Lag0011916.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0011916.1 vs. NCBI nr
Match: ONI17050.1 (hypothetical protein PRUPE_3G135000 [Prunus persica])

HSP 1 Score: 93.6 bits (231), Expect = 4.0e-15
Identity = 48/148 (32.43%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 230 LEGGGRGYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQ 289
           L+ G  GYPFTWSNRR E + ++ R D+ + S +    + +AKVS+    GS+H ++LL+
Sbjct: 14  LDLGFVGYPFTWSNRR-EGERIQERLDRALASSSWVHRFGEAKVSHHLVSGSDHAVILLE 73

Query: 290 VAGLRLKYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMK 349
           V  +++ +  RF+Y+  W   + C+ A++ +W     G   Y +VEK++  R  + QW +
Sbjct: 74  VEPVQVTHRGRFIYDHRWGKEVGCRTAVQDSWRWKCRGSACYRLVEKLKAARGNMRQWRR 133

Query: 350 NATVNSGQEIRRLEMTKQFELDRVVPNY 378
            A  N+   IR  E+ K+ ++    P +
Sbjct: 134 GAKTNTQCRIR--EINKELQISYQAPGF 158

BLAST of Lag0011916.1 vs. NCBI nr
Match: KAG5563042.1 (hypothetical protein RHGRI_005701 [Rhododendron griersonianum])

HSP 1 Score: 86.7 bits (213), Expect = 4.9e-13
Identity = 50/174 (28.74%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 215 CFTVKPRGVRRLMFTLEG-------------GGRGYPFTWSNRRKEVDLVKLRSDQFVGS 274
           C   K  GV R  ++L+G             G  GYPFTW N R  +D V+ R D+ + +
Sbjct: 6   CSAEKKGGVVRPAWSLKGFQNFINDCGLVDLGFSGYPFTWHNNRSGIDSVQERLDRVLAT 65

Query: 275 ENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRLKYPKRFVYNRGWRASLECKQAMEGAW 334
            + C  + QA V++L ++GS+H  + L++      +   F ++  W    E +  +  AW
Sbjct: 66  PSWCLSFAQASVTHLDTVGSDHNAIHLKLRDHARGFRIPFRFDARWVEDEEARHVINQAW 125

Query: 335 VGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNSGQEIRRLEMTKQFELDRVVP 376
              ++G   ++V +KIQ  R ++  W +    NSG+ I  L+  K F L    P
Sbjct: 126 ATPIQGSRSFLVFKKIQACRSSLTNWKRRKRDNSGKSIAELK-AKIFSLSNSWP 178

BLAST of Lag0011916.1 vs. NCBI nr
Match: XP_025652024.1 (uncharacterized protein LOC112748031 [Arachis hypogaea])

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-12
Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 236 GYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRL 295
           G PFTWSNRR+  DL+  R D+ +G+E    ++P A V  LT  GS+H  ++L    L  
Sbjct: 178 GRPFTWSNRRRNEDLIMERLDRCLGNEEWTHQYPNAVVLRLTETGSDHAPLILDTNPLME 237

Query: 296 KYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNS 355
           +  +RF +   W    E ++ +   W    +G   YV+ +K++  R  ++ W +    NS
Sbjct: 238 RSKRRFKFQERWCGREEIRETIRLVWETYFDGSAMYVLAQKLKLCRHRLVAWQQTGRSNS 297

Query: 356 GQEIRRLE 364
            + I  LE
Sbjct: 298 KKLIEELE 305

BLAST of Lag0011916.1 vs. NCBI nr
Match: OMO82636.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 82.8 bits (203), Expect = 7.0e-12
Identity = 49/174 (28.16%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 188 PKFDFPVKNKNKATKLETIRRQLNFEGCFTVKPRGVRRLMFTLEGG--GRGYPFTWSNRR 247
           P   F ++ KN+   +E + +   FE  F V P G+   +   E      G  FTW  RR
Sbjct: 433 PDIVFLMETKNQVETIEKLSKTCKFEKKFYVNPDGLSGGLLFGERSLWSEGVRFTWVARR 492

Query: 248 KEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRLKYPKRFVYNR 307
             V L++ R D+ + +       P AKV  L ++GS+H  +L+       K PK F +  
Sbjct: 493 DHV-LIRERLDRALANMEWLELNPNAKVQNLPAVGSDHSPILVLSDLKDKKAPKEFRFES 552

Query: 308 GWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNSGQEI 360
            W    +C   +   W  + +G  GY +V+K++ +R  +++W KN   N+ ++I
Sbjct: 553 MWAEHKDCADVIRKGWQPDYDGSQGYQLVKKLKNSRRALIEWSKNTFPNNRKKI 605

BLAST of Lag0011916.1 vs. NCBI nr
Match: KAG5514982.1 (hypothetical protein RHGRI_036130 [Rhododendron griersonianum])

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-11
Identity = 41/120 (34.17%), Postives = 63/120 (52.50%), Query Frame = 0

Query: 236 GYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRL 295
           G+PFTW+N+R   + V++R D+F+ S +     P A V +L   GSNH  +LL       
Sbjct: 303 GFPFTWNNKRSGRENVRVRLDRFLASSSWRIHHPNAVVKHLAPGGSNHCPILLDSVCQVG 362

Query: 296 KYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNS 355
           ++ +RF+++  W    EC   +  AW   V G   + + EKI+  RM  L W K   +NS
Sbjct: 363 RFKQRFIFDMRWGGYEECAVIVRRAWQRQVHGSRWFQIHEKIKACRMGFLHWRKQRPLNS 422

BLAST of Lag0011916.1 vs. ExPASy TrEMBL
Match: M5VH78 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015748mg PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.9e-15
Identity = 48/148 (32.43%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 230 LEGGGRGYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQ 289
           L+ G  GYPFTWSNRR E + ++ R D+ + S +    + +AKVS+    GS+H ++LL+
Sbjct: 27  LDLGFVGYPFTWSNRR-EGERIQERLDRALASSSWVHRFGEAKVSHHLVSGSDHAVILLE 86

Query: 290 VAGLRLKYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMK 349
           V  +++ +  RF+Y+  W   + C+ A++ +W     G   Y +VEK++  R  + QW +
Sbjct: 87  VEPVQVTHRGRFIYDHRWGKEVGCRTAVQDSWRWKCRGSACYRLVEKLKAARGNMRQWRR 146

Query: 350 NATVNSGQEIRRLEMTKQFELDRVVPNY 378
            A  N+   IR  E+ K+ ++    P +
Sbjct: 147 GAKTNTQCRIR--EINKELQISYQAPGF 171

BLAST of Lag0011916.1 vs. ExPASy TrEMBL
Match: A0A251Q0X9 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G135000 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.9e-15
Identity = 48/148 (32.43%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 230 LEGGGRGYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQ 289
           L+ G  GYPFTWSNRR E + ++ R D+ + S +    + +AKVS+    GS+H ++LL+
Sbjct: 14  LDLGFVGYPFTWSNRR-EGERIQERLDRALASSSWVHRFGEAKVSHHLVSGSDHAVILLE 73

Query: 290 VAGLRLKYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMK 349
           V  +++ +  RF+Y+  W   + C+ A++ +W     G   Y +VEK++  R  + QW +
Sbjct: 74  VEPVQVTHRGRFIYDHRWGKEVGCRTAVQDSWRWKCRGSACYRLVEKLKAARGNMRQWRR 133

Query: 350 NATVNSGQEIRRLEMTKQFELDRVVPNY 378
            A  N+   IR  E+ K+ ++    P +
Sbjct: 134 GAKTNTQCRIR--EINKELQISYQAPGF 158

BLAST of Lag0011916.1 vs. ExPASy TrEMBL
Match: A0A1R3IJA9 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_11842 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.4e-12
Identity = 49/174 (28.16%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 188 PKFDFPVKNKNKATKLETIRRQLNFEGCFTVKPRGVRRLMFTLEGG--GRGYPFTWSNRR 247
           P   F ++ KN+   +E + +   FE  F V P G+   +   E      G  FTW  RR
Sbjct: 433 PDIVFLMETKNQVETIEKLSKTCKFEKKFYVNPDGLSGGLLFGERSLWSEGVRFTWVARR 492

Query: 248 KEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRLKYPKRFVYNR 307
             V L++ R D+ + +       P AKV  L ++GS+H  +L+       K PK F +  
Sbjct: 493 DHV-LIRERLDRALANMEWLELNPNAKVQNLPAVGSDHSPILVLSDLKDKKAPKEFRFES 552

Query: 308 GWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNSGQEI 360
            W    +C   +   W  + +G  GY +V+K++ +R  +++W KN   N+ ++I
Sbjct: 553 MWAEHKDCADVIRKGWQPDYDGSQGYQLVKKLKNSRRALIEWSKNTFPNNRKKI 605

BLAST of Lag0011916.1 vs. ExPASy TrEMBL
Match: A0A5A7P590 (DNAse I-like superfamily protein OS=Striga asiatica OX=4170 GN=STAS_03482 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.8e-12
Identity = 46/144 (31.94%), Postives = 76/144 (52.78%), Query Frame = 0

Query: 235 RGYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLR 294
           +GY +TW N R     V+ R D+  GS     ++  AKV  L    S+H M++L   G +
Sbjct: 32  QGYQYTWVNNRDNEGYVEERLDKAFGSMGWIADYAGAKVLNLYRSASDHGMLILDSDGQQ 91

Query: 295 LKYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVN 354
            K P+RF +++ W     CK+ +E AW     G   +++ E I++TR+ + QW KN  ++
Sbjct: 92  QKKPRRFSFDKRWIKMEGCKETIEAAWDRTHFGTPMFILKESIKQTRVALFQWSKN-FIS 151

Query: 355 SGQEIRRLEMTKQFELDRVVPNYQ 379
            G+E R   +T + E  R +  Y+
Sbjct: 152 QGKE-RIQTLTLKLEEMRALGGYK 173

BLAST of Lag0011916.1 vs. ExPASy TrEMBL
Match: A0A5A7NXH9 (RNA-directed DNA polymerase-like protein OS=Striga asiatica OX=4170 GN=STAS_00537 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 7.6e-12
Identity = 36/125 (28.80%), Postives = 69/125 (55.20%), Query Frame = 0

Query: 236 GYPFTWSNRRKEVDLVKLRSDQFVGSENLCGEWPQAKVSYLTSLGSNHRMVLLQVAGLRL 295
           G+PFTWSN RK  D V+++ D+F+ S +   ++P+  V ++  + S+H ++L+ + G   
Sbjct: 179 GHPFTWSNNRKGKDFVEVQLDRFLLSPDWLLKFPKVSVLHIPLVSSDHSLLLMDIVGTEQ 238

Query: 296 KYPKRFVYNRGWRASLECKQAMEGAWVGNVEGRGGYVVVEKIQRTRMTILQWMKNATVNS 355
           K   RF ++R W       + ++ AW   V G G Y   ++++  +  +L W+ +   NS
Sbjct: 239 KRKHRFYFDRRWAKEEGFDECVKKAWKMQVTGAGLYSFQKRLRNVKFGLLGWLTSTATNS 298

Query: 356 GQEIR 361
            + I+
Sbjct: 299 SKIIK 303