Lag0006258.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0006258.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr6: 40149419 .. 40151821 (-)
Sequence length1860
RNA-Seq ExpressionLag0006258.1
SyntenyLag0006258.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAGCACATTCAAATTCAAATCCCAATCGTCGAGCTCTGTCCCAGAAGCTCGTCGCCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATCCACACCCAGATGCTCACCAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGAACTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACCACATGGAATAAGTCTTCTCTTGCCCTTGATTTCTATTCTCGAATGAAACTTCTGGGTCTGAGACCCAATAAACTCACGTATCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGAAGTGGGCGGGTGGGTCATTGTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCGTTGATTACGATGTATGCGAGATGTGGTGAATTGGGTGATGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCTTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGTGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGAATTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGATGGAGAATAAGATGGTGTTGAATTCTTATATGGGTTCTGCGTTGATCCATATGTATGGGAAGTGTGGTGATTTAGCGTCAGCAAGGAGGATCTTTGACTCTATGAAGAAGAAAGATAAAGTTACCTGGAATGCCATGATTACAGGGTAAGTGAATGACCACCTTCTCTTTGAATCAATACGTTTCAATGTTGTTAATTGATGGTAGGTTAAATATATTTTATAGTCCCTATAGTTTAAAACGTTTCAATTTAGTCCTTTACATTTTAAACGATTTTCAGTTACACCATTTTTGTTAGTGGATGTTTAAATATGTCTCATGGTTAACTTATGTGGTATGATACTAAGTGAATTTGCAAAAACTTGAGGAACAATCATTGGAAATGCTGAGAAGAAATTAAAAGCTTTCTGCTCTTAGGTGGTGTAATTCCTCTTCTAAATCTATGCCAACTCTAAGTATCATGTTATGTAAGCATACTATTAGTTAATTTTAATATTCATCAATGGAAATTTAAACACAAGGGACTAAATTGAAACTATTTTCAATTTAAACAATGGAGACTAAATAAGAAAAGAAGCTCAAACATTAGTGACTAAAACATGTCTTTAGTCAAGATAATAATGTATTTCTATATTCCAAAGTTTATGGAATCTTACTTTTTTTTTTTTCCTCCAATCAATCAATTGCAGATATGCACAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGCAGTACAACTCCAGATCAAATAACATTGATAGGAATGCTTTCTGCCTGTGCCTCCATTGGAGCCCTCGACTTGGGGAAGCAAGTAGAGATCTATGCTTCGGAAAGAGGCTTACAGGACGATATTTATGTTGCAACTGCATTGGTTGACATGTATGCGAAATGTGGAAGCTTGGACAATGCATTTCGAGTCTTCGATAGCATGACTAAAAAAAATGAGGTCTCCTGGAATGCTATGATAGCTGCTCTTGCTTTCCATGGCCAAGCTCAAGAAGCCTTGGCACTGTTCAAATCCATGATCAAGGAGGGTGGATCTGCTTCCCCTAATGATATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTTGATGAAGGACGTCGGTTGTTTCACATGATGAGCTCAACATTTAGATTGGTGCCGAAAATTGAGCACTACTCTTGTATGGTTGACCTTTTGTCAAGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCAAGAAGATGCCCGAAAAGGCAGATGAAGTCATTCTTGGAGCATTGCTTGGTGCTTGTCAGAAGCGCAAGAACGTTGACATTAGTGAGCGTGTAATGAATCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCAAGATTATATGCAAATTTGAGAAGATGGGATGATTCAGCTCGGATGAGAATGTTGATGAAGCAAAAGGGTGTTTCTAAGACTCCTGGTTGCAGCTGGATTGATATCAATTCACAACTTCATGAGTTCCATGCTGGTGATGTTCTGCACCAAGAGTGGATTGAAATCCATCAAATCCTAGACTTGTTGATTGATGATTTGAGGAGGGAAGGTTATGTTCCTAATACCAGTCTTGTTTAG

mRNA sequence

ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAGCACATTCAAATTCAAATCCCAATCGTCGAGCTCTGTCCCAGAAGCTCGTCGCCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATCCACACCCAGATGCTCACCAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGAACTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACCACATGGAATAAGTCTTCTCTTGCCCTTGATTTCTATTCTCGAATGAAACTTCTGGGTCTGAGACCCAATAAACTCACGTATCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGAAGTGGGCGGGTGGGTCATTGTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCGTTGATTACGATGTATGCGAGATGTGGTGAATTGGGTGATGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCTTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGTGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGAATTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGATGGAGAATAAGATGGTGTTGAATTCTTATATGGGTTCTGCGTTGATCCATATGTATGGGAAGTGTGGTGATTTAGCGTCAGCAAGGAGGATCTTTGACTCTATGAAGAAGAAAGATAAAGTTACCTGGAATGCCATGATTACAGGATATGCACAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGCAGTACAACTCCAGATCAAATAACATTGATAGGAATGCTTTCTGCCTGTGCCTCCATTGGAGCCCTCGACTTGGGGAAGCAAGTAGAGATCTATGCTTCGGAAAGAGGCTTACAGGACGATATTTATGTTGCAACTGCATTGGTTGACATGTATGCGAAATGTGGAAGCTTGGACAATGCATTTCGAGTCTTCGATAGCATGACTAAAAAAAATGAGGTCTCCTGGAATGCTATGATAGCTGCTCTTGCTTTCCATGGCCAAGCTCAAGAAGCCTTGGCACTGTTCAAATCCATGATCAAGGAGGGTGGATCTGCTTCCCCTAATGATATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTTGATGAAGGACGTCGGTTGTTTCACATGATGAGCTCAACATTTAGATTGGTGCCGAAAATTGAGCACTACTCTTGTATGGTTGACCTTTTGTCAAGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCAAGAAGATGCCCGAAAAGGCAGATGAAGTCATTCTTGGAGCATTGCTTGGTGCTTGTCAGAAGCGCAAGAACGTTGACATTAGTGAGCGTGTAATGAATCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCAAGATTATATGCAAATTTGAGAAGATGGGATGATTCAGCTCGGATGAGAATGTTGATGAAGCAAAAGGGTGTTTCTAAGACTCCTGGTTGCAGCTGGATTGATATCAATTCACAACTTCATGAGTTCCATGCTGGTGATGTTCTGCACCAAGAGTGGATTGAAATCCATCAAATCCTAGACTTGTTGATTGATGATTTGAGGAGGGAAGGTTATGTTCCTAATACCAGTCTTGTTTAG

Coding sequence (CDS)

ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAGCACATTCAAATTCAAATCCCAATCGTCGAGCTCTGTCCCAGAAGCTCGTCGCCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATCCACACCCAGATGCTCACCAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGAACTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACCACATGGAATAAGTCTTCTCTTGCCCTTGATTTCTATTCTCGAATGAAACTTCTGGGTCTGAGACCCAATAAACTCACGTATCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGAAGTGGGCGGGTGGGTCATTGTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCGTTGATTACGATGTATGCGAGATGTGGTGAATTGGGTGATGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCTTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGTGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGAATTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGATGGAGAATAAGATGGTGTTGAATTCTTATATGGGTTCTGCGTTGATCCATATGTATGGGAAGTGTGGTGATTTAGCGTCAGCAAGGAGGATCTTTGACTCTATGAAGAAGAAAGATAAAGTTACCTGGAATGCCATGATTACAGGATATGCACAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGCAGTACAACTCCAGATCAAATAACATTGATAGGAATGCTTTCTGCCTGTGCCTCCATTGGAGCCCTCGACTTGGGGAAGCAAGTAGAGATCTATGCTTCGGAAAGAGGCTTACAGGACGATATTTATGTTGCAACTGCATTGGTTGACATGTATGCGAAATGTGGAAGCTTGGACAATGCATTTCGAGTCTTCGATAGCATGACTAAAAAAAATGAGGTCTCCTGGAATGCTATGATAGCTGCTCTTGCTTTCCATGGCCAAGCTCAAGAAGCCTTGGCACTGTTCAAATCCATGATCAAGGAGGGTGGATCTGCTTCCCCTAATGATATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTTGATGAAGGACGTCGGTTGTTTCACATGATGAGCTCAACATTTAGATTGGTGCCGAAAATTGAGCACTACTCTTGTATGGTTGACCTTTTGTCAAGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCAAGAAGATGCCCGAAAAGGCAGATGAAGTCATTCTTGGAGCATTGCTTGGTGCTTGTCAGAAGCGCAAGAACGTTGACATTAGTGAGCGTGTAATGAATCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCAAGATTATATGCAAATTTGAGAAGATGGGATGATTCAGCTCGGATGAGAATGTTGATGAAGCAAAAGGGTGTTTCTAAGACTCCTGGTTGCAGCTGGATTGATATCAATTCACAACTTCATGAGTTCCATGCTGGTGATGTTCTGCACCAAGAGTGGATTGAAATCCATCAAATCCTAGACTTGTTGATTGATGATTTGAGGAGGGAAGGTTATGTTCCTAATACCAGTCTTGTTTAG

Protein sequence

MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKLLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQILDLLIDDLRREGYVPNTSLV
Homology
BLAST of Lag0006258.1 vs. NCBI nr
Match: XP_023521362.1 (pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp. pepo] >XP_023545668.1 pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 565/619 (91.28%), Postives = 598/619 (96.61%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+ALS+KLV FLQQC++IKQLKQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQALSEKLVTFLQQCVSIKQLKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASDFFSKILEPTEYTFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV++N+MVLNSYMGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           QD+IYV+TALVDMYAKCGSLDNAFRVFD M +KNEVSWNAMI+ALAFHGQAQ+ALALFKS
Sbjct: 361 QDNIYVSTALVDMYAKCGSLDNAFRVFDIMPRKNEVSWNAMISALAFHGQAQKALALFKS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           MI EGG ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MINEGGDASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMELLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. NCBI nr
Match: XP_022929625.1 (pentatricopeptide repeat-containing protein At2g34400 [Cucurbita moschata])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 563/619 (90.95%), Postives = 599/619 (96.77%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+A S+KLV FLQQC++IKQLKQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQAFSEKLVTFLQQCVSIKQLKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV++N+MVLNSYMGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +D+IYV+TALVDMYAKCGSLDNAFRVFD M +KNEVSWNAMI+ALAFHGQAQ+ALALF+S
Sbjct: 361 RDNIYVSTALVDMYAKCGSLDNAFRVFDVMPQKNEVSWNAMISALAFHGQAQKALALFES 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           MIKEGG+ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MIKEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. NCBI nr
Match: XP_022997564.1 (pentatricopeptide repeat-containing protein At2g34400 [Cucurbita maxima])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 561/619 (90.63%), Postives = 597/619 (96.45%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+ALS+KLV FLQQCL+IKQ+KQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQALSEKLVTFLQQCLSIKQMKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLF +MM+AGFEPNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFSDMMQAGFEPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFVM+N+MVLNSYMGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVMDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +D+IYV+TALVDMY+KCGSLDNAFRVFD M +KNEVSWNAMI+ALAFHGQAQ+A+ALF S
Sbjct: 361 RDNIYVSTALVDMYSKCGSLDNAFRVFDVMPRKNEVSWNAMISALAFHGQAQKAMALFNS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           M+ EGG+ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MLNEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. NCBI nr
Match: XP_038889591.1 (pentatricopeptide repeat-containing protein At2g34400 [Benincasa hispida])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 562/619 (90.79%), Postives = 596/619 (96.28%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIKASPHLISRY NF+K +S  +PNR+ALS+KL +FLQQC++IKQLKQIHTQMLTNSI
Sbjct: 1   MLRIKASPHLISRYFNFNKTNSKPSPNRQALSEKLNSFLQQCVSIKQLKQIHTQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYKIV+LKDFAY+ DFFSKI+EPTEYSFNVMIRGL+ TWNKSSLAL+ Y RMK 
Sbjct: 61  HKPNSFLYKIVDLKDFAYSLDFFSKIVEPTEYSFNVMIRGLAKTWNKSSLALELYYRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGHCSVIKRGLDEDGHVSHSLITMYARCG++GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHCSVIKRGLDEDGHVSHSLITMYARCGQVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSW+SMISGYSKMR+AGEAVGLFREMMEAGF+PNEM LVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWSSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMALVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV+ENKMVLNS+MGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMS+EAIKLFQDMRMSST PDQITLIG+LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSDEAIKLFQDMRMSSTAPDQITLIGILSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +DDIYVATALVDMYAKCGSLDNAFRVF  M +KNEVSWNAMI+ALAFHGQAQEALALFKS
Sbjct: 361 RDDIYVATALVDMYAKCGSLDNAFRVFYGMPRKNEVSWNAMISALAFHGQAQEALALFKS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           MI EGG+ASPNDITFVGVLSACVHAGLVDEGRRLFHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MINEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKN+DISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIRQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PN SL+
Sbjct: 601 DLLIDDLRREGYIPNISLL 619

BLAST of Lag0006258.1 vs. NCBI nr
Match: KAG6598886.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 559/619 (90.31%), Postives = 597/619 (96.45%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+A S+KLV FLQQC++IKQLKQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQAFSEKLVTFLQQCVSIKQLKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYAS FFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASAFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV++N+MVLNSYMGSALIHMYGKCGDL SARRIFDS+KKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSLKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +D+IYV+TALVDMYAKCGSLD AFRVFD M +KNEVSWNAMI+ALAFHGQAQ+ALALF+S
Sbjct: 361 RDNIYVSTALVDMYAKCGSLDYAFRVFDVMPQKNEVSWNAMISALAFHGQAQKALALFES 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           MIKEGG+ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MIKEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRW+DSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWEDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. ExPASy Swiss-Prot
Match: O64705 (Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E23 PE=3 SV=2)

HSP 1 Score: 659.8 bits (1701), Expect = 3.0e-188
Identity = 336/613 (54.81%), Postives = 433/613 (70.64%), Query Frame = 0

Query: 4   IKASPHLISRYLNFD--KAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSIH 63
           IK      S Y  F   K        R  L +  +  L++C+++ QL+QI  QML +S+ 
Sbjct: 7   IKPEKLAFSIYRQFPKFKPRQFEEARRGDLERDFLFLLKKCISVNQLRQIQAQMLLHSVE 66

Query: 64  KPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKLL 123
           KPN  + K VEL DF Y+S  FS   EP  YSFN MIRGL+ TWN    AL  Y RMK  
Sbjct: 67  KPNFLIPKAVELGDFNYSSFLFSVTEEPNHYSFNYMIRGLTNTWNDHEAALSLYRRMKFS 126

Query: 124 GLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGDA 183
           GL+P+K TY F+FIAC+ L  +G GR  H S+ K GL+ D H++HSLI MYA+CG++G A
Sbjct: 127 GLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQVGYA 186

Query: 184 RKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGEL 243
           RK+FDEI ERD VSWNSMISGYS+  YA +A+ LFR+M E GFEP+E TLVS+LGAC  L
Sbjct: 187 RKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGACSHL 246

Query: 244 GELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAMI 303
           G+L+ G  +EE  +  K+ L++++GS LI MYGKCGDL SARR+F+ M KKD+V W AMI
Sbjct: 247 GDLRTGRLLEEMAITKKIGLSTFLGSKLISMYGKCGDLDSARRVFNQMIKKDRVAWTAMI 306

Query: 304 TGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLQ 363
           T Y+QNG S EA KLF +M  +  +PD  TL  +LSAC S+GAL+LGKQ+E +ASE  LQ
Sbjct: 307 TVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQIETHASELSLQ 366

Query: 364 DDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKSM 423
            +IYVAT LVDMY KCG ++ A RVF++M  KNE +WNAMI A A  G A+EAL LF  M
Sbjct: 367 HNIYVATGLVDMYGKCGRVEEALRVFEAMPVKNEATWNAMITAYAHQGHAKEALLLFDRM 426

Query: 424 IKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRA 483
                S  P+DITF+GVLSACVHAGLV +G R FH MSS F LVPKIEHY+ ++DLLSRA
Sbjct: 427 -----SVPPSDITFIGVLSACVHAGLVHQGCRYFHEMSSMFGLVPKIEHYTNIIDLLSRA 486

Query: 484 GHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLEL-EPSNSGNYVIS 543
           G L+EAW+F+++ P K DE++L A+LGAC KRK+V I E+ M +L+E+ E  N+GNYVIS
Sbjct: 487 GMLDEAWEFMERFPGKPDEIMLAAILGACHKRKDVAIREKAMRMLMEMKEAKNAGNYVIS 546

Query: 544 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAG-DVLHQEWIEIHQI 603
           S + A+++ WD+SA+MR LM+ +GV KTPGCSWI+I  +L EF AG D L     +   +
Sbjct: 547 SNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDSGSL 606

Query: 604 LDLLIDDLRREGY 613
            DLL+++++RE Y
Sbjct: 607 FDLLVEEMKRERY 614

BLAST of Lag0006258.1 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.6e-123
Identity = 240/624 (38.46%), Postives = 366/624 (58.65%), Query Frame = 0

Query: 36  VAFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVE-------LKDFAYASDFFSKILE 95
           ++ L  C T++ L+ IH QM+   +H  N  L K++E        +   YA   F  I E
Sbjct: 37  LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQE 96

Query: 96  PTEYSFNVMIRGLSTTWNKSSLALDFYSRMKLLGLRPNKLTYPFLFIACSNLLAVGSGRV 155
           P    +N M RG + + +  S AL  Y  M  LGL PN  T+PF+  +C+   A   G+ 
Sbjct: 97  PNLLIWNTMFRGHALSSDPVS-ALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 156

Query: 156 GHCSVIKRGLDEDGHVSHSLITMYARCGELGDARKV------------------------ 215
            H  V+K G D D +V  SLI+MY + G L DA KV                        
Sbjct: 157 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 216

Query: 216 -------FDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 275
                  FDEIP +D+VSWN+MISGY++     EA+ LF++MM+    P+E T+V+V+ A
Sbjct: 217 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 276

Query: 276 CGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTW 335
           C + G ++LG  V  ++ ++    N  + +ALI +Y KCG+L +A  +F+ +  KD ++W
Sbjct: 277 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 336

Query: 336 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 395
           N +I GY    + +EA+ LFQ+M  S  TP+ +T++ +L ACA +GA+D+G+ + +Y  +
Sbjct: 337 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 396

Query: 396 R--GLQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEAL 455
           R  G+ +   + T+L+DMYAKCG ++ A +VF+S+  K+  SWNAMI   A HG+A  + 
Sbjct: 397 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASF 456

Query: 456 ALFKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMV 515
            LF  M K G    P+DITFVG+LSAC H+G++D GR +F  M+  +++ PK+EHY CM+
Sbjct: 457 DLFSRMRKIG--IQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 516

Query: 516 DLLSRAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSG 575
           DLL  +G  +EA + +  M  + D VI  +LL AC+   NV++ E     L+++EP N G
Sbjct: 517 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 576

Query: 576 NYVISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIE 620
           +YV+ S +YA+  RW++ A+ R L+  KG+ K PGCS I+I+S +HEF  GD  H    E
Sbjct: 577 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 636

BLAST of Lag0006258.1 vs. ExPASy Swiss-Prot
Match: Q9LTV8 (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 7.2e-118
Identity = 218/575 (37.91%), Postives = 346/575 (60.17%), Query Frame = 0

Query: 47  QLKQIHTQMLTNSIHKPNSFLYKIVELK----DFAYASDFFSKILEPTEYSFNVMIRGLS 106
           QLKQIH ++L   +      + K++       D  +A   F  +  P  + +N +IRG S
Sbjct: 36  QLKQIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYS 95

Query: 107 TTWNKSSLALDFYSRMKLLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDG 166
              N    AL  YS M+L  + P+  T+P L  ACS L  +  GR  H  V + G D D 
Sbjct: 96  RN-NHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADV 155

Query: 167 HVSHSLITMYARCGELGDARKVFD--EIPERDLVSWNSMISGYSKMRYAGEAVGLFREMM 226
            V + LI +YA+C  LG AR VF+   +PER +VSW +++S Y++     EA+ +F +M 
Sbjct: 156 FVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMR 215

Query: 227 EAGFEPNEMTLVSVLGACGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLA 286
           +   +P+ + LVSVL A   L +LK G  +   V++  + +   +  +L  MY KCG +A
Sbjct: 216 KMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVA 275

Query: 287 SARRIFDSMKKKDKVTWNAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACA 346
           +A+ +FD MK  + + WNAMI+GYA+NG + EAI +F +M      PD I++   +SACA
Sbjct: 276 TAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACA 335

Query: 347 SIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNA 406
            +G+L+  + +  Y      +DD+++++AL+DM+AKCGS++ A  VFD    ++ V W+A
Sbjct: 336 QVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSA 395

Query: 407 MIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSS 466
           MI     HG+A+EA++L+++M  E G   PND+TF+G+L AC H+G+V EG   F+ M+ 
Sbjct: 396 MIVGYGLHGRAREAISLYRAM--ERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMAD 455

Query: 467 TFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISE 526
             ++ P+ +HY+C++DLL RAGHL++A++ +K MP +    + GALL AC+K ++V++ E
Sbjct: 456 -HKINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGE 515

Query: 527 RVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQL 586
                L  ++PSN+G+YV  S LYA  R WD  A +R+ MK+KG++K  GCSW+++  +L
Sbjct: 516 YAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRL 575

Query: 587 HEFHAGDVLHQEWIEIHQILDLLIDDLRREGYVPN 616
             F  GD  H  + EI + ++ +   L+  G+V N
Sbjct: 576 EAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVAN 606

BLAST of Lag0006258.1 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.7e-117
Identity = 228/652 (34.97%), Postives = 372/652 (57.06%), Query Frame = 0

Query: 6   ASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQML-TNSIHKPN 65
           A P  + R+ NF   +  +  N R+   + ++ +++C++++QLKQ H  M+ T +   P 
Sbjct: 7   AQPLSLPRHPNFSNPNQPTTNNERS---RHISLIERCVSLRQLKQTHGHMIRTGTFSDPY 66

Query: 66  SF--LYKIVELKDFA---YASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMK 125
           S   L+ +  L  FA   YA   F +I +P  +++N +IR  ++  +       F   + 
Sbjct: 67  SASKLFAMAALSSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDMVS 126

Query: 126 LLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELG 185
                PNK T+PFL  A + + ++  G+  H   +K  +  D  V++SLI  Y  CG+L 
Sbjct: 127 ESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLD 186

Query: 186 DARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACG 245
            A KVF  I E+D+VSWNSMI+G+ +     +A+ LF++M     + + +T+V VL AC 
Sbjct: 187 SACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACA 246

Query: 246 ELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVT--- 305
           ++  L+ G  V  ++ EN++ +N  + +A++ MY KCG +  A+R+FD+M++KD VT   
Sbjct: 247 KIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTT 306

Query: 306 ----------------------------WNAMITGYAQNGMSEEAIKLFQDMRMSSTTP- 365
                                       WNA+I+ Y QNG   EA+ +F ++++      
Sbjct: 307 MLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNMKL 366

Query: 366 DQITLIGMLSACASIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLDNAFRVF 425
           +QITL+  LSACA +GAL+LG+ +  Y  + G++ + +V +AL+ MY+KCG L+ +  VF
Sbjct: 367 NQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSREVF 426

Query: 426 DSMTKKNEVSWNAMIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSACVHAGL 485
           +S+ K++   W+AMI  LA HG   EA+ +F  M  +  +  PN +TF  V  AC H GL
Sbjct: 427 NSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKM--QEANVKPNGVTFTNVFCACSHTGL 486

Query: 486 VDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEVILGALL 545
           VDE   LFH M S + +VP+ +HY+C+VD+L R+G+LE+A  F++ MP      + GALL
Sbjct: 487 VDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALL 546

Query: 546 GACQKRKNVDISERVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMKQKGVSK 605
           GAC+   N++++E     LLELEP N G +V+ S +YA L +W++ + +R  M+  G+ K
Sbjct: 547 GACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKK 606

Query: 606 TPGCSWIDINSQLHEFHAGDVLHQEWIEIHQILDLLIDDLRREGYVPNTSLV 620
            PGCS I+I+  +HEF +GD  H    +++  L  +++ L+  GY P  S V
Sbjct: 607 EPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEISQV 653

BLAST of Lag0006258.1 vs. ExPASy Swiss-Prot
Match: Q9SJZ3 (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 6.1e-117
Identity = 241/642 (37.54%), Postives = 367/642 (57.17%), Query Frame = 0

Query: 3   RIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI-- 62
           R ++ PH   + +N++  HS        L   L++ L++C  +  LKQI  QM+ N +  
Sbjct: 30  RTRSLPHHRDKPINWNSTHS------FVLHNPLLSLLEKCKLLLHLKQIQAQMIINGLIL 89

Query: 63  -HKPNSFLYKIVELKDFAYASDFFSKILE----PTEYSFNVMIRGLSTTWNKSSLALDFY 122
               +S L     L +  Y  D+  KIL+    P  +S+NV IRG S + N     L  Y
Sbjct: 90  DPFASSRLIAFCALSESRYL-DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFL-LY 149

Query: 123 SRMKLLGL---RPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMY 182
            +M   G    RP+  TYP LF  C++L     G +    V+K  L+   HV ++ I M+
Sbjct: 150 KQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGHMILGHVLKLRLELVSHVHNASIHMF 209

Query: 183 ARCGELGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLV 242
           A CG++ +ARKVFDE P RDLVSWN +I+GY K+  A +A+ +++ M   G +P+++T++
Sbjct: 210 ASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDDVTMI 269

Query: 243 SVLGACGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKK 302
            ++ +C  LG+L  G    E+V EN + +   + +AL+ M+ KCGD+  ARRIFD+++K+
Sbjct: 270 GLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDNLEKR 329

Query: 303 DKVTWNAMITGYAQNGM-------------------------------SEEAIKLFQDMR 362
             V+W  MI+GYA+ G+                                ++A+ LFQ+M+
Sbjct: 330 TIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALFQEMQ 389

Query: 363 MSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLD 422
            S+T PD+IT+I  LSAC+ +GALD+G  +  Y  +  L  ++ + T+LVDMYAKCG++ 
Sbjct: 390 TSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSLVDMYAKCGNIS 449

Query: 423 NAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSA 482
            A  VF  +  +N +++ A+I  LA HG A  A++ F  MI  G   +P++ITF+G+LSA
Sbjct: 450 EALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAG--IAPDEITFIGLLSA 509

Query: 483 CVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEV 542
           C H G++  GR  F  M S F L P+++HYS MVDLL RAG LEEA   ++ MP +AD  
Sbjct: 510 CCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPMEADAA 569

Query: 543 ILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMK 602
           + GALL  C+   NV++ E+    LLEL+PS+SG YV+   +Y     W+D+ R R +M 
Sbjct: 570 VWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANMWEDAKRARRMMN 629

Query: 603 QKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQILDLL 604
           ++GV K PGCS I++N  + EF   D    E  +I+  L  L
Sbjct: 630 ERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCL 661

BLAST of Lag0006258.1 vs. ExPASy TrEMBL
Match: A0A6J1EUW3 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita moschata OX=3662 GN=LOC111436152 PE=4 SV=1)

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 563/619 (90.95%), Postives = 599/619 (96.77%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+A S+KLV FLQQC++IKQLKQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQAFSEKLVTFLQQCVSIKQLKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV++N+MVLNSYMGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +D+IYV+TALVDMYAKCGSLDNAFRVFD M +KNEVSWNAMI+ALAFHGQAQ+ALALF+S
Sbjct: 361 RDNIYVSTALVDMYAKCGSLDNAFRVFDVMPQKNEVSWNAMISALAFHGQAQKALALFES 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           MIKEGG+ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MIKEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. ExPASy TrEMBL
Match: A0A6J1KBU4 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita maxima OX=3661 GN=LOC111492454 PE=4 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 561/619 (90.63%), Postives = 597/619 (96.45%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIK+SPHLISRYLNFDK HS  NPNR+ALS+KLV FLQQCL+IKQ+KQIH QMLTNSI
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHSKPNPNRQALSEKLVTFLQQCLSIKQMKQIHNQMLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYK+V+LKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCGE+GD
Sbjct: 121 LGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEVGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLF +MM+AGFEPNEMTLVSVLGACGE
Sbjct: 181 ARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFSDMMQAGFEPNEMTLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFVM+N+MVLNSYMGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVMDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASERGL
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASERGL 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +D+IYV+TALVDMY+KCGSLDNAFRVFD M +KNEVSWNAMI+ALAFHGQAQ+A+ALF S
Sbjct: 361 RDNIYVSTALVDMYSKCGSLDNAFRVFDVMPRKNEVSWNAMISALAFHGQAQKAMALFNS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           M+ EGG+ASPNDITFVGVLSACVHAGLVDEGRR FHMMSS+F LVPKIEHYSCMVDLLSR
Sbjct: 421 MLNEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLLSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFVKKMPEK DEVILGALLGACQKRKNV+ISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEI QIL
Sbjct: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PNT+L+
Sbjct: 601 DLLIDDLRREGYIPNTTLL 619

BLAST of Lag0006258.1 vs. ExPASy TrEMBL
Match: A0A6J1DIG6 (pentatricopeptide repeat-containing protein At2g34400 OS=Momordica charantia OX=3673 GN=LOC111020815 PE=4 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 565/621 (90.98%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAF-LQQCLTIKQLKQIHTQMLTNS 60
           MLRIKASPHLISRYLN +K  +N NPNRRALS+KL+AF LQQCL+++QLKQIHTQM+TNS
Sbjct: 1   MLRIKASPHLISRYLNLEK--TNPNPNRRALSEKLLAFLLQQCLSLRQLKQIHTQMITNS 60

Query: 61  IHKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMK 120
           IHKPNSFL+KI +LKDFAYASDFFS+ILEPTEY+FNVMIRGLSTTWNKSSLAL+ Y RMK
Sbjct: 61  IHKPNSFLFKIADLKDFAYASDFFSRILEPTEYAFNVMIRGLSTTWNKSSLALELYFRMK 120

Query: 121 LLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELG 180
            LGL+PNKLTYPFLFIACSNLLAV  GRVGH SV+KRG DEDGHVSHSLITMYARCGELG
Sbjct: 121 FLGLKPNKLTYPFLFIACSNLLAVEYGRVGHSSVVKRGFDEDGHVSHSLITMYARCGELG 180

Query: 181 DARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACG 240
           DARKVFDEIPERDLVSWNSMISGYSKMRYA EAVGLFREMM AGFEPNEMTLVSVLGACG
Sbjct: 181 DARKVFDEIPERDLVSWNSMISGYSKMRYAAEAVGLFREMMGAGFEPNEMTLVSVLGACG 240

Query: 241 ELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNA 300
           ELG+LKLGTWVEEFV+ENKMVLNS+MGSALIHMYGKCGDL SARRIF+SMKKKDKVTWNA
Sbjct: 241 ELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFESMKKKDKVTWNA 300

Query: 301 MITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERG 360
           MITGYAQNG+SEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQ EIYASERG
Sbjct: 301 MITGYAQNGLSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQAEIYASERG 360

Query: 361 LQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFK 420
           L+DDIYVATALVDMYAKCGSLDNAFRVF+ M +KNEVSWNAMI+ALAFHGQAQEALALFK
Sbjct: 361 LRDDIYVATALVDMYAKCGSLDNAFRVFNGMPRKNEVSWNAMISALAFHGQAQEALALFK 420

Query: 421 SMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLS 480
           SMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMM S+FRLVPKIEHYSCMVDLLS
Sbjct: 421 SMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMCSSFRLVPKIEHYSCMVDLLS 480

Query: 481 RAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVI 540
           RAGHLEEAWDF++KMPEK DEVILGALLGACQKRKN+DISERVM LLLELEPSNSGNYVI
Sbjct: 481 RAGHLEEAWDFIEKMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNYVI 540

Query: 541 SSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHA-GDVLHQEWIEIHQ 600
           SSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDI+SQLHEFHA GDVLH EWIEIHQ
Sbjct: 541 SSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDIDSQLHEFHAGGDVLHHEWIEIHQ 600

Query: 601 ILDLLIDDLRREGYVPNTSLV 620
           IL+LLIDDLRREGY+PNTSL+
Sbjct: 601 ILNLLIDDLRREGYIPNTSLL 619

BLAST of Lag0006258.1 vs. ExPASy TrEMBL
Match: A0A0A0LMG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060420 PE=4 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 549/619 (88.69%), Postives = 581/619 (93.86%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLR KASPHLISRYLN  K HS  NPNR+ALS K  + LQQCL+IKQLKQIH Q+LTNSI
Sbjct: 1   MLRTKASPHLISRYLNLQKPHSKPNPNRQALSDKFNSLLQQCLSIKQLKQIHAQLLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           HKPNSFLYKI +LKDFAYAS FFS IL+PTEYSFNVMIRGLST WNKSSLAL+FYSRMK 
Sbjct: 61  HKPNSFLYKIADLKDFAYASVFFSNILDPTEYSFNVMIRGLSTAWNKSSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PN LTYPFLFIACSNLLAV +GR+GHCSVI+RGLDEDGHVSHSLITMYARCG++GD
Sbjct: 121 LGLKPNNLTYPFLFIACSNLLAVENGRMGHCSVIRRGLDEDGHVSHSLITMYARCGKMGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEI ++DLVSWNSMISGYSKMR+AGEAVGLFREMMEAGF+PNEM+LVSVLGACGE
Sbjct: 181 ARKVFDEISQKDLVSWNSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMSLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV+ENKM LN +MGSALIHMYGKCGDL SARRIFDSMKKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVENKMTLNYFMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMRMSST PDQITLIG+LSACASIGALDLGKQVEIYASERG 
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRMSSTAPDQITLIGILSACASIGALDLGKQVEIYASERGF 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           QDD+YV TALVDMYAKCGSLDNAFRVF  M  KNEVSWNAMI+ALAFHGQAQEALALFKS
Sbjct: 361 QDDVYVGTALVDMYAKCGSLDNAFRVFYGMPNKNEVSWNAMISALAFHGQAQEALALFKS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           M+ EGG+ SPNDITFVGVLSACVHAGLVDEGRRLFHMMSS+F LVPKIEHYSCMVDL SR
Sbjct: 421 MMNEGGTVSPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLFSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDFV  MPEK DEVILGALLGACQKRKN+DISERVM LLLELEPSNSGNYVIS
Sbjct: 481 AGHLEEAWDFVMTMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           S+LYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL
Sbjct: 541 SKLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PN +L+
Sbjct: 601 DLLIDDLRREGYIPNANLL 619

BLAST of Lag0006258.1 vs. ExPASy TrEMBL
Match: A0A1S3BRJ2 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucumis melo OX=3656 GN=LOC103492726 PE=4 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 544/619 (87.88%), Postives = 580/619 (93.70%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI 60
           MLRIKASPHLISRY N  K HS  NPN +ALS+KL + LQQCL+I+QLKQIHTQ+LTNSI
Sbjct: 1   MLRIKASPHLISRYPNLQKPHSKPNPNPQALSEKLNSLLQQCLSIRQLKQIHTQLLTNSI 60

Query: 61  HKPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKL 120
           +KPNSFLYKIV+LKDFAYAS FFS ILEPTEYSFNVMIRGLST WNK SLAL+FYSRMK 
Sbjct: 61  YKPNSFLYKIVDLKDFAYASVFFSNILEPTEYSFNVMIRGLSTAWNKPSLALEFYSRMKF 120

Query: 121 LGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGD 180
           LGL+PNK TYPFLFIACSNLLAV +GR+GHCS IKRGLDEDGHVSHSLITMYARCG++GD
Sbjct: 121 LGLKPNKFTYPFLFIACSNLLAVDNGRMGHCSGIKRGLDEDGHVSHSLITMYARCGKMGD 180

Query: 181 ARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGE 240
           ARKVFDEI ++DLVSWNSMISGYSKMR+AGEAVGLFREMMEAGF+PNEM+LVSVLGACGE
Sbjct: 181 ARKVFDEISQKDLVSWNSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMSLVSVLGACGE 240

Query: 241 LGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAM 300
           LG+LKLGTWVEEFV+ENKM LNS+MGSALIHMYGKCGDL SARRIFDS KKKDKVTWNAM
Sbjct: 241 LGDLKLGTWVEEFVVENKMPLNSFMGSALIHMYGKCGDLVSARRIFDSTKKKDKVTWNAM 300

Query: 301 ITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGL 360
           ITGYAQNGMSEEAIKLFQDMR+SST PDQITLIG+LSACASIGALDLGKQ+EIYASERG 
Sbjct: 301 ITGYAQNGMSEEAIKLFQDMRVSSTAPDQITLIGILSACASIGALDLGKQLEIYASERGY 360

Query: 361 QDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKS 420
           +DDIYV TALVDMYAKCGSLDNAFRVF  M KKNEVSWNAMI+ALAFHGQAQEALALFKS
Sbjct: 361 RDDIYVGTALVDMYAKCGSLDNAFRVFYGMPKKNEVSWNAMISALAFHGQAQEALALFKS 420

Query: 421 MIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSR 480
           M+ EGG+ SPNDITFVGVLSACVHAGLVDEGRRLFHMMSS+F LVPKIEHYSCMVDL SR
Sbjct: 421 MMNEGGTVSPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLFSR 480

Query: 481 AGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVIS 540
           AGHLEEAWDF+  MPEK DEVILGALLGACQKRKN+DISERVMNLLLELEPSNS NYVIS
Sbjct: 481 AGHLEEAWDFIMAMPEKPDEVILGALLGACQKRKNIDISERVMNLLLELEPSNSANYVIS 540

Query: 541 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600
           S+LYA L RWDDSA MRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL
Sbjct: 541 SKLYAKLGRWDDSAMMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQIL 600

Query: 601 DLLIDDLRREGYVPNTSLV 620
           DLLIDDLRREGY+PN +L+
Sbjct: 601 DLLIDDLRREGYIPNANLL 619

BLAST of Lag0006258.1 vs. TAIR 10
Match: AT2G34400.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 659.8 bits (1701), Expect = 2.1e-189
Identity = 336/613 (54.81%), Postives = 433/613 (70.64%), Query Frame = 0

Query: 4   IKASPHLISRYLNFD--KAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSIH 63
           IK      S Y  F   K        R  L +  +  L++C+++ QL+QI  QML +S+ 
Sbjct: 7   IKPEKLAFSIYRQFPKFKPRQFEEARRGDLERDFLFLLKKCISVNQLRQIQAQMLLHSVE 66

Query: 64  KPNSFLYKIVELKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMKLL 123
           KPN  + K VEL DF Y+S  FS   EP  YSFN MIRGL+ TWN    AL  Y RMK  
Sbjct: 67  KPNFLIPKAVELGDFNYSSFLFSVTEEPNHYSFNYMIRGLTNTWNDHEAALSLYRRMKFS 126

Query: 124 GLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELGDA 183
           GL+P+K TY F+FIAC+ L  +G GR  H S+ K GL+ D H++HSLI MYA+CG++G A
Sbjct: 127 GLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQVGYA 186

Query: 184 RKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGEL 243
           RK+FDEI ERD VSWNSMISGYS+  YA +A+ LFR+M E GFEP+E TLVS+LGAC  L
Sbjct: 187 RKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGACSHL 246

Query: 244 GELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTWNAMI 303
           G+L+ G  +EE  +  K+ L++++GS LI MYGKCGDL SARR+F+ M KKD+V W AMI
Sbjct: 247 GDLRTGRLLEEMAITKKIGLSTFLGSKLISMYGKCGDLDSARRVFNQMIKKDRVAWTAMI 306

Query: 304 TGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLQ 363
           T Y+QNG S EA KLF +M  +  +PD  TL  +LSAC S+GAL+LGKQ+E +ASE  LQ
Sbjct: 307 TVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQIETHASELSLQ 366

Query: 364 DDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKSM 423
            +IYVAT LVDMY KCG ++ A RVF++M  KNE +WNAMI A A  G A+EAL LF  M
Sbjct: 367 HNIYVATGLVDMYGKCGRVEEALRVFEAMPVKNEATWNAMITAYAHQGHAKEALLLFDRM 426

Query: 424 IKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRA 483
                S  P+DITF+GVLSACVHAGLV +G R FH MSS F LVPKIEHY+ ++DLLSRA
Sbjct: 427 -----SVPPSDITFIGVLSACVHAGLVHQGCRYFHEMSSMFGLVPKIEHYTNIIDLLSRA 486

Query: 484 GHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLEL-EPSNSGNYVIS 543
           G L+EAW+F+++ P K DE++L A+LGAC KRK+V I E+ M +L+E+ E  N+GNYVIS
Sbjct: 487 GMLDEAWEFMERFPGKPDEIMLAAILGACHKRKDVAIREKAMRMLMEMKEAKNAGNYVIS 546

Query: 544 SRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAG-DVLHQEWIEIHQI 603
           S + A+++ WD+SA+MR LM+ +GV KTPGCSWI+I  +L EF AG D L     +   +
Sbjct: 547 SNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDSGSL 606

Query: 604 LDLLIDDLRREGY 613
            DLL+++++RE Y
Sbjct: 607 FDLLVEEMKRERY 614

BLAST of Lag0006258.1 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.8e-124
Identity = 240/624 (38.46%), Postives = 366/624 (58.65%), Query Frame = 0

Query: 36  VAFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVE-------LKDFAYASDFFSKILE 95
           ++ L  C T++ L+ IH QM+   +H  N  L K++E        +   YA   F  I E
Sbjct: 37  LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQE 96

Query: 96  PTEYSFNVMIRGLSTTWNKSSLALDFYSRMKLLGLRPNKLTYPFLFIACSNLLAVGSGRV 155
           P    +N M RG + + +  S AL  Y  M  LGL PN  T+PF+  +C+   A   G+ 
Sbjct: 97  PNLLIWNTMFRGHALSSDPVS-ALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 156

Query: 156 GHCSVIKRGLDEDGHVSHSLITMYARCGELGDARKV------------------------ 215
            H  V+K G D D +V  SLI+MY + G L DA KV                        
Sbjct: 157 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 216

Query: 216 -------FDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 275
                  FDEIP +D+VSWN+MISGY++     EA+ LF++MM+    P+E T+V+V+ A
Sbjct: 217 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 276

Query: 276 CGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVTW 335
           C + G ++LG  V  ++ ++    N  + +ALI +Y KCG+L +A  +F+ +  KD ++W
Sbjct: 277 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 336

Query: 336 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 395
           N +I GY    + +EA+ LFQ+M  S  TP+ +T++ +L ACA +GA+D+G+ + +Y  +
Sbjct: 337 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 396

Query: 396 R--GLQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEAL 455
           R  G+ +   + T+L+DMYAKCG ++ A +VF+S+  K+  SWNAMI   A HG+A  + 
Sbjct: 397 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASF 456

Query: 456 ALFKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMV 515
            LF  M K G    P+DITFVG+LSAC H+G++D GR +F  M+  +++ PK+EHY CM+
Sbjct: 457 DLFSRMRKIG--IQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 516

Query: 516 DLLSRAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISERVMNLLLELEPSNSG 575
           DLL  +G  +EA + +  M  + D VI  +LL AC+   NV++ E     L+++EP N G
Sbjct: 517 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 576

Query: 576 NYVISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIE 620
           +YV+ S +YA+  RW++ A+ R L+  KG+ K PGCS I+I+S +HEF  GD  H    E
Sbjct: 577 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 636

BLAST of Lag0006258.1 vs. TAIR 10
Match: AT3G12770.1 (mitochondrial editing factor 22 )

HSP 1 Score: 426.0 bits (1094), Expect = 5.1e-119
Identity = 218/575 (37.91%), Postives = 346/575 (60.17%), Query Frame = 0

Query: 47  QLKQIHTQMLTNSIHKPNSFLYKIVELK----DFAYASDFFSKILEPTEYSFNVMIRGLS 106
           QLKQIH ++L   +      + K++       D  +A   F  +  P  + +N +IRG S
Sbjct: 36  QLKQIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYS 95

Query: 107 TTWNKSSLALDFYSRMKLLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDG 166
              N    AL  YS M+L  + P+  T+P L  ACS L  +  GR  H  V + G D D 
Sbjct: 96  RN-NHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADV 155

Query: 167 HVSHSLITMYARCGELGDARKVFD--EIPERDLVSWNSMISGYSKMRYAGEAVGLFREMM 226
            V + LI +YA+C  LG AR VF+   +PER +VSW +++S Y++     EA+ +F +M 
Sbjct: 156 FVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMR 215

Query: 227 EAGFEPNEMTLVSVLGACGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLA 286
           +   +P+ + LVSVL A   L +LK G  +   V++  + +   +  +L  MY KCG +A
Sbjct: 216 KMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVA 275

Query: 287 SARRIFDSMKKKDKVTWNAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACA 346
           +A+ +FD MK  + + WNAMI+GYA+NG + EAI +F +M      PD I++   +SACA
Sbjct: 276 TAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACA 335

Query: 347 SIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLDNAFRVFDSMTKKNEVSWNA 406
            +G+L+  + +  Y      +DD+++++AL+DM+AKCGS++ A  VFD    ++ V W+A
Sbjct: 336 QVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSA 395

Query: 407 MIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMSS 466
           MI     HG+A+EA++L+++M  E G   PND+TF+G+L AC H+G+V EG   F+ M+ 
Sbjct: 396 MIVGYGLHGRAREAISLYRAM--ERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMAD 455

Query: 467 TFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEVILGALLGACQKRKNVDISE 526
             ++ P+ +HY+C++DLL RAGHL++A++ +K MP +    + GALL AC+K ++V++ E
Sbjct: 456 -HKINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGE 515

Query: 527 RVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQL 586
                L  ++PSN+G+YV  S LYA  R WD  A +R+ MK+KG++K  GCSW+++  +L
Sbjct: 516 YAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRL 575

Query: 587 HEFHAGDVLHQEWIEIHQILDLLIDDLRREGYVPN 616
             F  GD  H  + EI + ++ +   L+  G+V N
Sbjct: 576 EAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVAN 606

BLAST of Lag0006258.1 vs. TAIR 10
Match: AT2G29760.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 424.1 bits (1089), Expect = 1.9e-118
Identity = 228/652 (34.97%), Postives = 372/652 (57.06%), Query Frame = 0

Query: 6   ASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQML-TNSIHKPN 65
           A P  + R+ NF   +  +  N R+   + ++ +++C++++QLKQ H  M+ T +   P 
Sbjct: 7   AQPLSLPRHPNFSNPNQPTTNNERS---RHISLIERCVSLRQLKQTHGHMIRTGTFSDPY 66

Query: 66  SF--LYKIVELKDFA---YASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALDFYSRMK 125
           S   L+ +  L  FA   YA   F +I +P  +++N +IR  ++  +       F   + 
Sbjct: 67  SASKLFAMAALSSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDMVS 126

Query: 126 LLGLRPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMYARCGELG 185
                PNK T+PFL  A + + ++  G+  H   +K  +  D  V++SLI  Y  CG+L 
Sbjct: 127 ESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLD 186

Query: 186 DARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACG 245
            A KVF  I E+D+VSWNSMI+G+ +     +A+ LF++M     + + +T+V VL AC 
Sbjct: 187 SACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACA 246

Query: 246 ELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKKDKVT--- 305
           ++  L+ G  V  ++ EN++ +N  + +A++ MY KCG +  A+R+FD+M++KD VT   
Sbjct: 247 KIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTT 306

Query: 306 ----------------------------WNAMITGYAQNGMSEEAIKLFQDMRMSSTTP- 365
                                       WNA+I+ Y QNG   EA+ +F ++++      
Sbjct: 307 MLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNMKL 366

Query: 366 DQITLIGMLSACASIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLDNAFRVF 425
           +QITL+  LSACA +GAL+LG+ +  Y  + G++ + +V +AL+ MY+KCG L+ +  VF
Sbjct: 367 NQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSREVF 426

Query: 426 DSMTKKNEVSWNAMIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSACVHAGL 485
           +S+ K++   W+AMI  LA HG   EA+ +F  M  +  +  PN +TF  V  AC H GL
Sbjct: 427 NSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKM--QEANVKPNGVTFTNVFCACSHTGL 486

Query: 486 VDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEVILGALL 545
           VDE   LFH M S + +VP+ +HY+C+VD+L R+G+LE+A  F++ MP      + GALL
Sbjct: 487 VDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALL 546

Query: 546 GACQKRKNVDISERVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMKQKGVSK 605
           GAC+   N++++E     LLELEP N G +V+ S +YA L +W++ + +R  M+  G+ K
Sbjct: 547 GACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKK 606

Query: 606 TPGCSWIDINSQLHEFHAGDVLHQEWIEIHQILDLLIDDLRREGYVPNTSLV 620
            PGCS I+I+  +HEF +GD  H    +++  L  +++ L+  GY P  S V
Sbjct: 607 EPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEISQV 653

BLAST of Lag0006258.1 vs. TAIR 10
Match: AT2G22410.1 (SLOW GROWTH 1 )

HSP 1 Score: 422.9 bits (1086), Expect = 4.3e-118
Identity = 241/642 (37.54%), Postives = 367/642 (57.17%), Query Frame = 0

Query: 3   RIKASPHLISRYLNFDKAHSNSNPNRRALSQKLVAFLQQCLTIKQLKQIHTQMLTNSI-- 62
           R ++ PH   + +N++  HS        L   L++ L++C  +  LKQI  QM+ N +  
Sbjct: 30  RTRSLPHHRDKPINWNSTHS------FVLHNPLLSLLEKCKLLLHLKQIQAQMIINGLIL 89

Query: 63  -HKPNSFLYKIVELKDFAYASDFFSKILE----PTEYSFNVMIRGLSTTWNKSSLALDFY 122
               +S L     L +  Y  D+  KIL+    P  +S+NV IRG S + N     L  Y
Sbjct: 90  DPFASSRLIAFCALSESRYL-DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFL-LY 149

Query: 123 SRMKLLGL---RPNKLTYPFLFIACSNLLAVGSGRVGHCSVIKRGLDEDGHVSHSLITMY 182
            +M   G    RP+  TYP LF  C++L     G +    V+K  L+   HV ++ I M+
Sbjct: 150 KQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGHMILGHVLKLRLELVSHVHNASIHMF 209

Query: 183 ARCGELGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLV 242
           A CG++ +ARKVFDE P RDLVSWN +I+GY K+  A +A+ +++ M   G +P+++T++
Sbjct: 210 ASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDDVTMI 269

Query: 243 SVLGACGELGELKLGTWVEEFVMENKMVLNSYMGSALIHMYGKCGDLASARRIFDSMKKK 302
            ++ +C  LG+L  G    E+V EN + +   + +AL+ M+ KCGD+  ARRIFD+++K+
Sbjct: 270 GLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDNLEKR 329

Query: 303 DKVTWNAMITGYAQNGM-------------------------------SEEAIKLFQDMR 362
             V+W  MI+GYA+ G+                                ++A+ LFQ+M+
Sbjct: 330 TIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALFQEMQ 389

Query: 363 MSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLQDDIYVATALVDMYAKCGSLD 422
            S+T PD+IT+I  LSAC+ +GALD+G  +  Y  +  L  ++ + T+LVDMYAKCG++ 
Sbjct: 390 TSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSLVDMYAKCGNIS 449

Query: 423 NAFRVFDSMTKKNEVSWNAMIAALAFHGQAQEALALFKSMIKEGGSASPNDITFVGVLSA 482
            A  VF  +  +N +++ A+I  LA HG A  A++ F  MI  G   +P++ITF+G+LSA
Sbjct: 450 EALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAG--IAPDEITFIGLLSA 509

Query: 483 CVHAGLVDEGRRLFHMMSSTFRLVPKIEHYSCMVDLLSRAGHLEEAWDFVKKMPEKADEV 542
           C H G++  GR  F  M S F L P+++HYS MVDLL RAG LEEA   ++ MP +AD  
Sbjct: 510 CCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPMEADAA 569

Query: 543 ILGALLGACQKRKNVDISERVMNLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMLMK 602
           + GALL  C+   NV++ E+    LLEL+PS+SG YV+   +Y     W+D+ R R +M 
Sbjct: 570 VWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANMWEDAKRARRMMN 629

Query: 603 QKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQILDLL 604
           ++GV K PGCS I++N  + EF   D    E  +I+  L  L
Sbjct: 630 ERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCL 661

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023521362.10.0e+0091.28pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp... [more]
XP_022929625.10.0e+0090.95pentatricopeptide repeat-containing protein At2g34400 [Cucurbita moschata][more]
XP_022997564.10.0e+0090.63pentatricopeptide repeat-containing protein At2g34400 [Cucurbita maxima][more]
XP_038889591.10.0e+0090.79pentatricopeptide repeat-containing protein At2g34400 [Benincasa hispida][more]
KAG6598886.10.0e+0090.31Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
O647053.0e-18854.81Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX... [more]
Q9LN012.6e-12338.46Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop... [more]
Q9LTV87.2e-11837.91Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX... [more]
O823802.7e-11734.97Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Q9SJZ36.1e-11737.54Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1EUW30.0e+0090.95pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita moschata OX=3... [more]
A0A6J1KBU40.0e+0090.63pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita maxima OX=366... [more]
A0A6J1DIG60.0e+0090.98pentatricopeptide repeat-containing protein At2g34400 OS=Momordica charantia OX=... [more]
A0A0A0LMG00.0e+0088.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060420 PE=4 SV=1[more]
A0A1S3BRJ20.0e+0087.88pentatricopeptide repeat-containing protein At2g34400 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT2G34400.12.1e-18954.81Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G08070.11.8e-12438.46Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G12770.15.1e-11937.91mitochondrial editing factor 22 [more]
AT2G29760.11.9e-11834.97Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G22410.14.3e-11837.54SLOW GROWTH 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 470..497
e-value: 9.4E-4
score: 19.3
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 471..496
e-value: 3.0E-4
score: 18.8
coord: 194..228
e-value: 1.0E-9
score: 36.0
coord: 396..425
e-value: 2.0E-4
score: 19.3
coord: 295..328
e-value: 3.5E-9
score: 34.3
coord: 368..394
e-value: 0.0026
score: 15.8
coord: 433..460
e-value: 0.0013
score: 16.8
coord: 268..292
e-value: 0.001
score: 17.1
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 292..340
e-value: 5.3E-13
score: 49.0
coord: 393..443
e-value: 3.7E-9
score: 36.7
coord: 192..238
e-value: 7.6E-10
score: 38.8
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 192..226
score: 13.153618
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 467..501
score: 8.801982
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 90..125
score: 9.29524
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 262..292
score: 8.845827
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 363..393
score: 9.076014
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 394..428
score: 10.742131
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 293..327
score: 12.956314
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 151..248
e-value: 1.0E-22
score: 82.4
coord: 249..351
e-value: 9.9E-26
score: 92.2
coord: 12..142
e-value: 3.7E-7
score: 31.6
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 369..592
e-value: 1.1E-41
score: 145.2
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 262..422
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 190..289
coord: 48..189
NoneNo IPR availablePANTHERPTHR47926:SF54SUBFAMILY NOT NAMEDcoord: 290..617
coord: 190..289
NoneNo IPR availablePANTHERPTHR47926:SF54SUBFAMILY NOT NAMEDcoord: 48..189
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 290..617

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0006258Lag0006258gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0006258.1.exon2Lag0006258.1.exon2exon
Lag0006258.1.exon1Lag0006258.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0006258.1cds.Lag0006258.1_2CDS
cds.Lag0006258.1cds.Lag0006258.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0006258.1Lag0006258.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding