Lag0017328.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017328.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ERD (Early-responsive to dehydration stress)family protein
Location	chr5: 2230966 .. 2231680 (+)
Sequence length	423
RNA-Seq Expression	Lag0017328.1
Synteny	Lag0017328.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGTTTGCTACGTACTTTATAATGACTATAGCAAAGTAGCACAAATGCGGTTAAATTTCCTGAGTTCAGAAAATAGACATGTTAAGCATTACACGGTAAGTAAGAATATTATTCATCTTTTCAAGTCTTCTTAACATTATCATTAATCATTAATAATAATGTTAATTTGCTTTGTAGGTATTGGTTAGGAATGTACAATCTAATTCTGGTACCTCCACATCTAATGTCATCGATAACTTCTTACAAATAAGTTATCTGCAACACTATCTATTTTATCAGGTGACGATAATTATATAAATACTGCAAATAATAAATAAACTATATATATAATCTTTAATTTTTTTTATTAATTCATTCTTTTATTTTTTAGGGGTATATAAATCTGATGAATTTGATAAATTAGCACATGCGAGAACAATTTTGAAAGAAAATTGGAGTTGCAATTCTTTCTTTTCATCCTCTGAAAACTTAATTAAATTGGTTATACAGGATGTTCCTGAAATTGGTTATGTAGGGTTTTTTTCATGGGTTGGTGCTGCAATTCTTTCTTTTCATCCTCCGAAAACTTTTGATCTTCTTGTCGAAAGTGGAACGATATACATCACTTTCGGAGATAGAATACTAGGACACTGCGAAATACTATTATTTCATGGTGGTCAATGTTATCTTAGGCAGTATTGTGGCTGGCATGACATTGGAGGAACTGCATAG

mRNA sequence

ATGTGGGTTTGCTACGTACTTTATAATGACTATAGCAAAGTAGCACAAATGCGGTTAAATTTCCTGAGTTCAGAAAATAGACATGTTAAGCATTACACGGTATTGGTTAGGAATGTACAATCTAATTCTGGTACCTCCACATCTAATGTCATCGATAACTTCTTACAAATAAGTTATCTGCAACACTATCTATTTTATCAGGATGTTCCTGAAATTGGTTATGTAGGGTTTTTTTCATGGGTTGGTGCTGCAATTCTTTCTTTTCATCCTCCGAAAACTTTTGATCTTCTTGTCGAAAGTGGAACGATATACATCACTTTCGGAGATAGAATACTAGGACACTGCGAAATACTATTATTTCATGGTGGTCAATGTTATCTTAGGCAGTATTGTGGCTGGCATGACATTGGAGGAACTGCATAG

Coding sequence (CDS)

ATGTGGGTTTGCTACGTACTTTATAATGACTATAGCAAAGTAGCACAAATGCGGTTAAATTTCCTGAGTTCAGAAAATAGACATGTTAAGCATTACACGGTATTGGTTAGGAATGTACAATCTAATTCTGGTACCTCCACATCTAATGTCATCGATAACTTCTTACAAATAAGTTATCTGCAACACTATCTATTTTATCAGGATGTTCCTGAAATTGGTTATGTAGGGTTTTTTTCATGGGTTGGTGCTGCAATTCTTTCTTTTCATCCTCCGAAAACTTTTGATCTTCTTGTCGAAAGTGGAACGATATACATCACTTTCGGAGATAGAATACTAGGACACTGCGAAATACTATTATTTCATGGTGGTCAATGTTATCTTAGGCAGTATTGTGGCTGGCATGACATTGGAGGAACTGCATAG

Protein sequence

MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQHYLFYQDVPEIGYVGFFSWVGAAILSFHPPKTFDLLVESGTIYITFGDRILGHCEILLFHGGQCYLRQYCGWHDIGGTA

Homology

[Close]

BLAST of Lag0017328.1 vs. NCBI nr
Match: XP_038893055.1 (CSC1-like protein At1g32090 [Benincasa hispida])

HSP 1 Score: 82.4 bits (202), Expect = 3.4e-12
Identity = 37/69 (53.62%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 1   MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYL 60
           +W+CY+LY +Y  VAQMRLNFL+S+ R  + +TVLVRNV  +SG STS+V+D F   ++ 
Sbjct: 169 LWICYMLYKEYDNVAQMRLNFLASQRRRAEQFTVLVRNVPHSSGRSTSDVVDQFFHKNHP 228

Query: 61  QHYLFYQDV 70
           +HYL +Q V
Sbjct: 229 EHYLSHQAV 237

[Close]

BLAST of Lag0017328.1 vs. NCBI nr
Match: XP_004146108.1 (CSC1-like protein At1g32090 [Cucumis sativus] >KGN55705.1 hypothetical protein Csa_011603 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-11
Identity = 36/69 (52.17%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 1   MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYL 60
           +W+CY+LY +Y+ VAQMRLNFL+S+ R  + +TVLVRNV  +SG STS+ +D F   ++ 
Sbjct: 169 IWICYLLYKEYNNVAQMRLNFLASQRRRAEQFTVLVRNVPHSSGRSTSDSVDQFFHKNHP 228

Query: 61  QHYLFYQDV 70
           +HYL +Q V
Sbjct: 229 EHYLSHQAV 237

[Close]

BLAST of Lag0017328.1 vs. NCBI nr
Match: XP_008448614.1 (PREDICTED: CSC1-like protein At1g32090 [Cucumis melo] >XP_016900574.1 PREDICTED: CSC1-like protein At1g32090 [Cucumis melo] >KAA0053004.1 CSC1-like protein [Cucumis melo var. makuwa] >TYK11460.1 CSC1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-11
Identity = 36/69 (52.17%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 1   MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYL 60
           +W+CY+LY +Y  VAQMRLNFL+S+ R  + +TVLVRNV  +SG STS+ +D F   ++ 
Sbjct: 169 IWICYLLYKEYDNVAQMRLNFLASQRRRAEQFTVLVRNVPHSSGRSTSDSVDQFFHKNHP 228

Query: 61  QHYLFYQDV 70
           +HYL +Q V
Sbjct: 229 EHYLSHQAV 237

[Close]

BLAST of Lag0017328.1 vs. NCBI nr
Match: XP_023005789.1 (CSC1-like protein At1g32090 [Cucurbita maxima])

HSP 1 Score: 79.0 bits (193), Expect = 3.7e-11
Identity = 41/102 (40.20%), Postives = 60/102 (58.82%), Query Frame = 0


Query: 1   MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYL 60
           +W+C++LY +Y  VAQMR+NFL+S+ R  + +TVLVRNV   SG STS+ ID F   ++ 
Sbjct: 169 LWICFMLYKEYDNVAQMRMNFLASQRRRAEQFTVLVRNVPHVSGRSTSDTIDQFFHKNHP 228

Query: 61  QHYLFYQDVPEIGYVG--------FFSWVGAAILSF--HPPK 93
           +HYL +Q +               F +W+   +L F  HP K
Sbjct: 229 EHYLSHQAIYNANKFAKLAKKRARFQNWLDYNLLKFERHPDK 270

[Close]

BLAST of Lag0017328.1 vs. NCBI nr
Match: KAA8525709.1 (hypothetical protein F0562_007564 [Nyssa sinensis])

HSP 1 Score: 78.2 bits (191), Expect = 6.4e-11
Identity = 35/68 (51.47%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 2   WVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQ 61
           W+CY+LY +Y +VA MRLNF++S+ R  + +TVLVRNV   SG S S+ +DNF Q ++  
Sbjct: 170 WICYLLYKEYDRVASMRLNFMASQRRRAEQFTVLVRNVPHVSGRSISDSVDNFFQTNHPD 229

Query: 62  HYLFYQDV 70
           HYL +Q V
Sbjct: 230 HYLCHQAV 237

[Close]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot
Match: F4HYR3 (CSC1-like protein At1g62320 OS=Arabidopsis thaliana OX=3702 GN=At1g62320 PE=3 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 2.5e-10
Identity = 32/68 (47.06%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 2   WVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQ 61
           W CYVL  +Y K+A MRL+FL SE R    +TVLVRNV  +S  S S  + +F  +++  
Sbjct: 171 WTCYVLMKEYEKIAAMRLSFLQSEKRRADQFTVLVRNVPPDSDESISENVQHFFLVNHPD 230

Query: 62  HYLFYQDV 70
           HYL +Q V
Sbjct: 231 HYLTHQVV 238

[Close]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot
Match: Q9LVE4 (CSC1-like protein At3g21620 OS=Arabidopsis thaliana OX=3702 GN=At3g21620 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-09
Identity = 28/68 (41.18%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 2   WVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQ 61
           W C+VL  +Y  +A MRL FL+SE+R    +TVLVRN+  +   S S ++++F ++++  
Sbjct: 169 WTCFVLQREYKHIASMRLQFLASEHRRPDQFTVLVRNIPPDPDESVSELVEHFFKVNHPD 228

Query: 62  HYLFYQDV 70
           +YL YQ V
Sbjct: 229 YYLTYQAV 236

[Close]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot
Match: B5TYT3 (CSC1-like protein At1g11960 OS=Arabidopsis thaliana OX=3702 GN=At1g11960 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-09
Identity = 31/68 (45.59%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 2   WVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQ 61
           W CYVL  +Y KVA MRL FL +E R    +TVLVRNV ++   S S+ +++F  +++  
Sbjct: 171 WTCYVLMKEYEKVAAMRLAFLQNEQRRPDQFTVLVRNVPADPDESISDSVEHFFLVNHPD 230

Query: 62  HYLFYQDV 70
           HYL +Q V
Sbjct: 231 HYLTHQVV 238

[Close]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot
Match: Q9FVQ5 (CSC1-like protein At1g32090 OS=Arabidopsis thaliana OX=3702 GN=At1g32090 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-09
Identity = 28/68 (41.18%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 2   WVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYLQ 61
           W C++LY +Y+ VA MRL +L+S+ R  + +TV+VRNV    G S  + +D F + ++ +
Sbjct: 171 WACFMLYREYNNVAIMRLQYLASQRRRPEQFTVVVRNVPDMPGHSVPDTVDQFFKTNHPE 230

Query: 62  HYLFYQDV 70
           HYL +Q V
Sbjct: 231 HYLCHQAV 238

[Close]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot
Match: Q5XEZ5 (Calcium permeable stress-gated cation channel 1 OS=Arabidopsis thaliana OX=3702 GN=CSC1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-09
Identity = 29/69 (42.03%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 1   MWVCYVLYNDYSKVAQMRLNFLSSENRHVKHYTVLVRNVQSNSGTSTSNVIDNFLQISYL 60
           +W CYVL  +Y  +A MRL F++SE R    +TVLVRNV  ++  S S ++++F  +++ 
Sbjct: 171 IWTCYVLMKEYETIANMRLQFVASEARRPDQFTVLVRNVPPDADESVSELVEHFFLVNHP 230

Query: 61  QHYLFYQDV 70
            HYL +Q V
Sbjct: 231 DHYLTHQVV 239

The following BLAST results are available for this feature:

BLAST of Lag0017328.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893055.1	3.4e-12	53.62	CSC1-like protein At1g32090 [Benincasa hispida]	[more]
XP_004146108.1	1.7e-11	52.17	CSC1-like protein At1g32090 [Cucumis sativus] >KGN55705.1 hypothetical protein C...	[more]
XP_008448614.1	2.2e-11	52.17	PREDICTED: CSC1-like protein At1g32090 [Cucumis melo] >XP_016900574.1 PREDICTED:...	[more]
XP_023005789.1	3.7e-11	40.20	CSC1-like protein At1g32090 [Cucurbita maxima]	[more]
KAA8525709.1	6.4e-11	51.47	hypothetical protein F0562_007564 [Nyssa sinensis]	[more]

BLAST of Lag0017328.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HYR3	2.5e-10	47.06	CSC1-like protein At1g62320 OS=Arabidopsis thaliana OX=3702 GN=At1g62320 PE=3 SV...	[more]
Q9LVE4	1.2e-09	41.18	CSC1-like protein At3g21620 OS=Arabidopsis thaliana OX=3702 GN=At3g21620 PE=2 SV...	[more]
B5TYT3	1.2e-09	45.59	CSC1-like protein At1g11960 OS=Arabidopsis thaliana OX=3702 GN=At1g11960 PE=2 SV...	[more]
Q9FVQ5	1.6e-09	41.18	CSC1-like protein At1g32090 OS=Arabidopsis thaliana OX=3702 GN=At1g32090 PE=1 SV...	[more]
Q5XEZ5	2.1e-09	42.03	Calcium permeable stress-gated cation channel 1 OS=Arabidopsis thaliana OX=3702 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR13018:SF98	TO DEHYDRATION PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..69
IPR045122	Calcium permeable stress-gated cation channel 1-like	PANTHER	PTHR13018	PROBABLE MEMBRANE PROTEIN DUF221-RELATED	coord: 1..69

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0017328	Lag0017328	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0017328.1.exon1	Lag0017328.1.exon1	exon
Lag0017328.1.exon2	Lag0017328.1.exon2	exon
Lag0017328.1.exon3	Lag0017328.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0017328.1	cds.Lag0017328.1	CDS
cds.Lag0017328.1	cds.Lag0017328.1_2	CDS
cds.Lag0017328.1	cds.Lag0017328.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0017328.1	Lag0017328.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034220	ion transmembrane transport
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005227	calcium activated cation channel activity