IVF0012952.1 (mRNA) Melon (IVF77) v1

Overview


Name	IVF0012952.1
Type	mRNA
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Retrovirus-related Pol polyprotein from transposon 17.6
Location	chr09: 12205191 .. 12205457 (-)
Sequence length	267
RNA-Seq Expression	IVF0012952.1
Synteny	IVF0012952.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGACGCAAGTGATGTGGTGGTAGGGGATATGCCGGGTCAAAAGAAGGATAAAGTGACTGACCCGATATACTATGCGATCAAAACTCTCAATGAAGCTGAAGAAAATTATACAACCATAGAGAAGGAGTTGCTTGCAATAGTCTTTGCGATAGAAAAGTTTAGGAGTTACATTGTTGGCTCCAAAGTAACGATACACTCTGATCATTCTGCGATCAGATATCTCATGGCGAAAAAGGACGCTAAGTCGTGGTTGATAAGATAG

mRNA sequence

ATGTGTGACGCAAGTGATGTGGTGGTAGGGGATATGCCGGGTCAAAAGAAGGATAAAGTGACTGACCCGATATACTATGCGATCAAAACTCTCAATGAAGCTGAAGAAAATTATACAACCATAGAGAAGGAGTTGCTTGCAATAGTCTTTGCGATAGAAAAGTTTAGGAGTTACATTGTTGGCTCCAAAGTAACGATACACTCTGATCATTCTGCGATCAGATATCTCATGGCGAAAAAGGACGCTAAGTCGTGGTTGATAAGATAG

Coding sequence (CDS)

ATGTGTGACGCAAGTGATGTGGTGGTAGGGGATATGCCGGGTCAAAAGAAGGATAAAGTGACTGACCCGATATACTATGCGATCAAAACTCTCAATGAAGCTGAAGAAAATTATACAACCATAGAGAAGGAGTTGCTTGCAATAGTCTTTGCGATAGAAAAGTTTAGGAGTTACATTGTTGGCTCCAAAGTAACGATACACTCTGATCATTCTGCGATCAGATATCTCATGGCGAAAAAGGACGCTAAGTCGTGGTTGATAAGATAG

Protein sequence

MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGSKVTIHSDHSAIRYLMAKKDAKSWLIR

Homology

[Close]

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-11
Identity = 41/86 (47.67%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 3   DASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGS 62
           DASDV +G +  Q       P+ Y  +TLNE E NY+TIEKELLAIV+A + FR Y++G 
Sbjct: 514 DASDVALGAVLSQD----GHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGR 573

Query: 63  KVTIHSDHSAIRYLMAKKDAKSWLIR 89
              I SDH  + +L   KD  S L R
Sbjct: 574 HFEISSDHQPLSWLYRMKDPNSKLTR 595

[Close]

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 8.6e-09
Identity = 36/86 (41.86%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 3   DASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGS 62
           DAS++ +G +  Q       PI +  +TLN+ E NY+ IEKELLAIV+A + FR Y++G 
Sbjct: 513 DASNLALGAVLSQN----GHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGR 572

Query: 63  KVTIHSDHSAIRYLMAKKDAKSWLIR 89
           +  I SDH  +R+L   K+  + L R
Sbjct: 573 QFLIASDHQPLRWLHNLKEPGAKLER 594

[Close]

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.5e-08
Identity = 29/87 (33.33%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 3   DASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGS 62
           DAS+  +G +  Q       PI Y  ++LN+ EENY TIEKE+LAI+++++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

Query: 63  -KVTIHSDHSAIRYLMAKKDAKSWLIR 89
             + +++DH  + + +  ++  + L R
Sbjct: 500 GTIKVYTDHQPLTFALGNRNFNAKLKR 526

[Close]

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.2e-06
Identity = 31/86 (36.05%), Postives = 44/86 (51.16%), Query Frame = 0


Query: 3   DASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGS 62
           DAS    G +  Q  +    P+ YA +   + E N +T E+EL AI +AI  FR YI G 
Sbjct: 621 DASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGK 680

Query: 63  KVTIHSDHSAIRYLMAKKDAKSWLIR 89
             T+ +DH  + YL +  +  S L R
Sbjct: 681 HFTVKTDHRPLTYLFSMVNPSSKLTR 706

[Close]

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot
Match: P10401 (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.0e-05
Identity = 24/67 (35.82%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 23  PIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIVGSK-VTIHSDHSAIRYLMAKKD 82
           PI    +TL + E+NY T E+ELLAIV+A+ K ++++ GS+ + I +DH  + + +A ++
Sbjct: 516 PITMISRTLKQPEQNYATNERELLAIVWALGKLQNFLYGSREINIFTDHQPLTFAVADRN 575

Query: 83  AKSWLIR 89
             + + R
Sbjct: 576 TNAKIKR 582

[Close]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL
Match: A0A5D3DS34 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001700 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-41
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 89
           GSKVTIHSDHSAIRYLMAKKDAKSWLIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL
Match: A0A5A7V586 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002210 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-41
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 89
           GSKVTIHSDHSAIRYLMAKKDAKSWLIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL
Match: A0A5A7V8R9 (RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G003510 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-27
Identity = 68/87 (78.16%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVV+G M GQK DKV  PIY   KTLNEA+ENYTT EKELLA+VFAIEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLI 88
           GSKVTIHSDH AIRYLM KKDA+  LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

[Close]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL
Match: A0A2G9HH15 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 6.2e-26
Identity = 59/88 (67.05%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1    MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
            MCDASD  +G + GQ+KDK+   IYYA KTLN+A+ NYTT EKELLA+VFA +KFRSY+V
Sbjct: 1195 MCDASDFAIGAVLGQRKDKIFRSIYYASKTLNDAQLNYTTTEKELLAVVFAFDKFRSYLV 1254

Query: 61   GSKVTIHSDHSAIRYLMAKKDAKSWLIR 89
            G+KV +++DH+AIRYL+ KKDAK WLIR
Sbjct: 1255 GTKVIVYTDHAAIRYLIEKKDAKPWLIR 1282

[Close]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL
Match: A0A2G9H400 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_15163 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.1e-25
Identity = 58/88 (65.91%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASD  +G + GQ+KDK+   IYYA KTLN+A+ NYTT EKELLA+VFA +KFRSY+V
Sbjct: 880 MCDASDFAIGVVLGQRKDKIFRSIYYASKTLNDAQLNYTTTEKELLAVVFAFDKFRSYLV 939

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 89
           G+KV +++DH+AIRYL+ KKDA  WLIR
Sbjct: 940 GTKVIVYTDHAAIRYLIEKKDANPWLIR 967

[Close]

BLAST of IVF0012952.1 vs. NCBI nr
Match: KAA0062414.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (446), Expect = 8.65e-55
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 88
           GSKVTIHSDHSAIRYLMAKKDAKSWLIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of IVF0012952.1 vs. NCBI nr
Match: TYK26567.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (446), Expect = 1.98e-54
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 88
           GSKVTIHSDHSAIRYLMAKKDAKSWLIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of IVF0012952.1 vs. NCBI nr
Match: KAA0062295.1 (hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK26691.1 hypothetical protein E5676_scaffold313G003510 [Cucumis melo var. makuwa])

HSP 1 Score: 131 bits (330), Expect = 2.06e-37
Identity = 68/87 (78.16%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASDVV+G M GQK DKV  PIY   KTLNEA+ENYTT EKELLA+VFAIEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLI 87
           GSKVTIHSDH AIRYLM KKDA+  LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

[Close]

BLAST of IVF0012952.1 vs. NCBI nr
Match: WP_217833163.1 (Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. PCC 7002])

HSP 1 Score: 127 bits (320), Expect = 5.56e-36
Identity = 62/88 (70.45%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 1  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
          MCDAS+ VVG + GQ+K+K+  PIYYA KTLN  +ENYTT EKE+LAIVFA++KFR+Y +
Sbjct: 3  MCDASNHVVGAVLGQRKEKIMHPIYYASKTLNSFQENYTTTEKEMLAIVFAVDKFRAYFI 62

Query: 61 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 88
          GSKVTI+SDHSAI+YLM KKDAK  LIR
Sbjct: 63 GSKVTIYSDHSAIKYLMVKKDAKPRLIR 90

[Close]

BLAST of IVF0012952.1 vs. NCBI nr
Match: MCI26715.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 122 bits (305), Expect = 7.70e-33
Identity = 60/88 (68.18%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 1   MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 60
           MCDASD VVG + GQ+ DK    IYYA +TLNEA+ NYTT EKELLA+VFA +KFRSY+V
Sbjct: 91  MCDASDFVVGAVLGQRHDKTFHSIYYASRTLNEAQLNYTTTEKELLAVVFAFDKFRSYLV 150

Query: 61  GSKVTIHSDHSAIRYLMAKKDAKSWLIR 88
           G+KV +++DHSAI+YL+AKKDAK  LIR
Sbjct: 151 GTKVVVYTDHSAIKYLIAKKDAKPRLIR 178

The following BLAST results are available for this feature:

BLAST of IVF0012952.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	1.4e-11	47.67	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	8.6e-09	41.86	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
Q8I7P9	1.5e-08	33.33	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P10394	5.2e-06	36.05	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
P10401	2.0e-05	35.82	Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogas...	[more]

BLAST of IVF0012952.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DS34	5.2e-41	100.00	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V586	5.2e-41	100.00	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V8R9	1.5e-27	78.16	RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A2G9HH15	6.2e-26	67.05	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10756 PE=...	[more]
A0A2G9H400	3.1e-25	65.91	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_15163 PE=...	[more]

BLAST of IVF0012952.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0062414.1	8.65e-55	100.00	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
TYK26567.1	1.98e-54	100.00	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
KAA0062295.1	2.06e-37	78.16	hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK2669...	[more]
WP_217833163.1	5.56e-36	70.45	Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. ...	[more]
MCI26715.1	7.70e-33	68.18	hypothetical protein [Trifolium medium]	[more]

BLAST of IVF0012952.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 2..86 e-value: 2.2E-24 score: 85.8
None	No IPR available	GENE3D	3.10.20.370		coord: 1..68 e-value: 4.3E-6 score: 28.6
None	No IPR available	CDD	cd09274	RNase_HI_RT_Ty3	coord: 1..88 e-value: 2.39747E-34 score: 111.816
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..87

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
IVF0012952	IVF0012952	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0012952.1-exon	IVF0012952.1-exon-chr09:12205191..12205457	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0012952.1-cds	IVF0012952.1-cds-chr09:12205191..12205457	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
IVF0012952.1	IVF0012952.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003676	nucleic acid binding