Lag0020179.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0020179.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWAT1-related protein
Locationchr5: 48609967 .. 48612802 (-)
Sequence length939
RNA-Seq ExpressionLag0020179.1
SyntenyLag0020179.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTGAATAAAGCTGAGAAATTGTTTAGGGTCTCTCGCCCGGTTCTTGCCATGTTGCTGGTCCAAATTTTTATCACTGGAATACAACTTCTTAGCAAGGTTATTCTCAACCATGGCACCTTCATTTTTGCACTCATGTCCTACCGACATCTCGTTGCTGCTCTCTGCGTTGCACCCTTTGCTGTCTTCTTCCAAAGGTCTCTTCTTTCTTTATTCTCTTCTTTTTCACCATCAAGAAATAATAATAATTATTATTATTATTATTGTTTCATTATTGTCGTGCTTTTGTTTTGGTTGCAGAAAAAACGCAAACAAGTTAAGTTGCCAGGTTTTCTTCTGGCTCTTTCTCAGTGCCTCCACCGGGTATGTGTATCATCTGTATGCCACTCCTCTCTCTTGCTTTTATTATTACTAGTAATTAAAGAGACAACTACAACGCCAAATCTCATCATTTCAATTTAAAGAAAATTTACATAATATGAAATTAATTATTATCATTCCAGCCTCATTTTCATTTAATAACAAAAATTAATTAATACATTGTCACGAATATACATACAAACACAAATAAATAGGATCAAAATATTTTTTAAATACAATATATTTAAGTGATTATATATGTATTGTGTTATAATTTTGTCTATTCAATGGTTAATTTACTCTTCCATTTTCCTTTTTCCATATATATAAAAAATAAACATTATATTAAAAAGAAAGAAAAATTAAGAACAAAAAATATAATTGCAAATGAAGAACCTTCCCCAGTTTTATTTTTCTTAATTCTCCCTCTCCTCTTTTGTTGTTGTTCACCCATAAACTAAACCCTCTTTCTTTCTCAATCTTCCTCTCTACGGTTTTTTTTTTTTTTTTTTTTAATCTTTCACCCAATAATCTTCCACCTATATTATCACGTTTCTTTCTCTCTTCGATCGAATTCTTTTGATCTTCAGCAGCTACTTGCTAATGCTAACAAATGTATATAAGGATAGAGGAAGGAATAAAACAGAGAAAATACTCGTCTCTTGGCTTGACTCAAAGAGAGTTTTGCAAAAATGGTATGAATTATAAAAATGGATTTTTGAGACATTTATGGAATATATATTGCAATTGCACGTCCTTCTTTTTCTCTTCTCTATCTTCCCATTTCTAGCGTCAATAATTGATTGAAATATATCATCAATATTCCGTTCCAAGTGTGAAATTATTTTTTATTAGAGCACACCCATGAATCTTAAGAAAAATATAAAATTGAAACAAAGTTAATAAAGAATAGAAAAATTTATAATACAAAGTTTGTTTCTTATCCAGCCTTTATATATTATAACAATAATGATAATAATAACCAAAGCCAGCCTAACTCACTCGTAATTGATACGTGGTATCAACCAGGAGTTAGATGTTCCAATCCTCCAACTCTCAGGTTGTCAAAGTCTGATTGAACTAAAAATAATAATAAATATATAGTTTATATAGATTATGCATTCATACTCGTATACAGCTCCACTTGCGTGTGTTTCATTCATTTCTCTCACTTGTCGTTTACACCACCTTCTCTGTCCTTTCCAAAAAATTTTAGGCACGCGTAAGTCTTTCTTTGTGTCTCTATTCCATCTCAAGATGGTATTCTGCAAACTTTGGAAAATCCACAGATTTTTTTCTCTATGTGCTCTATTGACAGACTTTAATTTTGGTTGACGGGGACGATATATTATTGTAATTGTAATTGCAATTGTAATTAAGGGTTTCTCTTTCTGTAAACTAAATTAACAATAATGAACGATATATGGTGGTATAGTTTAGTTGCGGTCAATCAATCAATTGTTAAAATATATACAATGGTAAAAGAAAAATGTATGTTATTAAATGATATGCAGAATAACAGCGGCGATGGGACTGTACTACTATGGTCTACGAGACACGACAGCTACGTATGCTACGAACTTCCTGAACTTGATTCCCGTGGTGACATTTGTCATCTCCTCCATCCTCAGGTATGCTCTACCGCCTGTACTATATAATAATATACGCATGGCATGCGATGACGTTGAAGTTTGAACTGCTACTAATACACTGGTATACTTATGTGTAGGATGGAAGAAGTGAGCTTCAAGAGAAGAGCAGGGAAATTAAAGATAGTGGGGGCAATTTTATGTGTTGGGGGAGCATTAATTACCAGTCTTTACAAAGGAAAAGGATTCCATATTGGTCATCATGTTGGCCTCAATATATCTAATTATGAGCCTCAGCCTCAGCCACAGCCAGCTCACTGGGGACGAGGCACCCTCCTGCTTGTTGGCAGCTGCTTCTCCTATGCTATTTGGTTTGTTGTCCAAGTATGTTTAATTATATATATGCATGTCTAGCTCCCTCTGCCACTGCCTAGTACTCTGCTCATTCAACGTTCCTGCCACGAACAGGTGAAGTTGCTCAAAGTGTTTCCATCCACGTATTTGGCAACCATGCTAACATGTGTCATAGCATGCATTCAGTCAACGCTGCTTGGCTTATGCCTCGACACCAACAAGGCCGCTTGGAAGTTGAGTTGGGATCTGCAGCTTCTCACCATTTTATGCTCGGTAAACTAAACTATTAACTCATATCAGATATACTGATGGAGAGAATTAAATGAACCATACATATATAACGACATATATGCATGTATTATTATTTGTGTCAGGGAGCACTGGGGACCGCAGCTACTTTTTGCTTAATAACATGGGCAATTTCAATCCAAGGGCCCACTTTCCCTTCCATGTTCAACCCCTTGAGTCTCATTTTTGTGGCACTCTCAGAAGCCATCATACTTGGGCAGGAGATTAGAGTGGGCAAGTAA

mRNA sequence

ATGGGTTTGAATAAAGCTGAGAAATTGTTTAGGGTCTCTCGCCCGGTTCTTGCCATGTTGCTGGTCCAAATTTTTATCACTGGAATACAACTTCTTAGCAAGGTTATTCTCAACCATGGCACCTTCATTTTTGCACTCATGTCCTACCGACATCTCGTTGCTGCTCTCTGCGTTGCACCCTTTGCTGTCTTCTTCCAAAGAAAAAACGCAAACAAGTTAAGTTGCCAGGTTTTCTTCTGGCTCTTTCTCAGTGCCTCCACCGGAATAACAGCGGCGATGGGACTGTACTACTATGGTCTACGAGACACGACAGCTACGTATGCTACGAACTTCCTGAACTTGATTCCCGTGGTGACATTTGTCATCTCCTCCATCCTCAGGATGGAAGAAGTGAGCTTCAAGAGAAGAGCAGGGAAATTAAAGATAGTGGGGGCAATTTTATGTGTTGGGGGAGCATTAATTACCAGTCTTTACAAAGGAAAAGGATTCCATATTGGTCATCATGTTGGCCTCAATATATCTAATTATGAGCCTCAGCCTCAGCCACAGCCAGCTCACTGGGGACGAGGCACCCTCCTGCTTGTTGGCAGCTGCTTCTCCTATGCTATTTGGTTTGTTGTCCAAGTGAAGTTGCTCAAAGTGTTTCCATCCACGTATTTGGCAACCATGCTAACATGTGTCATAGCATGCATTCAGTCAACGCTGCTTGGCTTATGCCTCGACACCAACAAGGCCGCTTGGAAGTTGAGTTGGGATCTGCAGCTTCTCACCATTTTATGCTCGGGAGCACTGGGGACCGCAGCTACTTTTTGCTTAATAACATGGGCAATTTCAATCCAAGGGCCCACTTTCCCTTCCATGTTCAACCCCTTGAGTCTCATTTTTGTGGCACTCTCAGAAGCCATCATACTTGGGCAGGAGATTAGAGTGGGCAAGTAA

Coding sequence (CDS)

ATGGGTTTGAATAAAGCTGAGAAATTGTTTAGGGTCTCTCGCCCGGTTCTTGCCATGTTGCTGGTCCAAATTTTTATCACTGGAATACAACTTCTTAGCAAGGTTATTCTCAACCATGGCACCTTCATTTTTGCACTCATGTCCTACCGACATCTCGTTGCTGCTCTCTGCGTTGCACCCTTTGCTGTCTTCTTCCAAAGAAAAAACGCAAACAAGTTAAGTTGCCAGGTTTTCTTCTGGCTCTTTCTCAGTGCCTCCACCGGAATAACAGCGGCGATGGGACTGTACTACTATGGTCTACGAGACACGACAGCTACGTATGCTACGAACTTCCTGAACTTGATTCCCGTGGTGACATTTGTCATCTCCTCCATCCTCAGGATGGAAGAAGTGAGCTTCAAGAGAAGAGCAGGGAAATTAAAGATAGTGGGGGCAATTTTATGTGTTGGGGGAGCATTAATTACCAGTCTTTACAAAGGAAAAGGATTCCATATTGGTCATCATGTTGGCCTCAATATATCTAATTATGAGCCTCAGCCTCAGCCACAGCCAGCTCACTGGGGACGAGGCACCCTCCTGCTTGTTGGCAGCTGCTTCTCCTATGCTATTTGGTTTGTTGTCCAAGTGAAGTTGCTCAAAGTGTTTCCATCCACGTATTTGGCAACCATGCTAACATGTGTCATAGCATGCATTCAGTCAACGCTGCTTGGCTTATGCCTCGACACCAACAAGGCCGCTTGGAAGTTGAGTTGGGATCTGCAGCTTCTCACCATTTTATGCTCGGGAGCACTGGGGACCGCAGCTACTTTTTGCTTAATAACATGGGCAATTTCAATCCAAGGGCCCACTTTCCCTTCCATGTTCAACCCCTTGAGTCTCATTTTTGTGGCACTCTCAGAAGCCATCATACTTGGGCAGGAGATTAGAGTGGGCAAGTAA

Protein sequence

MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLNISNYEPQPQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVGK
Homology
BLAST of Lag0020179.1 vs. NCBI nr
Match: XP_038877968.1 (WAT1-related protein At5g64700-like [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 5.4e-135
Identity = 257/313 (82.11%), Postives = 277/313 (88.50%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           M +NK EKL + S P+LAML VQIF TG+Q+LSKVILNHGTFIFALM+YRHLVA LCVAP
Sbjct: 1   MSVNKVEKLLKGSCPILAMLPVQIFATGMQVLSKVILNHGTFIFALMAYRHLVATLCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLSCQVFFWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIP VTF
Sbjct: 61  FAFFFERRNANKLSCQVFFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPAVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLN--ISNYEP 180
           VISSILR+E+V  KRRAG++ +VGAILCVGGALITSLYKGKGFHIGHH   N  ISN E 
Sbjct: 121 VISSILRIEKVRLKRRAGQVTVVGAILCVGGALITSLYKGKGFHIGHHDDDNCIISNMEA 180

Query: 181 QPQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGL 240
             +    HWGRGTLLLV SCFSYA WFVVQVKL+KVFPS YLATMLTCVIACIQSTLLGL
Sbjct: 181 GGR----HWGRGTLLLVASCFSYATWFVVQVKLVKVFPSNYLATMLTCVIACIQSTLLGL 240

Query: 241 CLDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVAL 300
           CLDTNKA+WKL WDLQLLTI  SGAL TAATFCL TWAISIQGPTFP MFNPL+LIFVA+
Sbjct: 241 CLDTNKASWKLGWDLQLLTIFYSGALATAATFCLTTWAISIQGPTFPPMFNPLALIFVAI 300

Query: 301 SEAIILGQEIRVG 312
           SEAIILG+EI+VG
Sbjct: 301 SEAIILGEEIKVG 309

BLAST of Lag0020179.1 vs. NCBI nr
Match: KAG6576614.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014666.1 WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 247/312 (79.17%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FAFFFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNA 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 312

BLAST of Lag0020179.1 vs. NCBI nr
Match: XP_022922848.1 (WAT1-related protein At5g64700-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 247/312 (79.17%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FAFFFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNA 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 312

BLAST of Lag0020179.1 vs. NCBI nr
Match: XP_023553116.1 (WAT1-related protein At5g64700-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 1.9e-132
Identity = 245/312 (78.53%), Postives = 276/312 (88.46%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FAFFFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHH-VGLNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHH   +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHDAHVNDNTNNNA 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFV +S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVGIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 312

BLAST of Lag0020179.1 vs. NCBI nr
Match: XP_022984779.1 (WAT1-related protein At5g64700-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 482.6 bits (1241), Expect = 2.5e-132
Identity = 247/312 (79.17%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FA-FFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNT 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 311

BLAST of Lag0020179.1 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 105/308 (34.09%), Postives = 170/308 (55.19%), Query Frame = 0

Query: 14  RPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKL 73
           +P L + ++Q+  T + L+SK + N G   F  + YR   A + +AP A FF+RK+A  L
Sbjct: 7   KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPL 66

Query: 74  SCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSF 133
           S   F  +F+ +  G+T ++ L    L  T+AT A      +P +TF ++ +  ME +  
Sbjct: 67  SFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKV 126

Query: 134 KRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLNISNYEPQPQP---QPAH---- 193
           K   G  K+VG  +C+GG +I ++YKG    +     L    Y  Q  P    P H    
Sbjct: 127 KSIQGTAKLVGITVCMGGVIILAIYKGPLLKL----PLCPHFYHGQEHPHRNNPGHVSGG 186

Query: 194 ---WGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTN 253
              W +G +L++ S   + +W V+Q ++LKV+PS    T L C+++ IQS ++ + L+ +
Sbjct: 187 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 246

Query: 254 KAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAII 312
            +AWKL W+L+L+ ++  G + T   + L +W I  +GP F SMF PLSL+F  LS AI+
Sbjct: 247 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 306

BLAST of Lag0020179.1 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-41
Identity = 96/300 (32.00%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 15  PVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLS 74
           P LAM+LVQI   G+ + SK+ +  G     L++YR + A +   P A F +RK   K++
Sbjct: 8   PFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKIT 67

Query: 75  CQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFK 134
            ++   +F  + TG T    LY+ GL++++ T A    NL+P VTF++++I R E V  K
Sbjct: 68  LRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIK 127

Query: 135 RRAGKLKIVGAILCVGGALITSLYKGKGFHIGH---HVGLNISNYEPQPQPQPAHWGRGT 194
           + +G+ K++G ++CV GA++ S Y G    IG    H     +  +       +++  G 
Sbjct: 128 KASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGHSNFFLGP 187

Query: 195 LLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLSW 254
            L++ +  S+A WF++Q K+ + F + Y +T+L C++  IQ   + L  D   + W LS 
Sbjct: 188 FLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLSS 247

Query: 255 DLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVG 312
            L+ ++ L +G + +A  FCL++WA+  +GP + S+F+PL L+ VA+    +L +++  G
Sbjct: 248 PLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYTG 307

BLAST of Lag0020179.1 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.8e-40
Identity = 96/301 (31.89%), Postives = 155/301 (51.50%), Query Frame = 0

Query: 17  LAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQ 76
           +AML +Q    G  ++S+  LN G        YR+++A L + PFA F ++K    ++  
Sbjct: 22  IAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLN 81

Query: 77  VFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRR 136
                F  A  GITA  G Y  GL +T+ T+A++  N +P +TF+++++LR+E+V   RR
Sbjct: 82  FLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRR 141

Query: 137 AGKLKIVGAILCVGGALITSLYKGKGFHI------GHHVGLNISNYEPQPQPQPAHWGRG 196
            G  KI+G  LCV GA + +LYKG   +        H +  N +   P     P +W  G
Sbjct: 142 DGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLGNAAPKNWTLG 201

Query: 197 TLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLS 256
            + L+G C S++ W V Q  +LK +P+    T  TC    IQ  ++    + +  AW   
Sbjct: 202 CIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFH 261

Query: 257 WDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRV 312
              +L TIL +G + +   F +  W I   GP F +++ P+  + VA+  +I LG+E  +
Sbjct: 262 SGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYL 321

BLAST of Lag0020179.1 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.8e-40
Identity = 97/304 (31.91%), Postives = 169/304 (55.59%), Query Frame = 0

Query: 14  RPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKL 73
           +P+LA++ +Q    G+ +++ V   HG   + L +YRH+VA + +APFA+ F+RK   K+
Sbjct: 10  KPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRPKM 69

Query: 74  SCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSF 133
           +  +F+ L             LYY GL++T+A+Y + F N +P VTF+++ I R+E V+F
Sbjct: 70  TLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETVNF 129

Query: 134 KRRAGKLKIVGAILCVGGALITSLYKG------KGFHIGHHVGLNISNYEPQPQPQPAHW 193
           ++     K+VG ++ VGGA+I +LYKG      K  H   H G + +       P   HW
Sbjct: 130 RKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-------PTGQHW 189

Query: 194 GRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAW 253
             GT+ ++GS  ++A +F++Q   LKV+P+      L C I  I + +  L +  + +AW
Sbjct: 190 VLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAW 249

Query: 254 KLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQE 312
           K+  D   L  + SG + +   + + +  I  +GP F + F+P+ +I  A   A++L ++
Sbjct: 250 KIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEK 306

BLAST of Lag0020179.1 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.0e-39
Identity = 96/301 (31.89%), Postives = 167/301 (55.48%), Query Frame = 0

Query: 13  SRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANK 72
           +RP ++M+++Q+ + G+ +LSK +LN G   + L+ YRH VA + +APFA +F +K   K
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 73  LSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVS 132
           ++  +FF + L           LYY G++ TTAT+AT   N++P +TFV++ I  +E V 
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 133 FKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLNISNYEPQPQPQPAHWGRGTL 192
            +      K+VG +  VGGA+I +L KG    +    G++  N             +G +
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIHSAI---KGAV 192

Query: 193 LLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDT-NKAAWKLSW 252
           L+   CFSYA + ++Q   L+ +P+    T   C++  I+ T + L ++  N +AW + W
Sbjct: 193 LVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGW 252

Query: 253 DLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVG 312
           D +LLT   SG + +A  + +    +  +GP F + F+PL +I VA+   II  +++ +G
Sbjct: 253 DTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLG 310

BLAST of Lag0020179.1 vs. ExPASy TrEMBL
Match: A0A6J1E7Y3 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.4e-134
Identity = 247/312 (79.17%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FAFFFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNA 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 312

BLAST of Lag0020179.1 vs. ExPASy TrEMBL
Match: A0A6J1JBI3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.2e-132
Identity = 247/312 (79.17%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MGLNKAEKLFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAP 60
           MG+N+ +KL + SRP+LAML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAP
Sbjct: 1   MGVNQMQKLLKASRPILAMLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAP 60

Query: 61  FAVFFQRKNANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120
           FA FF+R+NANKLS QV FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF
Sbjct: 61  FA-FFERRNANKLSWQVLFWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTF 120

Query: 121 VISSILRMEEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQ 180
           VISS+LR+E+VS KRRAG++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +     
Sbjct: 121 VISSMLRIEKVSLKRRAGRITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNT 180

Query: 181 PQPQPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLC 240
                 HWGRGTLLL+GSCFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLC
Sbjct: 181 SNEGGRHWGRGTLLLLGSCFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLC 240

Query: 241 LDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALS 300
           LDTN A+WKL WDLQLLTIL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+S
Sbjct: 241 LDTNNASWKLGWDLQLLTILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAIS 300

Query: 301 EAIILGQEIRVG 312
           E IILG+EI+VG
Sbjct: 301 EGIILGEEIKVG 311

BLAST of Lag0020179.1 vs. ExPASy TrEMBL
Match: A0A6J1E4N2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.4e-127
Identity = 237/294 (80.61%), Postives = 263/294 (89.46%), Query Frame = 0

Query: 19  MLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQVF 78
           ML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAPFA FF+R+NANKLS QV 
Sbjct: 1   MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFAFFFERRNANKLSWQVL 60

Query: 79  FWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRRAG 138
           FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+E+VS KRRAG
Sbjct: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 120

Query: 139 KLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQPQPQPAHWGRGTLLLVGS 198
           ++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +           HWGRGTLLL+GS
Sbjct: 121 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNASNEGGRHWGRGTLLLLGS 180

Query: 199 CFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLSWDLQLLT 258
           CFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLCLDTN A+WKL WDLQLLT
Sbjct: 181 CFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGWDLQLLT 240

Query: 259 ILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVG 312
           IL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+SE IILG+EI+VG
Sbjct: 241 ILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 294

BLAST of Lag0020179.1 vs. ExPASy TrEMBL
Match: A0A6J1CI51 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111011392 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.6e-127
Identity = 244/313 (77.96%), Postives = 264/313 (84.35%), Query Frame = 0

Query: 9   LFRVSRPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRK 68
           LF  SRPVLAMLLVQ+F TG+QLLSK+ILN GTFIFALM+YRH+VAALCVAPFA FF R 
Sbjct: 14  LFNASRPVLAMLLVQLFATGMQLLSKLILNQGTFIFALMAYRHVVAALCVAPFAFFFDRS 73

Query: 69  NANKLSCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRM 128
           +ANK S QV FWLFLSA TGITAAMG+YYYGLRDTTATYATNFLNLIPVVTFVISS+L M
Sbjct: 74  HANKFSWQVLFWLFLSAFTGITAAMGMYYYGLRDTTATYATNFLNLIPVVTFVISSVLGM 133

Query: 129 EEVSFKRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLNISNYEPQPQP------ 188
           E+VS +RRAGK+KIVGAILCVGGALIT  YKGKGFHIGHH    I+ +     P      
Sbjct: 134 EKVSVRRRAGKVKIVGAILCVGGALITVFYKGKGFHIGHHA---IAGHRDVAAPAVIFNE 193

Query: 189 ----QPAHWGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGL 248
                 AHWGRGTLLL+GSCF YA WFVVQVKLLK+FPS YLATMLTCVIACIQST LGL
Sbjct: 194 IEADAEAHWGRGTLLLLGSCFCYAAWFVVQVKLLKLFPSKYLATMLTCVIACIQSTALGL 253

Query: 249 CLDTNKAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVAL 308
           CLD NKAAW L WDLQLLTIL SGAL TAATFCL+TWAISIQGPTFP MFNP++LI VA+
Sbjct: 254 CLDRNKAAWTLGWDLQLLTILYSGALATAATFCLMTWAISIQGPTFPPMFNPVTLILVAI 313

Query: 309 SEAIILGQEIRVG 312
           SE IILG+EIRVG
Sbjct: 314 SEGIILGEEIRVG 323

BLAST of Lag0020179.1 vs. ExPASy TrEMBL
Match: A0A6J1J9I9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.4e-126
Identity = 237/294 (80.61%), Postives = 263/294 (89.46%), Query Frame = 0

Query: 19  MLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQVF 78
           ML VQIF TG+QLLSKVILNHGTF+FALM+YRHLVAALCVAPFA FF+R+NANKLS QV 
Sbjct: 1   MLPVQIFATGMQLLSKVILNHGTFVFALMAYRHLVAALCVAPFA-FFERRNANKLSWQVL 60

Query: 79  FWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRRAG 138
           FWLFLSA TGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISS+LR+E+VS KRRAG
Sbjct: 61  FWLFLSAFTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSMLRIEKVSLKRRAG 120

Query: 139 KLKIVGAILCVGGALITSLYKGKGFHIGHHVG-LNISNYEPQPQPQPAHWGRGTLLLVGS 198
           ++ +VGAILCVGG +ITS+Y+GKGFHIGHHV  +N +           HWGRGTLLL+GS
Sbjct: 121 RITVVGAILCVGGVVITSVYRGKGFHIGHHVAHVNDNTNNNTSNEGGRHWGRGTLLLLGS 180

Query: 199 CFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLSWDLQLLT 258
           CFSY+ WFVVQVKLLK+FPS YLATMLTCVIACIQSTLLGLCLDTN A+WKL WDLQLLT
Sbjct: 181 CFSYSTWFVVQVKLLKLFPSKYLATMLTCVIACIQSTLLGLCLDTNNASWKLGWDLQLLT 240

Query: 259 ILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVG 312
           IL SGAL TAATFCL+TWAIS+QGPTFP MFNPL+LIFVA+SE IILG+EI+VG
Sbjct: 241 ILYSGALATAATFCLMTWAISMQGPTFPPMFNPLTLIFVAISEGIILGEEIKVG 293

BLAST of Lag0020179.1 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 171.8 bits (434), Expect = 8.8e-43
Identity = 105/308 (34.09%), Postives = 170/308 (55.19%), Query Frame = 0

Query: 14  RPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKL 73
           +P L + ++Q+  T + L+SK + N G   F  + YR   A + +AP A FF+RK+A  L
Sbjct: 7   KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPL 66

Query: 74  SCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSF 133
           S   F  +F+ +  G+T ++ L    L  T+AT A      +P +TF ++ +  ME +  
Sbjct: 67  SFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKV 126

Query: 134 KRRAGKLKIVGAILCVGGALITSLYKGKGFHIGHHVGLNISNYEPQPQP---QPAH---- 193
           K   G  K+VG  +C+GG +I ++YKG    +     L    Y  Q  P    P H    
Sbjct: 127 KSIQGTAKLVGITVCMGGVIILAIYKGPLLKL----PLCPHFYHGQEHPHRNNPGHVSGG 186

Query: 194 ---WGRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTN 253
              W +G +L++ S   + +W V+Q ++LKV+PS    T L C+++ IQS ++ + L+ +
Sbjct: 187 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 246

Query: 254 KAAWKLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAII 312
            +AWKL W+L+L+ ++  G + T   + L +W I  +GP F SMF PLSL+F  LS AI+
Sbjct: 247 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 306

BLAST of Lag0020179.1 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 96/300 (32.00%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 15  PVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLS 74
           P LAM+LVQI   G+ + SK+ +  G     L++YR + A +   P A F +RK   K++
Sbjct: 8   PFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKIT 67

Query: 75  CQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFK 134
            ++   +F  + TG T    LY+ GL++++ T A    NL+P VTF++++I R E V  K
Sbjct: 68  LRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIK 127

Query: 135 RRAGKLKIVGAILCVGGALITSLYKGKGFHIGH---HVGLNISNYEPQPQPQPAHWGRGT 194
           + +G+ K++G ++CV GA++ S Y G    IG    H     +  +       +++  G 
Sbjct: 128 KASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGHSNFFLGP 187

Query: 195 LLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLSW 254
            L++ +  S+A WF++Q K+ + F + Y +T+L C++  IQ   + L  D   + W LS 
Sbjct: 188 FLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLSS 247

Query: 255 DLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRVG 312
            L+ ++ L +G + +A  FCL++WA+  +GP + S+F+PL L+ VA+    +L +++  G
Sbjct: 248 PLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYTG 307

BLAST of Lag0020179.1 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 166.0 bits (419), Expect = 4.8e-41
Identity = 97/304 (31.91%), Postives = 169/304 (55.59%), Query Frame = 0

Query: 14  RPVLAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKL 73
           +P+LA++ +Q    G+ +++ V   HG   + L +YRH+VA + +APFA+ F+RK   K+
Sbjct: 10  KPILAIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRPKM 69

Query: 74  SCQVFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSF 133
           +  +F+ L             LYY GL++T+A+Y + F N +P VTF+++ I R+E V+F
Sbjct: 70  TLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETVNF 129

Query: 134 KRRAGKLKIVGAILCVGGALITSLYKG------KGFHIGHHVGLNISNYEPQPQPQPAHW 193
           ++     K+VG ++ VGGA+I +LYKG      K  H   H G + +       P   HW
Sbjct: 130 RKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIVKAAHNSFHGGSSST-------PTGQHW 189

Query: 194 GRGTLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAW 253
             GT+ ++GS  ++A +F++Q   LKV+P+      L C I  I + +  L +  + +AW
Sbjct: 190 VLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAW 249

Query: 254 KLSWDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQE 312
           K+  D   L  + SG + +   + + +  I  +GP F + F+P+ +I  A   A++L ++
Sbjct: 250 KIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEK 306

BLAST of Lag0020179.1 vs. TAIR 10
Match: AT1G75500.1 (Walls Are Thin 1 )

HSP 1 Score: 166.0 bits (419), Expect = 4.8e-41
Identity = 96/301 (31.89%), Postives = 155/301 (51.50%), Query Frame = 0

Query: 17  LAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQ 76
           +AML +Q    G  ++S+  LN G        YR+++A L + PFA F ++K    ++  
Sbjct: 22  IAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLN 81

Query: 77  VFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRR 136
                F  A  GITA  G Y  GL +T+ T+A++  N +P +TF+++++LR+E+V   RR
Sbjct: 82  FLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRR 141

Query: 137 AGKLKIVGAILCVGGALITSLYKGKGFHI------GHHVGLNISNYEPQPQPQPAHWGRG 196
            G  KI+G  LCV GA + +LYKG   +        H +  N +   P     P +W  G
Sbjct: 142 DGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLGNAAPKNWTLG 201

Query: 197 TLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLS 256
            + L+G C S++ W V Q  +LK +P+    T  TC    IQ  ++    + +  AW   
Sbjct: 202 CIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFH 261

Query: 257 WDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRV 312
              +L TIL +G + +   F +  W I   GP F +++ P+  + VA+  +I LG+E  +
Sbjct: 262 SGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYL 321

BLAST of Lag0020179.1 vs. TAIR 10
Match: AT1G75500.2 (Walls Are Thin 1 )

HSP 1 Score: 166.0 bits (419), Expect = 4.8e-41
Identity = 96/301 (31.89%), Postives = 155/301 (51.50%), Query Frame = 0

Query: 17  LAMLLVQIFITGIQLLSKVILNHGTFIFALMSYRHLVAALCVAPFAVFFQRKNANKLSCQ 76
           +AML +Q    G  ++S+  LN G        YR+++A L + PFA F ++K    ++  
Sbjct: 22  IAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEKKERPAITLN 81

Query: 77  VFFWLFLSASTGITAAMGLYYYGLRDTTATYATNFLNLIPVVTFVISSILRMEEVSFKRR 136
                F  A  GITA  G Y  GL +T+ T+A++  N +P +TF+++++LR+E+V   RR
Sbjct: 82  FLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALLRIEKVRINRR 141

Query: 137 AGKLKIVGAILCVGGALITSLYKGKGFHI------GHHVGLNISNYEPQPQPQPAHWGRG 196
            G  KI+G  LCV GA + +LYKG   +        H +  N +   P     P +W  G
Sbjct: 142 DGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLGNAAPKNWTLG 201

Query: 197 TLLLVGSCFSYAIWFVVQVKLLKVFPSTYLATMLTCVIACIQSTLLGLCLDTNKAAWKLS 256
            + L+G C S++ W V Q  +LK +P+    T  TC    IQ  ++    + +  AW   
Sbjct: 202 CIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFCERDSQAWVFH 261

Query: 257 WDLQLLTILCSGALGTAATFCLITWAISIQGPTFPSMFNPLSLIFVALSEAIILGQEIRV 312
              +L TIL +G + +   F +  W I   GP F +++ P+  + VA+  +I LG+E  +
Sbjct: 262 SGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYL 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877968.15.4e-13582.11WAT1-related protein At5g64700-like [Benincasa hispida][more]
KAG6576614.11.3e-13379.17WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG701466... [more]
XP_022922848.11.3e-13379.17WAT1-related protein At5g64700-like isoform X1 [Cucurbita moschata][more]
XP_023553116.11.9e-13278.53WAT1-related protein At5g64700-like [Cucurbita pepo subsp. pepo][more]
XP_022984779.12.5e-13279.17WAT1-related protein At5g64700-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FGG31.2e-4134.09WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
Q8GXB41.6e-4132.00WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q94AP36.8e-4031.89Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1[more]
Q9LPF16.8e-4031.91WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1... [more]
Q9ZUS12.0e-3931.89WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1E7Y36.4e-13479.17WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1[more]
A0A6J1JBI31.2e-13279.17WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1[more]
A0A6J1E4N23.4e-12780.61WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111430706 PE=3 SV=1[more]
A0A6J1CI517.6e-12777.96WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111011392 PE=3 SV=1[more]
A0A6J1J9I96.4e-12680.61WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482962 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64700.18.8e-4334.09nodulin MtN21 /EamA-like transporter family protein [more]
AT1G09380.11.1e-4232.00nodulin MtN21 /EamA-like transporter family protein [more]
AT1G44800.14.8e-4131.91nodulin MtN21 /EamA-like transporter family protein [more]
AT1G75500.14.8e-4131.89Walls Are Thin 1 [more]
AT1G75500.24.8e-4131.89Walls Are Thin 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 189..310
e-value: 4.0E-11
score: 43.2
coord: 15..156
e-value: 5.9E-14
score: 52.4
NoneNo IPR availablePANTHERPTHR31218:SF233WAT1-RELATED PROTEINcoord: 13..312
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 245..309
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 49..160
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 13..312

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0020179Lag0020179gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0020179.1.exon6Lag0020179.1.exon6exon
Lag0020179.1.exon5Lag0020179.1.exon5exon
Lag0020179.1.exon4Lag0020179.1.exon4exon
Lag0020179.1.exon3Lag0020179.1.exon3exon
Lag0020179.1.exon2Lag0020179.1.exon2exon
Lag0020179.1.exon1Lag0020179.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0020179.1cds.Lag0020179.1_6CDS
cds.Lag0020179.1cds.Lag0020179.1_5CDS
cds.Lag0020179.1cds.Lag0020179.1_4CDS
cds.Lag0020179.1cds.Lag0020179.1_3CDS
cds.Lag0020179.1cds.Lag0020179.1_2CDS
cds.Lag0020179.1cds.Lag0020179.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0020179.1Lag0020179.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity