IVF0008535.2 (mRNA) Melon (IVF77) v1

Overview
NameIVF0008535.2
TypemRNA
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationchr02: 1018971 .. 1021968 (+)
Sequence length1152
RNA-Seq ExpressionIVF0008535.2
SyntenyIVF0008535.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGTCTATATAGTTCTACCTTAATTAAAATATGATTTTAGAATTGGAAGCAAAAATCTATCTTAAAACGGCGAGTACCCGCGCTTCCATTTCACTTCATTTTGTCATCAACTCTTCAATTTCTTTCCAACTTCTTCTCTAGTTTCATATCGATTATAATTTCTCGAGGCGATTTTTTCCAGCATATCTGGAAGCCAATATAGTTGTAATTCATCAATTTGAGGTGACCCAATTTTTTTCTCCAAATTTGGAGTGAGTGTTTGTTCATAAAGCATAATTTTGTTTATGGGTTTCTTCACTAATCTTTGTATATGGCAGTTTTATTGTTCTCTGTTATTCCTTACTTCAATTTGTTCGCTGTTCTGTTGAAATTATCAGTGCATTTACACGTTGAAACTTGAGATTAACTTAAATTTAGAATATGGGTTCGTGTTTATGGCTGTTGTTGTATTTCTACATTGTTGTCATGGAATTAATAATTTGGAAGTTTCTTACATGAATTGTATTGTTATGATTTTTTTTTTTTATTTACTGAGCGGTTTCTGCTGCTGTGTTCCGTAGTTATGGAGATAAAGACGGATAGTAATGATGGCACGCTCATTTTAGTTAAGCAAGGAGCTGAAGCTGTGAGTTATAATCTCTTGTTGTTCTTTGGTCTGGGATGTTTTTTGGAATTGGGATGGTTATGGATAATGGAATATTTTGCTTTGATGTTCAATGAGTATGAGATGATTTTTAAAAGAGATTAGCGTGTATTCTCTTTGAAAACTTTTGAAATAAGCACTAATTAAGCGGTTTGGAATTTTCTAAATACTTTTCACTAGCCAAATTTGAGGAGTTTAAAAAGTGCTTCTGTTTTTCAAAAGTCATATCGAACTCATCCCTGGAATTTTAGTTGGACAAGTCTTTTGATAGTATTTTTTGTGGTTTTCGTAAATTAGTTTTCTTTTGTTCTCATCTTGTAGAGAGTGTTCGAGTCTACTTTCGTTGGAAGGAGATCGATTGTTAAGGAACGGTTCTCAAAGAAATATAGACATCCATTACTGGATTCCAAGCTTACACTCAAGCGATTGAATGCAGTTTGTTTCTTCTAATCAAACACTCTTTAGTATTAAAGAAATAACTAGCCAAAAGATTTTCTTGCATATATTTGACTGGAACTTCATGTTAGGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTAGCTACACCAGTGTTGTATGCTGTTGACCCGATATTATATACCCTGACATTTGAGTATGTGGAAGGTTCTTCTGTGAAAGATATATTGCTTGAAATTGGGTCAAATGGTGGTGATTCAAAACGGTTGAGTGACATTGCAATGCAGATTGGTGTTGCAATTGGGAAGTTACATGATGGTGGTTTAATTCATGGTGACCTGACCACATCAAACATGTTGATCAGGAGTGGTACCAATGAACTTGTGAGTTACATATTTAGTTATTATAATGTTCAAAGAGAGTTGTTTTAATTAATCTCCGTCATAAGGGCAACTGTCTTTTCAGGTTCTTATTGATTTTGGTCTGAGCTTCACTTCAACTATTCCAGAAGACAAAGCTGTTGATCTATATGTGTTGGAACGAGCGCTGCTATCATTGCATTCTTCATGTGGGAATTTGGTAAGTTGGTTTCCCTTGGCCTCGTTGTAAACAAAATTTGTTGTCTCCTCCATGGATAAGGGCAGTATCCTCTAGAGTGCTGGTTATTGTGTTGTGTTATGGGGATATATATGGCTTGAGAGAAGTAATAAAATCTTGGGGATGGGGAAATGGAAAGACATTGAGAGGAGGTCTAGTCCCATGCTATGTCTGAAACCTCACTTTTGGCTTTTATAATTACTTGTTAGGCTTGGCCTTGCTTGTTTTGGACGGGAGTCTTTTTCTTTATTTTTTTTATTTTGAGTTAGATTTAGATCTCCTTTGGGCTGTTATTTTGTAGAGCCACTCGTTGGCTTTCTTGTTTTCCTAATGAGTAATGAAAGCCTGATTTCTCGGTAAAAGGACATGGGAAAAACACTAATATATAAACTTTCCCTCTTAAATCATACGACTTAAGTGGTCTTTGAAAATGCTCATCATAGTCATATGAAGAAACACATGAGGAATGAGGGTCGATGAGGTAATGCATTTAGTTAGGAAGAAACATTGCAATGTGTGGTAACTGCTTATTGTTTTCGCCTCTAATTGGACATTTATGAGCCGTTTTTTGTGCTCAAATTCAGTCGGGTGGAGTGAGCAGGGATTGAGCCTCCGATTATAATAGTTGGTGTGTTGAACTACAATAACATACGATTAACTACAGCAATACCATTTGAAATCCCTCTGTTTTATTGTTTACTAAAATCTAACCATCTTCAAACTATAACAACCATAGACTACATAATATCCAACTCACTGCTTCAAATGCCTCCTATATATGTTTTGTCCTCGAAATCTTATGACTACTTATTGGCAAATCGAGTTGAATTGAAACTGAAATTGAATATGCTTTGAATGGTTCTACATTTTCATAGATGGAGTTAATTCTTGCTTCATACCGGAAGACTTCGAAGCAATGGTCGTCAACCTCGAACAAATTGGCTCAAGGTATTGATTCTGTCCTTTTCGTGTATTTTGTTTCTACTTATATATCAATTTCGTTCCATATGATTCATTGCTTCTTTTGTTTTGACTTTGATTTGTGTACAGTGCGTCAGAGAGGCCGAAAACGCACCATGGTTGGTTAAGTAATTTCACTTTTAATCTGTCTTGTTGAATTATCACTTGACTTCTTGTGTAGTAGACTCATTTGAACTTCAACAACGTATTTAATTAATCTTAATCTTAAAAACAAATCAACAATTTTGTTTTAGTGATTGCAATTTTGTGGGAAGAGTAGAGTTTTTTTTTGGTACAATGTAAAAAAGTATAAAAAATGAAAAGAACAAATTACTGAGTTCAGTAGTGTTTATTATTGAAGTTGAGTTATCGAG

mRNA sequence

TCTGTCTATATAGTTCTACCTTAATTAAAATATGATTTTAGAATTGGAAGCAAAAATCTATCTTAAAACGGCGAGTACCCGCGCTTCCATTTCACTTCATTTTGTCATCAACTCTTCAATTTCTTTCCAACTTCTTCTCTAGTTTCATATCGATTATAATTTCTCGAGGCGATTTTTTCCAGCATATCTGGAAGCCAATATAGTTGTAATTCATCAATTTGAGTTATGGAGATAAAGACGGATAGTAATGATGGCACGCTCATTTTAGTTAAGCAAGGAGCTGAAGCTAGAGTGTTCGAGTCTACTTTCGTTGGAAGGAGATCGATTGTTAAGGAACGGTTCTCAAAGAAATATAGACATCCATTACTGGATTCCAAGCTTACACTCAAGCGATTGAATGCAGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTAGCTACACCAGTGTTGTATGCTGTTGACCCGATATTATATACCCTGACATTTGAGTATGTGGAAGGTTCTTCTGTGAAAGATATATTGCTTGAAATTGGGTCAAATGGTGGTGATTCAAAACGGTTGAGTGACATTGCAATGCAGATTGGTGTTGCAATTGGGAAGTTACATGATGGTGGTTTAATTCATGGTGACCTGACCACATCAAACATGTTGATCAGGAGTGGTACCAATGAACTTGTTCTTATTGATTTTGGTCTGAGCTTCACTTCAACTATTCCAGAAGACAAAGCTGTTGATCTATATGTGTTGGAACGAGCGCTGCTATCATTGCATTCTTCATGTGGGAATTTGATGGAGTTAATTCTTGCTTCATACCGGAAGACTTCGAAGCAATGGTCGTCAACCTCGAACAAATTGGCTCAAGTGCGTCAGAGAGGCCGAAAACGCACCATGGTTGGTTAAGTAATTTCACTTTTAATCTGTCTTGTTGAATTATCACTTGACTTCTTGTGTAGTAGACTCATTTGAACTTCAACAACGTATTTAATTAATCTTAATCTTAAAAACAAATCAACAATTTTGTTTTAGTGATTGCAATTTTGTGGGAAGAGTAGAGTTTTTTTTTGGTACAATGTAAAAAAGTATAAAAAATGAAAAGAACAAATTACTGAGTTCAGTAGTGTTTATTATTGAAGTTGAGTTATCGAG

Coding sequence (CDS)

ATGGAGATAAAGACGGATAGTAATGATGGCACGCTCATTTTAGTTAAGCAAGGAGCTGAAGCTAGAGTGTTCGAGTCTACTTTCGTTGGAAGGAGATCGATTGTTAAGGAACGGTTCTCAAAGAAATATAGACATCCATTACTGGATTCCAAGCTTACACTCAAGCGATTGAATGCAGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTAGCTACACCAGTGTTGTATGCTGTTGACCCGATATTATATACCCTGACATTTGAGTATGTGGAAGGTTCTTCTGTGAAAGATATATTGCTTGAAATTGGGTCAAATGGTGGTGATTCAAAACGGTTGAGTGACATTGCAATGCAGATTGGTGTTGCAATTGGGAAGTTACATGATGGTGGTTTAATTCATGGTGACCTGACCACATCAAACATGTTGATCAGGAGTGGTACCAATGAACTTGTTCTTATTGATTTTGGTCTGAGCTTCACTTCAACTATTCCAGAAGACAAAGCTGTTGATCTATATGTGTTGGAACGAGCGCTGCTATCATTGCATTCTTCATGTGGGAATTTGATGGAGTTAATTCTTGCTTCATACCGGAAGACTTCGAAGCAATGGTCGTCAACCTCGAACAAATTGGCTCAAGTGCGTCAGAGAGGCCGAAAACGCACCATGGTTGGTTAA

Protein sequence

MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Homology
BLAST of IVF0008535.2 vs. ExPASy Swiss-Prot
Match: Q54W07 (EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-47
Identity = 102/214 (47.66%), Postives = 137/214 (64.02%), Query Frame = 0

Query: 13  ILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLGV 72
           IL+ QGAEA+ +E+   G + IVKERFSK YRHP+LD K++ KR+  E R + K ++ G+
Sbjct: 39  ILISQGAEAKTYETDLYGLKCIVKERFSKAYRHPILDQKISSKRILQEVRSLNKCKKKGI 98

Query: 73  ATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHDGG 132
             P LY VD     +  E+++G +VK  L +   +     ++  I  ++G  IG +H+  
Sbjct: 99  QVPSLYLVDIGNNRIYMEFIKGETVKHYLYKNQESTQHQNQIESIMKELGNQIGIIHEMN 158

Query: 133 LIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSLHSSCGNLME 192
           +IHGDLTTSNML+R  TNELV IDFGLS+TS   EDKAVDLYVLERA +S H +   L +
Sbjct: 159 VIHGDLTTSNMLLRESTNELVFIDFGLSYTSNSVEDKAVDLYVLERAFISTHPNSEQLFQ 218

Query: 193 LILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
            IL++Y  TS        KL QVR RGRK+T  G
Sbjct: 219 TILSNYELTSSNSKIVIQKLNQVRLRGRKKTCFG 252

BLAST of IVF0008535.2 vs. ExPASy Swiss-Prot
Match: Q96S44 (EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 4.6e-46
Identity = 106/218 (48.62%), Postives = 140/218 (64.22%), Query Frame = 0

Query: 12  LILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLG 71
           L LVKQGAEARVF   F GR +++K RF K YRHP L+++L  +R   EAR + + RR G
Sbjct: 36  LELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAG 95

Query: 72  VATPVLYAVDPILYTLTFEYVEGS-SVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHD 131
           ++ PV++ VD     L  E +EGS +V+D +          + LS++A  IG  + ++HD
Sbjct: 96  ISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQVLARMHD 155

Query: 132 GGLIHGDLTTSNMLIRSGTNEL--VLIDFGLSFTSTIPEDKAVDLYVLERALLSLHSSCG 191
             LIHGDLTTSNML++    +L  VLIDFGLSF S +PEDK VDLYVLE+A LS H +  
Sbjct: 156 EDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTE 215

Query: 192 NLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
            + E  L SY  +SK+      KL +VR RGRKR+MVG
Sbjct: 216 TVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG 253

BLAST of IVF0008535.2 vs. ExPASy Swiss-Prot
Match: Q99PW4 (EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.0e-45
Identity = 108/218 (49.54%), Postives = 138/218 (63.30%), Query Frame = 0

Query: 12  LILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLG 71
           L LV+QGAEARVF   F GR ++VK RF K YRHP L+++L  +R   EAR + + RR G
Sbjct: 27  LELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAG 86

Query: 72  VATPVLYAVDPILYTLTFEYVEGS-SVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHD 131
           +A PV++ VD     L  E +E S +V+D +        D + L D+A ++G  +  +HD
Sbjct: 87  IAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQSTMETEKDPQCLLDLARRMGQVLAGMHD 146

Query: 132 GGLIHGDLTTSNMLIRSGTNEL--VLIDFGLSFTSTIPEDKAVDLYVLERALLSLHSSCG 191
             LIHGDLTTSNML+R    +L  VLIDFGLSF S +PEDK VDLYVLE+A LS H    
Sbjct: 147 QDLIHGDLTTSNMLLRRPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTE 206

Query: 192 NLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
              E  L SY  +SK+ S    KL +VR RGRKR+MVG
Sbjct: 207 TAFEAFLKSYGASSKKSSPVLKKLDEVRLRGRKRSMVG 244

BLAST of IVF0008535.2 vs. ExPASy Swiss-Prot
Match: Q9P7N1 (EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP27G11.07c PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 6.6e-37
Identity = 92/227 (40.53%), Postives = 136/227 (59.91%), Query Frame = 0

Query: 2   EIKTDSNDGTLILVKQGAEARVFESTFV-GRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 61
           +I  +  +  L +VKQGAEA   ++ F  G   ++K R +K++RHP+LD KL+ KR   E
Sbjct: 13  DIYREIKEKKLTVVKQGAEAITIKTEFYPGEVCLLKCRPAKRWRHPILDQKLSRKRCLVE 72

Query: 62  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 121
           AR + K   +G+  P+LY +D     +  E+++G  V+D + EI       K+L  +  +
Sbjct: 73  ARLLAKCHYVGIKCPMLYFIDANRGQIYMEWIDGPCVRDYIREI-CECEIEKKLIPLMKR 132

Query: 122 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNEL-VLIDFGLSFTSTIPEDKAVDLYVLERA 181
           IG  + K+H   ++HGDLTTSNM++ S  N + + IDFGL   S   EDKAVD+YVLERA
Sbjct: 133 IGSEVAKMHKNDIVHGDLTTSNMMLESHNNPVPIFIDFGLGSVSESEEDKAVDIYVLERA 192

Query: 182 LLSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           L S      +L   +L SY ++ KQ  +T  +  +VR RGRKRTM+G
Sbjct: 193 LSSTLPESESLFHHVLDSYAQSWKQSKATLRRFEEVRMRGRKRTMIG 238

BLAST of IVF0008535.2 vs. ExPASy Swiss-Prot
Match: Q2HGY8 (EKC/KEOPS complex subunit BUD32 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=BUD32 PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 8.7e-37
Identity = 99/243 (40.74%), Postives = 138/243 (56.79%), Query Frame = 0

Query: 14  LVKQGAEARVFESTFV--GRRSIVKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLG 73
           L+ QGAE R++++T +   R   +K R  K YRHP+LD++LT  RL++EA+ + +  R G
Sbjct: 25  LITQGAEGRLYKTTHLTRDRPCALKYRPPKPYRHPVLDARLTKARLSSEAKVLERCWREG 84

Query: 74  VATPVLYAVDPILYTLTFEYVEGSSVKDILLE-IGSNG---------GDSKRLSDIAMQI 133
           V  P +YA+DP    +  E++EG  V+  + E +G             D   + D+  +I
Sbjct: 85  VPVPAVYAMDPAAGWMMMEWIEGIPVRVGINEWLGDRPEEGAEIPQVADETPIVDLMKRI 144

Query: 134 GVAIGKLHDGGLIHGDLTTSNMLIR------------------SGTNELVLIDFGLSFTS 193
           G AIG LH  G++HGDLTTSNM++R                  S   ++VLIDFGL+  S
Sbjct: 145 GAAIGALHKTGVVHGDLTTSNMMLRPRGFNPVDGAPGDEGKAGSVEGDVVLIDFGLATQS 204

Query: 194 TIPEDKAVDLYVLERALLSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRT 227
              ED+AVDLYVLERA  S H     L   +L SY+ T K+ SS   KL  VR RGRKR+
Sbjct: 205 MSDEDRAVDLYVLERAFASTHPRAERLFATLLESYKSTFKKASSVLIKLEDVRMRGRKRS 264

BLAST of IVF0008535.2 vs. ExPASy TrEMBL
Match: A0A1S3BPU1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103492219 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.0e-117
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ
Sbjct: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. ExPASy TrEMBL
Match: A0A5D3CF78 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003400 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.0e-117
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ
Sbjct: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. ExPASy TrEMBL
Match: A0A0A0LW55 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G573000 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.2e-113
Identity = 216/224 (96.43%), Postives = 223/224 (99.55%), Query Frame = 0

Query: 3   IKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEAR 62
           ++TDSNDGTLILVKQGAEARVFESTFVGRRSI+KERFSKKYRHPLLDSKLTLKRLNAEAR
Sbjct: 1   METDSNDGTLILVKQGAEARVFESTFVGRRSIIKERFSKKYRHPLLDSKLTLKRLNAEAR 60

Query: 63  CMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIG 122
           CMTKARRLGVATPVLYAVDPILYTLTFEYVEGS VKDILLEIGS+GGDSK+LSDIAMQIG
Sbjct: 61  CMTKARRLGVATPVLYAVDPILYTLTFEYVEGSPVKDILLEIGSSGGDSKQLSDIAMQIG 120

Query: 123 VAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS 182
           VAIGKLHDGGL+HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS
Sbjct: 121 VAIGKLHDGGLVHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS 180

Query: 183 LHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           +HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 MHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 224

BLAST of IVF0008535.2 vs. ExPASy TrEMBL
Match: A0A6J1DK25 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111021824 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.0e-109
Identity = 209/226 (92.48%), Postives = 220/226 (97.35%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDG+LIL+KQGAEARVFESTFVGRRSI+KERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGV+TPVLYAVDP L+TLTFEYVEG +VKDILLEIGS+G  +K+L+DIAMQ
Sbjct: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LS+HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. ExPASy TrEMBL
Match: A0A6J1I5M0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111470128 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 6.7e-109
Identity = 208/226 (92.04%), Postives = 218/226 (96.46%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDG+L+L+KQGAEARVFESTFVGRRSIVKERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRL V+TPVLYAVDPIL+TLTFEYVEG SVKDILLEIGS+G  SK+L DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LS+HSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. NCBI nr
Match: XP_008450724.1 (PREDICTED: EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis melo] >XP_008450725.1 PREDICTED: EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis melo] >TYK10195.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 432 bits (1111), Expect = 2.10e-152
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ
Sbjct: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. NCBI nr
Match: XP_011659926.1 (EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_011659927.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_011659928.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_031736098.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_031736107.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_031736109.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_031736111.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >KGN66130.1 hypothetical protein Csa_007401 [Cucumis sativus])

HSP 1 Score: 419 bits (1076), Expect = 4.23e-147
Identity = 216/224 (96.43%), Postives = 223/224 (99.55%), Query Frame = 0

Query: 3   IKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAEAR 62
           ++TDSNDGTLILVKQGAEARVFESTFVGRRSI+KERFSKKYRHPLLDSKLTLKRLNAEAR
Sbjct: 1   METDSNDGTLILVKQGAEARVFESTFVGRRSIIKERFSKKYRHPLLDSKLTLKRLNAEAR 60

Query: 63  CMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIG 122
           CMTKARRLGVATPVLYAVDPILYTLTFEYVEGS VKDILLEIGS+GGDSK+LSDIAMQIG
Sbjct: 61  CMTKARRLGVATPVLYAVDPILYTLTFEYVEGSPVKDILLEIGSSGGDSKQLSDIAMQIG 120

Query: 123 VAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS 182
           VAIGKLHDGGL+HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS
Sbjct: 121 VAIGKLHDGGLVHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLS 180

Query: 183 LHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           +HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 MHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 224

BLAST of IVF0008535.2 vs. NCBI nr
Match: XP_038880661.1 (EKC/KEOPS complex subunit bud32 isoform X1 [Benincasa hispida] >XP_038880662.1 EKC/KEOPS complex subunit bud32 isoform X1 [Benincasa hispida] >XP_038880663.1 EKC/KEOPS complex subunit bud32 isoform X1 [Benincasa hispida])

HSP 1 Score: 417 bits (1072), Expect = 1.85e-146
Identity = 216/226 (95.58%), Postives = 223/226 (98.67%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSND +LILVKQGAEARVFESTFVGRRSI+KERFSKKYRHPLLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDDSLILVKQGAEARVFESTFVGRRSIIKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGV+TPVLYAVDPILYTLTFEYVEG SVKDILLEIGSN GDSK+LSDIAMQ
Sbjct: 61  ARCMTKARRLGVSTPVLYAVDPILYTLTFEYVEGCSVKDILLEIGSNDGDSKQLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNMLIR+GTN+LVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRNGTNDLVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LS+HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. NCBI nr
Match: XP_022154600.1 (EKC/KEOPS complex subunit bud32 [Momordica charantia] >XP_022154601.1 EKC/KEOPS complex subunit bud32 [Momordica charantia])

HSP 1 Score: 405 bits (1042), Expect = 6.92e-142
Identity = 209/226 (92.48%), Postives = 220/226 (97.35%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDG+LIL+KQGAEARVFESTFVGRRSI+KERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRLGV+TPVLYAVDP L+TLTFEYVEG +VKDILLEIGS+G  +K+L+DIAMQ
Sbjct: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LS+HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. NCBI nr
Match: XP_023529406.1 (EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 405 bits (1040), Expect = 1.40e-141
Identity = 209/226 (92.48%), Postives = 218/226 (96.46%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           MEIKTDSNDG+L+L+KQGAEARVFESTFVGRRSIVKERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKARRL V+TPVLYAVDPILYTLTFEYVEG SVKDILLEIGS+G  SK+L DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLIHGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LS+HSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

BLAST of IVF0008535.2 vs. TAIR 10
Match: AT5G26110.1 (Protein kinase superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 2.6e-97
Identity = 176/226 (77.88%), Postives = 202/226 (89.38%), Query Frame = 0

Query: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
           M+ + +  D +L+L+KQGAEARVFESTF GRRSIVKERFSKKYRHP+LD+KLTLKRLNAE
Sbjct: 1   MDCEENVRDESLVLIKQGAEARVFESTFAGRRSIVKERFSKKYRHPILDAKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120
           ARCMTKAR+LGV TPVLYAVD +L++LT EY+EG SVKDI LE G+NG   +RL D+A Q
Sbjct: 61  ARCMTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDIFLEFGTNGVVEERLDDVAAQ 120

Query: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IG AI KLHDGGL HGDLTTSNML+RSGTN+LVLIDFGLS TST+PEDKAVDLYVLERAL
Sbjct: 121 IGAAIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLSVTSTLPEDKAVDLYVLERAL 180

Query: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LS+HSSCGN+M+ IL +YRK+SKQWS+T NKLAQVRQRGRKRTM+G
Sbjct: 181 LSMHSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGRKRTMIG 226

BLAST of IVF0008535.2 vs. TAIR 10
Match: AT5G26110.2 (Protein kinase superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 4.4e-68
Identity = 127/163 (77.91%), Postives = 145/163 (88.96%), Query Frame = 0

Query: 64  MTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIGV 123
           MTKAR+LGV TPVLYAVD +L++LT EY+EG SVKDI LE G+NG   +RL D+A QIG 
Sbjct: 1   MTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDIFLEFGTNGVVEERLDDVAAQIGA 60

Query: 124 AIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSL 183
           AI KLHDGGL HGDLTTSNML+RSGTN+LVLIDFGLS TST+PEDKAVDLYVLERALLS+
Sbjct: 61  AIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLSVTSTLPEDKAVDLYVLERALLSM 120

Query: 184 HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           HSSCGN+M+ IL +YRK+SKQWS+T NKLAQVRQRGRKRTM+G
Sbjct: 121 HSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGRKRTMIG 163

BLAST of IVF0008535.2 vs. TAIR 10
Match: AT1G08120.1 (BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (TAIR:AT5G26110.1); Has 235 Blast hits to 235 proteins in 96 species: Archae - 21; Bacteria - 0; Metazoa - 117; Fungi - 8; Plants - 47; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink). )

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-16
Identity = 45/70 (64.29%), Postives = 56/70 (80.00%), Query Frame = 0

Query: 1  MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60
          M+ + +  + +L+L+KQGAEARV ESTF GRRSIVKERFSKKYRHP+LD+KLTLKRL  +
Sbjct: 5  MDCEENVGNESLVLIKQGAEARVLESTFSGRRSIVKERFSKKYRHPILDAKLTLKRLYDK 64

Query: 61 ARCMTKARRL 71
           +   KAR L
Sbjct: 65 GK---KARGL 71

BLAST of IVF0008535.2 vs. TAIR 10
Match: AT5G01850.1 (Protein kinase superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 5.3e-05
Identity = 40/156 (25.64%), Postives = 70/156 (44.87%), Query Frame = 0

Query: 15  VKQGAEARVFESTFVGRRSI---VKERFSKKYRHPLLDSKLTLKRLNAEARCMTKARRLG 74
           + +GA  +V++  + GR+ +   V  R SK  +   L+S     R   E   M++ +   
Sbjct: 24  IGEGAHGKVYQGRY-GRQIVAIKVVNRGSKPDQQSSLES-----RFVREVNMMSRVQHHN 83

Query: 75  VATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHDG 134
           +   +    DP++  +T E + G S++  L  I             A+ I  A+  LH  
Sbjct: 84  LVKFIGACKDPLMVIVT-ELLPGMSLRKYLTSIRPQLLHLPLALSFALDIARALHCLHAN 143

Query: 135 GLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPE 168
           G+IH DL   N+L+      + L DFGL+   ++ E
Sbjct: 144 GIIHRDLKPDNLLLTENHKSVKLADFGLAREESVTE 172

BLAST of IVF0008535.2 vs. TAIR 10
Match: AT3G50720.1 (Protein kinase superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 2.6e-04
Identity = 27/86 (31.40%), Postives = 43/86 (50.00%), Query Frame = 0

Query: 80  VDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQIGVAIGKLHDGGLIHGDLT 139
           ++P L  +T E V G +++  +L    +  D K     A+ I  A+  LH  G+IH DL 
Sbjct: 117 IEPQLMIVT-ELVRGGTLQRFMLNSRPSPLDLKVSLSFALDISRAMEYLHSKGIIHRDLN 176

Query: 140 TSNMLIRSGTNELVLIDFGLSFTSTI 166
             N+L+      + L DFGL+   T+
Sbjct: 177 PRNVLVTGDMKHVKLADFGLAREKTL 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54W071.4e-4747.66EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE... [more]
Q96S444.6e-4648.62EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2[more]
Q99PW43.0e-4549.54EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1[more]
Q9P7N16.6e-3740.53EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972... [more]
Q2HGY88.7e-3740.74EKC/KEOPS complex subunit BUD32 OS=Chaetomium globosum (strain ATCC 6205 / CBS 1... [more]
Match NameE-valueIdentityDescription
A0A1S3BPU11.0e-117100.00Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5D3CF781.0e-117100.00Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0LW551.2e-11396.43Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A6J1DK251.0e-10992.48Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1I5M06.7e-10992.04Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_008450724.12.10e-152100.00PREDICTED: EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis melo] >XP_0084507... [more]
XP_011659926.14.23e-14796.43EKC/KEOPS complex subunit bud32 isoform X1 [Cucumis sativus] >XP_011659927.1 EKC... [more]
XP_038880661.11.85e-14695.58EKC/KEOPS complex subunit bud32 isoform X1 [Benincasa hispida] >XP_038880662.1 E... [more]
XP_022154600.16.92e-14292.48EKC/KEOPS complex subunit bud32 [Momordica charantia] >XP_022154601.1 EKC/KEOPS ... [more]
XP_023529406.11.40e-14192.48EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC... [more]
Match NameE-valueIdentityDescription
AT5G26110.12.6e-9777.88Protein kinase superfamily protein [more]
AT5G26110.24.4e-6877.91Protein kinase superfamily protein [more]
AT1G08120.11.3e-1664.29BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (... [more]
AT5G01850.15.3e-0525.64Protein kinase superfamily protein [more]
AT3G50720.12.6e-0431.40Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 9..225
e-value: 0.0024
score: -89.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 55..161
e-value: 1.9E-10
score: 40.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..226
score: 13.262652
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 110..223
e-value: 3.0E-34
score: 119.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 20..92
e-value: 4.9E-25
score: 88.7
NoneNo IPR availablePANTHERPTHR12209:SF1EKC/KEOPS COMPLEX SUBUNIT BUD32-LIKE ISOFORM X1coord: 1..226
IPR022495Serine/threonine-protein kinase Bud32TIGRFAMTIGR03724TIGR03724coord: 15..220
e-value: 3.4E-59
score: 197.6
IPR022495Serine/threonine-protein kinase Bud32PANTHERPTHR12209O-SIALOGLYCOPROTEIN ENDOPEPTIDASEcoord: 1..226
IPR008266Tyrosine-protein kinase, active sitePROSITEPS00109PROTEIN_KINASE_TYRcoord: 133..145
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 15..174

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
IVF0008535IVF0008535gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0008535.2-five_prime_utrIVF0008535.2-five_prime_utr-chr02:1018971..1019193five_prime_UTR
IVF0008535.2-five_prime_utrIVF0008535.2-five_prime_utr-chr02:1019532..1019533five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0008535.2-exonIVF0008535.2-exon-chr02:1018971..1019193exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1019532..1019596exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1019936..1020049exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1020142..1020420exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1020503..1020616exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1021510..1021582exon
IVF0008535.2-exonIVF0008535.2-exon-chr02:1021685..1021968exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1019534..1019596CDS
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1019936..1020049CDS
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1020142..1020420CDS
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1020503..1020616CDS
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1021510..1021582CDS
IVF0008535.2-cdsIVF0008535.2-cds-chr02:1021685..1021722CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0008535.2-three_prime_utrIVF0008535.2-three_prime_utr-chr02:1021723..1021968three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
IVF0008535.2IVF0008535.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0008033 tRNA processing
biological_process GO:0070525 tRNA threonylcarbamoyladenosine metabolic process
cellular_component GO:0000408 EKC/KEOPS complex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity