Lag0017892.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0017892.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr5: 10980231 .. 10981387 (-)
Sequence length1056
RNA-Seq ExpressionLag0017892.1
SyntenyLag0017892.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTTTGCAGTATTCCTCTTGGGCGGGCAGCGGCGGTTGACTTGTGGTTCTGGCATTATGAGAAGAATGGGTGCTATAGCGTGCGTAGTGGTTACAAGTTGTGTATGTTGTTGCGCGGCTCAACTTCTTCAGCTTCCTCTCACGATTCTCGCTAGTGGTGGTCTGTTTTGTGGAAGTTGCCCTTGTCGCCTAAAGTCAAGGTGTTTATGTGGCGGGCTTTCCATTCTCTTCTTCCTGTTATGACGTCGCTGCTTCAACGCGGTATGGATGTTTCTCCTCTATGTTCTTTGTGTACTGATAGTTATGAATCCACAGACCACCCGCTGGTGGGTTGTGAGCGAGCTTTGCTTTTTTGGAAACGTGTGTTGCCGGCGGTTCACTGGGAGGCTGACTTTGGCCAAAGTTTTCTAGATCGGTGTTTGTTTTTGTTTTCCACCCTCTCTGATCGTGATCTTATAATGTGGTGTGTGGGTTGTTGGGCCTTATGGAATGATAGGAATACAGTTCGAGTTGGGCGAAAAATCTCAGATTTGATAGCGAAATGTAATTGGGTTGAAGCATACATTGACTCTTTCCGACTTGTTCGTGCATCATGTCGATCGGGGGCTTCGTCTGTGGGGGTTGTTTCGCCAGTTCCCCATTGGATTCGGCCGCCGGTGGGCTATGTTAAGCTGAACGTGGATGCTTCTGTTTCTATGGATCGACGACAATCAGGCATTGGGGTTCTTGTTCGTGATTGCTACGGTTCTGTGGTGGCTGCTATGGTTAACTCTTTTGCGGTATCTTATTCTCCCTTCAATGTTGAAGCTTTGGCAGTATTGGAGGGGTTGTATTTGGCTCTGCGACTCAACCTATCCTTGATTATTGTTGAGTCGGATTGCAAGGAGCTGGTCGACATGTTGAATGGGGTCACCTCCACTTTGTTTGAGGTCGGTATTTGGCCGGTCACAGATATTCAGTCTTTGGTTCCTAAGTTTGATTGTATTTCCTTCTCTCACGTGGGGCGTGGGTGTAATGTGCCTGCCCATTGTCTTGCGAGGAGGGGACTGTCTATTGGCTCTATTCTTTGGCTTGCTGAATTTCCAGCTTGGTCGCTGAGTATTCTCAAGGTTGATAATGCCTTGGTTGTATCTGGCCCATGTGGTCTTTCTTAA

mRNA sequence

ATGATGTTTGCAGTATTCCTCTTGGGCGGGCAGCGGCGGTTGACTTGTGGTTCTGGCATTATGAGAAGAATGGGTGCTATAGCGTGCGTAGTGGTTACAAGTTGTGTATGTTGTTGCGCGGCTCAACTTCTTCAGCTTCCTCTCACGATTCTCGCTAGTGGTGGTCTGTTTTGTGGAAGTTGCCCTTGTCGCCTAAAGTCAAGTTATGAATCCACAGACCACCCGCTGGTGGGTTGTGAGCGAGCTTTGCTTTTTTGGAAACGTGTGTTGCCGGCGGTTCACTGGGAGGCTGACTTTGGCCAAAGTTTTCTAGATCGGTGTTTGTTTTTGTTTTCCACCCTCTCTGATCGTGATCTTATAATGTGGTGTGTGGGTTGTTGGGCCTTATGGAATGATAGGAATACAGTTCGAGTTGGGCGAAAAATCTCAGATTTGATAGCGAAATGTAATTGGGTTGAAGCATACATTGACTCTTTCCGACTTGTTCGTGCATCATGTCGATCGGGGGCTTCGTCTGTGGGGGTTGTTTCGCCAGTTCCCCATTGGATTCGGCCGCCGGTGGGCTATGTTAAGCTGAACGTGGATGCTTCTGTTTCTATGGATCGACGACAATCAGGCATTGGGGTTCTTGTTCGTGATTGCTACGGTTCTGTGGTGGCTGCTATGGTTAACTCTTTTGCGGTATCTTATTCTCCCTTCAATGTTGAAGCTTTGGCAGTATTGGAGGGGTTGTATTTGGCTCTGCGACTCAACCTATCCTTGATTATTGTTGAGTCGGATTGCAAGGAGCTGGTCGACATGTTGAATGGGGTCACCTCCACTTTGTTTGAGGTCGGTATTTGGCCGGTCACAGATATTCAGTCTTTGGTTCCTAAGTTTGATTGTATTTCCTTCTCTCACGTGGGGCGTGGGTGTAATGTGCCTGCCCATTGTCTTGCGAGGAGGGGACTGTCTATTGGCTCTATTCTTTGGCTTGCTGAATTTCCAGCTTGGTCGCTGAGTATTCTCAAGGTTGATAATGCCTTGGTTGTATCTGGCCCATGTGGTCTTTCTTAA

Coding sequence (CDS)

ATGATGTTTGCAGTATTCCTCTTGGGCGGGCAGCGGCGGTTGACTTGTGGTTCTGGCATTATGAGAAGAATGGGTGCTATAGCGTGCGTAGTGGTTACAAGTTGTGTATGTTGTTGCGCGGCTCAACTTCTTCAGCTTCCTCTCACGATTCTCGCTAGTGGTGGTCTGTTTTGTGGAAGTTGCCCTTGTCGCCTAAAGTCAAGTTATGAATCCACAGACCACCCGCTGGTGGGTTGTGAGCGAGCTTTGCTTTTTTGGAAACGTGTGTTGCCGGCGGTTCACTGGGAGGCTGACTTTGGCCAAAGTTTTCTAGATCGGTGTTTGTTTTTGTTTTCCACCCTCTCTGATCGTGATCTTATAATGTGGTGTGTGGGTTGTTGGGCCTTATGGAATGATAGGAATACAGTTCGAGTTGGGCGAAAAATCTCAGATTTGATAGCGAAATGTAATTGGGTTGAAGCATACATTGACTCTTTCCGACTTGTTCGTGCATCATGTCGATCGGGGGCTTCGTCTGTGGGGGTTGTTTCGCCAGTTCCCCATTGGATTCGGCCGCCGGTGGGCTATGTTAAGCTGAACGTGGATGCTTCTGTTTCTATGGATCGACGACAATCAGGCATTGGGGTTCTTGTTCGTGATTGCTACGGTTCTGTGGTGGCTGCTATGGTTAACTCTTTTGCGGTATCTTATTCTCCCTTCAATGTTGAAGCTTTGGCAGTATTGGAGGGGTTGTATTTGGCTCTGCGACTCAACCTATCCTTGATTATTGTTGAGTCGGATTGCAAGGAGCTGGTCGACATGTTGAATGGGGTCACCTCCACTTTGTTTGAGGTCGGTATTTGGCCGGTCACAGATATTCAGTCTTTGGTTCCTAAGTTTGATTGTATTTCCTTCTCTCACGTGGGGCGTGGGTGTAATGTGCCTGCCCATTGTCTTGCGAGGAGGGGACTGTCTATTGGCTCTATTCTTTGGCTTGCTGAATTTCCAGCTTGGTCGCTGAGTATTCTCAAGGTTGATAATGCCTTGGTTGTATCTGGCCCATGTGGTCTTTCTTAA

Protein sequence

MMFAVFLLGGQRRLTCGSGIMRRMGAIACVVVTSCVCCCAAQLLQLPLTILASGGLFCGSCPCRLKSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHVGRGCNVPAHCLARRGLSIGSILWLAEFPAWSLSILKVDNALVVSGPCGLS
Homology
BLAST of Lag0017892.1 vs. NCBI nr
Match: XP_024046732.1 (uncharacterized protein LOC112101057 [Citrus clementina])

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-28
Identity = 87/268 (32.46%), Postives = 139/268 (51.87%), Query Frame = 0

Query: 66  KSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMWCVG 125
           KS  E   H  + C+ A   WK    A   +    +  L     L   +S R++    V 
Sbjct: 117 KSGVEDAFHAFMECKVARKVWKYSYLASEMQEVVREGILSVMQSLIKKMSMREVEFVAVV 176

Query: 126 CWALWNDRN-TVRVGRKIS--DLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPHW 185
            W +W+ RN  +  G++++   LIAK    +A +++++ V    ++   S   VS    W
Sbjct: 177 WWEIWHARNKLIFEGKRVNPMSLIAK---AQAAVEAYQRVHGKEQTSKRSED-VSKQDQW 236

Query: 186 IRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVLE 245
             PPVG+ K+NVDA+V  +++ +G+GV++R+  G V+   V S     +    EA AV  
Sbjct: 237 CPPPVGFYKVNVDAAVHTEQQLTGLGVVIRNPQGQVIVTAVKSTKFQDNVTAAEAEAVKW 296

Query: 246 GLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHVG 305
           GL +AL   L+ +I+E+DC E+ ++ N  TS+  E+ +W ++DI S   KF  +S  HV 
Sbjct: 297 GLEIALEARLAAVIIETDCIEVANLANSKTSSRKEI-VWTISDIHSYKEKFQSLSIQHVP 356

Query: 306 RGCNVPAHCLARRGL-SIGSILWLAEFP 330
           R CN  AH LA+R L S  ++ W   FP
Sbjct: 357 RCCNSCAHSLAKRALRSSETVTWKDNFP 379

BLAST of Lag0017892.1 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-27
Identity = 88/278 (31.65%), Postives = 137/278 (49.28%), Query Frame = 0

Query: 64   RLKSSYESTDHPLVGCERALLFW-KRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMW 123
            R K S E+  H L+ C+ A   W +    A   EA+  Q        +   L   DL + 
Sbjct: 1117 RCKLSVETISHALLECKAARKIWLQSPFSAPRLEAN-SQDIFSTLQNMAKELRKSDLELM 1176

Query: 124  CVGCWALWNDRN-TVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPH 183
               CW+ W  RN  +  GR+++ +I+     E+ + +F+ VR   +S   S+ +      
Sbjct: 1177 VALCWSAWYARNKCIFDGRELNPIISAAK-AESVLTAFQRVRKPQQSHI-SISIKEKQQE 1236

Query: 184  WIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVL 243
            W+ PP    K+NVDA+ +     +G+G ++RD  G +VAA VN   +  S    EA AVL
Sbjct: 1237 WLPPPQNVFKVNVDAAFNSKNLSAGVGAVIRDSNGKIVAAGVNQNLLKGSASLAEAEAVL 1296

Query: 244  EGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHV 303
             GL LA   ++S +I+ESDC E+V ++N    +  E+  W +  IQ+ +  F  +  +H+
Sbjct: 1297 WGLQLARNADVSSLIIESDCLEVVQLVNNTKGSRSEI-FWTILAIQNQMKIFQKVVVNHI 1356

Query: 304  GRGCNVPAHCLARRGL-SIGSILWLAEFPAWSLSILKV 339
             R CN  AH LA+  L  I   +WL   PA     +KV
Sbjct: 1357 PRHCNACAHYLAKIALGKISPCMWLGNIPAELYDFIKV 1390

BLAST of Lag0017892.1 vs. NCBI nr
Match: XP_024033146.1 (uncharacterized protein LOC112095446 [Citrus clementina])

HSP 1 Score: 133.3 bits (334), Expect = 4.2e-27
Identity = 83/255 (32.55%), Postives = 136/255 (53.33%), Query Frame = 0

Query: 79  CERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMWCVGCWALWNDRN-TVR 138
           C+ A   WK    A   +    +  L     L   +S R++    V  W +W+ RN  + 
Sbjct: 3   CKVARKVWKYSYLASEMQGVVREDILSVMQSLIKKMSMREVEFVAVVWWEIWHARNKLIF 62

Query: 139 VGRKIS--DLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPHWIRPPVGYVKLNVD 198
            G++++   LIAK    +A +++++ V    ++   S   VS    W  PPVG+ K+NVD
Sbjct: 63  EGKRVNPMSLIAK---AQAAVEAYQRVHGKEQTSKRSED-VSKQDQWFPPPVGFYKVNVD 122

Query: 199 ASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVLEGLYLALRLNLSLI 258
           A+V  +++ +G+GV++R+  G ++ A V S     +    EA AV  GL +AL   L+ +
Sbjct: 123 AAVHTEQQLTGLGVVIRNPQGQIIVAAVKSTKFQDNVTAAEAEAVKWGLEIALEARLATV 182

Query: 259 IVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHVGRGCNVPAHCLARR 318
           I+E+DC E+ ++ N  TS+  E+ +W ++DI S   KF  +S  HV R CN  AH LA+R
Sbjct: 183 IIETDCIEVANLANSKTSSRKEI-MWTISDIHSYKEKFQSLSIQHVPRCCNSCAHSLAKR 242

Query: 319 GL-SIGSILWLAEFP 330
            L S+ ++ W   FP
Sbjct: 243 ALRSLETVTWKDNFP 252

BLAST of Lag0017892.1 vs. NCBI nr
Match: XP_015385025.1 (uncharacterized protein LOC107176703 [Citrus sinensis])

HSP 1 Score: 132.1 bits (331), Expect = 9.3e-27
Identity = 82/255 (32.16%), Postives = 136/255 (53.33%), Query Frame = 0

Query: 79  CERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMWCVGCWALWNDRN-TVR 138
           C+ A   WK    A   +    +  L     L   +S R++    V  W +W+ RN  + 
Sbjct: 3   CKVARKVWKYSYLASEMQGVVREDILSVMQSLIKKMSMREVEFVAVVWWEVWHARNKLIF 62

Query: 139 VGRKIS--DLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPHWIRPPVGYVKLNVD 198
            G++++   LIAK    +A +++++ V    ++   S   VS    W  PPVG+ K+NVD
Sbjct: 63  EGKRVNPMSLIAK---AQAAVEAYQRVHGKEQTSKRSED-VSKQDQWCPPPVGFYKVNVD 122

Query: 199 ASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVLEGLYLALRLNLSLI 258
           A+V  +++ +G+GV++R+  G ++ A + S     +    EA AV  GL +AL   L+ +
Sbjct: 123 AAVHTEQQLTGLGVVIRNPQGQIIVAAIKSTKFQDNVTAAEAEAVKWGLEIALEARLAAV 182

Query: 259 IVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHVGRGCNVPAHCLARR 318
           I+E+DC E+ ++ N  TS+  E+ +W ++DI S   KF  +S  HV R CN  AH LA+R
Sbjct: 183 IIETDCIEVANLANSKTSSKKEI-MWTISDIHSYKEKFQSLSIQHVPRCCNSCAHSLAKR 242

Query: 319 GL-SIGSILWLAEFP 330
            L S+ ++ W   FP
Sbjct: 243 ALRSLETVTWKDNFP 252

BLAST of Lag0017892.1 vs. NCBI nr
Match: XP_024953934.1 (uncharacterized protein LOC112498177 [Citrus sinensis])

HSP 1 Score: 130.6 bits (327), Expect = 2.7e-26
Identity = 94/276 (34.06%), Postives = 138/276 (50.00%), Query Frame = 0

Query: 66  KSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLIMWCVG 125
           K+  E   H L+ C+ A   WK        +    +  L   L L   +  R+ + + V 
Sbjct: 17  KTGVEDVFHALMECKVATKVWKYNYGDSELQGMVREDILSLILSLLRWMPKRE-VEFVVT 76

Query: 126 CW-ALWNDRNT-VRVGRKIS--DLIAKCN-WVEAYIDSFRLVRASCRSGASSVGVVSPVP 185
            W  +W+ RN  +  GRK+S   LIAK    +EAY       + + R G      VS   
Sbjct: 77  VWKEIWHARNKFIFEGRKLSPVTLIAKAKATIEAYQRVHETEQINRRDG------VSKQK 136

Query: 186 HWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAA-MVNSFAVSYSPFNVEALA 245
            W  PP+GY K+NVDA+V    + +GI V++R+  G VVA   V +     +    EA A
Sbjct: 137 QWCPPPIGYYKVNVDAAVQAGAQLTGIRVVIRNSQGQVVATEAVKNTKPQENVSAAEAEA 196

Query: 246 VLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFS 305
           V  G+ +A+   L  +I+E+DC E+V++ N +TS+  E+ +W ++DIQS + KF  I+  
Sbjct: 197 VKRGMEMAIEAGLLAVIIETDCMEVVNLANNITSSRKEI-LWAISDIQSYMEKFQPITIQ 256

Query: 306 HVGRGCNVPAHCLARRGL-SIGSILWLAEFPAWSLS 335
           HV R CN  A  LA R L S  S+ W   FP   LS
Sbjct: 257 HVSRCCNSCAQSLAERALRSSESVTWKDNFPPDVLS 284

BLAST of Lag0017892.1 vs. ExPASy TrEMBL
Match: A0A803NGI9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 3.1e-28
Identity = 90/289 (31.14%), Postives = 145/289 (50.17%), Query Frame = 0

Query: 58   CGSCPCRLKSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDR 117
            C  C     +++ES +H L  C+ A   W+    +++++     SF D  + L +  S  
Sbjct: 847  CSLC----SNAWESVEHALFLCKHAKKVWRVAGISLNFDLVSRMSFADFLMLLSTLKSTS 906

Query: 118  DLIMWCVGCWALWNDRNTV---RVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVG 177
            ++       W LWNDRN     + G   + L AK     AY  +F+    S +S   S G
Sbjct: 907  EMEQLLCTLWFLWNDRNNFIHGKPGLSPTQLWAKS---VAYFCNFQQQSTSSKSIRDSTG 966

Query: 178  VVSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFN 237
              +  PHWI PP+  +K+NVDA+  + R + G+G+++R+  G VVAA          P  
Sbjct: 967  AAAQ-PHWISPPLDKLKMNVDAACDISRNKIGVGIIIRNSSGQVVAAYSKPLTGRLKPQE 1026

Query: 238  VEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFD 297
            +EA A+L G+  A R NLS+ + ESD   LV+ +N +++ +   G   V DI++ +    
Sbjct: 1027 MEAKALLIGINWAARCNLSINLFESDSLILVNSINSISNAISSFGDL-VLDIKNRLSYLS 1086

Query: 298  CISFSHVGRGCNVPAHCLARRGLSI-GSILWLAEFPAWSLSILKVDNAL 343
             +  SHV R  N  AH LA+  L +    +W  E P+   S++ V++AL
Sbjct: 1087 SVCVSHVKRDANQAAHGLAKHALELDDDCMWFEEIPSTIFSVV-VNDAL 1125

BLAST of Lag0017892.1 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 8.5e-26
Identity = 86/273 (31.50%), Postives = 129/273 (47.25%), Query Frame = 0

Query: 70   ESTDHPLVGCERALLFWKRVLPAVH-WEADFGQSFLDRCLFLFSTLSDRDLIMWCVGCWA 129
            ES  H    C+RA   W+ + P +    A+   SFL+    L   L  +DL +  +  W 
Sbjct: 1181 ESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWG 1240

Query: 130  LWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVPHWIRPPVG 189
            +WNDRN++  G+++S +  KC W+  ++DS    + S  S  +       V +W      
Sbjct: 1241 IWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSV 1300

Query: 190  YVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAVLEGLYLAL 249
             +KLN DA+       +  G ++RD   S+VAA         SP   E   +LEGL  A 
Sbjct: 1301 SLKLNTDAACR--GASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAA 1360

Query: 250  RLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISFSHVGRGCNVP 309
              N + + VESD    + ++     T  +   W V +IQ+L   F  ISFSH  R CN  
Sbjct: 1361 ASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNW-VMEIQALTCCFAFISFSHSSRQCNRA 1420

Query: 310  AHCLARRGLSIGS--ILWLAEFPAWSLSILKVD 340
            AH LA+ G++  S    WL  FP W L +++ D
Sbjct: 1421 AHGLAKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

BLAST of Lag0017892.1 vs. ExPASy TrEMBL
Match: A0A803PIB6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-25
Identity = 90/286 (31.47%), Postives = 137/286 (47.90%), Query Frame = 0

Query: 60   SCPCRLKS-SYESTDHPLVGCERALLFWKRVLPAVHWEADFGQ-SFL---DRCLFLFSTL 119
            S  C L S ++ES  H L  C  A   W+       ++ DF + SF+   D  LFL + L
Sbjct: 1550 SATCSLCSRAWESIGHALFSCCHAKSVWQH----TSFQLDFTKASFMKDGDYLLFLSTIL 1609

Query: 120  SDRDLIMWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVG 179
            +  +L       W +W+DRN     +++   +A  +  EAY+ +F  V+++     S V 
Sbjct: 1610 TKSELEKLFCTMWFIWSDRNNYIHCKQLKHPMAISSQAEAYLANFHSVKSATAPAVSCVA 1669

Query: 180  VVSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFN 239
              +    W+ P    +K+NVDA++   R + GIGV++RD  G V+AAM      ++    
Sbjct: 1670 ADARTVKWVPPTESNLKMNVDAALDSSRNKIGIGVIIRDSTGRVIAAMSKPVVGNFKSQE 1729

Query: 240  VEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFD 299
            +EA A+  GL  A +L L    VE+DC  LV  L G  S L       V DI   +  F 
Sbjct: 1730 MEAKAMFWGLQWAKQLQLQPHCVETDCLMLVHALQGKQSQLSSFHDL-VEDISYHLSSFS 1789

Query: 300  CISFSHVGRGCNVPAHCLARRGLSI-GSILWLAEFPAWSLSILKVD 340
                SHV R  N  AH LA++ L +    +W+ E P+   S++  D
Sbjct: 1790 NACISHVRRDANQAAHGLAKQALQLDNDCIWIEEIPSTIFSVVVND 1830

BLAST of Lag0017892.1 vs. ExPASy TrEMBL
Match: A0A1S8ACU2 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-25
Identity = 82/276 (29.71%), Postives = 134/276 (48.55%), Query Frame = 0

Query: 61  CPCRLKSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFSTLSDRDLI 120
           CP R K   E  +H ++ C+ A   WK        +    Q  L     L +  S  +L 
Sbjct: 325 CP-RCKMKKEDINHAIMVCKAAKKMWKLTPFVEEMQLLDNQDLLSMLQELVNRRSKDELR 384

Query: 121 MWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSPVP 180
           +    CW  W+ RN      K  D        EA ++S+  VR + +  A++  + +   
Sbjct: 385 LIIALCWTAWHTRNIFVFENKRQDPQISVAKAEAVVESYARVRMT-KVQAAAKAIPAKGA 444

Query: 181 HWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEALAV 240
            WI PP G+ K NVDA+V+ ++ Q G+GV++RD  G+++ A VN           EA AV
Sbjct: 445 KWIPPPQGHFKANVDAAVNKEKNQVGLGVVIRDDSGAIIIAAVNHTKYHGDVAQAEAAAV 504

Query: 241 LEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFD-CISFS 300
             GL + +  +LS +I+E+DC+++VD +    S+  E+  W +++IQ  +  F+  +   
Sbjct: 505 NFGLQVVMEASLSPLILETDCQDVVDFVLHNKSSRTEI-YWTISEIQQKLQSFNSMVELQ 564

Query: 301 HVGRGCNVPAHCLARRGL-SIGSILWLAEFPAWSLS 335
            + R CN  AH LA+  L +  S +W  E P   +S
Sbjct: 565 KIQRTCNAIAHTLAKIALVNRESCVWKGEIPCHIMS 597

BLAST of Lag0017892.1 vs. ExPASy TrEMBL
Match: A0A803QH76 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-25
Identity = 91/310 (29.35%), Postives = 139/310 (44.84%), Query Frame = 0

Query: 46   LPLTILASGGLFCGSCPCRL-KSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFL 105
            LP  +  +      S  C L K S+ES  H L  C RA L W +    V           
Sbjct: 1397 LPTAVNLAHRKIASSAACALCKCSWESVGHALFRCHRAKLVWDKFHFNVFIPNIANIKGF 1456

Query: 106  DRCLFLFSTLSDRDLIMWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRL--V 165
            D    L +  +D DL +     W +W++RN    G K       C++   Y+  +++   
Sbjct: 1457 DIYSHLAAAHNDNDLEIITCLLWCIWSERNKEIHGTKPKPAPILCSFAANYLTQYKMAAT 1516

Query: 166  RASCRSGASSVGVVSPV---------PHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRD 225
            +  C SG SS    + V         P W  P  G  KLNVDA+ +      G G ++RD
Sbjct: 1517 QQKCASGLSSTSCNASVPVDAPEKTRPQWKPPDEGCYKLNVDAACTEAGAVIGFGAILRD 1576

Query: 226  CYGSVVAAMVNSFAVSYSPFNVEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTS 285
             +G V+A +   F  ++SP  +E  A+   L  AL+L+L +  +E+D   +V+ LN  +S
Sbjct: 1577 HHGFVIAGLSKPFPGTFSPKEMEVTALFHSLQWALQLHLPISFIETDALVVVNALNKASS 1636

Query: 286  TLFEVGIWP-VTDIQSLVPKFDCISFSHVGRGCNVPAHCLARRGLSIG-SILWLAEFPAW 342
            T F    +  V D+  L+  F  +  SHV R  N  AH LA   L +     WL E P+ 
Sbjct: 1637 TKFLAPFYDLVEDVSYLLSFFPRVKVSHVKRDANEAAHALANFALRLDEDYTWLGETPSP 1696

BLAST of Lag0017892.1 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-14
Identity = 70/274 (25.55%), Postives = 122/274 (44.53%), Query Frame = 0

Query: 60  SCPCRLKSSYESTDHPLVGCERALLFWK----RVLPAVHWEADFGQSFLDRCLFL-FSTL 119
           SCP R     ES +H L  C  A + W+     ++       DF ++  +   F+  +T+
Sbjct: 199 SCP-RCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTM 258

Query: 120 SD--RDLIMWCVGCWALWNDRNTV-----RVGRKISDLIAKC---NWVEAYIDSFRLVRA 179
           SD  + L +W +  W +W  RN V     R     + L AK    +W+ A        ++
Sbjct: 259 SDFHKLLPVWLI--WRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNA-------TQS 318

Query: 180 SCRSGASSVGVVSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVN 239
             ++ + +  +      W  PP  YVK N DA   + + ++  G ++R+ YG+ ++    
Sbjct: 319 HKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSM 378

Query: 240 SFAVSYSPFNVEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVT--STLFEVGIWP 299
             A + +P   E  A+L  L        + + +E DC+ L++++NG++  S+L       
Sbjct: 379 KLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLINGISFHSSLAN----H 438

Query: 300 VTDIQSLVPKFDCISFSHVGRGCNVPAHCLARRG 317
           + DI     KF  I F  + R  N  AH LA+ G
Sbjct: 439 LEDISFWANKFASIQFGFIRRKGNKLAHVLAKYG 458

BLAST of Lag0017892.1 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 57.0 bits (136), Expect = 3.5e-08
Identity = 53/199 (26.63%), Postives = 75/199 (37.69%), Query Frame = 0

Query: 119 LIMWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGVVSP 178
           L +W +  W LWN RN +   R+           +  +  +          +    + S 
Sbjct: 442 LPLWIL--WRLWNSRNILYFQRRHVPWETTLQLAQMDLQEWNEAVTPSHINSHGTTIRSH 501

Query: 179 VPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFNVEAL 238
           +P+W RP  G+VK NVD S          G +VRD  G    A         +    E  
Sbjct: 502 IPNWRRPTNGWVKCNVDGSFINGNIPGKGGWIVRDHNGRYEFAGQAIGNCIDNALECEFQ 561

Query: 239 AVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFDCISF 298
           A+L  +          I  E D KE+ D++NG+    F    W + DI     KF+   F
Sbjct: 562 ALLMAMQHCWSKGYRRICFEGDNKEMFDLINGL-KMHFGAFNW-IRDILWWASKFEQTQF 621

Query: 299 SHVGRGCNVPAHCLARRGL 318
               R  N PA  LAR  L
Sbjct: 622 QWTNRQNNKPADILARTPL 636

BLAST of Lag0017892.1 vs. TAIR 10
Match: AT2G04420.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-07
Identity = 49/203 (24.14%), Postives = 80/203 (39.41%), Query Frame = 0

Query: 127 WALWNDRNTV----------RVGRKISDLIAKCNWVEAYIDSFR--LVRASCRSGASSVG 186
           W LW  RN +             ++  D + +    + YI S     VR  C +  S+  
Sbjct: 2   WRLWRSRNQLVFQHQNLSWQSTLKRAKDDVQEWENAQEYIQSLNHYTVREGCNNREST-- 61

Query: 187 VVSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSYSPFN 246
                  W +PP+G++K N D S +   +Q+  G L+RD  G    A         +   
Sbjct: 62  ----HQKWEQPPMGWIKCNYDGSFNYRTQQTNSGWLIRDDKGFYKGAAQAVGGTMNNALE 121

Query: 247 VEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVPKFD 306
            E  A++  +          +I E D K++ ++LN      F    W + +  S   +F+
Sbjct: 122 SELQALVMAMQHTWSQGYRKVIFEGDSKQVEELLNR-KQMHFGAFNW-IREAWSWSKRFE 181

Query: 307 CISFSHVGRGCNVPAHCLARRGL 318
            + FS   R  N PA  LA+  L
Sbjct: 182 EVIFSWTPRTNNQPADMLAKSHL 196

BLAST of Lag0017892.1 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-06
Identity = 67/274 (24.45%), Postives = 104/274 (37.96%), Query Frame = 0

Query: 63  CRLKSSYESTDHPLVGCERALLFWKRVLPAVHWEADFGQSFLDRCLFLFST-------LS 122
           C    + E++ H L  C+ A   W  + P        G   L+    L  T       + 
Sbjct: 33  CARCGAPETSTHMLFHCDFAAQVW-NLAPLQLGHIPIGTPILEALNLLKKTIVLPPVGIH 92

Query: 123 DRDLIMWCVGCWALWNDRNTVRVGRKISDLIAKCNWVEAYIDSFRLVRASCRSGASSVGV 182
              L  W   CW +W  RN +        +I      +A  D+  L   S +     V V
Sbjct: 93  TATLFPWI--CWHIWKARNQLIFQNSHFSVIETV--TKAVQDA--LAWQSAQLALPKVRV 152

Query: 183 VSPVPHWIRPPVGYVKLNVDASVSMDRRQSGIGVLVRDCYGSVVAAMVNSFAVSY----S 242
            +P P+            VDA+       +G G + +    S     + +F+       S
Sbjct: 153 ATPRPNSTTTLTHDFLCYVDAAWQQQSSLAGSGWVFQATSHS--EKEITTFSAGCRRFPS 212

Query: 243 PFNVEALAVLEGLYLALRLNLSLIIVESDCKELVDMLNGVTSTLFEVGIWPVTDIQSLVP 302
           P   EA A+   +  AL+L  S ++V SD K +VD LN   S     G+  + +I+S+  
Sbjct: 213 PLAAEAWAIKSAMLHALQLERSDLLVLSDSKSIVDALNSNVSLNEIFGL--LVEIRSIRN 272

Query: 303 KFDCISFSHVGRGCNVPAHCLARRGLSIGSILWL 326
           +F  ISF  + R  N  A   A+  L I   + L
Sbjct: 273 RFRSISFQFIPRLVNSIADAAAKLSLCISGNIGL 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_024046732.11.3e-2832.46uncharacterized protein LOC112101057 [Citrus clementina][more]
XP_024950112.11.9e-2731.65uncharacterized protein LOC112496847 [Citrus sinensis][more]
XP_024033146.14.2e-2732.55uncharacterized protein LOC112095446 [Citrus clementina][more]
XP_015385025.19.3e-2732.16uncharacterized protein LOC107176703 [Citrus sinensis][more]
XP_024953934.12.7e-2634.06uncharacterized protein LOC112498177 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803NGI93.1e-2831.14Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DX308.5e-2631.50uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803PIB61.4e-2531.47Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A1S8ACU21.9e-2529.71Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1[more]
A0A803QH762.5e-2529.35Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09510.11.5e-1425.55Ribonuclease H-like superfamily protein [more]
AT2G33160.13.5e-0826.63glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro... [more]
AT2G04420.15.1e-0724.14Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G10000.11.9e-0624.45Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 188..318
e-value: 6.3E-14
score: 54.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 193..315
e-value: 1.9E-26
score: 92.3
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 179..334
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 179..334
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 192..314
e-value: 1.7357E-23
score: 91.992
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 188..314

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0017892Lag0017892gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0017892.1.exon2Lag0017892.1.exon2exon
Lag0017892.1.exon1Lag0017892.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0017892.1cds.Lag0017892.1_2CDS
cds.Lag0017892.1cds.Lag0017892.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0017892.1Lag0017892.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity