IVF0026576.1 (mRNA) Melon (IVF77) v1

Overview
NameIVF0026576.1
TypemRNA
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionDNA polymerase epsilon subunit
Locationchr12: 25610908 .. 25615136 (+)
Sequence length1575
RNA-Seq ExpressionIVF0026576.1
SyntenyIVF0026576.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGCTTTACAGCATCAAACTGGAAGCTCTTGACGAGATCGTTTCCTTTGTCTCTCGTTTCCATGGCTTCGAAGATGACGCTATAGAACTTGTTCTTGACGATCTCCATGAAGAATCTTGTACTGTTTCTTCTTCTTTCATACTACATTTATATCTTCTCTGCAATTCACCTCATTCCTTTTCATGGACAGTTTCTTTGTTTTGTCTATTATGGCAGTGAAATCTCCAATATTAGAAAAGGATGGGGTGCATCGGGTTATCAGCAAACTGCTTGCAGCTGAAGAAGCGGAAGAAGCAAGTCCCAATACCATTACCAGTACAACTGCTCTTTGCATCATCGACGCTTTGATATCTCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCATCGGTATGGTTTTCTCCCATTTATATTTATTTTTAATAGTTGTTAAACTAAGCATTCCTGCTTAAGCATATACCTTGGGCCCAGAAGTCAGAGCAAAATCTCTCATTCCCATGTGTTGTTGAATACTTGAACTAAAAAAAACTACTAGTTGGATTTTTGGGATAATTAGACGCGGATAACTGATGGAGCTCGTTGCAAAAAAGAGAGATATAATGGATTTAACTGAGAACTATCGTCGTCAACATTCCTTTTCTCTGCACTTGAAATCACCTGTTCTTGAACCTTGTATACATATATATTTTTAGGCACACAGGGGACCTTCCTATTCATGGAGATGCTCCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTGTCCCAAAGGCTCTCCCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGGTGCTCCATTTCTCTTGACCCATGAATTGATCATGTTAGCCTTTACAATTGAACATGAGCATTTACACTAATTTTGTTTGTGATGTGTTTCCCAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGGAAATGGGTCATGGGAGTGATCTCTCAAATGGAAGATGGCCATTTTTACTTGGAAGACCTTACTGCATCTGTGGAAATTAATTTATCTAATGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAGTCTGAACTTGTTTGATATTTGAAGATGACATGCCCTGCAGTTTTCATTAATAATTAGCACAAGATAACTACAGGTCTATTTACAGAGAACACCATTATCGTAGCTGAAGGAGAGATGCTCGTGGAGGGAATTTTCCAGGTTTGTACCACGTTTGCCTTCAAGTTCTGTATAAATAGGTGGTTGGAATTTCCCTTAGTGTTCTATTGGGTATTTCTAACCCTCCTTGTTTTCAGAGAGAACTTGCTTTCTTAAAGACATATGTAGTGTTAATTATGCTATTTCTCTACGGATCATCCAGTTTTCCTATTGGCTATTGACAGGATGTACTTTGCTTACGAAGTTTACGTAGTTTATCAGATTATTATGCTTGGTTACGTTTTTAAAAAGTATATCTTTGCATATTTTCCTATATTCTTTTCAAAAAAAACTTTGCAGTTTTGGTACTTTTAATGTATAGCAGTACTTTACTATAACTTCTTCCTAATTTGAATGCTTTTTGAAGTTTTTACATGCTTATGAGATGATTTTTTACAATGATATGGGTTGGGTCTTAGTTCTGTTTGGTCCTGGTCAGAGTTTTGCATGCCTAAGTTATATTTCAAGTAGTTTTCAATTGGTAAGTGGATAACACCATCTTTTAGCTGGTTATTTAATATTTCGTTAGGTATCCAGAAATTGAAAGTTTACTTTTGAGAGATATGAAACCTCATTTTTCTAAACAAATCTTTATTTTATTTTTTTGAGCGAGTTCCGCCTACACTCTTCTCAAAAGGTTTTGAATCTTTGATAACATGAGGTTGACATACAAGCACAACACAGTTAAGGATGAAGATGCAAATGTCCTGCCTTCATCAAAAGCATGTTTACTATTCAAAATTGGATTTGCTTGTGGGATTTCATTCTTGTCCTTTACTTTCTTATTGTTTTCTACCTAGCACAAGGATACCTTCCTGCTCAAACGTTTTGGAAAATGTCCAGAACTTGTTTCATAGAAGTGTTTCAAATACTCTTCGTTGTAGTAATAATGTGCTTTGCCTGGCCTCTATTTAGGTTGTTACGTGTGGATTTCCTCCATTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGTGCTACCTAAAGAGGAGACTGTATCCTCCTGTTTCGGTCTATAAAATGAATTTCTAAATAAGCATTGGAAAGGAGGGCTATAGTTGACATTTTATTGTGCTGAATTTTGGCTATTAAAGGGGTGAATACACTTACATACCGTGCAATCTACTTGTTTTCCTTAATCATCAATAGCTCAGGCTTGCAGATCTGGAAAAGAAAGCTGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGTGACAAATAACCTTTTTATATTTCATGTGTATTGTGTGTATATGAAGCTTGGTGGCCTTATTGAAACTTTTTCATGTGGTAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTGGAAGTGGTTCCTTCCTTATTTGTTTTGATGGGGAATTTTTGTTCCCATCCTTGTAATATTGCCTTTAATTCTTTCTCAAGTCTCAGGTATGTATTATTGCAATTGTTTTCTGTGTTATTGAGATGAGTTCAGTGCAATAATTAGTTCAAATGTGCAGACTACAGTTTGGTAAGTTAGGAAAAATGATAGCAGCTCATCCACGATTAAACAAGCATAGCAAGTTTCTTTTTATTCCCGGTCCAGATGATGCAGGTTTTGTTTTGTTATCTGTTAAGAAACTTCTCTCCTTGAGTTCAACTATGCATGATATTTTCTGAGTTTTTGTTTACATGGAGTTTATAGGGCCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACCGAAGAGCTTCAAGTGCATGTTCCAAATGCCACTTTCTCAAGTAACCCTTGCAGGTGAATACTTCGATTGCTGACTTGTAGCCTTAATAATGGTGCTTCTAATCTTAATTATAGTATTCTGTTGCATGAGGGGCTTCACTTTTACAGCTTACTCGTGAAATAAATCATCTTTTTCTATTTGGAATAACCCAACAGAACTGTATTTAATAGATTTAAAGGCTAGTGTTAATGGTATGGATTTAGTTATCCAGTTTTTTTTTTTTTAACTATTGAATTTAGAGGGAAAAAGGTCATTTATTAACAAGAGAGAACAAAGCTATGATGGACAAAATAATTGGTCTTCTAAAAGAGCGTTTGATTTTTTAATTAATTAAGAGGAATAAATCGATTTTAAGAGGACATTATGCTCCCTTTGGTGAATGCTATACCTTTTCCGTTTTTACTCCTTAGTGGTGGATAATTGTCATAATTGAATGCAGGGTCAGATTCTACACTCAGGAAATTGTATTTTTCCGACAGGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCCACAGAAGAAACAAGTGATCCTTTTGAGCATGTGACTTTTTTTCTTTTCCTTTTATATTTCTGTTATTTTTTTTCTAGTTCTCTGTTTATGGATTCTAACCCTTTCGGATATTTATATACGGAGGCCTTAAGGTTTCATGATTCTCTTGCAGCTTGTTGCCACCATAACTCATCAAAGTCATCTCTGTCCTCTTCCTCTAGTTATTCAACCAATTATCTGGAATTACGACCACTGTCTTCATCTATATCCTACTCCTCATGTGGTACTTACCCATCTCTCCATCTAGTGTTTTAACGATCTATTTATACATCCTAAGTTGGATGTCCTTTCTCTCCACACCTGGAGACAGAAAATGAATGAAGGTTCTGATGAATGATAAGTGTTCTACTGCGATAGAATCTCAATGTTGCATGAACTTTCAGATAATTATATCCATCTTTATTTGGTTTGTGCAGATAGTTTTGGGGGATAGAAGTAAGCAACAAGCGTTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTCGTGGCGTATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAA

mRNA sequence

ATGGACGCATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGCTTTACAGCATCAAACTGGAAGCTCTTGACGAGATCGTTTCCTTTGTCTCTCGTTTCCATGGCTTCGAAGATGACGCTATAGAACTTGTTCTTGACGATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGAAAAGGATGGGGTGCATCGGGTTATCAGCAAACTGCTTGCAGCTGAAGAAGCGGAAGAAGCAAGTCCCAATACCATTACCAGTACAACTGCTCTTTGCATCATCGACGCTTTGATATCTCCAAGGGACCTTCCTATTCATGGAGATGCTCCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTGTCCCAAAGGCTCTCCCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGGAAATGGGTCATGGGAGTGATCTCTCAAATGGAAGATGGCCATTTTTACTTGGAAGACCTTACTGCATCTGTGGAAATTAATTTATCTAATGCTATATCCTTTACTGGCAACTTCTATGATCACAAGATAACTACAGGTCTATTTACAGAGAACACCATTATCGTAGCTGAAGGAGAGATGCTCGTGGAGGGAATTTTCCAGGTTGTTACGTGTGGATTTCCTCCATTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGTGCTACCTAAAGAGGAGACTCTCAGGCTTGCAGATCTGGAAAAGAAAGCTGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTGGAAGTGGTTCCTTCCTTATTTGTTTTGATGGGGAATTTTTGTTCCCATCCTTGTAATATTGCCTTTAATTCTTTCTCAAGTCTCAGACTACAGTTTGGTAAGTTAGGAAAAATGATAGCAGCTCATCCACGATTAAACAAGCATAGCAAGTTTCTTTTTATTCCCGGTCCAGATGATGCAGGGCCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACCGAAGAGCTTCAAGTGCATGTTCCAAATGCCACTTTCTCAAGTAACCCTTGCAGGGTCAGATTCTACACTCAGGAAATTGTATTTTTCCGACAGGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCCACAGAAGAAACAAGTGATCCTTTTGAGCATCTTGTTGCCACCATAACTCATCAAAGTCATCTCTGTCCTCTTCCTCTAGTTATTCAACCAATTATCTGGAATTACGACCACTGTCTTCATCTATATCCTACTCCTCATGTGATAGTTTTGGGGGATAGAAGTAAGCAACAAGCGTTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTCGTGGCGTATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAA

Coding sequence (CDS)

ATGGACGCATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGCTTTACAGCATCAAACTGGAAGCTCTTGACGAGATCGTTTCCTTTGTCTCTCGTTTCCATGGCTTCGAAGATGACGCTATAGAACTTGTTCTTGACGATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGAAAAGGATGGGGTGCATCGGGTTATCAGCAAACTGCTTGCAGCTGAAGAAGCGGAAGAAGCAAGTCCCAATACCATTACCAGTACAACTGCTCTTTGCATCATCGACGCTTTGATATCTCCAAGGGACCTTCCTATTCATGGAGATGCTCCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTGTCCCAAAGGCTCTCCCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGGAAATGGGTCATGGGAGTGATCTCTCAAATGGAAGATGGCCATTTTTACTTGGAAGACCTTACTGCATCTGTGGAAATTAATTTATCTAATGCTATATCCTTTACTGGCAACTTCTATGATCACAAGATAACTACAGGTCTATTTACAGAGAACACCATTATCGTAGCTGAAGGAGAGATGCTCGTGGAGGGAATTTTCCAGGTTGTTACGTGTGGATTTCCTCCATTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGTGCTACCTAAAGAGGAGACTCTCAGGCTTGCAGATCTGGAAAAGAAAGCTGTTAACGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTGGAAGTGGTTCCTTCCTTATTTGTTTTGATGGGGAATTTTTGTTCCCATCCTTGTAATATTGCCTTTAATTCTTTCTCAAGTCTCAGACTACAGTTTGGTAAGTTAGGAAAAATGATAGCAGCTCATCCACGATTAAACAAGCATAGCAAGTTTCTTTTTATTCCCGGTCCAGATGATGCAGGGCCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACCGAAGAGCTTCAAGTGCATGTTCCAAATGCCACTTTCTCAAGTAACCCTTGCAGGGTCAGATTCTACACTCAGGAAATTGTATTTTTCCGACAGGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCCACAGAAGAAACAAGTGATCCTTTTGAGCATCTTGTTGCCACCATAACTCATCAAAGTCATCTCTGTCCTCTTCCTCTAGTTATTCAACCAATTATCTGGAATTACGACCACTGTCTTCATCTATATCCTACTCCTCATGTGATAGTTTTGGGGGATAGAAGTAAGCAACAAGCGTTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTCGTGGCGTATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAA

Protein sequence

MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSPILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPRDLPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Homology
BLAST of IVF0026576.1 vs. ExPASy Swiss-Prot
Match: Q500V9 (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 7.3e-204
Identity = 357/534 (66.85%), Postives = 425/534 (79.59%), Query Frame = 0

Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFED-DAIELVLDDLHEESLKSPILEKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +F   +D +AI+L+LD+L +E+ KS  ++ +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  GVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------DLPIHG 126
            V  +I++LL A  A E      TS ++L IIDA + P+                LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPE---EPTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +A AK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLVEGI 246
           VISQ+EDGHFYLEDL+ASVEI+LS A          KITTG FTENTII+AEGEM V GI
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKA----------KITTGFFTENTIILAEGEMQVNGI 247

Query: 247 FQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWL 306
           FQV+TCGFPPLE+RDK+LK  +  DFFGGG L KEE ++LADLE++AVND FVILSDIWL
Sbjct: 248 FQVITCGFPPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWL 307

Query: 307 DSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAA 366
           D EE M KLET+LDGFE+VE VPSLFV MGNFCS PCN++F S+SSLR QFGKLG+MI  
Sbjct: 308 DDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGN 367

Query: 367 HPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQE 426
           HPRL ++S+FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNA FSSNPCRV+FY QE
Sbjct: 368 HPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQE 427

Query: 427 IVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCL 486
           IVFFRQDLLYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L
Sbjct: 428 IVFFRQDLLYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHAL 487

Query: 487 HLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
            LYPTPH IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 488 RLYPTPHTIVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

BLAST of IVF0026576.1 vs. ExPASy Swiss-Prot
Match: Q54Y85 (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.2e-78
Identity = 178/533 (33.40%), Postives = 295/533 (55.35%), Query Frame = 0

Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSPILEK-D 66
           +K++ +   + G +SIK +A+  I++ +    G++   I  ++D+L +   KS  L+  +
Sbjct: 6   KKQITKSFSLTG-FSIKADAMKCIINRIKN-EGYD---ITQLIDNLLKNLDKSQYLQTIE 65

Query: 67  GVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR----------------DLPIH 126
           G  ++I  + +++  E+          AL +IDA  +P                 D  +H
Sbjct: 66  GCFKLIDTINSSDAIEK---------EALKVIDAFNTPLFNFDSNSKQFIKSENFDRSLH 125

Query: 127 GDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVM 186
           G + +K+ LYR R+L + QR  R+ +FS P             I+P+ SL+G TGRK V+
Sbjct: 126 GSSNSKSDLYRKRYLKVLQRTERNDYFSTPVLASDKLKNEYQTITPLSSLLGNTGRKHVL 185

Query: 187 GVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLVEG 246
           G ISQ+E+  FY+EDL  +V+I++S A          K   G+ T N+II A GE  ++G
Sbjct: 186 GTISQIEEDQFYIEDLNTNVKIDISKA----------KFEFGIVTINSIIQASGE-FIDG 245

Query: 247 IFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETL-RLADLEKKAVNDMFVILSDI 306
           +F       PP EER ++LK L   D F  G  P+++T+ +L   EK+  ++  + LSD+
Sbjct: 246 VFIADKIQLPPTEERCETLKFLQNIDMF--GERPQKKTMEQLIKYEKEKEDNSILFLSDV 305

Query: 307 WLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMI 366
           WLDSE  M +L+ +  G++  +  P   +LMGNF  HP  +   +   L+  F +L  +I
Sbjct: 306 WLDSERVMERLDYLFGGYK--DCPPFAMILMGNFTEHP--LINGTQYQLKKYFNQLAMVI 365

Query: 367 AAHPRLNKHSKFLFIPGPDDAGPS--TVLPRCALPKYLTEELQVHVPNATFSSNPCRVRF 426
             +P +++ ++F+F+PGP D   S   +LP+  +     ++    +P +TF++NPCR+R+
Sbjct: 366 QKYPNIHQFTQFIFVPGPTDPTGSLLNILPKFPISNVFIKDFISLIPKSTFTTNPCRIRY 425

Query: 427 YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY 486
            +QEI+ FR DL  +MRR C++ PS  ++ D  +HL+  I   SHLC L L  +PI WNY
Sbjct: 426 CSQEIIIFRDDLTNKMRRHCILEPS--QSCDISQHLIELICSNSHLCNLTLEDKPIYWNY 485

Query: 487 DHCLHLYPTPHVIVLGDRSKQ-QAFKYTGITCFNPGSFTNDSTFVAYRPCNQE 519
           DH + +YP P ++V+GD+S Q +  +  G    NP SF+ D +F  Y P N++
Sbjct: 486 DHAMSVYPLPDLLVIGDKSNQYEHSRADGTYSMNPSSFSTDYSFAHYIPANKQ 505

BLAST of IVF0026576.1 vs. ExPASy Swiss-Prot
Match: Q5ZKQ6 (DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.4e-74
Identity = 176/547 (32.18%), Postives = 290/547 (53.02%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFE-DDAIELVLDDLHEESLKS 60
           M+   LR+++    ++RGL  ++ EA+  +   +   +  E +D I+ ++D + ++ L +
Sbjct: 1   MEPERLRRRLGSAFRVRGLL-LRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLST 60

Query: 61  PILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISP----------RDLPI- 120
            ++E   V   + +   ++  +E   N      A  I   + +P           +LP+ 
Sbjct: 61  NMIELSTVEAAVQE--CSQALDETIKNVFNIIGAFDIPRYIYNPDRKRFVPLSMTNLPVP 120

Query: 121 --HGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAF----DIGMSHFGSCEISPIQSLVGQ 180
              G A  KA L+R+R+ +L QR  R + F+  A     D   S F   ++  +++L+G 
Sbjct: 121 NLFGTARDKAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKF---QLKTVETLLGN 180

Query: 181 TGR---KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTII 240
           T +     V+G+I+Q+++G ++LED T  V+++LS A          +  +GL+TE+  +
Sbjct: 181 TAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKA----------QFHSGLYTESCFV 240

Query: 241 VAEGEMLVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGG-GVLPKEETLRLADLEKKAV 300
           +AEG    + +F V   GFPP E    +       +FFGG      + + +L  LE +  
Sbjct: 241 LAEG-WYEDEVFHVNAFGFPPTEPSATTRAFYGNINFFGGPSSASVKASAKLKQLEDENE 300

Query: 301 NDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLR 360
           + MFV LSD+WLD  E + KL T+  G+ +    P+ F   GNF S P     N   SL+
Sbjct: 301 DAMFVFLSDVWLDQAEVLEKLHTMFSGYSSAP--PTCFFFCGNFSSAP--YGKNQIQSLK 360

Query: 361 LQFGKLGKMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFS 420
                L  +I  +P ++K S+F+F+PGP+D GP +VLPR  L + +T+E +  VP + F+
Sbjct: 361 GSLKALADIICEYPSIHKSSRFVFVPGPEDPGPGSVLPRPPLAENITQEFRQLVPFSVFT 420

Query: 421 SNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLV 480
           +NPCRV++ TQEI+ FR+DL+ +M R+C+  PS+  + D   H V TI  Q HL PLPL 
Sbjct: 421 TNPCRVQYCTQEIIIFREDLVNKMCRNCVRFPSS--SMDIPNHFVKTILSQGHLTPLPLY 480

Query: 481 IQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQ 525
           + P+ W YD+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+
Sbjct: 481 VSPVYWAYDYALRVYPVPDMLVIADKYDPFTVTNTDCLCINPGSFPRSGFSFKVFYPSNK 524

BLAST of IVF0026576.1 vs. ExPASy Swiss-Prot
Match: A7YWS7 (DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.0e-72
Identity = 169/539 (31.35%), Postives = 284/539 (52.69%), Query Frame = 0

Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFE-DDAIELVLDDLHEESLKSPILEK 65
           LR +     K+RGL  ++ EA+  +   +   +  E +DA+E ++D + ++ L S ++E+
Sbjct: 6   LRSRALSAFKLRGL-MLRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSNMIER 65

Query: 66  DGVHRVISKLLAAEEAEEA------------SPNTITSTTALCIIDALISPRDLP-IHGD 125
             V   + +   ++  +E              P  + ++     +  L++    P + G 
Sbjct: 66  SVVEAAVQE--CSQSVDETIDHIFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLFGT 125

Query: 126 APAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT---GRKW 185
           A  KA L+R+R+ +L QR  R + F+ P         GS  ++  I++L+G T   G   
Sbjct: 126 ARDKAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTSKIGDVI 185

Query: 186 VMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLV 245
           V+G+I+Q+++G F+LED T +++++LS A          +  +GL+TE+  ++AEG    
Sbjct: 186 VLGMITQLKEGKFFLEDPTGTIQLDLSKA----------QFHSGLYTESCFVLAEG-WFE 245

Query: 246 EGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEET-LRLADLEKKAVNDMFVILS 305
           + +F V   GFPP E    +       +FFGG      +T  +L  LE +  + MFV +S
Sbjct: 246 DQVFHVNAFGFPPTEPSSTTRAYYGNTNFFGGPSNTSVKTSAKLKQLEDENKDAMFVFIS 305

Query: 306 DIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGK 365
           D+WLD  E + KL  +  G+      P+ F+L GNF S P     N   +L+     L  
Sbjct: 306 DVWLDQVEVLEKLHIMFSGYS--PAPPTCFILCGNFSSAP--YGKNQVQALKDSLKTLAD 365

Query: 366 MIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRF 425
           +I  +P +++ S+F+F+PGP+D G  ++LPR    + +T E +  VP + F++NPCR+++
Sbjct: 366 IICGYPNIHQSSRFVFVPGPEDPGFGSILPRPPFAESITNEFRQRVPFSVFTTNPCRIQY 425

Query: 426 YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY 485
            TQEI+ FR+DL+ +M R+C+  PS+    D   H V T+  Q HL PLPL + P+ W Y
Sbjct: 426 CTQEIIVFREDLVNKMCRNCVRFPSS--NLDIPNHFVKTVLSQGHLTPLPLYVCPVYWAY 485

Query: 486 DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 525
           D+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+ VE S L
Sbjct: 486 DYALRVYPVPDLLVIADKYDPFTLTNTECLCINPGSFPRSGFSFKVFYPSNKVVEDSKL 524

BLAST of IVF0026576.1 vs. ExPASy Swiss-Prot
Match: P56282 (DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 6.2e-70
Identity = 168/539 (31.17%), Postives = 280/539 (51.95%), Query Frame = 0

Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFE-DDAIELVLDDLHEESLKSPILEK 65
           LR +     K+RGL  ++ EA+  +   +      E +D +E +++ + ++ L S ++E+
Sbjct: 6   LRSRALSAFKLRGLL-LRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIER 65

Query: 66  DGVHRVISKLLAAEEAEEA------------SPNTITSTTALCIIDALISPRDLP-IHGD 125
             V   + +   ++  +E              P  + ++     +  L++    P + G 
Sbjct: 66  SVVEAAVQE--CSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLFGT 125

Query: 126 APAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT---GRKW 185
              KA ++R+R+ +L QR  R + F+ P         GS  ++  I++L+G T   G   
Sbjct: 126 PRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAI 185

Query: 186 VMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLV 245
           V+G+I+Q+++G F+LED T +V+++LS A          +  +GL+TE   ++AEG    
Sbjct: 186 VLGMITQLKEGKFFLEDPTGTVQLDLSKA----------QFHSGLYTEACFVLAEG-WFE 245

Query: 246 EGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEET-LRLADLEKKAVNDMFVILS 305
           + +F V   GFPP E    +       +FFGG      +T  +L  LE++  + MFV LS
Sbjct: 246 DQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLS 305

Query: 306 DIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGK 365
           D+WLD  E + KL  +  G+      P+ F+L GNF S P     N   +L+     L  
Sbjct: 306 DVWLDQVEVLEKLRIMFAGYS--PAPPTCFILCGNFSSAP--YGKNQVQALKDSLKTLAD 365

Query: 366 MIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRF 425
           +I  +P +++ S+F+F+PGP+D G  ++LPR  L + +T E +  VP + F++NPCR+++
Sbjct: 366 IICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQY 425

Query: 426 YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY 485
            TQEI  FR+DL+ +M R+C+  PS+        H V TI  Q HL PLPL + P+ W Y
Sbjct: 426 CTQEITVFREDLVNKMCRNCVRFPSSNLAIP--NHFVKTILSQGHLTPLPLYVCPVYWAY 485

Query: 486 DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 525
           D+ L +YP P ++V+ D+        T   C NPGSF     +F  + P N+ VE S L
Sbjct: 486 DYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKL 524

BLAST of IVF0026576.1 vs. ExPASy TrEMBL
Match: A0A5D3BIF9 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00380 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.1e-301
Identity = 519/539 (96.29%), Postives = 520/539 (96.47%), Query Frame = 0

Query: 1    MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
            MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 613  MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 672

Query: 61   ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
            ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 673  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 732

Query: 121  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
            LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 733  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 792

Query: 181  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
            KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM
Sbjct: 793  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 852

Query: 241  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
            LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 853  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 912

Query: 301  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
            SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 913  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 972

Query: 361  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
            KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 973  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 1032

Query: 421  FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
            FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 1033 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 1092

Query: 481  YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
            YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1093 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1151

BLAST of IVF0026576.1 vs. ExPASy TrEMBL
Match: A0A5A7T342 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00330 PE=3 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 6.9e-298
Identity = 517/539 (95.92%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1    MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
            MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 614  MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 673

Query: 61   ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
            ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 674  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 733

Query: 121  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
            LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 734  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 793

Query: 181  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
            KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYD  ITTGLFTENTIIVAEGEM
Sbjct: 794  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYD--ITTGLFTENTIIVAEGEM 853

Query: 241  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
            LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 854  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 913

Query: 301  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
            SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 914  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 973

Query: 361  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
            KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 974  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 1033

Query: 421  FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
            FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 1034 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 1093

Query: 481  YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
            YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1094 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1150

BLAST of IVF0026576.1 vs. ExPASy TrEMBL
Match: A0A1S4DTQ5 (DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.1e-291
Identity = 508/539 (94.25%), Postives = 509/539 (94.43%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
           ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120

Query: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
           KWVMGVISQMEDGHFYLEDLTASVEINLSNA          KITTGLFTENTIIVAEGEM
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNA----------KITTGLFTENTIIVAEGEM 240

Query: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
           LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300

Query: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
           SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360

Query: 361 KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
           KMIAAHPRLNKHSKF FIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 361 KMIAAHPRLNKHSKFXFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420

Query: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
           FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480

Query: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
           YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529

BLAST of IVF0026576.1 vs. ExPASy TrEMBL
Match: A0A6J1GCV3 (DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.8e-264
Identity = 461/539 (85.53%), Postives = 486/539 (90.17%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MD  TLR+KVQRK+KIRG YSIKL+AL EIVSF SRF G EDDAI+LVLD LH+ESL+S 
Sbjct: 1   MDESTLRRKVQRKSKIRG-YSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSS 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAL---------------ISPRD 120
           I++KD VHRV+S LLAA+EA E  P+T TST+ALCIIDA                +    
Sbjct: 61  IIDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGS 120

Query: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD GMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
           KWVMGVISQMEDGHF+LEDLTASVEINLS+A          KITTGLFTENTI+VAEGEM
Sbjct: 181 KWVMGVISQMEDGHFFLEDLTASVEINLSSA----------KITTGLFTENTIVVAEGEM 240

Query: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
           LVEGIF+V+TCGFPPLE+RDKSLKLLAGQDFFGGG L KEETLRLADLEK+AVNDMFVIL
Sbjct: 241 LVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVIL 300

Query: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
           SDIWLDSEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNS+SSLRLQFGKLG
Sbjct: 301 SDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLG 360

Query: 361 KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
           KMIAA PRL +HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVR
Sbjct: 361 KMIAARPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVR 420

Query: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
           FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480

Query: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
           YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of IVF0026576.1 vs. ExPASy TrEMBL
Match: A0A6J1KGF0 (DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 5.0e-264
Identity = 459/539 (85.16%), Postives = 486/539 (90.17%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MD  TLR+KVQRK+KIRG YSIKL+AL EIVSF SRF G EDDAI+LVLD LH+ESL+S 
Sbjct: 1   MDESTLRRKVQRKSKIRG-YSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSS 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAL---------------ISPRD 120
           I++KD VHRV+S LLAA+EA E  P+T TST+ALCIIDA                +    
Sbjct: 61  IIDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYMHTGS 120

Query: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFD GMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDSGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
           KWVMGVISQMEDGHFYLEDLTASVEINLS+A          KITTGLFTENT++VAEGEM
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSSA----------KITTGLFTENTVVVAEGEM 240

Query: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
           LVEGIF+V+TCGFPPLE+RDKSLKLLAGQDFFGGG L KEETLRLADLEK+A+NDMFVIL
Sbjct: 241 LVEGIFKVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVIL 300

Query: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
           SDIWLDSEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNS+SSLRLQFGKLG
Sbjct: 301 SDIWLDSEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLG 360

Query: 361 KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
           KMIA+ PRL +HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVR
Sbjct: 361 KMIASRPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVR 420

Query: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
           FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480

Query: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
           YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of IVF0026576.1 vs. NCBI nr
Match: TYJ98844.1 (DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 519/539 (96.29%), Postives = 520/539 (96.47%), Query Frame = 0

Query: 1    MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
            MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 613  MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 672

Query: 61   ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
            ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 673  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 732

Query: 121  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
            LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 733  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 792

Query: 181  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
            KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM
Sbjct: 793  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 852

Query: 241  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
            LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 853  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 912

Query: 301  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
            SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 913  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 972

Query: 361  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
            KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 973  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 1032

Query: 421  FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
            FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 1033 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 1092

Query: 481  YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 524
            YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1093 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1151

BLAST of IVF0026576.1 vs. NCBI nr
Match: KAA0036041.1 (DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1026 bits (2652), Expect = 0.0
Identity = 517/539 (95.92%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1    MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
            MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 614  MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 673

Query: 61   ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
            ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 674  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 733

Query: 121  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
            LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 734  LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 793

Query: 181  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
            KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYD  ITTGLFTENTIIVAEGEM
Sbjct: 794  KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYD--ITTGLFTENTIIVAEGEM 853

Query: 241  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
            LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 854  LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 913

Query: 301  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
            SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 914  SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 973

Query: 361  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
            KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 974  KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 1033

Query: 421  FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
            FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 1034 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 1093

Query: 481  YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 524
            YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1094 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1150

BLAST of IVF0026576.1 vs. NCBI nr
Match: XP_016899382.1 (PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 508/539 (94.25%), Postives = 509/539 (94.43%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------D 120
           ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDA    +               D
Sbjct: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120

Query: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
           KWVMGVISQMEDGHFYLEDLTASVEINLSNA          KITTGLFTENTIIVAEGEM
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNA----------KITTGLFTENTIIVAEGEM 240

Query: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
           LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL
Sbjct: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300

Query: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
           SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG
Sbjct: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360

Query: 361 KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
           KMIAAHPRLNKHSKF FIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR
Sbjct: 361 KMIAAHPRLNKHSKFXFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420

Query: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
           FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480

Query: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 524
           YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529

BLAST of IVF0026576.1 vs. NCBI nr
Match: XP_011657177.1 (DNA polymerase epsilon subunit B [Cucumis sativus] >KAE8653182.1 hypothetical protein Csa_020045 [Cucumis sativus])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 483/538 (89.78%), Postives = 497/538 (92.38%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MD LTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSR HGFED+AIE VLD+LHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR--------------DL 120
           IL+KD VHRVIS ++AA++  E SPNTITST+ALCII+A   P+              +L
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEML 240
           WVMGVISQMEDGHFYLEDLTASVEINLS+A          KITTGLFTENTIIVAEGEML
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSA----------KITTGLFTENTIIVAEGEML 240

Query: 241 VEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILS 300
           VEGIFQVVTCGFPPLEER KSLKLLAGQDFFG GVLPKEETLR ADLEKKAVNDMFVILS
Sbjct: 241 VEGIFQVVTCGFPPLEERGKSLKLLAGQDFFGDGVLPKEETLRRADLEKKAVNDMFVILS 300

Query: 301 DIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGK 360
           DIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGK
Sbjct: 301 DIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGK 360

Query: 361 MIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRF 420
           MIAAHPRLN+HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HV NA FSSNPCRVRF
Sbjct: 361 MIAAHPRLNEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVSNAIFSSNPCRVRF 420

Query: 421 YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY 480
           YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY
Sbjct: 421 YTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNY 480

Query: 481 DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 524
           DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 DHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

BLAST of IVF0026576.1 vs. NCBI nr
Match: XP_038894268.1 (DNA polymerase epsilon subunit B [Benincasa hispida])

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 480/539 (89.05%), Postives = 495/539 (91.84%), Query Frame = 0

Query: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60
           MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHG EDDAI+LVLD+LH+ESLKS 
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGCEDDAIDLVLDNLHDESLKSS 60

Query: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAL---------------ISPRD 120
           IL+KD VHRV+S +LAA+E  E SPNTITST+ALCIIDA                +    
Sbjct: 61  ILDKDAVHRVVSIMLAADEVGEESPNTITSTSALCIIDAFDISKFRYDPIKKIFYLHTGK 120

Query: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFD GMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDTGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEM 240
           KWVMGVISQMEDGHFYLEDLTASVEINLSNA          KITTGLFTENTI+VAEGEM
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNA----------KITTGLFTENTIVVAEGEM 240

Query: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVIL 300
           LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGG L KEETLRLADLEKKAVNDMFVIL
Sbjct: 241 LVEGIFQVVTCGFPPLEERDKSLKLLAGQDFFGGGALSKEETLRLADLEKKAVNDMFVIL 300

Query: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLG 360
           SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCS PCN+AFNSFSSLRLQFGKLG
Sbjct: 301 SDIWLDSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLG 360

Query: 361 KMIAAHPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVR 420
           +MIAAHPRL +HSKFLFIPGPDDAGPSTVLPRCALPK+LTEELQ+HVPNA FSSNPCRVR
Sbjct: 361 RMIAAHPRLIEHSKFLFIPGPDDAGPSTVLPRCALPKFLTEELQMHVPNAIFSSNPCRVR 420

Query: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480
           FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN
Sbjct: 421 FYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWN 480

Query: 481 YDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 524
           YDHCLHLYPTPHVIVL DRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 481 YDHCLHLYPTPHVIVLADRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529

BLAST of IVF0026576.1 vs. TAIR 10
Match: AT5G22110.1 (DNA polymerase epsilon subunit B2 )

HSP 1 Score: 711.4 bits (1835), Expect = 5.2e-205
Identity = 357/534 (66.85%), Postives = 425/534 (79.59%), Query Frame = 0

Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFED-DAIELVLDDLHEESLKSPILEKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +F   +D +AI+L+LD+L +E+ KS  ++ +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  GVHRVISKLLAAEEAEEASPNTITSTTALCIIDALISPR---------------DLPIHG 126
            V  +I++LL A  A E      TS ++L IIDA + P+                LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPE---EPTTSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +A AK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLVEGI 246
           VISQ+EDGHFYLEDL+ASVEI+LS A          KITTG FTENTII+AEGEM V GI
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKA----------KITTGFFTENTIILAEGEMQVNGI 247

Query: 247 FQVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWL 306
           FQV+TCGFPPLE+RDK+LK  +  DFFGGG L KEE ++LADLE++AVND FVILSDIWL
Sbjct: 248 FQVITCGFPPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWL 307

Query: 307 DSEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAA 366
           D EE M KLET+LDGFE+VE VPSLFV MGNFCS PCN++F S+SSLR QFGKLG+MI  
Sbjct: 308 DDEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGN 367

Query: 367 HPRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQE 426
           HPRL ++S+FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNA FSSNPCRV+FY QE
Sbjct: 368 HPRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQE 427

Query: 427 IVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCL 486
           IVFFRQDLLYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L
Sbjct: 428 IVFFRQDLLYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHAL 487

Query: 487 HLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 525
            LYPTPH IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 488 RLYPTPHTIVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q500V97.3e-20466.85DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV... [more]
Q54Y851.2e-7833.40DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum OX=44689 GN=pole2 P... [more]
Q5ZKQ65.4e-7432.18DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1[more]
A7YWS71.0e-7231.35DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1[more]
P562826.2e-7031.17DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3BIF95.1e-30196.29Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7T3426.9e-29895.92Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S4DTQ52.1e-29194.25DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1[more]
A0A6J1GCV33.8e-26485.53DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=... [more]
A0A6J1KGF05.0e-26485.16DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 ... [more]
Match NameE-valueIdentityDescription
TYJ98844.10.096.29DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa][more]
KAA0036041.10.095.92DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa][more]
XP_016899382.10.094.25PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo][more]
XP_011657177.10.089.78DNA polymerase epsilon subunit B [Cucumis sativus] >KAE8653182.1 hypothetical pr... [more]
XP_038894268.10.089.05DNA polymerase epsilon subunit B [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G22110.15.2e-20566.85DNA polymerase epsilon subunit B2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016266DNA polymerase epsilon, subunit BPIRSFPIRSF000799DNA_pol_epsilon_2coord: 1..524
e-value: 1.8E-174
score: 578.8
IPR016266DNA polymerase epsilon, subunit BPANTHERPTHR12708DNA POLYMERASE EPSILON SUBUNIT Bcoord: 7..523
IPR007185DNA polymerase alpha/delta/epsilon, subunit BPFAMPF04042DNA_pol_E_Bcoord: 282..489
e-value: 6.5E-47
score: 159.6
NoneNo IPR availableGENE3D1.10.8.60coord: 1..73
e-value: 8.4E-8
score: 34.2
NoneNo IPR availableGENE3D3.60.21.60coord: 291..520
e-value: 2.6E-6
score: 28.5

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
IVF0026576IVF0026576gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026576.1-exonIVF0026576.1-exon-chr12:25610908..25611076exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25611175..25611315exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25611662..25611807exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25611903..25612061exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25612128..25612208exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25613121..25613225exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25613380..25613460exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25613544..25613674exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25613746..25613840exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25613931..25614030exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25614456..25614570exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25614692..25614802exon
IVF0026576.1-exonIVF0026576.1-exon-chr12:25614996..25615136exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25610908..25611076CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25611175..25611315CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25611662..25611807CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25611903..25612061CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25612128..25612208CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25613121..25613225CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25613380..25613460CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25613544..25613674CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25613746..25613840CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25613931..25614030CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25614456..25614570CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25614692..25614802CDS
IVF0026576.1-cdsIVF0026576.1-cds-chr12:25614996..25615136CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
IVF0026576.1IVF0026576.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006261 DNA-dependent DNA replication
biological_process GO:0006260 DNA replication
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0006536 glutamate metabolic process
biological_process GO:0051781 positive regulation of cell division
cellular_component GO:0008622 epsilon DNA polymerase complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0070182 DNA polymerase binding
molecular_function GO:0004351 glutamate decarboxylase activity
molecular_function GO:0030170 pyridoxal phosphate binding