Lag0024953.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0024953.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr10: 7236265 .. 7237158 (-)
Sequence length	801
RNA-Seq Expression	Lag0024953.1
Synteny	Lag0024953.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAACAGAATCAAAGCAAGGTCAAGTACTAACTCTATGGCGAAAGTCTGGTCTAACCTTTGGAGTTTGAAGGTTCCTGCCAAAGTGAAACACTTTTTATGGAGAGCTCTTAATGATGTTATCCCCACTAATGTGAATTTATACCGAAGAGGTATTCAAACCAGTGTTATGTGCCCTAACTTGGTAGCTGAAACTACTGATCACAACTTAGTTGGATGTATGAAGGCACGGGAAGTTTGGGATCTTACCTTTGCCCATGATCTTTTAAAAACCAAGCTCAATCACAGTTTTGCTGACAAATGGTTTGCGATGAATTCTTTCATTTCTATCGAGGATCTCCAGAAGGTTGCCATTACCTGTTGGTCAAATTGGGCAGACAGAAATAAGTTAGTCCATAATAAGCCAATTCCCCTTCCTTTGATTCGAAGCCAATTGATTAAAAAATACGATTCAGAATACCTTCAAATGAATGGGCCTCTATCATCCAATTCGAGTAGGCAAGCTCCTAATAGAGCTCCAGTGTCCTATTGGAAGCCCCCGACGACTGATTACTAGAAGATTAATGTTGATGCGGCTTGGAAAGCAGGCGATCCCTATACTGGTATAGAGATTGTTAGCAGAAATTCTATCGGCAAACTTATTGGCGCCTCATCAGTTCATATAGATATGAATTTTCCTCCTCCCATGGCTGAGCTTATGGCAATTGTGGAAGGGTTGAAATTTGCTGAGCAATTAGGGCATGATCGAATTAAGCTGGAATCAGACTGTCTCCAAGCTATCAGTCTTATAAACCGGGATGCTGAAGTAAGAAATGAGATTGGGACTTTGATTGAGGATATCAAGAGACGAACAACTGTTTTCTCGGGGATTCTTTTTTTCTCATGTGCCTAG

mRNA sequence

ATGAAGAACAGAATCAAAGCAAGGTCAAGTACTAACTCTATGGCGAAAGTCTGGTCTAACCTTTGGAGTTTGAAGGTTCCTGCCAAAGTGAAACACTTTTTATGGAGAGCTCTTAATGATGTTATCCCCACTAATGTGAATTTATACCGAAGAGGTATTCAAACCAGTGTTATGTGCCCTAACTTGGTAGCTGAAACTACTGATCACAACTTAGTTGGATGTATGAAGGCACGGGAAGTTTGGGATCTTACCTTTGCCCATGATCTTTTAAAAACCAAGCTCAATCACAGTTTTGCTGACAAATGGTTTGCGATGAATTCTTTCATTTCTATCGAGGATCTCCAGAAGGTTGCCATTACCTGTTGGTCAAATTGGGCAGACAGAAATAAGTTAGTCCATAATAAGCCAATTCCCCTTCCTTTGATTCGAAGCCAATTGATTAAAAAATACGATTCAGAATACCTTCAAATGAATGGGCCTCTATCATCCAATTCGAGCGATCCCTATACTGGTATAGAGATTGTTAGCAGAAATTCTATCGGCAAACTTATTGGCGCCTCATCAGTTCATATAGATATGAATTTTCCTCCTCCCATGGCTGAGCTTATGGCAATTGTGGAAGGGTTGAAATTTGCTGAGCAATTAGGGCATGATCGAATTAAGCTGGAATCAGACTGTCTCCAAGCTATCAGTCTTATAAACCGGGATGCTGAAGTAAGAAATGAGATTGGGACTTTGATTGAGGATATCAAGAGACGAACAACTGTTTTCTCGGGGATTCTTTTTTTCTCATGTGCCTAG

Coding sequence (CDS)

ATGAAGAACAGAATCAAAGCAAGGTCAAGTACTAACTCTATGGCGAAAGTCTGGTCTAACCTTTGGAGTTTGAAGGTTCCTGCCAAAGTGAAACACTTTTTATGGAGAGCTCTTAATGATGTTATCCCCACTAATGTGAATTTATACCGAAGAGGTATTCAAACCAGTGTTATGTGCCCTAACTTGGTAGCTGAAACTACTGATCACAACTTAGTTGGATGTATGAAGGCACGGGAAGTTTGGGATCTTACCTTTGCCCATGATCTTTTAAAAACCAAGCTCAATCACAGTTTTGCTGACAAATGGTTTGCGATGAATTCTTTCATTTCTATCGAGGATCTCCAGAAGGTTGCCATTACCTGTTGGTCAAATTGGGCAGACAGAAATAAGTTAGTCCATAATAAGCCAATTCCCCTTCCTTTGATTCGAAGCCAATTGATTAAAAAATACGATTCAGAATACCTTCAAATGAATGGGCCTCTATCATCCAATTCGAGCGATCCCTATACTGGTATAGAGATTGTTAGCAGAAATTCTATCGGCAAACTTATTGGCGCCTCATCAGTTCATATAGATATGAATTTTCCTCCTCCCATGGCTGAGCTTATGGCAATTGTGGAAGGGTTGAAATTTGCTGAGCAATTAGGGCATGATCGAATTAAGCTGGAATCAGACTGTCTCCAAGCTATCAGTCTTATAAACCGGGATGCTGAAGTAAGAAATGAGATTGGGACTTTGATTGAGGATATCAAGAGACGAACAACTGTTTTCTCGGGGATTCTTTTTTTCTCATGTGCCTAG

Protein sequence

MKNRIKARSSTNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVAETTDHNLVGCMKAREVWDLTFAHDLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSNWADRNKLVHNKPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYTGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHDRIKLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTVFSGILFFSCA

Homology

[Close]

BLAST of Lag0024953.1 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-24
Identity = 80/285 (28.07%), Postives = 127/285 (44.56%), Query Frame = 0


Query: 9    SSTNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCP--NLVAET 68
            +STN     W+++W L VP K+K F+WR+ ++ IPT  NL  RGI     C       E+
Sbjct: 1123 ASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRES 1182

Query: 69   TDHNLVGCMKAREVWDLTFAH-DLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSNW 128
              H    C +AR++W   F     L  + N SF + W ++   +  +DL   AIT W  W
Sbjct: 1183 IIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIW 1242

Query: 129  ADRNKLVHNKPIPLPLIRSQLIKKYDSEYLQMN----GPLSSNSSDPY------------ 188
             DRN L+H K +     + + +  +   + Q       P + ++  P             
Sbjct: 1243 NDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSL 1302

Query: 189  ------------TGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGH 248
                        T    + R+S   L+ A+S+ +     P +AE+  I+EGLKFA     
Sbjct: 1303 KLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNF 1362

Query: 249  DRIKLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTVFSGILF 263
              +++ESD L AI LI  +   R +    + +I+  T  F+ I F
Sbjct: 1363 THLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISF 1407

[Close]

BLAST of Lag0024953.1 vs. NCBI nr
Match: XP_042973114.1 (uncharacterized protein LOC122304919 [Carya illinoinensis])

HSP 1 Score: 119.0 bits (297), Expect = 6.2e-23
Identity = 79/285 (27.72%), Postives = 129/285 (45.26%), Query Frame = 0


Query: 9   SSTNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCP--NLVAET 68
           S  + ++ VW  LW L     V+ F+W+A N+ +PT  NL RR I T    P   L  ET
Sbjct: 5   SCIDPLSSVWKTLWKLPTTPAVRMFMWKAWNEALPTMANLMRRHIVTESYYPICKLECET 64

Query: 69  TDHNLVGCMKAREVWDLTFAHDLLKTKLN-HSFADKWFAMNSFISIEDLQKVAITCWSNW 128
           T+H L  C  AR+VW       + K  LN  SF + W  ++  +  E+L +VA+ C S W
Sbjct: 65  TEHALWSCEAARDVWS-QCRKKIQKLNLNCVSFKETWGILSQQLPGEELMEVAVICRSLW 124

Query: 129 ADRNKLVHNKPIPLPLIRSQLIKK-------YDSE------------------------- 188
             RN  +H K    P   +Q++ K       Y S                          
Sbjct: 125 TRRNGFIHGKGFMHP---NQVLSKALDEMEAYKSSRDHQSSSPASHGEGSASVWIQPPAG 184

Query: 189 YLQMNGPLSSNSSDPYTGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAE 248
           Y ++N  ++ N++    G+  + R+ +G+ IG      ++N  P   E  A++  + F +
Sbjct: 185 YYKLNWDVAINTARGLVGVGAILRDHMGRSIGTIQARRELNLNPFSGEAYALMMSVVFCK 244

Query: 249 QLGHDRIKLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTVFS 259
            +G  R+ +E D +Q I L++      +  G ++ED KR+   F+
Sbjct: 245 DIGVSRLVVEGDAIQVIRLLSGSVLDCSYGGLVVEDAKRKLNSFA 285

[Close]

BLAST of Lag0024953.1 vs. NCBI nr
Match: CAB4277970.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 115.9 bits (289), Expect = 5.2e-22
Identity = 83/274 (30.29%), Postives = 126/274 (45.99%), Query Frame = 0


Query: 11  TNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVA--ETTD 70
           ++S  K W +LW LKVP K+ H LWR   D++P+   L+RR I    +C    A  ETT 
Sbjct: 273 SSSSLKSWKHLWKLKVPQKIMHLLWRVAQDILPSKEVLFRRRITQGEVCCRCFAARETTL 332

Query: 71  HNLVGCMKAREVWD-LTFAHDLLKTKLNHSFADKWF-AMNSFISIEDLQKVAITCWSNWA 130
           H LVGC    +VW  L F  D L   L  +    W  A+ S I  +     A T W  W 
Sbjct: 333 HALVGCDVCLQVWKALDFPSDFLLPTL--ADVGTWMDAVWSIIPPDKQSLFAFTVWVLWN 392

Query: 131 DRN-KLVHNKPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYTGIE------------ 190
           +RN  L  ++P P  ++  Q  K YD+E+ + +     + S P   I+            
Sbjct: 393 ERNGVLFGSQPTPSGIL-VQRAKDYDAEFKRYSAANHRSLSSPVRDIKWRPPTGNCFKLN 452

Query: 191 ---------------IVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHD 250
                           + R+S GKL+GA ++           EL A+  G+ FA  +   
Sbjct: 453 VDGATDMETGARGAGAIVRDSHGKLVGALAMRAPSRISVLATELYALKIGISFALDMSLV 512

Query: 251 RIKLESDCLQAISLINRDAEVRNEIGTLIEDIKR 253
            +++ESD LQA+S++N + E     G L++ ++R
Sbjct: 513 PLEIESDSLQAVSMVNSEEECLAAEGGLVDGVRR 543

[Close]

BLAST of Lag0024953.1 vs. NCBI nr
Match: CAB4277969.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 115.9 bits (289), Expect = 5.2e-22
Identity = 83/274 (30.29%), Postives = 126/274 (45.99%), Query Frame = 0


Query: 11   TNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVA--ETTD 70
            ++S  K W +LW LKVP K+ H LWR   D++P+   L+RR I    +C    A  ETT 
Sbjct: 1423 SSSSLKSWKHLWKLKVPQKIMHLLWRVAQDILPSKEVLFRRRITQGEVCCRCFAARETTL 1482

Query: 71   HNLVGCMKAREVWD-LTFAHDLLKTKLNHSFADKWF-AMNSFISIEDLQKVAITCWSNWA 130
            H LVGC    +VW  L F  D L   L  +    W  A+ S I  +     A T W  W 
Sbjct: 1483 HALVGCDVCLQVWKALDFPSDFLLPTL--ADVGTWMDAVWSIIPPDKQSLFAFTVWVLWN 1542

Query: 131  DRN-KLVHNKPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYTGIE------------ 190
            +RN  L  ++P P  ++  Q  K YD+E+ + +     + S P   I+            
Sbjct: 1543 ERNGVLFGSQPTPSGIL-VQRAKDYDAEFKRYSAANHRSLSSPVRDIKWRPPTGNCFKLN 1602

Query: 191  ---------------IVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHD 250
                            + R+S GKL+GA ++           EL A+  G+ FA  +   
Sbjct: 1603 VDGATDMETGARGAGAIVRDSHGKLVGALAMRAPSRISVLATELYALKIGISFALDMSLV 1662

Query: 251  RIKLESDCLQAISLINRDAEVRNEIGTLIEDIKR 253
             +++ESD LQA+S++N + E     G L++ ++R
Sbjct: 1663 PLEIESDSLQAVSMVNSEEECLAAEGGLVDGVRR 1693

[Close]

BLAST of Lag0024953.1 vs. NCBI nr
Match: XP_030926547.1 (uncharacterized protein LOC115953156 [Quercus lobata])

HSP 1 Score: 114.8 bits (286), Expect = 1.2e-21
Identity = 78/273 (28.57%), Postives = 126/273 (46.15%), Query Frame = 0


Query: 17  VWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCP--NLVAETTDHNLVGC 76
           +W  LW L +P K+K F WRA  + +PT   +YRRGI  ++ CP     AE+ DH L+ C
Sbjct: 239 LWKRLWHLHLPTKIKVFAWRACVNGLPTMEAIYRRGISQNMACPVCGNDAESVDHALLRC 298

Query: 77  MKAREVWDLTFAHDLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSNWADRNKLVHN 136
             +  VWD    + L      +SF D    + S  +++DL+    T W+ W++RN ++H 
Sbjct: 299 DFSSLVWDFWLENPLHTQGFKNSFLDSALFILSHSTLQDLEIFFATAWAIWSNRNNILHK 358

Query: 137 KPIPLPLIRSQLIKKYDSEY-------LQMNGPLSSNSSDPYTG---------------- 196
                PL      +    E+         +  P SS+ S P  G                
Sbjct: 359 DGGLSPLHVWHRARNVVEEFTCSASWDFDLVRPASSSWSPPPPGAFKVNVDGASSEQEGS 418

Query: 197 --IEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHDRIKLESDCLQA 256
             + +V R+S G+++ A  + +   F   ++E+ A+ +G+ FA++L   RI +ESD L  
Sbjct: 419 SSMGVVIRDSNGQVVAALCLPLQSYFSAELSEVFALEQGVLFAQELQLPRIIVESDALTV 478

Query: 257 ISLINRDAEVRNEIGTLIEDIKRRTTVFSGILF 263
           I  +N D    +  G LI+ I +    F    F
Sbjct: 479 IQAVN-DKATGSIFGHLIQGIHQVCESFESCHF 510

[Close]

BLAST of Lag0024953.1 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 5.4e-25
Identity = 80/285 (28.07%), Postives = 127/285 (44.56%), Query Frame = 0


Query: 9    SSTNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCP--NLVAET 68
            +STN     W+++W L VP K+K F+WR+ ++ IPT  NL  RGI     C       E+
Sbjct: 1123 ASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRES 1182

Query: 69   TDHNLVGCMKAREVWDLTFAH-DLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSNW 128
              H    C +AR++W   F     L  + N SF + W ++   +  +DL   AIT W  W
Sbjct: 1183 IIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIW 1242

Query: 129  ADRNKLVHNKPIPLPLIRSQLIKKYDSEYLQMN----GPLSSNSSDPY------------ 188
             DRN L+H K +     + + +  +   + Q       P + ++  P             
Sbjct: 1243 NDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSL 1302

Query: 189  ------------TGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGH 248
                        T    + R+S   L+ A+S+ +     P +AE+  I+EGLKFA     
Sbjct: 1303 KLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNF 1362

Query: 249  DRIKLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTVFSGILF 263
              +++ESD L AI LI  +   R +    + +I+  T  F+ I F
Sbjct: 1363 THLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISF 1407

[Close]

BLAST of Lag0024953.1 vs. ExPASy TrEMBL
Match: A0A6J5UN51 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28124 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-22
Identity = 83/274 (30.29%), Postives = 126/274 (45.99%), Query Frame = 0


Query: 11   TNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVA--ETTD 70
            ++S  K W +LW LKVP K+ H LWR   D++P+   L+RR I    +C    A  ETT 
Sbjct: 1423 SSSSLKSWKHLWKLKVPQKIMHLLWRVAQDILPSKEVLFRRRITQGEVCCRCFAARETTL 1482

Query: 71   HNLVGCMKAREVWD-LTFAHDLLKTKLNHSFADKWF-AMNSFISIEDLQKVAITCWSNWA 130
            H LVGC    +VW  L F  D L   L  +    W  A+ S I  +     A T W  W 
Sbjct: 1483 HALVGCDVCLQVWKALDFPSDFLLPTL--ADVGTWMDAVWSIIPPDKQSLFAFTVWVLWN 1542

Query: 131  DRN-KLVHNKPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYTGIE------------ 190
            +RN  L  ++P P  ++  Q  K YD+E+ + +     + S P   I+            
Sbjct: 1543 ERNGVLFGSQPTPSGIL-VQRAKDYDAEFKRYSAANHRSLSSPVRDIKWRPPTGNCFKLN 1602

Query: 191  ---------------IVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHD 250
                            + R+S GKL+GA ++           EL A+  G+ FA  +   
Sbjct: 1603 VDGATDMETGARGAGAIVRDSHGKLVGALAMRAPSRISVLATELYALKIGISFALDMSLV 1662

Query: 251  RIKLESDCLQAISLINRDAEVRNEIGTLIEDIKR 253
             +++ESD LQA+S++N + E     G L++ ++R
Sbjct: 1663 PLEIESDSLQAVSMVNSEEECLAAEGGLVDGVRR 1693

[Close]

BLAST of Lag0024953.1 vs. ExPASy TrEMBL
Match: A0A6J5UN24 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28123 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-22
Identity = 83/274 (30.29%), Postives = 126/274 (45.99%), Query Frame = 0


Query: 11  TNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVA--ETTD 70
           ++S  K W +LW LKVP K+ H LWR   D++P+   L+RR I    +C    A  ETT 
Sbjct: 273 SSSSLKSWKHLWKLKVPQKIMHLLWRVAQDILPSKEVLFRRRITQGEVCCRCFAARETTL 332

Query: 71  HNLVGCMKAREVWD-LTFAHDLLKTKLNHSFADKWF-AMNSFISIEDLQKVAITCWSNWA 130
           H LVGC    +VW  L F  D L   L  +    W  A+ S I  +     A T W  W 
Sbjct: 333 HALVGCDVCLQVWKALDFPSDFLLPTL--ADVGTWMDAVWSIIPPDKQSLFAFTVWVLWN 392

Query: 131 DRN-KLVHNKPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYTGIE------------ 190
           +RN  L  ++P P  ++  Q  K YD+E+ + +     + S P   I+            
Sbjct: 393 ERNGVLFGSQPTPSGIL-VQRAKDYDAEFKRYSAANHRSLSSPVRDIKWRPPTGNCFKLN 452

Query: 191 ---------------IVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHD 250
                           + R+S GKL+GA ++           EL A+  G+ FA  +   
Sbjct: 453 VDGATDMETGARGAGAIVRDSHGKLVGALAMRAPSRISVLATELYALKIGISFALDMSLV 512

Query: 251 RIKLESDCLQAISLINRDAEVRNEIGTLIEDIKR 253
            +++ESD LQA+S++N + E     G L++ ++R
Sbjct: 513 PLEIESDSLQAVSMVNSEEECLAAEGGLVDGVRR 543

[Close]

BLAST of Lag0024953.1 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 3.3e-22
Identity = 82/286 (28.67%), Postives = 132/286 (46.15%), Query Frame = 0


Query: 8   RSSTNSMAKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNL---VA 67
           +S+ +S+ + W  LW LK+P KVKHF+W+  ++ +P NVNL +RGI +SV+C      V 
Sbjct: 376 QSNEHSIVQWWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVD 435

Query: 68  ETTDHNLVGCMKAREVWDLTFAHDLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSN 127
           E+  H L  C  ++  W ++  +D LK  L          + +    E L+   +  W+ 
Sbjct: 436 ESVAHALWECKASKGYWRVSGLYDDLKQMLGEDNLTMLMRIAAEWDKEKLEFFLLVSWNI 495

Query: 128 WADRNKLVHN--KPIPLPLI-----------------RSQLIKKYDSEY-------LQMN 187
           W  RN +VH    P P  +I                 RSQ   + DS +       + +N
Sbjct: 496 WNVRNTVVHGGYHPKPEEMIEWCGNFLADFRGDTGRERSQRSSE-DSRWVPPARDQVTIN 555

Query: 188 GPLSSNSSDPYTGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHD 247
                      +G+  V R++ G ++ A++  +    PP   ELMAI +G++   Q    
Sbjct: 556 VDAGVKQGGLISGLGYVVRDAAGVVLSAAATVLQQELPPLQLELMAIKKGIQVGIQQRLQ 615

Query: 248 RIKLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTV--FSGILF 263
           R  +E+DCLQA+ LI        +I  L+  I+   +   F GI F
Sbjct: 616 RFSVETDCLQAVHLIQNKENGCRDIDGLLNHIRALISYDSFVGISF 660

[Close]

BLAST of Lag0024953.1 vs. ExPASy TrEMBL
Match: A0A803PLS0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 9.6e-22
Identity = 78/278 (28.06%), Postives = 120/278 (43.17%), Query Frame = 0


Query: 18  WSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNL---VAETTDHNLVGC 77
           W  LW LK+P KVKHF W+  ++ +P N NL +RGI + V+C      V E+  H L  C
Sbjct: 65  WKKLWRLKIPPKVKHFAWKVAHNWLPANANLAKRGIASGVVCSGCSSHVDESIAHALWEC 124

Query: 78  MKAREVWDLTFAHDLLKTKLNHSFADKWFAMNSFISIEDLQKVAITCWSNWADRNKLVHN 137
             ++  W ++  +D LK  +          + S    E L+   +  W+ W  RN +VH 
Sbjct: 125 KASKVYWMVSGFYDDLKQMMGEDNLSMLMRIASEWDKEKLEFFLLVSWNIWNVRNTVVHG 184

Query: 138 KPIPLPLIRSQLIKKYDSEYLQMNGPLSSNSSDPYT------------------------ 197
              P P    ++I+   S   +  G   S SS   T                        
Sbjct: 185 GYHPKP---EEMIEWCGSFLAEFRGDTGSRSSKCSTEDHRWVPPSMDQVTINVDAGVKQG 244

Query: 198 ----GIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHDRIKLESDC 257
               G+  V R+S G ++ A++  +    PP   ELMAI  G++   Q    R  +E+DC
Sbjct: 245 GLLSGLGYVMRDSAGAILSATATVLQQELPPLQLELMAIKRGIQAGIQRRLQRFSVETDC 304

Query: 258 LQAISLINRDAEVRNEIGTLIEDIKRRTTVFS--GILF 263
           LQA+ LI         +  L++ I+   + +S  GI F
Sbjct: 305 LQAVQLIQNKENGCRGVDGLLDQIRALISHYSFVGISF 339

[Close]

BLAST of Lag0024953.1 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-13
Identity = 69/282 (24.47%), Postives = 116/282 (41.13%), Query Frame = 0


Query: 19  SNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCP--NLVAETTDHNLVGCMK 78
           + +W+L +  K+KHFLWRAL+  + T   L  RG++    CP  +   E+ +H L  C  
Sbjct: 160 TRIWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPF 219

Query: 79  AREVWDLTFAHDLLKTKLNHSFADKWFAMNSFI---SIEDLQKV--AITCWSNWADRNKL 138
           A   W L+ +  +    +++ F +    + +F+   ++ D  K+      W  W  RN +
Sbjct: 220 ATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNV 279

Query: 139 VHNKPIPLP--------------LIRSQLIKKYDSEYLQM--------NGPLSSNSSDPY 198
           V NK    P              L  +Q  KK  S   Q+        N P +    +  
Sbjct: 280 VFNKFRESPSKTVLSAKAETHDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFD 339

Query: 199 TGIEI---------VSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLGHDRI 258
            G ++         + RN  G  I   S+ +     P  AE  A++  L+     G+ ++
Sbjct: 340 AGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQTWIRGYTQV 399

Query: 259 KLESDCLQAISLINRDAEVRNEIGTLIEDIKRRTTVFSGILF 263
            +E DC   I+LIN      + +   +EDI      F+ I F
Sbjct: 400 FMEGDCQTLINLIN-GISFHSSLANHLEDISFWANKFASIQF 440

[Close]

BLAST of Lag0024953.1 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-10
Identity = 61/275 (22.18%), Postives = 103/275 (37.45%), Query Frame = 0


Query: 21  LWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPN--LVAETTDHNLVGCMKAR 80
           +W L V  K+KHFLWR +   + TN  L  R I    +C    +  ET  H +  C   +
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 81  EVW-------------------DLTFAHDLLKTKLNHSFADK----------WFAMNSFI 140
            VW                   +L     L KT+  +S  D+          W + N F+
Sbjct: 97  SVWRSANIIIGNQWGPPSSFEDNLNRLIQLSKTQTTNSL-DRFLPFWIMWRLWKSRNVFL 156

Query: 141 SIEDLQ------KVAITCWSNWADRNKLVHNKPIPLPLIRSQLIKKYDSE-------YLQ 200
             +  Q      +  I   + W + N+   N  + +     Q  ++  S+       +++
Sbjct: 157 FQQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPEGWVK 216

Query: 201 MNGPLSSNSSDPYTGIEIVSRNSIGKLIGASSVHIDMNFPPPMAELMAIVEGLKFAEQLG 252
            N         PYT      R   G ++   +  +  +     AE +  +  L+     G
Sbjct: 217 CNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSLHAEALGFLHALQVIWAHG 276

[Close]

BLAST of Lag0024953.1 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.4e-09
Identity = 38/124 (30.65%), Postives = 58/124 (46.77%), Query Frame = 0


Query: 15  AKVWSNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNLVA--ETTDHNLV 74
           A++ + +W LK   K+KHFLW+ L+  + T  NL RR I+    C       ET+ H   
Sbjct: 12  AEIKAKIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFF 71

Query: 75  GCMKAREVWDLT-FAHDLLKTKLNHSFADKWFAMNSFISIEDLQ--KVAI-TCWSNWADR 133
            C  A++VW  +   H  L+T            ++S ++    Q   +AI   W  W  R
Sbjct: 72  DCFYAQQVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSR 131

[Close]

BLAST of Lag0024953.1 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.1e-04
Identity = 26/70 (37.14%), Postives = 34/70 (48.57%), Query Frame = 0


Query: 14 MAKVW-SNLWSLKVPAKVKHFLWRALNDVIPTNVNLYRRGIQTSVMCPNL-VAETTDHNL 73
          M   W  ++WSLK+  K+K  +W+ALN+ +P    L  R I     C      ET  H L
Sbjct: 1  MTNNWIGDIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRDFETITHIL 60

Query: 74 VGCMKA-REV 81
            C  A REV
Sbjct: 61 FNCPFAQREV 70

The following BLAST results are available for this feature:

BLAST of Lag0024953.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158377.1	1.1e-24	28.07	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_042973114.1	6.2e-23	27.72	uncharacterized protein LOC122304919 [Carya illinoinensis]	[more]
CAB4277970.1	5.2e-22	30.29	unnamed protein product [Prunus armeniaca]	[more]
CAB4277969.1	5.2e-22	30.29	unnamed protein product [Prunus armeniaca]	[more]
XP_030926547.1	1.2e-21	28.57	uncharacterized protein LOC115953156 [Quercus lobata]	[more]

BLAST of Lag0024953.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0024953.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DX30	5.4e-25	28.07	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J5UN51	2.5e-22	30.29	Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=...	[more]
A0A6J5UN24	2.5e-22	30.29	Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28123 PE=4 S...	[more]
A0A803QQT2	3.3e-22	28.67	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PLS0	9.6e-22	28.06	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lag0024953.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09510.1	1.2e-13	24.47	Ribonuclease H-like superfamily protein	[more]
AT2G02650.1	3.7e-10	22.18	Ribonuclease H-like superfamily protein	[more]
AT3G25270.1	5.4e-09	30.65	Ribonuclease H-like superfamily protein	[more]
AT3G26855.1	1.1e-04	37.14	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 9..81 e-value: 6.8E-15 score: 55.5
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 164..259 e-value: 2.1E-13 score: 50.2
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 158..263 e-value: 1.0E-5 score: 27.6
None	No IPR available	PANTHER	PTHR46736	FAMILY NOT NAMED	coord: 12..251
None	No IPR available	PANTHER	PTHR46736:SF6	SUBFAMILY NOT NAMED	coord: 12..251
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 162..253 e-value: 2.9671E-12 score: 60.0204

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0024953	Lag0024953	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0024953.1.exon2	Lag0024953.1.exon2	exon
Lag0024953.1.exon1	Lag0024953.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0024953.1	cds.Lag0024953.1_2	CDS
cds.Lag0024953.1	cds.Lag0024953.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0024953.1	Lag0024953.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity