Lag0007089.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0007089.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DEAD-box ATP-dependent RNA helicase
Location	chr6: 48610169 .. 48611766 (-)
Sequence length	573
RNA-Seq Expression	Lag0007089.1
Synteny	Lag0007089.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGCAGCATCAAATTTATGAACGAAGAGATGAAGGCGACGACGAAGAAAATGGTTAAAGAGAGTGAGATTTTTGCTTCTTGTACTTTCGCCAGCCTCGGTTTGCACTCTACTTTATGTGACCAACTCCGAGGTGTGTTGAGTGCCGACTTTGTTCTCTTCCTTCACCGTGTCCATTTATAATCTGCTTTCTACCATGCTAATCACCATAGAATTTTTACTTTAATAGTTTTTTGAGGATTTAGCGTACTCGGTTAATTGCATGGATGATAATCTTGGTCTGCATCTTCCTGTGTGGTTTTTTCTTTGATTGCGACAGATAAAATGGGATTTGAAGTTCCAACCATTGTGCAGGCTCAAGCAATACCGGCTATTTTATCTGGGCGTCACGTGTATCCTTTGCATGCAACTTCTTTTAATTGATATATCCGTGCTTGCAGATGCGTGCGTGTTTATATTTTGATAAGAAACTGATACTATCTTGAAGAAAAAGGCCATACAAAAAGGGGAGATTAGATATCCACTCAAGGATATCGATTCCCTATCTTTATCACGTGTCAATTCATTGAACAGCGCATTTGTCTTTTAATATCTGTAGTGCTTCTCTGTAAGTTTTCTTAACTTCGTTATGTAGTCTTGTTAATGCGGCCACGGGCACTGGCAAAACTGCCGCGTATTTGGTTCCAATCATCCATCACTTGCAGAAGTCTGATCGTAGGATTCAACGAGCCGATGGAACTTTCGGTGCGTAACTTAAGTAATTTTCTTTTCATGCTTGATTCTGTATGTATTATTTAAATTACAAGTTTAGTCATTGAATCTTCAAAGATATGTCTAATAGGTCTATGAACTTTAAACAGTGTCTAAAGGTTCCCTAAACTTTAAATTTGGTGTCTAATTGTTCTTTGCCTTTAGCTCCATCAAACTCCATCAGTTAAGTGCTGATGTGGCACGCTGACTTTGCAATGCAAATGTGTCAAATGAAAATCTTCACATGAAATAAACTTGAATGGGTCTATAACTAAGGCGGGTTTAAAATTGGGGCAGGTTTTAAGTAAATTGGGGCTTCTTCTTTCAGCATATCAAGCTTCAAATTTCAACATTACAAATCCATCCAGCAAGTCCAACAATGGCATTTTTCGCAAAGTGGAAGTTGGAGAAGGGCATAATCACATTGTGAGAGTACTGGGTAGGGATATATGGGGAATAGTGGTAGAATGGTAGAAAGGGTATAATAGTAATTAATTAGGGTGTCTTGGTTGTGTTTTAGTTATAAATAGGTGTTAGGGGTCTTAGAAGGGTGTGGAAGATTTTGGTATTGTCTCATACTGGACTTTGCGAGAGAGTTAGCCCTCTCGAAAGGCTATTGTTATATTGTAGTTCCGTTTAGATATTACAATATATTAGTATTTGAAGTTTTCTTAGTTTGGATACCTGACAAAGTGGTATTAGAGCACGTCACCCTAGGGAGAACACGAAGCATGACTCAGAAACAGTTAGAAGAACGTGTGGAAACAACTGAGAAGGATATTGCCATGTTGAAGGAATCGGTCCAGCAGATTGAGAAGGGAACAAAGAGGCTGTCGTGGAAGTGA

mRNA sequence

ATGAATAGCAGCATCAAATTTATGAACGAAGAGATGAAGGCGACGACGAAGAAAATGGTTAAAGAGAGTGAGATTTTTGCTTCTTGTACTTTCGCCAGCCTCGGTTTGCACTCTACTTTATGTGACCAACTCCGAGATAAAATGGGATTTGAAGTTCCAACCATTGTGCAGGCTCAAGCAATACCGGCTATTTTATCTGGGCGTCACGTTCTTGTTAATGCGGCCACGGGCACTGGCAAAACTGCCGCGTATTTGGTTCCAATCATCCATCACTTGCAGAAGTCTGATCGTAGGATTCAACGAGCCGATGGAACTTTCGCATATCAAGCTTCAAATTTCAACATTACAAATCCATCCAGCAAGTCCAACAATGGCATTTTTCGCAAAGTGGAAGTTGGAGAAGGGCATAATCACATTGTGAGAGTACTGGAGCACGTCACCCTAGGGAGAACACGAAGCATGACTCAGAAACAGTTAGAAGAACGTGTGGAAACAACTGAGAAGGATATTGCCATGTTGAAGGAATCGGTCCAGCAGATTGAGAAGGGAACAAAGAGGCTGTCGTGGAAGTGA

Coding sequence (CDS)

ATGAATAGCAGCATCAAATTTATGAACGAAGAGATGAAGGCGACGACGAAGAAAATGGTTAAAGAGAGTGAGATTTTTGCTTCTTGTACTTTCGCCAGCCTCGGTTTGCACTCTACTTTATGTGACCAACTCCGAGATAAAATGGGATTTGAAGTTCCAACCATTGTGCAGGCTCAAGCAATACCGGCTATTTTATCTGGGCGTCACGTTCTTGTTAATGCGGCCACGGGCACTGGCAAAACTGCCGCGTATTTGGTTCCAATCATCCATCACTTGCAGAAGTCTGATCGTAGGATTCAACGAGCCGATGGAACTTTCGCATATCAAGCTTCAAATTTCAACATTACAAATCCATCCAGCAAGTCCAACAATGGCATTTTTCGCAAAGTGGAAGTTGGAGAAGGGCATAATCACATTGTGAGAGTACTGGAGCACGTCACCCTAGGGAGAACACGAAGCATGACTCAGAAACAGTTAGAAGAACGTGTGGAAACAACTGAGAAGGATATTGCCATGTTGAAGGAATCGGTCCAGCAGATTGAGAAGGGAACAAAGAGGCTGTCGTGGAAGTGA

Protein sequence

MNSSIKFMNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSGRHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFAYQASNFNITNPSSKSNNGIFRKVEVGEGHNHIVRVLEHVTLGRTRSMTQKQLEERVETTEKDIAMLKESVQQIEKGTKRLSWK

Homology

[Close]

BLAST of Lag0007089.1 vs. NCBI nr
Match: XP_022975312.1 (DEAD-box ATP-dependent RNA helicase 17 isoform X2 [Cucurbita maxima])

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-43
Identity = 92/100 (92.00%), Postives = 96/100 (96.00%), Query Frame = 0


Query: 8   MNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSG 67
           MNE MK TTKKMVKE EIFASCTFASLGLH+TLCD+LRDKMGFEVPT+VQAQAIPAILSG
Sbjct: 1   MNEVMKTTTKKMVKEDEIFASCTFASLGLHTTLCDELRDKMGFEVPTLVQAQAIPAILSG 60

Query: 68  RHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           RHVLVNAATGTGKT AYLVPIIHHLQKSDRRIQRADGTF+
Sbjct: 61  RHVLVNAATGTGKTVAYLVPIIHHLQKSDRRIQRADGTFS 100

[Close]

BLAST of Lag0007089.1 vs. NCBI nr
Match: XP_022946513.1 (DEAD-box ATP-dependent RNA helicase 17 isoform X1 [Cucurbita moschata] >XP_023546293.1 DEAD-box ATP-dependent RNA helicase 17 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-43
Identity = 92/100 (92.00%), Postives = 96/100 (96.00%), Query Frame = 0


Query: 8   MNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSG 67
           MNE MK TTKKMVKE EIFASCTFASLGLH+TLCD+LRDKMGFEVPT+VQAQAIPAILSG
Sbjct: 1   MNEGMKTTTKKMVKEDEIFASCTFASLGLHATLCDELRDKMGFEVPTLVQAQAIPAILSG 60

Query: 68  RHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           RHVLVNAATGTGKT AYLVPIIHHLQKSDRRIQRADGTF+
Sbjct: 61  RHVLVNAATGTGKTVAYLVPIIHHLQKSDRRIQRADGTFS 100

[Close]

BLAST of Lag0007089.1 vs. NCBI nr
Match: XP_022946514.1 (DEAD-box ATP-dependent RNA helicase 17 isoform X2 [Cucurbita moschata] >XP_023546294.1 DEAD-box ATP-dependent RNA helicase 17 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-43
Identity = 92/100 (92.00%), Postives = 96/100 (96.00%), Query Frame = 0


Query: 8   MNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSG 67
           MNE MK TTKKMVKE EIFASCTFASLGLH+TLCD+LRDKMGFEVPT+VQAQAIPAILSG
Sbjct: 1   MNEGMKTTTKKMVKEDEIFASCTFASLGLHATLCDELRDKMGFEVPTLVQAQAIPAILSG 60

Query: 68  RHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           RHVLVNAATGTGKT AYLVPIIHHLQKSDRRIQRADGTF+
Sbjct: 61  RHVLVNAATGTGKTVAYLVPIIHHLQKSDRRIQRADGTFS 100

[Close]

BLAST of Lag0007089.1 vs. NCBI nr
Match: XP_022975310.1 (DEAD-box ATP-dependent RNA helicase 17 isoform X1 [Cucurbita maxima])

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-43
Identity = 92/100 (92.00%), Postives = 96/100 (96.00%), Query Frame = 0


Query: 8   MNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSG 67
           MNE MK TTKKMVKE EIFASCTFASLGLH+TLCD+LRDKMGFEVPT+VQAQAIPAILSG
Sbjct: 1   MNEVMKTTTKKMVKEDEIFASCTFASLGLHTTLCDELRDKMGFEVPTLVQAQAIPAILSG 60

Query: 68  RHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           RHVLVNAATGTGKT AYLVPIIHHLQKSDRRIQRADGTF+
Sbjct: 61  RHVLVNAATGTGKTVAYLVPIIHHLQKSDRRIQRADGTFS 100

[Close]

BLAST of Lag0007089.1 vs. NCBI nr
Match: XP_038890369.1 (DEAD-box ATP-dependent RNA helicase 17-like [Benincasa hispida])

HSP 1 Score: 187.2 bits (474), Expect = 1.3e-43
Identity = 94/107 (87.85%), Postives = 99/107 (92.52%), Query Frame = 0


Query: 1   MNSSIKFMNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQA 60
           M+ S K+MNE MK+TTKKMVKE EIFASCTFASLGL STLCDQLRDKMGFEVPT+VQAQA
Sbjct: 1   MSGSTKYMNEGMKSTTKKMVKEDEIFASCTFASLGLDSTLCDQLRDKMGFEVPTLVQAQA 60

Query: 61  IPAILSGRHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           IPAILS RHVLVNAATGTGKT AYLVPIIHH+ KSDRRIQRADGTFA
Sbjct: 61  IPAILSRRHVLVNAATGTGKTVAYLVPIIHHMLKSDRRIQRADGTFA 107

[Close]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot
Match: Q7XJN0 (DEAD-box ATP-dependent RNA helicase 17 OS=Arabidopsis thaliana OX=3702 GN=RH17 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 61/104 (58.65%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 4   SIKFMNEEMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPA 63
           S +   +E K  +K    +S +FASC+F+SLGL + L DQL+++MGFE PT+VQAQAIP 
Sbjct: 7   SARETKQEAKDASK---AKSGLFASCSFSSLGLDTKLSDQLKERMGFEAPTLVQAQAIPV 66

Query: 64  ILSGRHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           ILSGR VLVNA TGTGKT AYL P+IHHLQ    ++ R+ GTFA
Sbjct: 67  ILSGRDVLVNAPTGTGKTIAYLAPLIHHLQGHSPKVDRSHGTFA 107

[Close]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot
Match: Q0DLB9 (DEAD-box ATP-dependent RNA helicase 17 OS=Oryza sativa subsp. japonica OX=39947 GN=RH17 PE=2 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 56/95 (58.95%), Postives = 70/95 (73.68%), Query Frame = 0


Query: 13  KATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSGRHVLV 72
           K+   K   +  +FASC+F  LGLH TLC  L+DKMGF+ PT +QAQAIP  +SG+H+LV
Sbjct: 7   KSPVAKEEDKEGLFASCSFTDLGLHPTLCAHLQDKMGFQAPTRIQAQAIPVAMSGQHMLV 66

Query: 73  NAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
            AATGTGKT AYL PI+H LQ  + R++R DGTFA
Sbjct: 67  KAATGTGKTLAYLAPIVHLLQMREPRVERTDGTFA 101

[Close]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot
Match: Q9H8H2 (Probable ATP-dependent RNA helicase DDX31 OS=Homo sapiens OX=9606 GN=DDX31 PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 5.6e-13
Identity = 40/107 (37.38%), Postives = 61/107 (57.01%), Query Frame = 0


Query: 3   SSIKFMNEEMKATTKKMVK--ESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQA 62
           SS+   N ++    + +VK  + ++F S  F  LGLH  L   +   +     T VQ Q+
Sbjct: 202 SSLFKNNPDIPELHRPVVKQVQEKVFTSAAFHELGLHPHLISTINTVLKMSSMTSVQKQS 261

Query: 63  IPAILSGRHVLVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           IP +L GR  LV + TG+GKT AY +P++  LQ  + +IQR+DG +A
Sbjct: 262 IPVLLEGRDALVRSQTGSGKTLAYCIPVVQSLQAMESKIQRSDGPYA 308

[Close]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot
Match: Q86B47 (Probable ATP-dependent RNA helicase CG8611 OS=Drosophila melanogaster OX=7227 GN=CG8611 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 40/90 (44.44%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 18  KMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSGRHVLVNAATG 77
           K VKE+ IF     ++LGLH      L D +     T VQ + IP +L G+ VLV + TG
Sbjct: 317 KPVKET-IFTGSKISTLGLHPHAVKNLEDLLSIRELTSVQQKTIPEVLQGKDVLVRSQTG 376

Query: 78  TGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           +GKT AY +P++  LQK   RIQR DG  A
Sbjct: 377 SGKTLAYALPLVELLQKQQPRIQRKDGVLA 405

[Close]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot
Match: Q6NZQ2 (Probable ATP-dependent RNA helicase DDX31 OS=Mus musculus OX=10090 GN=Ddx31 PE=2 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 6.2e-12
Identity = 38/97 (39.18%), Postives = 55/97 (56.70%), Query Frame = 0


Query: 11  EMKATTKKMVKESEIFASCTFASLGLHSTLCDQLRDKMGFEVPTIVQAQAIPAILSGRHV 70
           E+ +T  K  +E ++F+   F  L LH  L   +   +     T VQ Q+IP +L GR  
Sbjct: 86  ELHSTVVKQARE-QVFSPEAFQELDLHPHLISTINTVLKMSSMTSVQKQSIPVLLEGRDA 145

Query: 71  LVNAATGTGKTAAYLVPIIHHLQKSDRRIQRADGTFA 108
           LV + TG+GKT AY VP++  LQ    +IQR+DG +A
Sbjct: 146 LVRSQTGSGKTLAYCVPVVQSLQALTSKIQRSDGPYA 181

The following BLAST results are available for this feature:

BLAST of Lag0007089.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022975312.1	1.0e-43	92.00	DEAD-box ATP-dependent RNA helicase 17 isoform X2 [Cucurbita maxima]	[more]
XP_022946513.1	1.0e-43	92.00	DEAD-box ATP-dependent RNA helicase 17 isoform X1 [Cucurbita moschata] >XP_02354...	[more]
XP_022946514.1	1.0e-43	92.00	DEAD-box ATP-dependent RNA helicase 17 isoform X2 [Cucurbita moschata] >XP_02354...	[more]
XP_022975310.1	1.0e-43	92.00	DEAD-box ATP-dependent RNA helicase 17 isoform X1 [Cucurbita maxima]	[more]
XP_038890369.1	1.3e-43	87.85	DEAD-box ATP-dependent RNA helicase 17-like [Benincasa hispida]	[more]

BLAST of Lag0007089.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XJN0	2.6e-26	58.65	DEAD-box ATP-dependent RNA helicase 17 OS=Arabidopsis thaliana OX=3702 GN=RH17 P...	[more]
Q0DLB9	2.6e-26	58.95	DEAD-box ATP-dependent RNA helicase 17 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9H8H2	5.6e-13	37.38	Probable ATP-dependent RNA helicase DDX31 OS=Homo sapiens OX=9606 GN=DDX31 PE=1 ...	[more]
Q86B47	2.1e-12	44.44	Probable ATP-dependent RNA helicase CG8611 OS=Drosophila melanogaster OX=7227 GN...	[more]
Q6NZQ2	6.2e-12	39.18	Probable ATP-dependent RNA helicase DDX31 OS=Mus musculus OX=10090 GN=Ddx31 PE=2...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 156..183
None	No IPR available	PANTHER	PTHR47959	ATP-DEPENDENT RNA HELICASE RHLE-RELATED	coord: 15..98
IPR011545	DEAD/DEAH box helicase domain	PFAM	PF00270	DEAD	coord: 55..101 e-value: 3.3E-12 score: 46.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 2..126 e-value: 2.3E-25 score: 91.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 21..97
IPR014014	RNA helicase, DEAD-box type, Q motif	PROSITE	PS51195	Q_MOTIF	coord: 29..58 score: 9.505568

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0007089	Lag0007089	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0007089.1.exon5	Lag0007089.1.exon5	exon
Lag0007089.1.exon4	Lag0007089.1.exon4	exon
Lag0007089.1.exon3	Lag0007089.1.exon3	exon
Lag0007089.1.exon2	Lag0007089.1.exon2	exon
Lag0007089.1.exon1	Lag0007089.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0007089.1	cds.Lag0007089.1_5	CDS
cds.Lag0007089.1	cds.Lag0007089.1_4	CDS
cds.Lag0007089.1	cds.Lag0007089.1_3	CDS
cds.Lag0007089.1	cds.Lag0007089.1_2	CDS
cds.Lag0007089.1	cds.Lag0007089.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0007089.1	Lag0007089.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042254	ribosome biogenesis
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003724	RNA helicase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003723	RNA binding