Lag0000809.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0000809.1 vs. NCBI nr
Match: KAG7990634.1 (hypothetical protein I3843_02G035100 [Carya illinoinensis])

HSP 1 Score: 399.1 bits (1024), Expect = 9.9e-107
Identity = 267/770 (34.68%), Postives = 395/770 (51.30%), Query Frame = 0

Query: 258 SRELIPLDPEIERTIHRIRRENREIIHMADQNQPEEPRPIRDYFQPVFQGQQSGIVYAPI 317
           SR++IP+DPEIERT+  +RR   +I+ MA++++   PR ++DY +PV  G  S I+  PI
Sbjct: 6   SRDIIPVDPEIERTLRSLRR--NKILAMAEEDREVLPRTLKDYVRPVVNGNYSSIMRQPI 65

Query: 318 NANNFELKTGLIQMARDCAYRGSPTEDPNSHLKSFLDI---VYSPGASQ----------- 377
           NANNFELK  LI M +   + GSP +DPN HL  FL+I   V   G ++           
Sbjct: 66  NANNFELKPALISMVQQAQFSGSPLDDPNIHLAMFLEICDTVKINGVTEDTIRLRLFPFS 125

Query: 378 ---------------CITTWDALVQAFLKKFFPPAKTVKLRTEIGTFQQQYDEQLFEAWE 437
                           I +W  + + FL KFFPPAKT +LR+EIG F+Q   E L+EAWE
Sbjct: 126 LRDKARGWLQSLQPGSIVSWQDMAERFLAKFFPPAKTAQLRSEIGQFKQNDFESLYEAWE 185

Query: 438 RFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGGTLLSKTVENARILLEDMAT 497
           R+K+L+R+CPQHG PDWLQVQ+FYNGL   T+TIVDAA+GGTL+SKT E A  LLE+MA+
Sbjct: 186 RYKDLIRRCPQHGLPDWLQVQMFYNGLNGQTRTIVDAASGGTLMSKTAEGATALLEEMAS 245

Query: 498 NSYQWPSERSTPKKIAAGVFEVDKVSALQAQMTSLANAFMKF------------------ 557
           N+YQWP+ER+  KK+ AG+ +++ ++AL AQ+ +L++                       
Sbjct: 246 NNYQWPTERTLAKKV-AGIHDLEPIAALSAQVATLSHQISALTTQRIPQSTEYLASTSMI 305

Query: 558 ----------------------------------------------------------SE 617
                                                                     S+
Sbjct: 306 VPSNEASQEQVQYVNNRNYNYRGNPMPNYYHPGLRNHENLSYGNTKNVLQPQHPPGFDSQ 365

Query: 618 SSNRTTKLEEAVIA------------------INTTVNGHSAAIKNIETHLGQLVNVVST 677
            S R   LE+A+++                  I T  +   AAIKNIE  +GQL   ++ 
Sbjct: 366 PSERKMSLEDAMVSFVQETNARFKKTDSRLDNIETHCSNMGAAIKNIEVQIGQLATTINA 425

Query: 678 MNKG--KARTE------------------------------------QEKTQMEYWEAEE 737
             +G   + TE                                    Q K ++E  E   
Sbjct: 426 QQRGAFPSNTEVNPKEQCKAITLRSGKEIERSPLKESKSTPTAVNIGQSKNKVEEDEIVN 485

Query: 738 DTSSDEAEKPN----PEPPIPSPTLMVPKEKKKKNKKKNNQVQFDKFMNAFMNMNINIPF 797
           DT  +    P       PPI +P L  P+  +K+   K    QF KF++ F  ++INIPF
Sbjct: 486 DTLEETDFAPTISFPDNPPILAPPLPYPQRFQKQKLDK----QFSKFLDIFKKIHINIPF 545

Query: 798 AEALE-MPQYNRFMKEWLAKKRKEKKVDTVYLASICSTRVQQKVPKKVADPGSFSVPCSF 827
           A+ALE MP Y +F+K+ ++KKR+ ++ +TV L+  CS  +Q+K+P+K+ DPGSF++PC+ 
Sbjct: 546 ADALEQMPNYVKFLKDIISKKRRLEEFETVKLSEECSAILQKKLPQKLKDPGSFTLPCTI 605

BLAST of Lag0000809.1 vs. NCBI nr
Match: KAG7947748.1 (hypothetical protein I3843_14G109500 [Carya illinoinensis])

HSP 1 Score: 374.4 bits (960), Expect = 2.6e-99
Identity = 243/694 (35.01%), Postives = 350/694 (50.43%), Query Frame = 0

Query: 285 MADQNQPEEPRPIRDYFQPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTED 344
           MA++++   PR ++DY +PV  G  S I+  PINANNFELK  LI M +   + GSP +D
Sbjct: 1   MAEEDREVLPRTLKDYVRPVVNGNYSSIMRQPINANNFELKPALISMVQQAQFSGSPLDD 60

Query: 345 PNSHLKSFLDI---VYSPGASQ--------------------------CITTWDALVQAF 404
           PN HL  FL+I   V   G ++                           I +W  + + F
Sbjct: 61  PNVHLAMFLEICDTVKINGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSIVSWQDMAERF 120

Query: 405 LKKFFPPAKTVKLRTEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGL 464
           L KFFPPAKT +LR+EIG F+Q   E L+EAWER+K+L+R+CPQHG PDWLQVQ+FYNGL
Sbjct: 121 LAKFFPPAKTAQLRSEIGQFKQNDFESLYEAWERYKDLIRRCPQHGLPDWLQVQMFYNGL 180

Query: 465 TPSTKTIVDAAAGGTLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSA 524
              T+TIVDAA+GGTL+SKT E A  LLE+MA+N+YQWP+ER+  KK+ AG+ E++ ++A
Sbjct: 181 NGQTRTIVDAASGGTLMSKTAEGATALLEEMASNNYQWPTERTLAKKV-AGIHELEPIAA 240

Query: 525 LQAQMTSLANAFMKF--------------------------------------------- 584
           L AQ+ +L++                                                  
Sbjct: 241 LSAQVATLSHQISALTTQRIPQSTEYVASTSMIVPSNEASQEQVQYVNNRNYNYRGNPMP 300

Query: 585 -------------------------------SESSNRTTKLEEAVIA------------- 644
                                          S+ S +   LE+A+++             
Sbjct: 301 NYYHPGLRNHENLSYGNTKNVLQPQHPPGFDSQPSEKKMSLEDAMVSFVQETNARFKKTD 360

Query: 645 -----INTTVNGHSAAIKNIETHLGQLVNVVSTMNKG----------------------- 704
                I T  +   A +KN+E  +GQL   ++   +G                       
Sbjct: 361 SRLDNIETHCSNMGATMKNLEVQIGQLATTINAQQRGAFPSNTEVNPKEQCKAITLRSGK 420

Query: 705 ---------------KARTEQEKTQMEYWEAEEDTSSDEAEKPN----PEPPIPSPTLMV 764
                           A   Q K Q+E  E   DT  +    P       PPI +P L  
Sbjct: 421 EIERAPLKESKSTPTAANNGQSKDQVEEEEIVNDTLEETDLPPTISFPDNPPILAPPLPY 480

Query: 765 PKEKKKKNKKKNNQVQFDKFMNAFMNMNINIPFAEALE-MPQYNRFMKEWLAKKRKEKKV 788
           P+  +K+   K    QF KF++ F  ++INIPFA+ALE MP Y +F+K+ ++KKR+ ++ 
Sbjct: 481 PQRFQKQKLDK----QFSKFLDIFKKIHINIPFADALEQMPNYVKFLKDIISKKRRLEEF 540

BLAST of Lag0000809.1 vs. NCBI nr
Match: KAG6734747.1 (hypothetical protein I3842_01G285500 [Carya illinoinensis])

HSP 1 Score: 369.0 bits (946), Expect = 1.1e-97
Identity = 241/694 (34.73%), Postives = 348/694 (50.14%), Query Frame = 0

Query: 285 MADQNQPEEPRPIRDYFQPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTED 344
           MA++++   PR ++DY +PV  G  S I+  PINANNFELK  LI M +   + GSP +D
Sbjct: 1   MAEEDREVLPRTLKDYVRPVVNGNYSSIMRQPINANNFELKPALISMVQQAQFSGSPLDD 60

Query: 345 PNSHLKSFLDI---VYSPGASQ--------------------------CITTWDALVQAF 404
           PN HL  FL+I   V   G ++                           I +W  + + F
Sbjct: 61  PNVHLAMFLEICDTVKINGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSIVSWQDMAERF 120

Query: 405 LKKFFPPAKTVKLRTEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGL 464
           L KFFPPAKT +LR+EIG F+Q   E L+EAWER+K+L+R+CPQHG PDWLQVQ+FYNGL
Sbjct: 121 LAKFFPPAKTAQLRSEIGQFKQNDFESLYEAWERYKDLIRRCPQHGLPDWLQVQMFYNGL 180

Query: 465 TPSTKTIVDAAAGGTLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSA 524
              T+TIVDAA+GGTL+SKT E A  LLE+MA+N+YQWP+ER+  KK+ AG+ E++ ++A
Sbjct: 181 NGQTRTIVDAASGGTLMSKTAEGATALLEEMASNNYQWPTERTLAKKV-AGIHELEPIAA 240

Query: 525 LQAQMTSLANAFMKF--------------------------------------------- 584
           L AQ+ +L++                                                  
Sbjct: 241 LSAQVATLSHQISALTTQRIPQSTEYVASTSMIVPSNEASQEQVQYVNNRNYNYRGNPMP 300

Query: 585 -------------------------------SESSNRTTKLEEAVIA------------- 644
                                          S+ S +   LE+A+++             
Sbjct: 301 NYYHPGLRNHENLSYGNTKNVLQPQHPPGFDSQPSEKKMSLEDAMVSFVQETNARFKKTD 360

Query: 645 -----INTTVNGHSAAIKNIETHLGQLVNVVSTMNKG----------------------- 704
                I T  +   A +KN+E  +GQL   ++   +G                       
Sbjct: 361 SRLDNIETHCSNMGATMKNLEVQIGQLATTINAQQRGAFPSNTEVNPKEQCKAITLRSGK 420

Query: 705 ---------------KARTEQEKTQMEYWEAEEDTSSDEAEKPN----PEPPIPSPTLMV 764
                           A   Q K Q+E  E   DT  +    P       PPI +P L  
Sbjct: 421 EIERAPLKESKSTPTAANNGQSKDQVEEEEIVNDTLEETDLPPTISFPDNPPILAPPLPY 480

Query: 765 PKEKKKKNKKKNNQVQFDKFMNAFMNMNINIPFAEALE-MPQYNRFMKEWLAKKRKEKKV 788
           P+  +K+   K    QF KF++ F  ++INIPFA+ALE MP Y +F+K+ ++KKR+ ++ 
Sbjct: 481 PQRFQKQKLDK----QFSKFLDIFKKIHINIPFADALEQMPNYVKFLKDIISKKRRLEEF 540

BLAST of Lag0000809.1 vs. NCBI nr
Match: XP_023874613.1 (uncharacterized protein LOC111987139 [Quercus suber])

HSP 1 Score: 367.5 bits (942), Expect = 3.2e-97
Identity = 240/688 (34.88%), Postives = 346/688 (50.29%), Query Frame = 0

Query: 285 MADQNQPEEPRPIRDYFQPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTED 344
           MA+  Q  +PR ++DY +P+     SGI    INANNFELK  LI M +   + GSP +D
Sbjct: 1   MAEGEQNAQPRTLKDYVRPIVNDNYSGIRRQTINANNFELKPALISMVQQAQFSGSPLDD 60

Query: 345 PNSHLKSFLDI---VYSPGASQ--------------------------CITTWDALVQAF 404
           PN HL  FL+I   +   G ++                           IT+W  + + F
Sbjct: 61  PNIHLAMFLEICDTIKMNGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSITSWQDMAEKF 120

Query: 405 LKKFFPPAKTVKLRTEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGL 464
           L KFFPPAKT +LR+EIG F+Q   E L+EAWER+K+L+R CPQHG PDWLQVQ+FYNGL
Sbjct: 121 LAKFFPPAKTAQLRSEIGQFRQNDFESLYEAWERYKDLIRCCPQHGLPDWLQVQMFYNGL 180

Query: 465 TPSTKTIVDAAAGGTLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSA 524
              T+TIVDAA+GGTL+SKT E A  LLE+MA+N+YQWP+ER+  KK+ AG+ E++  +A
Sbjct: 181 NGQTRTIVDAASGGTLMSKTAEGATSLLEEMASNNYQWPTERTMAKKV-AGIHELEPFAA 240

Query: 525 LQAQMTSLANAFMKF--------------------------------------------- 584
           L AQ+ SL++                                                  
Sbjct: 241 LSAQVASLSHQVSALTTQRIPQGAEYVAASSMTVPMNEASQEQVQYINNRNYNYRGNPMP 300

Query: 585 -----------------------------SESSNRTTKLEEAVIA--------------- 644
                                        S+ S +   LE+A+++               
Sbjct: 301 NYYHPGLRNHENFSYGNTKNVLQPPPGFDSQPSEKKMSLEDAMVSFVEETKATFKKSDSQ 360

Query: 645 ---INTTVNGHSAAIKNIETHLGQLVNVVSTMNKG--KARTE------------------ 704
              I T  +   A +KN+E  +GQL   ++   +G   + TE                  
Sbjct: 361 LDNIETHCSNMGATMKNLEVQIGQLATTINAQQRGTFPSNTEVNPKEQCKAITLRSGREI 420

Query: 705 ------------------QEKTQMEYWEAEEDTSSDEAEKPNPEPPIPSPTLMVPKEKKK 764
                             Q K ++E  E  EDT  +    P+   P   P L  P    +
Sbjct: 421 ERSPSKETETTPTAPNNGQSKNKVEEEEIVEDTLRETDMPPSISFPDNPPILSTPLPYPQ 480

Query: 765 KNKKKNNQVQFDKFMNAFMNMNINIPFAEALE-MPQYNRFMKEWLAKKRKEKKVDTVYLA 788
           + +K+    QF KF++ F  ++INIPFA+ALE MP Y +F+K+ ++KKR+ ++ +TV L+
Sbjct: 481 RFQKQKLDKQFSKFLDIFKKIHINIPFADALEQMPNYAKFLKDIISKKRRLEEFETVKLS 540

BLAST of Lag0000809.1 vs. NCBI nr
Match: XP_021279280.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC110412945 [Herrania umbratica])

HSP 1 Score: 353.2 bits (905), Expect = 6.2e-93
Identity = 250/732 (34.15%), Postives = 351/732 (47.95%), Query Frame = 0

Query: 256 RSSRELIPLDPEIERTIHRIRRENREII----HMADQNQ----------PEEPRPIRDYF 315
           R++  L+P DP+IERT  R RREN ++      MA+ N           PE  R +RDY 
Sbjct: 4   RNNLNLVPFDPDIERTFRRHRRENLQVATLNQTMAEDNNNNGNNAINLVPEANRALRDYV 63

Query: 316 QPVFQGQQSGIVYAPINANNFELKTGLIQMARDCA-YRGSPTEDPNSHLKSFLDIVYS-- 375
            P+ QG    I    INANNFE+K   IQM +    + G P++DPNSHL +FL+I  +  
Sbjct: 64  VPLVQGLHQSIRRPSINANNFEIKPAYIQMIQSSVQFSGLPSDDPNSHLVNFLEICDTFK 123

Query: 376 -----------------------------PGASQCITTWDALVQAFLKKFFPPAKTVKLR 435
                                        P  S  ITTW+ L Q FL KFFPPAKT K+R
Sbjct: 124 YNGVTDDAIRLRLFPFSLRDKAKSWLNSLPNGS--ITTWEDLAQKFLAKFFPPAKTAKMR 183

Query: 436 TEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGG 495
            +I +F Q   E L+EAWERFKELLR+CP HG PDWLQVQ FYNGL  S KTI+DAAAGG
Sbjct: 184 NDITSFIQFDGESLYEAWERFKELLRRCPHHGIPDWLQVQTFYNGLVGSIKTIIDAAAGG 243

Query: 496 TLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSALQAQMTSLANAF-- 555
            L+SK   +A  LLE+MA+N+YQWPSERS  +K A G +E+D +  L  Q+ +L+     
Sbjct: 244 ALMSKNAVDAYNLLEEMASNNYQWPSERSGSRK-AVGAYEIDALGTLTTQVAALSKKLDT 303

Query: 556 ---------------------------------------------------------MKF 615
                                                                      F
Sbjct: 304 LGVHAVQNSLVVCEMCGDSHSYDQCPYNSESVQFVGNFNRQQNNPYSNTYNPGWRNHPNF 363

Query: 616 SESSN------------------------RTTKLEEAVI----AINTTVNGHSAAIKNIE 675
           S S+N                        + ++LEE ++      +  +    A+++N+E
Sbjct: 364 SWSNNAGPSNPKPIMPPGFQQQARPQIPEKKSQLEELLLQYISKTDAIIQSQGASLRNLE 423

Query: 676 THLGQLVNVVSTMNKGKARTEQE------------------------------------- 735
           T +GQL N ++   +G   ++ +                                     
Sbjct: 424 TQVGQLANSINNRPQGSLPSDTQINPKGKEQCQAITLRSGKEIEGVNQKAVESEIEHVDK 483

Query: 736 ----KTQMEYWEAEEDTSSDEAEKPNPEPPIPSPTLMVPKEKKKKNKKKNNQVQFDKFMN 788
               + ++E  + ++D + ++       PP P P         ++ +K+  + QF KF+N
Sbjct: 484 EGMCENEIEIQQKDDDKAENQGTSQVIHPPPPFP---------QRLQKQKLEKQFQKFLN 543

BLAST of Lag0000809.1 vs. ExPASy TrEMBL
Match: A0A6J0ZX64 (LOW QUALITY PROTEIN: uncharacterized protein LOC110412945 OS=Herrania umbratica OX=108875 GN=LOC110412945 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-93
Identity = 250/732 (34.15%), Postives = 351/732 (47.95%), Query Frame = 0

Query: 256 RSSRELIPLDPEIERTIHRIRRENREII----HMADQNQ----------PEEPRPIRDYF 315
           R++  L+P DP+IERT  R RREN ++      MA+ N           PE  R +RDY 
Sbjct: 4   RNNLNLVPFDPDIERTFRRHRRENLQVATLNQTMAEDNNNNGNNAINLVPEANRALRDYV 63

Query: 316 QPVFQGQQSGIVYAPINANNFELKTGLIQMARDCA-YRGSPTEDPNSHLKSFLDIVYS-- 375
            P+ QG    I    INANNFE+K   IQM +    + G P++DPNSHL +FL+I  +  
Sbjct: 64  VPLVQGLHQSIRRPSINANNFEIKPAYIQMIQSSVQFSGLPSDDPNSHLVNFLEICDTFK 123

Query: 376 -----------------------------PGASQCITTWDALVQAFLKKFFPPAKTVKLR 435
                                        P  S  ITTW+ L Q FL KFFPPAKT K+R
Sbjct: 124 YNGVTDDAIRLRLFPFSLRDKAKSWLNSLPNGS--ITTWEDLAQKFLAKFFPPAKTAKMR 183

Query: 436 TEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGG 495
            +I +F Q   E L+EAWERFKELLR+CP HG PDWLQVQ FYNGL  S KTI+DAAAGG
Sbjct: 184 NDITSFIQFDGESLYEAWERFKELLRRCPHHGIPDWLQVQTFYNGLVGSIKTIIDAAAGG 243

Query: 496 TLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSALQAQMTSLANAF-- 555
            L+SK   +A  LLE+MA+N+YQWPSERS  +K A G +E+D +  L  Q+ +L+     
Sbjct: 244 ALMSKNAVDAYNLLEEMASNNYQWPSERSGSRK-AVGAYEIDALGTLTTQVAALSKKLDT 303

Query: 556 ---------------------------------------------------------MKF 615
                                                                      F
Sbjct: 304 LGVHAVQNSLVVCEMCGDSHSYDQCPYNSESVQFVGNFNRQQNNPYSNTYNPGWRNHPNF 363

Query: 616 SESSN------------------------RTTKLEEAVI----AINTTVNGHSAAIKNIE 675
           S S+N                        + ++LEE ++      +  +    A+++N+E
Sbjct: 364 SWSNNAGPSNPKPIMPPGFQQQARPQIPEKKSQLEELLLQYISKTDAIIQSQGASLRNLE 423

Query: 676 THLGQLVNVVSTMNKGKARTEQE------------------------------------- 735
           T +GQL N ++   +G   ++ +                                     
Sbjct: 424 TQVGQLANSINNRPQGSLPSDTQINPKGKEQCQAITLRSGKEIEGVNQKAVESEIEHVDK 483

Query: 736 ----KTQMEYWEAEEDTSSDEAEKPNPEPPIPSPTLMVPKEKKKKNKKKNNQVQFDKFMN 788
               + ++E  + ++D + ++       PP P P         ++ +K+  + QF KF+N
Sbjct: 484 EGMCENEIEIQQKDDDKAENQGTSQVIHPPPPFP---------QRLQKQKLEKQFQKFLN 543

BLAST of Lag0000809.1 vs. ExPASy TrEMBL
Match: A0A6J1DU19 (uncharacterized protein LOC111024361 OS=Momordica charantia OX=3673 GN=LOC111024361 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.1e-90
Identity = 223/557 (40.04%), Postives = 321/557 (57.63%), Query Frame = 0

Query: 297 IRDYFQPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTEDPNSHLKSFLDIV 356
           IRDY QP F     GI+  PINANN ELK GLIQM R+  +RG+ TEDPN+HL  FLD+ 
Sbjct: 27  IRDYCQPNFP-NHVGIINLPINANNSELKPGLIQMVRENTFRGNATEDPNNHLTIFLDVC 86

Query: 357 YSPGASQCITTWDA--------------LVQAFLKKFFPPAKTVKLRTEIGTFQQQYDEQ 416
            +   +  I   DA              +VQAFL  FFPPAKT +LRTEI +F++   EQ
Sbjct: 87  GTVKMNGVID--DAIRLRLFPLSLQDKEMVQAFLTNFFPPAKTTQLRTEIRSFRKYDYEQ 146

Query: 417 LFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGGTLLSKTVENARIL 476
           LFE WER+KELLRKCPQHG  +WLQ+Q+FYNGL   T+TI+DAAAGGTLLS+T ENA IL
Sbjct: 147 LFEVWERYKELLRKCPQHGNLEWLQIQMFYNGLNGQTRTILDAAAGGTLLSRTPENAYIL 206

Query: 477 LEDMATNSYQWPSERSTPKKIAAGVFEVDKVSALQAQMTSLANAFMKFS----------- 536
           L+DMA NS+QWPSERS  KK+ AG++E+D++S+L+AQ+ +L NA  K S           
Sbjct: 207 LKDMADNSFQWPSERSNAKKV-AGMYEIDELSSLKAQVQALTNAVSKLSGPGTSHSNELV 266

Query: 537 --------------------------------------ESSNRTTKLEEAVIAINTTVNG 596
                                                 E  +R +++E  V  +   + G
Sbjct: 267 AATDTYSYYEPTIEQAQFTSHPAEKKSSLEDLLGAFINECRSRASRIENQVEGMEVKLEG 326

Query: 597 HSAAIKNIETHLGQLVNVVSTMNKGKARTEQEKTQMEYWEAEEDTSSDEAEKPNPEPPIP 656
           ++ +IKN+E  +GQ+   ++TM KGK  ++ E    E+ +A    S  E ++P  +  + 
Sbjct: 327 NTTSIKNMEVQIGQIAPTLNTMQKGKFPSDIEVKPREHCKAVTLRSGKELQEPE-KKKME 386

Query: 657 SPTLMVPKEKKKKN--KKKNNQVQFDKFMNAFMNMNIN-IPFAE-ALE-MPQYNRFMKEW 716
            P +   + + K+   K+    +Q DK  ++ ++   N +P+ + ALE MP Y RFMK+ 
Sbjct: 387 EPVITTEERENKEEVVKEATPALQADKPTSSIVSSPPNSLPYPQHALEQMPNYVRFMKDI 446

Query: 717 LAKKRKEKKVDTVYLASICSTRVQQKVPKKVADPGSFSVPCSFGTYSF-RALCDLGASIN 776
           +  KRK +  +TV L   CS  +Q+K+P+K+ DPGSF++PC+  + SF +ALCD+ ASIN
Sbjct: 447 MTGKRKLEAYETVNLTEECSAILQRKLPQKLKDPGSFTIPCTISSSSFNKALCDICASIN 506

Query: 777 IIPLSLCK----KLDIGEIKSTPVKLQLADQSLVRLVGIVENVLIRVGRPFLATGRVIID 781
           ++PL + +    K+D     +  V L   + S + ++         +GR FLATG  +ID
Sbjct: 507 LMPLGVIEDVLVKVDRLIFPADFVVLXXEEDSEIPII---------LGRXFLATGXALID 566

BLAST of Lag0000809.1 vs. ExPASy TrEMBL
Match: A0A6P8DKJ2 (uncharacterized protein LOC116204231 OS=Punica granatum OX=22663 GN=LOC116204231 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.1e-82
Identity = 239/729 (32.78%), Postives = 345/729 (47.33%), Query Frame = 0

Query: 253 YCMRSSR--ELIPLDPEIERTIHRIRRENR-----EIIHMADQNQPEE----PRPIRDYF 312
           Y MR SR  EL+PLDPEIERT+HR+RRENR     +++ MAD +   +     R +RDY 
Sbjct: 105 YSMRRSRSAELLPLDPEIERTLHRLRRENRRREELQVVEMADDDINRQIQGAARALRDYA 164

Query: 313 QPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTEDPNSHLKSFL-------- 372
            P   G  S I    I ANNFELK  LIQM +   + G P E P+ H+  FL        
Sbjct: 165 VPTIMG--SAIRRPTIPANNFELKPALIQMVQSNQFGGYPNESPDEHIAGFLQYCNTVKM 224

Query: 373 -----DIV----------------YSPGASQCITTWDALVQAFLKKFFPPAKTVKLRTEI 432
                D++                ++    + ITTW  L   FL++FFPPA+T +LR EI
Sbjct: 225 NNVTDDVIRLQLFPFSLRDKARAWFNSLPQESITTWADLSSKFLRRFFPPARTARLRNEI 284

Query: 433 GTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGGTLL 492
             F +   E L+EAWERFKE +RKCP HG PD L +++FY  L  + +++VDAAAGG L+
Sbjct: 285 TNFTKFNGESLYEAWERFKEAIRKCPHHGLPDNLLIEVFYLSLDDTLRSLVDAAAGGALM 344

Query: 493 SKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVD-------KVSALQAQMTSLAN 552
            K  + A  L+E+MA++++ W +ERS  K   A V ++D       ++SAL  Q++ L +
Sbjct: 345 GKNYDEASALIEEMASSAHNWQNERS--KSRVASVNDMDTIANLTTQISALTTQVSKLTS 404

Query: 553 A--------------------------------------FMKFSESSNR----------- 612
           A                                      F+   + SN+           
Sbjct: 405 AHSFNTNQVAFCELCSGPHSTLECMSGNPSASPNGEQVNFVNNFQRSNQGPYSNTYNPGW 464

Query: 613 -----------------------------------TTKLEEAVIA----INTTVNGHSAA 672
                                               +++EE +++     +T +    A 
Sbjct: 465 RNHPNFSWRNENNALKPPPGFQKQGPAQNAPPQQSQSRMEELMLSYMQKTDTMLQNQQAT 524

Query: 673 IKNIETHLGQLVNVVSTMNKGK--ARTEQ--------------------EKTQMEYWEAE 732
           I+N+E  + Q+   +S    G   + TE+                     K Q +    E
Sbjct: 525 IRNLEGQISQISQQLSNRPSGSLPSNTEENPKGVNAIMLRSGKELEIVNRKAQTQEESPE 584

Query: 733 EDTSSDEAEKPNPE--------PPIPSPTLMVPKEKKKKNKKKNNQVQFDKFMNAFMNMN 788
           +D    + E+P  +        PP+P P  +         K++    QF KF++ F  + 
Sbjct: 585 KDKGKQKVEEPRRKSLGVKPYVPPVPFPGRL---------KQQQLDAQFAKFLDVFKKLQ 644

BLAST of Lag0000809.1 vs. ExPASy TrEMBL
Match: A0A6P8DD93 (uncharacterized protein LOC116206453 OS=Punica granatum OX=22663 GN=LOC116206453 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-81
Identity = 239/727 (32.87%), Postives = 345/727 (47.46%), Query Frame = 0

Query: 255 MRSSR--ELIPLDPEIERTIHRIRRENR-----EIIHMADQNQPEE----PRPIRDYFQP 314
           MR SR  EL+PLDPEIERT+HR+RRENR     +++ MAD +   +     R +RDY  P
Sbjct: 1   MRRSRSAELLPLDPEIERTLHRLRRENRRREELQVVEMADDDINRQIQGAARALRDYAVP 60

Query: 315 VFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTEDPNSHLKSFL---------- 374
              G  S I    I ANNFELK  LIQM +   + G P E P+ H+  FL          
Sbjct: 61  TIMG--SAIRRPTIPANNFELKPALIQMVQSNQFGGYPNESPDEHIAGFLQYCNTVKMNN 120

Query: 375 ---DIV----------------YSPGASQCITTWDALVQAFLKKFFPPAKTVKLRTEIGT 434
              D++                ++    + ITTW  L   FL++FFPPA+T +LR EI  
Sbjct: 121 VTDDVIRLQLFPFSLRDKARAWFNSLPQESITTWADLSSKFLRRFFPPARTARLRNEITN 180

Query: 435 FQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDAAAGGTLLSK 494
           F +   E L+EAWERFKE +RKCP HG PD L +++FY  L  + +++VDAAAGG L+ K
Sbjct: 181 FTKFNGESLYEAWERFKEAIRKCPHHGLPDNLLIEVFYLSLDDTLRSLVDAAAGGALMGK 240

Query: 495 TVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVD-------KVSALQAQMTSLANA- 554
             + A  L+E+MA++++ W +ERS  K   A V ++D       ++SAL  Q++ L +A 
Sbjct: 241 NYDEASALIEEMASSAHNWQNERS--KSRVASVNDMDTIANLTTQISALTTQVSKLTSAH 300

Query: 555 -------------------------------------FMKFSESSNR------------- 614
                                                F+   + SN+             
Sbjct: 301 SFNTNQVAFCELCSGPHSTLECMSGNPSASPNGEQVNFVNNFQRSNQGPYSNTYNPGWRN 360

Query: 615 ---------------------------------TTKLEEAVIA----INTTVNGHSAAIK 674
                                             +++EE +++     +T +    A I+
Sbjct: 361 HPNFSWRNENNALKPPPGFQKQGPAQNAPPQQSQSRMEELMLSYMQKTDTMLQNQQATIR 420

Query: 675 NIETHLGQLVNVVSTMNKGK--ARTEQ--------------------EKTQMEYWEAEED 734
           N+E  + Q+   +S    G   + TE+                     K Q +    E+D
Sbjct: 421 NLEGQISQISQQLSNRPSGSLPSNTEENPKGVNAIMLRSGKELEIVNRKAQTQEESPEKD 480

Query: 735 TSSDEAEKPNPE--------PPIPSPTLMVPKEKKKKNKKKNNQVQFDKFMNAFMNMNIN 788
               + E+P  +        PP+P P         ++ K++    QF KF++ F  + IN
Sbjct: 481 KGKQKVEEPRQKSLGVKPYVPPVPFP---------RRLKQQQLDAQFAKFLDVFKKLQIN 540

BLAST of Lag0000809.1 vs. ExPASy TrEMBL
Match: A0A6P6XAQ1 (Reverse transcriptase OS=Coffea arabica OX=13443 GN=LOC113740608 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 3.2e-79
Identity = 217/646 (33.59%), Postives = 304/646 (47.06%), Query Frame = 0

Query: 295 RPIRDYFQPVFQGQQSGIVYAPINANNFELKTGLIQMARDCAYRGSPTEDPNSHLKSFLD 354
           R +RD+  P  QG Q+ IV   +NANNFE+K  LIQM +   Y G+ TEDPNSHL +FL+
Sbjct: 9   RILRDFALPGAQGSQTSIVRPTVNANNFEIKPSLIQMVQQSQYGGNATEDPNSHLSTFLE 68

Query: 355 I---VYSPGASQ--------------------------CITTWDALVQAFLKKFFPPAKT 414
           I   +   G S+                            TTWD L +AFL KFFPP KT
Sbjct: 69  ICDTIKFNGVSEDAIKLRLFPFSLRDKAKVWLQSHPPNTFTTWDELAKAFLNKFFPPGKT 128

Query: 415 VKLRTEIGTFQQQYDEQLFEAWERFKELLRKCPQHGYPDWLQVQLFYNGLTPSTKTIVDA 474
            KLR +I +F QQ  E L+EAWER++EL R+CP HG PDWL VQ FYNGLT  TKT VDA
Sbjct: 129 AKLRMDITSFSQQEGETLYEAWERYRELQRRCPHHGLPDWLVVQTFYNGLTYPTKTHVDA 188

Query: 475 AAGGTLLSKTVENARILLEDMATNSYQWPSERSTPKKIAAGVFEVDKVSALQAQMTSLAN 534
           AAGG L+ KT E A+ L+E+MA N+YQW +ER   ++  AG+ EVD ++ L A+M ++  
Sbjct: 189 AAGGALMGKTAEEAQQLIEEMAANNYQWANERGNSRR-TAGMLEVDTLNMLSAKMDNVVK 248

Query: 535 ------------------------------------------------------------ 594
                                                                       
Sbjct: 249 MLNRQVGSSSNQGVVVASCTICGGDHDDFMCSSSEQVQYLNNYNRPPQNNPYSNTYNPGW 308

Query: 595 --------------------------------------AFMKFSESSN-RTTKLEEAVIA 654
                                                 A  K + +SN +  KL  A   
Sbjct: 309 RNHPNFGWKDQGNQQRPVNPPGFQQKQTLHESKPAWELAIEKLANASNDKIEKLASATTQ 368

Query: 655 INTTVNGHSAAI----KNIETHLGQLVNVVSTMNKGKARTEQEKTQMEYWEAEEDTSSDE 714
               + G    +    +N+E  LGQ+ N V+  N+G   ++ E    E+ +A    S  E
Sbjct: 369 RFERIEGRMDQLTNMYRNVEVQLGQIANAVNNRNQGDLPSKTEVNPREHVKAITLRSGKE 428

Query: 715 AEKPNPEPPIPSPTLMVPKEKKKKNKKKNNQVQ---FDKFMNAFMNMNINIPFAEALEMP 774
             +P   P + S      +E KK ++ K    +    +K     + M    P    +  P
Sbjct: 429 LVEP---PVVGSGREFEKRENKKLSELKEGSKEEKGKEKIEENELQMEDATPIPPPI--P 488

Query: 775 QYNRFMKEWLAKKRKEKKVDTVYLASICSTRVQQKVPKKVADPGSFSVPCSFGTYSF-RA 781
            Y +F+KE + KKRK    +T+ L   CS  +Q K+P K+ DPGSF+VPC+ G   F +A
Sbjct: 489 SYAKFLKEIMTKKRKLVDSETIALTEECSAIIQNKLPPKLKDPGSFTVPCTIGNVEFSKA 548