Lag0020104.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020104.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cyclic nucleotide gated channel 1
Location	chr5: 48096509 .. 48097030 (+)
Sequence length	522
RNA-Seq Expression	Lag0020104.1
Synteny	Lag0020104.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTCAGCTCTCCTCAGAGGGTTGGCATCTAGTTCTGATGGAACTAGAAGCACTGAGAAGCTATTGAAATCTTCTTCCTCTAATAATCGCATGCCTGAAGGCTTGCGCTCAGGGAAGAAAATTCTTGATCCACAAGGGCCTTTTCTTCAAAGTTGGAACAAGATGTTTGTGCTGTCTTGTGTGATTGCTGTATCGTTGGATCCTCTGTTCTTTTATGTTCCTGTGATTGATAATAGAAGGAAGTGTCTTAGGTTAGATGAGAAAGTGGAGACTGTAGTCTGTATATTGCGATTATTTACAGACTTATTCTATGTTGTGCATATTGTTTTTCAGTTTCGTACAGGATTTATTGCTCCCTCTTCCCGAGTATTTGGAAGAGGTGTTTTAGTAGAAGATTATCGGGCGATAGCAATGAGGTATCTGTCATCATACTTTCTAATTGACATTTCTTCAGTCCTCCCACTTCCACAGGTGAGGAAAGTTTCTTGGGACTTGAGAGTATGTCTGCTCTACTGTTAA

mRNA sequence

ATGAATTCAGCTCTCCTCAGAGGGTTGGCATCTAGTTCTGATGGAACTAGAAGCACTGAGAAGCTATTGAAATCTTCTTCCTCTAATAATCGCATGCCTGAAGGCTTGCGCTCAGGGAAGAAAATTCTTGATCCACAAGGGCCTTTTCTTCAAAGTTGGAACAAGATGTTTGTGCTGTCTTGTGTGATTGCTGTATCGTTGGATCCTCTGTTCTTTTATGTTCCTGTGATTGATAATAGAAGGAAGTGTCTTAGGTTAGATGAGAAAGTGGAGACTGTAGTCTGTATATTGCGATTATTTACAGACTTATTCTATGTTGTGCATATTGTTTTTCAGTTTCGTACAGGATTTATTGCTCCCTCTTCCCGAGTATTTGGAAGAGGTGTTTTAGTAGAAGATTATCGGGCGATAGCAATGAGGTATCTGTCATCATACTTTCTAATTGACATTTCTTCAGTCCTCCCACTTCCACAGGTGAGGAAAGTTTCTTGGGACTTGAGAGTATGTCTGCTCTACTGTTAA

Coding sequence (CDS)

ATGAATTCAGCTCTCCTCAGAGGGTTGGCATCTAGTTCTGATGGAACTAGAAGCACTGAGAAGCTATTGAAATCTTCTTCCTCTAATAATCGCATGCCTGAAGGCTTGCGCTCAGGGAAGAAAATTCTTGATCCACAAGGGCCTTTTCTTCAAAGTTGGAACAAGATGTTTGTGCTGTCTTGTGTGATTGCTGTATCGTTGGATCCTCTGTTCTTTTATGTTCCTGTGATTGATAATAGAAGGAAGTGTCTTAGGTTAGATGAGAAAGTGGAGACTGTAGTCTGTATATTGCGATTATTTACAGACTTATTCTATGTTGTGCATATTGTTTTTCAGTTTCGTACAGGATTTATTGCTCCCTCTTCCCGAGTATTTGGAAGAGGTGTTTTAGTAGAAGATTATCGGGCGATAGCAATGAGGTATCTGTCATCATACTTTCTAATTGACATTTCTTCAGTCCTCCCACTTCCACAGGTGAGGAAAGTTTCTTGGGACTTGAGAGTATGTCTGCTCTACTGTTAA

Protein sequence

MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLSCVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAPSSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQVRKVSWDLRVCLLYC

Homology

[Close]

BLAST of Lag0020104.1 vs. NCBI nr
Match: XP_022945921.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022945922.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022945923.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLS 60
           MNSALLRGLASSS G R  EK LKSSS NN MP+GLRSGK+ILDPQGPFLQSWNK+FVLS
Sbjct: 1   MNSALLRGLASSSHGIRGIEKSLKSSSCNNLMPQGLRSGKRILDPQGPFLQSWNKIFVLS 60

Query: 61  CVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAP 120
           CV+AVSLDPLFFYVPVIDNR KCLRLDEKVETV CILRLFTDLFYVVHIVFQFRTGFIAP
Sbjct: 61  CVVAVSLDPLFFYVPVIDNRNKCLRLDEKVETVACILRLFTDLFYVVHIVFQFRTGFIAP 120

Query: 121 SSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           SSRVFGRGVL+ED  AIAMRYLSSYFLIDI SVLPLPQV
Sbjct: 121 SSRVFGRGVLIEDSWAIAMRYLSSYFLIDIFSVLPLPQV 159

[Close]

BLAST of Lag0020104.1 vs. NCBI nr
Match: KAG6574231.1 (Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLS 60
           MNSALLRGLASSS G R  EK LKSSS NN MP+GLRSGK+ILDPQGPFLQSWNK+FVLS
Sbjct: 1   MNSALLRGLASSSHGIRGIEKSLKSSSCNNLMPQGLRSGKRILDPQGPFLQSWNKIFVLS 60

Query: 61  CVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAP 120
           CV+AVSLDPLFFYVPVIDNR KCLRLDEKVETV CILRLFTDLFYVVHIVFQFRTGFIAP
Sbjct: 61  CVVAVSLDPLFFYVPVIDNRNKCLRLDEKVETVACILRLFTDLFYVVHIVFQFRTGFIAP 120

Query: 121 SSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           SSRVFGRGVL+ED  AIAMRYLSSYFLIDI SVLPLPQV
Sbjct: 121 SSRVFGRGVLIEDSWAIAMRYLSSYFLIDIFSVLPLPQV 159

[Close]

BLAST of Lag0020104.1 vs. NCBI nr
Match: KAG7013300.1 (Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLS 60
           MNSALLRGLASSS G R  EK LKSSS NN MP+GLRSGK+ILDPQGPFLQSWNK+FVLS
Sbjct: 1   MNSALLRGLASSSHGIRGIEKSLKSSSCNNLMPQGLRSGKRILDPQGPFLQSWNKIFVLS 60

Query: 61  CVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAP 120
           CV+AVSLDPLFFYVPVIDNR KCLRLDEKVETV CILRLFTDLFYVVHIVFQFRTGFIAP
Sbjct: 61  CVVAVSLDPLFFYVPVIDNRNKCLRLDEKVETVACILRLFTDLFYVVHIVFQFRTGFIAP 120

Query: 121 SSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           SSRVFGRGVL+ED  AIAMRYLSSYFLIDI SVLPLPQV
Sbjct: 121 SSRVFGRGVLIEDSWAIAMRYLSSYFLIDIFSVLPLPQV 159

[Close]

BLAST of Lag0020104.1 vs. NCBI nr
Match: XP_022945924.1 (cyclic nucleotide-gated ion channel 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLS 60
           MNSALLRGLASSS G R  EK LKSSS NN MP+GLRSGK+ILDPQGPFLQSWNK+FVLS
Sbjct: 1   MNSALLRGLASSSHGIRGIEKSLKSSSCNNLMPQGLRSGKRILDPQGPFLQSWNKIFVLS 60

Query: 61  CVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAP 120
           CV+AVSLDPLFFYVPVIDNR KCLRLDEKVETV CILRLFTDLFYVVHIVFQFRTGFIAP
Sbjct: 61  CVVAVSLDPLFFYVPVIDNRNKCLRLDEKVETVACILRLFTDLFYVVHIVFQFRTGFIAP 120

Query: 121 SSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           SSRVFGRGVL+ED  AIAMRYLSSYFLIDI SVLPLPQV
Sbjct: 121 SSRVFGRGVLIEDSWAIAMRYLSSYFLIDIFSVLPLPQV 159

[Close]

BLAST of Lag0020104.1 vs. NCBI nr
Match: XP_023541901.1 (cyclic nucleotide-gated ion channel 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 272.7 bits (696), Expect = 2.2e-69
Identity = 141/159 (88.68%), Postives = 146/159 (91.82%), Query Frame = 0


Query: 1   MNSALLRGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLS 60
           MNSAL RGLASSS G+R  EK LKS S NN MPEGLRSGK+ILDPQGPFLQSWNK+FVLS
Sbjct: 1   MNSALHRGLASSSHGSRGIEKSLKSPSCNNLMPEGLRSGKRILDPQGPFLQSWNKIFVLS 60

Query: 61  CVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAP 120
           CV+AVSLDPLFFYVPVIDNR KCLRLDEKVETV CILRLFTDLFYVVHIVFQFRTGFIAP
Sbjct: 61  CVVAVSLDPLFFYVPVIDNRNKCLRLDEKVETVACILRLFTDLFYVVHIVFQFRTGFIAP 120

Query: 121 SSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           SSRVFGRGVL+ED  AIAMRYLSSYFLIDI SVLPLPQV
Sbjct: 121 SSRVFGRGVLIEDSWAIAMRYLSSYFLIDIFSVLPLPQV 159

[Close]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.2e-49
Identity = 99/153 (64.71%), Postives = 122/153 (79.74%), Query Frame = 0


Query: 7   RGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLSCVIAVS 66
           R   SSS   +  ++  KS S    + +G+ S  KILDPQGPFLQ WNK+FVL+C+IAVS
Sbjct: 49  RSFESSSARIKLFKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVS 108

Query: 67  LDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAPSSRVFG 126
           LDPLFFYVP+ID+ +KCL +D+K+E    +LR FTD+FYV+HI+FQFRTGFIAPSSRVFG
Sbjct: 109 LDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFG 168

Query: 127 RGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           RGVLVED R IA RYLSS+F+IDI +VLPLPQ+
Sbjct: 169 RGVLVEDKREIAKRYLSSHFIIDILAVLPLPQM 201

[Close]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 3.4e-41
Identity = 73/120 (60.83%), Postives = 100/120 (83.33%), Query Frame = 0


Query: 40  KKILDPQGPFLQSWNKMFVLSCVIAVSLDPLFFYVPVIDNRRKCLRLDEKVETVVCILRL 99
           K I++PQ  FLQ+WNK+F+ +CV+A+++DPLFFY+P++D+ R CL LD K+E    +LR 
Sbjct: 66  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 125

Query: 100 FTDLFYVVHIVFQFRTGFIAPSSRVFGRGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 159
             D FY++HIVFQFRT +IAPSSRVFGRG LV+D +AIA++YLSSYF+ID+ S+LPLPQ+
Sbjct: 126 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 185

[Close]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 4.1e-39
Identity = 77/145 (53.10%), Postives = 106/145 (73.10%), Query Frame = 0


Query: 17  RSTEKLLKSSSSNNRMP--EGLRSGKKILDPQGPFLQSWNKMFVLSCVIAVSLDPLFFYV 76
           R  +K L   S N +        + K I++PQG FLQ+WNK+F+ + VIA+++DPLFFY+
Sbjct: 41  RGLKKPLSFGSHNKKRDSNSSTTTQKNIINPQGSFLQNWNKIFLFASVIALAIDPLFFYI 100

Query: 77  PVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAPSSRVFGRGVLVEDY 136
           P++D  R CL L   +E    +LR F D FY++HIVFQFRT +I+PSSRVFGRG LV+D 
Sbjct: 101 PIVDGERHCLNLHRNLEIAASVLRTFIDAFYIIHIVFQFRTAYISPSSRVFGRGELVDDP 160

Query: 137 RAIAMRYLSSYFLIDISSVLPLPQV 160
           +AIA++YLSSYF+ID+ S+LPLPQ+
Sbjct: 161 KAIAIKYLSSYFIIDLLSILPLPQL 185

[Close]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-36
Identity = 73/153 (47.71%), Postives = 105/153 (68.63%), Query Frame = 0


Query: 7   RGLASSSDGTRSTEKLLKSSSSNNRMPEGLRSGKKILDPQGPFLQSWNKMFVLSCVIAVS 66
           R     S+  R+ ++ L   S+ N+     +   ++++P   +LQSWNK+F+L  V+A++
Sbjct: 37  RSFEKGSEKIRTFKRPLSVHSNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALA 96

Query: 67  LDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAPSSRVFG 126
            DPLFFY+P +   R CL LD+K++T+ C+ R F D FYVVH++FQF TGFI PSS  FG
Sbjct: 97  FDPLFFYIPYVKPERFCLNLDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFG 156

Query: 127 RGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           RG L E ++ IA+RYL SYFLID+ S+LP+PQV
Sbjct: 157 RGELNEKHKDIALRYLGSYFLIDLLSILPIPQV 189

[Close]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot
Match: Q9FXH6 (Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 GN=CNGC8 PE=3 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-35
Identity = 76/153 (49.67%), Postives = 102/153 (66.67%), Query Frame = 0


Query: 8   GLASSSDGTRSTEKLLKSSSSNNRMPEGLR-SGKKILDPQGPFLQSWNKMFVLSCVIAVS 67
           G+   S+G ++  K   S  +    PE LR S KKI DPQ   L  WN+MFV+SC++AVS
Sbjct: 62  GVKIGSEGLKTIGKSFTSGVTRAVFPEDLRVSEKKIFDPQDKTLLLWNRMFVISCILAVS 121

Query: 68  LDPLFFYVPVIDNRRKCLRLDEKVETVVCILRLFTDLFYVVHIVFQFRTGFIAPSSRVFG 127
           +DPLFFY+P++DN + C+ +D K+      LR   D+FY+  +  QFRT +IAPSSRVFG
Sbjct: 122 VDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTIIDVFYLTRMALQFRTAYIAPSSRVFG 181

Query: 128 RGVLVEDYRAIAMRYLSSYFLIDISSVLPLPQV 160
           RG LV D   IA RYL+ YF++D  +VLPLPQ+
Sbjct: 182 RGELVIDPAKIAERYLTRYFIVDFLAVLPLPQI 214

The following BLAST results are available for this feature:

BLAST of Lag0020104.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945921.1	3.3e-70	89.31	cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_0...	[more]
KAG6574231.1	3.3e-70	89.31	Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7013300.1	3.3e-70	89.31	Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022945924.1	3.3e-70	89.31	cyclic nucleotide-gated ion channel 1-like isoform X2 [Cucurbita moschata]	[more]
XP_023541901.1	2.2e-69	88.68	cyclic nucleotide-gated ion channel 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0020104.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65717	1.2e-49	64.71	Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 P...	[more]
Q9LNJ0	3.4e-41	60.83	Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LD40	4.1e-39	53.10	Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SKD7	2.5e-36	47.71	Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FXH6	1.2e-35	49.67	Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 G...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005821	Ion transport domain	PFAM	PF00520	Ion_trans	coord: 51..158 e-value: 1.1E-9 score: 37.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..39
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	PANTHER	PTHR45651	CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED	coord: 4..160
None	No IPR available	PANTHER	PTHR45651:SF91	CYCLIC NUCLEOTIDE-GATED ION CHANNEL-LIKE PROTEIN	coord: 4..160
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 44..161

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0020104	Lag0020104	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0020104.1.exon1	Lag0020104.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0020104.1	cds.Lag0020104.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0020104.1	Lag0020104.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006811	ion transport
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0034220	ion transmembrane transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005216	ion channel activity
molecular_function	GO:0030552	cAMP binding
molecular_function	GO:0030553	cGMP binding