IVF0018496.2 (mRNA) Melon (IVF77) v1

Overview


Name	IVF0018496.2
Type	mRNA
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Pyruvate kinase family protein
Location	chr06: 199888 .. 200143 (-)
Sequence length	150
RNA-Seq Expression	IVF0018496.2
Synteny	IVF0018496.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGGTATGGTTGGCTTCCTTAGCAACTCTCCCTTTGTTTCTAGTGGGTAATGCGTTTGAGTTAAAGTAGGGAACTTTTGTTTTTCTATTCTTTCTTTGGTGGCTGCTAGTTGCTCGGTTTGACTTTTCATGGGGTAGTCTGGATTACCATCAAGAGACTTTGGAAAATTTGAAGATTGCTGTTAAAAGCACCAAGAAACTTTGTGCTGTATGTTCGTTTTTAATATGTTTGAGTGTATATTTTATCATTTGA

mRNA sequence

ATGTCGGTTGCTCGGTTTGACTTTTCATGGGGTAGTCTGGATTACCATCAAGAGACTTTGGAAAATTTGAAGATTGCTGTTAAAAGCACCAAGAAACTTTGTGCTGTATGTTCGTTTTTAATATGTTTGAGTGTATATTTTATCATTTGA

Coding sequence (CDS)

ATGTCGGTTGCTCGGTTTGACTTTTCATGGGGTAGTCTGGATTACCATCAAGAGACTTTGGAAAATTTGAAGATTGCTGTTAAAAGCACCAAGAAACTTTGTGCTGTATGTTCGTTTTTAATATGTTTGAGTGTATATTTTATCATTTGA

Protein sequence

MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAVCSFLICLSVYFII

Homology

[Close]

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot
Match: B8BM17 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.6e-12
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  +YHQETLENLK+A+KSTKKLCAV
Sbjct: 54 MSVARFDFSWGDAEYHQETLENLKVAIKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot
Match: Q2QXR8 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0145700 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.6e-12
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  +YHQETLENLK+A+KSTKKLCAV
Sbjct: 54 MSVARFDFSWGDAEYHQETLENLKVAIKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot
Match: B8BJ39 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.7e-12
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  +YHQETLENLK+A+KSTKKLCAV
Sbjct: 54 MSVARFDFSWGDAEYHQETLENLKLAIKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot
Match: Q2RAK2 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0148500 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.7e-12
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  +YHQETLENLK+A+KSTKKLCAV
Sbjct: 54 MSVARFDFSWGDAEYHQETLENLKLAIKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 5.9e-07
Identity = 24/36 (66.67%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          M+VARF+FS G+ +YHQETL+NLKIA+++T+ LCAV
Sbjct: 46 MNVARFNFSHGTHEYHQETLDNLKIAMQNTQILCAV 81

[Close]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL
Match: A0A5D3DH59 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00310 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-10
Identity = 40/54 (74.07%), Postives = 42/54 (77.78%), Query Frame = 0


Query: 1   MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAVC------SFLICLSVYFI 49
           MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV         L+ + V FI
Sbjct: 78  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAVMLDTAGPEVLVVIKVKFI 131

[Close]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL
Match: A0A5D3C7Q7 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00900 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-10
Identity = 35/36 (97.22%), Postives = 35/36 (97.22%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWGS DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWGSPDYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL
Match: A0A5A7SGM8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001520 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-10
Identity = 35/36 (97.22%), Postives = 35/36 (97.22%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWGS DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWGSPDYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL
Match: A0A1S3CH64 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103500862 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-10
Identity = 35/36 (97.22%), Postives = 35/36 (97.22%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWGS DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWGSPDYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL
Match: A0A0A0KKD4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G449820 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-10
Identity = 34/36 (94.44%), Postives = 35/36 (97.22%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWGS DYHQETLENLKIAVKSTKKLCA+
Sbjct: 54 MSVARFDFSWGSPDYHQETLENLKIAVKSTKKLCAI 89

[Close]

BLAST of IVF0018496.2 vs. NCBI nr
Match: KAA0033647.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa] >TYK22946.1 pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 75.9 bits (185), Expect = 1.80e-14
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 0


Query: 1   MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 36
           MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV
Sbjct: 78  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 113

[Close]

BLAST of IVF0018496.2 vs. NCBI nr
Match: XP_008467042.1 (PREDICTED: pyruvate kinase 2, cytosolic-like [Cucumis melo])

HSP 1 Score: 70.9 bits (172), Expect = 2.05e-14
Identity = 34/36 (94.44%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 36
          MSVARFD SWGS DYHQETLENLKIAVKSTKKLCAV
Sbjct: 26 MSVARFDISWGSPDYHQETLENLKIAVKSTKKLCAV 61

[Close]

BLAST of IVF0018496.2 vs. NCBI nr
Match: RWW55022.1 (hypothetical protein BHE74_00038366 [Ensete ventricosum] >RZS11538.1 hypothetical protein BHM03_00042860 [Ensete ventricosum])

HSP 1 Score: 70.9 bits (172), Expect = 1.12e-13
Identity = 32/36 (88.89%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 36
          MSVARFDFSWG +DYHQETLENLK A+KSTKKLCAV
Sbjct: 54 MSVARFDFSWGDVDYHQETLENLKAAIKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. NCBI nr
Match: XP_010497281.2 (PREDICTED: pyruvate kinase 2, cytosolic-like, partial [Camelina sativa])

HSP 1 Score: 68.9 bits (167), Expect = 1.21e-13
Identity = 33/36 (91.67%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 36
          MSVARFDFSW   DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. NCBI nr
Match: KAA0025298.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 73.2 bits (178), Expect = 2.10e-13
Identity = 35/36 (97.22%), Postives = 35/36 (97.22%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 36
          MSVARFDFSWGS DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWGSPDYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. TAIR 10
Match: AT3G52990.1 (Pyruvate kinase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-13
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  DYHQETL+NLK+AV+STKKLCAV
Sbjct: 54 MSVARFDFSWGDADYHQETLDNLKVAVRSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. TAIR 10
Match: AT3G52990.2 (Pyruvate kinase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-13
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSWG  DYHQETL+NLK+AV+STKKLCAV
Sbjct: 1  MSVARFDFSWGDADYHQETLDNLKVAVRSTKKLCAV 36

[Close]

BLAST of IVF0018496.2 vs. TAIR 10
Match: AT2G36580.1 (Pyruvate kinase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 5.6e-13
Identity = 33/36 (91.67%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARFDFSW   DYHQETLENLKIAVKSTKKLCAV
Sbjct: 54 MSVARFDFSWCDADYHQETLENLKIAVKSTKKLCAV 89

[Close]

BLAST of IVF0018496.2 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 2.1e-07
Identity = 24/36 (66.67%), Postives = 30/36 (83.33%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          MSVARF+FS GS +YHQETL+NL+ A+ +T  LCAV
Sbjct: 33 MSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLCAV 68

[Close]

BLAST of IVF0018496.2 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.0e-06
Identity = 23/36 (63.89%), Postives = 29/36 (80.56%), Query Frame = 0


Query: 1  MSVARFDFSWGSLDYHQETLENLKIAVKSTKKLCAV 37
          M+VARF+FS GS  YHQETL+NL+ A+ +T  LCAV
Sbjct: 42 MNVARFNFSHGSHSYHQETLDNLRTAMDNTGILCAV 77

The following BLAST results are available for this feature:

BLAST of IVF0018496.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8BM17	3.6e-12	86.11	Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456...	[more]
Q2QXR8	3.6e-12	86.11	Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g01...	[more]
B8BJ39	4.7e-12	86.11	Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105...	[more]
Q2RAK2	4.7e-12	86.11	Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g01...	[more]
P22200	5.9e-07	66.67	Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of IVF0018496.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DH59	1.2e-10	74.07	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G0031...	[more]
A0A5D3C7Q7	1.6e-10	97.22	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G0090...	[more]
A0A5A7SGM8	1.6e-10	97.22	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G0015...	[more]
A0A1S3CH64	1.6e-10	97.22	Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103500862 PE=3 SV=1	[more]
A0A0A0KKD4	2.0e-10	94.44	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G449820 PE=3 SV=1	[more]

BLAST of IVF0018496.2 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0033647.1	1.80e-14	100.00	pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa] >TYK22946.1 pyruvat...	[more]
XP_008467042.1	2.05e-14	94.44	PREDICTED: pyruvate kinase 2, cytosolic-like [Cucumis melo]	[more]
RWW55022.1	1.12e-13	88.89	hypothetical protein BHE74_00038366 [Ensete ventricosum] >RZS11538.1 hypothetica...	[more]
XP_010497281.2	1.21e-13	91.67	PREDICTED: pyruvate kinase 2, cytosolic-like, partial [Camelina sativa]	[more]
KAA0025298.1	2.10e-13	97.22	pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa]	[more]

BLAST of IVF0018496.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52990.1	3.3e-13	86.11	Pyruvate kinase family protein	[more]
AT3G52990.2	3.3e-13	86.11	Pyruvate kinase family protein	[more]
AT2G36580.1	5.6e-13	91.67	Pyruvate kinase family protein	[more]
AT4G26390.1	2.1e-07	66.67	Pyruvate kinase family protein	[more]
AT3G25960.1	1.0e-06	63.89	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 1..37 e-value: 9.0E-6 score: 24.8
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 1..37 e-value: 7.1E-7 score: 30.4
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 1..37
None	No IPR available	PANTHER	PTHR11817:SF89	PYRUVATE KINASE	coord: 1..37
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 1..37

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
IVF0018496	IVF0018496	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0018496.2-exon	IVF0018496.2-exon-chr06:199888..200030	exon
IVF0018496.2-exon	IVF0018496.2-exon-chr06:200137..200143	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0018496.2-cds	IVF0018496.2-cds-chr06:199888..200030	CDS
IVF0018496.2-cds	IVF0018496.2-cds-chr06:200137..200143	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
IVF0018496.2	IVF0018496.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity