Lag0001594.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0001594.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily
Locationchr4: 33253333 .. 33257603 (-)
Sequence length1110
RNA-Seq ExpressionLag0001594.1
SyntenyLag0001594.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTTGGGTGTGTGTGCGGAGCGTGACGCGGAAATCACGTGTGGGTGTGAGTGCGGAGCGTGACGCGGAAATCACGTGTGAGTGGCGCTGCGCATGGCGCGTTATAATACATGTGTTGGGTGGGTTTGCGGAGCGTGACGCGGAAATCACGTGTGGGATGTGTGTGCGGAGCGTGACGCGGAAATCACGTGAGTGTGCGGGCATGGCGCGGAAACCATGTGGGGGTGCATAGCATGATGCATGGATTCATGTTGTCGTCCCATTTTTGCATGAGCATGTGTTGCCATTGTTAATTTCGTGTTTAAGAGCATGTGGGTTGTTTATCGCTTTATAAGCATGATGTGTGTGGTTAGCTTGTTGTGGTGGTTGTTTGTTGTTTGGTGGCTCACCGGGTGTGAGCTACTTACCAGTACGGTGGTTGTACTGATACCCCTCCCCTTTATTCCCCCAACAATTTCAGATTATGCAGGTGATGAGCTGTATGTGCCCGGTGATGTAGACGAGGAGGTCCACGAGGGGTGACCACAAGGTTCCATAGTTTGTTTATATGTTTTTTTTTCTTTCTTTCTCTGTTAGAAAGGACAGTTTTCAATTAATTGTTGATAGGTAGGTCTGTTTAGCTTTGTAGCTAGGTGGGCGAAATTTTTGTTCCTGTTATTGAGCATGGTTAGATTTCATGTTTCTTGTTAGGCTGTAAATATGTTGTAGCTTAATAAAGTCGATCTGTTTTAGGAAGTTAAGGTTATGCTTGATCTGGTAGAGTTAGGTTCTGTTGTTGGTTGGAGATGCGAGATCTGACTCGTATCCTGCTGCAGTTGTTCTGTGAAGTTGTGGAGTGGATCGAATCAATTTAGAAATGTTGTTGAGTTTAACAGGTGTTCTAAAATTTTGGGTTTGTCCTATGTACGTCGTTATGCTGCCGAAATTTCCGGAGCTCTCGGTTGGTTTGAATAGGAGCTTAGTAATGTCACTAGGTTAGTTTATATTAAAACCTGGGGCGTTACAATAAGTGATGTTGACAATAGGGATAAAACCAAGGTTCTTTTCAAATATAGAAGCCTAAATTTCATCCTCATTTATATGCTAGCTACTTAGCCAACAATATTGCCTAATTTACATCTTTCCTTTCTAATATATATGTCTTTAATTCGCCTAATTTACATCTTTCCTTTCTAATATATGTCTTTAATTTGAACCGTCATTACTTACTTTGTAAAGTCTACATATCGCAGTCTTTATAAAAAAAAAAAAAAAGTCTACATATCACACGCTCATGATATGCCTTTCCTTATAAGTTGTAAAAACGTTCTCTACAAAAATAATTCTCTTATCTACTCATAGATTATGGGCCAACCCCACTTTTTTTTAGTACAAAGATTGTGGTCAACTAAAACATTCAAGCCAAAGAGCCAAGGGGCTGACCCCTTAGAGGTCGACCACAATAATAGTAGGTCGAACCCCTGAAGGGTAGGGCAAAGAGCCAAATGGTTGACTCCTTAGGGGTCGATCACGACACAAAAGGACCAAACCCTTGAAGGGTAGGCCAAAGAGCCAAAGGGTTGACCCTTGATGGGTAGGCCACGATACGAGTAGGCCAAACCCTTGAAGGTTAGGCCAAAGAGCCAAAGGACAGATCCCTTAGGGGTCGACCACGAATCGAGTAAGTTAGTTAGGTTAGGAAGCCACCCCATGTGGGATCGACCCTGGCCCAAAGAGCCAAAACGTTAACTTCATGTAGGGTCGACCCCAACGTGAAAAACTTGACCATGAGGGAGTCAGCCCTAAGTCGAAAGAGACTAGTCCCAAGCAGGATCAGCTATGGTAGAGATAAGATGACCCCTTGAAGAAACCAAGGCATGCAAATCTAGGCAATAATGCCTAAGCGAAGGCCAAGGATACATCCATTTCTCAAACTCCAAGAGATAAGTAAACTCTCAACCCAGAGCTAAGTTAGAAAGGTACGACCAAGACATGCTACCTAAGCTCCAGCTAGAGAATGGGGGAATCTGTTGAGGGTAGATTTTCAGGTGGCTCCATTAGGACTACAACTCCAAGAAATTGCTCAAGAGGTTAAAAGAAACTCAAGAGATTCGTTAAAAACCCAAGACCCCAGAAGAGCTAGCAAGGCCAAAAGATCGACCCTTAAAGGGGTTGGCTAAGAGGCCCAAAAGCCATAAAGGACAACTTCGAAGGAACGACCCAAACCCCCCAGACCACAAGTCCGACCCTCCATGAGGCTGACCCCCCACTGCCACCAAGGTTGACCCCCATGGGGGTCGGCCAAGAGCCATAGAGGTCAGCCACAACCCATGAGGCCGACCCTCAAGGGTTATGAGGGTCAGATGTTAGACGCTTAAGGGTCCTACAAACAAGCTAAGGGTCAAGGTCAACGACCGTTACAGTGGACCAAGTCCAAGGACAGTTACAGACTCGTAACTGTCAATTAACAGGATCCGAGAATTAAGGCGGACATAAATGTCAAACGTTATTCTACTTGGTATTATAAATAGAACGTTCTGGAAAAGGGGAAGGTACATCCAACATACACATTTGTCTACTACAGTCTAAGCTCTCTAAGTACTCTCGGAACCATATTCTAACTTGAGCGTCGAAGTGTATGTGGCTTAGCACCGCACCGGTGCTCAGCTCCCTTTTGTTGCAAACACACGATTATTCCCCTTTAATACAAATTTATCGTTGACCCAGCGATACAAAATCTCTTCACATTGCGACCATTTATAACTTTTTGACTAGTTGTGTAGGAAACTGCCATTGGTGAAATGGGAAAATTATTCTCTTACCCATTGGTTTTCACACCAAAAAAAAAAAAAAAAACAAAAAAGTCTACTTTGTTTGCAAGCTATTATGGTATGTGAATGAAAATGAGATTTCTTAGTTAACCAAAACAAATGTTTAAGATTCTCATCCTCATTGTTATTGCCTAATATAAATACAAGATTAATTTCCATTTTACATTCAATATTAGCTTGTCCCTTGCACCTTACCATGGCTTTTCGTCTACTCGTGTTCTATATAGTTATCTTACTAGTTGTCAACAATCATTTTGTTGAATATTCTTATAGCTTTGGTTTTAACTTTACAGTATCTTTAGATGGCTCGGGAAATTTTTCTAGAGTTAACGACGCTATTGCAGCAGCGCCTAACTTCAGCACCACTAGATTTTACATTCACGTGAAACCTGGAACTTATCATGAGCATGTTGAAGTTCCAGTTCAAAAGACATTATTGCTTTGATTGGAGATGATCCTTCTACGACTCTCATCGTTGACAATCGAAGTAATCACACTGGGTTTTCCACTCCAACGTCTGCAACCCTCAGTAAGCTAAGCTACCCTTAACTTTTTCCCCAACAAATAGTAAATTACACAGCATGAATGGTGGTCTATTGAACATAAATTTACTTTCTTTTTTTTTTACTTTTTTGAACAAAATTTACTTTTTTTTTACTTTGTTGAACAAAAATTTACTTTGTTTTGTTCTCCCCAAAAGTTTATATTCCCCCAAAAGTTTATATTCCATTCAACAAACTTTGAAATAAATGTTTTGTTCGTAGCTGTGAATGGTCAAAACTTCATGGCTAAGTTCTTGACTTTTCAAAACTCTGCTGGAGCAGACAAAGGTCAAGCGGTAGCCGTTTTTGATACAGCTAATCACACAGCGTACTATAAATGTGTGTTTTTGGGTTATCAAGATACCCTTTATGCAGGAGCATTGCCCCAATTCTTCAAGAAATGCGACATATATGGGACTATTGACTTCATTTTTGGAGATGGGCTTGCAGTATTCCAGGACTGTAATTTATACGCTCGATTAGCGGACGCTCGTAGTACAGTCACTGCACAATCCAAAGCCAACAAAAACGACTCGAGCGGCTTTGTCTTCCAGAATTGCAATGTGACAGTGTCTCCAGAGATAACGACATCAACATCGTCAAGCAAGGACAATGTTACTTTGTTTCTTGGACGACCATGGAGAGACTATTCGACGGTCGTTTTCATGGAGTCATTTCTTGACGGCGTAGTTCCACCAGAAGGATGGTTAGATTGGTCGAGTACACCCTTGAATTTACTATTTTATGCTGAATATAACAACAGCGGTCCAGGAGCTGACACTTCTCGAAGGGTGACTTGGCCTGGTTACCATGTACTACACAATTCAATGGAGGTTGACAAGTTTACTGTTGGAAACTTCATCGATGGGAATCAATGGCTACCGGAAACTGGCATACCCTTTAGGAGTGGATTCTAA

mRNA sequence

ATGTGTTGGGTGTGTGTGCGGAGCGTGACGCGGAAATCACGTGTGGGTGTGAGTGCGGAGCGTGACGCGGAAATCACGTGTGAGTGGCGCTGCGCATGGCGCGTTATAATACATGTGTTGGGTGGGTTTGCGGAGCGTGACGCGGAAATCACGTGTGGGATGTGTGTGCGGAGCGTGACGCGGAAATCACATTATGCAGGTGATGAGCTGTATGTGCCCGGTGATGTAGACGAGGAGGTCCACGAGGGTTGTTCTGTGAAGTTGTGGAGTGGATCGAATCAATTTAGAAATGTTGTTGAGTTTAACAGTTCAAAAGACATTATTGCTTTGATTGGAGATGATCCTTCTACGACTCTCATCGTTGACAATCGAAGTAATCACACTGGGTTTTCCACTCCAACGTCTGCAACCCTCACTGTGAATGGTCAAAACTTCATGGCTAAGTTCTTGACTTTTCAAAACTCTGCTGGAGCAGACAAAGGTCAAGCGGTAGCCGTTTTTGATACAGCTAATCACACAGCGTACTATAAATGTGTGTTTTTGGGTTATCAAGATACCCTTTATGCAGGAGCATTGCCCCAATTCTTCAAGAAATGCGACATATATGGGACTATTGACTTCATTTTTGGAGATGGGCTTGCAGTATTCCAGGACTGTAATTTATACGCTCGATTAGCGGACGCTCGTAGTACAGTCACTGCACAATCCAAAGCCAACAAAAACGACTCGAGCGGCTTTGTCTTCCAGAATTGCAATGTGACAGTGTCTCCAGAGATAACGACATCAACATCGTCAAGCAAGGACAATGTTACTTTGTTTCTTGGACGACCATGGAGAGACTATTCGACGGTCGTTTTCATGGAGTCATTTCTTGACGGCGTAGTTCCACCAGAAGGATGGTTAGATTGGTCGAGTACACCCTTGAATTTACTATTTTATGCTGAATATAACAACAGCGGTCCAGGAGCTGACACTTCTCGAAGGGTGACTTGGCCTGGTTACCATGTACTACACAATTCAATGGAGGTTGACAAGTTTACTGTTGGAAACTTCATCGATGGGAATCAATGGCTACCGGAAACTGGCATACCCTTTAGGAGTGGATTCTAA

Coding sequence (CDS)

ATGTGTTGGGTGTGTGTGCGGAGCGTGACGCGGAAATCACGTGTGGGTGTGAGTGCGGAGCGTGACGCGGAAATCACGTGTGAGTGGCGCTGCGCATGGCGCGTTATAATACATGTGTTGGGTGGGTTTGCGGAGCGTGACGCGGAAATCACGTGTGGGATGTGTGTGCGGAGCGTGACGCGGAAATCACATTATGCAGGTGATGAGCTGTATGTGCCCGGTGATGTAGACGAGGAGGTCCACGAGGGTTGTTCTGTGAAGTTGTGGAGTGGATCGAATCAATTTAGAAATGTTGTTGAGTTTAACAGTTCAAAAGACATTATTGCTTTGATTGGAGATGATCCTTCTACGACTCTCATCGTTGACAATCGAAGTAATCACACTGGGTTTTCCACTCCAACGTCTGCAACCCTCACTGTGAATGGTCAAAACTTCATGGCTAAGTTCTTGACTTTTCAAAACTCTGCTGGAGCAGACAAAGGTCAAGCGGTAGCCGTTTTTGATACAGCTAATCACACAGCGTACTATAAATGTGTGTTTTTGGGTTATCAAGATACCCTTTATGCAGGAGCATTGCCCCAATTCTTCAAGAAATGCGACATATATGGGACTATTGACTTCATTTTTGGAGATGGGCTTGCAGTATTCCAGGACTGTAATTTATACGCTCGATTAGCGGACGCTCGTAGTACAGTCACTGCACAATCCAAAGCCAACAAAAACGACTCGAGCGGCTTTGTCTTCCAGAATTGCAATGTGACAGTGTCTCCAGAGATAACGACATCAACATCGTCAAGCAAGGACAATGTTACTTTGTTTCTTGGACGACCATGGAGAGACTATTCGACGGTCGTTTTCATGGAGTCATTTCTTGACGGCGTAGTTCCACCAGAAGGATGGTTAGATTGGTCGAGTACACCCTTGAATTTACTATTTTATGCTGAATATAACAACAGCGGTCCAGGAGCTGACACTTCTCGAAGGGTGACTTGGCCTGGTTACCATGTACTACACAATTCAATGGAGGTTGACAAGTTTACTGTTGGAAACTTCATCGATGGGAATCAATGGCTACCGGAAACTGGCATACCCTTTAGGAGTGGATTCTAA

Protein sequence

MCWVCVRSVTRKSRVGVSAERDAEITCEWRCAWRVIIHVLGGFAERDAEITCGMCVRSVTRKSHYAGDELYVPGDVDEEVHEGCSVKLWSGSNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF
Homology
BLAST of Lag0001594.1 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 418.7 bits (1075), Expect = 5.2e-113
Identity = 199/274 (72.63%), Postives = 226/274 (82.48%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++  VE    K  IALIG+D STT+IVDNRSN +GFST T+ATL V GQNFMA+FLTF+N
Sbjct: 68  YKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFEN 127

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           SA   +GQA+AV D A HTAYYKCVFL YQDTLYAGA+PQFFK+CDIYG IDFIFG+GL 
Sbjct: 128 SAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLV 187

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YAR+      VTAQSK    D SGF+FQNCNVTVSPEI    +SSKDNVT+FL
Sbjct: 188 VFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEI----ASSKDNVTMFL 247

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWRDYS V+FMESFLD VV P+GWL+W   P+NLLFYAEYNN GPGADTS+RV WPGY
Sbjct: 248 GRPWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGY 307

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           HVLHN+MEV KFTV NFI+G +WLPETGIPFR+G
Sbjct: 308 HVLHNAMEVTKFTVENFINGTKWLPETGIPFRAG 337

BLAST of Lag0001594.1 vs. NCBI nr
Match: XP_011654361.2 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 411.8 bits (1057), Expect = 6.4e-111
Identity = 194/275 (70.55%), Postives = 229/275 (83.27%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++ ++E    K  IALIGDD STT+IV+NRSN TG ST +SATLTVNG NFMA+FLTFQN
Sbjct: 67  YQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQN 126

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           +AG ++GQA+A+ D A HTAYYKCVFLGYQDTLYAGALPQFFK+CDIYG++DFIFG+GL 
Sbjct: 127 TAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLV 186

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YARL D + TVTAQSK + N  SGF+FQNCNVTVSPEI    + SKDNVT+FL
Sbjct: 187 VFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEI----APSKDNVTVFL 246

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWR YS VVF++SFLD VV P+GWL+W   P NLL+YAEYNNSG GA+TS+RV WPGY
Sbjct: 247 GRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGY 306

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           H L+N+ EV  FTV  FI+G QWLP+TGIPFR+GF
Sbjct: 307 HALNNAKEVATFTVETFINGTQWLPQTGIPFRAGF 337

BLAST of Lag0001594.1 vs. NCBI nr
Match: XP_022150325.1 (probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 3.2e-110
Identity = 195/270 (72.22%), Postives = 224/270 (82.96%), Query Frame = 0

Query: 99  VEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGA 158
           VE  + K  IALIGDD STT+IVDNRSN TGFST TSATLTV+G+NFMA+ LTFQNSAG 
Sbjct: 72  VEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMAQSLTFQNSAGP 131

Query: 159 DKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQD 218
           DKGQAVAV D A HT YYKCVFLGYQDTL+AGA+PQFFK+CDIYG++DFIFG+GLA+FQD
Sbjct: 132 DKGQAVAVLDQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDFIFGNGLAIFQD 191

Query: 219 CNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGRPW 278
           CN+YARL+D   TVTAQSK N ++ SGF+FQ CNVTVSPEI     SSK+ VT+FLGRPW
Sbjct: 192 CNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIV----SSKNKVTVFLGRPW 251

Query: 279 RDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGYHVLH 338
           R YS V F+ESFLD VV P+GWL+W  TP+NLLFYAEY NSG G++TS RV WPG+H+  
Sbjct: 252 RTYSMVAFIESFLDDVVQPKGWLEWPGTPVNLLFYAEYKNSGTGSETSNRVDWPGFHLFS 311

Query: 339 NSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
            + EV  FTV NFIDG QWLPETGIPFR+G
Sbjct: 312 YAAEVANFTVENFIDGTQWLPETGIPFRTG 337

BLAST of Lag0001594.1 vs. NCBI nr
Match: XP_023546999.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409.1 bits (1050), Expect = 4.1e-110
Identity = 193/275 (70.18%), Postives = 228/275 (82.91%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++ ++E   SK  IALIGDD STT+IVD+RSN TG +T TSATLTV G NFMA+ LTFQN
Sbjct: 64  YKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQN 123

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           SAG  + QAVAV D A HTAYYKCVFLGYQDTLYAG LPQFF++CDIYG+IDFIFG+G+ 
Sbjct: 124 SAGPKEHQAVAVLDQAKHTAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGNGVT 183

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+Y R   +++TVTAQSKAN +D SGFVFQNC VTVSPEI    +SSKD V L+L
Sbjct: 184 VFQDCNIYVREFGSKTTVTAQSKANIDDQSGFVFQNCKVTVSPEI----ASSKDKVLLYL 243

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPW++YSTVVF+ESFLD VV P+GWL W   P++ LF+AEYNN+GPGADTSRRV WPGY
Sbjct: 244 GRPWKEYSTVVFIESFLDSVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGY 303

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           H+LH + +V +FTV NFI G+QWLPETGIPFR+GF
Sbjct: 304 HILHTATQVTRFTVENFIGGSQWLPETGIPFRTGF 334

BLAST of Lag0001594.1 vs. NCBI nr
Match: XP_031741746.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE8648510.1 hypothetical protein Csa_009140 [Cucumis sativus])

HSP 1 Score: 407.9 bits (1047), Expect = 9.2e-110
Identity = 194/275 (70.55%), Postives = 229/275 (83.27%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++ ++E    K  IALIGDD STT+IV+NRSN TG ST +SATLTVNG NFMA+FLTFQN
Sbjct: 40  YKEIIEVPYEKTCIALIGDDASTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQN 99

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           +AG ++GQA+AV D A HTAYYKCVFLGYQDTLYAGALPQFFK+CDIYG++DFIFG+GL 
Sbjct: 100 TAGPNEGQAIAVLDQAKHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLV 159

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YARL D + TVTAQSK + +  SGF+FQNC VTVSPEI    +SSKD VT+FL
Sbjct: 160 VFQDCNIYARLFDVQITVTAQSKPSLSALSGFIFQNCKVTVSPEI----ASSKDKVTVFL 219

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWR YS VVF++SFLD VV P+GWL+W   P NLL+YAEYNNSG GA+TS+RV WPGY
Sbjct: 220 GRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGY 279

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           HVL+N+ EV  FTV  FI+G QWLP+TGIPFR+GF
Sbjct: 280 HVLNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 310

BLAST of Lag0001594.1 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.0e-67
Identity = 123/263 (46.77%), Postives = 176/263 (66.92%), Query Frame = 0

Query: 108 IALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGADKGQAVAVF 167
           I  +GD    T+I  ++S   G +T  SAT+ V G NF+A+ +T +N+AG +  QAVA+ 
Sbjct: 251 IMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALR 310

Query: 168 DTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQDCNLYARLAD 227
             ++ + +Y+C F GYQDTLY  +  QF+++CDIYGT+DFIFG+   V Q+CN++AR   
Sbjct: 311 SGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPP 370

Query: 228 AR-STVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGRPWRDYSTVVF 287
            R +T+TAQ + + N S+G +  NC VT + ++    SS    V  FLGRPW+ YS  V+
Sbjct: 371 NRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSS----VKTFLGRPWKQYSRTVY 430

Query: 288 MESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSRRVTWPGYHVLHNSMEVDK 347
           +++FLD ++ P GW++WS    LN L+YAEY N+GPG+ T+ RV W GYHVL +  +V +
Sbjct: 431 IKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQ 490

Query: 348 FTVGNFIDGNQWLPETGIPFRSG 369
           FTVGNFI GN WLP T +PF SG
Sbjct: 491 FTVGNFIAGNSWLPATNVPFTSG 509

BLAST of Lag0001594.1 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 8.5e-66
Identity = 125/284 (44.01%), Postives = 185/284 (65.14%), Query Frame = 0

Query: 88  LWSGSNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMA 147
           +++ +  +   V  ++ K  I LIGD  + T+I  N S   G++T  S+T  V G  F+A
Sbjct: 341 IYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVA 400

Query: 148 KFLTFQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDF 207
             +TF+N+AG +K QAVAV + A+ + +Y+C F GYQDTLY  +L QF+++CDIYGTIDF
Sbjct: 401 VDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDF 460

Query: 208 IFGDGLAVFQDCNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSS 267
           IFG+  A+FQ+CN+YAR  +A+ ++ VTA  + + N  +G    NC +  +P++     S
Sbjct: 461 IFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKS 520

Query: 268 SKDNVTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWS-STPLNLLFYAEYNNSGPGAD 327
           +      FLGRPW+ YS  V+++S++  VV P GWL+W+ +T L+ + Y EY+N GPGAD
Sbjct: 521 T----MTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGAD 580

Query: 328 TSRRVTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           TS+RV W GY +L N ++   FTV NF  G+ WLP+T IPF  G
Sbjct: 581 TSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQTDIPFYGG 619

BLAST of Lag0001594.1 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 250.8 bits (639), Expect = 2.5e-65
Identity = 121/280 (43.21%), Postives = 182/280 (65.00%), Query Frame = 0

Query: 92  SNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLT 151
           S  +   V    +K  + +IGD  + T++  NR+   G++T  SAT  V   NF+A  +T
Sbjct: 296 SGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMT 355

Query: 152 FQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGD 211
           F+N+AG +K QAVA+  +A+ + +Y C F  YQDTLY  +L QF+++CDIYGT+DFIFG+
Sbjct: 356 FRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGN 415

Query: 212 GLAVFQDCNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDN 271
              VFQDCNLY R  + +  + +TAQ + + N ++G    NC +  + ++     SS   
Sbjct: 416 AAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV----SSNYT 475

Query: 272 VTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSRR 331
           V  +LGRPW++YS  VFM+S++D VV P GW +W+    L+ L+YAEYNN+G G+ T+ R
Sbjct: 476 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 535

Query: 332 VTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           V WPGYHV+ NS + + FTV NF+ G+ W+ ++G+P+ SG
Sbjct: 536 VVWPGYHVI-NSTDANNFTVENFLLGDGWMVQSGVPYISG 570

BLAST of Lag0001594.1 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.5e-65
Identity = 121/273 (44.32%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 99  VEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGA 158
           VE    K  I ++GD    T+I  NRS   G++T  SAT  V+G+ F+A+ +TFQN+AG 
Sbjct: 256 VEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGP 315

Query: 159 DKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQD 218
           +K QAVA+    +   +Y+C   GYQDTLYA ++ QFF++C I GT+DFIFGD  AVFQ 
Sbjct: 316 EKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQS 375

Query: 219 CNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGR 278
           C + A+  L + ++++TAQ + + N+ +GF  Q  N+    ++  + +++      +LGR
Sbjct: 376 CQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT----ATYLGR 435

Query: 279 PWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSRRVTWPGYH 338
           PW+ YS  VFM++++   + P GWL+W+    L+ L+Y EY NSGPGA   RRV WPGYH
Sbjct: 436 PWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYH 495

Query: 339 VLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           VL+ S E + FTV   I GN WLP TGI F +G
Sbjct: 496 VLNTSAEANNFTVSQLIQGNLWLPSTGITFIAG 524

BLAST of Lag0001594.1 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.5e-65
Identity = 127/282 (45.04%), Postives = 174/282 (61.70%), Query Frame = 0

Query: 91  GSNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFL 150
           G   F N VE    K +I  IGD    T+I  NRS   G+ST  + T+ V G+ ++AK +
Sbjct: 256 GGEYFEN-VELPKKKTMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDI 315

Query: 151 TFQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFG 210
           +F NSAG  K QAVA    ++H+A+Y+C F GYQDTLY  +  QF+++CDIYGTIDFIFG
Sbjct: 316 SFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFG 375

Query: 211 DGLAVFQDCNLYARLADA--RSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKD 270
           +   VFQ+ +LYAR  +   +   TAQS+   +  +G    NC +  +P++       K+
Sbjct: 376 NAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLI----PVKE 435

Query: 271 NVTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSR 330
           N   +LGRPWR YS  V ++SF+D ++ P GWL+      L  L+Y EY N GPGA+ ++
Sbjct: 436 NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAK 495

Query: 331 RVTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           RVTWPG+  + N  E  +FTVG FIDG+ WL  TGIPF  GF
Sbjct: 496 RVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLGF 532

BLAST of Lag0001594.1 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.5e-113
Identity = 199/274 (72.63%), Postives = 226/274 (82.48%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++  VE    K  IALIG+D STT+IVDNRSN +GFST T+ATL V GQNFMA+FLTF+N
Sbjct: 68  YKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFEN 127

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           SA   +GQA+AV D A HTAYYKCVFL YQDTLYAGA+PQFFK+CDIYG IDFIFG+GL 
Sbjct: 128 SAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLV 187

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YAR+      VTAQSK    D SGF+FQNCNVTVSPEI    +SSKDNVT+FL
Sbjct: 188 VFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEI----ASSKDNVTMFL 247

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWRDYS V+FMESFLD VV P+GWL+W   P+NLLFYAEYNN GPGADTS+RV WPGY
Sbjct: 248 GRPWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDWPGY 307

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           HVLHN+MEV KFTV NFI+G +WLPETGIPFR+G
Sbjct: 308 HVLHNAMEVTKFTVENFINGTKWLPETGIPFRAG 337

BLAST of Lag0001594.1 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.5e-110
Identity = 195/270 (72.22%), Postives = 224/270 (82.96%), Query Frame = 0

Query: 99  VEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGA 158
           VE  + K  IALIGDD STT+IVDNRSN TGFST TSATLTV+G+NFMA+ LTFQNSAG 
Sbjct: 72  VEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMAQSLTFQNSAGP 131

Query: 159 DKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQD 218
           DKGQAVAV D A HT YYKCVFLGYQDTL+AGA+PQFFK+CDIYG++DFIFG+GLA+FQD
Sbjct: 132 DKGQAVAVLDQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDFIFGNGLAIFQD 191

Query: 219 CNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGRPW 278
           CN+YARL+D   TVTAQSK N ++ SGF+FQ CNVTVSPEI     SSK+ VT+FLGRPW
Sbjct: 192 CNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIV----SSKNKVTVFLGRPW 251

Query: 279 RDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGYHVLH 338
           R YS V F+ESFLD VV P+GWL+W  TP+NLLFYAEY NSG G++TS RV WPG+H+  
Sbjct: 252 RTYSMVAFIESFLDDVVQPKGWLEWPGTPVNLLFYAEYKNSGTGSETSNRVDWPGFHLFS 311

Query: 339 NSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
            + EV  FTV NFIDG QWLPETGIPFR+G
Sbjct: 312 YAAEVANFTVENFIDGTQWLPETGIPFRTG 337

BLAST of Lag0001594.1 vs. ExPASy TrEMBL
Match: A0A0A0L1M3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 4.5e-110
Identity = 193/275 (70.18%), Postives = 228/275 (82.91%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++ ++E    K  IALIGDD STT+IV+NRSN TG ST +SATLTVNG NFMA+FLTFQN
Sbjct: 60  YQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQN 119

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           +AG ++GQA+A+ D A HTAYYKCVFLGYQDTLYAGALPQFFK+CDIYG++DFIFG+GL 
Sbjct: 120 TAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLV 179

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YARL D + TVTAQSK + N  SGF+FQNC VTVSPEI    +SSKD VT+FL
Sbjct: 180 VFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEI----ASSKDKVTVFL 239

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWR YS VVF++SFLD VV P+GWL+W   P NLL+YAEYNNSG GA+TS+RV WPGY
Sbjct: 240 GRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGY 299

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           H L+N+ EV  FTV  FI+G QWLP+TGIPFR+GF
Sbjct: 300 HALNNAKEVATFTVEIFINGTQWLPQTGIPFRAGF 330

BLAST of Lag0001594.1 vs. ExPASy TrEMBL
Match: A0A6J1KJG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111493749 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.3e-109
Identity = 192/275 (69.82%), Postives = 228/275 (82.91%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           +  V+E   SK  IALIG+D STT+IVD+RSN TG +T TSATLTV G NFMA+ LTFQN
Sbjct: 64  YNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLTFQN 123

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           SAG  + QAVAV D A HT+YYKCVFLGYQDTLYAG LPQFFK+CDIYG+IDFIFG+G++
Sbjct: 124 SAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGNGVS 183

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+Y R    ++TVTAQSKAN +D SGFVFQNC VTV+PEI    +SSKD V L+L
Sbjct: 184 VFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEI----ASSKDKVLLYL 243

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWR+YSTVVF+ESFLD VV P+GWL W + P++ LF+AEYNN+GPGADTSRRV WPGY
Sbjct: 244 GRPWREYSTVVFIESFLDSVVQPKGWLGWPNVPVDQLFFAEYNNTGPGADTSRRVDWPGY 303

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           H+LH + +V +FTV NFI G++WLPETGIPFR+GF
Sbjct: 304 HILHTATQVTRFTVENFIGGSRWLPETGIPFRTGF 334

BLAST of Lag0001594.1 vs. ExPASy TrEMBL
Match: A0A5D3C2R6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.7e-109
Identity = 191/275 (69.45%), Postives = 230/275 (83.64%), Query Frame = 0

Query: 95  FRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQN 154
           ++ ++E  S K  IALIGDD STT+IV+NRSN TG ST +SATLTV G+NFMA+FLTFQN
Sbjct: 47  YQEIIEVPSEKTFIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVYGENFMARFLTFQN 106

Query: 155 SAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLA 214
           SAG ++GQA+AV D A HTAYY+CVFLGYQDTLYAG++PQFFK+CDIYG++DFIFG+GL 
Sbjct: 107 SAGPNEGQAIAVLDQAKHTAYYRCVFLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLV 166

Query: 215 VFQDCNLYARLADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 274
           VFQDCN+YARL D + TVTAQSK + +  SGF+FQNCNVTVSPEI    +SSKD VT+FL
Sbjct: 167 VFQDCNIYARLFDVQITVTAQSKQSLSAPSGFIFQNCNVTVSPEI----ASSKDKVTVFL 226

Query: 275 GRPWRDYSTVVFMESFLDGVVPPEGWLDWSSTPLNLLFYAEYNNSGPGADTSRRVTWPGY 334
           GRPWR YS VVF++SFLD VV P+GWL+W   P N+L+YAEYNNSG GA+TS+RV WPGY
Sbjct: 227 GRPWRMYSMVVFIDSFLDSVVQPKGWLEWPGVPENVLYYAEYNNSGGGANTSQRVNWPGY 286

Query: 335 HVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           HVL N+ EV  F+V  FI+G QWLP+TGIPFR+GF
Sbjct: 287 HVLDNAREVATFSVEMFINGTQWLPQTGIPFRAGF 317

BLAST of Lag0001594.1 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 252.3 bits (643), Expect = 6.0e-67
Identity = 125/284 (44.01%), Postives = 185/284 (65.14%), Query Frame = 0

Query: 88  LWSGSNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMA 147
           +++ +  +   V  ++ K  I LIGD  + T+I  N S   G++T  S+T  V G  F+A
Sbjct: 341 IYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVA 400

Query: 148 KFLTFQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDF 207
             +TF+N+AG +K QAVAV + A+ + +Y+C F GYQDTLY  +L QF+++CDIYGTIDF
Sbjct: 401 VDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDF 460

Query: 208 IFGDGLAVFQDCNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSS 267
           IFG+  A+FQ+CN+YAR  +A+ ++ VTA  + + N  +G    NC +  +P++     S
Sbjct: 461 IFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKS 520

Query: 268 SKDNVTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWS-STPLNLLFYAEYNNSGPGAD 327
           +      FLGRPW+ YS  V+++S++  VV P GWL+W+ +T L+ + Y EY+N GPGAD
Sbjct: 521 T----MTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGAD 580

Query: 328 TSRRVTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           TS+RV W GY +L N ++   FTV NF  G+ WLP+T IPF  G
Sbjct: 581 TSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQTDIPFYGG 619

BLAST of Lag0001594.1 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 250.8 bits (639), Expect = 1.8e-66
Identity = 121/280 (43.21%), Postives = 182/280 (65.00%), Query Frame = 0

Query: 92  SNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLT 151
           S  +   V    +K  + +IGD  + T++  NR+   G++T  SAT  V   NF+A  +T
Sbjct: 296 SGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMT 355

Query: 152 FQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGD 211
           F+N+AG +K QAVA+  +A+ + +Y C F  YQDTLY  +L QF+++CDIYGT+DFIFG+
Sbjct: 356 FRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGN 415

Query: 212 GLAVFQDCNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDN 271
              VFQDCNLY R  + +  + +TAQ + + N ++G    NC +  + ++     SS   
Sbjct: 416 AAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV----SSNYT 475

Query: 272 VTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSRR 331
           V  +LGRPW++YS  VFM+S++D VV P GW +W+    L+ L+YAEYNN+G G+ T+ R
Sbjct: 476 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 535

Query: 332 VTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           V WPGYHV+ NS + + FTV NF+ G+ W+ ++G+P+ SG
Sbjct: 536 VVWPGYHVI-NSTDANNFTVENFLLGDGWMVQSGVPYISG 570

BLAST of Lag0001594.1 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 249.6 bits (636), Expect = 3.9e-66
Identity = 121/273 (44.32%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 99  VEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSAGA 158
           VE    K  I ++GD    T+I  NRS   G++T  SAT  V+G+ F+A+ +TFQN+AG 
Sbjct: 256 VEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGP 315

Query: 159 DKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVFQD 218
           +K QAVA+    +   +Y+C   GYQDTLYA ++ QFF++C I GT+DFIFGD  AVFQ 
Sbjct: 316 EKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQS 375

Query: 219 CNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFLGR 278
           C + A+  L + ++++TAQ + + N+ +GF  Q  N+    ++  + +++      +LGR
Sbjct: 376 CQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT----ATYLGR 435

Query: 279 PWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSRRVTWPGYH 338
           PW+ YS  VFM++++   + P GWL+W+    L+ L+Y EY NSGPGA   RRV WPGYH
Sbjct: 436 PWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYH 495

Query: 339 VLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           VL+ S E + FTV   I GN WLP TGI F +G
Sbjct: 496 VLNTSAEANNFTVSQLIQGNLWLPSTGITFIAG 524

BLAST of Lag0001594.1 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 249.6 bits (636), Expect = 3.9e-66
Identity = 127/282 (45.04%), Postives = 174/282 (61.70%), Query Frame = 0

Query: 91  GSNQFRNVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFL 150
           G   F N VE    K +I  IGD    T+I  NRS   G+ST  + T+ V G+ ++AK +
Sbjct: 256 GGEYFEN-VELPKKKTMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDI 315

Query: 151 TFQNSAGADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFG 210
           +F NSAG  K QAVA    ++H+A+Y+C F GYQDTLY  +  QF+++CDIYGTIDFIFG
Sbjct: 316 SFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFG 375

Query: 211 DGLAVFQDCNLYARLADA--RSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKD 270
           +   VFQ+ +LYAR  +   +   TAQS+   +  +G    NC +  +P++       K+
Sbjct: 376 NAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLI----PVKE 435

Query: 271 NVTLFLGRPWRDYSTVVFMESFLDGVVPPEGWLDWSST-PLNLLFYAEYNNSGPGADTSR 330
           N   +LGRPWR YS  V ++SF+D ++ P GWL+      L  L+Y EY N GPGA+ ++
Sbjct: 436 NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAK 495

Query: 331 RVTWPGYHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSGF 370
           RVTWPG+  + N  E  +FTVG FIDG+ WL  TGIPF  GF
Sbjct: 496 RVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLGF 532

BLAST of Lag0001594.1 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 249.2 bits (635), Expect = 5.1e-66
Identity = 123/275 (44.73%), Postives = 176/275 (64.00%), Query Frame = 0

Query: 97  NVVEFNSSKDIIALIGDDPSTTLIVDNRSNHTGFSTPTSATLTVNGQNFMAKFLTFQNSA 156
           NVV   S K+++ +IGD   +T++  NR+   G +T  SAT  V+G  F+A+ +TF+N+A
Sbjct: 293 NVVIKKSIKNVM-VIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTA 352

Query: 157 GADKGQAVAVFDTANHTAYYKCVFLGYQDTLYAGALPQFFKKCDIYGTIDFIFGDGLAVF 216
           G +K QAVA+  +++ + +Y C F GYQDTLY  +  QF + C+IYGT+DFIFGD  A+ 
Sbjct: 353 GPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAIL 412

Query: 217 QDCNLYAR--LADARSTVTAQSKANKNDSSGFVFQNCNVTVSPEITTSTSSSKDNVTLFL 276
           Q+CN+YAR  ++  ++T+TAQS+   ++++GFV Q+  V  + E              +L
Sbjct: 413 QNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE-------------TYL 472

Query: 277 GRPWRDYSTVVFMESFLDGVVPPEGWLDWS-STPLNLLFYAEYNNSGPGADTSRRVTWPG 336
           GRPWR +S  VFM+  L  +V P GWL WS S  L+ L+Y EY N+G GA  S RV WPG
Sbjct: 473 GRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRVKWPG 532

Query: 337 YHVLHNSMEVDKFTVGNFIDGNQWLPETGIPFRSG 369
           YHV+    E +KFTV NF+DGN W+  TG+P   G
Sbjct: 533 YHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDG 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150332.15.2e-11372.63probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia][more]
XP_011654361.26.4e-11170.55probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus][more]
XP_022150325.13.2e-11072.22probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia][more]
XP_023546999.14.1e-11070.18pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_031741746.19.2e-11070.55probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE864851... [more]
Match NameE-valueIdentityDescription
O048872.0e-6746.77Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q9FF778.5e-6644.01Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK72.5e-6543.21Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9LXK75.5e-6544.32Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
O814155.5e-6545.04Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1DAG52.5e-11372.63Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1[more]
A0A6J1D8631.5e-11072.22Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1[more]
A0A0A0L1M34.5e-11070.18Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1[more]
A0A6J1KJG61.3e-10969.82Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111493749 PE=4 SV=1[more]
A0A5D3C2R61.7e-10969.45Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 ... [more]
Match NameE-valueIdentityDescription
AT5G04970.16.0e-6744.01Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.11.8e-6643.21Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G43270.13.9e-6644.32Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.13.9e-6645.04Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G23200.15.1e-6644.73Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 95..355
e-value: 1.3E-89
score: 299.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 91..369
e-value: 3.9E-91
score: 307.7
NoneNo IPR availablePANTHERPTHR31707:SF149PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 87..367
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 87..367
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 201..210
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 99..367

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0001594Lag0001594gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0001594.1.exon5Lag0001594.1.exon5exon
Lag0001594.1.exon4Lag0001594.1.exon4exon
Lag0001594.1.exon3Lag0001594.1.exon3exon
Lag0001594.1.exon2Lag0001594.1.exon2exon
Lag0001594.1.exon1Lag0001594.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0001594.1cds.Lag0001594.1_5CDS
cds.Lag0001594.1cds.Lag0001594.1_4CDS
cds.Lag0001594.1cds.Lag0001594.1_3CDS
cds.Lag0001594.1cds.Lag0001594.1_2CDS
cds.Lag0001594.1cds.Lag0001594.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0001594.1Lag0001594.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity