IVF0026896.2 (mRNA) Melon (IVF77) v1

Overview
NameIVF0026896.2
TypemRNA
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionurease accessory protein F
Locationchr05: 22795440 .. 22798015 (+)
Sequence length1587
RNA-Seq ExpressionIVF0026896.2
SyntenyIVF0026896.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCGTCTTATTATGCACCCATGTTAGTACTCAGCTCTCTCTCATTGACTTGCTTTGTCGAATTTTGGATGGTTAAATAAGAAAATTGCAAAATTAAGGAGCTTCCTCACATCATATGGAAATGGCGGATTTCAATTTTCATTCCCATTAAGGTTAATTCGTTTCCATTCTTCACATTTTTGTTCGTTTTGTTCTTCTTCTTTGTGAAAGAAAGCTGTGCTATGCTCTAGGGTTCTTATCTTGATTCTTGGCTGTTAACGTTACTACTTTTGTTTTCATTGTCATTCATCCAATTTCATTTCGATTCTTGCATATTCAGTTATAGTTCCAACACTCAATTCTCTCGCGCTAATATGCTATCCTGATGTCAATGTACCACGAGCATTGTTCAACGTTTTGCTTCTCCTTTCGCGAGATGATGTTTTTAGTGGTTGCCCATTACAATGTTGATCGTCTTGGTCTTGGTTTAAGAAACAGTTAAATAACTCTTGTGTTTTTGAAATCTTGACTTTGATCAGGTTTTTGAGTTGGTTTTAATCAAACAGGAAATGCATGGTTGGTTAGATTAGTTTTGGTTACTCTGGAGTTCAGTCTCAAAACCTTGTTGGATTCTCTATTTCTCTTTGGACTTCAGTTTTAAGGGCTTTTGAAATTATTCGTTAGGTTTCATTTTACTTGATGATTGGACTTCCGTCTTGTAGTTGACTTTCTCCGTTTTCTTTGTGCTTCGGTTTTTAGGCGTTTTGAAATTATTCGTTAGGTTTTTTTTACTTGATTGGACCTTCGTCTTGTAGTTAACTTCCTTTTCGTGGCTTTACCATTTTGTATGTTTGTGTATTTTTTCTTCTCTCTTTTCTGAATGAAAATCAAATTTCTTAAACAAAAAAGAAACGATTGTGGATTCAGATTGTTTTGACCTCGAGTAGAGATGTGTAATTTATTGATTCTTGATGAACCCTTCGTGTATATATAAAGCATTTCTTGGTGACCCTTTGTATATGTATAGCATTTCTTGGTGAGCCCTTTGTTATCACTTGTTAGATAAATTTGAAGTTAGCTACTTAAAACTCCGAATCTGTTCTATCCATTTTCTAGATAGGTAACTTGAATGCATTTGTGTTCTCTTGTTTCTGTTTTGCAGAATCTATGCAATAGTACAAACTTGACATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGAAGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTTATTCAGCCACACTATCACCTGATTTTGAAACCTGGAAGAAAAACGACAGGTTATTGGATGCAATATTAACTAATGAAGTTAGTCGAAAGGCATCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATTGTGTTTTCCGAAATCTCATCTCTGAAAGCAATGAGGGAGCACTTGTACGGGACTGGGACTGTGTCGTTCCACCATGCTCCTATCTTCGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTCTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACGAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCACAAGTTGCTGAAAACGTATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAGCGGTTCCTTTGTTAGAGACGGTACAGGGATGCCATTCGTACCTGTTTTCTAGAATGTTTTGTTCTTGAAAAGTTTTTCAAGTGAAAACTGCATCTTTCTTTTGATTTTGGGTATTGGAGTCCCCTTCTTTCATGTTCTCTGCTTGAAAATGGTTGTGGATTATGAGATAATAGTGGCAATGTTGAAATTCTCTTACTCACATATATGCATTTCATTTTGTATGGCACTCCATGTTTTTGTTCCATCGAAGACTTTGTTTGTCTATGGCCATGATTCAATATCAAGTAAATGTAAATAGAGTTTTTAAGTACTTCGAAATCTTGATTATTCTGATCCTTTCAATTCTTATCGGTCCATGCAACATAAAATACTTTCTTTATGGATTTCTTTACTCTAAATACACAATTCTTTTCATTCATGTGGCACGTAGTTTCGTGGACAATTTTCTCATGCCAAAATATTTCCTACCTTAAGCTTGTCATGAAGAAAAGATCAAACATTTGTCTAGCATCTTCTCGAGCACATGAAAAACAGTTTAACATCTTAACATTGGCTGATAGAAAATTTCTCCCACCTTAAGTAAGAGAAATCCAAATCTCTAACAAAAATATAAACCATACTTCGATCCATTTTAGCTTGAAATGTTTATAAATTTTAGAGTATTTGAATGGAGAGATGCTAGAACTTGAAAGAAGTTGATCTTGATAGAGAGATTTAAGAGAATGTACAGAAAAGAAAGAGTAAAATGAAAAAGAAAATTGGGGTTACTGTACTTCCTTGGGGAGTTTAC

mRNA sequence

AATCGTCTTATTATGCACCCATGTTAGTACTCAGCTCTCTCTCATTGACTTGCTTTGTCGAATTTTGGATGGTTAAATAAGAAAATTGCAAAATTAAGGAGCTTCCTCACATCATATGGAAATGGCGGATTTCAATTTTCATTCCCATTAAGAATCTATGCAATAGTACAAACTTGACATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGAAGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTTATTCAGCCACACTATCACCTGATTTTGAAACCTGGAAGAAAAACGACAGGTTATTGGATGCAATATTAACTAATGAAGTTAGTCGAAAGGCATCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATTGTGTTTTCCGAAATCTCATCTCTGAAAGCAATGAGGGAGCACTTGTACGGGACTGGGACTGTGTCGTTCCACCATGCTCCTATCTTCGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTCTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACGAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCACAAGTTGCTGAAAACGTATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAGCGGTTCCTTTGTTAGAGACGGTACAGGGATGCCATTCGTACCTGTTTTCTAGAATGTTTTGTTCTTGAAAAGTTTTTCAAGTGAAAACTGCATCTTTCTTTTGATTTTGGGTATTGGAGTCCCCTTCTTTCATGTTCTCTGCTTGAAAATGGTTGTGGATTATGAGATAATAGTGGCAATGTTGAAATTCTCTTACTCACATATATGCATTTCATTTTGTATGGCACTCCATGTTTTTGTTCCATCGAAGACTTTGTTTGTCTATGGCCATGATTCAATATCAAGTAAATGTAAATAGAGTTTTTAAGTACTTCGAAATCTTGATTATTCTGATCCTTTCAATTCTTATCGGTCCATGCAACATAAAATACTTTCTTTATGGATTTCTTTACTCTAAATACACAATTCTTTTCATTCATGTGGCACGTAGTTTCGTGGACAATTTTCTCATGCCAAAATATTTCCTACCTTAAGCTTGTCATGAAGAAAAGATCAAACATTTGTCTAGCATCTTCTCGAGCACATGAAAAACAGTTTAACATCTTAACATTGGCTGATAGAAAATTTCTCCCACCTTAAGTAAGAGAAATCCAAATCTCTAACAAAAATATAAACCATACTTCGATCCATTTTAGCTTGAAATGTTTATAAATTTTAGAGTATTTGAATGGAGAGATGCTAGAACTTGAAAGAAGTTGATCTTGATAGAGAGATTTAAGAGAATGTACAGAAAAGAAAGAGTAAAATGAAAAAGAAAATTGGGGTTACTGTACTTCCTTGGGGAGTTTAC

Coding sequence (CDS)

ATGGATGACAAACATTGTCACTGGAGCCAATGGCAACTGCTCGATTCTATCCTTCCAACTGGTGGCTTCGCTCATTCATTCGGCCTTGAGGCTGCAATACAAGCTCAAATCGTCTCAAGTCCAGAAGATCTCAAAACTTTTGTGATCCATTTATTGGACAACACTGGAAGTTTGTTTCTTCCCTTTGTTTATTCAGCCACACTATCACCTGATTTTGAAACCTGGAAGAAAAACGACAGGTTATTGGATGCAATATTAACTAATGAAGTTAGTCGAAAGGCATCTGTTACGCAAGGGTCAGCACTCATGAGAGTTTCAGCAATTGTGTTTTCCGAAATCTCATCTCTGAAAGCAATGAGGGAGCACTTGTACGGGACTGGGACTGTGTCGTTCCACCATGCTCCTATCTTCGGGCTAATCTGCGGTCTACTTGGATGGGATGGCACAATGTCACAAAGAGCATATCTCTTTATTACCTTGAGAGATGTTATTTCTGCTGCAACGAGATTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAACATCAGCTTGCACAAGTTGCTGAAAACGTATTGAAGAGATGGATGAACCGTCCTGTTGAAGAAGCTTGCCAAGCGGTTCCTTTGTTAGAGACGGTACAGGGATGCCATTCGTACCTGTTTTCTAGAATGTTTTGTTCTTGA

Protein sequence

MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFLPFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS
Homology
BLAST of IVF0026896.2 vs. ExPASy Swiss-Prot
Match: Q9XHZ3 (Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 4.9e-88
Identity = 154/221 (69.68%), Postives = 185/221 (83.71%), Query Frame = 0

Query: 8   WSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFLPFVYSAT 67
           WSQWQLLDSILPTGGFAHSFGLEAAIQ ++VSSPEDL+T +IH+LDNT SL LPFVYSA 
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  LSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREHLYGTG 127
            SPD ETW K D +L+A LTN+VS KAS++QGSAL R++A VF+E+ +LK +R+   G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 TVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAQ 187
            V FHHAPIFGL+CGLLG D   SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A 
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           V E VL++WMNR   EACQ  PLL+ VQGCH YLFSR+FCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

BLAST of IVF0026896.2 vs. ExPASy Swiss-Prot
Match: E0ZS46 (Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.9e-84
Identity = 146/226 (64.60%), Postives = 194/226 (85.84%), Query Frame = 0

Query: 3   DKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFLPF 62
           ++H  WSQWQLLDSILPTGGFAHS+GLEAA+Q+++V++PE+L++FV+ +L+NTGSL LPF
Sbjct: 36  NQHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPF 95

Query: 63  VYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREH 122
           V  A  SPD  TW K D+LL+A+LTNEVSRKAS++QGSAL+RV+A VF+EI SL+ +R+ 
Sbjct: 96  VCCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQT 155

Query: 123 LYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+  VSFHHAPIFGLICGL+G+D   +QRAY+F+T+RDVISAATRLNL+GPL A+VLQ
Sbjct: 156 FLGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQ 215

Query: 183 HQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           HQ+A+ AE ++++W +R VEEA Q  PLL+ +QGCH+Y+FSR+FC+
Sbjct: 216 HQVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

BLAST of IVF0026896.2 vs. ExPASy Swiss-Prot
Match: Q0E3L5 (Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2 SV=2)

HSP 1 Score: 313.5 bits (802), Expect = 1.9e-84
Identity = 146/226 (64.60%), Postives = 194/226 (85.84%), Query Frame = 0

Query: 3   DKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFLPF 62
           ++H  WSQWQLLDSILPTGGFAHS+GLEAA+Q+++V++PE+L++FV+ +L+NTGSL LPF
Sbjct: 36  NQHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPF 95

Query: 63  VYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREH 122
           V  A  SPD  TW K D+LL+A+LTNEVSRKAS++QGSAL+RV+A VF+EI SL+ +R+ 
Sbjct: 96  VCCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQT 155

Query: 123 LYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+  VSFHHAPIFGLICGL+G+D   +QRAY+F+T+RDVISAATRLNL+GPL A+VLQ
Sbjct: 156 FLGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQ 215

Query: 183 HQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           HQ+A+ AE ++++W +R VEEA Q  PLL+ +QGCH+Y+FSR+FC+
Sbjct: 216 HQVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

BLAST of IVF0026896.2 vs. ExPASy Swiss-Prot
Match: B7K911 (Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=ureF PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.0e-13
Identity = 62/221 (28.05%), Postives = 104/221 (47.06%), Query Frame = 0

Query: 12  QLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDN----TGSLFLPFVYSAT 71
           QL DS  P+G F  S GLEA  Q + + S +DL+ F+  LL N    T  + L   Y  +
Sbjct: 14  QLSDSFFPSGSFTLSHGLEALAQGEQIHSIKDLEIFLQILLHNKLGTTDLVALIHAYRGS 73

Query: 72  LSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREHLYGTG 131
              D +  ++ D  L A    E +R+     G AL+ V+   + + + L+ + +     G
Sbjct: 74  KREDLDAVREADHQLFAQTLIEKNREMGRKSGRALLMVARETWQD-TQLETLEKET-AKG 133

Query: 132 TVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAQ 191
           T++  H  IF ++  + G   T +  A+L   +  ++ AA RLN++G L A  +  +LA 
Sbjct: 134 TINGLHPIIFAVVGRVAGLSETDTGLAFLHSFITGLLGAAIRLNIIGHLQAQKILLKLAP 193

Query: 192 VAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
             E   ++     + E     PL++  Q  H  L  R+F +
Sbjct: 194 DLETTYQKATEINLNEMFSCTPLIDIAQMNHQNLDYRLFAN 232

BLAST of IVF0026896.2 vs. ExPASy Swiss-Prot
Match: Q117Z4 (Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=ureF PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.7e-12
Identity = 57/221 (25.79%), Postives = 99/221 (44.80%), Query Frame = 0

Query: 12  QLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDN----TGSLFLPFVYSAT 71
           QL DS  PTG F  S GLE  +Q   + S  ++  F+  LL N    +  + L   Y   
Sbjct: 14  QLSDSFFPTGSFTFSHGLETLVQTGKIQSQPEILYFLQILLRNKVGVSDVVALIHSYRGC 73

Query: 72  LSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREHLYGTG 131
            + D E  +  DR+L        +R+     G AL+ V++  + +   LK +      +G
Sbjct: 74  KNGDIEAVRVADRMLFVQTAIAKNRETQRQSGRALLMVASSTWQD-ERLKTLNIDAV-SG 133

Query: 132 TVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAQ 191
            +   H  IFG +  ++G D   +  A+L   + +++ AA RL ++G + A  +  QL  
Sbjct: 134 NIHCLHPVIFGAVTSVVGLDERNAVLAFLHGLVTNILGAAIRLGILGHIQAQQILLQLVP 193

Query: 192 VAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
             E V    ++  +E+     P ++  Q  H  L  ++F +
Sbjct: 194 DIEAVWSTAVSMNLEQMWSCTPFIDIAQMQHPKLAHKLFAN 232

BLAST of IVF0026896.2 vs. ExPASy TrEMBL
Match: A0A0A0LJ08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 214/228 (93.86%), Postives = 221/228 (96.93%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSP+DLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+SAT SPDFETWKKND LLDA+LTNEVSRKASVTQGSALMRVSAIVFSEI SLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E+LYGTG VSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           LQHQLA VAE++LKRWMNRPVEEACQ VPLLETVQGCHS LFS+MFCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

BLAST of IVF0026896.2 vs. ExPASy TrEMBL
Match: A0A5D3B9D0 (Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001170 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 4.0e-117
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 215
           LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 214

BLAST of IVF0026896.2 vs. ExPASy TrEMBL
Match: A0A5A7TYF8 (Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001330 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.4e-116
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK CHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKRCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 215
           LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETV 214

BLAST of IVF0026896.2 vs. ExPASy TrEMBL
Match: A0A1S3BWP6 (urease accessory protein F OS=Cucumis melo OX=3656 GN=LOC103494267 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 9.8e-116
Identity = 212/213 (99.53%), Postives = 212/213 (99.53%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK CHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKRCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET 214
           LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET 213

BLAST of IVF0026896.2 vs. ExPASy TrEMBL
Match: A0A6J1G3M3 (urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.6e-112
Identity = 204/228 (89.47%), Postives = 213/228 (93.42%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS PEDLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+S TLSPD ETW+KNDR L+AILTNEVSRKASVTQGSALMRV+AIVFSEISSLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTG VSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           LQHQLA VAE+ LK+WMNRPVEEACQ VPLLETVQGCH+YLFSR+FCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

BLAST of IVF0026896.2 vs. NCBI nr
Match: XP_004144755.1 (urease accessory protein F [Cucumis sativus] >KGN60994.1 hypothetical protein Csa_021347 [Cucumis sativus])

HSP 1 Score: 436 bits (1120), Expect = 1.04e-153
Identity = 214/228 (93.86%), Postives = 221/228 (96.93%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSP+DLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+SAT SPDFETWKKND LLDA+LTNEVSRKASVTQGSALMRVSAIVFSEI SLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E+LYGTG VSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 228
           LQHQLA VAE++LKRWMNRPVEEACQ VPLLETVQGCHS LFS+MFCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

BLAST of IVF0026896.2 vs. NCBI nr
Match: XP_008453620.1 (PREDICTED: urease accessory protein F [Cucumis melo])

HSP 1 Score: 426 bits (1096), Expect = 2.76e-150
Identity = 212/213 (99.53%), Postives = 212/213 (99.53%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK CHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL
Sbjct: 1   MDDKRCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET 213
           LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET 213

BLAST of IVF0026896.2 vs. NCBI nr
Match: XP_022946416.1 (urease accessory protein F [Cucurbita moschata])

HSP 1 Score: 413 bits (1062), Expect = 9.27e-145
Identity = 204/228 (89.47%), Postives = 213/228 (93.42%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS PEDLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+S TLSPD ETW+KNDR L+AILTNEVSRKASVTQGSALMRV+AIVFSEISSLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTG VSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 228
           LQHQLA VAE+ LK+WMNRPVEEACQ VPLLETVQGCH+YLFSR+FCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

BLAST of IVF0026896.2 vs. NCBI nr
Match: XP_023545319.1 (urease accessory protein F [Cucurbita pepo subsp. pepo])

HSP 1 Score: 413 bits (1061), Expect = 1.63e-144
Identity = 204/228 (89.47%), Postives = 213/228 (93.42%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS PEDLKTFVIHLLDNTGSL L
Sbjct: 14  MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 73

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+S TLSPD ETW+KNDR L+AILTNEVSRKASVTQGSALMRV+AIVFSEISSLKAMR
Sbjct: 74  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 133

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTG VSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 134 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 193

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 228
           LQHQLA VAE+ LK+WMNRPVEEACQ VPLLETVQGCH+YLFSR+FCS
Sbjct: 194 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 241

BLAST of IVF0026896.2 vs. NCBI nr
Match: XP_022999571.1 (urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory protein F [Cucurbita maxima])

HSP 1 Score: 412 bits (1059), Expect = 2.66e-144
Identity = 203/228 (89.04%), Postives = 212/228 (92.98%), Query Frame = 0

Query: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60
           MDDK  HWSQWQLLDSILPTGGFAHSFGLEAAIQA+IVS PEDLKTFVIHLLDNTGSL L
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120
           PFV+S TLSPD ETW+KNDR L+AILTNEVSRKAS+TQGSALMRV+AIVFSEISSLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASITQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTG VSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 228
           LQHQLA VAE  LK+WMNRPVEEACQ VPLLETVQGCH+YLFSR+FCS
Sbjct: 188 LQHQLALVAEGTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

BLAST of IVF0026896.2 vs. TAIR 10
Match: AT1G21840.1 (urease accessory protein F )

HSP 1 Score: 325.5 bits (833), Expect = 3.5e-89
Identity = 154/221 (69.68%), Postives = 185/221 (83.71%), Query Frame = 0

Query: 8   WSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFLPFVYSAT 67
           WSQWQLLDSILPTGGFAHSFGLEAAIQ ++VSSPEDL+T +IH+LDNT SL LPFVYSA 
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  LSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMREHLYGTG 127
            SPD ETW K D +L+A LTN+VS KAS++QGSAL R++A VF+E+ +LK +R+   G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 TVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAQ 187
            V FHHAPIFGL+CGLLG D   SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A 
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAENVLKRWMNRPVEEACQAVPLLETVQGCHSYLFSRMFCS 229
           V E VL++WMNR   EACQ  PLL+ VQGCH YLFSR+FCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XHZ34.9e-8869.68Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1[more]
E0ZS461.9e-8464.60Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 S... [more]
Q0E3L51.9e-8464.60Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2... [more]
B7K9111.0e-1328.05Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=653... [more]
Q117Z49.7e-1225.79Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203... [more]
Match NameE-valueIdentityDescription
A0A0A0LJ081.2e-11893.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1[more]
A0A5D3B9D04.0e-117100.00Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7TYF83.4e-11699.53Lon protease-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3BWP69.8e-11699.53urease accessory protein F OS=Cucumis melo OX=3656 GN=LOC103494267 PE=4 SV=1[more]
A0A6J1G3M36.6e-11289.47urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_004144755.11.04e-15393.86urease accessory protein F [Cucumis sativus] >KGN60994.1 hypothetical protein Cs... [more]
XP_008453620.12.76e-15099.53PREDICTED: urease accessory protein F [Cucumis melo][more]
XP_022946416.19.27e-14589.47urease accessory protein F [Cucurbita moschata][more]
XP_023545319.11.63e-14489.47urease accessory protein F [Cucurbita pepo subsp. pepo][more]
XP_022999571.12.66e-14489.04urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory p... [more]
Match NameE-valueIdentityDescription
AT1G21840.13.5e-8969.68urease accessory protein F [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002639Urease accessory protein UreFPFAMPF01730UreFcoord: 38..185
e-value: 9.9E-18
score: 65.0
IPR002639Urease accessory protein UreFPIRSFPIRSF009467Urease_acces_UreFcoord: 3..228
e-value: 5.3E-48
score: 161.7
IPR038277UreF domain superfamilyGENE3D1.10.4190.10Urease accessory protein UreFcoord: 3..207
e-value: 1.2E-49
score: 171.1
NoneNo IPR availablePANTHERPTHR33620UREASE ACCESSORY PROTEIN Fcoord: 1..228

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
IVF0026896IVF0026896gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026896.2-five_prime_utrIVF0026896.2-five_prime_utr-chr05:22795440..22795591five_prime_UTR
IVF0026896.2-five_prime_utrIVF0026896.2-five_prime_utr-chr05:22796581..22796606five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026896.2-exonIVF0026896.2-exon-chr05:22795440..22795591exon
IVF0026896.2-exonIVF0026896.2-exon-chr05:22796581..22798015exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026896.2-cdsIVF0026896.2-cds-chr05:22796607..22797293CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026896.2-three_prime_utrIVF0026896.2-three_prime_utr-chr05:22797294..22798015three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
IVF0026896.2IVF0026896.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0030163 protein catabolic process
biological_process GO:0006508 proteolysis
molecular_function GO:0016151 nickel cation binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0004252 serine-type endopeptidase activity