Lag0018561.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0018561.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2S albumin
Location	chr5: 29536896 .. 29537321 (-)
Sequence length	426
RNA-Seq Expression	Lag0018561.1
Synteny	Lag0018561.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAGACTCACAGGAATCATTGCCCTCTTGGCTGTGGCGCTGCTGGTTGCAGATGCCTACGCCTACCGCACCACCATCACCACCGTCGAGGTAGACGAAGACAACCAAGGGCGGCAGCAGAGGTGCCGGCAGATGAGGGCTCGCCAGGAGCTCCGCAGCTGCGAGCAGTACCTGAGGCAGCAACAGAGCAGAGATGTCTTGCAGATGCGAGGAATCGACAACCCAAGGAGAGGAGAGCAGGCCTTCGATGAGTGCTGCAGAGAGTTGAGGAATGTGGATGAGCAGTGCAGGTGCGATCTCTTGGAGGAGATTGCTAGGGAGGAGCAGAGGCAAGCACGAGGCCAAGAAGGAAGGCAGATGCTACAGAGAGCTAGGGACTTGCCATCCATGTGCGGAATCCGCCAACAGCGATGCGACTTCTAA

mRNA sequence

ATGGCCAGACTCACAGGAATCATTGCCCTCTTGGCTGTGGCGCTGCTGGTTGCAGATGCCTACGCCTACCGCACCACCATCACCACCGTCGAGGTAGACGAAGACAACCAAGGGCGGCAGCAGAGGTGCCGGCAGATGAGGGCTCGCCAGGAGCTCCGCAGCTGCGAGCAGTACCTGAGGCAGCAACAGAGCAGAGATGTCTTGCAGATGCGAGGAATCGACAACCCAAGGAGAGGAGAGCAGGCCTTCGATGAGTGCTGCAGAGAGTTGAGGAATGTGGATGAGCAGTGCAGGTGCGATCTCTTGGAGGAGATTGCTAGGGAGGAGCAGAGGCAAGCACGAGGCCAAGAAGGAAGGCAGATGCTACAGAGAGCTAGGGACTTGCCATCCATGTGCGGAATCCGCCAACAGCGATGCGACTTCTAA

Coding sequence (CDS)

ATGGCCAGACTCACAGGAATCATTGCCCTCTTGGCTGTGGCGCTGCTGGTTGCAGATGCCTACGCCTACCGCACCACCATCACCACCGTCGAGGTAGACGAAGACAACCAAGGGCGGCAGCAGAGGTGCCGGCAGATGAGGGCTCGCCAGGAGCTCCGCAGCTGCGAGCAGTACCTGAGGCAGCAACAGAGCAGAGATGTCTTGCAGATGCGAGGAATCGACAACCCAAGGAGAGGAGAGCAGGCCTTCGATGAGTGCTGCAGAGAGTTGAGGAATGTGGATGAGCAGTGCAGGTGCGATCTCTTGGAGGAGATTGCTAGGGAGGAGCAGAGGCAAGCACGAGGCCAAGAAGGAAGGCAGATGCTACAGAGAGCTAGGGACTTGCCATCCATGTGCGGAATCCGCCAACAGCGATGCGACTTCTAA

Protein sequence

MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLRQQQSRDVLQMRGIDNPRRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGRQMLQRARDLPSMCGIRQQRCDF

Homology

[Close]

BLAST of Lag0018561.1 vs. NCBI nr
Match: Q39649.1 (RecName: Full=2S albumin; Contains: RecName: Full=2S albumin small chain; Contains: RecName: Full=2S albumin large chain; Flags: Precursor [Cucurbita maxima] >pir|T10257| 2S albumin precursor - cucurbit >BAA03993.1 prepro2S albumin [Cucurbita hybrid cultivar])

HSP 1 Score: 221.1 bits (562), Expect = 6.1e-54
Identity = 120/142 (84.51%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLT IIAL AVALLVADAYAYRTTITTVEV+E+ QGR++RCRQM AR+ELRSCEQYLR
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRQGREERCRQMSAREELRSCEQYLR 60

Query: 61  QQQSRDVLQMRGIDNP-RRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSRDVLQMRGI+NP RR   +FDECCREL+NVDE+CRCD+LEEIAREEQRQARGQEGR
Sbjct: 61  -QQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           QMLQ+AR+LPSMCGIR QRCDF
Sbjct: 121 QMLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lag0018561.1 vs. NCBI nr
Match: XP_022993226.1 (2S albumin [Cucurbita maxima])

HSP 1 Score: 220.7 bits (561), Expect = 8.0e-54
Identity = 120/142 (84.51%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLT IIAL AVALLVADAYAYRTTITTVEV+E+ QGR++RCRQM AR+ELRSCE+YLR
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRQGREERCRQMSAREELRSCEKYLR 60

Query: 61  QQQSRDVLQMRGIDNP-RRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSRDVLQMRGI+NP RR   +FDECCRELRNVDE+CRCD+LEEIAREEQRQARGQEGR
Sbjct: 61  -QQSRDVLQMRGIENPWRREGGSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           QMLQ+AR+LPSMCGIR QRCDF
Sbjct: 121 QMLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lag0018561.1 vs. NCBI nr
Match: AJO68010.1 (alpha-amylase inhibitor [Trichosanthes kirilowii])

HSP 1 Score: 220.7 bits (561), Expect = 8.0e-54
Identity = 121/142 (85.21%), Postives = 130/142 (91.55%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLTGIIALLAVALLV +AYAYRTTITTVEVD+DNQGRQQRCRQM AR++LRSCEQYLR
Sbjct: 1   MARLTGIIALLAVALLVVEAYAYRTTITTVEVDDDNQGRQQRCRQMSAREKLRSCEQYLR 60

Query: 61  QQQSRDVLQMRGIDNPRRGE-QAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSRDVL MRGIDNPRR E  +FDECC ELRNVDEQCRCDLLEEIAREEQRQARGQEGR
Sbjct: 61  -QQSRDVLLMRGIDNPRRKEGGSFDECCSELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           Q+L +AR+LPSMCG+R  RC F
Sbjct: 121 QLLDKARNLPSMCGLRPDRCAF 141

[Close]

BLAST of Lag0018561.1 vs. NCBI nr
Match: XP_022942593.1 (2S albumin [Cucurbita moschata])

HSP 1 Score: 217.6 bits (553), Expect = 6.8e-53
Identity = 118/142 (83.10%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLT IIAL AVALLVADAYAYRTTITTVEV+E+ +G ++RCRQM AR+ELRSCEQYLR
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRRGGEERCRQMSAREELRSCEQYLR 60

Query: 61  QQQSRDVLQMRGIDNP-RRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSR+VLQMRGI+NP RR  ++FDECCRELRNVDE+CRCD+LEEIAREEQRQARGQEGR
Sbjct: 61  -QQSREVLQMRGIENPWRREGRSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           QMLQ+AR+LPSMCGIR QRCDF
Sbjct: 121 QMLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lag0018561.1 vs. NCBI nr
Match: XP_023545481.1 (2S albumin [Cucurbita pepo subsp. pepo] >KAG7031398.1 hypothetical protein SDJN02_05438, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 216.5 bits (550), Expect = 1.5e-52
Identity = 118/142 (83.10%), Postives = 131/142 (92.25%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLT IIAL AVALLVADAYAYRTTITTVEV+E+ +G ++RCRQM AR+ELRSCEQYLR
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENKRGGEERCRQMSAREELRSCEQYLR 60

Query: 61  QQQSRDVLQMRGIDNP-RRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSR+VLQMRGI+NP RR   +FDECCRELRNVDE+CRCD+LEEIAREEQRQARGQEGR
Sbjct: 61  -QQSREVLQMRGIENPWRREGGSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           QMLQ+AR+LPSMCGIR QRCDF
Sbjct: 121 QMLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot
Match: Q39649 (2S albumin OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 8.0e-57
Identity = 120/142 (84.51%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLR 60
           MARLT IIAL AVALLVADAYAYRTTITTVEV+E+ QGR++RCRQM AR+ELRSCEQYLR
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRQGREERCRQMSAREELRSCEQYLR 60

Query: 61  QQQSRDVLQMRGIDNP-RRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGR 120
            QQSRDVLQMRGI+NP RR   +FDECCREL+NVDE+CRCD+LEEIAREEQRQARGQEGR
Sbjct: 61  -QQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGR 120

Query: 121 QMLQRARDLPSMCGIRQQRCDF 142
           QMLQ+AR+LPSMCGIR QRCDF
Sbjct: 121 QMLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot
Match: P93198 (2S albumin seed storage protein (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 6.6e-19
Identity = 62/138 (44.93%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 9   ALLAVALLVADAYAYRTTITTVEVDEDNQGRQQR---CR-QMRARQELRSCEQYLRQQQS 68
           ALL   L VA+A A+RTTITT+E+DED    ++R   CR Q++ +Q L  C+ YLRQQ  
Sbjct: 2   ALLVALLFVANAAAFRTTITTMEIDEDIDNPRRRGEGCREQIQRQQNLNHCQYYLRQQS- 61

Query: 69  RDVLQMRGIDNPRRGEQAFDECCRELRNVDEQCRCDLLEEIAREEQRQ--ARGQEGRQML 128
               +  G D   +  Q F +CC++L  +DEQC+C+ L ++ R +Q+Q   RG+E  +M+
Sbjct: 62  ----RSGGYDEDNQ-RQHFRQCCQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMV 121

Query: 129 QRARDLPSMCGIRQQRCD 141
           Q ARDLP+ CGI  QRC+
Sbjct: 122 QSARDLPNECGISSQRCE 133

[Close]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot
Match: P04403 (2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa OX=3645 GN=BE2S1 PE=1 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-13
Identity = 54/144 (37.50%), Postives = 82/144 (56.94%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCR-QMRARQELRSCEQYL 60
           MA+++   A L V + +  A A+R T+TT  V+E+N   Q+ CR QM+ +Q L  C  Y+
Sbjct: 1   MAKISVAAAALLVLMALGHATAFRATVTTTVVEEEN---QEECREQMQRQQMLSHCRMYM 60

Query: 61  RQQQSRDVLQMRGIDNPRRG-EQAFDECCRELRNVDEQCRCDLLEEI---AREEQRQARG 120
           RQQ      Q      PRRG E    ECC +L  +DE CRC+ L  +    ++E+ Q RG
Sbjct: 61  RQQMEESPYQTM----PRRGMEPHMSECCEQLEGMDESCRCEGLRMMMMRMQQEEMQPRG 120

Query: 121 QEGRQMLQRARDLPSMCGIRQQRC 140
           ++ R+M++ A ++PS C +   RC
Sbjct: 121 EQMRRMMRLAENIPSRCNLSPMRC 137

[Close]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot
Match: P0C8Y8 (2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa OX=3645 GN=BE2S2 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-13
Identity = 57/150 (38.00%), Postives = 90/150 (60.00%), Query Frame = 0


Query: 1   MARLTGIIALLAVALLVADAYAYRTTITTV---EVDEDNQGR-QQRCR-QMRARQELRSC 60
           MA+++ + A L   L++  A A+RTT+TT    E +E+ +GR +Q+CR QM  +Q+L  C
Sbjct: 1   MAKMSVVAAALLALLVLGQATAFRTTVTTTLEEEQEENPRGRSEQQCREQMERQQQLNHC 60

Query: 61  EQYLRQQQSRDVLQMRGIDNP---RRGEQA-FDECCRELRNVDEQCRCDLLEEIAR--EE 120
             YLRQQ      Q     NP   RRGE+   DECC +L  +DE CRC+ L  + R   E
Sbjct: 61  RMYLRQQMEESPYQ-----NPRPLRRGEEPHLDECCEQLERMDEMCRCEGLRMMLRRQRE 120

Query: 121 QRQARGQEGRQMLQRARDLPSMCGIRQQRC 140
           + + +G++ ++++++A +L S C +  QRC
Sbjct: 121 EMELQGEQMQRIMRKAENLLSRCNLSPQRC 145

[Close]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot
Match: P15461 (2S seed storage protein OS=Helianthus annuus OX=4232 GN=HAG5 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.2e-13
Identity = 53/149 (35.57%), Postives = 86/149 (57.72%), Query Frame = 0


Query: 7   IIALLAVALLVADAYAYRTTITTVEVDEDNQGRQQRCRQMRARQELRSCEQYLRQQQSRD 66
           I+  LA A LVA A A+ T ITT   DE+    Q++  Q    Q L  C  +L+Q Q+  
Sbjct: 5   IVLALAFAALVAFATAHTTIITTTIEDENPISGQRQVSQRIQGQRLNQCRMFLQQGQNIP 64

Query: 67  VLQMRGIDNPRRG---EQAFDECCRELRNVDEQCRCDLLEEIAREEQRQARGQEGRQML- 126
               R  DNP+ G   EQ   +CC+EL+N++ QC+C+ ++++ RE Q+Q + Q+GRQ++ 
Sbjct: 65  ----REFDNPQMGRQQEQQLQQCCQELQNIEGQCQCEAVKQVFREAQQQVQQQQGRQLVP 124

Query: 127 -----------QRARDLPSMCGIRQQRCD 141
                      Q+A+ LP++C ++ +RC+
Sbjct: 125 FRGSQQTQQLKQKAQILPNVCNLQSRRCE 149

The following BLAST results are available for this feature:

BLAST of Lag0018561.1 vs. NCBI nr


Match Name	E-value	Identity	Description
Q39649.1	6.1e-54	84.51	RecName: Full=2S albumin; Contains: RecName: Full=2S albumin small chain; Contai...	[more]
XP_022993226.1	8.0e-54	84.51	2S albumin [Cucurbita maxima]	[more]
AJO68010.1	8.0e-54	85.21	alpha-amylase inhibitor [Trichosanthes kirilowii]	[more]
XP_022942593.1	6.8e-53	83.10	2S albumin [Cucurbita moschata]	[more]
XP_023545481.1	1.5e-52	83.10	2S albumin [Cucurbita pepo subsp. pepo] >KAG7031398.1 hypothetical protein SDJN0...	[more]

BLAST of Lag0018561.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39649	8.0e-57	84.51	2S albumin OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P93198	6.6e-19	44.93	2S albumin seed storage protein (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1	[more]
P04403	1.9e-13	37.50	2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa OX=3645 GN=BE2S1 P...	[more]
P0C8Y8	1.9e-13	38.00	2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa OX=3645 GN=BE2S2 P...	[more]
P15461	3.2e-13	35.57	2S seed storage protein OS=Helianthus annuus OX=4232 GN=HAG5 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000617	Napin/ Bra allergen	PRINTS	PR00496	NAPIN	coord: 41..62 score: 30.68 coord: 118..132 score: 34.0 coord: 85..102 score: 40.28
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 55..139 e-value: 6.9E-10 score: 48.9
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF00234	Tryp_alpha_amyl	coord: 55..139 e-value: 1.8E-9 score: 37.9
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 36..141 e-value: 5.6E-22 score: 80.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 9..140
None	No IPR available	PANTHER	PTHR35496	2S SEED STORAGE PROTEIN 1-RELATED	coord: 1..141
IPR044723	AAI/SS protein, conserved domain	CDD	cd00261	AAI_SS	coord: 40..141 e-value: 5.89924E-11 score: 53.9459

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0018561	Lag0018561	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0018561.1.exon1	Lag0018561.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0018561.1	cds.Lag0018561.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0018561.1	Lag0018561.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0033095	aleurone grain
cellular_component	GO:0000322	storage vacuole
molecular_function	GO:0045735	nutrient reservoir activity
molecular_function	GO:0015066	alpha-amylase inhibitor activity