Lag0017579.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017579.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Calcium-binding EF-hand family protein
Location	chr5: 5580138 .. 5580581 (+)
Sequence length	444
RNA-Seq Expression	Lag0017579.1
Synteny	Lag0017579.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGATCTGAGCGATGACCAGAAATCCTCCATGAAAGAGGCCTTCACCCTCTTCGATACCGATGGCGATGGCCGGATCGCCCCCTCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATCATCGCCGAGGAGAATCTCACTTCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGATAAGGACAACACCGGCTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGCGAGAAGCTGGAGCCCTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTAGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

mRNA sequence

ATGGGCAAAGATCTGAGCGATGACCAGAAATCCTCCATGAAAGAGGCCTTCACCCTCTTCGATACCGATGGCGATGGCCGGATCGCCCCCTCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATCATCGCCGAGGAGAATCTCACTTCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGATAAGGACAACACCGGCTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGCGAGAAGCTGGAGCCCTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTAGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGATGACCAGAAATCCTCCATGAAAGAGGCCTTCACCCTCTTCGATACCGATGGCGATGGCCGGATCGCCCCCTCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATCATCGCCGAGGAGAATCTCACTTCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGATAAGGACAACACCGGCTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGCGAGAAGCTGGAGCCCTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTAGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Protein sequence

MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGSDGRIRYEDFIARMVAK

Homology

[Close]

BLAST of Lag0017579.1 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 292.0 bits (746), Expect = 2.9e-75
Identity = 145/147 (98.64%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Lag0017579.1 vs. NCBI nr
Match: XP_022152721.1 (probable calcium-binding protein CML13 [Momordica charantia])

HSP 1 Score: 287.7 bits (735), Expect = 5.5e-74
Identity = 142/147 (96.60%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60
           MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREV+VGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Lag0017579.1 vs. NCBI nr
Match: XP_004147942.1 (probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetical protein Csa_011609 [Cucumis sativus])

HSP 1 Score: 287.3 bits (734), Expect = 7.2e-74
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Lag0017579.1 vs. NCBI nr
Match: XP_008448907.1 (PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML13 [Cucumis melo subsp. melo] >TYK19478.1 putative calcium-binding protein CML13 [Cucumis melo var. makuwa])

HSP 1 Score: 285.8 bits (730), Expect = 2.1e-73
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Lag0017579.1 vs. NCBI nr
Match: XP_023005593.1 (probable calcium-binding protein CML13 [Cucurbita maxima])

HSP 1 Score: 285.0 bits (728), Expect = 3.6e-73
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60
           MGKDLS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPE FDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.1e-67
Identity = 126/148 (85.14%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSDDQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  SPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 3.7e-65
Identity = 121/148 (81.76%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS+DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  SPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.2e-59
Identity = 108/146 (73.97%), Postives = 130/146 (89.04%), Query Frame = 0


Query: 2   GKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTSP 61
           GK+LS++Q +SM+EAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LT+P
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++PEPFDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGSDGRIRYEDFIARMVAK 148
           EWIREV+V  DG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

[Close]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot
Match: Q03509 (Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 5.3e-35
Identity = 73/149 (48.99%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot
Match: P62200 (Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 6.9e-35
Identity = 73/149 (48.99%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of Lag0017579.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876882.1	2.9e-75	98.64	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_022152721.1	5.5e-74	96.60	probable calcium-binding protein CML13 [Momordica charantia]	[more]
XP_004147942.1	7.2e-74	97.28	probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetica...	[more]
XP_008448907.1	2.1e-73	96.60	PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML...	[more]
XP_023005593.1	3.6e-73	96.60	probable calcium-binding protein CML13 [Cucurbita maxima]	[more]

BLAST of Lag0017579.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	1.1e-67	85.14	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	3.7e-65	81.76	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	5.2e-59	73.97	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q03509	5.3e-35	48.99	Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2	[more]
P62200	6.9e-35	48.99	Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 12..40 e-value: 8.4E-5 score: 31.9 coord: 83..111 e-value: 4.7E-4 score: 29.5 coord: 119..147 e-value: 2.3 score: 14.8
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 3.6E-6 score: 26.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 1.7E-13 score: 50.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 13.062845
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 12.281773
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..55 e-value: 1.14154E-8 score: 46.7721
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 9.6E-21 score: 75.8
None	No IPR available	GENE3D	1.10.238.10		coord: 81..145 e-value: 1.2E-18 score: 69.0
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0017579	Lag0017579	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0017579.1.exon1	Lag0017579.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0017579.1	cds.Lag0017579.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0017579.1	Lag0017579.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding