Lag0020729.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0020729.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr7: 1675423 .. 1679443 (-)
Sequence length1476
RNA-Seq ExpressionLag0020729.1
SyntenyLag0020729.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAATGGGGAAACTCTACAAAAACCCCATTAAAGCTCAAGTAAGCAAAAAAAAAAAAAAAAAAAAAAAAGCTTTTTCTTCCTTCTCAAATGGCTTCCTTCATTTCAATCTCTTCAAAAAAGCTTCCATTTTTAATGGCTTTTGGGTTCAAATCCACTGACCCATTTACCCTTTTTAGCATTTGAACAAACCCTTTTGATCTAAATGCTTCTGTTTTCTTAATGAAAAATGTTGTTGTTCTTTTTACAGGTGGTGAAAATGACGATTTTGGTGCTCGTTCTGGCGGCGGCGTTGATGGGGGAAGTTGAGGGAAGGAAGGGCAAAAAGGTAAAAAAGGCGAGGATTTTGGAGGGCAATGGGATTGAATATGGGGCCATAAGTTGCAGAGCTCACAGTGCTTCGATAACGGAGTTCGGCGGCGTTGGCGACGGCGAGACGTCGAATACGAAGGCGTTTCAGACGGCGGTGGAGCTGCTGAGCCAGTACGCCGGAGACGGCGGCGCTCAGCTGTTTGTGCCGGCGGGGAGGTGGCTCACGGGGAGTTTCAATCTGACCAGTCATTTCACTCTGTTTCTGCACCGAGATGCTGTCCTTCTTGCTTCTCAGGTTTCACTTTTTTCACCTTTCTCTCTCAAATTTAAATATATTTTATCTTATTCATATGAAATGTTGTCTTGTACTCTTGTCTTTTCATTTTATATAAATTTTTATTATTATATTTTTAAAATTAAATCTCAGTTTTTGTATTTTTAATGTTAAAAACCATTTTTTTTTCAAGAAGATTGAATTGTTACAAATCAAAATCTACGAATTAAATTAAATTGAGATTCAAATGTTTCTTTGAAATCTTAGTAAATGGTCATCGTGAATTGAACTCATTTATGTGAACGTTTTATTTTTTTCATGATCCTTATTGATCACTAGATCAAGATGGTTGCTTTATGAATGTATTATTATAAGCTAAATTGTTCATTTTTTTAAAGCAAAATAACAATAAGGAACAAAATTATTTTTAACAAAAATGTTTTAAAGTTATTTAAAATATTCATAGATGAAAATGGGATTTGAATTTTTTAAAATTTCTTTGTTAATTTTTTTTTTATATCGATACACTATAGAATTTTTGCATGAGTGCTATTTTAAAATGCATATATTAATTATTCTATCTCTTAAGAAAATGGATGAACTAATATATATATATATATATATATTTCTTGAGAAAATGAAGTAATATGTTTGTTACATGGAATATATAAATTAATATTAGTTCACGAGTGACCTATTACCAGATCCAATTTCATCGAAATATTTACATTTGCAGCATCACGTGAATTGATGGCGTCATGTTTGTTTCATATCCAATTTCTTTTTTTTTTTTTTTTATATTTATAAAATGAAAATATTTTGAATAAGATTTTATATAACATTGAATTCCATTTGTAATAATTATATAAGAATGATAGAAATGCCCAAATTTTCTTTTTCTTTCACAAATCATTCCATTTCATGTGAAGACAATTATAGCGGTTGAGAAAGATGAGTATGCGTTACAAAATTACATATATAAATTTTAATGCTTGTCAACATTTATAGGAATCTTCTTGTGGAATATTTAGGTAACTTCCAACCACATTTTAGCATCTTTTATAGTAGAAACTGCCAGCTTATCAGCTTTCATTTTTCCTTTTTAACCACTCTTCTTAATGTTACCACGTGCACTACAAATAGGAAATAAATAGAAAATGACATTGTGGTACTCAATTGGTTAAGTCACATACTAGACCAGGAGGTCGAAGTTCAAATCCTTCAACCCAAATATGTTGAATAAAAGTAAACGACTTGAATTGAAGACCTTCGAGTCAATAACATATTAAGTCATCGCGGGCTTAAAAATGTTTAGTATAAGTTTGGATGAGTTTAATATTCTTTAATGTACTATCAACATCTTTTTCTTTGTAGACAAACATATGGTCATATTTCATGACTTTTGAAGAGATGATAATTAAGTTCATTTAATCATAATTTAATGACTCTAGAAGCAAAGATTCAAATGGAAAAGAGTTCTCAAAGATGAGAGTTATTATTAGGGTAGATGGGGGCAATTTTGATGTTATTATATCTTAGAGGACATTTATCAGCCAAAAGAAAAAAGAAAAAGAAAAAGAAACTAAGTTTGGGTAGTTAGAATTAGGCAATTTAGAATCAATTTATCATAATCAAAGTGGTTCTCTTTTTGAATGTTTGGTTTTACAAAGTCATCAAGGCAAAGACATTGGTATCTTGACTTTAACAAATTGAAGTGATGAGTCAAAAGTGGGGGTAGTTTCTTCAAAACAAACTACCAAAAATTGGTTGTCTGAAATCCATTGGATTCCATGTGAATTAGGTCCTAAAATCATGCCCTTCCATTTTCCAAACCCTAGGTGTGGTTAGGTCAGCTGAAGTTGTTAAAAGATGGGAAATGAGGATGTTGAACTAACTCTAAGGTCAAGCTCCATCCCCACATAACAAAACCATTAGTCTTAGGTTAGCCATTCATTTCATATAGATATTGGTACCGAGAAAATCTTAAATCAACCTAACTTTTCAATCCAACGATCCAAAATAGATCATGTAAATTTGATCAGTTGATGTGCCTTTGAGTCATTTCATCTAAAACTTGACTGATTTTCAACTATTTCGATAGGATCCGAGTGAATGGCCTGTGGTCGAACCGTTGCCATCCTACGGTCATGGAAGGGACACATCAGGGGGAAGATATATCAGTTTAATATTTGGAACAAACCTCACTGATGTTGTCATCACAGGTAATGTGTTCCACTGCTAGAGCTTGTCTCTCATTTTTCTTGATTGGTGTCTGATAATTCGAATGGAGCCTGATCGACGATCGACAACAGGAAACAACGGCACCATTGATGGTCAGGGTGATCGCTGGTGGCAGCTGTTTCATCAAGGCAAGCTCAAGTACACCCGACCGTACTTGATCGAGATCATGTATTCCAGTGATGTACAGATATCGAATCTGACCCTGCTAAATTCTCCAGCTTGGAATGTTCATCCTGTTTACAGCAGGTAATCAATCAGTCAATTCCATAGAAAAATATGTACAAAATTCAACACTCCCTGATTTGATTCACTGGCAAATGTGTTATACAGCAATGTTCTTATTCAAGGCATCACCATTATCGCTCCAGTTCGATCCCCGAATACAGATGGTATCAACCCAGGTTTGTATCCCAAAGCAACTCAAACTTCTGGGGAAGACAACGTTTTACACTTTGTTAAATCAATGACTTATTTCATCTGCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTAGCTGTCAAGAGTGGTTGGGATGAGTATGGCATAAGATTTGGATGGCCGACTAAGCAATTGATCGTCAGACGGCTCACGTGCATTTCCCCAACAAGTGCTGTCATCGCACTGGGGAGTGAGATGTCGGGCGGGATCGAAGACGTCCGAGCTGAAGACATTGTAGCCATCAATTCTGAATCTGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGCTATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACTGGTGATTACGGGTCACATGCTGATAAGAATTACGACCCGCATGCATTGCCTATAATCCAAGGAATCAACTACAGGGACATGGTCGTTGAAAACGCCACAATGGCAGCAAGATTGGAGGGAATTTCAGGTGACACATTTACAAATATCTGTATTTCCAATGTGACAATCAGCTTGGCAGCAAAGGCCAAGAAACAGCCATGGACTTGCACCGATGTTGAGGGAATAACGAGCCAAGTTACTCCTCCACCGTGCAGTCTGTTACCCGATCAAGGGGAAGAGAAGATTACAACATGCAAATTTCCAGAGGATAATATACCAATTGATACAGTTGAGTTGAAAAAATGCTCTTATCAATTTAGTCATGCCTGA

mRNA sequence

ATGCCAATGGGGAAACTCTACAAAAACCCCATTAAAGCTCAAGTGGTGAAAATGACGATTTTGGTGCTCGTTCTGGCGGCGGCGTTGATGGGGGAAGTTGAGGGAAGGAAGGGCAAAAAGGTAAAAAAGGCGAGGATTTTGGAGGGCAATGGGATTGAATATGGGGCCATAAGTTGCAGAGCTCACAGTGCTTCGATAACGGAGTTCGGCGGCGTTGGCGACGGCGAGACGTCGAATACGAAGGCGTTTCAGACGGCGGTGGAGCTGCTGAGCCAGTACGCCGGAGACGGCGGCGCTCAGCTGTTTGTGCCGGCGGGGAGGTGGCTCACGGGGAGTTTCAATCTGACCAGTCATTTCACTCTGTTTCTGCACCGAGATGCTGTCCTTCTTGCTTCTCAGGATCCGAGTGAATGGCCTGTGGTCGAACCGTTGCCATCCTACGGTCATGGAAGGGACACATCAGGGGGAAGATATATCAGTTTAATATTTGGAACAAACCTCACTGATGTTGTCATCACAGGAAACAACGGCACCATTGATGGTCAGGGTGATCGCTGGTGGCAGCTGTTTCATCAAGGCAAGCTCAAGTACACCCGACCGTACTTGATCGAGATCATGTATTCCAGTGATGTACAGATATCGAATCTGACCCTGCTAAATTCTCCAGCTTGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTTATTCAAGGCATCACCATTATCGCTCCAGTTCGATCCCCGAATACAGATGGTATCAACCCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTAGCTGTCAAGAGTGGTTGGGATGAGTATGGCATAAGATTTGGATGGCCGACTAAGCAATTGATCGTCAGACGGCTCACGTGCATTTCCCCAACAAGTGCTGTCATCGCACTGGGGAGTGAGATGTCGGGCGGGATCGAAGACGTCCGAGCTGAAGACATTGTAGCCATCAATTCTGAATCTGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGCTATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACTGGTGATTACGGGTCACATGCTGATAAGAATTACGACCCGCATGCATTGCCTATAATCCAAGGAATCAACTACAGGGACATGGTCGTTGAAAACGCCACAATGGCAGCAAGATTGGAGGGAATTTCAGGTGACACATTTACAAATATCTGTATTTCCAATGTGACAATCAGCTTGGCAGCAAAGGCCAAGAAACAGCCATGGACTTGCACCGATGTTGAGGGAATAACGAGCCAAGTTACTCCTCCACCGTGCAGTCTGTTACCCGATCAAGGGGAAGAGAAGATTACAACATGCAAATTTCCAGAGGATAATATACCAATTGATACAGTTGAGTTGAAAAAATGCTCTTATCAATTTAGTCATGCCTGA

Coding sequence (CDS)

ATGCCAATGGGGAAACTCTACAAAAACCCCATTAAAGCTCAAGTGGTGAAAATGACGATTTTGGTGCTCGTTCTGGCGGCGGCGTTGATGGGGGAAGTTGAGGGAAGGAAGGGCAAAAAGGTAAAAAAGGCGAGGATTTTGGAGGGCAATGGGATTGAATATGGGGCCATAAGTTGCAGAGCTCACAGTGCTTCGATAACGGAGTTCGGCGGCGTTGGCGACGGCGAGACGTCGAATACGAAGGCGTTTCAGACGGCGGTGGAGCTGCTGAGCCAGTACGCCGGAGACGGCGGCGCTCAGCTGTTTGTGCCGGCGGGGAGGTGGCTCACGGGGAGTTTCAATCTGACCAGTCATTTCACTCTGTTTCTGCACCGAGATGCTGTCCTTCTTGCTTCTCAGGATCCGAGTGAATGGCCTGTGGTCGAACCGTTGCCATCCTACGGTCATGGAAGGGACACATCAGGGGGAAGATATATCAGTTTAATATTTGGAACAAACCTCACTGATGTTGTCATCACAGGAAACAACGGCACCATTGATGGTCAGGGTGATCGCTGGTGGCAGCTGTTTCATCAAGGCAAGCTCAAGTACACCCGACCGTACTTGATCGAGATCATGTATTCCAGTGATGTACAGATATCGAATCTGACCCTGCTAAATTCTCCAGCTTGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTTATTCAAGGCATCACCATTATCGCTCCAGTTCGATCCCCGAATACAGATGGTATCAACCCAGATTCCTGCACAAACGTTAGAATTGAGGATTGTTACATAGTATCTGGAGATGATTGTGTAGCTGTCAAGAGTGGTTGGGATGAGTATGGCATAAGATTTGGATGGCCGACTAAGCAATTGATCGTCAGACGGCTCACGTGCATTTCCCCAACAAGTGCTGTCATCGCACTGGGGAGTGAGATGTCGGGCGGGATCGAAGACGTCCGAGCTGAAGACATTGTAGCCATCAATTCTGAATCTGGGGTCAGGATCAAAACTGGCATAGGAAGGGGAGGCTATGTAAAAGACATATACGTAAGGAGGATGACAATGCATACCATGAAATGGGCATTTTGGATGACTGGTGATTACGGGTCACATGCTGATAAGAATTACGACCCGCATGCATTGCCTATAATCCAAGGAATCAACTACAGGGACATGGTCGTTGAAAACGCCACAATGGCAGCAAGATTGGAGGGAATTTCAGGTGACACATTTACAAATATCTGTATTTCCAATGTGACAATCAGCTTGGCAGCAAAGGCCAAGAAACAGCCATGGACTTGCACCGATGTTGAGGGAATAACGAGCCAAGTTACTCCTCCACCGTGCAGTCTGTTACCCGATCAAGGGGAAGAGAAGATTACAACATGCAAATTTCCAGAGGATAATATACCAATTGATACAGTTGAGTTGAAAAAATGCTCTTATCAATTTAGTCATGCCTGA

Protein sequence

MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDTVELKKCSYQFSHA
Homology
BLAST of Lag0020729.1 vs. NCBI nr
Match: TYK17614.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 943.3 bits (2437), Expect = 8.1e-271
Identity = 455/493 (92.29%), Postives = 476/493 (96.55%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGR+G  KK KKA+ILEGNGIEYGAI+
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAIN 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE L QYA DGGAQL+VPAGRWLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGI+DVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTDVEGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID 
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDA 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. NCBI nr
Match: XP_008438007.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0048953.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 941.4 bits (2432), Expect = 3.1e-270
Identity = 454/493 (92.09%), Postives = 475/493 (96.35%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGR+G  KK KKA+ILEGNGIEYGAI+
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAIN 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE L QYA DGGAQL+VPAGRWLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYS D+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSRDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGI+DVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTDVEGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID 
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDA 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. NCBI nr
Match: XP_004133855.1 (probable polygalacturonase [Cucumis sativus] >KGN56496.1 hypothetical protein Csa_011241 [Cucumis sativus])

HSP 1 Score: 939.5 bits (2427), Expect = 1.2e-269
Identity = 453/493 (91.89%), Postives = 476/493 (96.55%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGRKG  KK KKA+ILEGNGIEYGAIS
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIVAAALMDEVEGRKGKVKKGKKAKILEGNGIEYGAIS 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE LSQY  DGGAQL+VPAG+WLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVEQLSQYTDDGGAQLYVPAGKWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI FG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGISFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAI+SESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINY+DMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYKDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTD+EGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID+
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDIEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDS 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. NCBI nr
Match: XP_022974569.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 935.3 bits (2416), Expect = 2.2e-268
Identity = 452/491 (92.06%), Postives = 469/491 (95.52%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCR 60
           MPM +L KNPIKA  VKMT LVLVLAAALMGEVEGR      KARILE NGIEYGAISCR
Sbjct: 1   MPMEELCKNPIKADAVKMTFLVLVLAAALMGEVEGR------KARILEDNGIEYGAISCR 60

Query: 61  AHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFT 120
           AH+ASITEFGGVGDGETSNTKAFQ AVE LSQYAGDGGAQL+VPAGRWLTGSFNLTSHFT
Sbjct: 61  AHTASITEFGGVGDGETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFT 120

Query: 121 LFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID 180
           LFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID
Sbjct: 121 LFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID 180

Query: 181 GQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGIT 240
           GQGDRWWQLFHQ KLKYTRPYLIEIMYSS++QISNLTLLNSPAWNVHPVYSSNVLIQGIT
Sbjct: 181 GQGDRWWQLFHQNKLKYTRPYLIEIMYSSNIQISNLTLLNSPAWNVHPVYSSNVLIQGIT 240

Query: 241 IIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRL 300
           IIAPVRSPNTDGINPDSCTNVRIEDCYI+SGDDCVAVKSGWDEYGIRFGWPTKQL++RRL
Sbjct: 241 IIAPVRSPNTDGINPDSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRL 300

Query: 301 TCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMH 360
           TC+SPTSAVIALGSEMSGGIEDVRAEDIVA++SESGVRIKTGIGRGGYVKDIYVRRMTMH
Sbjct: 301 TCVSPTSAVIALGSEMSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMH 360

Query: 361 TMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICI 420
           TMKWAFWMTGDYGSHAD+NYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT ICI
Sbjct: 361 TMKWAFWMTGDYGSHADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICI 420

Query: 421 SNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDTVE 480
           SNVTISLAAKAKKQPWTCTDVEG TS VTPPPCSLLPDQG EK TTC+FPE+NIPID VE
Sbjct: 421 SNVTISLAAKAKKQPWTCTDVEGTTSNVTPPPCSLLPDQGAEKFTTCRFPEENIPIDAVE 480

Query: 481 LKKCSYQFSHA 492
           LKKCSYQ SHA
Sbjct: 481 LKKCSYQLSHA 485

BLAST of Lag0020729.1 vs. NCBI nr
Match: XP_022979338.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 931.8 bits (2407), Expect = 2.4e-267
Identity = 447/489 (91.41%), Postives = 472/489 (96.52%), Query Frame = 0

Query: 3   MGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCRAH 62
           M +LYK PIKAQVVKM ILVL+LAAA+MGEVEGRKG+K KK RI+EG  IEYGAISCRAH
Sbjct: 1   MEELYKKPIKAQVVKMAILVLILAAAMMGEVEGRKGRKHKKGRIVEGKMIEYGAISCRAH 60

Query: 63  SASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFTLF 122
           SASIT+FGGVGDGETSNTKAFQ AVE LSQYAGDGGAQL+VPAGRWLTGSFNLTSHFTLF
Sbjct: 61  SASITDFGGVGDGETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFTLF 120

Query: 123 LHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQ 182
           LH+DAVLLASQD +EW VVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQ
Sbjct: 121 LHQDAVLLASQDLNEWSVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQ 180

Query: 183 GDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGITII 242
           GDRWWQLFHQGKLKYTRPYLIEIMYSS++QISNLTLLNSP+WNVHPVYSSN++IQGITII
Sbjct: 181 GDRWWQLFHQGKLKYTRPYLIEIMYSSNIQISNLTLLNSPSWNVHPVYSSNLVIQGITII 240

Query: 243 APVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRLTC 302
           APVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQL++RRLTC
Sbjct: 241 APVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLVIRRLTC 300

Query: 303 ISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTM 362
           ISPTSAVIALGSEMSGGI+DVRAEDI AINSESGVRIKTGIGRGGYVKDIYVRRMTMHTM
Sbjct: 301 ISPTSAVIALGSEMSGGIQDVRAEDITAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTM 360

Query: 363 KWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICISN 422
           KWAFWMTG+YGSHADK YDPHALP+IQGINYRD+VVENATMAARLEGISGD+FT+ICISN
Sbjct: 361 KWAFWMTGNYGSHADKKYDPHALPVIQGINYRDIVVENATMAARLEGISGDSFTDICISN 420

Query: 423 VTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDTVELK 482
           VTIS+A KAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPED IPI+ VEL+
Sbjct: 421 VTISMAPKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDTIPIEAVELR 480

Query: 483 KCSYQFSHA 492
           KCSYQ SHA
Sbjct: 481 KCSYQISHA 489

BLAST of Lag0020729.1 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.0e-220
Identity = 368/477 (77.15%), Postives = 412/477 (86.37%), Query Frame = 0

Query: 14  QVVKMTILVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCRAHSASITEFGGVG 73
           QVV + + V++L +   GE         +K RILE   +EY AISCRAHSAS+ +FGGVG
Sbjct: 23  QVVSVLVAVVLLLSVSRGE--------CRKGRILE--ALEYSAISCRAHSASLVDFGGVG 82

Query: 74  DGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFTLFLHRDAVLLASQ 133
           DG+T NTKAFQ AV  LS+Y  +GGAQL+VPAG+WLTGSF+LTSHFTLFLHRDAVLLASQ
Sbjct: 83  DGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQ 142

Query: 134 DPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQG 193
           D S+WPV++PLPSYG GRD + GRY SLIFGTNLTDV+ITG+NGTIDGQG  WWQ FH G
Sbjct: 143 DISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGG 202

Query: 194 KLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGI 253
           KLKYTRPYLIE+MYS+D+QISNLTLLNSP+WNVHPVYS N+LIQGITI+APVRSPNTDGI
Sbjct: 203 KLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGI 262

Query: 254 NPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRLTCISPTSAVIALG 313
           NPDSCTN RIEDCYIVSGDDCVAVKSGWDEYGI +G PTKQL++RRLTCISP SAVIALG
Sbjct: 263 NPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALG 322

Query: 314 SEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYG 373
           SEMSGGI+DVRAEDIVAINSESG+RIKTGIGRGGYVKDIYVR MTM TMKWAFWMTG+YG
Sbjct: 323 SEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYG 382

Query: 374 SHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICISNVTISLAAKAKK 433
           SHAD +YDP A P+IQGINYRDMV EN +MAARLEGI  D FT ICISNVTI LAAKAKK
Sbjct: 383 SHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKK 442

Query: 434 QPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDTVELKKCSYQFSH 491
            PWTCTDVEGI+S VTP PCS LPDQG EK + C FP +++PIDTVEL+KCSY  ++
Sbjct: 443 VPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKCSYGINY 489

BLAST of Lag0020729.1 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.8e-26
Identity = 118/411 (28.71%), Postives = 180/411 (43.80%), Query Frame = 0

Query: 65  SITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGS---FNLTSHFTL 124
           ++ ++G VGDG+  +T+AF T      + A    A L VPA +    +   F       L
Sbjct: 60  NVEQYGAVGDGKHDSTEAFATTWNAACKKA---SAVLLVPANKKFFVNNLVFRGPCQPHL 119

Query: 125 FLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTIDG 184
               D  ++A  DP+ W                    I L F   LTD  + G  G IDG
Sbjct: 120 SFKVDGTIVAQPDPARWK----------------NSKIWLQF-AQLTDFNLMG-TGVIDG 179

Query: 185 QGDRWWQLFHQGKLKY----------TRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYS 244
           QG +WW     G+ K            RP  I+I YS  V +  LTL+NSP +++     
Sbjct: 180 QGQQWW----AGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGEC 239

Query: 245 SNVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWP 304
             V IQG+ I AP  SPNTDGI+  +     IE C I +GDDC+A+ +G           
Sbjct: 240 EGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG----------- 299

Query: 305 TKQLIVRRLTCISPTSAVI-ALGSEMS-GGIEDVRAEDIVAINSESGVRIKTGIGRGGYV 364
           +  + ++ L C       I +LG + S   +  V       I++++G+RIKT  G  G  
Sbjct: 300 SSNITIKDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLA 359

Query: 365 KDIYVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEG 424
             I    + M   +    +   Y + A    +  +   IQG+ Y+++   +AT AA ++ 
Sbjct: 360 SYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSAT-AAAIQL 419

Query: 425 ISGDTF--TNICISNVTISLAAKAKKQPWTCTD--VEGITSQVTPPPCSLL 457
           +  D+   T I +SNV++ L +    +P +C D    G  S    P C  L
Sbjct: 420 MCSDSVPCTGIQLSNVSLKLTS---GKPASCVDKNARGFYSGRLIPTCKNL 430

BLAST of Lag0020729.1 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 8.8e-26
Identity = 98/314 (31.21%), Postives = 154/314 (49.04%), Query Frame = 0

Query: 61  AHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRW-------LTGSF 120
           +++  +T+FG VGDG T +++AF  A E +    GDG  Q  VPAG           GS 
Sbjct: 21  SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG--QFVVPAGMTFMLQPLKFQGSC 80

Query: 121 NLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVIT 180
             T  F   L +   L+A             PS G+ +    G     I  T++  +VI 
Sbjct: 81  KSTPVFVQMLGK---LVA-------------PSKGNWK----GDKDQWILFTDIEGLVIE 140

Query: 181 GNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSN 240
           G +G I+GQG  WW+  H+G    +RP  ++    +++++S LT L+SP  ++H    + 
Sbjct: 141 G-DGEINGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNY 200

Query: 241 VLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTK 300
           V I  + I AP  SPNTDGI+  + +NV I+DC I +GDDC+A+ SG           T 
Sbjct: 201 VTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TS 260

Query: 301 QLIVRRLTCISPTSAVIALGSEMSGG----IEDVRAEDIVAINSESGVRIKTGIGRGGYV 360
            + +  + C  P    I++GS    G    +E+V  ++     + +G RIKT  G  GY 
Sbjct: 261 NIHISGIDC-GPGHG-ISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYA 292

Query: 361 KDIYVRRMTMHTMK 364
           + I    +T+  ++
Sbjct: 321 RMITFNGITLDNVE 292

BLAST of Lag0020729.1 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.8e-24
Identity = 72/211 (34.12%), Postives = 104/211 (49.29%), Query Frame = 0

Query: 212 QISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSG 271
           Q+   T+ N+ +W +HP    ++     TIIAP  SPNTDG NP+SC NV I       G
Sbjct: 34  QLLGFTIRNAASWTIHPQGCEDLTAAASTIIAPHDSPNTDGFNPESCRNVMISGVRFSVG 93

Query: 272 DDCVAVKS------GWDEYGIRFGWPTKQLIVRRLTCISPTSAVIALGSEMSGGIEDVRA 331
           DDC+AVK+      G D++       T+ + VR    + P    + +GSEMSGG+ DV  
Sbjct: 94  DDCIAVKAGKRGPDGEDDHLAE----TRGITVRH-CLMQPGHGGLVIGSEMSGGVHDVTV 153

Query: 332 EDIVAINSESGVRIKTGI-GRGGYVKDIYVRRMTMHTMKWAFWMTGDYGSHADKNYD--- 391
           ED   I ++ G+R+KTG    GG V +I +RR+ +  ++ A      Y   AD + D   
Sbjct: 154 EDCDMIGTDRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHCDADGHDDWVQ 213

Query: 392 -------PHALPIIQGINYRDMVVENATMAA 406
                      P + GI   D+ + N   AA
Sbjct: 214 SRNPAPVNDGTPFVDGITVEDVEIRNLAHAA 239

BLAST of Lag0020729.1 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.6e-22
Identity = 110/385 (28.57%), Postives = 172/385 (44.68%), Query Frame = 0

Query: 65  SITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLT------SH 124
           ++  +G VGDG+  +T AF+   +  +       A   VPA +    + NL        H
Sbjct: 61  NVEHYGAVGDGKHDSTDAFE---KTWNAACNKLSAVFLVPANKKFVVN-NLVFYGPCQPH 120

Query: 125 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 184
           F+  +  D  + A  DP++W                    I + F   LTD  + G  G 
Sbjct: 121 FSFKV--DGTIAAYPDPAKWK----------------NSKIWMHF-ARLTDFNLMG-TGV 180

Query: 185 IDGQGDRWWQLFHQGKL--------KYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVY 244
           IDGQG+RWW    Q K            RP  I+I +S  V +  LTL NSP +++    
Sbjct: 181 IDGQGNRWWS--DQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGE 240

Query: 245 SSNVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGW 304
              V IQGI I AP  SPNTDGI+  +     IE C I +GDDCVAV +G          
Sbjct: 241 CDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG---------- 300

Query: 305 PTKQLIVRRLTCISPTSAVIALGSEMSGG----IEDVRAEDIVAINSESGVRIKTGIGRG 364
            +  + ++ LTC  P    +++GS   G     +  V  +    I++++G+RIKT  G  
Sbjct: 301 -SSNITIKDLTC-GPGHG-MSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGS 360

Query: 365 GYVKDIYVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAAR 424
           G    I    + M   +    +   Y + A    +  +   IQ + ++++   +AT AA 
Sbjct: 361 GLASHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAA- 405

Query: 425 LEGISGDTF--TNICISNVTISLAA 430
           ++ +  D+   +NI +SNV + L +
Sbjct: 421 IQLMCSDSVPCSNIKLSNVFLKLTS 405

BLAST of Lag0020729.1 vs. ExPASy TrEMBL
Match: A0A5D3D2R4 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004840 PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 3.9e-271
Identity = 455/493 (92.29%), Postives = 476/493 (96.55%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGR+G  KK KKA+ILEGNGIEYGAI+
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAIN 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE L QYA DGGAQL+VPAGRWLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGI+DVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTDVEGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID 
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDA 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. ExPASy TrEMBL
Match: A0A5A7TZA9 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001740 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 454/493 (92.09%), Postives = 475/493 (96.35%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGR+G  KK KKA+ILEGNGIEYGAI+
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAIN 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE L QYA DGGAQL+VPAGRWLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYS D+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSRDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGI+DVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTDVEGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID 
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDA 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. ExPASy TrEMBL
Match: A0A1S3AUZ9 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103483253 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 454/493 (92.09%), Postives = 475/493 (96.35%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGR+G  KK KKA+ILEGNGIEYGAI+
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIMAAALMDEVEGRRGKVKKGKKAKILEGNGIEYGAIN 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE L QYA DGGAQL+VPAGRWLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVERLGQYANDGGAQLYVPAGRWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYS D+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSRDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGI+DVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTDVEGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID 
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDVEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDA 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. ExPASy TrEMBL
Match: A0A0A0L8V4 (Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121720 PE=3 SV=1)

HSP 1 Score: 939.5 bits (2427), Expect = 5.7e-270
Identity = 453/493 (91.89%), Postives = 476/493 (96.55%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKG--KKVKKARILEGNGIEYGAIS 60
           MPM KLYKNPIKAQV+KM IL +++AAALM EVEGRKG  KK KKA+ILEGNGIEYGAIS
Sbjct: 1   MPMEKLYKNPIKAQVMKMVILGVIVAAALMDEVEGRKGKVKKGKKAKILEGNGIEYGAIS 60

Query: 61  CRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSH 120
           CRAH+ASITEFGGVGDG TSNTKAFQ AVE LSQY  DGGAQL+VPAG+WLTGSFNLTSH
Sbjct: 61  CRAHTASITEFGGVGDGVTSNTKAFQAAVEQLSQYTDDGGAQLYVPAGKWLTGSFNLTSH 120

Query: 121 FTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180
           FTLFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT
Sbjct: 121 FTLFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGT 180

Query: 181 IDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQG 240
           IDGQGDRWWQLFHQGKLKYTRPYLIE+MYSSD+QIS+LTLLNSPAWNVHPVYSSN+LIQG
Sbjct: 181 IDGQGDRWWQLFHQGKLKYTRPYLIELMYSSDIQISSLTLLNSPAWNVHPVYSSNILIQG 240

Query: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVR 300
           ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI FG PTKQL+VR
Sbjct: 241 ITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGISFGLPTKQLVVR 300

Query: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMT 360
           RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAI+SESGVRIKTGIGRGGYVKDIYVRRMT
Sbjct: 301 RLTCISPTSAVIALGSEMSGGIEDVRAEDIVAIDSESGVRIKTGIGRGGYVKDIYVRRMT 360

Query: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNI 420
           MHTMKWAFWMTGDYGSHADKNYDPHALP+IQGINY+DMVVENATMAARLEGISGDTFT+I
Sbjct: 361 MHTMKWAFWMTGDYGSHADKNYDPHALPVIQGINYKDMVVENATMAARLEGISGDTFTDI 420

Query: 421 CISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDT 480
           CISNVTI LA KAKKQPWTCTD+EGITSQVTPPPC LLPDQGEEKITTCKFPEDN+PID+
Sbjct: 421 CISNVTIGLAPKAKKQPWTCTDIEGITSQVTPPPCDLLPDQGEEKITTCKFPEDNLPIDS 480

Query: 481 VELKKCSYQFSHA 492
           V+LKKCSYQFSHA
Sbjct: 481 VQLKKCSYQFSHA 493

BLAST of Lag0020729.1 vs. ExPASy TrEMBL
Match: A0A6J1II01 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111473265 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.1e-268
Identity = 452/491 (92.06%), Postives = 469/491 (95.52%), Query Frame = 0

Query: 1   MPMGKLYKNPIKAQVVKMTILVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCR 60
           MPM +L KNPIKA  VKMT LVLVLAAALMGEVEGR      KARILE NGIEYGAISCR
Sbjct: 1   MPMEELCKNPIKADAVKMTFLVLVLAAALMGEVEGR------KARILEDNGIEYGAISCR 60

Query: 61  AHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFT 120
           AH+ASITEFGGVGDGETSNTKAFQ AVE LSQYAGDGGAQL+VPAGRWLTGSFNLTSHFT
Sbjct: 61  AHTASITEFGGVGDGETSNTKAFQAAVERLSQYAGDGGAQLYVPAGRWLTGSFNLTSHFT 120

Query: 121 LFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID 180
           LFLH+DAVLLASQDP+EWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID
Sbjct: 121 LFLHQDAVLLASQDPNEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVITGNNGTID 180

Query: 181 GQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGIT 240
           GQGDRWWQLFHQ KLKYTRPYLIEIMYSS++QISNLTLLNSPAWNVHPVYSSNVLIQGIT
Sbjct: 181 GQGDRWWQLFHQNKLKYTRPYLIEIMYSSNIQISNLTLLNSPAWNVHPVYSSNVLIQGIT 240

Query: 241 IIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRL 300
           IIAPVRSPNTDGINPDSCTNVRIEDCYI+SGDDCVAVKSGWDEYGIRFGWPTKQL++RRL
Sbjct: 241 IIAPVRSPNTDGINPDSCTNVRIEDCYIISGDDCVAVKSGWDEYGIRFGWPTKQLVIRRL 300

Query: 301 TCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMH 360
           TC+SPTSAVIALGSEMSGGIEDVRAEDIVA++SESGVRIKTGIGRGGYVKDIYVRRMTMH
Sbjct: 301 TCVSPTSAVIALGSEMSGGIEDVRAEDIVAVDSESGVRIKTGIGRGGYVKDIYVRRMTMH 360

Query: 361 TMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICI 420
           TMKWAFWMTGDYGSHAD+NYDPHALP+IQGINYRDMVVENATMAARLEGISGDTFT ICI
Sbjct: 361 TMKWAFWMTGDYGSHADENYDPHALPVIQGINYRDMVVENATMAARLEGISGDTFTGICI 420

Query: 421 SNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCKFPEDNIPIDTVE 480
           SNVTISLAAKAKKQPWTCTDVEG TS VTPPPCSLLPDQG EK TTC+FPE+NIPID VE
Sbjct: 421 SNVTISLAAKAKKQPWTCTDVEGTTSNVTPPPCSLLPDQGAEKFTTCRFPEENIPIDAVE 480

Query: 481 LKKCSYQFSHA 492
           LKKCSYQ SHA
Sbjct: 481 LKKCSYQLSHA 485

BLAST of Lag0020729.1 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-199
Identity = 324/445 (72.81%), Postives = 376/445 (84.49%), Query Frame = 0

Query: 53  EYGAISCRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGS 112
           EY AI CR+HSASITE+GGVGDG+T NTKAFQ+AV+ LSQY+ +GGAQLFVPAG+WLTGS
Sbjct: 34  EYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGS 93

Query: 113 FNLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVI 172
           FNLTSHFTLFLH+DA+LLA+QD +E+P+++ LPSYG GRD +GGR+ SLIFGTNL+DV+I
Sbjct: 94  FNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVII 153

Query: 173 TGNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSS 232
           TGNNGTIDGQG  WWQ FH GKLKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSS
Sbjct: 154 TGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSS 213

Query: 233 NVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPT 292
           N++++G+TIIAPV+SPNTDGINPDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI FG PT
Sbjct: 214 NIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 273

Query: 293 KQLIVRRLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDI 352
           K L++RRLTCISP SA IALGSEMSGGIEDVRAEDI A  +ESGVRIKT +GRG +VK+I
Sbjct: 274 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 333

Query: 353 YVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISG 412
           YV+ M +HTMKW FWMTG+Y +HAD +YDPHALP I GINYRD+V EN +MA RLEGISG
Sbjct: 334 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 393

Query: 413 DTFTNICISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITT------ 472
           D FT ICISN TIS+AAK KK  W C+DVEG+TS V P PC LL   G+E  TT      
Sbjct: 394 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLL--DGQESETTKKKMID 453

Query: 473 --CKFPEDNIPIDTVELKKCSYQFS 490
             C FP D + ID VELK CSYQ S
Sbjct: 454 GGCDFPTDVLEIDNVELKTCSYQMS 476

BLAST of Lag0020729.1 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-199
Identity = 324/445 (72.81%), Postives = 376/445 (84.49%), Query Frame = 0

Query: 53  EYGAISCRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGS 112
           EY AI CR+HSASITE+GGVGDG+T NTKAFQ+AV+ LSQY+ +GGAQLFVPAG+WLTGS
Sbjct: 34  EYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGS 93

Query: 113 FNLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVI 172
           FNLTSHFTLFLH+DA+LLA+QD +E+P+++ LPSYG GRD +GGR+ SLIFGTNL+DV+I
Sbjct: 94  FNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVII 153

Query: 173 TGNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSS 232
           TGNNGTIDGQG  WWQ FH GKLKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSS
Sbjct: 154 TGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSS 213

Query: 233 NVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPT 292
           N++++G+TIIAPV+SPNTDGINPDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI FG PT
Sbjct: 214 NIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 273

Query: 293 KQLIVRRLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDI 352
           K L++RRLTCISP SA IALGSEMSGGIEDVRAEDI A  +ESGVRIKT +GRG +VK+I
Sbjct: 274 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 333

Query: 353 YVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISG 412
           YV+ M +HTMKW FWMTG+Y +HAD +YDPHALP I GINYRD+V EN +MA RLEGISG
Sbjct: 334 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 393

Query: 413 DTFTNICISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITT------ 472
           D FT ICISN TIS+AAK KK  W C+DVEG+TS V P PC LL   G+E  TT      
Sbjct: 394 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLL--DGQESETTKKKMID 453

Query: 473 --CKFPEDNIPIDTVELKKCSYQFS 490
             C FP D + ID VELK CSYQ S
Sbjct: 454 GGCDFPTDVLEIDNVELKTCSYQMS 476

BLAST of Lag0020729.1 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-199
Identity = 324/445 (72.81%), Postives = 376/445 (84.49%), Query Frame = 0

Query: 53  EYGAISCRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRWLTGS 112
           EY AI CR+HSASITE+GGVGDG+T NTKAFQ+AV+ LSQY+ +GGAQLFVPAG+WLTGS
Sbjct: 34  EYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGS 93

Query: 113 FNLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLTDVVI 172
           FNLTSHFTLFLH+DA+LLA+QD +E+P+++ LPSYG GRD +GGR+ SLIFGTNL+DV+I
Sbjct: 94  FNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVII 153

Query: 173 TGNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSS 232
           TGNNGTIDGQG  WWQ FH GKLKYTRPYLIE+M+S  +QISNLT L+SP+WN+HPVYSS
Sbjct: 154 TGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSS 213

Query: 233 NVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPT 292
           N++++G+TIIAPV+SPNTDGINPDSCTN RIEDCYI+SGDDC+AVKSGWDEYGI FG PT
Sbjct: 214 NIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 273

Query: 293 KQLIVRRLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGYVKDI 352
           K L++RRLTCISP SA IALGSEMSGGIEDVRAEDI A  +ESGVRIKT +GRG +VK+I
Sbjct: 274 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 333

Query: 353 YVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLEGISG 412
           YV+ M +HTMKW FWMTG+Y +HAD +YDPHALP I GINYRD+V EN +MA RLEGISG
Sbjct: 334 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 393

Query: 413 DTFTNICISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITT------ 472
           D FT ICISN TIS+AAK KK  W C+DVEG+TS V P PC LL   G+E  TT      
Sbjct: 394 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLL--DGQESETTKKKMID 453

Query: 473 --CKFPEDNIPIDTVELKKCSYQFS 490
             C FP D + ID VELK CSYQ S
Sbjct: 454 GGCDFPTDVLEIDNVELKTCSYQMS 476

BLAST of Lag0020729.1 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 686.0 bits (1769), Expect = 2.2e-197
Identity = 323/436 (74.08%), Postives = 369/436 (84.63%), Query Frame = 0

Query: 49  GNGIEYGAISCRAHSASITEFGGVGDGETSNTKAFQTAVELLSQYAGDGGAQLFVPAGRW 108
           G   EY AISCRA+SAS+ EFG VGDG TSNT AF+ AV  LS++A  GG+ LFVPAGRW
Sbjct: 53  GEEFEYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRW 112

Query: 109 LTGSFNLTSHFTLFLHRDAVLLASQDPSEWPVVEPLPSYGHGRDTSGGRYISLIFGTNLT 168
           LTG+FNLTSHFTLFLHRDAV+LASQ+ S++ V+EPLPSYG GRDT GGR+ISL+FG+NLT
Sbjct: 113 LTGNFNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLT 172

Query: 169 DVVITGNNGTIDGQGDRWWQLFHQGKLKYTRPYLIEIMYSSDVQISNLTLLNSPAWNVHP 228
           DVVITG NGTIDGQG+ WW  F +G+LKYTRPYLIEIM+S  +QISNLT LNSP+W++HP
Sbjct: 173 DVVITGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHP 232

Query: 229 VYSSNVLIQGITIIAPVRSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIRF 288
           VYSSN+ IQG+TI+APV  PNTDGINPDSCTN RIEDCYIVSGDDC+AVKSGWD+YGI +
Sbjct: 233 VYSSNIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINY 292

Query: 289 GWPTKQLIVRRLTCISPTSAVIALGSEMSGGIEDVRAEDIVAINSESGVRIKTGIGRGGY 348
           G PTKQL++RRLTCISP SAVIALGSEMSGGIEDVRAEDIVAINSESG+RIKT IGRGGY
Sbjct: 293 GMPTKQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTAIGRGGY 352

Query: 349 VKDIYVRRMTMHTMKWAFWMTGDYGSHADKNYDPHALPIIQGINYRDMVVENATMAARLE 408
           VKD+YVR MTM TMK+ FWMTG YGSH D +YDP ALP+IQ INY+DMV EN TM A+L 
Sbjct: 353 VKDVYVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAENVTMPAQLA 412

Query: 409 GISGDTFTNICISNVTISLAAKAKKQPWTCTDVEGITSQVTPPPCSLLPDQGEEKITTCK 468
           GISGD FT ICISNVTI+L+ K KK  W CTDV G TS VTP PC LLP++    +  C 
Sbjct: 413 GISGDQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLLPEKQPGTVVPCN 472

Query: 469 FPEDNIPIDTVELKKC 485
           FPED IPID V+L++C
Sbjct: 473 FPEDPIPIDEVKLQRC 488

BLAST of Lag0020729.1 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 634.4 bits (1635), Expect = 7.5e-182
Identity = 301/466 (64.59%), Postives = 368/466 (78.97%), Query Frame = 0

Query: 21  LVLVLAAALMGEVEGRKGKKVKKARILEGNGIEYGAISCRAHSASITEFGGVGDGETSNT 80
           L++ LA  L   +E R  +    ++      IE+ A++CR HSA +T+FG VGDG+TSNT
Sbjct: 8   LLVFLAIFLFPAIESRSHRNSVTSK------IEFSALNCRKHSAILTDFGAVGDGKTSNT 67

Query: 81  KAFQTAVELLSQYAGDGGAQLFVPAGRWLTGSFNLTSHFTLFLHRDAVLLASQDPSEWPV 140
           KAF+ A+  LSQ A DGGAQL VP G+WLTGSFNLTSHFTLF+ R A +LASQD SEWPV
Sbjct: 68  KAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQRGATILASQDESEWPV 127

Query: 141 VEPLPSYGHGRDTSG-GRYISLIFGTNLTDVVITGNNGTIDGQGDRWWQLFHQGKLKYTR 200
           + PLPSYG GRD +G GR+ SLI GTNLTDVVITGNNGTI+GQG  WW  F + + K TR
Sbjct: 128 IAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQYWWDKFKKKQFKITR 187

Query: 201 PYLIEIMYSSDVQISNLTLLNSPAWNVHPVYSSNVLIQGITIIAPVRSPNTDGINPDSCT 260
           PYLIEI++S ++QISN+TL++SP+WN+HPVY ++V+++ +T++APV  PNTDGINPDSCT
Sbjct: 188 PYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAPVTVPNTDGINPDSCT 247

Query: 261 NVRIEDCYIVSGDDCVAVKSGWDEYGIRFGWPTKQLIVRRLTCISPTSAVIALGSEMSGG 320
           N  IEDCYIVSGDDC+AVKSGWD+YGI+FG PT+QL +RRLTCISP SA +ALGSEMSGG
Sbjct: 248 NTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMSGG 307

Query: 321 IEDVRAEDIVAINSESGVRIKTGIGRGGYVKDIYVRRMTMHTMKWAFWMTGDYGSHADKN 380
           I+DVR ED+   N+ES +RIKT +GRG YVKDIY RR+TM TMK+ FWM+G+YGSH D+ 
Sbjct: 308 IKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKYVFWMSGNYGSHPDEG 367

Query: 381 YDPHALPIIQGINYRDMVVENATMAARLEGISGDTFTNICISNVTISLAAKAKKQPWTCT 440
           +DP ALP I  INYRDM  EN TM+A L+GI  D FT ICISNVTI+LAAKAKK  W CT
Sbjct: 368 FDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVTIALAAKAKKMQWNCT 427

Query: 441 DVEGITSQVTPPPCSLLPD-QGEEKITTCKFPEDNIPIDTVELKKC 485
           DV G+TS+VTP PCSLLP+ + + K   C FP D IPI++V LKKC
Sbjct: 428 DVAGVTSRVTPEPCSLLPEKKAQAKNVDCAFPSDLIPIESVVLKKC 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK17614.18.1e-27192.29putative polygalacturonase [Cucumis melo var. makuwa][more]
XP_008438007.13.1e-27092.09PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0048953.1 putative poly... [more]
XP_004133855.11.2e-26991.89probable polygalacturonase [Cucumis sativus] >KGN56496.1 hypothetical protein Cs... [more]
XP_022974569.12.2e-26892.06probable polygalacturonase [Cucurbita maxima][more]
XP_022979338.12.4e-26791.41probable polygalacturonase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A7PZL31.0e-22077.15Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q7M1E71.8e-2628.71Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9LW078.8e-2631.21Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
P276444.8e-2434.12Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1[more]
Q9FY192.6e-2228.57Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D2R43.9e-27192.29Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A5A7TZA91.5e-27092.09Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3AUZ91.5e-27092.09probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103483253 PE=3 SV=1[more]
A0A0A0L8V45.7e-27091.89Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G12... [more]
A0A6J1II011.1e-26892.06probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111473265 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G61490.12.3e-19972.81Pectin lyase-like superfamily protein [more]
AT3G61490.22.3e-19972.81Pectin lyase-like superfamily protein [more]
AT3G61490.32.3e-19972.81Pectin lyase-like superfamily protein [more]
AT4G23500.12.2e-19774.08Pectin lyase-like superfamily protein [more]
AT3G48950.17.5e-18264.59Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 349..370
e-value: 9300.0
score: 0.3
coord: 258..279
e-value: 5.6
score: 15.9
coord: 231..257
e-value: 350.0
score: 10.0
coord: 320..341
e-value: 5200.0
score: 2.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 53..449
e-value: 6.6E-113
score: 379.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 161..442
e-value: 5.0E-38
score: 131.0
NoneNo IPR availablePANTHERPTHR31339:SF50PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 16..488
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 16..488
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 58..458

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0020729Lag0020729gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0020729.1.exon6Lag0020729.1.exon6exon
Lag0020729.1.exon5Lag0020729.1.exon5exon
Lag0020729.1.exon4Lag0020729.1.exon4exon
Lag0020729.1.exon3Lag0020729.1.exon3exon
Lag0020729.1.exon2Lag0020729.1.exon2exon
Lag0020729.1.exon1Lag0020729.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0020729.1cds.Lag0020729.1_6CDS
cds.Lag0020729.1cds.Lag0020729.1_5CDS
cds.Lag0020729.1cds.Lag0020729.1_4CDS
cds.Lag0020729.1cds.Lag0020729.1_3CDS
cds.Lag0020729.1cds.Lag0020729.1_2CDS
cds.Lag0020729.1cds.Lag0020729.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0020729.1Lag0020729.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity