Lag0002401.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0002401.1 vs. NCBI nr
Match: XP_038896874.1 (protein TIME FOR COFFEE-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1998.4 bits (5176), Expect = 0.0e+00
Identity = 1108/1298 (85.36%), Postives = 1156/1298 (89.06%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRIL-SSTTASSAS 120
            FASRSKRRRGGGNREEEVEE EGGDTSAEDIVADG S EVED GGVSRIL SSTTASS S
Sbjct: 61   FASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADGDSYEVEDGGGVSRILSSSTTASSVS 120

Query: 121  NQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGEDI 180
            NQNQ+RNSLPPRVAKQQWK VDDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGEDI
Sbjct: 121  NQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGEDI 180

Query: 181  ADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDIE 240
            AD+HSHRN SDSPARSSAEVVSPSTSIISA KKMK TGPKTRSMKTSK SS SAKEGDIE
Sbjct: 181  ADDHSHRNQSDSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGDIE 240

Query: 241  IEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAFQK 300
            IEIAEVLFGLKKQPQCSKKQEVNVK SS QETENSSVLRD +KSSV S TANSS TAFQK
Sbjct: 241  IEIAEVLFGLKKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAFQK 300

Query: 301  SVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKLER 360
            S SLQKN AISDL LNVA EK KV+SS       SLDG AKGESE+PA+ +EIYP KLE 
Sbjct: 301  SASLQKNCAISDLSLNVAGEKKKVDSS-------SLDGTAKGESEKPAE-VEIYPSKLEV 360

Query: 361  ASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSPPT 420
            AS             EESKP   V TGGDENKGS++T L  ED  SCAKGDVD EDS  T
Sbjct: 361  AS-------------EESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLRT 420

Query: 421  KSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEVVG 480
            +SIP    QKEEKFKFDLMAPPTSPERDGL DMV DAKPLSLGIEMGKETSNKVENEV G
Sbjct: 421  RSIPEAVNQKEEKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVEG 480

Query: 481  FKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKNES 540
            FKEKEKV+DEDK+VTSG   EFFKLDLEKPQLD N I MQEQSQKQQ KGAA TVEKNE 
Sbjct: 481  FKEKEKVIDEDKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEKNEP 540

Query: 541  STSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRCLT 600
            STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQHPNFILS+PRPKRCLT
Sbjct: 541  STSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKRCLT 600

Query: 601  HYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLN 660
            HYDVARNIYLHQQFTKT+Y HP GDASASLAE KLKNMSSKEGM+LSN L GNHL+V+LN
Sbjct: 601  HYDVARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDVNLN 660

Query: 661  SVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPI 720
            SVQQR+Q EGDF GNVVNDKSPEAANFAD+AKSKQLVFQQR GA FGNSM PGSGFIFPI
Sbjct: 661  SVQQREQAEGDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSMQPGSGFIFPI 720

Query: 721  GQHQAP-TAATGNQSGSTKS-SNQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSI 780
            GQHQAP  AAT NQSGS KS +NQSTSLFNNPEAGTLVSFPA P VS+NMSYSHPNVVS+
Sbjct: 721  GQHQAPIAAATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNVVSV 780

Query: 781  EAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQS 840
            EAPYLAKLQ+ GYPFTFSTPAGTSATYRA+NAQPLPLFNGSFYPSQLFHPSQIQPAQTQ+
Sbjct: 781  EAPYLAKLQSNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQTQT 840

Query: 841  QHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETSGE 900
             HQPSGSHKQPQ+QQQQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETSGE
Sbjct: 841  HHQPSGSHKQPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETSGE 900

Query: 901  DATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNLKS 960
            D TSL DKRAA VQKNAYGQN+ILPVQALGFTLMPSATMNNGN G +G+KKQSH+QNLKS
Sbjct: 901  DTTSLSDKRAASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNLKS 960

Query: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQ 1020
            TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNT V+QNLPD+QR GFQVAP PQATKQ
Sbjct: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQATKQ 1020

Query: 1021 KNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNWPS 1080
            KNHQTSEGRNGV++SG +DRNTGNSS KPS ATSGQTLVFGNS+RTLNFMTSSVPVNWPS
Sbjct: 1021 KNHQTSEGRNGVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNWPS 1080

Query: 1081 PSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQQQQATMAPRVAPRTKVPTNNTLPS 1140
             STKSAASTTNRPAGSSSG+               QQQQATMA R+APRTKVPTNNTLPS
Sbjct: 1081 HSTKSAASTTNRPAGSSSGN---------------QQQQATMASRMAPRTKVPTNNTLPS 1140

Query: 1141 SATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHLASSTAVHKNSTQ 1200
            SATTKFP NAPPGFSQPLIQCDNSIQSPAQKNSGR+VAS VPMTSLHLASS AVHKNS Q
Sbjct: 1141 SATTKFPCNAPPGFSQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQ 1200

Query: 1201 QKGSTPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNT-SSKTSPSVAPAQPK 1260
            QKGS  QGQTQISF GGDFKPA+TQMQHIPTS HSPSSSGKLRN  SSKTSPSV P QPK
Sbjct: 1201 QKGSNTQGQTQISFGGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPK 1259

Query: 1261 QDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            QDE SS+TGAGQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 QDE-SSSTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1259

BLAST of Lag0002401.1 vs. NCBI nr
Match: XP_038896875.1 (protein TIME FOR COFFEE-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1994.5 bits (5166), Expect = 0.0e+00
Identity = 1108/1298 (85.36%), Postives = 1156/1298 (89.06%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRIL-SSTTASSAS 120
            FASRSKRRRGGGNREEEVEE EGGDTSAEDIVADG S EVED GGVSRIL SSTTASS S
Sbjct: 61   FASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADGDSYEVEDGGGVSRILSSSTTASSVS 120

Query: 121  NQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGEDI 180
            NQNQ+RNSLPPRVAKQQWK VDDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGEDI
Sbjct: 121  NQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGEDI 180

Query: 181  ADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDIE 240
            AD+HSHRN SDSPARSSAEVVSPSTSIISA KKMK TGPKTRSMKTSK SS SAKEGDIE
Sbjct: 181  ADDHSHRNQSDSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGDIE 240

Query: 241  IEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAFQK 300
            IEIAEVLFGLKKQPQCSKKQEVNVK SS QETENSSVLRD +KSSV S TANSS TAFQK
Sbjct: 241  IEIAEVLFGLKKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAFQK 300

Query: 301  SVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKLER 360
            S SLQKN AISDL LNVA EK KV+SS       SLDG AKGESE+PA+ +EIYP KLE 
Sbjct: 301  SASLQKNCAISDLSLNVAGEKKKVDSS-------SLDGTAKGESEKPAE-VEIYPSKLEV 360

Query: 361  ASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSPPT 420
            AS             EESKP   V TGGDENKGS++T L  ED  SCAKGDVD EDS  T
Sbjct: 361  AS-------------EESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLRT 420

Query: 421  KSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEVVG 480
            +SIP    QKEEKFKFDLMAPPTSPERDGL DMV DAKPLSLGIEMGKETSNKVENEV G
Sbjct: 421  RSIPEAVNQKEEKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVEG 480

Query: 481  FKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKNES 540
            FKEKEKV+DEDK+VTSG   EFFKLDLEKPQLD N I MQEQSQKQQ KGAA TVEKNE 
Sbjct: 481  FKEKEKVIDEDKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEKNEP 540

Query: 541  STSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRCLT 600
            STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQHPNFILS+PRPKRCLT
Sbjct: 541  STSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKRCLT 600

Query: 601  HYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLN 660
            HYDVARNIYLHQQFTKT+Y HP GDASASLAE KLKNMSSKEGM+LSN L GNHL+V+LN
Sbjct: 601  HYDVARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDVNLN 660

Query: 661  SVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPI 720
            SVQQR+Q EGDF GNVVNDKSPEAANFAD+AKSKQLVFQQR GA FGNSM PGSGFIFPI
Sbjct: 661  SVQQREQAEGDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSM-PGSGFIFPI 720

Query: 721  GQHQAP-TAATGNQSGSTKS-SNQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSI 780
            GQHQAP  AAT NQSGS KS +NQSTSLFNNPEAGTLVSFPA P VS+NMSYSHPNVVS+
Sbjct: 721  GQHQAPIAAATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNVVSV 780

Query: 781  EAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQS 840
            EAPYLAKLQ+ GYPFTFSTPAGTSATYRA+NAQPLPLFNGSFYPSQLFHPSQIQPAQTQ+
Sbjct: 781  EAPYLAKLQSNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQTQT 840

Query: 841  QHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETSGE 900
             HQPSGSHKQPQ+QQQQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETSGE
Sbjct: 841  HHQPSGSHKQPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETSGE 900

Query: 901  DATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNLKS 960
            D TSL DKRAA VQKNAYGQN+ILPVQALGFTLMPSATMNNGN G +G+KKQSH+QNLKS
Sbjct: 901  DTTSLSDKRAASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNLKS 960

Query: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQ 1020
            TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNT V+QNLPD+QR GFQVAP PQATKQ
Sbjct: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQATKQ 1020

Query: 1021 KNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNWPS 1080
            KNHQTSEGRNGV++SG +DRNTGNSS KPS ATSGQTLVFGNS+RTLNFMTSSVPVNWPS
Sbjct: 1021 KNHQTSEGRNGVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNWPS 1080

Query: 1081 PSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQQQQATMAPRVAPRTKVPTNNTLPS 1140
             STKSAASTTNRPAGSSSG+               QQQQATMA R+APRTKVPTNNTLPS
Sbjct: 1081 HSTKSAASTTNRPAGSSSGN---------------QQQQATMASRMAPRTKVPTNNTLPS 1140

Query: 1141 SATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHLASSTAVHKNSTQ 1200
            SATTKFP NAPPGFSQPLIQCDNSIQSPAQKNSGR+VAS VPMTSLHLASS AVHKNS Q
Sbjct: 1141 SATTKFPCNAPPGFSQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQ 1200

Query: 1201 QKGSTPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNT-SSKTSPSVAPAQPK 1260
            QKGS  QGQTQISF GGDFKPA+TQMQHIPTS HSPSSSGKLRN  SSKTSPSV P QPK
Sbjct: 1201 QKGSNTQGQTQISFGGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPK 1258

Query: 1261 QDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            QDE SS+TGAGQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 QDE-SSSTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1258

BLAST of Lag0002401.1 vs. NCBI nr
Match: XP_004147004.1 (protein TIME FOR COFFEE isoform X1 [Cucumis sativus] >KGN44812.1 hypothetical protein Csa_016126 [Cucumis sativus])

HSP 1 Score: 1966.8 bits (5094), Expect = 0.0e+00
Identity = 1089/1303 (83.58%), Postives = 1153/1303 (88.49%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVED-VGGVSRILSS-TTASSA 120
            F SRSKRRR GGNREEE+EE+EGGDTSAEDIVADG  DEVED  GGVSRILSS TTASS 
Sbjct: 61   FVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADGDIDEVEDGGGGVSRILSSTTTASSV 120

Query: 121  SN--QNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAG 180
            SN  QNQ+R SLPPRV KQQWK  DDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAG
Sbjct: 121  SNQHQNQKRTSLPPRVVKQQWKVADDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAG 180

Query: 181  EDIADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEG 240
            EDIAD+HSHRN +DSPARSSAEVVSPSTSIISA KKMKPTGPKTRSMKTS  SS SAKEG
Sbjct: 181  EDIADDHSHRNQTDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEG 240

Query: 241  DIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTA 300
            DIEIEIAEVLFGLKKQP CSKKQEV  K SS QETENSSVLRD +KSSV S  ANS+QTA
Sbjct: 241  DIEIEIAEVLFGLKKQPHCSKKQEVITKQSSKQETENSSVLRDGSKSSVTSTMANSAQTA 300

Query: 301  FQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQK 360
            F KSVSLQKN  ISDL LNVA EK KV+SS       +LD A KGESE+PA+ + IYP K
Sbjct: 301  FNKSVSLQKNDVISDLSLNVAGEKQKVDSS-------TLDFAGKGESEKPAE-IAIYPSK 360

Query: 361  LERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDS 420
            LE AS             EESKP   ++TGGDENKGS+KT L QED+ SC KGDVD EDS
Sbjct: 361  LEGAS-------------EESKPAKEIFTGGDENKGSKKTGLAQEDITSCLKGDVDPEDS 420

Query: 421  PPTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENE 480
            PP  SIP   TQKEEKFKFDLMAPPTSPERDGLADMVLD KPLSLGIEMGKETSNKVENE
Sbjct: 421  PPNNSIPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSNKVENE 480

Query: 481  VVGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEK 540
            V GFKEKEKV++EDKMVTSG   EFFKLDLEKPQLD N I M+EQSQKQQ KGAA TVEK
Sbjct: 481  VEGFKEKEKVINEDKMVTSGTKFEFFKLDLEKPQLDSNNITMKEQSQKQQPKGAASTVEK 540

Query: 541  NESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKR 600
            NE STSVRLPIILGGWPTTEIP VGY+PPFRTVLPVDS+DK STKLQ+ NFILSHPRPKR
Sbjct: 541  NEPSTSVRLPIILGGWPTTEIPSVGYLPPFRTVLPVDSIDKSSTKLQNLNFILSHPRPKR 600

Query: 601  CLTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEV 660
            CLTHYD+ARNIYLHQQFTKT+Y HP GDASASL E KLKN+SSKEGM+LSN L GNHL++
Sbjct: 601  CLTHYDIARNIYLHQQFTKTNYFHPAGDASASLVEAKLKNISSKEGMLLSNQLSGNHLDM 660

Query: 661  SLNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFI 720
            +LNSVQQR+Q EGD  GNVVNDKS EAANFAD+AKSKQLVF Q+QG   GNSM P SGFI
Sbjct: 661  NLNSVQQREQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPLGNSM-PSSGFI 720

Query: 721  FPIGQHQAPTA-ATGNQSGSTKSS-NQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNV 780
            FPIGQHQAP A AT NQSGS KSS NQSTSLF+NPEAGTLVSFPA PAVS+NMSYSHPNV
Sbjct: 721  FPIGQHQAPIAQATANQSGSAKSSNNQSTSLFSNPEAGTLVSFPAFPAVSTNMSYSHPNV 780

Query: 781  VSIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQ 840
            VS+EAPYLAKLQN GYPFTFSTPAGTSATYR NNAQPLPLFNGSFYPSQLFHPSQIQPAQ
Sbjct: 781  VSVEAPYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYPSQLFHPSQIQPAQ 840

Query: 841  TQSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESET 900
            TQS HQPSGSHKQPQ+Q QQW VHVP NN++PS+GMQ+KQSTEQ+LP+SNH SRKHE+ET
Sbjct: 841  TQSHHQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQLKQSTEQHLPLSNH-SRKHENET 900

Query: 901  SGEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQN 960
            SGED TSL DKRAAPVQKNAYGQN+ILPVQALGFTLMPS T+NNGN+GN G+KKQSHTQN
Sbjct: 901  SGEDTTSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHTQN 960

Query: 961  LKSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQA 1020
            LK+TVALVPSQGLTMSFASYNGNGTPSNLNFTPI+QNT V+QNLPD+QR GFQVAP PQA
Sbjct: 961  LKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPISQNTTVYQNLPDIQRTGFQVAPVPQA 1020

Query: 1021 TKQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVN 1080
            TKQKNHQTSEGRNGVV+SG +DRNTGNSS KPS ATSGQTLVFGN +RTLNFMTSSVPVN
Sbjct: 1021 TKQKNHQTSEGRNGVVLSGASDRNTGNSSTKPSTATSGQTLVFGNPSRTLNFMTSSVPVN 1080

Query: 1081 WPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLP-QQHIMQQQQATMAPRVAPRTKVPTNN 1140
            WPSPS KSAA TTNRPAGSSSG+QQQQPLLQLP QQHI+QQQQA M PR+APRTKVPTNN
Sbjct: 1081 WPSPSNKSAA-TTNRPAGSSSGNQQQQPLLQLPQQQHILQQQQAAMGPRMAPRTKVPTNN 1140

Query: 1141 TLPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHL-ASSTAVH 1200
            TLPSSATTKFP N PPGFSQPLIQCDNSIQSPAQKNSGRM AS VPMTSLHL +SST VH
Sbjct: 1141 TLPSSATTKFPCNPPPGFSQPLIQCDNSIQSPAQKNSGRMTASVVPMTSLHLSSSSTPVH 1200

Query: 1201 KNSTQQKG-STPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVA 1260
            K+S QQKG STPQGQTQISF GGDFKPA+T MQHIPTS HSPSSSGKLRNT+SKT+PSV 
Sbjct: 1201 KSSIQQKGSSTPQGQTQISFGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVT 1260

Query: 1261 PAQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            P Q KQDE SS+TGAGQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 PTQQKQDE-SSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1278

BLAST of Lag0002401.1 vs. NCBI nr
Match: XP_038896876.1 (protein TIME FOR COFFEE-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1965.7 bits (5091), Expect = 0.0e+00
Identity = 1096/1298 (84.44%), Postives = 1144/1298 (88.14%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRIL-SSTTASSAS 120
            FASRSKRRRGGGNREEEVEE EGGDTSAEDIVADG S EVED GGVSRIL SSTTASS S
Sbjct: 61   FASRSKRRRGGGNREEEVEE-EGGDTSAEDIVADGDSYEVEDGGGVSRILSSSTTASSVS 120

Query: 121  NQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGEDI 180
            NQNQ+RNSLPPRVAKQQWK VDDAMIG PVPRKAR            SGNSGVGGAGEDI
Sbjct: 121  NQNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKAR------------SGNSGVGGAGEDI 180

Query: 181  ADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDIE 240
            AD+HSHRN SDSPARSSAEVVSPSTSIISA KKMK TGPKTRSMKTSK SS SAKEGDIE
Sbjct: 181  ADDHSHRNQSDSPARSSAEVVSPSTSIISAKKKMKSTGPKTRSMKTSKDSSRSAKEGDIE 240

Query: 241  IEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAFQK 300
            IEIAEVLFGLKKQPQCSKKQEVNVK SS QETENSSVLRD +KSSV S TANSS TAFQK
Sbjct: 241  IEIAEVLFGLKKQPQCSKKQEVNVKQSSKQETENSSVLRDASKSSVTSTTANSSPTAFQK 300

Query: 301  SVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKLER 360
            S SLQKN AISDL LNVA EK KV+SS       SLDG AKGESE+PA+ +EIYP KLE 
Sbjct: 301  SASLQKNCAISDLSLNVAGEKKKVDSS-------SLDGTAKGESEKPAE-VEIYPSKLEV 360

Query: 361  ASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSPPT 420
            AS             EESKP   V TGGDENKGS++T L  ED  SCAKGDVD EDS  T
Sbjct: 361  AS-------------EESKPAKEVCTGGDENKGSKRTGLAPEDANSCAKGDVDPEDSLRT 420

Query: 421  KSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEVVG 480
            +SIP    QKEEKFKFDLMAPPTSPERDGL DMV DAKPLSLGIEMGKETSNKVENEV G
Sbjct: 421  RSIPEAVNQKEEKFKFDLMAPPTSPERDGLTDMVPDAKPLSLGIEMGKETSNKVENEVEG 480

Query: 481  FKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKNES 540
            FKEKEKV+DEDK+VTSG   EFFKLDLEKPQLD N I MQEQSQKQQ KGAA TVEKNE 
Sbjct: 481  FKEKEKVIDEDKIVTSGTKFEFFKLDLEKPQLDSNSIAMQEQSQKQQPKGAASTVEKNEP 540

Query: 541  STSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRCLT 600
            STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQHPNFILS+PRPKRCLT
Sbjct: 541  STSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHPNFILSYPRPKRCLT 600

Query: 601  HYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLN 660
            HYDVARNIYLHQQFTKT+Y HP GDASASLAE KLKNMSSKEGM+LSN L GNHL+V+LN
Sbjct: 601  HYDVARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNMSSKEGMLLSNPLTGNHLDVNLN 660

Query: 661  SVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPI 720
            SVQQR+Q EGDF GNVVNDKSPEAANFAD+AKSKQLVFQQR GA FGNSM PGSGFIFPI
Sbjct: 661  SVQQREQAEGDFPGNVVNDKSPEAANFADIAKSKQLVFQQRPGAPFGNSMQPGSGFIFPI 720

Query: 721  GQHQAP-TAATGNQSGSTKS-SNQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSI 780
            GQHQAP  AAT NQSGS KS +NQSTSLFNNPEAGTLVSFPA P VS+NMSYSHPNVVS+
Sbjct: 721  GQHQAPIAAATANQSGSAKSPNNQSTSLFNNPEAGTLVSFPAFPPVSTNMSYSHPNVVSV 780

Query: 781  EAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQS 840
            EAPYLAKLQ+ GYPFTFSTPAGTSATYRA+NAQPLPLFNGSFYPSQLFHPSQIQPAQTQ+
Sbjct: 781  EAPYLAKLQSNGYPFTFSTPAGTSATYRASNAQPLPLFNGSFYPSQLFHPSQIQPAQTQT 840

Query: 841  QHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETSGE 900
             HQPSGSHKQPQ+QQQQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETSGE
Sbjct: 841  HHQPSGSHKQPQTQQQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETSGE 900

Query: 901  DATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNLKS 960
            D TSL DKRAA VQKNAYGQN+ILPVQALGFTLMPSATMNNGN G +G+KKQSH+QNLKS
Sbjct: 901  DTTSLSDKRAASVQKNAYGQNYILPVQALGFTLMPSATMNNGNGGYHGEKKQSHSQNLKS 960

Query: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQ 1020
            TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNT V+QNLPD+QR GFQVAP PQATKQ
Sbjct: 961  TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTTVYQNLPDIQRTGFQVAPTPQATKQ 1020

Query: 1021 KNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNWPS 1080
            KNHQTSEGRNGV++SG +DRNTGNSS KPS ATSGQTLVFGNS+RTLNFMTSSVPVNWPS
Sbjct: 1021 KNHQTSEGRNGVILSGTSDRNTGNSSTKPSTATSGQTLVFGNSSRTLNFMTSSVPVNWPS 1080

Query: 1081 PSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQQQQATMAPRVAPRTKVPTNNTLPS 1140
             STKSAASTTNRPAGSSSG+               QQQQATMA R+APRTKVPTNNTLPS
Sbjct: 1081 HSTKSAASTTNRPAGSSSGN---------------QQQQATMASRMAPRTKVPTNNTLPS 1140

Query: 1141 SATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHLASSTAVHKNSTQ 1200
            SATTKFP NAPPGFSQPLIQCDNSIQSPAQKNSGR+VAS VPMTSLHLASS AVHKNS Q
Sbjct: 1141 SATTKFPCNAPPGFSQPLIQCDNSIQSPAQKNSGRLVASVVPMTSLHLASSAAVHKNSIQ 1200

Query: 1201 QKGSTPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNT-SSKTSPSVAPAQPK 1260
            QKGS  QGQTQISF GGDFKPA+TQMQHIPTS HSPSSSGKLRN  SSKTSPSV P QPK
Sbjct: 1201 QKGSNTQGQTQISFGGGDFKPAYTQMQHIPTSGHSPSSSGKLRNNISSKTSPSVTPTQPK 1247

Query: 1261 QDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            QDE SS+TGAGQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 QDE-SSSTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1247

BLAST of Lag0002401.1 vs. NCBI nr
Match: KAA0064078.1 (protein TIME FOR COFFEE isoform X1 [Cucumis melo var. makuwa] >TYK26237.1 protein TIME FOR COFFEE isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1958.7 bits (5073), Expect = 0.0e+00
Identity = 1087/1302 (83.49%), Postives = 1152/1302 (88.48%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKR+RDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRERDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVG-GVSRILSS-TTASSA 120
            F SRSKRRR GGNREEE+EE+EGGDTSAEDIVADG SD VED G GVSRILSS TTASS 
Sbjct: 61   FVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADGDSDGVEDGGVGVSRILSSTTTASSV 120

Query: 121  SNQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180
            SNQNQ+RNSLPPRV KQQWK  DDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGED
Sbjct: 121  SNQNQKRNSLPPRVVKQQWKVADDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180

Query: 181  IADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDI 240
            IAD+HSHRN +DSPARSSAEVVSPSTSIISA KKMKPTGPKTRSMKTS  SS SAKEGDI
Sbjct: 181  IADDHSHRNQTDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEGDI 240

Query: 241  EIEIAEVLFGLKKQPQ-CSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAF 300
            EIEIAEVLFGLKKQPQ CSKKQEVN+K SS QETENSSVLRD +KSS+ S  ANS+QTAF
Sbjct: 241  EIEIAEVLFGLKKQPQHCSKKQEVNMKQSSKQETENSSVLRDGSKSSITSTMANSAQTAF 300

Query: 301  QKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKL 360
             KSVSLQKN  ISDL LNVA EK KV+S         LD A KGESE+PA+ + IYP KL
Sbjct: 301  NKSVSLQKNDVISDLSLNVAGEKQKVDSL-------PLDFAVKGESEKPAE-IAIYPSKL 360

Query: 361  ERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSP 420
            E AS             EESKP   V+TGGDENKGS++T L QED+ SCAKGDVD EDSP
Sbjct: 361  EGAS-------------EESKPAKEVFTGGDENKGSKRTGLAQEDITSCAKGDVDPEDSP 420

Query: 421  PTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEV 480
            P KSIP   TQKEEKFKFDLMAPPTSPERDGLADMVLD KPLSLGIEMGKETS+KVENEV
Sbjct: 421  PNKSIPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSSKVENEV 480

Query: 481  VGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKN 540
             G KEKEKV++EDK+VTSG   EFFKLDLEKPQLD N I MQEQS KQQ KGAA TVEKN
Sbjct: 481  EGLKEKEKVINEDKIVTSGAKFEFFKLDLEKPQLDSNNITMQEQSPKQQPKGAASTVEKN 540

Query: 541  ESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRC 600
            E STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQH NFILSHPRPKRC
Sbjct: 541  EPSTSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHLNFILSHPRPKRC 600

Query: 601  LTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVS 660
            LTHYD+ARNIYLHQQFTKT+Y HP GDASASLAE KLKN+SSKEG +LSN L GNHL+V+
Sbjct: 601  LTHYDIARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNISSKEGTLLSNPLSGNHLDVN 660

Query: 661  LNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIF 720
            LNSV+Q++Q EGD  GNVVNDKS EAANFAD+AKSKQLVF Q+QG  FGNSM P SGFIF
Sbjct: 661  LNSVRQKEQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPFGNSM-PSSGFIF 720

Query: 721  PIGQHQAPTA-ATGNQSGSTKSS-NQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVV 780
            PIGQHQA  A AT NQSGS KSS NQSTSLF+NPE GTLVSFPA PAVS+NMSYSHPNVV
Sbjct: 721  PIGQHQASVAQATANQSGSAKSSNNQSTSLFSNPETGTLVSFPAFPAVSTNMSYSHPNVV 780

Query: 781  SIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQT 840
            S+EAPYLAKLQN GYPFTFSTPAGTSATYR NNAQPLPLFNGSFY SQ+FHPSQIQPAQT
Sbjct: 781  SVEAPYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYSSQMFHPSQIQPAQT 840

Query: 841  QSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETS 900
            QS HQPSGSHKQPQ+Q QQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETS
Sbjct: 841  QSHHQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETS 900

Query: 901  GEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNL 960
            GED TSL DKRAAPVQKNAYGQN+ILPVQALGFTLMPS T+NNGN+GN G+KKQSH QNL
Sbjct: 901  GEDTTSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHPQNL 960

Query: 961  KSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQAT 1020
            K+TVALVPSQGLTMSFASYNGNGTPSNLNF+PI+QNT V+QNLPD+QR GFQVAP PQAT
Sbjct: 961  KNTVALVPSQGLTMSFASYNGNGTPSNLNFSPISQNTTVYQNLPDIQRTGFQVAPVPQAT 1020

Query: 1021 KQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNW 1080
            KQKNHQTSEGRNGVV+SG +DRNTGNSS KPS ATSGQTLVFGN +RTLNFMTSSVPVNW
Sbjct: 1021 KQKNHQTSEGRNGVVLSGASDRNTGNSSTKPSAATSGQTLVFGNPSRTLNFMTSSVPVNW 1080

Query: 1081 PSPSTKSAASTTNRPAGSSSGHQQQQPLLQLP-QQHIMQQQQATMAPRVAPRTKVPTNNT 1140
            PSPSTKSAA TTNRPAGSSSG+QQQQPL QLP QQHI+QQQQA MAPR+APRTKVPTNNT
Sbjct: 1081 PSPSTKSAA-TTNRPAGSSSGNQQQQPLPQLPQQQHILQQQQAAMAPRMAPRTKVPTNNT 1140

Query: 1141 LPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHL-ASSTAVHK 1200
            LPSSATTKFP N PPGFSQPLIQCDNSIQSP QKNSGRM AS VP TSLHL +SST VHK
Sbjct: 1141 LPSSATTKFPCNPPPGFSQPLIQCDNSIQSPVQKNSGRMTASVVPTTSLHLSSSSTPVHK 1200

Query: 1201 NSTQQKG-STPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVAP 1260
            NS QQKG STPQGQTQISF GGDFKPA+T MQHIPTS HSPSSSGKLRNT+SKT+PSV P
Sbjct: 1201 NSIQQKGSSTPQGQTQISFGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVTP 1260

Query: 1261 AQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            AQ KQDE SS+TGAGQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 AQQKQDE-SSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1277

BLAST of Lag0002401.1 vs. ExPASy Swiss-Prot
Match: Q94KE2 (Protein TIME FOR COFFEE OS=Arabidopsis thaliana OX=3702 GN=TIC PE=1 SV=2)

HSP 1 Score: 356.3 bits (913), Expect = 1.5e-96
Identity = 460/1455 (31.62%), Postives = 654/1455 (44.95%), Query Frame = 0

Query: 3    MEKSREARSSSSTVAANGSSRRRSRATAFRDLP-EEGQVELQETVRLRDRGG--KRDRDR 62
            M+++REAR      A NG SRRR RA +FRD P EEG VEL E  RLRDRGG  K+DRDR
Sbjct: 1    MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60

Query: 63   E-----------------FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVED 122
            E                   SRSKRRRG          D+GGD S+E+ V D   DE  D
Sbjct: 61   ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVND---DEEYD 120

Query: 123  VGGV------------SRILSSTTASSA-------------------SNQNQRRNSLPP- 182
             GGV            S  +S+ + SS+                   +N +QR+N+ PP 
Sbjct: 121  DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180

Query: 183  RVAKQQ------------WKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVG--GAG 242
            +V +              WKA D+ MIG  VPRKARSA  KR H+   S  +G G   +G
Sbjct: 181  KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240

Query: 243  EDIADNHSHRNLSD-SPAR--SSAEVV---------SPSTSIISAKKMKPTGPKTRSM-- 302
            E I     HR +S  SPA   S A ++         SPS+S IS +K  P+G K + +  
Sbjct: 241  EQI-----HRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPP 300

Query: 303  --KTSKSSSPSAKEGDIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVT 362
               +SK SSP A + +IEIEIAEVL+G+ + P  SK++      + N  TE +    +V 
Sbjct: 301  KSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVEV- 360

Query: 363  KSSVASATANSSQTAFQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKG 422
            KS V+S  +N  QT  Q S++L  N + S++   +A ++ K         N S     K 
Sbjct: 361  KSRVSSPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVKYEDDNSSRVTTIKS 420

Query: 423  ESEQPADMMEIYPQKLERASDLSMN---LFSHERKTEESKPTTVVYTGGDENKGSQKTVL 482
            E+E P+     +  +L+ +     N   L S    T ES   ++     + N    +T+L
Sbjct: 421  EAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESN-ASLDSEKKENNLSKDETIL 480

Query: 483  TQEDVASCAKGDVD-LEDSPPTKSIPVVETQKEEKFKFDLMAPP---TSPERDG--LADM 542
             + + +S  + D +  + S P K          EKF+ DLMAPP   +S ER G  +  +
Sbjct: 481  PKVESSSGFRSDGEGAKSSSPEK----------EKFEIDLMAPPPVRSSSERGGEMMECV 540

Query: 543  VLDAKPLSLGIEMGKETSNKVENEVVGF-----KEKEKVVDEDKMVTSGKNIEFFKLDLE 602
              +AKP    +E   +   K +           K++ ++V E +     +N E  KLDL+
Sbjct: 541  AAEAKPKVTEVETEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCE-LKLDLD 600

Query: 603  KPQLDGNCIIMQEQSQKQQLKGAAGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFR 662
            K    G  ++ +   QK   +      +K   ++ + L + + GWP   +P +GYM P +
Sbjct: 601  KSDHVG--LVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQ 660

Query: 663  TVLPVDSVDKPSTKLQ-HPNFILSHPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDAS 722
             V+P D+    +  +Q  P+ + + PRPKRC TH  +ARNI  HQQFTK +   P    S
Sbjct: 661  GVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGS 720

Query: 723  ASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANF 782
            A +   K  N+S    ++    L G+ L  S N VQ ++              S   +  
Sbjct: 721  APMYGTKACNLS----LMPPTELQGSVLGRSSNPVQDKN--------------SQSTSKS 780

Query: 783  ADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPIGQ--HQAPTAATGN----QSGSTKSSN 842
            ++ A+  QL+ QQ       NS++ G  FIFP+GQ  H A T A  +     SG T S  
Sbjct: 781  SETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGP 840

Query: 843  QSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSIEAPYLAKLQNKGYPFTFSTPAGT 902
             +TS   N  A       A PA +  MS+S+P +   E  YLA LQN GYPF      G 
Sbjct: 841  TATSTSMNGSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGA 900

Query: 903  SATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQSQHQPSGSHKQPQSQQQQWG---- 962
               YR    QP+P FNGSFY SQ+  P   QP Q Q Q Q +G   Q  +   Q G    
Sbjct: 901  QPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQP-QKQHQQQLTGQMLQSHAPNNQNGSAST 960

Query: 963  ------VHVPANNLVP------SSGMQVKQSTEQNLPMSNHQSRK----HESETSGEDAT 1022
                   H+    L P      S G    +   Q L     Q  +      SET GED+ 
Sbjct: 961  GSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSP 1020

Query: 1023 SLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGD--KKQSHTQNLKST 1082
            S  D R +     AYGQN+ + +Q     LM S     G  G++    +K+S  Q  K+ 
Sbjct: 1021 STADSRGSR-SNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAG 1080

Query: 1083 VALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQK 1142
            V    S G  M+FA++NG  T   LN + IAQN A+F ++P+  R G+Q+  A  A ++ 
Sbjct: 1081 VESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKM 1140

Query: 1143 NHQTS--EGRNGVVMSGCNDRNT--------GNSSAKPS-ATSGQTLVFGNSARTLNFMT 1202
            N+  S  +G++G +       NT        G +  K S    GQ++ F N     +   
Sbjct: 1141 NYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASV 1200

Query: 1203 SSV---PVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIM------------- 1262
            S+V    +   S    +  S   + +GS      QQ L Q  QQH+              
Sbjct: 1201 SAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYL 1260

Query: 1263 --QQQQATMAPRVAPRTKVPT----------NNTLPSSATTKFPYNAPPGFSQPLIQ-CD 1269
              QQ+ AT     A RTK P           N T   + TTKF  NA  GF Q L+Q   
Sbjct: 1261 QKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFA-NANSGFPQNLVQSSS 1320

BLAST of Lag0002401.1 vs. ExPASy TrEMBL
Match: A0A0A0K8W1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G388450 PE=4 SV=1)

HSP 1 Score: 1966.8 bits (5094), Expect = 0.0e+00
Identity = 1089/1303 (83.58%), Postives = 1153/1303 (88.49%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVED-VGGVSRILSS-TTASSA 120
            F SRSKRRR GGNREEE+EE+EGGDTSAEDIVADG  DEVED  GGVSRILSS TTASS 
Sbjct: 61   FVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADGDIDEVEDGGGGVSRILSSTTTASSV 120

Query: 121  SN--QNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAG 180
            SN  QNQ+R SLPPRV KQQWK  DDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAG
Sbjct: 121  SNQHQNQKRTSLPPRVVKQQWKVADDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAG 180

Query: 181  EDIADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEG 240
            EDIAD+HSHRN +DSPARSSAEVVSPSTSIISA KKMKPTGPKTRSMKTS  SS SAKEG
Sbjct: 181  EDIADDHSHRNQTDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEG 240

Query: 241  DIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTA 300
            DIEIEIAEVLFGLKKQP CSKKQEV  K SS QETENSSVLRD +KSSV S  ANS+QTA
Sbjct: 241  DIEIEIAEVLFGLKKQPHCSKKQEVITKQSSKQETENSSVLRDGSKSSVTSTMANSAQTA 300

Query: 301  FQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQK 360
            F KSVSLQKN  ISDL LNVA EK KV+SS       +LD A KGESE+PA+ + IYP K
Sbjct: 301  FNKSVSLQKNDVISDLSLNVAGEKQKVDSS-------TLDFAGKGESEKPAE-IAIYPSK 360

Query: 361  LERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDS 420
            LE AS             EESKP   ++TGGDENKGS+KT L QED+ SC KGDVD EDS
Sbjct: 361  LEGAS-------------EESKPAKEIFTGGDENKGSKKTGLAQEDITSCLKGDVDPEDS 420

Query: 421  PPTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENE 480
            PP  SIP   TQKEEKFKFDLMAPPTSPERDGLADMVLD KPLSLGIEMGKETSNKVENE
Sbjct: 421  PPNNSIPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSNKVENE 480

Query: 481  VVGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEK 540
            V GFKEKEKV++EDKMVTSG   EFFKLDLEKPQLD N I M+EQSQKQQ KGAA TVEK
Sbjct: 481  VEGFKEKEKVINEDKMVTSGTKFEFFKLDLEKPQLDSNNITMKEQSQKQQPKGAASTVEK 540

Query: 541  NESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKR 600
            NE STSVRLPIILGGWPTTEIP VGY+PPFRTVLPVDS+DK STKLQ+ NFILSHPRPKR
Sbjct: 541  NEPSTSVRLPIILGGWPTTEIPSVGYLPPFRTVLPVDSIDKSSTKLQNLNFILSHPRPKR 600

Query: 601  CLTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEV 660
            CLTHYD+ARNIYLHQQFTKT+Y HP GDASASL E KLKN+SSKEGM+LSN L GNHL++
Sbjct: 601  CLTHYDIARNIYLHQQFTKTNYFHPAGDASASLVEAKLKNISSKEGMLLSNQLSGNHLDM 660

Query: 661  SLNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFI 720
            +LNSVQQR+Q EGD  GNVVNDKS EAANFAD+AKSKQLVF Q+QG   GNSM P SGFI
Sbjct: 661  NLNSVQQREQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPLGNSM-PSSGFI 720

Query: 721  FPIGQHQAPTA-ATGNQSGSTKSS-NQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNV 780
            FPIGQHQAP A AT NQSGS KSS NQSTSLF+NPEAGTLVSFPA PAVS+NMSYSHPNV
Sbjct: 721  FPIGQHQAPIAQATANQSGSAKSSNNQSTSLFSNPEAGTLVSFPAFPAVSTNMSYSHPNV 780

Query: 781  VSIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQ 840
            VS+EAPYLAKLQN GYPFTFSTPAGTSATYR NNAQPLPLFNGSFYPSQLFHPSQIQPAQ
Sbjct: 781  VSVEAPYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYPSQLFHPSQIQPAQ 840

Query: 841  TQSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESET 900
            TQS HQPSGSHKQPQ+Q QQW VHVP NN++PS+GMQ+KQSTEQ+LP+SNH SRKHE+ET
Sbjct: 841  TQSHHQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQLKQSTEQHLPLSNH-SRKHENET 900

Query: 901  SGEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQN 960
            SGED TSL DKRAAPVQKNAYGQN+ILPVQALGFTLMPS T+NNGN+GN G+KKQSHTQN
Sbjct: 901  SGEDTTSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHTQN 960

Query: 961  LKSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQA 1020
            LK+TVALVPSQGLTMSFASYNGNGTPSNLNFTPI+QNT V+QNLPD+QR GFQVAP PQA
Sbjct: 961  LKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPISQNTTVYQNLPDIQRTGFQVAPVPQA 1020

Query: 1021 TKQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVN 1080
            TKQKNHQTSEGRNGVV+SG +DRNTGNSS KPS ATSGQTLVFGN +RTLNFMTSSVPVN
Sbjct: 1021 TKQKNHQTSEGRNGVVLSGASDRNTGNSSTKPSTATSGQTLVFGNPSRTLNFMTSSVPVN 1080

Query: 1081 WPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLP-QQHIMQQQQATMAPRVAPRTKVPTNN 1140
            WPSPS KSAA TTNRPAGSSSG+QQQQPLLQLP QQHI+QQQQA M PR+APRTKVPTNN
Sbjct: 1081 WPSPSNKSAA-TTNRPAGSSSGNQQQQPLLQLPQQQHILQQQQAAMGPRMAPRTKVPTNN 1140

Query: 1141 TLPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHL-ASSTAVH 1200
            TLPSSATTKFP N PPGFSQPLIQCDNSIQSPAQKNSGRM AS VPMTSLHL +SST VH
Sbjct: 1141 TLPSSATTKFPCNPPPGFSQPLIQCDNSIQSPAQKNSGRMTASVVPMTSLHLSSSSTPVH 1200

Query: 1201 KNSTQQKG-STPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVA 1260
            K+S QQKG STPQGQTQISF GGDFKPA+T MQHIPTS HSPSSSGKLRNT+SKT+PSV 
Sbjct: 1201 KSSIQQKGSSTPQGQTQISFGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVT 1260

Query: 1261 PAQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            P Q KQDE SS+TGAGQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 PTQQKQDE-SSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1278

BLAST of Lag0002401.1 vs. ExPASy TrEMBL
Match: A0A5A7V8Y6 (Protein TIME FOR COFFEE isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001730 PE=4 SV=1)

HSP 1 Score: 1958.7 bits (5073), Expect = 0.0e+00
Identity = 1087/1302 (83.49%), Postives = 1152/1302 (88.48%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKR+RDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRERDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVG-GVSRILSS-TTASSA 120
            F SRSKRRR GGNREEE+EE+EGGDTSAEDIVADG SD VED G GVSRILSS TTASS 
Sbjct: 61   FVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADGDSDGVEDGGVGVSRILSSTTTASSV 120

Query: 121  SNQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180
            SNQNQ+RNSLPPRV KQQWK  DDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGED
Sbjct: 121  SNQNQKRNSLPPRVVKQQWKVADDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180

Query: 181  IADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDI 240
            IAD+HSHRN +DSPARSSAEVVSPSTSIISA KKMKPTGPKTRSMKTS  SS SAKEGDI
Sbjct: 181  IADDHSHRNQTDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEGDI 240

Query: 241  EIEIAEVLFGLKKQPQ-CSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAF 300
            EIEIAEVLFGLKKQPQ CSKKQEVN+K SS QETENSSVLRD +KSS+ S  ANS+QTAF
Sbjct: 241  EIEIAEVLFGLKKQPQHCSKKQEVNMKQSSKQETENSSVLRDGSKSSITSTMANSAQTAF 300

Query: 301  QKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKL 360
             KSVSLQKN  ISDL LNVA EK KV+S         LD A KGESE+PA+ + IYP KL
Sbjct: 301  NKSVSLQKNDVISDLSLNVAGEKQKVDSL-------PLDFAVKGESEKPAE-IAIYPSKL 360

Query: 361  ERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSP 420
            E AS             EESKP   V+TGGDENKGS++T L QED+ SCAKGDVD EDSP
Sbjct: 361  EGAS-------------EESKPAKEVFTGGDENKGSKRTGLAQEDITSCAKGDVDPEDSP 420

Query: 421  PTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEV 480
            P KSIP   TQKEEKFKFDLMAPPTSPERDGLADMVLD KPLSLGIEMGKETS+KVENEV
Sbjct: 421  PNKSIPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSSKVENEV 480

Query: 481  VGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKN 540
             G KEKEKV++EDK+VTSG   EFFKLDLEKPQLD N I MQEQS KQQ KGAA TVEKN
Sbjct: 481  EGLKEKEKVINEDKIVTSGAKFEFFKLDLEKPQLDSNNITMQEQSPKQQPKGAASTVEKN 540

Query: 541  ESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRC 600
            E STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQH NFILSHPRPKRC
Sbjct: 541  EPSTSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHLNFILSHPRPKRC 600

Query: 601  LTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVS 660
            LTHYD+ARNIYLHQQFTKT+Y HP GDASASLAE KLKN+SSKEG +LSN L GNHL+V+
Sbjct: 601  LTHYDIARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNISSKEGTLLSNPLSGNHLDVN 660

Query: 661  LNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIF 720
            LNSV+Q++Q EGD  GNVVNDKS EAANFAD+AKSKQLVF Q+QG  FGNSM P SGFIF
Sbjct: 661  LNSVRQKEQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPFGNSM-PSSGFIF 720

Query: 721  PIGQHQAPTA-ATGNQSGSTKSS-NQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVV 780
            PIGQHQA  A AT NQSGS KSS NQSTSLF+NPE GTLVSFPA PAVS+NMSYSHPNVV
Sbjct: 721  PIGQHQASVAQATANQSGSAKSSNNQSTSLFSNPETGTLVSFPAFPAVSTNMSYSHPNVV 780

Query: 781  SIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQT 840
            S+EAPYLAKLQN GYPFTFSTPAGTSATYR NNAQPLPLFNGSFY SQ+FHPSQIQPAQT
Sbjct: 781  SVEAPYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYSSQMFHPSQIQPAQT 840

Query: 841  QSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETS 900
            QS HQPSGSHKQPQ+Q QQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETS
Sbjct: 841  QSHHQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETS 900

Query: 901  GEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNL 960
            GED TSL DKRAAPVQKNAYGQN+ILPVQALGFTLMPS T+NNGN+GN G+KKQSH QNL
Sbjct: 901  GEDTTSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHPQNL 960

Query: 961  KSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQAT 1020
            K+TVALVPSQGLTMSFASYNGNGTPSNLNF+PI+QNT V+QNLPD+QR GFQVAP PQAT
Sbjct: 961  KNTVALVPSQGLTMSFASYNGNGTPSNLNFSPISQNTTVYQNLPDIQRTGFQVAPVPQAT 1020

Query: 1021 KQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNW 1080
            KQKNHQTSEGRNGVV+SG +DRNTGNSS KPS ATSGQTLVFGN +RTLNFMTSSVPVNW
Sbjct: 1021 KQKNHQTSEGRNGVVLSGASDRNTGNSSTKPSAATSGQTLVFGNPSRTLNFMTSSVPVNW 1080

Query: 1081 PSPSTKSAASTTNRPAGSSSGHQQQQPLLQLP-QQHIMQQQQATMAPRVAPRTKVPTNNT 1140
            PSPSTKSAA TTNRPAGSSSG+QQQQPL QLP QQHI+QQQQA MAPR+APRTKVPTNNT
Sbjct: 1081 PSPSTKSAA-TTNRPAGSSSGNQQQQPLPQLPQQQHILQQQQAAMAPRMAPRTKVPTNNT 1140

Query: 1141 LPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHL-ASSTAVHK 1200
            LPSSATTKFP N PPGFSQPLIQCDNSIQSP QKNSGRM AS VP TSLHL +SST VHK
Sbjct: 1141 LPSSATTKFPCNPPPGFSQPLIQCDNSIQSPVQKNSGRMTASVVPTTSLHLSSSSTPVHK 1200

Query: 1201 NSTQQKG-STPQGQTQISF-GGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVAP 1260
            NS QQKG STPQGQTQISF GGDFKPA+T MQHIPTS HSPSSSGKLRNT+SKT+PSV P
Sbjct: 1201 NSIQQKGSSTPQGQTQISFGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVTP 1260

Query: 1261 AQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            AQ KQDE SS+TGAGQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 AQQKQDE-SSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1277

BLAST of Lag0002401.1 vs. ExPASy TrEMBL
Match: A0A1S3BSD8 (protein TIME FOR COFFEE isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492669 PE=4 SV=1)

HSP 1 Score: 1957.6 bits (5070), Expect = 0.0e+00
Identity = 1087/1303 (83.42%), Postives = 1152/1303 (88.41%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEK+REARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKR+RDRE
Sbjct: 1    MIMEKNREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRERDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVG-GVSRILSS-TTASSA 120
            F SRSKRRR GGNREEE+EE+EGGDTSAEDIVADG SD VED G GVSRILSS TTASS 
Sbjct: 61   FVSRSKRRREGGNREEEMEEEEGGDTSAEDIVADGDSDGVEDGGVGVSRILSSTTTASSV 120

Query: 121  SNQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180
            SNQNQ+RNSLPPRV KQQWK  DDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGED
Sbjct: 121  SNQNQKRNSLPPRVVKQQWKVADDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180

Query: 181  IADNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDI 240
            IAD+HSHRN +DSPARSSAEVVSPSTSIISA KKMKPTGPKTRSMKTS  SS SAKEGDI
Sbjct: 181  IADDHSHRNQTDSPARSSAEVVSPSTSIISAKKKMKPTGPKTRSMKTSNVSSASAKEGDI 240

Query: 241  EIEIAEVLFGLKKQPQ-CSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAF 300
            EIEIAEVLFGLKKQPQ CSKKQEVN+K SS QETENSSVLRD +KSS+ S  ANS+QTAF
Sbjct: 241  EIEIAEVLFGLKKQPQHCSKKQEVNMKQSSKQETENSSVLRDGSKSSITSTMANSAQTAF 300

Query: 301  QKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKL 360
             KSVSLQKN  ISDL LNVA EK KV+S         LD A KGESE+PA+ + IYP KL
Sbjct: 301  NKSVSLQKNDVISDLSLNVAGEKQKVDSL-------PLDFAVKGESEKPAE-IAIYPSKL 360

Query: 361  ERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDSP 420
            E AS             EESKP   V+TGGDENKGS++T L QED+ SCAKGDVD EDSP
Sbjct: 361  EGAS-------------EESKPAKEVFTGGDENKGSKRTGLAQEDITSCAKGDVDPEDSP 420

Query: 421  PTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEV 480
            P KSIP   TQKEEKFKFDLMAPPTSPERDGLADMVLD KPLSLGIEMGKETS+KVENEV
Sbjct: 421  PNKSIPEAVTQKEEKFKFDLMAPPTSPERDGLADMVLDTKPLSLGIEMGKETSSKVENEV 480

Query: 481  VGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKN 540
             G KEKEKV++EDK+VTSG   EFFKLDLEKPQLD N I MQEQS KQQ KGAA TVEKN
Sbjct: 481  EGLKEKEKVINEDKIVTSGTKFEFFKLDLEKPQLDSNNITMQEQSPKQQPKGAASTVEKN 540

Query: 541  ESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRC 600
            E STSVRLPIILGGWPTTEIP VGYMPPFRTVLPVDSVDK STKLQH NFILSHPRPKRC
Sbjct: 541  EPSTSVRLPIILGGWPTTEIPSVGYMPPFRTVLPVDSVDKSSTKLQHLNFILSHPRPKRC 600

Query: 601  LTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVS 660
            LTHYD+ARNIYLHQQFTKT+Y HP GDASASLAE KLKN+SSKEGM+LSN L GNHL+V+
Sbjct: 601  LTHYDIARNIYLHQQFTKTNYFHPAGDASASLAEAKLKNISSKEGMLLSNPLSGNHLDVN 660

Query: 661  LNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIF 720
            LNSV+Q++Q EGD  GNVVNDKS EAANFAD+AKSKQLVF Q+QG  FGNSM P SGFIF
Sbjct: 661  LNSVRQKEQGEGDLPGNVVNDKSSEAANFADIAKSKQLVFHQKQGVPFGNSM-PSSGFIF 720

Query: 721  PIGQHQAPTA-ATGNQSGSTKSS-NQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVV 780
            PIGQHQA  A AT NQSGS KSS NQSTSLF+NPE GTLVSFPA PAVS+NMSYSHPNVV
Sbjct: 721  PIGQHQASVAQATANQSGSAKSSNNQSTSLFSNPETGTLVSFPAFPAVSTNMSYSHPNVV 780

Query: 781  SIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQT 840
            S+EAPYLAKLQN GYPFTFSTPAGTSATYR NNAQPLPLFNGSFY SQ+FHPSQIQPAQT
Sbjct: 781  SVEAPYLAKLQNNGYPFTFSTPAGTSATYRTNNAQPLPLFNGSFYSSQMFHPSQIQPAQT 840

Query: 841  QSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKHESETS 900
            QS HQPSGSHKQPQ+Q QQW VHVP NN++PS+GMQVKQSTEQ+LP+SN QSRKHE+ETS
Sbjct: 841  QSHHQPSGSHKQPQTQPQQWSVHVPGNNVLPSNGMQVKQSTEQHLPLSN-QSRKHENETS 900

Query: 901  GEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNL 960
            GED TSL DKRAAPVQKNAYGQN+ILPVQALGFTLMPS T+NNGN+GN G+KKQSH QNL
Sbjct: 901  GEDTTSLSDKRAAPVQKNAYGQNYILPVQALGFTLMPSTTLNNGNSGNYGEKKQSHPQNL 960

Query: 961  KSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQAT 1020
            K+TVALVPSQGLTMSFASYNGNGTPSNLNF+PI+QNT V+QNLPD+QR GFQVA  PQAT
Sbjct: 961  KNTVALVPSQGLTMSFASYNGNGTPSNLNFSPISQNTTVYQNLPDIQRTGFQVATVPQAT 1020

Query: 1021 KQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPS-ATSGQTLVFGNSARTLNFMTSSVPVNW 1080
            KQKNHQTSEGRNGVV+SG +DRNTGNSS KPS ATSGQTLVFGN +RTLNFMTSSVPVNW
Sbjct: 1021 KQKNHQTSEGRNGVVLSGASDRNTGNSSTKPSAATSGQTLVFGNPSRTLNFMTSSVPVNW 1080

Query: 1081 PSPSTKSAASTTNRPAGSSSGHQQQQPLLQLP-QQHIMQQQQATMAPRVAPRTKVPTNNT 1140
            PSPSTKSAA TTNRPAGSSSG+QQQQPL QLP QQHI+QQQQA MAPR+APRTKVPTNNT
Sbjct: 1081 PSPSTKSAA-TTNRPAGSSSGNQQQQPLPQLPQQQHILQQQQAAMAPRMAPRTKVPTNNT 1140

Query: 1141 LPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHL-ASSTAVHK 1200
            LPSSATTKFP N PPGFSQPLIQCDNSIQSP QKNSGRM AS VP TSLHL +SST VHK
Sbjct: 1141 LPSSATTKFPCNPPPGFSQPLIQCDNSIQSPVQKNSGRMTASVVPTTSLHLSSSSTPVHK 1200

Query: 1201 NSTQQKG-STPQGQTQISF--GGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVA 1260
            NS QQKG STPQGQTQISF  GGDFKPA+T MQHIPTS HSPSSSGKLRNT+SKT+PSV 
Sbjct: 1201 NSIQQKGSSTPQGQTQISFGGGGDFKPAYTPMQHIPTSGHSPSSSGKLRNTTSKTNPSVT 1260

Query: 1261 PAQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
            PAQ KQDE SS+TGAGQKSSPVCGRNVPSILNTCPS LSELKY
Sbjct: 1261 PAQQKQDE-SSSTGAGQKSSPVCGRNVPSILNTCPSQLSELKY 1278

BLAST of Lag0002401.1 vs. ExPASy TrEMBL
Match: A0A6J1GN28 (protein TIME FOR COFFEE-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456008 PE=4 SV=1)

HSP 1 Score: 1945.2 bits (5038), Expect = 0.0e+00
Identity = 1076/1295 (83.09%), Postives = 1134/1295 (87.57%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRGGKRDRDRE 60
            MIMEKSREARSSSS VAANGSSRRRSRATAFRDLPEEGQVELQETVRLR+RGGKR RDRE
Sbjct: 1    MIMEKSREARSSSSIVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRERGGKRGRDRE 60

Query: 61   FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRILSSTTASSASN 120
            FASRSKR RGGGNREEEVEE EGGDTSAEDIVADG SDEVED GGVS ILSSTTA+SASN
Sbjct: 61   FASRSKRSRGGGNREEEVEE-EGGDTSAEDIVADGDSDEVEDGGGVSLILSSTTAASASN 120

Query: 121  QNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGEDIA 180
            QNQ+RNSLPPRVAKQQWK VDDAMIG PVPRKARSASVKRSHDCTVSGNSGVGGAGEDIA
Sbjct: 121  QNQKRNSLPPRVAKQQWKVVDDAMIGVPVPRKARSASVKRSHDCTVSGNSGVGGAGEDIA 180

Query: 181  DNHSHRNLSDSPARSSAEVVSPSTSIISA-KKMKPTGPKTRSMKTSKSSSPSAKEGDIEI 240
            D+HSHRN SDSPARSSAEVVSPSTS++S+ KKMK +GPKTRSMKTSK SSPSA EGDIEI
Sbjct: 181  DDHSHRNQSDSPARSSAEVVSPSTSVVSSKKKMKSSGPKTRSMKTSKGSSPSAMEGDIEI 240

Query: 241  EIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAFQKS 300
            EIAEVLFGLKKQPQCSKKQEVN+ PSSNQETE+SSVLRD TK  V S  ANS+QTAFQKS
Sbjct: 241  EIAEVLFGLKKQPQCSKKQEVNMNPSSNQETEDSSVLRDGTKPPVTSTAANSAQTAFQKS 300

Query: 301  VSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQKLERA 360
            VSLQKN AISDLLLNVAVEK            +SLDGAAK ESE+PA+ +EIYP KL  A
Sbjct: 301  VSLQKNDAISDLLLNVAVEK-----------TESLDGAAKAESEKPAE-IEIYPSKLGGA 360

Query: 361  SDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLED-SPPT 420
            SD S+NL SHERKTEE KP   V T GDENK S+KTVL QEDV SC KGDVD ED SPPT
Sbjct: 361  SDFSLNLGSHERKTEEMKPEKEVCTSGDENKESKKTVLVQEDVTSCVKGDVDPEDLSPPT 420

Query: 421  KSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNKVENEVVG 480
            KSIP  ETQKEEKFKFDLMAPP SPERDGLADMVLDAKPLSLGI+MGKETSNKVEN    
Sbjct: 421  KSIPEAETQKEEKFKFDLMAPPASPERDGLADMVLDAKPLSLGIQMGKETSNKVEN---- 480

Query: 481  FKEKEKVVDEDKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGAAGTVEKNES 540
                     ED +VTSG   EFFKLDLEKPQLD N I MQEQSQKQQ KGAAGTVE N+ 
Sbjct: 481  ---------EDSIVTSGTKFEFFKLDLEKPQLDRNSIAMQEQSQKQQPKGAAGTVENNDP 540

Query: 541  STSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILSHPRPKRCLT 600
            STSVRLP+ILGGWPTTEIPPVGYMPPFRTVLPVD VDK STKLQHP FILSHPRPKRCLT
Sbjct: 541  STSVRLPVILGGWPTTEIPPVGYMPPFRTVLPVDIVDKSSTKLQHPKFILSHPRPKRCLT 600

Query: 601  HYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLN 660
            HYD+ARNIYLHQQFTKT+Y HP+GDASASLAE KLKNMSSKEGMILSN LGGNHL+++LN
Sbjct: 601  HYDIARNIYLHQQFTKTNYFHPSGDASASLAEAKLKNMSSKEGMILSNPLGGNHLDMNLN 660

Query: 661  SVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPI 720
            S +Q++Q E DF GNVVNDK PEAA  ADMAKSKQLVFQQ QGASFGNSM PGSGFIFPI
Sbjct: 661  SARQKEQAEVDFPGNVVNDKVPEAAYLADMAKSKQLVFQQGQGASFGNSM-PGSGFIFPI 720

Query: 721  GQHQAPTAATGNQSGSTKSSNQSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSIEA 780
             QHQAPTAAT NQSGSTKSSNQST LF NPEAGTLVSFPA PAVSSNMSY+HPNVVSIEA
Sbjct: 721  SQHQAPTAATANQSGSTKSSNQSTPLFTNPEAGTLVSFPAFPAVSSNMSYTHPNVVSIEA 780

Query: 781  PYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQSQH 840
            PYL K QN  YPFTFSTPAGTSATYRA++AQPLPLFNGSFYPSQLFHPSQIQPAQTQS H
Sbjct: 781  PYLGKFQNNSYPFTFSTPAGTSATYRASSAQPLPLFNGSFYPSQLFHPSQIQPAQTQSHH 840

Query: 841  QPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVK-QSTEQNLPMSNHQSRKHESETSGED 900
            QPSGSHKQPQ+QQQQW VHVP NN+ PS+GMQVK QSTEQ+LP+   QSRKHE+ETSGED
Sbjct: 841  QPSGSHKQPQTQQQQWSVHVPGNNVPPSNGMQVKQQSTEQHLPLPK-QSRKHENETSGED 900

Query: 901  ATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQSHTQNLKST 960
            +TSL D R A VQK+AYGQNFILPV ALGFTLMPS T NNGN GN+G+KKQSH QNLKST
Sbjct: 901  STSLSDMRPASVQKSAYGQNFILPVPALGFTLMPSGTFNNGNGGNSGEKKQSHPQNLKST 960

Query: 961  VALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQK 1020
            VALVPSQGLTMSFASYNGNGTPSNLNFTP      VFQNLPDMQR GFQ APAPQATKQK
Sbjct: 961  VALVPSQGLTMSFASYNGNGTPSNLNFTP-----TVFQNLPDMQRTGFQGAPAPQATKQK 1020

Query: 1021 NHQTSEGRNGVVMSGCNDRNTGNSSAKPSATSGQTLVFGNSARTLNFMTSSVPVNWPSPS 1080
            NHQ SEGRNGVV+SG +DRNTGNSSAKPSATSGQTLVFGNS+RTLNFMTSSVPVNWPSP 
Sbjct: 1021 NHQISEGRNGVVVSGTSDRNTGNSSAKPSATSGQTLVFGNSSRTLNFMTSSVPVNWPSP- 1080

Query: 1081 TKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQQQQATMAPRVAPRTKVPTNNTLPSSA 1140
                 STTNRPAGSSSG+Q         QQHI+QQQQATM     PR+K P+NN+LPSSA
Sbjct: 1081 -----STTNRPAGSSSGNQ---------QQHILQQQQATM----VPRSKGPSNNSLPSSA 1140

Query: 1141 TTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHLASSTAVHKNSTQQK 1200
            TTKFP +APPGFSQPLIQCDNSIQSPAQKNSGRMV + VPMTSLHLASSTAVHK+STQQK
Sbjct: 1141 TTKFPCSAPPGFSQPLIQCDNSIQSPAQKNSGRMVGTVVPMTSLHLASSTAVHKSSTQQK 1200

Query: 1201 G-STPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVAPAQPKQDE 1260
            G +TP GQTQISFGGDFKP FTQMQH+PT  HSPSSSGKLRNTSSKTSPSVAP QPKQDE
Sbjct: 1201 GNNTPHGQTQISFGGDFKPGFTQMQHLPTGGHSPSSSGKLRNTSSKTSPSVAPVQPKQDE 1242

Query: 1261 GSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
             SS+TGAG+KSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 -SSSTGAGKKSSPVCGRNVPSILNTCPSHLSELKY 1242

BLAST of Lag0002401.1 vs. ExPASy TrEMBL
Match: A0A6J1DB26 (protein TIME FOR COFFEE isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018485 PE=4 SV=1)

HSP 1 Score: 1934.5 bits (5010), Expect = 0.0e+00
Identity = 1072/1302 (82.33%), Postives = 1135/1302 (87.17%), Query Frame = 0

Query: 1    MIMEKSREARSSSSTVAANGSSRRRSRATAFRDLPEEGQVELQETVRLRDRG-GKRDRDR 60
            MIMEK+REARSSSSTVAANGSSRRRSRAT FRDLPEEGQVELQETVRLRD+G GKRDRDR
Sbjct: 1    MIMEKTREARSSSSTVAANGSSRRRSRATVFRDLPEEGQVELQETVRLRDKGAGKRDRDR 60

Query: 61   EFASRSKRRRGGGNREEEVEEDEGGDTSAEDIVAD-GYSDEVEDVGGVSRILSSTTASSA 120
            EFASRSKRRRGG NREEEVEEDEGGDTSAED+V D G SDEVED GGVSRILSS TASSA
Sbjct: 61   EFASRSKRRRGGANREEEVEEDEGGDTSAEDVVVDGGDSDEVEDGGGVSRILSSATASSA 120

Query: 121  SNQNQRRNSLPPRVAKQQWKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAGED 180
            SNQN R NSLPPRVAKQQWK VDDAMIG PVPRKAR+ASVKRSHDCTVSGNSGVGGAGED
Sbjct: 121  SNQNLRMNSLPPRVAKQQWKVVDDAMIGVPVPRKARTASVKRSHDCTVSGNSGVGGAGED 180

Query: 181  IADNHSHRNLSDSPARSSAEVVSPSTSIISAKKMKPTGPKTRSMKTSKSSSPSAKEGDIE 240
            IAD+ SHRN SDSPARSSAEVVSP  SI + KKMKPTGPKTR+MK SK SSPS  EGDIE
Sbjct: 181  IADDPSHRNPSDSPARSSAEVVSP--SIFAKKKMKPTGPKTRTMKASKDSSPST-EGDIE 240

Query: 241  IEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTAFQK 300
            IEIAEVLFGLKKQPQCS+KQE+  KPSSNQETENSS LR+ TKS V S TANS+QT+FQK
Sbjct: 241  IEIAEVLFGLKKQPQCSRKQEITAKPSSNQETENSSALREGTKSPVTSTTANSAQTSFQK 300

Query: 301  SVSLQKNGAISDLLLNVAVEKNKVE--SSSSLVQNDSLDGAAKGESEQPADMMEIYPQKL 360
            SVSLQKNGAISDLLLNVAVEKNK E  SSS L ++DS+ GAAKGESEQ A         +
Sbjct: 301  SVSLQKNGAISDLLLNVAVEKNKSEHDSSSPLARSDSMAGAAKGESEQHAG--------I 360

Query: 361  ERASDLSMNLFSH-----ERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVD 420
            E ASD  MNL SH       K EESK T   +TGGDEN GS+KTV TQ+D+ SC KGDV 
Sbjct: 361  EGASDFGMNLGSHGCQTVVTKPEESKLTKEFFTGGDENPGSKKTVSTQDDLPSCIKGDVG 420

Query: 421  LEDSPPTKSIPVVETQKEEKFKFDLMAPPTSPERDGLADMVLDAKPLSLGIEMGKETSNK 480
             EDSPP+KSIP  ETQKEEKFKFDLMAPPTSPERDGL DMVLD KPLSLG+EMGK TS+K
Sbjct: 421  PEDSPPSKSIPEAETQKEEKFKFDLMAPPTSPERDGLTDMVLDIKPLSLGLEMGKGTSHK 480

Query: 481  VENEVVGFKEKEKVVDE--DKMVTSGKNIEFFKLDLEKPQLDGNCIIMQEQSQKQQLKGA 540
            VENEV GFKEKEKV+DE  DK+V+SGK  +FFKLDLE+PQLD N + MQEQSQKQQ KGA
Sbjct: 481  VENEVEGFKEKEKVIDEVRDKVVSSGKKFDFFKLDLERPQLDSNSLTMQEQSQKQQHKGA 540

Query: 541  AGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQHPNFILS 600
            AGTVEKNE STSVRLPI LGGWPT EIPPVGYMPPFRT+L VDS DK S+KLQHPNFILS
Sbjct: 541  AGTVEKNEPSTSVRLPITLGGWPTAEIPPVGYMPPFRTMLSVDSGDKSSSKLQHPNFILS 600

Query: 601  HPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGMILSNSLG 660
            HPR KRCLTHYD+ARNIYLHQQFTKT+Y HP  DAS SLAE KLKN+SSKEG+ L N L 
Sbjct: 601  HPRSKRCLTHYDIARNIYLHQQFTKTNYFHPAADASTSLAEAKLKNLSSKEGISLGNPLH 660

Query: 661  GNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGASFGNSMV 720
             + L+++LN+     Q EGDF GNVVNDKSPEAANFADMAKSKQLVFQQR G SFGNSM 
Sbjct: 661  RHLLDMNLNTA----QAEGDFPGNVVNDKSPEAANFADMAKSKQLVFQQRPGPSFGNSM- 720

Query: 721  PGSGFIFPIGQHQAPTAATGNQSGSTKSSNQSTSLFNNPEAGTLVSFPALPAVSSNMSYS 780
            PGSGFIFPI QHQA  AAT NQSG+TKSSNQSTSLF+NPEAGTLVSFPALPAVSSNMSYS
Sbjct: 721  PGSGFIFPISQHQASIAATANQSGTTKSSNQSTSLFSNPEAGTLVSFPALPAVSSNMSYS 780

Query: 781  HPNVVSIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQLFHPSQI 840
            HPN+VSIEAPYLAKLQN GYPFTFSTPAGTS TYRAN+AQPLPLFNGSFYPSQLFHPSQI
Sbjct: 781  HPNMVSIEAPYLAKLQNNGYPFTFSTPAGTSTTYRANSAQPLPLFNGSFYPSQLFHPSQI 840

Query: 841  QPAQTQSQHQPSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQSTEQNLPMSNHQSRKH 900
            QPAQTQS HQ S SHKQPQ+QQQQW VHVP NNLVPS+ MQVKQSTEQ+LP+SN QSRKH
Sbjct: 841  QPAQTQSHHQSSSSHKQPQAQQQQWSVHVPGNNLVPSNAMQVKQSTEQHLPLSN-QSRKH 900

Query: 901  ESETSGEDATSLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGDKKQS 960
            + ET GED  +L DKR APVQKN YGQNFILPVQALGFTLMPSATMNN N GNNG+KKQS
Sbjct: 901  DGETGGEDTAALSDKRVAPVQKNTYGQNFILPVQALGFTLMPSATMNNANGGNNGEKKQS 960

Query: 961  HTQNLKSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAP 1020
            H QNLK+TVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQN  V+QNLPDMQRAGFQVAP
Sbjct: 961  HPQNLKNTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNATVYQNLPDMQRAGFQVAP 1020

Query: 1021 APQATKQKNHQTSEGRNGVVMSGCNDRNTGNSSAKPSATSGQTLVFGNSARTLNFMTSSV 1080
            APQATKQK+HQ+SEGRNGVV+SG NDRN GN SAKPS+TSGQTLVF NS RTLNFMTSS 
Sbjct: 1021 APQATKQKSHQSSEGRNGVVLSGSNDRNNGN-SAKPSSTSGQTLVFSNSGRTLNFMTSSA 1080

Query: 1081 PVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQQQQATMAPRVAPRTKVPT 1140
            PVNWPSPSTKSAA TTNR A SSSG+QQQQPLLQLPQQHI+QQQQATM      RTKVP 
Sbjct: 1081 PVNWPSPSTKSAALTTNRSAASSSGNQQQQPLLQLPQQHILQQQQATM----MSRTKVPA 1140

Query: 1141 NNTLPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRMVASGVPMTSLHLASSTAV 1200
            NN+LPSS   KFPYNAPPGFSQPL+ CD+S    AQKNSGRMVAS VPMTSLHLASS AV
Sbjct: 1141 NNSLPSSVGPKFPYNAPPGFSQPLMHCDDS----AQKNSGRMVASVVPMTSLHLASSPAV 1200

Query: 1201 HKNSTQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNTSSKTSPSVAP 1260
             KNS QQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNT SKTS SVAP
Sbjct: 1201 SKNSPQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSPSSSGKLRNTGSKTSSSVAP 1260

Query: 1261 AQPKQDEGSSTTGAGQKSSPVCGRNVPSILNTCPSHLSELKY 1292
             QPKQDE SS+TG GQKSSPVCGRNVPSILNTCPSHLSELKY
Sbjct: 1261 PQPKQDE-SSSTGGGQKSSPVCGRNVPSILNTCPSHLSELKY 1275

BLAST of Lag0002401.1 vs. TAIR 10
Match: AT3G22380.1 (time for coffee )

HSP 1 Score: 356.3 bits (913), Expect = 1.0e-97
Identity = 460/1455 (31.62%), Postives = 654/1455 (44.95%), Query Frame = 0

Query: 3    MEKSREARSSSSTVAANGSSRRRSRATAFRDLP-EEGQVELQETVRLRDRGG--KRDRDR 62
            M+++REAR      A NG SRRR RA +FRD P EEG VEL E  RLRDRGG  K+DRDR
Sbjct: 1    MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60

Query: 63   E-----------------FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVED 122
            E                   SRSKRRRG          D+GGD S+E+ V D   DE  D
Sbjct: 61   ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVND---DEEYD 120

Query: 123  VGGV------------SRILSSTTASSA-------------------SNQNQRRNSLPP- 182
             GGV            S  +S+ + SS+                   +N +QR+N+ PP 
Sbjct: 121  DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180

Query: 183  RVAKQQ------------WKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVG--GAG 242
            +V +              WKA D+ MIG  VPRKARSA  KR H+   S  +G G   +G
Sbjct: 181  KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240

Query: 243  EDIADNHSHRNLSD-SPAR--SSAEVV---------SPSTSIISAKKMKPTGPKTRSM-- 302
            E I     HR +S  SPA   S A ++         SPS+S IS +K  P+G K + +  
Sbjct: 241  EQI-----HRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPP 300

Query: 303  --KTSKSSSPSAKEGDIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVT 362
               +SK SSP A + +IEIEIAEVL+G+ + P  SK++      + N  TE +    +V 
Sbjct: 301  KSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVEV- 360

Query: 363  KSSVASATANSSQTAFQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKG 422
            KS V+S  +N  QT  Q S++L  N + S++   +A ++ K         N S     K 
Sbjct: 361  KSRVSSPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVKYEDDNSSRVTTIKS 420

Query: 423  ESEQPADMMEIYPQKLERASDLSMN---LFSHERKTEESKPTTVVYTGGDENKGSQKTVL 482
            E+E P+     +  +L+ +     N   L S    T ES   ++     + N    +T+L
Sbjct: 421  EAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESN-ASLDSEKKENNLSKDETIL 480

Query: 483  TQEDVASCAKGDVD-LEDSPPTKSIPVVETQKEEKFKFDLMAPP---TSPERDG--LADM 542
             + + +S  + D +  + S P K          EKF+ DLMAPP   +S ER G  +  +
Sbjct: 481  PKVESSSGFRSDGEGAKSSSPEK----------EKFEIDLMAPPPVRSSSERGGEMMECV 540

Query: 543  VLDAKPLSLGIEMGKETSNKVENEVVGF-----KEKEKVVDEDKMVTSGKNIEFFKLDLE 602
              +AKP    +E   +   K +           K++ ++V E +     +N E  KLDL+
Sbjct: 541  AAEAKPKVTEVETEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCE-LKLDLD 600

Query: 603  KPQLDGNCIIMQEQSQKQQLKGAAGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFR 662
            K    G  ++ +   QK   +      +K   ++ + L + + GWP   +P +GYM P +
Sbjct: 601  KSDHVG--LVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQ 660

Query: 663  TVLPVDSVDKPSTKLQ-HPNFILSHPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDAS 722
             V+P D+    +  +Q  P+ + + PRPKRC TH  +ARNI  HQQFTK +   P    S
Sbjct: 661  GVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGS 720

Query: 723  ASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANF 782
            A +   K  N+S    ++    L G+ L  S N VQ ++              S   +  
Sbjct: 721  APMYGTKACNLS----LMPPTELQGSVLGRSSNPVQDKN--------------SQSTSKS 780

Query: 783  ADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPIGQ--HQAPTAATGN----QSGSTKSSN 842
            ++ A+  QL+ QQ       NS++ G  FIFP+GQ  H A T A  +     SG T S  
Sbjct: 781  SETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGP 840

Query: 843  QSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSIEAPYLAKLQNKGYPFTFSTPAGT 902
             +TS   N  A       A PA +  MS+S+P +   E  YLA LQN GYPF      G 
Sbjct: 841  TATSTSMNGSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGA 900

Query: 903  SATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQSQHQPSGSHKQPQSQQQQWG---- 962
               YR    QP+P FNGSFY SQ+  P   QP Q Q Q Q +G   Q  +   Q G    
Sbjct: 901  QPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQP-QKQHQQQLTGQMLQSHAPNNQNGSAST 960

Query: 963  ------VHVPANNLVP------SSGMQVKQSTEQNLPMSNHQSRK----HESETSGEDAT 1022
                   H+    L P      S G    +   Q L     Q  +      SET GED+ 
Sbjct: 961  GSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSP 1020

Query: 1023 SLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGD--KKQSHTQNLKST 1082
            S  D R +     AYGQN+ + +Q     LM S     G  G++    +K+S  Q  K+ 
Sbjct: 1021 STADSRGSR-SNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAG 1080

Query: 1083 VALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQK 1142
            V    S G  M+FA++NG  T   LN + IAQN A+F ++P+  R G+Q+  A  A ++ 
Sbjct: 1081 VESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKM 1140

Query: 1143 NHQTS--EGRNGVVMSGCNDRNT--------GNSSAKPS-ATSGQTLVFGNSARTLNFMT 1202
            N+  S  +G++G +       NT        G +  K S    GQ++ F N     +   
Sbjct: 1141 NYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASV 1200

Query: 1203 SSV---PVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIM------------- 1262
            S+V    +   S    +  S   + +GS      QQ L Q  QQH+              
Sbjct: 1201 SAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYL 1260

Query: 1263 --QQQQATMAPRVAPRTKVPT----------NNTLPSSATTKFPYNAPPGFSQPLIQ-CD 1269
              QQ+ AT     A RTK P           N T   + TTKF  NA  GF Q L+Q   
Sbjct: 1261 QKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFA-NANSGFPQNLVQSSS 1320

BLAST of Lag0002401.1 vs. TAIR 10
Match: AT3G22380.2 (time for coffee )

HSP 1 Score: 356.3 bits (913), Expect = 1.0e-97
Identity = 460/1455 (31.62%), Postives = 654/1455 (44.95%), Query Frame = 0

Query: 3    MEKSREARSSSSTVAANGSSRRRSRATAFRDLP-EEGQVELQETVRLRDRGG--KRDRDR 62
            M+++REAR      A NG SRRR RA +FRD P EEG VEL E  RLRDRGG  K+DRDR
Sbjct: 1    MDRNREARRVPMAAAGNGLSRRRHRAGSFRDSPEEEGPVELPEAARLRDRGGSNKKDRDR 60

Query: 63   E-----------------FASRSKRRRGGGNREEEVEEDEGGDTSAEDIVADGYSDEVED 122
            E                   SRSKRRRG          D+GGD S+E+ V D   DE  D
Sbjct: 61   ERDRDRERERERDRERDRLNSRSKRRRGERLMMVHGNLDDGGDDSSEESVND---DEEYD 120

Query: 123  VGGV------------SRILSSTTASSA-------------------SNQNQRRNSLPP- 182
             GGV            S  +S+ + SS+                   +N +QR+N+ PP 
Sbjct: 121  DGGVGPPSSLKMLPPTSNNISAASFSSSLSNHHNGGSGNLHHHHHSHNNNHQRKNNFPPT 180

Query: 183  RVAKQQ------------WKAVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVG--GAG 242
            +V +              WKA D+ MIG  VPRKARSA  KR H+   S  +G G   +G
Sbjct: 181  KVFRSSPSPAPVSPLVSTWKAADE-MIGVSVPRKARSACTKRPHESWASSTTGGGVFASG 240

Query: 243  EDIADNHSHRNLSD-SPAR--SSAEVV---------SPSTSIISAKKMKPTGPKTRSM-- 302
            E I     HR +S  SPA   S A ++         SPS+S IS +K  P+G K + +  
Sbjct: 241  EQI-----HRQISSTSPANRVSPASILASPSPPAPTSPSSSSISVRKKLPSGTKQKPLPP 300

Query: 303  --KTSKSSSPSAKEGDIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVT 362
               +SK SSP A + +IEIEIAEVL+G+ + P  SK++      + N  TE +    +V 
Sbjct: 301  KSSSSKLSSPVAVQDEIEIEIAEVLYGMMRMPSTSKQE-----AAGNDLTEAAKSTVEV- 360

Query: 363  KSSVASATANSSQTAFQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKG 422
            KS V+S  +N  QT  Q S++L  N + S++   +A ++ K         N S     K 
Sbjct: 361  KSRVSSPISN-PQTLPQSSITLAANSSSSNVSA-IAPKRKKPRHVKYEDDNSSRVTTIKS 420

Query: 423  ESEQPADMMEIYPQKLERASDLSMN---LFSHERKTEESKPTTVVYTGGDENKGSQKTVL 482
            E+E P+     +  +L+ +     N   L S    T ES   ++     + N    +T+L
Sbjct: 421  EAEAPSKSQVPFSNQLKSSGSGEGNSSVLDSIIPLTRESN-ASLDSEKKENNLSKDETIL 480

Query: 483  TQEDVASCAKGDVD-LEDSPPTKSIPVVETQKEEKFKFDLMAPP---TSPERDG--LADM 542
             + + +S  + D +  + S P K          EKF+ DLMAPP   +S ER G  +  +
Sbjct: 481  PKVESSSGFRSDGEGAKSSSPEK----------EKFEIDLMAPPPVRSSSERGGEMMECV 540

Query: 543  VLDAKPLSLGIEMGKETSNKVENEVVGF-----KEKEKVVDEDKMVTSGKNIEFFKLDLE 602
              +AKP    +E   +   K +           K++ ++V E +     +N E  KLDL+
Sbjct: 541  AAEAKPKVTEVETEAKPLLKEDRSDPAIHDSQEKKRPRMVAEAEHHKFERNCE-LKLDLD 600

Query: 603  KPQLDGNCIIMQEQSQKQQLKGAAGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFR 662
            K    G  ++ +   QK   +      +K   ++ + L + + GWP   +P +GYM P +
Sbjct: 601  KSDHVG--LVNKHHVQKPPPQQQLSVPDKTAQASHLPLHMSMPGWP-GGLPTMGYMAPTQ 660

Query: 663  TVLPVDSVDKPSTKLQ-HPNFILSHPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDAS 722
             V+P D+    +  +Q  P+ + + PRPKRC TH  +ARNI  HQQFTK +   P    S
Sbjct: 661  GVVPTDTSSLSAAAMQPPPHLLFNQPRPKRCATHCYIARNIQSHQQFTKMNPFWPAAAGS 720

Query: 723  ASLAEVKLKNMSSKEGMILSNSLGGNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANF 782
            A +   K  N+S    ++    L G+ L  S N VQ ++              S   +  
Sbjct: 721  APMYGTKACNLS----LMPPTELQGSVLGRSSNPVQDKN--------------SQSTSKS 780

Query: 783  ADMAKSKQLVFQQRQGASFGNSMVPGSGFIFPIGQ--HQAPTAATGN----QSGSTKSSN 842
            ++ A+  QL+ QQ       NS++ G  FIFP+GQ  H A T A  +     SG T S  
Sbjct: 781  SETAQRNQLMLQQALPPGAANSILHGPTFIFPLGQQPHAAATIAAASVRPPNSGITSSGP 840

Query: 843  QSTSLFNNPEAGTLVSFPALPAVSSNMSYSHPNVVSIEAPYLAKLQNKGYPFTFSTPAGT 902
             +TS   N  A       A PA +  MS+S+P +   E  YLA LQN GYPF      G 
Sbjct: 841  TATSTSMNGSAS------ATPAGAPTMSFSYPAMPGNETQYLAILQNNGYPFPVPAHVGA 900

Query: 903  SATYRANNAQPLPLFNGSFYPSQLFHPSQIQPAQTQSQHQPSGSHKQPQSQQQQWG---- 962
               YR    QP+P FNGSFY SQ+  P   QP Q Q Q Q +G   Q  +   Q G    
Sbjct: 901  QPAYRGAPGQPMPFFNGSFYSSQMIQPPHHQP-QKQHQQQLTGQMLQSHAPNNQNGSAST 960

Query: 963  ------VHVPANNLVP------SSGMQVKQSTEQNLPMSNHQSRK----HESETSGEDAT 1022
                   H+    L P      S G    +   Q L     Q  +      SET GED+ 
Sbjct: 961  GSSAAQKHLQNQQLRPPINHGNSQGFPTHKVQSQPLNFQQRQQPRENATQHSETVGEDSP 1020

Query: 1023 SLPDKRAAPVQKNAYGQNFILPVQALGFTLMPSATMNNGNAGNNGD--KKQSHTQNLKST 1082
            S  D R +     AYGQN+ + +Q     LM S     G  G++    +K+S  Q  K+ 
Sbjct: 1021 STADSRGSR-SNVAYGQNYGMQMQPTNLGLMSSPAPGGGVVGSSSSHGEKKSQQQVSKAG 1080

Query: 1083 VALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAVFQNLPDMQRAGFQVAPAPQATKQK 1142
            V    S G  M+FA++NG  T   LN + IAQN A+F ++P+  R G+Q+  A  A ++ 
Sbjct: 1081 VESFQSPGYAMTFATFNGANTAPTLNMSSIAQNHAMFHSMPEAARQGYQMMAAQAAQQKM 1140

Query: 1143 NHQTS--EGRNGVVMSGCNDRNT--------GNSSAKPS-ATSGQTLVFGNSARTLNFMT 1202
            N+  S  +G++G +       NT        G +  K S    GQ++ F N     +   
Sbjct: 1141 NYGASLEDGKSGSIGGAATANNTPEEQRKSGGGAIGKTSGGNGGQSIAFSNKQDLADASV 1200

Query: 1203 SSV---PVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIM------------- 1262
            S+V    +   S    +  S   + +GS      QQ L Q  QQH+              
Sbjct: 1201 SAVTSGSIVDSSSRLLNLGSALPQSSGSLPTSHHQQLLQQQQQQHMQRSQSQQPYTTMYL 1260

Query: 1263 --QQQQATMAPRVAPRTKVPT----------NNTLPSSATTKFPYNAPPGFSQPLIQ-CD 1269
              QQ+ AT     A RTK P           N T   + TTKF  NA  GF Q L+Q   
Sbjct: 1261 QKQQRYATSVAASAARTKGPVVSNGSGFPDHNMTTSPAGTTKFA-NANSGFPQNLVQSSS 1320

BLAST of Lag0002401.1 vs. TAIR 10
Match: AT3G63180.1 (TIC-like )

HSP 1 Score: 231.1 bits (588), Expect = 5.0e-60
Identity = 377/1329 (28.37%), Postives = 531/1329 (39.95%), Query Frame = 0

Query: 3    MEKSREARSSSSTVAA-NGSSRRRSRATAFRDLPEEGQVEL-QETVRLRDRGGKRDRDRE 62
            M+K REAR S++ +AA NG ++RR R      + E  + ++ QE   +RDR GK++RD E
Sbjct: 1    MDKPREARRSTTNMAASNGVTKRRQR------ISENSREQMHQEVATVRDRPGKKERDPE 60

Query: 63   FASRSKRRRG---GGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRILSSTTASS 122
              +RSKRRR        E +VEED   ++  +D                           
Sbjct: 61   SFNRSKRRRSERFAPRNERDVEEDSSDESQGDD--------------------------- 120

Query: 123  ASNQNQRRNSLPPRVAKQQWK-AVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAG 182
              + +QR++    R+ +      V D MIG PVPRKARSA +KRSHDC  S  S      
Sbjct: 121  --DYHQRKSFSSGRIPRHAASLMVADEMIGVPVPRKARSACLKRSHDCRTSSGS------ 180

Query: 183  EDIADNHSHRNLSDSPARSSAEVVSPSTSIISAKKMK-PTGPKTRSMKTSKSSSPSAKEG 242
                               S EVVSPS+S +S K+ K   G K+R  K  KSS     E 
Sbjct: 181  ------------------HSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPKSS--GTVED 240

Query: 243  DIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTA 302
            D+EIEIAEVL GLKKQP  SK+           ++ENS                      
Sbjct: 241  DLEIEIAEVLSGLKKQPHSSKR---------GDDSENS---------------------- 300

Query: 303  FQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQK 362
             QKS                                       KG  + PA  +EI    
Sbjct: 301  -QKS------------------------------------SEVKGIKQLPAG-VEI---- 360

Query: 363  LERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDS 422
                      LF+ + + + +          D++    KT L    VAS    D+  E  
Sbjct: 361  --------CGLFTCKARFDSANI--------DKSTNGAKTSLV---VAS----DIVDEKE 420

Query: 423  PPTKSIPVVETQKEE-----KFKFDLMAPPTSPERDG---LADMVLDAKPLSLGIEMGKE 482
            P     P +E  K+E     K    LM PPT P   G   L  +V D       ++  ++
Sbjct: 421  PRVSEGPQLENNKDESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEK 480

Query: 483  TSNKVENEVVGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKP----QLDGNCIIMQ-EQSQ 542
             +  V+NE+   + K+K VD+           +  LD+E P      D N  +   + SQ
Sbjct: 481  IT--VKNEMNPEEVKQKKVDKR---------YWLNLDIEGPNQETDRDSNLRLQNLDWSQ 540

Query: 543  KQQLKGAAGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQ 602
             QQ K A         S+ + LP+ +  WP + +PP G++   +T  PVD  +  S  +Q
Sbjct: 541  PQQAKSA-------PHSSVLPLPVAVASWP-SGVPPQGHVALIQTGKPVDESNGSSKLVQ 600

Query: 603  HPNFILSHPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGM 662
                  S PRPKRC TH+ +ARNI LHQ F KT++L      S  L    L+  +     
Sbjct: 601  GAPISASQPRPKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAG---- 660

Query: 663  ILSNSLGGNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGA 722
              + SL G+   +SLNS  Q     GD + +  N K+ E+ +FA   ++K     Q   A
Sbjct: 661  --NPSLHGSSPILSLNS--QPHVRNGD-NISAPNVKASESGHFASTRQNK----PQPPPA 720

Query: 723  SFGNSMVPGSGFIFPIGQHQAPTAATGNQSGSTKSSNQSTSLFNNPEAGTLVSFPALPAV 782
            S   S+VP   FI+P   H  P       S  TKS + +  L                  
Sbjct: 721  SI--SVVPAPAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGL-----------------A 780

Query: 783  SSNMSYSHPNVVSIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQ 842
            S+N S+   +   + +PYL  + N  Y F  S     S       +Q +P +NGSFY  Q
Sbjct: 781  SANFSHPSSSASEVSSPYLTVIPNNAYSFQLS-----STIRGGTPSQAVPFYNGSFYSPQ 840

Query: 843  LFHPSQIQPAQTQSQHQ--------PSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQS 902
            +F     QP Q QSQ Q         S SH+QPQ         V  N+L   + +Q    
Sbjct: 841  MFQQPP-QPLQRQSQAQRESKASSCSSSSHRQPQ---------VSVNSLSSQANVQ---- 900

Query: 903  TEQNLPMSNHQSRKHESETSGEDATSLPDKRAAPVQKNA-YGQNFILPVQALGFTLMPSA 962
                     H+    + E +G +     D R +  QK   +GQ    PVQ          
Sbjct: 901  --------QHRQMSQKFEVAGVNT----DSRGSHTQKGGPFGQIMAAPVQ---------- 960

Query: 963  TMNNGNAGNNGDKKQSHTQNLKSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAV 1022
                                          Q  +MSFAS   +  P+ LNF+        
Sbjct: 961  -----------------------------PQNFSMSFASIASSAPPATLNFS-------- 978

Query: 1023 FQNLPDMQRAGFQVAPAPQATKQKNHQTSEGRNGVVMSGCNDRN-TGNSSAKPSA-TSGQ 1082
                      G+ ++  P    QKNHQ+SE + G      N  +   N   KP    +G 
Sbjct: 1021 --------SNGYHISTPPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGH 978

Query: 1083 TLVFGNSARTLNFMTSSVPVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQ 1142
            TLVF N +RTLNF++ +    WP P    AA+  N   G  S          +  QH+ Q
Sbjct: 1081 TLVFDNPSRTLNFVSGT----WPPP----AATAIN---GDPS----------VFTQHLTQ 978

Query: 1143 QQQATMAPRVAPRTKVPTNNTLPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRM 1202
            +QQ       + R+K+   ++   S                      S  S   KN    
Sbjct: 1141 RQQQ------SGRSKMMMTHSQADSV---------------------SATSSQWKN---- 978

Query: 1203 VASGVPMTSLHLASSTAVH-KNSTQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSP 1262
                 P TS  L S T+++ K    Q+     GQTQISF     P  +Q +   +   SP
Sbjct: 1201 -----PATSSSLTSCTSLNLKQFQSQQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSP 978

Query: 1263 SSSGKL---RNTSSKTSPS----VAPAQPKQDEGSS-TTGAGQKSSPVCGRNVPSILNTC 1292
            S +G     +  +SK S S    ++P    Q+   +  +G+ QK+SPVCGR VP I+ +C
Sbjct: 1261 SVTGSASHGKPANSKVSNSKALLLSPVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSC 978

BLAST of Lag0002401.1 vs. TAIR 10
Match: AT3G63180.2 (TIC-like )

HSP 1 Score: 225.3 bits (573), Expect = 2.8e-58
Identity = 376/1329 (28.29%), Postives = 530/1329 (39.88%), Query Frame = 0

Query: 3    MEKSREARSSSSTVAA-NGSSRRRSRATAFRDLPEEGQVEL-QETVRLRDRGGKRDRDRE 62
            M+K REAR S++ +AA NG ++RR R      + E  + ++ QE   +RDR GK++RD E
Sbjct: 1    MDKPREARRSTTNMAASNGVTKRRQR------ISENSREQMHQEVATVRDRPGKKERDPE 60

Query: 63   FASRSKRRRG---GGNREEEVEEDEGGDTSAEDIVADGYSDEVEDVGGVSRILSSTTASS 122
              +RSKRRR        E +VEED   ++  +D                           
Sbjct: 61   SFNRSKRRRSERFAPRNERDVEEDSSDESQGDD--------------------------- 120

Query: 123  ASNQNQRRNSLPPRVAKQQWK-AVDDAMIGAPVPRKARSASVKRSHDCTVSGNSGVGGAG 182
              + +QR++    R+ +      V D MIG PVPRKARSA +KRSHDC  S  S      
Sbjct: 121  --DYHQRKSFSSGRIPRHAASLMVADEMIGVPVPRKARSACLKRSHDCRTSSGS------ 180

Query: 183  EDIADNHSHRNLSDSPARSSAEVVSPSTSIISAKKMK-PTGPKTRSMKTSKSSSPSAKEG 242
                               S EVVSPS+S +S K+ K   G K+R  K  KSS     E 
Sbjct: 181  ------------------HSCEVVSPSSSTLSVKERKIVNGSKSRIPKPPKSS--GTVED 240

Query: 243  DIEIEIAEVLFGLKKQPQCSKKQEVNVKPSSNQETENSSVLRDVTKSSVASATANSSQTA 302
            D+EIEIAEVL GLKKQP  SK+           ++ENS                      
Sbjct: 241  DLEIEIAEVLSGLKKQPHSSKR---------GDDSENS---------------------- 300

Query: 303  FQKSVSLQKNGAISDLLLNVAVEKNKVESSSSLVQNDSLDGAAKGESEQPADMMEIYPQK 362
             QKS                                       KG  + PA  +EI    
Sbjct: 301  -QKS------------------------------------SEVKGIKQLPAG-VEI---- 360

Query: 363  LERASDLSMNLFSHERKTEESKPTTVVYTGGDENKGSQKTVLTQEDVASCAKGDVDLEDS 422
                      LF+ + + + +          D++    KT L    VAS    D+  E  
Sbjct: 361  --------CGLFTCKARFDSANI--------DKSTNGAKTSLV---VAS----DIVDEKE 420

Query: 423  PPTKSIPVVETQKEE-----KFKFDLMAPPTSPERDG---LADMVLDAKPLSLGIEMGKE 482
            P     P +E  K+E     K    LM PPT P   G   L  +V D       ++  ++
Sbjct: 421  PRVSEGPQLENNKDESHCISKLDIHLMVPPTLPSSSGRVSLLPLVSDYCKRDSALKSKEK 480

Query: 483  TSNKVENEVVGFKEKEKVVDEDKMVTSGKNIEFFKLDLEKP----QLDGNCIIMQ-EQSQ 542
             +  V+NE+   + K+K VD+           +  LD+E P      D N  +   + SQ
Sbjct: 481  IT--VKNEMNPEEVKQKKVDKR---------YWLNLDIEGPNQETDRDSNLRLQNLDWSQ 540

Query: 543  KQQLKGAAGTVEKNESSTSVRLPIILGGWPTTEIPPVGYMPPFRTVLPVDSVDKPSTKLQ 602
             QQ K A         S+ + LP+ +  WP + +PP G++   +T  PVD  +  S  +Q
Sbjct: 541  PQQAKSA-------PHSSVLPLPVAVASWP-SGVPPQGHVALIQTGKPVDESNGSSKLVQ 600

Query: 603  HPNFILSHPRPKRCLTHYDVARNIYLHQQFTKTSYLHPTGDASASLAEVKLKNMSSKEGM 662
                    PRPKRC TH+ +ARNI LHQ F KT++L      S  L    L+  +     
Sbjct: 601  --------PRPKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAG---- 660

Query: 663  ILSNSLGGNHLEVSLNSVQQRDQTEGDFSGNVVNDKSPEAANFADMAKSKQLVFQQRQGA 722
              + SL G+   +SLNS  Q     GD + +  N K+ E+ +FA   ++K     Q   A
Sbjct: 661  --NPSLHGSSPILSLNS--QPHVRNGD-NISAPNVKASESGHFASTRQNK----PQPPPA 720

Query: 723  SFGNSMVPGSGFIFPIGQHQAPTAATGNQSGSTKSSNQSTSLFNNPEAGTLVSFPALPAV 782
            S   S+VP   FI+P   H  P       S  TKS + +  L                  
Sbjct: 721  SI--SVVPAPAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGL-----------------A 780

Query: 783  SSNMSYSHPNVVSIEAPYLAKLQNKGYPFTFSTPAGTSATYRANNAQPLPLFNGSFYPSQ 842
            S+N S+   +   + +PYL  + N  Y F  S     S       +Q +P +NGSFY  Q
Sbjct: 781  SANFSHPSSSASEVSSPYLTVIPNNAYSFQLS-----STIRGGTPSQAVPFYNGSFYSPQ 840

Query: 843  LFHPSQIQPAQTQSQHQ--------PSGSHKQPQSQQQQWGVHVPANNLVPSSGMQVKQS 902
            +F     QP Q QSQ Q         S SH+QPQ         V  N+L   + +Q    
Sbjct: 841  MFQQPP-QPLQRQSQAQRESKASSCSSSSHRQPQ---------VSVNSLSSQANVQ---- 900

Query: 903  TEQNLPMSNHQSRKHESETSGEDATSLPDKRAAPVQKNA-YGQNFILPVQALGFTLMPSA 962
                     H+    + E +G +     D R +  QK   +GQ    PVQ          
Sbjct: 901  --------QHRQMSQKFEVAGVNT----DSRGSHTQKGGPFGQIMAAPVQ---------- 960

Query: 963  TMNNGNAGNNGDKKQSHTQNLKSTVALVPSQGLTMSFASYNGNGTPSNLNFTPIAQNTAV 1022
                                          Q  +MSFAS   +  P+ LNF+        
Sbjct: 961  -----------------------------PQNFSMSFASIASSAPPATLNFS-------- 970

Query: 1023 FQNLPDMQRAGFQVAPAPQATKQKNHQTSEGRNGVVMSGCNDRN-TGNSSAKPSA-TSGQ 1082
                      G+ ++  P    QKNHQ+SE + G      N  +   N   KP    +G 
Sbjct: 1021 --------SNGYHISTPPGVAHQKNHQSSEAKTGGGSCSSNAEDPKKNLQGKPQGMMNGH 970

Query: 1083 TLVFGNSARTLNFMTSSVPVNWPSPSTKSAASTTNRPAGSSSGHQQQQPLLQLPQQHIMQ 1142
            TLVF N +RTLNF++ +    WP P    AA+  N   G  S          +  QH+ Q
Sbjct: 1081 TLVFDNPSRTLNFVSGT----WPPP----AATAIN---GDPS----------VFTQHLTQ 970

Query: 1143 QQQATMAPRVAPRTKVPTNNTLPSSATTKFPYNAPPGFSQPLIQCDNSIQSPAQKNSGRM 1202
            +QQ       + R+K+   ++   S                      S  S   KN    
Sbjct: 1141 RQQQ------SGRSKMMMTHSQADSV---------------------SATSSQWKN---- 970

Query: 1203 VASGVPMTSLHLASSTAVH-KNSTQQKGSTPQGQTQISFGGDFKPAFTQMQHIPTSSHSP 1262
                 P TS  L S T+++ K    Q+     GQTQISF     P  +Q +   +   SP
Sbjct: 1201 -----PATSSSLTSCTSLNLKQFQSQQQIRTHGQTQISFAAPTNPQPSQGKQGRSGGSSP 970

Query: 1263 SSSGKL---RNTSSKTSPS----VAPAQPKQDEGSS-TTGAGQKSSPVCGRNVPSILNTC 1292
            S +G     +  +SK S S    ++P    Q+   +  +G+ QK+SPVCGR VP I+ +C
Sbjct: 1261 SVTGSASHGKPANSKVSNSKALLLSPVPLSQEHTENPASGSTQKNSPVCGRTVPPIITSC 970