Lag0001481.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0001481.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr4: 31847311 .. 31849900 (+)
Sequence length1611
RNA-Seq ExpressionLag0001481.1
SyntenyLag0001481.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAAGCCGTATCAAGTCAATGCGTCTCAATACCTTCACATGTTCTTTGGACTCCCATCGTTCTTTAATCGTCTCGATGTTGCCAGAATCACGTCGTATTCGACTCCAAATCCCATTCAGAACAAGCTTTTACCGGAAAATCACGAATTCTATTCAAAACTTCTGCAAGTTTGCTTGCAACAATGCAAGAGAATCAAAGCCCACAACCTGTTCGACGAAAAGCCTAAGCCAGCTCTTCAAGCATTGAGAACCCGAAGGTCATCCATTCGAAGAGTTTGAAGTTTGGAGTTGGGCTCAAAGGGTTGCTAGGCAATGCCATTGTTGACCTTTATGTCAAATGCGACAATGTGGACTTCGCTCAGAAGCATTTTCCCAGCTTGAGAAAAAGGATGTATTTGCCTGAACTCGATGCTTTCTATGTACTCGAAGCATGGTTTATTTGCAACTGTTATTGAATCTTTTGTGTCAATGTGGAATCATGGGGTGCGGCCTAACGAGTTCACGTTTGCGATGGTTTTATCAGCTTGTTCTAGGTTGGGGGATGTTATCTTGGTAGACAAGTCCACTGTGGTGTTTTTAAGATGGGTTTTGGGTTTCGTTCTTTTTGTCAAGGTGGGCTAATTGATATGTATGCCAAATGCCGTAGTCTAAGAGATGCTCGCTTGGTGTTTGATGGGGCACTTAATTTAGATACTGTTTCATGGACGCCTTGATTGCAGGGTATGTTCAAGATGGCTTACCTGAGGAGGAATCAAGGTGTTCGATAGAATGCAGAGAGTTGGCCATGTACTTGATCAGGTAGCACTTGTGACTGTTATAAATGCTTATGTGGCTCTAGATATGCTTGATGATGCTTGTGAGTTGTTTGCTCAGATGCCAACCCTAATATTGTAGCTTGGAATGTGATGATTTCAGGGCATGCTAAGAGGGGATTTTCAGAGGAAGCTATCTCATTTTTTCTGAAATTAAAGAGAGTTGGCTTAAAAGCCACTAGATCTACTATAGGAAGTGTTTTAAGTGCAATTGCCAGTTTATCGATGCTGAATTACGGCTTAATGGTTCATGCTCAGGTGATTAAGGAAGGGCTGAATGCTAATGTGTATGTAGGAAGTGCATTGGTGAACATGTATGCCAAATGTGAAAAAATGGATGCTGCAAAAGAAGTGTTCAATTCTTTAGATGAGAGAAATATTGTCTTGTGGAATGCTATGCTTGGAGGTTTTTCACAGAACGGGCTTGTCAGTGAAGTGATGGAATTTTTCTCATGTATGAAACAGTTTGGACCACAACCTGATGAGTTTACCTACACTAGTATTTTCAGTGCATGTGCCTCCTTGCAGTATCTTGATTTCGGTCGTCAACTTCACACTGTTATGATAAAGAACAAATTTGCATCTAATATATTTGTTTCAAATGCATTGGTAGACATGTATGCTAAATCAGGGGCTTTAAAAGAAGCAAGAAAACAATTTGAGTTGATGAAAGTTCATGACAATGTTTCATGGAATGCAATAATTGTGGGGTATGTGCAGGAAGAGTATAATGATGAGGCGTTTTTCATGTTTCGAAGAATGGTTTCAAATGGGGCTGTTCCAGATGAGGTGTCTTTGGCCAGCATAGTGAGTGCTTGTGCAAATGTTCAGAGTTTAAACGAGGCAACAATGTCACTGTCTCTTAGTTAAAGTTGGTTTGGATACAAGCATTTGTTCTGGAAGTTCCCTAATTGATATGTATGTCAAGTGTGGGGTTTTTTCGGCAGCCCATGATGTCTTTTATTCTATGCCCTCTAGAAGTGTTGTCTCAATAAACGCTTGATTGCTGGCTACACTATGAACCACTTAGAGGAAGCCATATATCTATTTCAAGAGATGCAGATGGTTGGACTTAAACCCACAGAAGTTACATTTGCAGGGCTTTTAGATGGATGTGATCGAGCATCTATGCTAAAACTAGGAAGGCAAATTCACTGTCAAGTTATGAAGTGGGGTTTTCTATTTGGTAGTGAAATGGTGTGTGTGTCTCTCTTGTGCATGTATATGAGCTCCCAAAGATTCTCAGACTCAGAAACTCTCTTCTCTGAGTTGCAGTATCCAAAAAGTTTAGTGTTATGGACTGCTTTCATTTCAGGATATTCTCAAAACAATCACTATGAGCAGGCTTTGCGATTCTATCAACATATGCGATCAGAGAATATTTACCGGACCAAGCAACATTTGCCAGTGTTCTTCGAGCATGTGCTGGATTGTCTTCTTTAGAAAACGGTCAAGAGATTCATTCCCTTATCCTCCATACTGGCTTTAACATGGATGAAATAACCTGTAGTGCTCTTATAGACATGTATGCAAAATGTGGCGATGTTAAAAGCTCCATTAAGGTTTTTCATGAAATGGGAAGTAAAAACAGTGTCGTTTCTTGGAACTCCATGATAGTTGGACTTGCTAAGAATGGCTATGCAGAAGAAGCACTAGAAATATTTACTCAAATGGAGAAAGAATCCATCATGCCTGATGATGTCACATTCCTTGGTGTTCTTTCTGCTTGTAGCCATGCTGGGCGAGTGTCGAAGGCCGAAAGATCTTCGACCTGA

mRNA sequence

ATGTGGAAGCCGTATCAAGTCAATGCGTCTCAATACCTTCACATGTTCTTTGGACTCCCATCGTTCTTTAATCGTCTCGATGTTGCCAGAATCACGTCGTATTCGACTCCAAATCCCATTCAGAACAAGCTTTTACCGGAAAATCACGAATTCTATTCAAAACTTCTGCAAGTTTGCTTGCAACAATGCAAGAGAATCAAAGCCCACAACCTGTTCGACGAAAAGCCTAAGCCAGCTCTTCAAGCATTGAGAACCCGAAGGTTGGGGGATGTTATCTTGGTAGACAAGTCCACTGTGGTGTTTTTAAGATGGGTTTTGGGTTTCGTTCTTTTTGTCAAGGGTATGTTCAAGATGGCTTACCTGAGGAGGAATCAAGATGCCAACCCTAATATTGTAGCTTGGAATGTGATGATTTCAGGGCATGCTAAGAGGGGATTTTCAGAGGAAGCTATCTCATTTTTTCTGAAATTAAAGAGAGTTGGCTTAAAAGCCACTAGATCTACTATAGGAAGTGTTTTAAGTGCAATTGCCAGTTTATCGATGCTGAATTACGGCTTAATGGTTCATGCTCAGGTGATTAAGGAAGGGCTGAATGCTAATGTGTATGTAGGAAGTGCATTGGTGAACATGTATGCCAAATGTGAAAAAATGGATGCTGCAAAAGAAGTGTTCAATTCTTTAGATGAGAGAAATATTGTCTTGTGGAATGCTATGCTTGGAGGTTTTTCACAGAACGGGCTTGTCAGTGAAGTGATGGAATTTTTCTCATGTATGAAACAGTTTGGACCACAACCTGATGAGTTTACCTACACTAGTATTTTCAGTGCATGTGCCTCCTTGCAGTATCTTGATTTCGGTCGTCAACTTCACACTGTTATGATAAAGAACAAATTTGCATCTAATATATTTGTTTCAAATGCATTGGTAGACATGTATGCTAAATCAGGGGCTTTAAAAGAAGCAAGAAAACAATTTGAGTTGATGAAAGTTCATGACAATGTTTCATGGAATGCAATAATTGTGGGGTATGTGCAGGAAGAGTATAATGATGAGGCGTTTTTCATGTTTCGAAGAATGGTTTCAAATGGGGCTGTTCCAGATGAGGTGTCTTTGGCCAGCATAGTGAGTGCTTGTGCAAATGTTCAGAGTTTAAACGAGGCAACAATGTCACTGTCTCTTAGCTTTGCGATTCTATCAACATATGCGATCAGAGAATATTTACCGGACCAAGCAACATTTGCCAGTGTTCTTCGAGCATGTGCTGGATTGTCTTCTTTAGAAAACGGTCAAGAGATTCATTCCCTTATCCTCCATACTGGCTTTAACATGGATGAAATAACCTGTAGTGCTCTTATAGACATGTATGCAAAATGTGGCGATGTTAAAAGCTCCATTAAGGTTTTTCATGAAATGGGAAGTAAAAACAGTGTCGTTTCTTGGAACTCCATGATAGTTGGACTTGCTAAGAATGGCTATGCAGAAGAAGCACTAGAAATATTTACTCAAATGGAGAAAGAATCCATCATGCCTGATGATGTCACATTCCTTGGTGTTCTTTCTGCTTGTAGCCATGCTGGGCGAGTGTCGAAGGCCGAAAGATCTTCGACCTGA

Coding sequence (CDS)

ATGTGGAAGCCGTATCAAGTCAATGCGTCTCAATACCTTCACATGTTCTTTGGACTCCCATCGTTCTTTAATCGTCTCGATGTTGCCAGAATCACGTCGTATTCGACTCCAAATCCCATTCAGAACAAGCTTTTACCGGAAAATCACGAATTCTATTCAAAACTTCTGCAAGTTTGCTTGCAACAATGCAAGAGAATCAAAGCCCACAACCTGTTCGACGAAAAGCCTAAGCCAGCTCTTCAAGCATTGAGAACCCGAAGGTTGGGGGATGTTATCTTGGTAGACAAGTCCACTGTGGTGTTTTTAAGATGGGTTTTGGGTTTCGTTCTTTTTGTCAAGGGTATGTTCAAGATGGCTTACCTGAGGAGGAATCAAGATGCCAACCCTAATATTGTAGCTTGGAATGTGATGATTTCAGGGCATGCTAAGAGGGGATTTTCAGAGGAAGCTATCTCATTTTTTCTGAAATTAAAGAGAGTTGGCTTAAAAGCCACTAGATCTACTATAGGAAGTGTTTTAAGTGCAATTGCCAGTTTATCGATGCTGAATTACGGCTTAATGGTTCATGCTCAGGTGATTAAGGAAGGGCTGAATGCTAATGTGTATGTAGGAAGTGCATTGGTGAACATGTATGCCAAATGTGAAAAAATGGATGCTGCAAAAGAAGTGTTCAATTCTTTAGATGAGAGAAATATTGTCTTGTGGAATGCTATGCTTGGAGGTTTTTCACAGAACGGGCTTGTCAGTGAAGTGATGGAATTTTTCTCATGTATGAAACAGTTTGGACCACAACCTGATGAGTTTACCTACACTAGTATTTTCAGTGCATGTGCCTCCTTGCAGTATCTTGATTTCGGTCGTCAACTTCACACTGTTATGATAAAGAACAAATTTGCATCTAATATATTTGTTTCAAATGCATTGGTAGACATGTATGCTAAATCAGGGGCTTTAAAAGAAGCAAGAAAACAATTTGAGTTGATGAAAGTTCATGACAATGTTTCATGGAATGCAATAATTGTGGGGTATGTGCAGGAAGAGTATAATGATGAGGCGTTTTTCATGTTTCGAAGAATGGTTTCAAATGGGGCTGTTCCAGATGAGGTGTCTTTGGCCAGCATAGTGAGTGCTTGTGCAAATGTTCAGAGTTTAAACGAGGCAACAATGTCACTGTCTCTTAGCTTTGCGATTCTATCAACATATGCGATCAGAGAATATTTACCGGACCAAGCAACATTTGCCAGTGTTCTTCGAGCATGTGCTGGATTGTCTTCTTTAGAAAACGGTCAAGAGATTCATTCCCTTATCCTCCATACTGGCTTTAACATGGATGAAATAACCTGTAGTGCTCTTATAGACATGTATGCAAAATGTGGCGATGTTAAAAGCTCCATTAAGGTTTTTCATGAAATGGGAAGTAAAAACAGTGTCGTTTCTTGGAACTCCATGATAGTTGGACTTGCTAAGAATGGCTATGCAGAAGAAGCACTAGAAATATTTACTCAAATGGAGAAAGAATCCATCATGCCTGATGATGTCACATTCCTTGGTGTTCTTTCTGCTTGTAGCCATGCTGGGCGAGTGTCGAAGGCCGAAAGATCTTCGACCTGA

Protein sequence

MWKPYQVNASQYLHMFFGLPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKPALQALRTRRLGDVILVDKSTVVFLRWVLGFVLFVKGMFKMAYLRRNQDANPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENGQEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVGLAKNGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSKAERSST
Homology
BLAST of Lag0001481.1 vs. NCBI nr
Match: XP_038899907.1 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Benincasa hispida])

HSP 1 Score: 648.7 bits (1672), Expect = 4.5e-182
Identity = 410/815 (50.31%), Postives = 456/815 (55.95%), Query Frame = 0

Query: 18  GLPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPK 77
           GLPSFFN L+VARITSYS P+ + N+ L +N   +S+LLQ+CLQ C RI+AHNLFDEKPK
Sbjct: 11  GLPSFFNPLNVARITSYSIPDRVCNQQLTKNRNPHSELLQICLQHCGRIQAHNLFDEKPK 70

Query: 78  PALQALRTRR----------------LGDVIL------------------VDKSTVVFLR 137
             LQALR  +                LG+ I+                  ++K  V    
Sbjct: 71  SLLQALRIAKVIHSKSLEIGVGLKGLLGNAIVDLYVKCGDVDFAQKAFSRLEKKDVFACN 130

Query: 138 WVLG-----------FVLFVK----------------------------------GMFKM 197
            VL            F  FV                                   G+FKM
Sbjct: 131 SVLSMYSKQGLFATVFQSFVSMWNHGVRPNEFTFAMVLSACSRLQDVNYGRQVHCGVFKM 190

Query: 198 AY--------------------------------------------------LRRNQDA- 257
            +                                                  L R  DA 
Sbjct: 191 GFGFRSFCQALIAGYVRDGFPKEAVKVFDRMQRVGHVPDQIALVTVINAYVALDRLADAR 250

Query: 258 -------NPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLS 317
                  NPNIVAWNVMISGHAKRG +EEAISFFL+LKR GLKATRST+GSVLSAIASLS
Sbjct: 251 KLFTQMPNPNIVAWNVMISGHAKRGCAEEAISFFLELKRAGLKATRSTLGSVLSAIASLS 310

Query: 318 MLNYGLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLG 377
           M NYG MVHAQVIKEGLN NVYVGSALVNMYAKCEKMDAAK+VF+SL ERN++LWNAMLG
Sbjct: 311 MHNYGSMVHAQVIKEGLNDNVYVGSALVNMYAKCEKMDAAKQVFDSLSERNLILWNAMLG 370

Query: 378 GFSQNGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFAS 437
           GF+QNGL  EVMEFFS MK+ GPQPDEFT TSIFSACAS+QYLDFGRQLHT MIKNKFAS
Sbjct: 371 GFAQNGLAHEVMEFFSYMKRHGPQPDEFTLTSIFSACASMQYLDFGRQLHTFMIKNKFAS 430

Query: 438 NIFVSNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMV 497
           N+FV+NALVDMYAKSGALKEARKQFELMK+HDNVSWNAIIVGYVQEEYNDEAFFMFRRM+
Sbjct: 431 NLFVANALVDMYAKSGALKEARKQFELMKIHDNVSWNAIIVGYVQEEYNDEAFFMFRRMI 490

Query: 498 SNGAVPDEVSLASIVSACANVQS------------------------------------- 534
           SNGA+PDEVSLASIVSACA +Q                                      
Sbjct: 491 SNGALPDEVSLASIVSACAKLQEFKGGQQCHGLLVKVGLDTRICAGSSLIDMYVKCGVLL 550

BLAST of Lag0001481.1 vs. NCBI nr
Match: XP_022974928.1 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita maxima])

HSP 1 Score: 641.7 bits (1654), Expect = 5.5e-180
Identity = 408/845 (48.28%), Postives = 453/845 (53.61%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N  +VARI SYSTP+ I N+LL E+H  +SK LQVCLQ C+RIK HNLFDEKPKP
Sbjct: 15  LPSFLNPHNVARIASYSTPDCISNQLLSEDHNTHSKFLQVCLQHCRRIKPHNLFDEKPKP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR--------WVL 138
            LQALRT +                LG+ I+        VD +  VF R        W  
Sbjct: 75  VLQALRTAKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKVFSRLEKKDVFAWNS 134

Query: 139 GFVLFVK-----------------------------------------------GMFKMA 198
              ++ K                                               G+FKM 
Sbjct: 135 VLSMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCGVFKMG 194

Query: 199 Y----------------------------------------------------------- 258
           +                                                           
Sbjct: 195 FGFSSFCQGGLIDMYAKCHDLRDARLVFDGALNMDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+ E
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFALE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGL+ NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLHDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAATEVFNSLEERNIVLWNAMLAGFAQNGLAHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALK+ARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFVSNLFVANALVDMYAKSGALKDARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYN EAFFMFRRMV NGA+PDEVSLA+IVSACANVQ L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNGEAFFMFRRMVCNGALPDEVSLATIVSACANVQELKPGQQ 554

BLAST of Lag0001481.1 vs. NCBI nr
Match: XP_023534935.1 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.4 bits (1648), Expect = 2.7e-179
Identity = 408/845 (48.28%), Postives = 454/845 (53.73%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N   VARI SYSTP+ I N++L E+H  +S+LLQ+CL  C+RIKAHNLFDEKP+P
Sbjct: 15  LPSFLNPPHVARIASYSTPDYISNQVLTEDHNPHSELLQLCLHHCRRIKAHNLFDEKPEP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVF------------- 138
            L+ALRT +                LG+ I+        VD +  +F             
Sbjct: 75  VLRALRTSKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKLFSRLEKKDVFSWNS 134

Query: 139 --------------------------------------------------------LRWV 198
                                                                   L+  
Sbjct: 135 VLSMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCSVLKMG 194

Query: 199 LGFVLFVKG----MFKMAY----------------------------------------- 258
           LGF  F +G    M+   +                                         
Sbjct: 195 LGFSSFCQGGLIDMYAKCHDLRDARLVFDGALNMDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+ E
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFALE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLN NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAANEVFNSLEERNIVLWNAMLAGFAQNGLPHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALK+ARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFISNLFVANALVDMYAKSGALKDARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYNDEAF MFRRMVSNGA+PDEVSLASIVSACANV  L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNDEAFIMFRRMVSNGALPDEVSLASIVSACANVHELKPGQQ 554

BLAST of Lag0001481.1 vs. NCBI nr
Match: KAG6592177.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.0 bits (1634), Expect = 1.1e-177
Identity = 408/845 (48.28%), Postives = 451/845 (53.37%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N  +V RI S ST + I N+ L E+H  +S+LLQ+CL  C+RIKAHNLFDEKP+P
Sbjct: 15  LPSFLNPPNVDRIASCSTSDCISNQFLYEDHNPHSELLQLCLHHCRRIKAHNLFDEKPEP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR--------WVL 138
            LQ LRT +                LG+ I+        VD +  VF R        W  
Sbjct: 75  VLQVLRTAKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKVFSRLEKKDVFAWNS 134

Query: 139 GFVLFVK-----------------------------------------------GMFKMA 198
              ++ K                                               G+FKM 
Sbjct: 135 VISMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCGVFKMG 194

Query: 199 Y----------------------------------------------------------- 258
           +                                                           
Sbjct: 195 FGFSSFCQGGLIDMYAKCHDLRDARLVFDGAIYLDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+EE
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFAEE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVH+QVIKEGLN NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHSQVIKEGLNDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAANEVFNSLEERNIVLWNAMLAGFAQNGLAHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALKEARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFVSNLFVANALVDMYAKSGALKEARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYNDEAF MFRRMV NG +PDEVSLAS VSACANVQ L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNDEAFSMFRRMVCNGDLPDEVSLASTVSACANVQELKPGQQ 554

BLAST of Lag0001481.1 vs. NCBI nr
Match: XP_022935750.1 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita moschata])

HSP 1 Score: 634.0 bits (1634), Expect = 1.1e-177
Identity = 408/845 (48.28%), Postives = 451/845 (53.37%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N  +VARI S ST + I N+ L E+H  +S+LLQ+CL  C+RIKAHNLFDEKP+P
Sbjct: 15  LPSFLNPPNVARIASCSTSDCISNQFLYEDHNPHSELLQLCLHHCRRIKAHNLFDEKPEP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR--------WVL 138
            LQ LRT +                LG+ I+        VD +  VF R        W  
Sbjct: 75  VLQVLRTAKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKVFSRLEKKDVFAWNS 134

Query: 139 GFVLFVK-----------------------------------------------GMFKMA 198
              ++ K                                               G+FKM 
Sbjct: 135 VLSMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCGVFKMG 194

Query: 199 Y----------------------------------------------------------- 258
           +                                                           
Sbjct: 195 FGFSSFCQGGLIDMYAKCHDLRDARLVFDGAIYLDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+EE
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFAEE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVH+QVIKEGLN NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHSQVIKEGLNDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAANEVFNSLEERNIVLWNAMLAGFAQNGLAHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALKEARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFVSNLFVANALVDMYAKSGALKEARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYNDEAF MFRRMV NG +PDEVSLAS VSACANVQ L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNDEAFSMFRRMVCNGDLPDEVSLASTVSACANVQELKPGQQ 554

BLAST of Lag0001481.1 vs. ExPASy Swiss-Prot
Match: Q9SS83 (Pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E88 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.9e-103
Identity = 230/581 (39.59%), Postives = 307/581 (52.84%), Query Frame = 0

Query: 114 GMFKMAYLRRNQDANPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVL 173
           G  K A L   + ++P++VAWNVMISGH KRG    AI +F  +++  +K+TRST+GSVL
Sbjct: 275 GKLKDARLLFGEMSSPDVVAWNVMISGHGKRGCETVAIEYFFNMRKSSVKSTRSTLGSVL 334

Query: 174 SAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIV 233
           SAI  ++ L+ GL+VHA+ IK GL +N+YVGS+LV+MY+KCEKM+AA +VF +L+E+N V
Sbjct: 335 SAIGIVANLDLGLVVHAEAIKLGLASNIYVGSSLVSMYSKCEKMEAAAKVFEALEEKNDV 394

Query: 234 LWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVM 293
            WNAM+ G++ NG   +VME F  MK  G   D+FT+TS+ S CA+   L+ G Q H+++
Sbjct: 395 FWNAMIRGYAHNGESHKVMELFMDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQFHSII 454

Query: 294 IKNKFASNIFVSNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAF 353
           IK K A N+FV NALVDMYAK GAL++AR+ FE M   DNV+WN II  YVQ+E   EAF
Sbjct: 455 IKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAF 514

Query: 354 FMFRRM-----VSNGAV------------------------------------------- 413
            +F+RM     VS+GA                                            
Sbjct: 515 DLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMY 574

Query: 414 ----------------------------------------------------PDEVSLAS 473
                                                               P E++ A+
Sbjct: 575 SKCGIIKDARKVFSSLPEWSVVSMNALIAGYSQNNLEEAVVLFQEMLTRGVNPSEITFAT 634

Query: 474 IVSACANVQSLNEATM-------------SLSLSFAILSTY------------------- 533
           IV AC   +SL   T                 L  ++L  Y                   
Sbjct: 635 IVEACHKPESLTLGTQFHGQITKRGFSSEGEYLGISLLGMYMNSRGMTEACALFSELSSP 694


HSP 2 Score: 128.6 bits (322), Expect = 2.1e-28
Identity = 119/494 (24.09%), Postives = 220/494 (44.53%), Query Frame = 0

Query: 21  SFFNRLDVARITSYSTP--NPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 80
           + F+     R  SYS      I   +LP + + + +LL++CL QCK  K+  +FDE P+ 
Sbjct: 12  AMFDSFSFVRRLSYSPDLGRRIYGHVLPSHDQIHQRLLEICLGQCKLFKSRKVFDEMPQR 71

Query: 81  ALQALRTRRLGDVILVDKSTVVFL----RWVLGFVLFVKGMFKMAYLRRNQD-ANPNIVA 140
              ALR  +     +  KS ++ +    R     V       +++Y  +  D    ++ A
Sbjct: 72  LALALRIGK----AVHSKSLILGIDSEGRLGNAIVDLYAKCAQVSYAEKQFDFLEKDVTA 131

Query: 141 WNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVI 200
           WN M+S ++  G   + +  F+ L    +   + T   VLS  A  + + +G  +H  +I
Sbjct: 132 WNSMLSMYSSIGKPGKVLRSFVSLFENQIFPNKFTFSIVLSTCARETNVEFGRQIHCSMI 191

Query: 201 KEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVME 260
           K GL  N Y G ALV+MYAKC+++  A+ VF  + + N V W  +  G+ + GL  E + 
Sbjct: 192 KMGLERNSYCGGALVDMYAKCDRISDARRVFEWIVDPNTVCWTCLFSGYVKAGLPEEAVL 251

Query: 261 FFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYA 320
            F  M+  G +PD   + ++ +    L  L   R L   M     + ++   N ++  + 
Sbjct: 252 VFERMRDEGHRPDHLAFVTVINTYIRLGKLKDARLLFGEM----SSPDVVAWNVMISGHG 311

Query: 321 KSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLAS 380
           K G    A + F  M+   +V      +G V       A      +V   A+  ++ LAS
Sbjct: 312 KRGCETVAIEYFFNMR-KSSVKSTRSTLGSVLSAIGIVANLDLGLVVHAEAI--KLGLAS 371

Query: 381 IVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENGQEIH 440
            +   +++ S+      +  +  +    A+ E   +   + +++R  A         E+ 
Sbjct: 372 NIYVGSSLVSMYSKCEKMEAAAKVFE--ALEE--KNDVFWNAMIRGYAHNGESHKVMELF 431

Query: 441 SLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVS----WNSMIVGLAK 500
             +  +G+N+D+ T ++L+   A   D++   + FH +  K  +       N+++   AK
Sbjct: 432 MDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQ-FHSIIIKKKLAKNLFVGNALVDMYAK 489

Query: 501 NGYAEEALEIFTQM 504
            G  E+A +IF +M
Sbjct: 492 CGALEDARQIFERM 489

BLAST of Lag0001481.1 vs. ExPASy Swiss-Prot
Match: P93005 (Pentatricopeptide repeat-containing protein At2g33680 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E19 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 4.0e-72
Identity = 152/404 (37.62%), Postives = 232/404 (57.43%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAI---SFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGL 189
           N   W+ M+SG+A RG  EEAI   + FL+ K  G   +     +VLS++A+   +  G 
Sbjct: 183 NTYTWSTMVSGYATRGRVEEAIKVFNLFLREKEEG-SDSDYVFTAVLSSLAATIYVGLGR 242

Query: 190 MVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNG 249
            +H   IK GL   V + +ALV MY+KCE ++ A ++F+S  +RN + W+AM+ G+SQNG
Sbjct: 243 QIHCITIKNGLLGFVALSNALVTMYSKCESLNEACKMFDSSGDRNSITWSAMVTGYSQNG 302

Query: 250 LVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSN 309
              E ++ FS M   G +P E+T   + +AC+ + YL+ G+QLH+ ++K  F  ++F + 
Sbjct: 303 ESLEAVKLFSRMFSAGIKPSEYTIVGVLNACSDICYLEEGKQLHSFLLKLGFERHLFATT 362

Query: 310 ALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVP 369
           ALVDMYAK+G L +ARK F+ ++  D   W ++I GYVQ   N+EA  ++RRM + G +P
Sbjct: 363 ALVDMYAKAGCLADARKGFDCLQERDVALWTSLISGYVQNSDNEEALILYRRMKTAGIIP 422

Query: 370 DEVSLASIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSL 429
           ++                                           T ASVL+AC+ L++L
Sbjct: 423 ND------------------------------------------PTMASVLKACSSLATL 482

Query: 430 ENGQEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVG 489
           E G+++H   +  GF ++    SAL  MY+KCG ++    VF    +K+ VVSWN+MI G
Sbjct: 483 ELGKQVHGHTIKHGFGLEVPIGSALSTMYSKCGSLEDGNLVFRRTPNKD-VVSWNAMISG 542

Query: 490 LAKNGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSK 531
           L+ NG  +EALE+F +M  E + PDDVTF+ ++SACSH G V +
Sbjct: 543 LSHNGQGDEALELFEEMLAEGMEPDDVTFVNIISACSHKGFVER 542

BLAST of Lag0001481.1 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.2e-70
Identity = 152/404 (37.62%), Postives = 229/404 (56.68%), Query Frame = 0

Query: 134 WNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVI 193
           WN M+SG A+    EEA+ +F  + + G      +  SVLSA + L+ +N G+ VH+ + 
Sbjct: 120 WNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIA 179

Query: 194 KEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVME 253
           K    ++VY+GSALV+MY+KC  ++ A+ VF+ + +RN+V WN+++  F QNG   E ++
Sbjct: 180 KSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALD 239

Query: 254 FFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKN-KFASNIFVSNALVDMY 313
            F  M +   +PDE T  S+ SACASL  +  G+++H  ++KN K  ++I +SNA VDMY
Sbjct: 240 VFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMY 299

Query: 314 AKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLA 373
           AK   +KEAR  F+ M + + ++  ++I GY        A  MF +M     V     +A
Sbjct: 300 AKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFTKMAERNVVSWNALIA 359

Query: 374 SIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENGQEI 433
                       NE  +SL   F +L   ++    P   +FA++L+ACA L+ L  G + 
Sbjct: 360 GYTQ-----NGENEEALSL---FCLLKRESV---CPTHYSFANILKACADLAELHLGMQA 419

Query: 434 HSLILHTGFNM------DEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVG 493
           H  +L  GF        D    ++LIDMY KCG V+    VF +M  +   VSWN+MI+G
Sbjct: 420 HVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKM-MERDCVSWNAMIIG 479

Query: 494 LAKNGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSK 531
            A+NGY  EALE+F +M +    PD +T +GVLSAC HAG V +
Sbjct: 480 FAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEE 511

BLAST of Lag0001481.1 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-65
Identity = 131/404 (32.43%), Postives = 227/404 (56.19%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVH 189
           ++++WN +I+G+   G +E+ +S F+++   G++   +TI SV +  A   +++ G  VH
Sbjct: 260 DVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVH 319

Query: 190 AQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVS 249
           +  +K   +      + L++MY+KC  +D+AK VF  + +R++V + +M+ G+++ GL  
Sbjct: 320 SIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAG 379

Query: 250 EVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALV 309
           E ++ F  M++ G  PD +T T++ + CA  + LD G+++H  + +N    +IFVSNAL+
Sbjct: 380 EAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALM 439

Query: 310 DMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEV 369
           DMYAK G+++EA   F  M+V D +SWN II GY +  Y +EA  +F  ++         
Sbjct: 440 DMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEE------- 499

Query: 370 SLASIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENG 429
                                             + + PD+ T A VL ACA LS+ + G
Sbjct: 500 ----------------------------------KRFSPDERTVACVLPACASLSAFDKG 559

Query: 430 QEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVGLAK 489
           +EIH  I+  G+  D    ++L+DMYAKCG +  +  +F ++ SK+ +VSW  MI G   
Sbjct: 560 REIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKD-LVSWTVMIAGYGM 619

Query: 490 NGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSKAER 534
           +G+ +EA+ +F QM +  I  D+++F+ +L ACSH+G V +  R
Sbjct: 620 HGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWR 621

BLAST of Lag0001481.1 vs. ExPASy Swiss-Prot
Match: Q9LFI1 (Pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E86 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 4.0e-64
Identity = 147/462 (31.82%), Postives = 241/462 (52.16%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVH 189
           N+V++  +I+G+++ G   EAI  +LK+ +  L   +   GS++ A AS S +  G  +H
Sbjct: 132 NLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQLH 191

Query: 190 AQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVS 249
           AQVIK   ++++   +AL+ MY +  +M  A  VF  +  ++++ W++++ GFSQ G   
Sbjct: 192 AQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFEF 251

Query: 250 EVMEFFSCMKQFGP-QPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNAL 309
           E +     M  FG   P+E+ + S   AC+SL   D+G Q+H + IK++ A N     +L
Sbjct: 252 EALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCSL 311

Query: 310 VDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDE 369
            DMYA+ G L  AR+ F+ ++  D  SWN II G     Y DEA  +F +M S+G +PD 
Sbjct: 312 CDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPDA 371

Query: 370 VSLASIVSACANVQSLNEATM------------SLSLSFAILSTYA-------------- 429
           +SL S++ A     +L++                L++  ++L+ Y               
Sbjct: 372 ISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFED 431

Query: 430 ----------------------------------IREYLPDQATFASVLRACAGLSSLEN 489
                                             + E  PD  T  ++LR C  +SSL+ 
Sbjct: 432 FRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLVSECEPDHITMGNLLRGCVEISSLKL 491

Query: 490 GQEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVGLA 531
           G ++H   L TG   ++   + LIDMYAKCG +  + ++F  M +++ VVSW+++IVG A
Sbjct: 492 GSQVHCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQARRIFDSMDNRD-VVSWSTLIVGYA 551

BLAST of Lag0001481.1 vs. ExPASy TrEMBL
Match: A0A6J1IHS2 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111473755 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 2.7e-180
Identity = 408/845 (48.28%), Postives = 453/845 (53.61%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N  +VARI SYSTP+ I N+LL E+H  +SK LQVCLQ C+RIK HNLFDEKPKP
Sbjct: 15  LPSFLNPHNVARIASYSTPDCISNQLLSEDHNTHSKFLQVCLQHCRRIKPHNLFDEKPKP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR--------WVL 138
            LQALRT +                LG+ I+        VD +  VF R        W  
Sbjct: 75  VLQALRTAKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKVFSRLEKKDVFAWNS 134

Query: 139 GFVLFVK-----------------------------------------------GMFKMA 198
              ++ K                                               G+FKM 
Sbjct: 135 VLSMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCGVFKMG 194

Query: 199 Y----------------------------------------------------------- 258
           +                                                           
Sbjct: 195 FGFSSFCQGGLIDMYAKCHDLRDARLVFDGALNMDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+ E
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFALE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGL+ NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLHDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAATEVFNSLEERNIVLWNAMLAGFAQNGLAHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALK+ARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFVSNLFVANALVDMYAKSGALKDARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYN EAFFMFRRMV NGA+PDEVSLA+IVSACANVQ L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNGEAFFMFRRMVCNGALPDEVSLATIVSACANVQELKPGQQ 554

BLAST of Lag0001481.1 vs. ExPASy TrEMBL
Match: A0A6J1FBI8 (pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111442573 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 5.6e-178
Identity = 408/845 (48.28%), Postives = 451/845 (53.37%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           LPSF N  +VARI S ST + I N+ L E+H  +S+LLQ+CL  C+RIKAHNLFDEKP+P
Sbjct: 15  LPSFLNPPNVARIASCSTSDCISNQFLYEDHNPHSELLQLCLHHCRRIKAHNLFDEKPEP 74

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR--------WVL 138
            LQ LRT +                LG+ I+        VD +  VF R        W  
Sbjct: 75  VLQVLRTAKVIHSKSLKIGVGLNGLLGNAIVDLYVKCGNVDYAQKVFSRLEKKDVFAWNS 134

Query: 139 GFVLFVK-----------------------------------------------GMFKMA 198
              ++ K                                               G+FKM 
Sbjct: 135 VLSMYSKHGLFATVVESFVSMWNDGVRPNEFTFAMVLSACSRLLDVNYGRQVHCGVFKMG 194

Query: 199 Y----------------------------------------------------------- 258
           +                                                           
Sbjct: 195 FGFSSFCQGGLIDMYAKCHDLRDARLVFDGAIYLDTVSWTALIAGYVQDGLPEEAVKVFD 254

Query: 259 -----------------------LRRNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                  L R  DA        NPNIVAWNVMISGHAKRGF+EE
Sbjct: 255 RMQTVGLVPDQIALVTVINAYVALDRLGDARKLFAQLPNPNIVAWNVMISGHAKRGFAEE 314

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LKR GLKATRSTIGSVLSAIASLSMLNYGLMVH+QVIKEGLN NVYVGSALVN
Sbjct: 315 AISFFLELKRTGLKATRSTIGSVLSAIASLSMLNYGLMVHSQVIKEGLNDNVYVGSALVN 374

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKCEKMDAA EVFNSL+ERNIVLWNAML GF+QNGL  EVM+ FS MK++GPQPDEFT
Sbjct: 375 MYAKCEKMDAANEVFNSLEERNIVLWNAMLAGFAQNGLAHEVMDLFSYMKRYGPQPDEFT 434

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASLQYLDFGRQLH VMIKNKF SN+FV+NALVDMYAKSGALKEARKQFELMK
Sbjct: 435 FTSIFSACASLQYLDFGRQLHNVMIKNKFVSNLFVANALVDMYAKSGALKEARKQFELMK 494

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYNDEAF MFRRMV NG +PDEVSLAS VSACANVQ L     
Sbjct: 495 IHDNVSWNAIIVGYVQEEYNDEAFSMFRRMVCNGDLPDEVSLASTVSACANVQELKPGQQ 554

BLAST of Lag0001481.1 vs. ExPASy TrEMBL
Match: A0A0A0K9P1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G320000 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.4e-173
Identity = 395/845 (46.75%), Postives = 448/845 (53.02%), Query Frame = 0

Query: 19  LPSFFNRLDVARITSYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 78
           L SFFN  +VA I++ S PN + N+ L +N   +S+ LQ+CLQ C RI+AHNLFDEKPKP
Sbjct: 13  LSSFFNPPNVATISTNSIPNCVSNQQLVKNLNPHSEFLQICLQHCWRIQAHNLFDEKPKP 72

Query: 79  ALQALRTRR----------------LGDVIL--------VDKSTVVFLR----------- 138
            LQAL T +                LG+VI+        VD +   F R           
Sbjct: 73  VLQALSTAKVIHSKSLKIGVGLKGLLGNVIVDLYVKCGNVDFAQKAFSRLEKKDVFAWNS 132

Query: 139 ---------------------W-------------------------------------V 198
                                W                                      
Sbjct: 133 VLSMYLDHGLFATVVQSFVCMWNHEVRPNEFTFAMVLSACSGLQDVNFGRQVHCGVFKTG 192

Query: 199 LGFVLFVKG-----MFKMAYLR-------------------------------------- 258
            GF  F +G       K  YLR                                      
Sbjct: 193 FGFRSFCQGGLIDMYAKCRYLRDARLVFDGALNLDTVSWTALIAGYVRDGFPMEAVKVFD 252

Query: 259 -------------------------RNQDA--------NPNIVAWNVMISGHAKRGFSEE 318
                                    R  DA        NPN+VAWNVMISGHAKRGF+EE
Sbjct: 253 RMQRVGHAPDQITLVTVVNAYVALGRLADARKLFTQIPNPNVVAWNVMISGHAKRGFAEE 312

Query: 319 AISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVN 378
           AISFFL+LK+ GLKATRS++GSVLSAIASLSMLNYG MVHAQ  KEGL+ NVYVGSALVN
Sbjct: 313 AISFFLELKKTGLKATRSSLGSVLSAIASLSMLNYGSMVHAQATKEGLDDNVYVGSALVN 372

Query: 379 MYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFT 438
           MYAKC KMDAAK+VFNSL ERNIVLWNAMLGGF+QNGL  EVMEFFSCMK+ GPQPDEFT
Sbjct: 373 MYAKCSKMDAAKQVFNSLGERNIVLWNAMLGGFAQNGLAQEVMEFFSCMKRHGPQPDEFT 432

Query: 439 YTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFELMK 498
           +TSIFSACASL YL+FG QLHTVMIKNKFASN+FV+NALVDMYAKSGALKEARKQFELMK
Sbjct: 433 FTSIFSACASLHYLNFGGQLHTVMIKNKFASNLFVANALVDMYAKSGALKEARKQFELMK 492

Query: 499 VHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLASIVSACANVQSL----- 534
           +HDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNG +PDEVSLASIVSACANVQ L     
Sbjct: 493 IHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGVLPDEVSLASIVSACANVQELKRGQQ 552

BLAST of Lag0001481.1 vs. ExPASy TrEMBL
Match: A0A5A7TIQ6 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold93G00290 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.6e-169
Identity = 338/570 (59.30%), Postives = 374/570 (65.61%), Query Frame = 0

Query: 125 QDANPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNY 184
           Q  NPN+VAWNVMISGHAKRGF+EEAISFFL+LK+ GLKATRS++GSVLSAIASLSMLNY
Sbjct: 268 QIPNPNVVAWNVMISGHAKRGFAEEAISFFLELKKTGLKATRSSLGSVLSAIASLSMLNY 327

Query: 185 GLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQ 244
           G MVHAQ IKEGL+ NVYVGSALVNMYAKC KMDAAK+VFNSL ERNIVLWNAMLGGF+Q
Sbjct: 328 GSMVHAQAIKEGLDDNVYVGSALVNMYAKCSKMDAAKQVFNSLGERNIVLWNAMLGGFAQ 387

Query: 245 NGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFV 304
           NGL  EVMEFFS MK+ GPQPDEFT+TSIFSACASL YLDFG QLHTVMIKNKF SN+FV
Sbjct: 388 NGLAQEVMEFFSYMKRHGPQPDEFTFTSIFSACASLHYLDFGGQLHTVMIKNKFTSNLFV 447

Query: 305 SNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGA 364
           +NALVDMYAKSGALKEARKQFE MK+HDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNG 
Sbjct: 448 ANALVDMYAKSGALKEARKQFEFMKIHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGV 507

Query: 365 VPDEVSLASIVSACANVQSLNEA------------------------------------- 424
           +PDEVSLASIVSACANV+   +                                      
Sbjct: 508 LPDEVSLASIVSACANVKEFKQGQQCHCLLVKVGLDTSTCAGSSLIDMYVKCGVVLAARD 567

Query: 425 --------------------TMS------------------------------------- 484
                               TMS                                     
Sbjct: 568 VFYSMPYRNVVSINALIAGYTMSHLEEAIHLFQEIQMVGLKPTEVTFAGLLDGCDGAFML 627

Query: 485 ---------------------------------------------------LSLSFAILS 534
                                                              L +  A++S
Sbjct: 628 NLGRQIHGQVMKWGFLSSSEMVCVSLLCMYMNSQRFADSETLFSELQYPKGLVVWTALIS 687

BLAST of Lag0001481.1 vs. ExPASy TrEMBL
Match: A0A5A7TIQ6 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold93G00290 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.9e-30
Identity = 98/303 (32.34%), Postives = 155/303 (51.16%), Query Frame = 0

Query: 33  SYSTPNPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKPALQALRTRRLGDVI 92
           S S PN + N+ L +    +S+ LQ+CLQ C+RI+AHNLF+EKPK  LQAL T +    +
Sbjct: 7   SNSIPNCVPNQQLVKILSPHSEFLQICLQHCRRIQAHNLFNEKPKAVLQALSTAK----V 66

Query: 93  LVDKSTV--VFLRWVLGFV---LFVK-GMFKMAYLRRNQDANPNIVAWNVMISGHAKRGF 152
           +  KS    V L+ +LG V   L+VK G    A    ++    ++ AWN ++S +   G 
Sbjct: 67  IHSKSLKIGVGLKGLLGNVIVDLYVKCGNVDFAQKAFSRLEKKDVFAWNSVLSMYLDHGL 126

Query: 153 SEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVIKEGLNANVYVGSA 212
               +  F+ +   G++    T   VLSA + L  +NYG  VH  V K G     +    
Sbjct: 127 FATVVQSFVCMWNHGVRPNEFTFAMVLSACSGLQDINYGKQVHCGVFKMGFGFRSFCQGG 186

Query: 213 LVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPD 272
           L++MYAKC  +  A+ VF+     + V W  ++ G+ ++G   E ++ F  M++ G  PD
Sbjct: 187 LIDMYAKCRNLRDARLVFDGALNLDTVSWTTLIAGYVRDGFPMEAVKVFDKMQRVGHVPD 246

Query: 273 EFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYAKSGALKEARKQFE 330
           +    ++ +A  +L  L   R+L T  I N    N+   N ++  +AK G  +EA   F 
Sbjct: 247 QIALVTVINAYVALGRLADARKLFT-QIPN---PNVVAWNVMISGHAKRGFAEEAISFFL 301


HSP 2 Score: 605.9 bits (1561), Expect = 1.6e-169
Identity = 338/570 (59.30%), Postives = 374/570 (65.61%), Query Frame = 0

Query: 125 QDANPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNY 184
           Q  NPN+VAWNVMISGHAKRGF+EEAISFFL+LK+ GLKATRS++GSVLSAIASLSMLNY
Sbjct: 268 QIPNPNVVAWNVMISGHAKRGFAEEAISFFLELKKTGLKATRSSLGSVLSAIASLSMLNY 327

Query: 185 GLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQ 244
           G MVHAQ IKEGL+ NVYVGSALVNMYAKC KMDAAK+VFNSL ERNIVLWNAMLGGF+Q
Sbjct: 328 GSMVHAQAIKEGLDDNVYVGSALVNMYAKCSKMDAAKQVFNSLGERNIVLWNAMLGGFAQ 387

Query: 245 NGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFV 304
           NGL  EVMEFFS MK+ GPQPDEFT+TSIFSACASL YLDFG QLHTVMIKNKF SN+FV
Sbjct: 388 NGLAQEVMEFFSYMKRHGPQPDEFTFTSIFSACASLHYLDFGGQLHTVMIKNKFTSNLFV 447

Query: 305 SNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGA 364
           +NALVDMYAKSGALKEARKQFE MK+HDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNG 
Sbjct: 448 ANALVDMYAKSGALKEARKQFEFMKIHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGV 507

Query: 365 VPDEVSLASIVSACANVQSLNEA------------------------------------- 424
           +PDEVSLASIVSACANV+   +                                      
Sbjct: 508 LPDEVSLASIVSACANVKEFKQGQQCHCLLVKVGLDTSTCAGSSLIDMYVKCGVVLAARD 567

Query: 425 --------------------TMS------------------------------------- 484
                               TMS                                     
Sbjct: 568 VFYSMPYRNVVSINALIAGYTMSHLEEAIHLFQEIQMVGLKPTEVTFAGLLDGCDGAFML 627

Query: 485 ---------------------------------------------------LSLSFAILS 534
                                                              L +  A++S
Sbjct: 628 NLGRQIHGQVMKWGFLSSSEMVCVSLLCMYMNSQRFADSETLFSELQYPKGLVVWTALIS 687

BLAST of Lag0001481.1 vs. TAIR 10
Match: AT3G09040.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-104
Identity = 230/581 (39.59%), Postives = 307/581 (52.84%), Query Frame = 0

Query: 114 GMFKMAYLRRNQDANPNIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVL 173
           G  K A L   + ++P++VAWNVMISGH KRG    AI +F  +++  +K+TRST+GSVL
Sbjct: 275 GKLKDARLLFGEMSSPDVVAWNVMISGHGKRGCETVAIEYFFNMRKSSVKSTRSTLGSVL 334

Query: 174 SAIASLSMLNYGLMVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIV 233
           SAI  ++ L+ GL+VHA+ IK GL +N+YVGS+LV+MY+KCEKM+AA +VF +L+E+N V
Sbjct: 335 SAIGIVANLDLGLVVHAEAIKLGLASNIYVGSSLVSMYSKCEKMEAAAKVFEALEEKNDV 394

Query: 234 LWNAMLGGFSQNGLVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVM 293
            WNAM+ G++ NG   +VME F  MK  G   D+FT+TS+ S CA+   L+ G Q H+++
Sbjct: 395 FWNAMIRGYAHNGESHKVMELFMDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQFHSII 454

Query: 294 IKNKFASNIFVSNALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAF 353
           IK K A N+FV NALVDMYAK GAL++AR+ FE M   DNV+WN II  YVQ+E   EAF
Sbjct: 455 IKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAF 514

Query: 354 FMFRRM-----VSNGAV------------------------------------------- 413
            +F+RM     VS+GA                                            
Sbjct: 515 DLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMY 574

Query: 414 ----------------------------------------------------PDEVSLAS 473
                                                               P E++ A+
Sbjct: 575 SKCGIIKDARKVFSSLPEWSVVSMNALIAGYSQNNLEEAVVLFQEMLTRGVNPSEITFAT 634

Query: 474 IVSACANVQSLNEATM-------------SLSLSFAILSTY------------------- 533
           IV AC   +SL   T                 L  ++L  Y                   
Sbjct: 635 IVEACHKPESLTLGTQFHGQITKRGFSSEGEYLGISLLGMYMNSRGMTEACALFSELSSP 694


HSP 2 Score: 128.6 bits (322), Expect = 1.5e-29
Identity = 119/494 (24.09%), Postives = 220/494 (44.53%), Query Frame = 0

Query: 21  SFFNRLDVARITSYSTP--NPIQNKLLPENHEFYSKLLQVCLQQCKRIKAHNLFDEKPKP 80
           + F+     R  SYS      I   +LP + + + +LL++CL QCK  K+  +FDE P+ 
Sbjct: 12  AMFDSFSFVRRLSYSPDLGRRIYGHVLPSHDQIHQRLLEICLGQCKLFKSRKVFDEMPQR 71

Query: 81  ALQALRTRRLGDVILVDKSTVVFL----RWVLGFVLFVKGMFKMAYLRRNQD-ANPNIVA 140
              ALR  +     +  KS ++ +    R     V       +++Y  +  D    ++ A
Sbjct: 72  LALALRIGK----AVHSKSLILGIDSEGRLGNAIVDLYAKCAQVSYAEKQFDFLEKDVTA 131

Query: 141 WNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVI 200
           WN M+S ++  G   + +  F+ L    +   + T   VLS  A  + + +G  +H  +I
Sbjct: 132 WNSMLSMYSSIGKPGKVLRSFVSLFENQIFPNKFTFSIVLSTCARETNVEFGRQIHCSMI 191

Query: 201 KEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVME 260
           K GL  N Y G ALV+MYAKC+++  A+ VF  + + N V W  +  G+ + GL  E + 
Sbjct: 192 KMGLERNSYCGGALVDMYAKCDRISDARRVFEWIVDPNTVCWTCLFSGYVKAGLPEEAVL 251

Query: 261 FFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALVDMYA 320
            F  M+  G +PD   + ++ +    L  L   R L   M     + ++   N ++  + 
Sbjct: 252 VFERMRDEGHRPDHLAFVTVINTYIRLGKLKDARLLFGEM----SSPDVVAWNVMISGHG 311

Query: 321 KSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLAS 380
           K G    A + F  M+   +V      +G V       A      +V   A+  ++ LAS
Sbjct: 312 KRGCETVAIEYFFNMR-KSSVKSTRSTLGSVLSAIGIVANLDLGLVVHAEAI--KLGLAS 371

Query: 381 IVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENGQEIH 440
            +   +++ S+      +  +  +    A+ E   +   + +++R  A         E+ 
Sbjct: 372 NIYVGSSLVSMYSKCEKMEAAAKVFE--ALEE--KNDVFWNAMIRGYAHNGESHKVMELF 431

Query: 441 SLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVS----WNSMIVGLAK 500
             +  +G+N+D+ T ++L+   A   D++   + FH +  K  +       N+++   AK
Sbjct: 432 MDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQ-FHSIIIKKKLAKNLFVGNALVDMYAK 489

Query: 501 NGYAEEALEIFTQM 504
            G  E+A +IF +M
Sbjct: 492 CGALEDARQIFERM 489

BLAST of Lag0001481.1 vs. TAIR 10
Match: AT2G33680.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.8e-73
Identity = 152/404 (37.62%), Postives = 232/404 (57.43%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAI---SFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGL 189
           N   W+ M+SG+A RG  EEAI   + FL+ K  G   +     +VLS++A+   +  G 
Sbjct: 183 NTYTWSTMVSGYATRGRVEEAIKVFNLFLREKEEG-SDSDYVFTAVLSSLAATIYVGLGR 242

Query: 190 MVHAQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNG 249
            +H   IK GL   V + +ALV MY+KCE ++ A ++F+S  +RN + W+AM+ G+SQNG
Sbjct: 243 QIHCITIKNGLLGFVALSNALVTMYSKCESLNEACKMFDSSGDRNSITWSAMVTGYSQNG 302

Query: 250 LVSEVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSN 309
              E ++ FS M   G +P E+T   + +AC+ + YL+ G+QLH+ ++K  F  ++F + 
Sbjct: 303 ESLEAVKLFSRMFSAGIKPSEYTIVGVLNACSDICYLEEGKQLHSFLLKLGFERHLFATT 362

Query: 310 ALVDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVP 369
           ALVDMYAK+G L +ARK F+ ++  D   W ++I GYVQ   N+EA  ++RRM + G +P
Sbjct: 363 ALVDMYAKAGCLADARKGFDCLQERDVALWTSLISGYVQNSDNEEALILYRRMKTAGIIP 422

Query: 370 DEVSLASIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSL 429
           ++                                           T ASVL+AC+ L++L
Sbjct: 423 ND------------------------------------------PTMASVLKACSSLATL 482

Query: 430 ENGQEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVG 489
           E G+++H   +  GF ++    SAL  MY+KCG ++    VF    +K+ VVSWN+MI G
Sbjct: 483 ELGKQVHGHTIKHGFGLEVPIGSALSTMYSKCGSLEDGNLVFRRTPNKD-VVSWNAMISG 542

Query: 490 LAKNGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSK 531
           L+ NG  +EALE+F +M  E + PDDVTF+ ++SACSH G V +
Sbjct: 543 LSHNGQGDEALELFEEMLAEGMEPDDVTFVNIISACSHKGFVER 542

BLAST of Lag0001481.1 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.5e-71
Identity = 152/404 (37.62%), Postives = 229/404 (56.68%), Query Frame = 0

Query: 134 WNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVHAQVI 193
           WN M+SG A+    EEA+ +F  + + G      +  SVLSA + L+ +N G+ VH+ + 
Sbjct: 120 WNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIA 179

Query: 194 KEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVSEVME 253
           K    ++VY+GSALV+MY+KC  ++ A+ VF+ + +RN+V WN+++  F QNG   E ++
Sbjct: 180 KSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALD 239

Query: 254 FFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKN-KFASNIFVSNALVDMY 313
            F  M +   +PDE T  S+ SACASL  +  G+++H  ++KN K  ++I +SNA VDMY
Sbjct: 240 VFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMY 299

Query: 314 AKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEVSLA 373
           AK   +KEAR  F+ M + + ++  ++I GY        A  MF +M     V     +A
Sbjct: 300 AKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFTKMAERNVVSWNALIA 359

Query: 374 SIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENGQEI 433
                       NE  +SL   F +L   ++    P   +FA++L+ACA L+ L  G + 
Sbjct: 360 GYTQ-----NGENEEALSL---FCLLKRESV---CPTHYSFANILKACADLAELHLGMQA 419

Query: 434 HSLILHTGFNM------DEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVG 493
           H  +L  GF        D    ++LIDMY KCG V+    VF +M  +   VSWN+MI+G
Sbjct: 420 HVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKM-MERDCVSWNAMIIG 479

Query: 494 LAKNGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSK 531
            A+NGY  EALE+F +M +    PD +T +GVLSAC HAG V +
Sbjct: 480 FAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEE 511

BLAST of Lag0001481.1 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 8.8e-67
Identity = 131/404 (32.43%), Postives = 227/404 (56.19%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVH 189
           ++++WN +I+G+   G +E+ +S F+++   G++   +TI SV +  A   +++ G  VH
Sbjct: 260 DVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVH 319

Query: 190 AQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVS 249
           +  +K   +      + L++MY+KC  +D+AK VF  + +R++V + +M+ G+++ GL  
Sbjct: 320 SIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAG 379

Query: 250 EVMEFFSCMKQFGPQPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNALV 309
           E ++ F  M++ G  PD +T T++ + CA  + LD G+++H  + +N    +IFVSNAL+
Sbjct: 380 EAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALM 439

Query: 310 DMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDEV 369
           DMYAK G+++EA   F  M+V D +SWN II GY +  Y +EA  +F  ++         
Sbjct: 440 DMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEE------- 499

Query: 370 SLASIVSACANVQSLNEATMSLSLSFAILSTYAIREYLPDQATFASVLRACAGLSSLENG 429
                                             + + PD+ T A VL ACA LS+ + G
Sbjct: 500 ----------------------------------KRFSPDERTVACVLPACASLSAFDKG 559

Query: 430 QEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVGLAK 489
           +EIH  I+  G+  D    ++L+DMYAKCG +  +  +F ++ SK+ +VSW  MI G   
Sbjct: 560 REIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKD-LVSWTVMIAGYGM 619

Query: 490 NGYAEEALEIFTQMEKESIMPDDVTFLGVLSACSHAGRVSKAER 534
           +G+ +EA+ +F QM +  I  D+++F+ +L ACSH+G V +  R
Sbjct: 620 HGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWR 621

BLAST of Lag0001481.1 vs. TAIR 10
Match: AT3G53360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.8e-65
Identity = 147/462 (31.82%), Postives = 241/462 (52.16%), Query Frame = 0

Query: 130 NIVAWNVMISGHAKRGFSEEAISFFLKLKRVGLKATRSTIGSVLSAIASLSMLNYGLMVH 189
           N+V++  +I+G+++ G   EAI  +LK+ +  L   +   GS++ A AS S +  G  +H
Sbjct: 132 NLVSYTSVITGYSQNGQGAEAIRLYLKMLQEDLVPDQFAFGSIIKACASSSDVGLGKQLH 191

Query: 190 AQVIKEGLNANVYVGSALVNMYAKCEKMDAAKEVFNSLDERNIVLWNAMLGGFSQNGLVS 249
           AQVIK   ++++   +AL+ MY +  +M  A  VF  +  ++++ W++++ GFSQ G   
Sbjct: 192 AQVIKLESSSHLIAQNALIAMYVRFNQMSDASRVFYGIPMKDLISWSSIIAGFSQLGFEF 251

Query: 250 EVMEFFSCMKQFGP-QPDEFTYTSIFSACASLQYLDFGRQLHTVMIKNKFASNIFVSNAL 309
           E +     M  FG   P+E+ + S   AC+SL   D+G Q+H + IK++ A N     +L
Sbjct: 252 EALSHLKEMLSFGVFHPNEYIFGSSLKACSSLLRPDYGSQIHGLCIKSELAGNAIAGCSL 311

Query: 310 VDMYAKSGALKEARKQFELMKVHDNVSWNAIIVGYVQEEYNDEAFFMFRRMVSNGAVPDE 369
            DMYA+ G L  AR+ F+ ++  D  SWN II G     Y DEA  +F +M S+G +PD 
Sbjct: 312 CDMYARCGFLNSARRVFDQIERPDTASWNVIIAGLANNGYADEAVSVFSQMRSSGFIPDA 371

Query: 370 VSLASIVSACANVQSLNEATM------------SLSLSFAILSTYA-------------- 429
           +SL S++ A     +L++                L++  ++L+ Y               
Sbjct: 372 ISLRSLLCAQTKPMALSQGMQIHSYIIKWGFLADLTVCNSLLTMYTFCSDLYCCFNLFED 431

Query: 430 ----------------------------------IREYLPDQATFASVLRACAGLSSLEN 489
                                             + E  PD  T  ++LR C  +SSL+ 
Sbjct: 432 FRNNADSVSWNTILTACLQHEQPVEMLRLFKLMLVSECEPDHITMGNLLRGCVEISSLKL 491

Query: 490 GQEIHSLILHTGFNMDEITCSALIDMYAKCGDVKSSIKVFHEMGSKNSVVSWNSMIVGLA 531
           G ++H   L TG   ++   + LIDMYAKCG +  + ++F  M +++ VVSW+++IVG A
Sbjct: 492 GSQVHCYSLKTGLAPEQFIKNGLIDMYAKCGSLGQARRIFDSMDNRD-VVSWSTLIVGYA 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899907.14.5e-18250.31pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Benincasa ... [more]
XP_022974928.15.5e-18048.28pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita ... [more]
XP_023534935.12.7e-17948.28pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita ... [more]
KAG6592177.11.1e-17748.28Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022935750.11.1e-17748.28pentatricopeptide repeat-containing protein At3g09040, mitochondrial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
Q9SS831.9e-10339.59Pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Arabidop... [more]
P930054.0e-7237.62Pentatricopeptide repeat-containing protein At2g33680 OS=Arabidopsis thaliana OX... [more]
Q9SIT72.2e-7037.62Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9SN391.2e-6532.43Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Q9LFI14.0e-6431.82Pentatricopeptide repeat-containing protein At3g53360, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1IHS22.7e-18048.28pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Cucurbit... [more]
A0A6J1FBI85.6e-17848.28pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Cucurbit... [more]
A0A0A0K9P11.4e-17346.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G320000 PE=4 SV=1[more]
A0A5A7TIQ61.6e-16959.30Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A5A7TIQ63.9e-3032.34Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]

Pages

Match NameE-valueIdentityDescription
AT3G09040.11.4e-10439.59Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G33680.12.8e-7337.62Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G13600.11.5e-7137.62Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18750.18.8e-6732.43Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G53360.12.8e-6531.82Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 230..278
e-value: 2.9E-12
score: 46.6
coord: 332..380
e-value: 1.1E-7
score: 31.9
coord: 476..523
e-value: 1.1E-11
score: 44.8
coord: 129..176
e-value: 1.2E-7
score: 31.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 132..163
e-value: 1.3E-4
score: 19.9
coord: 334..368
e-value: 1.9E-4
score: 19.4
coord: 446..477
e-value: 3.0E-5
score: 21.9
coord: 478..511
e-value: 7.6E-8
score: 30.1
coord: 233..267
e-value: 0.0017
score: 16.4
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 446..475
e-value: 4.0E-5
score: 23.6
coord: 306..330
e-value: 0.02
score: 15.1
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 444..474
score: 9.667924
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 476..510
score: 12.452094
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 130..164
score: 10.64348
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 332..366
score: 10.89559
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 231..265
score: 11.125777
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 40..180
e-value: 6.9E-10
score: 40.5
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 392..535
e-value: 1.7E-34
score: 121.6
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 181..282
e-value: 2.6E-23
score: 84.3
coord: 283..391
e-value: 4.2E-21
score: 77.1
NoneNo IPR availablePANTHERPTHR24015:SF1825OSJNBB0070J16.5 PROTEINcoord: 442..532
coord: 109..330
coord: 332..383
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 442..532
coord: 109..330
coord: 332..383
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 405..443
NoneNo IPR availablePANTHERPTHR24015:SF1825OSJNBB0070J16.5 PROTEINcoord: 405..443

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0001481Lag0001481gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0001481.1.exon1Lag0001481.1.exon1exon
Lag0001481.1.exon2Lag0001481.1.exon2exon
Lag0001481.1.exon3Lag0001481.1.exon3exon
Lag0001481.1.exon4Lag0001481.1.exon4exon
Lag0001481.1.exon5Lag0001481.1.exon5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0001481.1cds.Lag0001481.1CDS
cds.Lag0001481.1cds.Lag0001481.1_2CDS
cds.Lag0001481.1cds.Lag0001481.1_3CDS
cds.Lag0001481.1cds.Lag0001481.1_4CDS
cds.Lag0001481.1cds.Lag0001481.1_5CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0001481.1Lag0001481.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding