKVCGHYTQVVWRASTSIG 134

Query: 151 CARVNCVNGE-LLVICEYDPPGNYVNEKP 178
           CARV C N   + + C Y+P GN V +KP
Sbjct: 135 CARVVCNNNRGVFITCNYEPRGNIVGQKP 163