Lag0026831.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0026831.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr10: 42369342 .. 42371176 (+)
Sequence length933
RNA-Seq ExpressionLag0026831.1
SyntenyLag0026831.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGACGGATGGATTTTGCCATATTTGTGGGGGAGAATTAGAGACAGTGAACCATGCCCTTCTGGAGTGTGAGCCGACCAGGGCCTTTTGGTTTGGTTCACCACTACAGTTGGACACAAGAGGATATAAGGGTTTGAGTTTTGCTGTGAGTTGGGAGAAAATATATCTAAGAATGGAAGGGAAGGATAATTCGGATGAGGCCCTCTGTTACTTAGGGTATGGGTTGTGGAGGGTTTGGAAGGAAAGGAATGCGGTGATTTTCAAAAACAAGGCCCCGGAAATTGAGAATAACATTGTATTCACCATCAGAGATGTTGGTGAATACAGAGAAGCTGTGGGAGAGGCAGCAATTGGTAGGCGAGAAGAGCCGGGTAGTGAGGATAGGTAGGTGGGTCGAAGTCCAGATCGGCCGACTTTGTGGATAGATGCTACATGGATAAAGGAGACTTTATCGTCTGGACTGGGCTGGTCATTGCGAGGCCCTGATGGAAGCATTTGGCTAGAACAAGCAGAAAATGGTGGGAGCAGTTTGAACAGCATGCATGCAGAGGGAAAAGCATTAGTTCGTGGCCTGGAATGCGCGAAGATGATGGGCATTCAAGAGCTGCTTGTTAAGTTTGATGCTTTGCGAGTGGTGAACATTATGAATGGGAAGGAAAAATGCCCAGTGGACTTTGAACCAATATATGACTGCTTTTGTCATCTACTTGAGGATTTTAATTCTTGCGAATTGTTATTTGTCAAGAGGGAGCTCAATGAGAGAGCTCATATGTTAGCCAAGAATGGCCTTTGTAATTAGTTATTTCTTGGAATAAAGCTTTCGTTTGGAAAAAAAAACAAAAAAACAAAATCTTATTAAGTATTCAAAAAAACAAAATCTTATAAAAAAAAAAATAATCTCAACTAAGCCAATTTATTTTGTAGAAACTTGTAGATTTAATAAAGTTTTATGGTTAAAAGTTGGAAAACTAATTGTTGGGGAGGTAAAGTGTAAAAGGTTGGTAAATGTTTAGCCTTCAAACAATAATTAATATAACATAAAATTGAATTAAATAATAAGATCAAAAGAAAAGGGCAAAACAACCCCAAAAAAATTAATTTTAAATTACTTTTATTAATTTTTGGGTAGGTTTCAATAATGGCTTGGAAAACTTACAAAGTTTCATTTTTACTCTCTTCAATTTGTTTCAAAAAATAACCTTTAGTCAACTTTAATTTTGTGTGAAAATGAAGATAGAAAAGTGATGCGAGTTGACAAAGGTGACCATATTTCAATAATGCGCTTGAACTTTAAGATGCTTTTCAGTTAAACATTATTTATCATTTCATAATTTTCATTTCAACCTTTAACATTTGGAATTATTTGAAAACAACTATTTTATTAGCTTCTCCTACTTAAAATGAAGACGAAAATATAATGTTGCAATTAGCTGGAAAAAGAAAGGTCATACGTGGGTGTATTATGTTGCACTACTACAAAATCCATATTACTTGATGTTCACACGTAACTTCAGGGATGGAGACACGTTGAATAAACGATATACTTGTATTAATATTTGTATGCGTGTGGATGTACAATCTCTAATGTTTGTCCTCTGATTCTCTCTCTTGTGTGTGGAAGTGTCTCACTCGAAGGCTGGAATGCTTGATCTCGAGTCTGAAGCTTTAATGGATTCTTGGATCTTGAAGTCTTCCGATCTTGAAGGAGTCTTCAATCTTCAAGAAGTGTTCAGCACTTTGAAACTTGTGGATTTCAAGGAGTCTTCAGTCTTCAGTCTTGATGAAGTTTTCTCTGAATTCTCTGACTCTGGCTTGGAGCTTCCAAAATTCTGA

