Lag0011927.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011927.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Calcium-binding EF-hand family protein
Location	chr1: 35026704 .. 35027300 (-)
Sequence length	597
RNA-Seq Expression	Lag0011927.1
Synteny	Lag0011927.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCACTCCGCCGCCGCCGATGTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTACGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCCACTCCGATCACAAGGACGACAACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTTCCGGATCGCCTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTACTGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGGATCACCGCGGAGGAGCTTCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTTGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

mRNA sequence

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCACTCCGCCGCCGCCGATGTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTACGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCCACTCCGATCACAAGGACGACAACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTTCCGGATCGCCTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTACTGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGGATCACCGCGGAGGAGCTTCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTTGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCCAAGCGCCTCTTCCGATCCAAGAAGAGCCACTCCGCCGCCGCCGATGTTTCTCGCTGCGATCCGCCCTCCTTCGGCTCCGGTACCTCCTCCTCCTCCTCCTCTTCCGAGAACTCCTTCAAGTCTCACCCCGCCGCCGGCTACGGAACTCCAACCAGCGTCCTCCCCGCGGACTGGTCCGATTCCCACTCCGATCACAAGGACGACAACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTTCCGGATCGCCTCCGAGGAGGAGGTCGCCATGATGCTGAGCGAAGTCGACGGCGGCGGATTCATTCGCCTCGAGGCCTTGATGGCTCGGGCCGGGCCGGCGTTCGAACCGGCTGGAGAGTCGGAGCTACTGGAGACGTTTGAGTTTTTCGACGCCGATCACGACGGGAGGATCACCGCGGAGGAGCTTCACGGCGTTTTCAGTGCGATCGGCGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTTGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGGTGA

Protein sequence

MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPTSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAEFVRMMELQR

Homology

[Close]

BLAST of Lag0011927.1 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 296.2 bits (757), Expect = 2.1e-76
Identity = 161/198 (81.31%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPT 60
           MKIPKRFSPKRLFRSKK+ S  A+VSR DPPSFGSGTSSSSSSSEN FK H  AGYGTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--AEVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++++GVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESEL ETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 199
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lag0011927.1 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.4 bits (755), Expect = 3.6e-76
Identity = 161/198 (81.31%), Postives = 172/198 (86.87%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPT 60
           MKIPKRFSPKRLFRSKK+ S  ADVSR DPPSFGSGTSSSSSSSEN FK H  AGYGTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++++GVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+   ESEL ETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 199
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lag0011927.1 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 288.5 bits (737), Expect = 4.4e-74
Identity = 160/198 (80.81%), Postives = 171/198 (86.36%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPT 60
           MKIPKRFSPKRLFRSKK+ S  ADVSR DPPSFGSGTSSSSSSSEN FK H  AGYGTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++++ VVSRKELEAIL RIASEEEVAMMLSEVD GGFIRL+ LMAR
Sbjct: 61  SVL-AGCSD--SDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESEL ETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 199
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Lag0011927.1 vs. NCBI nr
Match: XP_038883626.1 (probable calcium-binding protein CML35 [Benincasa hispida])

HSP 1 Score: 279.6 bits (714), Expect = 2.0e-71
Identity = 158/203 (77.83%), Postives = 166/203 (81.77%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSH-PAAGYGTP 60
           MKIPKRFSPKRLFRSKK HS  ADVSR DPPSFGSGTSSSSSSSE  FK +  A GYGTP
Sbjct: 1   MKIPKRFSPKRLFRSKKYHS--ADVSRSDPPSFGSGTSSSSSSSETFFKPNATATGYGTP 60

Query: 61  TSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGG-----GFIRL 120
           TSVLP DW D  S  ++++GVVSRKELEAIL RIASEEEVAMMLSEV  G     GFI L
Sbjct: 61  TSVLPPDWPD--SGQRENDGVVSRKELEAILSRIASEEEVAMMLSEVSSGGGGGDGFIWL 120

Query: 121 EALMARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMI 180
           E LMA+ G   E AGESEL ETFEFFDAD DGRITAEELH VF +IGDE+CTLEDCRRMI
Sbjct: 121 EELMAQVGTGVEAAGESELRETFEFFDADQDGRITAEELHEVFRSIGDEQCTLEDCRRMI 180

Query: 181 ADVDENGDGFVCFAEFVRMMELQ 198
            DVDENGDG VCFAEFVRMMELQ
Sbjct: 181 RDVDENGDGVVCFAEFVRMMELQ 199

[Close]

BLAST of Lag0011927.1 vs. NCBI nr
Match: XP_022153870.1 (probable calcium-binding protein CML36 [Momordica charantia])

HSP 1 Score: 279.6 bits (714), Expect = 2.0e-71
Identity = 160/205 (78.05%), Postives = 167/205 (81.46%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVS-RCDPPSFGSGTSSSSSSSENSFKSHPAA---GY 60
           MKIPKRFSPKRLFRSKK+    ADVS R DPPSFGS TSSSSSSSEN FK   AA   GY
Sbjct: 1   MKIPKRFSPKRLFRSKKNR---ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGY 60

Query: 61  GTPTSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEVDGGGFI 120
           GTPTSVLP    DS +D  ++NGVVSRKELEAIL RI     SEEEVAMML E DG GFI
Sbjct: 61  GTPTSVLP----DSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFI 120

Query: 121 RLEALMARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRR 180
           RLEALM R G  F PA E+EL E FEFFDADHDGRITAEELHGVFS+IGDERCTLEDCRR
Sbjct: 121 RLEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRR 180

Query: 181 MIADVDENGDGFVCFAEFVRMMELQ 198
           MIADVDENGDGFVCF+EFVRMMELQ
Sbjct: 181 MIADVDENGDGFVCFSEFVRMMELQ 198

[Close]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 109/206 (52.91%), Postives = 136/206 (66.02%), Query Frame = 0


Query: 6   RFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSS-SSENSFKSHPAAGYGTPTSVLP 65
           R SPKRLFR+K    + A VSR +P SF S  SSSSS  S  + K  P A   TP SVLP
Sbjct: 9   RLSPKRLFRTK----SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLP 68

Query: 66  ADWSDSHSD--------HKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEVDG--GG 125
            +  D +++         +DD+GVVSR +L A++ R++    S+EEV++ML EVDG  GG
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMAR-AGPAFEPAGES-ELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLE 185
            I LE L +R AG + E + E+ EL E FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFAEFVRMM 195
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.4e-37
Identity = 109/212 (51.42%), Postives = 135/212 (63.68%), Query Frame = 0


Query: 3   IPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPTSV 62
           IPKRF  +   RS  S S  A        SFGS +SSS    +NS       G  TPTS+
Sbjct: 7   IPKRFFIRSKDRSTVSKSPTAF-------SFGSASSSSGQDCKNS-GGDGGGGSVTPTSI 66

Query: 63  LPADWSD-SHSD--------HKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEV--D 122
           LP   S  S+ +         +D++G VSR +LE++L R+     +EEE+ +ML EV  D
Sbjct: 67  LPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCD 126

Query: 123 GGGFIRLEALMARAGPAFEPAGES-ELLETFEFFDADHDGRITAEELHGVFSAIGDERCT 182
           G G IRLE L +R   + +PA +S EL ETFEFFDAD DG I+A+EL  VFS IGDERCT
Sbjct: 127 GDGTIRLEELASRV-VSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 186

Query: 183 LEDCRRMIADVDENGDGFVCFAEFVRMMELQR 199
           L+DC+RMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 187 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 51/128 (39.84%), Postives = 80/128 (62.50%), Query Frame = 0


Query: 75  KDDNGVVSRKELEAILFRI---ASEEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG 134
           K+++G +S  EL+ ++  +   AS+EE   M+ E  +DG GFI L+  +A    + + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 135 ES---ELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVC 194
            S   +L E F+ +D D +GRI+A ELH V   +G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 51/130 (39.23%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 75  KDDNGVVSRKELEAILFRI---ASEEEVAMMLS--EVDGGGFIRLEALMARAGPAFEPAG 134
           K+ +G +S  EL+ ++  +   AS EE   M+   ++DG GFI L+  +A         G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 135 E-----SELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGF 194
                 S+L E FE +D D +GRI+A+ELH V   +G E+C+++DC++MI+ VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

[Close]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.2e-15
Identity = 64/164 (39.02%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 40  SSSSSENSFKSHPAAGYGTPTSVLPADWSDSHSDHKDDN--GVVSRKELEAI---LFRIA 99
           S S S    ++ PA+G G+P    P +  +      D N  G +SR EL A+   L   A
Sbjct: 14  SGSKSPPLPQADPASGGGSPAPT-PEEEMERVFRKFDANGDGRISRSELGALFESLGHAA 73

Query: 100 SEEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG--ESELLETFEFFDADHDGRITA 159
           +++E+A M++E   DG GFI L+   A    A   A   E +L   F  FDAD +G I+A
Sbjct: 74  TDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISA 133

Query: 160 EELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAEFVRMM 195
            EL  V   +G E+ T++ CRRMI  VD+NGDG + F EF  MM
Sbjct: 134 AELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 1.0e-76
Identity = 161/198 (81.31%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPT 60
           MKIPKRFSPKRLFRSKK+ S  A+VSR DPPSFGSGTSSSSSSSEN FK H  AGYGTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--AEVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++++GVVSRKELEAIL RIASEEEVAMMLSEVDGGGFIRL+ LMAR
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESEL ETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 199
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.1e-74
Identity = 160/198 (80.81%), Postives = 171/198 (86.36%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPT 60
           MKIPKRFSPKRLFRSKK+ S  ADVSR DPPSFGSGTSSSSSSSEN FK H  AGYGTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPS--ADVSRSDPPSFGSGTSSSSSSSENFFKPH-TAGYGTPT 60

Query: 61  SVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGGGFIRLEALMAR 120
           SVL A  SD  SD ++++ VVSRKELEAIL RIASEEEVAMMLSEVD GGFIRL+ LMAR
Sbjct: 61  SVL-AGCSD--SDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMAR 120

Query: 121 AGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDEN 180
            G  F+  GESEL ETFEFFDADHDGRI+AEELHGV+ +IGDERCTLEDCRRMI DVDE+
Sbjct: 121 VGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 180

Query: 181 GDGFVCFAEFVRMMELQR 199
           GDGFVCF EFVRMMELQR
Sbjct: 181 GDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL
Match: A0A6J1DI26 (probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC111021283 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 9.8e-72
Identity = 160/205 (78.05%), Postives = 167/205 (81.46%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVS-RCDPPSFGSGTSSSSSSSENSFKSHPAA---GY 60
           MKIPKRFSPKRLFRSKK+    ADVS R DPPSFGS TSSSSSSSEN FK   AA   GY
Sbjct: 1   MKIPKRFSPKRLFRSKKNR---ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGY 60

Query: 61  GTPTSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEVDGGGFI 120
           GTPTSVLP    DS +D  ++NGVVSRKELEAIL RI     SEEEVAMML E DG GFI
Sbjct: 61  GTPTSVLP----DSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEFDGDGFI 120

Query: 121 RLEALMARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRR 180
           RLEALM R G  F PA E+EL E FEFFDADHDGRITAEELHGVFS+IGDERCTLEDCRR
Sbjct: 121 RLEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRR 180

Query: 181 MIADVDENGDGFVCFAEFVRMMELQ 198
           MIADVDENGDGFVCF+EFVRMMELQ
Sbjct: 181 MIADVDENGDGFVCFSEFVRMMELQ 198

[Close]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.4e-71
Identity = 159/201 (79.10%), Postives = 165/201 (82.09%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPA-AGYGTP 60
           MKIPKRFSPKRLFRSKK HS  ADVSR DPPSFGSGTSSSSSSSEN FK++    GYGTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHHS--ADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTP 61

Query: 61  TSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL        SD + + GVVSRKELEAIL RIASEEEVAMML EVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESE+ ETFEFFDAD DGRITAEELHGVF +IGDERCTLEDC RMI DV
Sbjct: 122 MARVGSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 199
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 8.3e-71
Identity = 159/201 (79.10%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPA-AGYGTP 60
           MKIPKRFSPKRLFRSKK  S  ADVSR DPPSFGSGTSSSSSSSEN FK +    GYGTP
Sbjct: 2   MKIPKRFSPKRLFRSKKHQS--ADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTP 61

Query: 61  TSVLPADWSDSHSDHKDDNGVVSRKELEAILFRIASEEEVAMMLSEVDGG--GFIRLEAL 120
           TSVL        SD +++ GVV+RKELEAIL RIASEEEVAMMLSEVD G  GFIRLE L
Sbjct: 62  TSVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEEL 121

Query: 121 MARAGPAFEPAGESELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADV 180
           MAR G   E AGESEL ETFEFFDAD DGRITAEELHGVF +IGDE+CTLEDCRRMI DV
Sbjct: 122 MARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDV 181

Query: 181 DENGDGFVCFAEFVRMMELQR 199
           DENGDGFVCFAEFVRMMELQR
Sbjct: 182 DENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lag0011927.1 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 164.5 bits (415), Expect = 8.9e-41
Identity = 109/206 (52.91%), Postives = 136/206 (66.02%), Query Frame = 0


Query: 6   RFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSS-SSENSFKSHPAAGYGTPTSVLP 65
           R SPKRLFR+K    + A VSR +P SF S  SSSSS  S  + K  P A   TP SVLP
Sbjct: 9   RLSPKRLFRTK----SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLP 68

Query: 66  ADWSDSHSD--------HKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEVDG--GG 125
            +  D +++         +DD+GVVSR +L A++ R++    S+EEV++ML EVDG  GG
Sbjct: 69  QNSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGG 128

Query: 126 FIRLEALMAR-AGPAFEPAGES-ELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLE 185
            I LE L +R AG + E + E+ EL E FE FD D +G+I+AEELH VF  IGDERCTLE
Sbjct: 129 CISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLE 188

Query: 186 DCRRMIADVDENGDGFVCFAEFVRMM 195
           +C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 ECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lag0011927.1 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.4e-38
Identity = 109/212 (51.42%), Postives = 135/212 (63.68%), Query Frame = 0


Query: 3   IPKRFSPKRLFRSKKSHSAAADVSRCDPPSFGSGTSSSSSSSENSFKSHPAAGYGTPTSV 62
           IPKRF  +   RS  S S  A        SFGS +SSS    +NS       G  TPTS+
Sbjct: 7   IPKRFFIRSKDRSTVSKSPTAF-------SFGSASSSSGQDCKNS-GGDGGGGSVTPTSI 66

Query: 63  LPADWSD-SHSD--------HKDDNGVVSRKELEAILFRIA----SEEEVAMMLSEV--D 122
           LP   S  S+ +         +D++G VSR +LE++L R+     +EEE+ +ML EV  D
Sbjct: 67  LPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCD 126

Query: 123 GGGFIRLEALMARAGPAFEPAGES-ELLETFEFFDADHDGRITAEELHGVFSAIGDERCT 182
           G G IRLE L +R   + +PA +S EL ETFEFFDAD DG I+A+EL  VFS IGDERCT
Sbjct: 127 GDGTIRLEELASRV-VSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 186

Query: 183 LEDCRRMIADVDENGDGFVCFAEFVRMMELQR 199
           L+DC+RMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 187 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lag0011927.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 51/128 (39.84%), Postives = 80/128 (62.50%), Query Frame = 0


Query: 75  KDDNGVVSRKELEAILFRI---ASEEEVAMMLSE--VDGGGFIRLEALMARAGPAFEPAG 134
           K+++G +S  EL+ ++  +   AS+EE   M+ E  +DG GFI L+  +A    + + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 135 ES---ELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVC 194
            S   +L E F+ +D D +GRI+A ELH V   +G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of Lag0011927.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 84.7 bits (208), Expect = 8.9e-17
Identity = 51/130 (39.23%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 75  KDDNGVVSRKELEAILFRI---ASEEEVAMMLS--EVDGGGFIRLEALMARAGPAFEPAG 134
           K+ +G +S  EL+ ++  +   AS EE   M+   ++DG GFI L+  +A         G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 135 E-----SELLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGF 194
                 S+L E FE +D D +GRI+A+ELH V   +G E+C+++DC++MI+ VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

[Close]

BLAST of Lag0011927.1 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-14
Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 78  NGVVSRKELEAILFRI---ASEEEVAMMLSEVD--GGGFIRLEALMARAGPAFEPAGESE 137
           +G +S KEL AI+  +     EEE+   ++E+D  G G+I  E  +       +     E
Sbjct: 50  DGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLE 109

Query: 138 -LLETFEFFDADHDGRITAEELHGVFSAIGDERCTLEDCRRMIADVDENGDGFVCFAEFV 197
            L + F  +D D +G I+AEELH V  ++GDE C++ +CR+MI  VD++GDG + F EF 
Sbjct: 110 NLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFK 169

The following BLAST results are available for this feature:

BLAST of Lag0011927.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932888.1	2.1e-76	81.31	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_023543601.1	3.6e-76	81.31	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_022967907.1	4.4e-74	80.81	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_038883626.1	2.0e-71	77.83	probable calcium-binding protein CML35 [Benincasa hispida]	[more]
XP_022153870.1	2.0e-71	78.05	probable calcium-binding protein CML36 [Momordica charantia]	[more]

BLAST of Lag0011927.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30188	1.2e-39	52.91	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9SS31	3.4e-37	51.42	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
Q9C8Y1	1.5e-16	39.84	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	1.3e-15	39.23	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q8RZB5	6.2e-15	39.02	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lag0011927.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F310	1.0e-76	81.31	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	2.1e-74	80.81	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A6J1DI26	9.8e-72	78.05	probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0L0H1	6.4e-71	79.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]
A0A5A7VA05	8.3e-71	79.10	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Lag0011927.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41410.1	8.9e-41	52.91	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	2.4e-38	51.42	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.1e-17	39.84	calmodulin like 23	[more]
AT5G37770.1	8.9e-17	39.23	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.1e-14	37.60	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 132..160 e-value: 1.2E-4 score: 31.5 coord: 169..197 e-value: 4.4E-5 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 131..194 e-value: 4.9E-9 score: 36.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 165..198 score: 12.7281
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 128..163 score: 13.871811
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 132..194 e-value: 1.18834E-17 score: 71.4249
None	No IPR available	GENE3D	1.10.238.10		coord: 71..195 e-value: 8.9E-23 score: 82.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..54
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..79
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..196
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..196
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 141..153
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 178..190
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 75..194

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0011927	Lag0011927	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0011927.1.exon1	Lag0011927.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0011927.1	cds.Lag0011927.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0011927.1	Lag0011927.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding