Lag0020840.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0020840.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProteasome subunit beta
Locationchr7: 2500586 .. 2505768 (+)
Sequence length747
RNA-Seq ExpressionLag0020840.1
SyntenyLag0020840.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTCTGAAGTGAATGCTGCAGATTTTGTTTTGAAGTGCCAGGTTGTTTCTGTTTTTTTCTTACATATTGCCTAAAGTGCATTTTGAATCTTTTTCTGATGCCATCTGAAAACCTCTCATTTCTCTCCTGTTTTTTCTTGTGATAAATGCCAATGATGTCACAAGAACAGTAGGGTTTTATTTTCAACAACCAATGGATGAGCTTCCATGTGACCATCCTTAGCTTTTTTTAATTATCTTGAAAATTGAAAGTTTTGAATTATAATTTTAGTTTGAACCACCATGGATTAGTTGAGTGGTTGATGAGAACCATAGAAAAAATAAAGGGTTAAGTTCAATCCTTGATGGTCACATACTTGGAATTTAATATCCTACAACGATCAAATGTGAGATAGGCGATTGTTCCATCTCGTTAAATTAGTTGAGGTGTGTGCAACCTGACTCGAATATTCACGGATATATATATAAGAAAAAACTATAATTTTAGTTTGTATTAATTTCAATTGAGTGTTTATGCTATAAAAAGTTTTATCTATTTCCTTCAATTAACTTTTATTATTCTTGGCTTCAATGAAAAGTAACAAAAATTCTACTTGGTAAGCTGTTTGTGTCTGTCTGCATGCTAGGATAAATAAGAGTAAAATCTATTGGTCATTATTGATTTCACTTTAAAATATAATTTTTTAAAATTTATTTCTACTTTCAAAATAATGTCTTAATATTCGATTCAAATTATGTTTTTTAAAAAATAATATGACTTATTACGTCACATCATTAAAGCAAAATATTAGATAACAAAACTTAAAATATCACCTTTACGAACAATACTATAATACACGGAATTATTGTACTCTTCACCAATAAACATAACTGATGGTAAGTTTTAGCCAAAATGATAGAGCAACTGTAAAACTAATTCTAAGAAATGAAGATTAAAAATGTGAGGTTAAAATATTATTTTGGTCTTTGTACTATATGATTGTTCTATTTTTTTTTTGAAAAATATGACTTGTTCTATTTTAGTTCTTATACTTTCAAATGTTGAAATTTACTATTGATACTTTCCATAAATCTTAAATGTAGTCTAGTGTTTTTGTTTTCCTAAAAAAATTGTTTTTTTTTTTTTTTTTTTTTTTTGTCTCCATTAAGTTTATTTTTATAAATTTTGGAAACATATTTATATTGTGTCTTTGATTGAATAAAATAATAATAATTATTGTTCCAAGTTTGATGGAAACAAACTTCAAACTAACCATGGAGACTAATATTAAGATTTATAGAAAATATACAAACTAAATTTGGATATTTGAAAATAGGTTTAGATTTAATGAGATTTCTCACATAGATAAATTAATAAACTAATTTGGGAACCAATAGTTTTATAAGGAACAAAGTCTAATTATTAAATAATAAAAACCAACATTTAATTTTGATGTTTTTTTTTTTTGTGACAAGGATTAAATTATTAAGAATTTGAAAGTACATGAACTAAATTGTTACAAAATTGAAAGTACATGGACTAGATCGTTACAAATCAAAGTTCAGGAACTAAATTGTCACTTTTATGAAAGCTCAGGGGACTAAAAGCGATTTTTAACCTTGAAAATACGACTAAAATAAAATGAGTCTCATATTACAGTACCACAATGATATTTTAACCAAAATGTAAATTAACAACGAGAAAAATACTTTCCAACTAATGAAATAAAAATTGATATGTCATTCTATCCACCTCAACACATATCAATATTTCGTTAGGTTAAAGTTAAATAAAGGACTATTTAAAATCATTTTGCAAACTTGGAGGACTAAAATGTCATTTTTGATTTGAGTCTCAAATCAAAAGTGCATTTTGCACCCAAAAAAAATTATTTGGGGATTTTATTTTATTTTTTTTTTCATTTAAAAAAAAATCCTCAATTTTACACAAAACCCTCTATCCAAGCCTGAAGAAAATTGGGCTTTATATTGTGCAATTTTACACAAATTCATTGGAACAGCCCATCCGTATAAATAGATTGGGCCGATCAAAACGCTGTCGTTTATTCCTTCAATTCCTTCCCATTTTTCTTGAGCGAAAAGACACCGTGGGATCTAAAGAAAAGAAGCGAGCAAGCTTAGAAAAACCAGAGCAAGTTTCTTGCCTTTGCTTTGCTAAGTTCCTCTTCTCTCAGACGCCTTCGCTTTTATCTGCCCTATTCGCCATGACGAAGCAGCAAGCCAACTGGTCCCCTTACGACAACAATGGCGGGTACTTTCTCTCTCTCTCAATCTCTCTGCAGCGTTTCGATGTTTCTTTTATCTGTTACGGTGACGAATCAGTGAGAAACTTTGTGCAGATCCTGTGTCGCGATTGCCGGTGCCGATTACTGTGTAATCGCCGCTGATACTCGGTTGTCTACGGGTTACAATATCCTCACTCGCGATTACTCCAAAATTTGTAAGCTGTACGTCAATCTCTTATCGAATTTAGCTTTATTTTGGAATTTGAATGCTTAAGGTCACTCGAGTTTCAGAGCGGCTTTCCGAATTATGTGGTTTGGTGTTTATTTTATTTTCTATGTGTATTAGAAATGGATCCTTTCCCCCTTTGTAGTGTTTTTTTTTACTCCTGGAAATTGTATTGCGCTTCGGGTTTATTTATGTTTTTGTGGCTATTACTTGGGGGTAGACTTGTGGATTTTAGAGTTCTCTCAGGGTTATAGTTGGGTGGGCTTTTGAACGAAATGAGCGGTAACTTATTTTGAAGATTGTGCATTGGTGTGTAACAATGGCTGGATTAGCTTGTGAATGTGATTGGTGCCAGCTTACTTGAACTATGGTGCTGTGCAAATTGGAAATTTGGTCATTTCGTGCTTTTGTTTGGAAGGTTTTGGTTTTGCCTACGAAGGGTTATGGATCCGTTAGTGGTATCGACGAAAGGTTATGAAAGTAGTAATGGAGGTTTATTCTTTTAAGATTGATGGATCCGTTGGATGCTGAGTTTATCACTTGTGATTTTTGTGTTTATTGATACCTGTTGGTTAATGAGTTGGGAGATAAATTATCTGTACATCCTAACTTTAGTTCAAGGAGCTAATTGGTCTATGATACTCCTTTTTGTGAATGTGAAAGAGAGCCTCCTTTGTTTTGATAGAAAGGAAAGAAACAATCATTTCTCAACTTTTGGTTGTGTTTTTGAATTCTATTGTACTGAGATATGACTAGATTTAGAATGTAATTCAATTGGTCGAATGGTATGTATAATGTATGCGATTGTTATATGATTTCCTCAATTTGGTCATGGCAGATTTCCTCGTGTTTTGTTATTGAACTTCATCTAATATGGATTCTCGATCATGCTAATTCCATGACAAGAGTTCCATATAATGCATTTTTGGTTCTATTGTTGTGATGATAATAATATACTTTACTCACCTTAAAGCTCATTCATCCACACACGTCTTACATTGCACTGATGTGCTTATTTATGATTCTTGATGTTGCAGGGCTGACAAAGCTGTGATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCCTTACAAAAGGTTTTGGCGGCTAGGCATTTGGTGAGACTTTTATCTAAATGAATATGTCTAATTAGGTTGATCTCTGTAATCCATATTAGCTAAATGCTATGCTATAAGCTTCGTTTTCAAGACTTTGAGTTTTTGATGCACCTAATTGTTCTCATTTTCTGTGAGTTCGATATTGTGTGGGTTACTAATTTCTACCTGAAGATTTATCCCTTTAATGAGACTCCTTTTTTTTTTTTTTTTTTTGATTTCGATATCTGTTTTTGTAGGTGAGCTTGTATCAGGCTTGGATTATTGTATATTATTGTTGGTGCTGCATTCCTTAAAATTAGGATAGTATGGTTTTAAGATTTCCAACACCATGCTTCCCAAGTGACATTTTGTTCCTAGCCTTTTGTTTATAGTTGAGACATTCACTGGTGTTTTTGATGTCACAATGATGTTTTGATCTTATAACGATTTTTGGAGGGAAGTGACTATAACTTTCCTTTTTGTGATTTTCTATACTGTAATCTTTAATTTTTTTTTAACAAGTTTTGCGTACATCTTGATATTTGAGCCCTTTTCCCTTTTGGGCTTCTGATCCCATCCCAGTCTTTCATGCATAAACTAATTTTGTTCGTAGTGGAACTCTATTTTTCTTTTGAAATGTATTACAAGGATTGCCTTCAAAAGTTGAATTGAACTATGTGCCGAGGGAAGCAGATGAACAAAACGTCTTGTCAATTTCAGTTTTTAAATTTATTAGGTATTTTAGACGTAAGATTTTGGGTCGAAGGATTGGAATATGATTCATTATTTTGATGCTGCATATGTTGTTCCATTTCAGACCTACCAACATCAGCACAACAAGCAAATGAGCTGCCCCGCGATGGCTCAACTGCTCTCAAATACATTGTACTTCAAACGGTTCTTTCCTTATTATGCTTTTAACGTTTTGGGCGGACTTGATAACGAAGGTACATGACTTCCCTGATTGTAATTCCAGTTTTATGCCTCACTTTCCCATTAACTATAAGTTATTCTCTTCTTGAAATAGGAAAGGGTTGTGTGTTCACCTATGATGCTGTTGGTTCTTATGAGAGAGTTGGATACAGCGCTCAGGGGTCGGGGTCTACCCTCATTACGCCCTTTTTGGATAACCAACTGAAGTCTCCCAGCCCTCTATTATTGCCTGCACAGGTATGACCTTACCCTTCCTTGGGAGGAATGTAAATGGAAATGACATGTCGGTTCAAGAAACGCGAGGTTTACTTTGCCTATCTGATAGTTCAAAGTTGAAACCAACCGTTCCAACTCGGTCCGGTTCACTCTGTTGTTTATCCTGTTTTGCCGTGCAATACTCTTTCCTCCTCTTTGGTATATCTCTCTCTCAGCTAACAAATTATGATGAACAGGATTCTGCAACGCCTCTTTCAGAAGCCGAAGCAATTGACCTGGTGAAAACCGTTTTTGCATCTGCTACTGAGAGGGATATCTACACTGTAATTTTCTTCCACTATTTATGTACCCTCATTCTGAATAAGTATTTGTTTGGATTCCAACTCGAAATTTTGTGTTATTGACAGGGTGACAAACTCGAAATCGTCATTCTTAATGCCGATGGTATTCGTCGTGAATACATGGATCTCCGAAAAGATTAG

mRNA sequence

ATGGTGTCTGAAGTGAATGCTGCAGATTTTGTTTTGAAGTGCCAGACGCCTTCGCTTTTATCTGCCCTATTCGCCATGACGAAGCAGCAAGCCAACTGGTCCCCTTACGACAACAATGGCGGATCCTGTGTCGCGATTGCCGGTGCCGATTACTGTGTAATCGCCGCTGATACTCGGTTGTCTACGGGTTACAATATCCTCACTCGCGATTACTCCAAAATTTGTAAGCTGGCTGACAAAGCTGTGATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCCTTACAAAAGGTTTTGGCGGCTAGGCATTTGACCTACCAACATCAGCACAACAAGCAAATGAGCTGCCCCGCGATGGCTCAACTGCTCTCAAATACATTGTACTTCAAACGGTTCTTTCCTTATTATGCTTTTAACGTTTTGGGCGGACTTGATAACGAAGGAAAGGGTTGTGTGTTCACCTATGATGCTGTTGGTTCTTATGAGAGAGTTGGATACAGCGCTCAGGGGTCGGGGTCTACCCTCATTACGCCCTTTTTGGATAACCAACTGAAGTCTCCCAGCCCTCTATTATTGCCTGCACAGGATTCTGCAACGCCTCTTTCAGAAGCCGAAGCAATTGACCTGGTGAAAACCGTTTTTGCATCTGCTACTGAGAGGGATATCTACACTGGTGACAAACTCGAAATCGTCATTCTTAATGCCGATGGTATTCGTCGTGAATACATGGATCTCCGAAAAGATTAG

Coding sequence (CDS)

ATGGTGTCTGAAGTGAATGCTGCAGATTTTGTTTTGAAGTGCCAGACGCCTTCGCTTTTATCTGCCCTATTCGCCATGACGAAGCAGCAAGCCAACTGGTCCCCTTACGACAACAATGGCGGATCCTGTGTCGCGATTGCCGGTGCCGATTACTGTGTAATCGCCGCTGATACTCGGTTGTCTACGGGTTACAATATCCTCACTCGCGATTACTCCAAAATTTGTAAGCTGGCTGACAAAGCTGTGATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCCTTACAAAAGGTTTTGGCGGCTAGGCATTTGACCTACCAACATCAGCACAACAAGCAAATGAGCTGCCCCGCGATGGCTCAACTGCTCTCAAATACATTGTACTTCAAACGGTTCTTTCCTTATTATGCTTTTAACGTTTTGGGCGGACTTGATAACGAAGGAAAGGGTTGTGTGTTCACCTATGATGCTGTTGGTTCTTATGAGAGAGTTGGATACAGCGCTCAGGGGTCGGGGTCTACCCTCATTACGCCCTTTTTGGATAACCAACTGAAGTCTCCCAGCCCTCTATTATTGCCTGCACAGGATTCTGCAACGCCTCTTTCAGAAGCCGAAGCAATTGACCTGGTGAAAACCGTTTTTGCATCTGCTACTGAGAGGGATATCTACACTGGTGACAAACTCGAAATCGTCATTCTTAATGCCGATGGTATTCGTCGTGAATACATGGATCTCCGAAAAGATTAG

Protein sequence

MVSEVNAADFVLKCQTPSLLSALFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD
Homology
BLAST of Lag0020840.1 vs. NCBI nr
Match: KAG6582562.1 (Proteasome subunit beta type-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.5 bits (1186), Expect = 4.7e-126
Identity = 231/234 (98.72%), Postives = 233/234 (99.57%), Query Frame = 0

Query: 15  QTPSLLSALFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI 74
           +TPSLLS LFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI
Sbjct: 52  KTPSLLSDLFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI 111

Query: 75  CKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP 134
           CKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP
Sbjct: 112 CKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP 171

Query: 135 YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA 194
           YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA
Sbjct: 172 YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA 231

Query: 195 QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 232 QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 285

BLAST of Lag0020840.1 vs. NCBI nr
Match: KAG6597195.1 (Proteasome subunit beta type-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 456.8 bits (1174), Expect = 1.2e-124
Identity = 228/234 (97.44%), Postives = 231/234 (98.72%), Query Frame = 0

Query: 15  QTPSLLSALFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI 74
           +TPSL S LFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI
Sbjct: 360 KTPSLFSDLFAMTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKI 419

Query: 75  CKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP 134
           CKLADKAVMASSGFQADVKALQKVL+ARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP
Sbjct: 420 CKLADKAVMASSGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP 479

Query: 135 YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA 194
           YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA
Sbjct: 480 YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA 539

Query: 195 QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIR EY+DLRKD
Sbjct: 540 QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRHEYLDLRKD 593

BLAST of Lag0020840.1 vs. NCBI nr
Match: XP_022924840.1 (proteasome subunit beta type-1 [Cucurbita moschata] >XP_022980398.1 proteasome subunit beta type-1 [Cucurbita maxima] >XP_023527434.1 proteasome subunit beta type-1 [Cucurbita pepo subsp. pepo] >KAG7018944.1 Proteasome subunit beta type-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445.7 bits (1145), Expect = 2.7e-121
Identity = 222/223 (99.55%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. NCBI nr
Match: XP_038898567.1 (proteasome subunit beta type-1 [Benincasa hispida])

HSP 1 Score: 444.9 bits (1143), Expect = 4.6e-121
Identity = 221/223 (99.10%), Postives = 222/223 (99.55%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSE E
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEGE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIV+LNADGIRREYMDLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVVLNADGIRREYMDLRKD 223

BLAST of Lag0020840.1 vs. NCBI nr
Match: XP_022942711.1 (proteasome subunit beta type-1-like [Cucurbita moschata] >XP_022974164.1 proteasome subunit beta type-1-like [Cucurbita maxima])

HSP 1 Score: 444.5 bits (1142), Expect = 6.0e-121
Identity = 221/223 (99.10%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL+ARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy Swiss-Prot
Match: O82531 (Proteasome subunit beta type-1 OS=Petunia hybrida OX=4102 GN=PBF1 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 4.3e-114
Identity = 202/223 (90.58%), Postives = 214/223 (95.96%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGG+CVA+AGADYCVIAADTR+STGYNILTRDYSKI KLADK VMAS
Sbjct: 1   MTKQQANWSPYDNNGGTCVAVAGADYCVIAADTRMSTGYNILTRDYSKIIKLADKCVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADV+ALQKVLA+RHL YQHQHNKQMSCPAM QLLSNTLY+KRFFPYY+FNVLGGLD
Sbjct: 61  SGFQADVRALQKVLASRHLIYQHQHNKQMSCPAMGQLLSNTLYYKRFFPYYSFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           +EGKGCVFTYDAVGSYERVGYS+QGSGSTLI PFLDNQLKSPSPLLLPAQD+ TPLSEAE
Sbjct: 121 SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKT FASATERDIYTGD+LEIVILNA GIRRE M+LRKD
Sbjct: 181 AIDLVKTCFASATERDIYTGDRLEIVILNASGIRREEMELRKD 223

BLAST of Lag0020840.1 vs. ExPASy Swiss-Prot
Match: P42742 (Proteasome subunit beta type-1 OS=Arabidopsis thaliana OX=3702 GN=PBF1 PE=1 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 1.2e-111
Identity = 199/223 (89.24%), Postives = 211/223 (94.62%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQ ANWSPYDNNGG+CVAIAG+DYCVIAADTR+STGY+IL+RDYSKI KLAD+AV++S
Sbjct: 1   MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
            EGKGCVFTYDAVGSYERVGY AQGSGSTLI PFLDNQLKSPSPLLLP QDS TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           A+DLVKTVFASATERDIYTGDKLEI+IL ADGI+ E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy Swiss-Prot
Match: O64464 (Proteasome subunit beta type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PBF1 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 2.7e-100
Identity = 177/213 (83.10%), Postives = 195/213 (91.55%), Query Frame = 0

Query: 36  YDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMASSGFQADVKAL 95
           Y+NNGG+CVAIAGADYCV+AADTRLS GYNILTRD+SKIC+LADK  +ASSGFQ D+KAL
Sbjct: 9   YENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKAL 68

Query: 96  QKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDNEGKGCVFTY 155
            K LAAR L YQHQHNK+MSCPAMAQLLSNTLY+KRFFPYYAFNVLGGLD+EGKGCVFTY
Sbjct: 69  HKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFTY 128

Query: 156 DAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAEAIDLVKTVFA 215
           DAVGSYER GYSAQG+GS LI P LDNQLKSPSPLLLPA+D+ TPLSE EA+DLVK VFA
Sbjct: 129 DAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDVFA 188

Query: 216 SATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           SATERDIYTGDKLEIV++N  G +REY+DLRKD
Sbjct: 189 SATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221

BLAST of Lag0020840.1 vs. ExPASy Swiss-Prot
Match: Q86A21 (Proteasome subunit beta type-1 OS=Dictyostelium discoideum OX=44689 GN=psmB1 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-53
Identity = 114/234 (48.72%), Postives = 158/234 (67.52%), Query Frame = 0

Query: 20  LSALFAMTKQQ----ANWSPYDNNGGSCVAIAGADYCVIAADTRLST-GYNILTRDYSKI 79
           LSAL    KQQ      +  Y +NGG+ +A+AG DYCVIA DTR+S  GY I TR Y+KI
Sbjct: 6   LSALPEKDKQQPISHGRFEAYVDNGGTVLAVAGKDYCVIAGDTRMSDGGYGIQTRKYTKI 65

Query: 80  CKLADKAVMASSGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFP 139
            +L  K V+A+SG QAD  ALQK L +   +++ +H K MS PA+AQ LSNTLY+KRFFP
Sbjct: 66  FQLTKKCVLATSGMQADTIALQKRLKSMLESFEKEHGKPMSTPAIAQFLSNTLYYKRFFP 125

Query: 140 YYAFNVLGGLDNEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPA 199
           YY FN++ G+D +G+G ++TYDAVGS+ERV YS+QGSG+ L+ P LDNQ+   +  ++  
Sbjct: 126 YYTFNLVAGVDEQGEGWIWTYDAVGSHERVKYSSQGSGNQLVIPLLDNQVAKFNQQIV-- 185

Query: 200 QDSATPLSEAEAIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
            +    +S  + +  VK    SA ERDIYTGD  +IV+++ +G+  E  +L+ D
Sbjct: 186 -EGNKDVSCEQVVSFVKDSITSAGERDIYTGDFADIVVVDKNGVHWEKFELKLD 236

BLAST of Lag0020840.1 vs. ExPASy Swiss-Prot
Match: Q9IB84 (Proteasome subunit beta type-1-A OS=Carassius auratus OX=7957 GN=psmb1-A PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 5.5e-53
Identity = 105/216 (48.61%), Postives = 144/216 (66.67%), Query Frame = 0

Query: 33  WSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMASSGFQADV 92
           +SPY  NGG+ +A+AG D+ ++A+DTRLS GY+I +RD  K  KL D  V+  SGF  D 
Sbjct: 27  FSPYAFNGGTVLAVAGEDFALVASDTRLSEGYSIHSRDSPKCYKLTDTTVIGCSGFHGDC 86

Query: 93  KALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDNEGKGCV 152
             L K++ AR   Y+H +NK M+  A+A +LS  LY +RFFPYY +N++GGLD EG+G V
Sbjct: 87  LTLTKIIEARLKMYKHSNNKSMTSGAIAAMLSTILYGRRFFPYYVYNIIGGLDEEGRGAV 146

Query: 153 FTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAEAIDLVKT 212
           +++D VGSY+R  Y A GS S ++ P LDNQ+   +           PLS+ +A+ LVK 
Sbjct: 147 YSFDPVGSYQRDTYKAGGSASAMLQPLLDNQIGFKN----MENVEQVPLSQEKAVQLVKD 206

Query: 213 VFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           VF SA ERD+YTGD L+I I+  +GIR E + LRKD
Sbjct: 207 VFISAAERDVYTGDALKICIITKEGIREEIVPLRKD 238

BLAST of Lag0020840.1 vs. ExPASy TrEMBL
Match: A0A6J1IZ68 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111479776 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 222/223 (99.55%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy TrEMBL
Match: A0A6J1EG74 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111432207 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 222/223 (99.55%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy TrEMBL
Match: A0A6J1IGT1 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111472752 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.9e-121
Identity = 221/223 (99.10%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL+ARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy TrEMBL
Match: A0A6J1FPP2 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111447663 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.9e-121
Identity = 221/223 (99.10%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL+ARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE
Sbjct: 121 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREY+DLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYLDLRKD 223

BLAST of Lag0020840.1 vs. ExPASy TrEMBL
Match: A0A5D3D235 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003970 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.4e-120
Identity = 218/223 (97.76%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS
Sbjct: 1   MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL+ARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
           +EGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDS+TPLSEAE
Sbjct: 121 SEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSSTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           AIDLVKTVFASATERDIYTGDKLEIV+LNADG+RREYMDLRKD
Sbjct: 181 AIDLVKTVFASATERDIYTGDKLEIVVLNADGLRREYMDLRKD 223

BLAST of Lag0020840.1 vs. TAIR 10
Match: AT3G60820.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 8.3e-113
Identity = 199/223 (89.24%), Postives = 211/223 (94.62%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQ ANWSPYDNNGG+CVAIAG+DYCVIAADTR+STGY+IL+RDYSKI KLAD+AV++S
Sbjct: 1   MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
            EGKGCVFTYDAVGSYERVGY AQGSGSTLI PFLDNQLKSPSPLLLP QDS TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           A+DLVKTVFASATERDIYTGDKLEI+IL ADGI+ E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223

BLAST of Lag0020840.1 vs. TAIR 10
Match: AT3G60820.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 8.3e-113
Identity = 199/223 (89.24%), Postives = 211/223 (94.62%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQ ANWSPYDNNGG+CVAIAG+DYCVIAADTR+STGY+IL+RDYSKI KLAD+AV++S
Sbjct: 1   MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
            EGKGCVFTYDAVGSYERVGY AQGSGSTLI PFLDNQLKSPSPLLLP QDS TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           A+DLVKTVFASATERDIYTGDKLEI+IL ADGI+ E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223

BLAST of Lag0020840.1 vs. TAIR 10
Match: AT3G60820.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 3.5e-111
Identity = 197/223 (88.34%), Postives = 210/223 (94.17%), Query Frame = 0

Query: 26  MTKQQANWSPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMAS 85
           MTKQ ANWSPYDNNGG+CVAIAG+DYCVIAADTR+STGY+IL+RDYSKI KLAD+AV++S
Sbjct: 1   MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60

Query: 86  SGFQADVKALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 145
           SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD
Sbjct: 61  SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120

Query: 146 NEGKGCVFTYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAE 205
            EGKGCVFTYDAVGSYERVGY AQGSGSTLI PFLDNQLKSPSPLLLP QDS TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180

Query: 206 AIDLVKTVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
           A+DLVKTVFASATERDIYT +KLEI+IL ADGI+ E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTVNKLEIMILKADGIKTELMDLRKD 223

BLAST of Lag0020840.1 vs. TAIR 10
Match: AT1G21720.1 (proteasome beta subunit C1 )

HSP 1 Score: 80.1 bits (196), Expect = 2.8e-15
Identity = 61/216 (28.24%), Postives = 98/216 (45.37%), Query Frame = 0

Query: 34  SPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMASSGFQADVK 93
           S ++ NG + VA+ G +   IA+D RL      +  D+ +I K+ D+  +  SG   DV+
Sbjct: 2   SIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDVQ 61

Query: 94  ALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDNEGKGCVF 153
            L + L  RH  YQ +  + M     A L+S  LY KRF PY    V+ GL ++ K  + 
Sbjct: 62  TLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGDDDKPFIC 121

Query: 154 TYDAVGSYERVGYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAEAI-DLVKT 213
           T D++G+ E            L   F+ +   S S L    +    P  EAE + + +  
Sbjct: 122 TMDSIGAKE------------LAKDFVVSGTASES-LYGACEAMYKPDMEAEELFETISQ 181

Query: 214 VFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
              S+ +RD  +G    + I+    I+   +  R D
Sbjct: 182 ALLSSVDRDCLSGWGGHVYIVTPTEIKERILKGRMD 204

BLAST of Lag0020840.1 vs. TAIR 10
Match: AT1G77440.1 (20S proteasome beta subunit C2 )

HSP 1 Score: 78.2 bits (191), Expect = 1.0e-14
Identity = 56/217 (25.81%), Postives = 96/217 (44.24%), Query Frame = 0

Query: 34  SPYDNNGGSCVAIAGADYCVIAADTRLSTGYNILTRDYSKICKLADKAVMASSGFQADVK 93
           S ++ NG + VA+ G +   IA+D RL      +  D+ +I K+ D   +  SG   DV+
Sbjct: 2   SIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDHLFIGLSGLATDVQ 61

Query: 94  ALQKVLAARHLTYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDNEGKGCVF 153
            L + L  RH  YQ +  + M     A L+S  LY KRF P+    V+ GL ++ K  + 
Sbjct: 62  TLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIAGLGDDNKPFIC 121

Query: 154 TYDAVGSYERV-GYSAQGSGSTLITPFLDNQLKSPSPLLLPAQDSATPLSEAEAI-DLVK 213
           T D++G+ E    +   G+ S  +    +   K              P  EAE + + + 
Sbjct: 122 TMDSIGAKELAKDFVVSGTASESLYGACEAMFK--------------PDMEAEELFETIS 181

Query: 214 TVFASATERDIYTGDKLEIVILNADGIRREYMDLRKD 249
               S+ +RD  +G    + ++    ++   +  R D
Sbjct: 182 QALLSSVDRDCLSGWGGHVYVVTPKEVKERILKGRMD 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6582562.14.7e-12698.72Proteasome subunit beta type-1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6597195.11.2e-12497.44Proteasome subunit beta type-1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022924840.12.7e-12199.55proteasome subunit beta type-1 [Cucurbita moschata] >XP_022980398.1 proteasome s... [more]
XP_038898567.14.6e-12199.10proteasome subunit beta type-1 [Benincasa hispida][more]
XP_022942711.16.0e-12199.10proteasome subunit beta type-1-like [Cucurbita moschata] >XP_022974164.1 proteas... [more]
Match NameE-valueIdentityDescription
O825314.3e-11490.58Proteasome subunit beta type-1 OS=Petunia hybrida OX=4102 GN=PBF1 PE=2 SV=1[more]
P427421.2e-11189.24Proteasome subunit beta type-1 OS=Arabidopsis thaliana OX=3702 GN=PBF1 PE=1 SV=2[more]
O644642.7e-10083.10Proteasome subunit beta type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PBF1 ... [more]
Q86A211.1e-5348.72Proteasome subunit beta type-1 OS=Dictyostelium discoideum OX=44689 GN=psmB1 PE=... [more]
Q9IB845.5e-5348.61Proteasome subunit beta type-1-A OS=Carassius auratus OX=7957 GN=psmb1-A PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1IZ681.3e-12199.55Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111479776 PE=3 SV=1[more]
A0A6J1EG741.3e-12199.55Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111432207 PE=3 SV=1[more]
A0A6J1IGT12.9e-12199.10Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111472752 PE=3 SV=1[more]
A0A6J1FPP22.9e-12199.10Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111447663 PE=3 SV=1[more]
A0A5D3D2352.4e-12097.76Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT3G60820.18.3e-11389.24N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G60820.38.3e-11389.24N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G60820.23.5e-11188.34N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT1G21720.12.8e-1528.24proteasome beta subunit C1 [more]
AT1G77440.11.0e-1425.8120S proteasome beta subunit C2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 33..248
e-value: 1.1E-63
score: 216.7
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 34..247
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 38..232
e-value: 1.8E-34
score: 118.9
NoneNo IPR availablePANTHERPTHR11599:SF170PROTEASOME SUBUNIT BETAcoord: 29..248
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 29..248
NoneNo IPR availableCDDcd03757proteasome_beta_type_1coord: 33..248
e-value: 6.73137E-132
score: 368.895
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 44..91
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 40..233
score: 48.276821

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0020840Lag0020840gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0020840.1.exon1Lag0020840.1.exon1exon
Lag0020840.1.exon2Lag0020840.1.exon2exon
Lag0020840.1.exon3Lag0020840.1.exon3exon
Lag0020840.1.exon4Lag0020840.1.exon4exon
Lag0020840.1.exon5Lag0020840.1.exon5exon
Lag0020840.1.exon6Lag0020840.1.exon6exon
Lag0020840.1.exon7Lag0020840.1.exon7exon
Lag0020840.1.exon8Lag0020840.1.exon8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0020840.1cds.Lag0020840.1CDS
cds.Lag0020840.1cds.Lag0020840.1_2CDS
cds.Lag0020840.1cds.Lag0020840.1_3CDS
cds.Lag0020840.1cds.Lag0020840.1_4CDS
cds.Lag0020840.1cds.Lag0020840.1_5CDS
cds.Lag0020840.1cds.Lag0020840.1_6CDS
cds.Lag0020840.1cds.Lag0020840.1_7CDS
cds.Lag0020840.1cds.Lag0020840.1_8CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0020840.1Lag0020840.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0050832 defense response to fungus
biological_process GO:0010498 proteasomal protein catabolic process
cellular_component GO:0005839 proteasome core complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
molecular_function GO:0004298 threonine-type endopeptidase activity