mRNA sequence

ATGCAGACGGATGGATTTTGCCATATTTGTGGGGGAGAATTAGAGACAGTGAACCATGCCCTTCTGGAGTGTGAGCCGACCAGGGCCTTTTGGTTTGGTTCACCACTACAGTTGGACACAAGAGGATATAAGGGTTTGAGTTTTGCTGTGAGTTGGGAGAAAATATATCTAAGAATGGAAGGGAAGGATAATTCGGATGAGGCCCTCTGTTACTTAGGGTATGGGTTGTGGAGGGTTTGGAAGGAAAGGAATGCGGTGATTTTCAAAAACAAGGCCCCGGAAATTGAGAATAACATTGTATTCACCATCAGAGATGTTGGTGAATACAGAGAAGCTGTGGGAGAGGCAGCAATTGATGCTACATGGATAAAGGAGACTTTATCGTCTGGACTGGGCTGGTCATTGCGAGGCCCTGATGGAAGCATTTGGCTAGAACAAGCAGAAAATGGTGGGAGCAGTTTGAACAGCATGCATGCAGAGGGAAAAGCATTAGTTCGTGGCCTGGAATGCGCGAAGATGATGGGCATTCAAGAGCTGCTTGTTAAGTTTGATGCTTTGCGAGTGGTGAACATTATGAATGGGAAGGAAAAATGCCCAGTGGACTTTGAACCAATATATGACTGCTTTTGTCATCTACTTGAGGATTTTAATTCTTGCGAATTGTTATTTGTCAAGAGGGAGCTCAATGAGAGAGCTCATATGTTAGCCAAGAATGGCCTTTTGTCTCACTCGAAGGCTGGAATGCTTGATCTCGAGTCTGAAGCTTTAATGGATTCTTGGATCTTGAAGTCTTCCGATCTTGAAGGAGTCTTCAATCTTCAAGAAGTGTTCAGCACTTTGAAACTTGTGGATTTCAAGGAGTCTTCAGTCTTCAGTCTTGATGAAGTTTTCTCTGAATTCTCTGACTCTGGCTTGGAGCTTCCAAAATTCTGA

Coding sequence (CDS)

ATGCAGACGGATGGATTTTGCCATATTTGTGGGGGAGAATTAGAGACAGTGAACCATGCCCTTCTGGAGTGTGAGCCGACCAGGGCCTTTTGGTTTGGTTCACCACTACAGTTGGACACAAGAGGATATAAGGGTTTGAGTTTTGCTGTGAGTTGGGAGAAAATATATCTAAGAATGGAAGGGAAGGATAATTCGGATGAGGCCCTCTGTTACTTAGGGTATGGGTTGTGGAGGGTTTGGAAGGAAAGGAATGCGGTGATTTTCAAAAACAAGGCCCCGGAAATTGAGAATAACATTGTATTCACCATCAGAGATGTTGGTGAATACAGAGAAGCTGTGGGAGAGGCAGCAATTGATGCTACATGGATAAAGGAGACTTTATCGTCTGGACTGGGCTGGTCATTGCGAGGCCCTGATGGAAGCATTTGGCTAGAACAAGCAGAAAATGGTGGGAGCAGTTTGAACAGCATGCATGCAGAGGGAAAAGCATTAGTTCGTGGCCTGGAATGCGCGAAGATGATGGGCATTCAAGAGCTGCTTGTTAAGTTTGATGCTTTGCGAGTGGTGAACATTATGAATGGGAAGGAAAAATGCCCAGTGGACTTTGAACCAATATATGACTGCTTTTGTCATCTACTTGAGGATTTTAATTCTTGCGAATTGTTATTTGTCAAGAGGGAGCTCAATGAGAGAGCTCATATGTTAGCCAAGAATGGCCTTTTGTCTCACTCGAAGGCTGGAATGCTTGATCTCGAGTCTGAAGCTTTAATGGATTCTTGGATCTTGAAGTCTTCCGATCTTGAAGGAGTCTTCAATCTTCAAGAAGTGTTCAGCACTTTGAAACTTGTGGATTTCAAGGAGTCTTCAGTCTTCAGTCTTGATGAAGTTTTCTCTGAATTCTCTGACTCTGGCTTGGAGCTTCCAAAATTCTGA

Protein sequence

MQTDGFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDNSDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREAVGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLNSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLEDFNSCELLFVKRELNERAHMLAKNGLLSHSKAGMLDLESEALMDSWILKSSDLEGVFNLQEVFSTLKLVDFKESSVFSLDEVFSEFSDSGLELPKF
Homology
BLAST of Lag0026831.1 vs. NCBI nr
Match: CAB4273082.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 123.6 bits (309), Expect = 2.9e-24
Identity = 72/263 (27.38%), Postives = 115/263 (43.73%), Query Frame = 0

Query: 7    CHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDNSD 66
            C +CG E E+  H   +CE  R  WF SP+QLD     G  F V WE +  + E  ++ +
Sbjct: 1017 CALCGQEGESQVHVFFKCEFARLLWFASPIQLDPCHILGEDFIVCWENLLKKYEQVEHCN 1076

Query: 67   EALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA-------------- 126
            + L    +GLWR+WK RN  +F+    E    +   +  V E+R A              
Sbjct: 1077 DILQVCAFGLWRIWKIRNGAVFEGTVIEPHVAVECFLDQVHEFRTAQMHGKPLINELPRP 1136

Query: 127  -----------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSL- 186
                             V +   DA W+ +T   G+GW +R  DG  W+ QA   G    
Sbjct: 1137 PEVQAETVTSWRKPPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDG--WMVQAGGKGDMRG 1196

Query: 187  -NSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLL 237
             +++  E +A+   L      G+ +L V+ D+L+V+ ++ G+    +  + I      L+
Sbjct: 1197 GSALAMEAEAIREALVACVQSGLSKLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLV 1256

BLAST of Lag0026831.1 vs. NCBI nr
Match: XP_028960018.1 (uncharacterized protein LOC114825459 [Malus domestica])

HSP 1 Score: 120.2 bits (300), Expect = 3.2e-23
Identity = 77/271 (28.41%), Postives = 116/271 (42.80%), Query Frame = 0

Query: 1    MQTDGFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRME 60
            M+ D  C +CG   ET NH    CE +  FWF SPLQL++   +G+ F  SW     R++
Sbjct: 864  MRVDNVCGVCGQFDETENHLFFRCETSHRFWFCSPLQLNSIELEGVDFLQSWASFCNRVD 923

Query: 61   GKDNSDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREAVGEAA--- 120
              DN  E L    +GLWR+WK RN +IF +K  E    ++   R + EYR A  +     
Sbjct: 924  QLDNRMELLQEFVFGLWRLWKNRNDIIFNHKNHEPAEILMDWRRSLAEYRNAAEKREQSM 983

Query: 121  ----------------------------IDATWIKETLSSGLGWSLRGPDGSIWLEQAEN 180
                                         DA W K T  +GLGW      G +   Q   
Sbjct: 984  KPVLTKVAHDGAGQKHWHKPGFGTIKINTDAAWSKVTQQAGLGWVACDFAGVL---QGAG 1043

Query: 181  GGSSL---NSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIY 238
            G  S+   ++  AE  A+   LE   + G + ++++ DA  +V ++  +       E + 
Sbjct: 1044 GSGSIRFHSAAAAEVAAIREALEDCLLRGFRNVIIESDAKTIVQMIRNELTQDFRLECLL 1103

BLAST of Lag0026831.1 vs. NCBI nr
Match: CAB4303756.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 117.5 bits (293), Expect = 2.1e-22
Identity = 66/259 (25.48%), Postives = 114/259 (44.02%), Query Frame = 0

Query: 5   GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
           G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 669 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 728

Query: 65  SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
           + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 729 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEAVLLLLKQVSEFRTAKQDLQPQPLQPV 788

Query: 125 --------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLNSMH 184
                         + +   DA W  + +  G+GW +R   G +     +      +++ 
Sbjct: 789 SSPAPLSWCKPPPGLLKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIM 848

Query: 185 AEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLEDFN 237
            E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+   +
Sbjct: 849 MELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLS 908

BLAST of Lag0026831.1 vs. NCBI nr
Match: XP_028949366.1 (uncharacterized protein LOC114821432 [Malus domestica])

HSP 1 Score: 116.3 bits (290), Expect = 4.7e-22
Identity = 71/270 (26.30%), Postives = 117/270 (43.33%), Query Frame = 0

Query: 1   MQTDGFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRME 60
           M+ D  C +C    ET NH    C  +  FWF SPL L++   +G  F  SW K +  M+
Sbjct: 1   MRVDNVCGVCNAVDETENHIFFRCRFSHIFWFCSPLHLNSHELEGFDFLDSWGKFHEGMK 60

Query: 61  GKDNSDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA---VGEAA 120
           GK+N++E      +GLWR+WK RN  +FK    +  + +    +++ E+REA   +G+ A
Sbjct: 61  GKENAEEICQEFAFGLWRLWKNRNDAVFKGLYRQPLDVMDLWWKNIIEFREASTSLGDDA 120

Query: 121 -----------------------------IDATWIKETLSSGLGWSLRGPDGSIWLEQAE 180
                                         DA W K TL +G+GW  R   G +      
Sbjct: 121 WNMDVTPLSATARPSLHWQRPRYGTIKINTDAAWCKVTLRAGVGWVGRDFAGVLQAAGGM 180

Query: 181 NGGSSLNSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDC 239
                L++  AE  A+   L+         ++++ DA  ++ ++  +     + + +   
Sbjct: 181 GTVRCLSAAAAEAIAIRNALKACITHRFNHVVIESDAKLIIQMIRKEVTVDCNLDCVIGD 240

BLAST of Lag0026831.1 vs. NCBI nr
Match: CAB4274760.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 115.9 bits (289), Expect = 6.1e-22
Identity = 66/262 (25.19%), Postives = 114/262 (43.51%), Query Frame = 0

Query: 5    GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
            G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 997  GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 1056

Query: 65   SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
            + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 1057 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEVVLLLLKQVSEFRTAKHDLQDLQPQPL 1116

Query: 125  -----------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLN 184
                             + +   DA W  + +  G+GW +R   G +     +      +
Sbjct: 1117 QPASSPAPLSWCKPSLGLLKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSS 1176

Query: 185  SMHAEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLE 237
            ++  E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+ 
Sbjct: 1177 AIMMELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVA 1236

BLAST of Lag0026831.1 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-24
Identity = 72/263 (27.38%), Postives = 115/263 (43.73%), Query Frame = 0

Query: 7    CHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDNSD 66
            C +CG E E+  H   +CE  R  WF SP+QLD     G  F V WE +  + E  ++ +
Sbjct: 1017 CALCGQEGESQVHVFFKCEFARLLWFASPIQLDPCHILGEDFIVCWENLLKKYEQVEHCN 1076

Query: 67   EALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA-------------- 126
            + L    +GLWR+WK RN  +F+    E    +   +  V E+R A              
Sbjct: 1077 DILQVCAFGLWRIWKIRNGAVFEGTVIEPHVAVECFLDQVHEFRTAQMHGKPLINELPRP 1136

Query: 127  -----------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSL- 186
                             V +   DA W+ +T   G+GW +R  DG  W+ QA   G    
Sbjct: 1137 PEVQAETVTSWRKPPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDG--WMVQAGGKGDMRG 1196

Query: 187  -NSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLL 237
             +++  E +A+   L      G+ +L V+ D+L+V+ ++ G+    +  + I      L+
Sbjct: 1197 GSALAMEAEAIREALVACVQSGLSKLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLV 1256

BLAST of Lag0026831.1 vs. ExPASy TrEMBL
Match: A0A6J5WPU6 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS20372 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.0e-22
Identity = 66/259 (25.48%), Postives = 114/259 (44.02%), Query Frame = 0

Query: 5   GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
           G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 669 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 728

Query: 65  SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
           + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 729 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEAVLLLLKQVSEFRTAKQDLQPQPLQPV 788

Query: 125 --------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLNSMH 184
                         + +   DA W  + +  G+GW +R   G +     +      +++ 
Sbjct: 789 SSPAPLSWCKPPPGLLKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIM 848

Query: 185 AEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLEDFN 237
            E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+   +
Sbjct: 849 MELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLS 908

BLAST of Lag0026831.1 vs. ExPASy TrEMBL
Match: A0A6J5UE59 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23353 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-22
Identity = 66/262 (25.19%), Postives = 114/262 (43.51%), Query Frame = 0

Query: 5    GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
            G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 997  GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 1056

Query: 65   SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
            + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 1057 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEVVLLLLKQVSEFRTAKHDLQDLQPQPL 1116

Query: 125  -----------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLN 184
                             + +   DA W  + +  G+GW +R   G +     +      +
Sbjct: 1117 QPASSPAPLSWCKPSLGLLKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSS 1176

Query: 185  SMHAEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLE 237
            ++  E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+ 
Sbjct: 1177 AIMMELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVA 1236

BLAST of Lag0026831.1 vs. ExPASy TrEMBL
Match: A0A6J5UF50 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23354 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-22
Identity = 66/262 (25.19%), Postives = 114/262 (43.51%), Query Frame = 0

Query: 5   GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
           G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 129 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 188

Query: 65  SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
           + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 189 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEVVLLLLKQVSEFRTAKHDLQDLQPQPL 248

Query: 125 -----------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLN 184
                            + +   DA W  + +  G+GW +R   G +     +      +
Sbjct: 249 QPASSPAPLSWCKPSLGLLKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSS 308

Query: 185 SMHAEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLE 237
           ++  E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+ 
Sbjct: 309 AIMMELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVA 368

BLAST of Lag0026831.1 vs. ExPASy TrEMBL
Match: A0A6J5YDN0 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS50956 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.6e-22
Identity = 64/256 (25.00%), Postives = 111/256 (43.36%), Query Frame = 0

Query: 5   GFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDN 64
           G C  CG + ET  H   EC+  RAFWF SPLQLD     G  F  +W+ +   +   ++
Sbjct: 224 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 283

Query: 65  SDEALCYLGYGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------------ 124
           + EA+ +  +GLWR+WK RN  +F     +    ++  ++ V E+R A            
Sbjct: 284 AAEAIQWFVFGLWRIWKARNLAVFDGVQADPTEAVLLLLKQVSEFRTAKQDLQPQPLQPV 343

Query: 125 --------------VGEAAIDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGSSLNSMH 184
                         + +   DA W  + +  G+GW +R     +     +      +++ 
Sbjct: 344 SSPAPLSWCKPPPGLLKVNCDAAWSAQRMGGGVGWVIRDSFSLLLCAGGQGDLHGSSALM 403

Query: 185 AEGKALVRGL-ECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLLEDFN 234
            E  A+   L ECA    + +++V+ D+ + + ++NG+     D E I      L+   +
Sbjct: 404 MELLAIRHALSECAN-FHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLS 463

BLAST of Lag0026831.1 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 5.3e-16
Identity = 68/268 (25.37%), Postives = 105/268 (39.18%), Query Frame = 0

Query: 7   CHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSW-EKIYLRMEGKDNS 66
           C  C    ETVNH L +C   R  W  SP+     G         W + +Y  +    N 
Sbjct: 12  CVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPEG--------EWTDSLYANLYWVLNL 71

Query: 67  DEALCYLG-------YGLWRVWKERNAVIFKNK---APEIENNIVFTIRDVGEYREAVGE 126
           +  +  LG       + LWR+WK RN ++FK K   APE+    +    +    RE  G+
Sbjct: 72  EVEIPKLGKIGNLVPWLLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGK 131

Query: 127 AA-----------------------IDATWIKETLSSGLGWSLRGPDGSIWLEQAENGGS 186
           A+                        DATW  E    G+GW LR   G +    A     
Sbjct: 132 ASGPQVERNLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPR 191

Query: 187 SLNSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHL 241
           + N + AE +AL   +        + ++ + DA  +VN++N  +  P   +P  +    L
Sbjct: 192 TKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPT-LQPALEDIQQL 251

BLAST of Lag0026831.1 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.9e-13
Identity = 68/297 (22.90%), Postives = 120/297 (40.40%), Query Frame = 0

Query: 1   MQTDGFCHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRME 60
           +  D  C  C  E ET++H +  C  T++ W  + + +  +     SF  +  ++ +++ 
Sbjct: 69  IDADPICQRCCIEEETIHHIMFNCPYTQSVWRSANIIIGNQWGPPSSFEDNLNRL-IQLS 128

Query: 61  GKDNSDEALCYLGYG-LWRVWKERNAVIFKNKAPEIENNIVFTIRDVGEYREA------- 120
               ++    +L +  +WR+WK RN  +F+ K    +      I+D  E+  A       
Sbjct: 129 KTQTTNSLDRFLPFWIMWRLWKSRNVFLFQQKCQSPDYEARKGIQDATEWLNANETTENT 188

Query: 121 --------VGEAAIDAT--------WIKETLSSGL---------GWSLRGPDGSIWLEQA 180
                   +  +  D++        W+K    SG          GW++R  +G I L   
Sbjct: 189 NVHVATNPIQTSRRDSSQWNPPPEGWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGN 248

Query: 181 ENGGSSLNSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYD 240
               SS  S+HAE    +  L+     G++ +  + D+  +V ++N  E   +    IYD
Sbjct: 249 AKLQSSTCSLHAEALGFLHALQVIWAHGLRYVWFESDSKSLVTLINNGEDHSLLGTLIYD 308

Query: 241 CFCHLLEDFNSCELLFVKRELNERAHMLAKNGLLSHSKAGMLDLESEALMDSWILKS 265
              H +     C L FV RE N  A  LA     SH  A     +S     SW++ +
Sbjct: 309 -IRHWMLKLPYCSLEFVNRERNSAADALA-----SHVHARDPLFQSYTTPPSWLVNT 358

BLAST of Lag0026831.1 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-10
Identity = 61/267 (22.85%), Postives = 109/267 (40.82%), Query Frame = 0

Query: 7   CHICGGELETVNHALLECEPTRAFWFGSPLQLDTRGYKGLSFAVSWEKIYLRMEGKDNSD 66
           CH C  E ET  H   +C   +  W  S +        G++     E   L      N  
Sbjct: 56  CHRCCQEDETSQHLFFDCFYAQQVWRASGIPHQELRTTGITMETKME--LLLSSCLANRQ 115

Query: 67  EALCYLG-YGLWRVWKERNAVIFKNKAPEIENNIVFTIRDVGE------YREAVGEAAID 126
             L  L  + LWR+WK RN ++F+ K+   +N +     DV E      Y +++ +    
Sbjct: 116 PQLFNLAIWILWRLWKSRNQLVFQQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHS 175

Query: 127 -----------------ATWIK---------ETLSSGLGWSLRGPDGSIWLEQAENGGSS 186
                            +TWIK         +T ++  GW +R  +G         G ++
Sbjct: 176 SRHQQPTMARTKWQRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTT 235

Query: 187 LNSMHAEGKALVRGLECAKMMGIQELLVKFDALRVVNIMNGKEKCPVDFEPIYDCFCHLL 241
            +S+ +E +AL+  ++ A   G ++++ + D+ +V  +MN ++     F  I +      
Sbjct: 236 SDSLESEFQALIIAMQHAWSQGYRKVIFEGDSKQVEELMNNEKLNFGRFNWIRE-GRFWQ 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CAB4273082.12.9e-2427.38unnamed protein product [Prunus armeniaca][more]
XP_028960018.13.2e-2328.41uncharacterized protein LOC114825459 [Malus domestica][more]
CAB4303756.12.1e-2225.48unnamed protein product [Prunus armeniaca][more]
XP_028949366.14.7e-2226.30uncharacterized protein LOC114821432 [Malus domestica][more]
CAB4274760.16.1e-2225.19unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J5UAY21.4e-2427.38Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5WPU61.0e-2225.48Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5UE592.9e-2225.19Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5UF502.9e-2225.19Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23354 PE=4 S... [more]
A0A6J5YDN08.6e-2225.00Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS50956 PE=... [more]
Match NameE-valueIdentityDescription
AT2G34320.15.3e-1625.37Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G02650.11.9e-1322.90Ribonuclease H-like superfamily protein [more]
AT3G25270.12.6e-1022.85Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 119..238
e-value: 2.3E-20
score: 72.6
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 115..247
e-value: 3.9E-15
score: 58.0
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 117..252
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 117..252
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 118..237
e-value: 1.53549E-15
score: 69.6504
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 118..241

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0026831Lag0026831gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0026831.1.exon1Lag0026831.1.exon1exon
Lag0026831.1.exon2Lag0026831.1.exon2exon
Lag0026831.1.exon3Lag0026831.1.exon3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0026831.1cds.Lag0026831.1CDS
cds.Lag0026831.1cds.Lag0026831.1_2CDS
cds.Lag0026831.1cds.Lag0026831.1_3CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0026831.1Lag0026831.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity