IVF0004590.2 (mRNA) Melon (IVF77) v1

Overview
NameIVF0004590.2
TypemRNA
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationchr02: 17245464 .. 17248690 (+)
Sequence length2398
RNA-Seq ExpressionIVF0004590.2
SyntenyIVF0004590.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGGGCCGTCATTTTCACTGCACAACTTTTTCTCTTCTCTCTTCTCTCTCAACTTTTTCTTCCGCAAAAATATGTGCTTTCCAAATACTTCTCTCCCCAAAAACTGAGGAGGAACCAAGGATTTGCTCTCCTCACCCACAACCCAAATCCCTTCATATCAAATGGACACAACCGATTCAATCACTGCTTTCTCTCATCTTCTTCAACCTCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGAGTACTTTTCTCTCTCTTTCTCTCTAACTATCTTCCCAATTCCCCATCGTTTTTATTCTCTTCTTTCCTGTTTTGCTTGCAGTATAATGAAATCCTCGGCAAAGGAGCTTCCAAGACAGTGTATGAACCAATTTAGCTTCCCCTTTGTTTTCCTTCCATTTTTGTAATTAATATCGAATTTTGGATTCCCTTGTAGTTTCAACCTTCTTTTTTTCTTTTTTGTCTCTATTTGATTCTCTTTCACATACATTTTCTATTTATTTTGAGAATTCGATCTCTTCTTGATGTTTGATTTTTGTTTATTTGGTTCTCTTTTTCGATTTCCGTTCATGGGTTATGTTTGATTTTGTTAATTTGTTTAGTTTTTGGAAATGAGCATTTCCCTGAGTTCTTTTTTTTTTTTTTTCACTTTATAGTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTCCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTAAAAACATTGAAACATAAAAACATAATGAAATTCTATACCTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTCTGAGGCAGTAAGAATGCTTTTCAACATTCGATCTTACACTCTTCAATTTCCATTCTCGTAATTGTTGCATTCTTTCTTTTAGATTTCTGCCTAATTTCTGGTTCGTTCTGGTTTCCAAGGTATAGGCTAAAGCATAGGAGAGCTAACATTAGGGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCATTATCTCCATAGCCAAGACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAGGGAGAAATTAAAATTGGAGACCTTGGTCTTGCTGCTATCCTCCAGAAATCACATGCTGATCATTGTGTTGGTATGTTTATCTTCTTCAAATCATGTGAAATTTTGGCAATAAATTCAGTTTTTGAAATGAAGGATCAGGTGGAGATTGTGTTACAGTTCTTTTAACATTTCATTTTTGGTTCTCTGATCCTGAATGCTCTTACGATGAATCACTTTCCTTGAGTTGGTGGTGATTTTTGCCAGAAATAAATCATTTTGTCAGATTAGTTTATTATAATTGTCATTACATTACAGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAGGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGTAAGATCTCCGTCTCTCAACAGGCCTATGCTTTTCTTTAATCTTCTATGTGAGAGAAGAAAAAGTTGATTACTTAACATGCTGTCTACTTGTAGGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCAAGCATGCGACAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTTGAAATTGATATTTGTGAAAGCAAGTTGAAAATTTCAGATTCTCGGAGAGAACTGGACGATTTTGCTTCCACTATAGTGCGGCCTTTTCTTGAACGAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTGGAGGCCTCAGATGAATGGAGGTACCGTTCAGTCCAGAAGGAACGTGATGGAATTGAACTTTTTGAGGACAATGATAATGACCAATTGGAAAATCTTGATAAAAATATTAAGGGGAAAATACGTGAAGATGGCAGCATTGTTTTAAGACTTAGGATTACGGATAAAGAAGGTAGCTCAGTACATTTATTTTCCTTTCGTTTTTGGTTATGGATGCTTGCTTTCTATTTATAGTTACAGAAATGTTCAACTGAACAATGCGGTATCTGTGCAGGGCTTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACAGCATTAACAGTAGCCACCGAAATGATTGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTTCTTCCTTGGTACCCGAGTGGAAGCCAGGGCCTGGCATAGACGAAACACCTCGCATCTCTTACGATGGTGGCTCTCAAAGCTACAACGCTTCTAATCAACCATCTGATAATATTTTGATAGAGAATAAAGGAAATGGAATCAAACTTTATCAGATTTTGAACTTAAGCACAGATGGGCATGCTTTGGCACATAAACATTTCGAGCAAGAGCAGTTCTCGCTCAAAGCAGATCGGCCTACACAACCTGTGGTAGGGAGCATACCAAATGTGTCAAGCCAGCACTATCAGCCAGATTCTGTCCTCCCTGAAAACCAAGCTCTTAGTTCTCGTAGTTTTAGGCAAAGACATTCTGATGATAATTACAAGAAAACTGACCAATCACTCACTGTTGGGTATAAAGAAAAACTACCAGATAAGAAAGCCACTGTCATTGACACTTCACAAAGAAGCTTATTGGGTTCCCGATCACTGTCCAGGGTTTCATCCTATTGCGAAGATAAATTTTCCTCTCAAATCCATTGGGAAATTAGATGGCTGTGGAATTGACAAAATTTAGATATTCGGTTTTTATTGGACAAGAGTGTGAAGATTGTAATCGTAGTTGGTTTGAGATTCATTTTGCTAGAGCTGCCCCGTTCGGATACCAAAAGGGTCCGAAGTTGCGAGGCGTTAGTGAGATTCTTTTATTGCAGAGTATATTTCAAGAATCAAAGCTTACAGGCACATTGCCTTCAAGCGACAGAACAACTCTCTACTCCACCAGTTGCCTGCTTTTTTGTTGTACAAAAATATGTAAGTTACTCACAGCACTCACTGAACTTTTTACACTTACAATGTCTTCTCTTCTCTTCT

mRNA sequence

AAAGGGGCCGTCATTTTCACTGCACAACTTTTTCTCTTCTCTCTTCTCTCTCAACTTTTTCTTCCGCAAAAATATGTGCTTTCCAAATACTTCTCTCCCCAAAAACTGAGGAGGAACCAAGGATTTGCTCTCCTCACCCACAACCCAAATCCCTTCATATCAAATGGACACAACCGATTCAATCACTGCTTTCTCTCATCTTCTTCAACCTCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGAGTACTTTTCTCTCTCTTTCTCTCTAACTATCTTCCCAATTCCCCATCGTTTTTATTCTCTTCTTTCCTGTTTTGCTTGCAGTATAATGAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTCCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTAAAAACATTGAAACATAAAAACATAATGAAATTCTATACCTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTCTGAGGCAGTATAGGCTAAAGCATAGGAGAGCTAACATTAGGGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCATTATCTCCATAGCCAAGACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAGGGAGAAATTAAAATTGGAGACCTTGGTCTTGCTGCTATCCTCCAGAAATCACATGCTGATCATTGTGTTGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAGGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCAAGCATGCGACAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTTGAAATTGATATTTGTGAAAGCAAGTTGAAAATTTCAGATTCTCGGAGAGAACTGGACGATTTTGCTTCCACTATAGTGCGGCCTTTTCTTGAACGAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTGGAGGCCTCAGATGAATGGAGGTACCGTTCAGTCCAGAAGGAACGTGATGGAATTGAACTTTTTGAGGACAATGATAATGACCAATTGGAAAATCTTGATAAAAATATTAAGGGGAAAATACGTGAAGATGGCAGCATTGTTTTAAGACTTAGGATTACGGATAAAGAAGGGCTTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACAGCATTAACAGTAGCCACCGAAATGATTGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTTCTTCCTTGGTACCCGAGTGGAAGCCAGGGCCTGGCATAGACGAAACACCTCGCATCTCTTACGATGGTGGCTCTCAAAGCTACAACGCTTCTAATCAACCATCTGATAATATTTTGATAGAGAATAAAGGAAATGGAATCAAACTTTATCAGATTTTGAACTTAAGCACAGATGGGCATGCTTTGGCACATAAACATTTCGAGCAAGAGCAGTTCTCGCTCAAAGCAGATCGGCCTACACAACCTGTGGTAGGGAGCATACCAAATGTGTCAAGCCAGCACTATCAGCCAGATTCTGTCCTCCCTGAAAACCAAGCTCTTAGTTCTCGTAGTTTTAGGCAAAGACATTCTGATGATAATTACAAGAAAACTGACCAATCACTCACTGTTGGGTATAAAGAAAAACTACCAGATAAGAAAGCCACTGTCATTGACACTTCACAAAGAAGCTTATTGGGTTCCCGATCACTGTCCAGGGTTTCATCCTATTGCGAAGATAAATTTTCCTCTCAAATCCATTGGGAAATTAGATGGCTGTGGAATTGACAAAATTTAGATATTCGGTTTTTATTGGACAAGAGTGTGAAGATTGTAATCGTAGTTGGTTTGAGATTCATTTTGCTAGAGCTGCCCCGTTCGGATACCAAAAGGGTCCGAAGTTGCGAGGCGTTAGTGAGATTCTTTTATTGCAGAGTATATTTCAAGAATCAAAGCTTACAGGCACATTGCCTTCAAGCGACAGAACAACTCTCTACTCCACCAGTTGCCTGCTTTTTTGTTGTACAAAAATATGTAAGTTACTCACAGCACTCACTGAACTTTTTACACTTACAATGTCTTCTCTTCTCTTCT

Coding sequence (CDS)

ATGGACACAACCGATTCAATCACTGCTTTCTCTCATCTTCTTCAACCTCCAGATTTCTCTCACTATGTTGAAATTGATCCCACTGGCAGATATGGAAGAGTACTTTTCTCTCTCTTTCTCTCTAACTATCTTCCCAATTCCCCATCGTTTTTATTCTCTTCTTTCCTGTTTTGCTTGCAGTATAATGAAATCCTCGGCAAAGGAGCTTCCAAGACAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTCCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCATCTCTTAAAAACATTGAAACATAAAAACATAATGAAATTCTATACCTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTCTGAGGCAGTATAGGCTAAAGCATAGGAGAGCTAACATTAGGGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCATTATCTCCATAGCCAAGACCCCCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAGGGAGAAATTAAAATTGGAGACCTTGGTCTTGCTGCTATCCTCCAGAAATCACATGCTGATCATTGTGTTGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAGGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAATCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGATGCCTTGTATAAAGTGAAGGATCCAAGCATGCGACAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTTGAAATTGATATTTGTGAAAGCAAGTTGAAAATTTCAGATTCTCGGAGAGAACTGGACGATTTTGCTTCCACTATAGTGCGGCCTTTTCTTGAACGAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTGGAGGCCTCAGATGAATGGAGGTACCGTTCAGTCCAGAAGGAACGTGATGGAATTGAACTTTTTGAGGACAATGATAATGACCAATTGGAAAATCTTGATAAAAATATTAAGGGGAAAATACGTGAAGATGGCAGCATTGTTTTAAGACTTAGGATTACGGATAAAGAAGGGCTTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACAGCATTAACAGTAGCCACCGAAATGATTGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTTCTTCCTTGGTACCCGAGTGGAAGCCAGGGCCTGGCATAGACGAAACACCTCGCATCTCTTACGATGGTGGCTCTCAAAGCTACAACGCTTCTAATCAACCATCTGATAATATTTTGATAGAGAATAAAGGAAATGGAATCAAACTTTATCAGATTTTGAACTTAAGCACAGATGGGCATGCTTTGGCACATAAACATTTCGAGCAAGAGCAGTTCTCGCTCAAAGCAGATCGGCCTACACAACCTGTGGTAGGGAGCATACCAAATGTGTCAAGCCAGCACTATCAGCCAGATTCTGTCCTCCCTGAAAACCAAGCTCTTAGTTCTCGTAGTTTTAGGCAAAGACATTCTGATGATAATTACAAGAAAACTGACCAATCACTCACTGTTGGGTATAAAGAAAAACTACCAGATAAGAAAGCCACTGTCATTGACACTTCACAAAGAAGCTTATTGGGTTCCCGATCACTGTCCAGGGTTTCATCCTATTGCGAAGATAAATTTTCCTCTCAAATCCATTGGGAAATTAGATGGCTGTGGAATTGA

Protein sequence

MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN
Homology
BLAST of IVF0004590.2 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.0e-175
Identity = 357/669 (53.36%), Postives = 432/669 (64.57%), Query Frame = 0

Query: 17  PDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRA 76
           PD+S +VE+DPTGRYGR                           YNE+LGKGASKTVYRA
Sbjct: 9   PDYSEFVEVDPTGRYGR---------------------------YNEVLGKGASKTVYRA 68

Query: 77  FDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNIN 136
           FDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN+NIN
Sbjct: 69  FDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNIN 128

Query: 137 FVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVN 196
           FVTE+FTSGTLRQYRL+H+R NIRA+K WCRQILRGLHYLHS DPPVIHRDLKCDNIFVN
Sbjct: 129 FVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVN 188

Query: 197 GNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTF 256
           GNQGE+KIGDLGLAAIL+KSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF
Sbjct: 189 GNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTF 248

Query: 257 EYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL 316
           +YPYSEC HP QIYKKV+SGKKPDALYKVKDP ++ F+EKCLA VS R+SARELL DPFL
Sbjct: 249 DYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFL 308

Query: 317 EIDICESKLKISDSR----------RELDDF------ASTIVRPFLEREKRFSSISYSLE 376
            ID  E  L+  D              L D+      +S++ R +       +S S + +
Sbjct: 309 RIDDGEFDLRSVDMEDSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQ 368

Query: 377 AS----------DEWRYRSVQ-KERDGIELFEDNDNDQLE------NLDKNIKGKIREDG 436
            S          + W Y   + +E  GIELFE  +ND  E      N+D  IKGK R+DG
Sbjct: 369 YSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDG 428

Query: 437 SIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISS 496
            + LRLRI DKEG +RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  IDGEISS
Sbjct: 429 GLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANMIDGEISS 488

Query: 497 LVPEWKPGPGIDETPRISYDGGSQSY---NASNQPSDNILIE----NKGNGIKLYQILNL 556
           LVP W+PGP  +E    +    + S      SN+ S   +++    N G       ++  
Sbjct: 489 LVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPG-----ANVIQC 548

Query: 557 STDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQR 616
             +G    H  FE+        R T+  +  +  +  Q         E++ LSS      
Sbjct: 549 CRNGCGETHGRFEEITI-----RETEVRLRELWKLQQQQ--------ESRELSSIDSGHN 608

Query: 617 HSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQ-RSLLGSRSLSRV-SSYCE---DKFSS 641
           HS++     ++   V Y++  P+   +    ++   + GS S S + S YC+   +K  +
Sbjct: 609 HSEE-----EEEEEVLYED--PENMFSCEAGNEINHISGSGSFSFMPSKYCDEPSEKTEN 625

BLAST of IVF0004590.2 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 605.1 bits (1559), Expect = 9.0e-172
Identity = 310/474 (65.40%), Postives = 356/474 (75.11%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAF 77
           D   Y E+DPTGRYGR                           YN++LGKGASKTVYRAF
Sbjct: 13  DLPEYAEVDPTGRYGR---------------------------YNDVLGKGASKTVYRAF 72

Query: 78  DEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINF 137
           DEY+G+EVAWNQVKL +FLQ PEDLERLY EIHLLKTLKH+NIMKFYTSWVD + +NINF
Sbjct: 73  DEYQGMEVAWNQVKLHDFLQSPEDLERLYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINF 132

Query: 138 VTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNG 197
           +TEMFTSGTLRQYR KH R NI AVK WCRQIL GL YLHS DPP+IHRDLKCDNIFVNG
Sbjct: 133 ITEMFTSGTLRQYRQKHMRVNIWAVKHWCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNG 192

Query: 198 NQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFE 257
           NQGE+KIGDLGLAAIL+KSHA HCVGTPEFMAPEVYEE YNELVDIYSFGMCVLEMVTFE
Sbjct: 193 NQGEVKIGDLGLAAILRKSHAVHCVGTPEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFE 252

Query: 258 YPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLE 317
           YPYSEC HPVQIYKKVISG KP+ALYKVKDP +RQFVEKCLA  S RLSARELL DPFL+
Sbjct: 253 YPYSECTHPVQIYKKVISGTKPEALYKVKDPMVRQFVEKCLATASRRLSARELLKDPFLQ 312

Query: 318 IDICESKLKISDSRRELDDFASTIVRPFLERE-KRFSSISYSLEAS--------DEWRYR 377
           +D  +      D    L ++   + +P+LE      S +S  L  S        D W   
Sbjct: 313 VD--DLVFCPGDGDYSLMNY---LRQPYLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCE 372

Query: 378 SVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTK 437
               + DGI+LF  ++++ L N+D  IKG+  EDGSI LRLRI D +G +RNIYFPFD +
Sbjct: 373 DDDIKADGIDLFNGHEDEPLGNVDITIKGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIE 432

Query: 438 NDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISY 483
            DTAL+VATEM+AELDITD +V +IAE IDGE+S+LVP+W+PGPGI+E+   +Y
Sbjct: 433 ADTALSVATEMVAELDITDHEVTRIAEMIDGEVSALVPDWRPGPGIEESQDTTY 454

BLAST of IVF0004590.2 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 9.0e-172
Identity = 310/474 (65.40%), Postives = 356/474 (75.11%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAF 77
           D   Y E+DPTGRYGR                           YN++LGKGASKTVYRAF
Sbjct: 13  DLPEYAEVDPTGRYGR---------------------------YNDVLGKGASKTVYRAF 72

Query: 78  DEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINF 137
           DEY+G+EVAWNQVKL +FLQ PEDLERLY EIHLLKTLKH+NIMKFYTSWVD + +NINF
Sbjct: 73  DEYQGMEVAWNQVKLHDFLQSPEDLERLYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINF 132

Query: 138 VTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNG 197
           +TEMFTSGTLRQYR KH R NI AVK WCRQIL GL YLHS DPP+IHRDLKCDNIFVNG
Sbjct: 133 ITEMFTSGTLRQYRQKHMRVNIWAVKHWCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNG 192

Query: 198 NQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFE 257
           NQGE+KIGDLGLAAIL+KSHA HCVGTPEFMAPEVYEE YNELVDIYSFGMCVLEMVTFE
Sbjct: 193 NQGEVKIGDLGLAAILRKSHAVHCVGTPEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFE 252

Query: 258 YPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLE 317
           YPYSEC HPVQIYKKVISG KP+ALYKVKDP +RQFVEKCLA  S RLSARELL DPFL+
Sbjct: 253 YPYSECTHPVQIYKKVISGTKPEALYKVKDPMVRQFVEKCLATASRRLSARELLKDPFLQ 312

Query: 318 IDICESKLKISDSRRELDDFASTIVRPFLERE-KRFSSISYSLEAS--------DEWRYR 377
           +D  +      D    L ++   + +P+LE      S +S  L  S        D W   
Sbjct: 313 VD--DLVFCPGDGDYSLMNY---LRQPYLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCE 372

Query: 378 SVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTK 437
               + DGI+LF  ++++ L N+D  IKG+  EDGSI LRLRI D +G +RNIYFPFD +
Sbjct: 373 DDDIKADGIDLFNGHEDEPLGNVDITIKGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIE 432

Query: 438 NDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISY 483
            DTAL+VATEM+AELDITD +V +IAE IDGE+S+LVP+W+PGPGI+E+   +Y
Sbjct: 433 ADTALSVATEMVAELDITDHEVTRIAEMIDGEVSALVPDWRPGPGIEESQDTTY 454

BLAST of IVF0004590.2 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.2e-160
Identity = 299/467 (64.03%), Postives = 351/467 (75.16%), Query Frame = 0

Query: 11  SHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGAS 70
           SHL    D+S YVE+DPTGRYGR                           YNE+LGKG+S
Sbjct: 6   SHL--ESDYSEYVEVDPTGRYGR---------------------------YNEVLGKGSS 65

Query: 71  KTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDT 130
           KTVYR FDEY+GIEVAWNQVKL +FLQ P++LERLY EIHLLKTLKHK+IMKFY SWVDT
Sbjct: 66  KTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELERLYCEIHLLKTLKHKSIMKFYASWVDT 125

Query: 131 ANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKC 190
            N+NINFVTEMFTSGTLRQYRLKH+R NIRAVK WCRQILRGL+YLH+ DPPVIHRDLKC
Sbjct: 126 DNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKNWCRQILRGLNYLHTHDPPVIHRDLKC 185

Query: 191 DNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCV 250
           DNIF+NGNQGE+KIGDLGLAA LQ SHA HCVGTPEFMAPEVY+E YN+LVDIYSFGMCV
Sbjct: 186 DNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGTPEFMAPEVYKEEYNQLVDIYSFGMCV 245

Query: 251 LEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSAREL 310
           LEMVTF+YPYSEC+HP QIYK+VISGKKPD L KVKDP +R F+EKCLA VS RLSA EL
Sbjct: 246 LEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDKVKDPEVRGFIEKCLATVSLRLSACEL 305

Query: 311 LSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSV 370
           L D FL ID  ES ++  +S + L D A T +R        +S+  YSL   ++W Y   
Sbjct: 306 LDDHFLCID--ESDMRRVESEKGLIDEAGTPLR-HSYHIPHYSNGYYSLYNQNQWDYNGD 365

Query: 371 QK-ERDGIELFE-DNDNDQLE------NLDKNIKGKIREDG-SIVLRLRITDKEGLIRNI 430
           +  E   I+L E  ND+D+ E      ++D +IKGK R++G  + LRL+  +KEG +RNI
Sbjct: 366 ETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIKGKRRDNGDGLFLRLKTVNKEGCVRNI 425

Query: 431 YFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEW 469
           YFPFD + DTA++VA EM+ EL++ D+DV KIA  IDGEI+SLVP W
Sbjct: 426 YFPFDIETDTAISVAREMVEELEMDDRDVTKIANMIDGEIASLVPNW 440

BLAST of IVF0004590.2 vs. ExPASy Swiss-Prot
Match: Q8S8Y9 (Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 2.1e-152
Identity = 307/592 (51.86%), Postives = 380/592 (64.19%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAF 77
           D S +VEIDP+GRYGR                           Y+EILGKGASKTVYRAF
Sbjct: 10  DCSVFVEIDPSGRYGR---------------------------YDEILGKGASKTVYRAF 69

Query: 78  DEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINF 137
           DEYEGIEVAWNQVKL NF + PE+LE+ + EIHLLKTL H+NIMKFYTSWVDT N +INF
Sbjct: 70  DEYEGIEVAWNQVKLRNFTRNPEELEKFFREIHLLKTLNHQNIMKFYTSWVDTNNLSINF 129

Query: 138 VTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNG 197
           VTE+FTSGTLRQYRL+HRR NIRAVK+WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NG
Sbjct: 130 VTELFTSGTLRQYRLRHRRVNIRAVKQWCKQILKGLLYLHSRSPPIIHRDLKCDNIFING 189

Query: 198 NQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFE 257
           NQGE+KIGDLGLAAIL+KSHA  CVGTPEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+
Sbjct: 190 NQGEVKIGDLGLAAILRKSHAVRCVGTPEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFD 249

Query: 258 YPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLE 317
           YPYSEC HP QIYKKV SGKKP+A Y VKDP +R+FVEKCLA V+CRL+A ELL DPFL+
Sbjct: 250 YPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQDPFLQ 309

Query: 318 IDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGI 377
            D              +D F    +  +   ++    + + L     +  +    +   I
Sbjct: 310 DD-------------NMDGFVMRPIDYYNGYDETGVFLRHPLIDDPLYHDQFESSQICEI 369

Query: 378 ELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVAT 437
           +LF ++D D   ++D +IKGK   D  I LRLRI+D EG IRNIYFPF+T  DTA +VA 
Sbjct: 370 DLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAV 429

Query: 438 EMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSD 497
           EM++ELDIT+QDV KIAE ID EI++LVP+WK             +    S N +N  ++
Sbjct: 430 EMVSELDITNQDVAKIAEMIDAEIAALVPDWK-------------NDTESSQNVNNNKNN 489

Query: 498 N---ILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQ 557
           N      E   NG     I    + G    H H E   F    D+    V G   ++   
Sbjct: 490 NTAGFCGECASNG----YIQETVSSGEKSHHNHHE---FDSSEDKSCSSVHGRFADMWGL 535

Query: 558 HYQPDSVLPENQALSSRSFRQ-RHSDDNYKKTDQSLTVGYKEKLPDKKATVI 606
               +S   + +  SSR  R  R S++  ++  + L   +K +L   +   I
Sbjct: 550 R---ESYSDDGEKQSSRKVRSGRWSENEMRRELRWLKARHKIQLMKMRGQTI 535

BLAST of IVF0004590.2 vs. ExPASy TrEMBL
Match: A0A1S3CI45 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103501021 PE=4 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 613/642 (95.48%), Postives = 613/642 (95.48%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR                           
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD
Sbjct: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE
Sbjct: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
           ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN
Sbjct: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP
Sbjct: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDK 600
           TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRH DDNYKKTDQSL VGYKEKLPDK
Sbjct: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHYDDNYKKTDQSLAVGYKEKLPDK 600

Query: 601 KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 643
           KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 615

BLAST of IVF0004590.2 vs. ExPASy TrEMBL
Match: A0A0A0M0J2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G695390 PE=4 SV=1)

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 592/643 (92.07%), Postives = 596/643 (92.69%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR                           
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           Y+EILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YDEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD
Sbjct: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKP ALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCRLSARELLSDPFLEID CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE
Sbjct: 301 VSCRLSARELLSDPFLEIDGCESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
            SDEWRYRSVQKE DGIELFEDNDNDQL +LD NIKGKIREDGSIVLRLRITDKEGLIRN
Sbjct: 361 GSDEWRYRSVQKEPDGIELFEDNDNDQLVSLDNNIKGKIREDGSIVLRLRITDKEGLIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGGSQSYNA NQPSDNILIENKGNGIKLYQILNLSTDGHALAH+HFEQEQFSLKADRP
Sbjct: 481 SYDGGSQSYNACNQPSDNILIENKGNGIKLYQILNLSTDGHALAHEHFEQEQFSLKADRP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGY-KEKLPD 600
           TQ      PNVSSQHYQPDSVL ENQALSS SFRQRHSDDNYKK DQSLTVGY KEKLP 
Sbjct: 541 TQ------PNVSSQHYQPDSVLNENQALSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPV 600

Query: 601 KKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 643
            KATVIDTSQRSLLGSRSLS VSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 NKATVIDTSQRSLLGSRSLSTVSSYCEDKFSSQIHWEIRWLWN 610

BLAST of IVF0004590.2 vs. ExPASy TrEMBL
Match: A0A5D3E1C3 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3607G00400 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.9e-290
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 141 MFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQG 200
           MFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQG
Sbjct: 1   MFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQG 60

Query: 201 EIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPY 260
           EIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPY
Sbjct: 61  EIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPY 120

Query: 261 SECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDI 320
           SECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDI
Sbjct: 121 SECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDI 180

Query: 321 CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELF 380
           CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELF
Sbjct: 181 CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELF 240

Query: 381 EDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMI 440
           EDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMI
Sbjct: 241 EDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMI 300

Query: 441 AELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNIL 500
           AELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNIL
Sbjct: 301 AELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNIL 360

Query: 501 IENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDS 560
           IENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDS
Sbjct: 361 IENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDS 420

Query: 561 VLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQRSLLGSRSLSR 620
           VLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQRSLLGSRSLSR
Sbjct: 421 VLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQRSLLGSRSLSR 480

Query: 621 VSSYCEDKFSSQIHWEIRWLWN 643
           VSSYCEDKFSSQIHWEIRWLWN
Sbjct: 481 VSSYCEDKFSSQIHWEIRWLWN 502

BLAST of IVF0004590.2 vs. ExPASy TrEMBL
Match: A0A6J1KJY6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495945 PE=4 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 4.1e-284
Identity = 512/643 (79.63%), Postives = 544/643 (84.60%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           M+T DSIT FS+LLQPPDFS YVEIDPTGRYGR                           
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNI
Sbjct: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTAN+NINFVTEMFTSGTLRQYRLKHRR NIRAVKRWCRQILRGL YLHSQ+
Sbjct: 121 MKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQN 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNI VNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNILVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCR SARELLSDPFL+ID CESKL++S   REL+  A T   PFLER+  FSSI+Y LE
Sbjct: 301 VSCRPSARELLSDPFLQIDDCESKLQLSGCPRELNGIAFTTAHPFLERQGSFSSINYCLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
           ASDEWR RSVQ E DG ELFE++D+DQLENLD +IKGKIREDGSIVLRLRI DKEG IRN
Sbjct: 361 ASDEWRCRSVQTEPDGFELFENDDDDQLENLDNSIKGKIREDGSIVLRLRIADKEGRIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEI+SLVPEWK GPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKSGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGG QS++A+N   D+ LI N  NG KLYQILNLST   A  H+ F+Q+  SLKAD P
Sbjct: 481 SYDGGCQSHDAANHSCDHSLIGNNQNGEKLYQILNLSTVERASVHELFKQD--SLKADPP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVG-YKEKLPD 600
            +PVV S P VSSQHYQP+SVL EN AL S SFR  HS+ N+KK  QS+ VG  KEK+ D
Sbjct: 541 PEPVVESPPKVSSQHYQPNSVLLENPALGSHSFRHSHSNQNFKKIGQSIIVGDDKEKILD 600

Query: 601 KKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 643
           K A +  TS RSLLGSRSLS V SY EDKF+SQIH EIRWLWN
Sbjct: 601 KNA-IARTSLRSLLGSRSLSTVPSYREDKFASQIHREIRWLWN 613

BLAST of IVF0004590.2 vs. ExPASy TrEMBL
Match: A0A6J1F099 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441166 PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.9e-284
Identity = 511/643 (79.47%), Postives = 543/643 (84.45%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           M+T DSIT FS+LLQPPDFS YVEIDPTGRYGR                           
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTAN+NINFVTEMFTSGTLRQYRLKHRR NIRAVKRWCRQILRGL YLHSQ+
Sbjct: 121 MKFYTSWVDTANRNINFVTEMFTSGTLRQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQN 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNI VNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNILVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKV+SGKKPDALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCR SARELLSDPFL+ID CESKL++S   RELD  A T   PFLER+  FSSI+Y LE
Sbjct: 301 VSCRPSARELLSDPFLQIDDCESKLQLSGCPRELDGIAFTTAHPFLERQGSFSSINYRLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
           ASDEWR  SVQ E DG ELFE++D+DQLENLD +IKGKIREDGSIVLRLRI DKEG IRN
Sbjct: 361 ASDEWRCHSVQTEPDGFELFENDDDDQLENLDNSIKGKIREDGSIVLRLRIADKEGRIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEI+SLVPEWK GPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPEWKSGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDG  QS++A+N   D+ LI N  NG KLYQILNLST   A  H+ F+Q+  SLKADRP
Sbjct: 481 SYDGDCQSHDAANHSCDHSLIGNNQNGEKLYQILNLSTVERASVHELFKQD--SLKADRP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVG-YKEKLPD 600
            +PVV S P VSSQHYQP+SVL E+ AL S SFR  HS+ N+KK  QS+ VG  KEK+ D
Sbjct: 541 PEPVVESPPKVSSQHYQPNSVLLEDPALGSHSFRHSHSNQNFKKIGQSIIVGDDKEKILD 600

Query: 601 KKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 643
           K A +  TS RSLLGSRSLS V SY EDKF+SQIH EIRWLWN
Sbjct: 601 KNA-IARTSLRSLLGSRSLSTVPSYREDKFASQIHREIRWLWN 613

BLAST of IVF0004590.2 vs. NCBI nr
Match: XP_031736005.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis sativus])

HSP 1 Score: 1224 bits (3168), Expect = 0.0
Identity = 618/644 (95.96%), Postives = 623/644 (96.74%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSL-FLSNYLPNSPSFLFSSFLFCL 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSL FLS+YLPNSPSFLFSSFLFCL
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLLFLSHYLPNSPSFLFSSFLFCL 60

Query: 61  QYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120
           QY+EILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN
Sbjct: 61  QYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120

Query: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ 180
           IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ
Sbjct: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ 180

Query: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNE 240
           DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNE
Sbjct: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNE 240

Query: 241 LVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLA 300
           LVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKP ALYKVKDPSMRQFVEKCLA
Sbjct: 241 LVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLA 300

Query: 301 PVSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL 360
           PVSCRLSARELLSDPFLEID CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL
Sbjct: 301 PVSCRLSARELLSDPFLEIDGCESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL 360

Query: 361 EASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIR 420
           E SDEWRYRSVQKE DGIELFEDNDNDQL +LD NIKGKIREDGSIVLRLRITDKEGLIR
Sbjct: 361 EGSDEWRYRSVQKEPDGIELFEDNDNDQLVSLDNNIKGKIREDGSIVLRLRITDKEGLIR 420

Query: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR 480
           NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR
Sbjct: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR 480

Query: 481 ISYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADR 540
           ISYDGGSQSYNA NQPSDNILIENKGNGIKLYQILNLSTDGHALAH+HFEQEQFSLKADR
Sbjct: 481 ISYDGGSQSYNACNQPSDNILIENKGNGIKLYQILNLSTDGHALAHEHFEQEQFSLKADR 540

Query: 541 PTQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGY-KEKLP 600
           PTQP      NVSSQHYQPDSVL ENQALSS SFRQRHSDDNYKK DQSLTVGY KEKLP
Sbjct: 541 PTQP------NVSSQHYQPDSVLNENQALSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLP 600

Query: 601 DKKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 642
             KATVIDTSQRSLLGSRSLS VSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 VNKATVIDTSQRSLLGSRSLSTVSSYCEDKFSSQIHWEIRWLWN 638

BLAST of IVF0004590.2 vs. NCBI nr
Match: XP_008462723.1 (PREDICTED: probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis melo])

HSP 1 Score: 1222 bits (3161), Expect = 0.0
Identity = 613/642 (95.48%), Postives = 613/642 (95.48%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR                           
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD
Sbjct: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE
Sbjct: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
           ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN
Sbjct: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP
Sbjct: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGYKEKLPDK 600
           TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRH DDNYKKTDQSL VGYKEKLPDK
Sbjct: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHYDDNYKKTDQSLAVGYKEKLPDK 600

Query: 601 KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 642
           KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 KATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 615

BLAST of IVF0004590.2 vs. NCBI nr
Match: XP_004142568.1 (probable serine/threonine-protein kinase WNK9 isoform X2 [Cucumis sativus] >KGN66804.1 hypothetical protein Csa_006948 [Cucumis sativus])

HSP 1 Score: 1165 bits (3013), Expect = 0.0
Identity = 592/643 (92.07%), Postives = 596/643 (92.69%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR                           
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           Y+EILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YDEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD
Sbjct: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKP ALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCRLSARELLSDPFLEID CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE
Sbjct: 301 VSCRLSARELLSDPFLEIDGCESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
            SDEWRYRSVQKE DGIELFEDNDNDQL +LD NIKGKIREDGSIVLRLRITDKEGLIRN
Sbjct: 361 GSDEWRYRSVQKEPDGIELFEDNDNDQLVSLDNNIKGKIREDGSIVLRLRITDKEGLIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGGSQSYNA NQPSDNILIENKGNGIKLYQILNLSTDGHALAH+HFEQEQFSLKADRP
Sbjct: 481 SYDGGSQSYNACNQPSDNILIENKGNGIKLYQILNLSTDGHALAHEHFEQEQFSLKADRP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGY-KEKLPD 600
           TQP      NVSSQHYQPDSVL ENQALSS SFRQRHSDDNYKK DQSLTVGY KEKLP 
Sbjct: 541 TQP------NVSSQHYQPDSVLNENQALSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPV 600

Query: 601 KKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 642
            KATVIDTSQRSLLGSRSLS VSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 NKATVIDTSQRSLLGSRSLSTVSSYCEDKFSSQIHWEIRWLWN 610

BLAST of IVF0004590.2 vs. NCBI nr
Match: XP_031736006.1 (probable serine/threonine-protein kinase WNK9 isoform X3 [Cucumis sativus])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 583/644 (90.53%), Postives = 588/644 (91.30%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSL-FLSNYLPNSPSFLFSSFLFCL 60
           MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSL FLS+YLPNSPSFLFSSFLFCL
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLLFLSHYLPNSPSFLFSSFLFCL 60

Query: 61  QYNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120
           QY+EILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN
Sbjct: 61  QYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120

Query: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ 180
           IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ
Sbjct: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ 180

Query: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNE 240
           DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNE
Sbjct: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNE 240

Query: 241 LVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLA 300
           L                                   GKKP ALYKVKDPSMRQFVEKCLA
Sbjct: 241 L-----------------------------------GKKPAALYKVKDPSMRQFVEKCLA 300

Query: 301 PVSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL 360
           PVSCRLSARELLSDPFLEID CESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL
Sbjct: 301 PVSCRLSARELLSDPFLEIDGCESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL 360

Query: 361 EASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIR 420
           E SDEWRYRSVQKE DGIELFEDNDNDQL +LD NIKGKIREDGSIVLRLRITDKEGLIR
Sbjct: 361 EGSDEWRYRSVQKEPDGIELFEDNDNDQLVSLDNNIKGKIREDGSIVLRLRITDKEGLIR 420

Query: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR 480
           NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR
Sbjct: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR 480

Query: 481 ISYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADR 540
           ISYDGGSQSYNA NQPSDNILIENKGNGIKLYQILNLSTDGHALAH+HFEQEQFSLKADR
Sbjct: 481 ISYDGGSQSYNACNQPSDNILIENKGNGIKLYQILNLSTDGHALAHEHFEQEQFSLKADR 540

Query: 541 PTQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGY-KEKLP 600
           PTQP      NVSSQHYQPDSVL ENQALSS SFRQRHSDDNYKK DQSLTVGY KEKLP
Sbjct: 541 PTQP------NVSSQHYQPDSVLNENQALSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLP 600

Query: 601 DKKATVIDTSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 642
             KATVIDTSQRSLLGSRSLS VSSYCEDKFSSQIHWEIRWLWN
Sbjct: 601 VNKATVIDTSQRSLLGSRSLSTVSSYCEDKFSSQIHWEIRWLWN 603

BLAST of IVF0004590.2 vs. NCBI nr
Match: XP_038880702.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 547/644 (84.94%), Postives = 568/644 (88.20%), Query Frame = 0

Query: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQ 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGR                           
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGR--------------------------- 60

Query: 61  YNEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120
           YNEILGKGASK VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI
Sbjct: 61  YNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNI 120

Query: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQD 180
           MKFYTSWVDTANKNINFVTEMFTSGTLRQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQD
Sbjct: 121 MKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQD 180

Query: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNEL 240
           PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAIL+KSHADHCVGTPEFMAPEVYEEAYNEL
Sbjct: 181 PPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL 240

Query: 241 VDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAP 300
           VDIYSFGMCVLEMVTFEYPYSEC+HPVQIYKKVISGKKP ALYKVKDPSMRQFVEKCLAP
Sbjct: 241 VDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAP 300

Query: 301 VSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLE 360
           VSCRLSARELLSDPFL++D  ESK KIS+SR ELDDFASTI  PFLE+EKRFSSISYS E
Sbjct: 301 VSCRLSARELLSDPFLQVDDGESKSKISNSRGELDDFASTIAHPFLEQEKRFSSISYSFE 360

Query: 361 ASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRN 420
            SDEWR  +VQKE DGIELFED+DND LENLD +IKGKIREDGSIVLRLRI DKEG IRN
Sbjct: 361 TSDEWRCHTVQKEADGIELFEDDDNDILENLDNSIKGKIREDGSIVLRLRIADKEGRIRN 420

Query: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRI 480
           IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEI+SLVP+WKPGPGIDETPRI
Sbjct: 421 IYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPDWKPGPGIDETPRI 480

Query: 481 SYDGGSQSYNASNQPSDNILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRP 540
           SYDGGS+SYN SNQPSDN LIENKGNG KLYQILN S DG AL   HFEQ QFSLKAD+P
Sbjct: 481 SYDGGSRSYNDSNQPSDNSLIENKGNGAKLYQILNCSIDGRALVQGHFEQGQFSLKADQP 540

Query: 541 TQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQRHSDDNYKKTDQSLTVGY-KEKLPD 600
           TQ VV S P VSSQH Q +S L ENQALSS SFRQRHS +NYKKTDQS+TVGY KEK+PD
Sbjct: 541 TQSVVESRPKVSSQHNQSNSALHENQALSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPD 600

Query: 601 KKATVID-TSQRSLLGSRSLSRVSSYCEDKFSSQIHWEIRWLWN 642
            KATVID TS RSLLGSRSLS V SYCEDKF+SQIHWEIRWLWN
Sbjct: 601 TKATVIDRTSLRSLLGSRSLSTVPSYCEDKFASQIHWEIRWLWN 617

BLAST of IVF0004590.2 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 618.2 bits (1593), Expect = 7.3e-177
Identity = 357/669 (53.36%), Postives = 432/669 (64.57%), Query Frame = 0

Query: 17  PDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRA 76
           PD+S +VE+DPTGRYGR                           YNE+LGKGASKTVYRA
Sbjct: 9   PDYSEFVEVDPTGRYGR---------------------------YNEVLGKGASKTVYRA 68

Query: 77  FDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNIN 136
           FDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN+NIN
Sbjct: 69  FDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNIN 128

Query: 137 FVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVN 196
           FVTE+FTSGTLRQYRL+H+R NIRA+K WCRQILRGLHYLHS DPPVIHRDLKCDNIFVN
Sbjct: 129 FVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVN 188

Query: 197 GNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTF 256
           GNQGE+KIGDLGLAAIL+KSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF
Sbjct: 189 GNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTF 248

Query: 257 EYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL 316
           +YPYSEC HP QIYKKV+SGKKPDALYKVKDP ++ F+EKCLA VS R+SARELL DPFL
Sbjct: 249 DYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFL 308

Query: 317 EIDICESKLKISDSR----------RELDDF------ASTIVRPFLEREKRFSSISYSLE 376
            ID  E  L+  D              L D+      +S++ R +       +S S + +
Sbjct: 309 RIDDGEFDLRSVDMEDSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQ 368

Query: 377 AS----------DEWRYRSVQ-KERDGIELFEDNDNDQLE------NLDKNIKGKIREDG 436
            S          + W Y   + +E  GIELFE  +ND  E      N+D  IKGK R+DG
Sbjct: 369 YSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIKGKRRDDG 428

Query: 437 SIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISS 496
            + LRLRI DKEG +RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  IDGEISS
Sbjct: 429 GLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANMIDGEISS 488

Query: 497 LVPEWKPGPGIDETPRISYDGGSQSY---NASNQPSDNILIE----NKGNGIKLYQILNL 556
           LVP W+PGP  +E    +    + S      SN+ S   +++    N G       ++  
Sbjct: 489 LVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPG-----ANVIQC 548

Query: 557 STDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQALSSRSFRQR 616
             +G    H  FE+        R T+  +  +  +  Q         E++ LSS      
Sbjct: 549 CRNGCGETHGRFEEITI-----RETEVRLRELWKLQQQQ--------ESRELSSIDSGHN 608

Query: 617 HSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQ-RSLLGSRSLSRV-SSYCE---DKFSS 641
           HS++     ++   V Y++  P+   +    ++   + GS S S + S YC+   +K  +
Sbjct: 609 HSEE-----EEEEEVLYED--PENMFSCEAGNEINHISGSGSFSFMPSKYCDEPSEKTEN 625

BLAST of IVF0004590.2 vs. TAIR 10
Match: AT3G04910.2 (with no lysine (K) kinase 1 )

HSP 1 Score: 575.1 bits (1481), Expect = 7.1e-164
Identity = 332/613 (54.16%), Postives = 404/613 (65.91%), Query Frame = 0

Query: 73  VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN 132
           +YRAFDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN
Sbjct: 15  LYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTAN 74

Query: 133 KNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDN 192
           +NINFVTE+FTSGTLRQYRL+H+R NIRA+K WCRQILRGLHYLHS DPPVIHRDLKCDN
Sbjct: 75  RNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDN 134

Query: 193 IFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLE 252
           IFVNGNQGE+KIGDLGLAAIL+KSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LE
Sbjct: 135 IFVNGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILE 194

Query: 253 MVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 312
           MVTF+YPYSEC HP QIYKKV+SGKKPDALYKVKDP ++ F+EKCLA VS R+SARELL 
Sbjct: 195 MVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLD 254

Query: 313 DPFLEIDICESKLKISDSR----------RELDDF------ASTIVRPFLEREKRFSSIS 372
           DPFL ID  E  L+  D              L D+      +S++ R +       +S S
Sbjct: 255 DPFLRIDDGEFDLRSVDMEDSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSS 314

Query: 373 YSLEAS----------DEWRYRSVQ-KERDGIELFEDNDNDQLE------NLDKNIKGKI 432
            + + S          + W Y   + +E  GIELFE  +ND  E      N+D  IKGK 
Sbjct: 315 LNRQYSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIKGKR 374

Query: 433 REDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDG 492
           R+DG + LRLRI DKEG +RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  IDG
Sbjct: 375 RDDGGLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANMIDG 434

Query: 493 EISSLVPEWKPGPGIDETPRISYDGGSQSY---NASNQPSDNILIE----NKGNGIKLYQ 552
           EISSLVP W+PGP  +E    +    + S      SN+ S   +++    N G       
Sbjct: 435 EISSLVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPG-----AN 494

Query: 553 ILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQALSSRS 612
           ++    +G    H  FE+        R T+  +  +  +  Q         E++ LSS  
Sbjct: 495 VIQCCRNGCGETHGRFEEITI-----RETEVRLRELWKLQQQQ--------ESRELSSID 554

Query: 613 FRQRHSDDNYKKTDQSLTVGYKEKLPDKKATVIDTSQ-RSLLGSRSLSRV-SSYCE---D 641
               HS++     ++   V Y++  P+   +    ++   + GS S S + S YC+   +
Sbjct: 555 SGHNHSEE-----EEEEEVLYED--PENMFSCEAGNEINHISGSGSFSFMPSKYCDEPSE 602

BLAST of IVF0004590.2 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 568.2 bits (1463), Expect = 8.6e-162
Identity = 299/467 (64.03%), Postives = 351/467 (75.16%), Query Frame = 0

Query: 11  SHLLQPPDFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGAS 70
           SHL    D+S YVE+DPTGRYGR                           YNE+LGKG+S
Sbjct: 6   SHL--ESDYSEYVEVDPTGRYGR---------------------------YNEVLGKGSS 65

Query: 71  KTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDT 130
           KTVYR FDEY+GIEVAWNQVKL +FLQ P++LERLY EIHLLKTLKHK+IMKFY SWVDT
Sbjct: 66  KTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELERLYCEIHLLKTLKHKSIMKFYASWVDT 125

Query: 131 ANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKC 190
            N+NINFVTEMFTSGTLRQYRLKH+R NIRAVK WCRQILRGL+YLH+ DPPVIHRDLKC
Sbjct: 126 DNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKNWCRQILRGLNYLHTHDPPVIHRDLKC 185

Query: 191 DNIFVNGNQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCV 250
           DNIF+NGNQGE+KIGDLGLAA LQ SHA HCVGTPEFMAPEVY+E YN+LVDIYSFGMCV
Sbjct: 186 DNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGTPEFMAPEVYKEEYNQLVDIYSFGMCV 245

Query: 251 LEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSAREL 310
           LEMVTF+YPYSEC+HP QIYK+VISGKKPD L KVKDP +R F+EKCLA VS RLSA EL
Sbjct: 246 LEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDKVKDPEVRGFIEKCLATVSLRLSACEL 305

Query: 311 LSDPFLEIDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSV 370
           L D FL ID  ES ++  +S + L D A T +R        +S+  YSL   ++W Y   
Sbjct: 306 LDDHFLCID--ESDMRRVESEKGLIDEAGTPLR-HSYHIPHYSNGYYSLYNQNQWDYNGD 365

Query: 371 QK-ERDGIELFE-DNDNDQLE------NLDKNIKGKIREDG-SIVLRLRITDKEGLIRNI 430
           +  E   I+L E  ND+D+ E      ++D +IKGK R++G  + LRL+  +KEG +RNI
Sbjct: 366 ETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIKGKRRDNGDGLFLRLKTVNKEGCVRNI 425

Query: 431 YFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEW 469
           YFPFD + DTA++VA EM+ EL++ D+DV KIA  IDGEI+SLVP W
Sbjct: 426 YFPFDIETDTAISVAREMVEELEMDDRDVTKIANMIDGEIASLVPNW 440

BLAST of IVF0004590.2 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 540.8 bits (1392), Expect = 1.5e-153
Identity = 307/592 (51.86%), Postives = 380/592 (64.19%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAF 77
           D S +VEIDP+GRYGR                           Y+EILGKGASKTVYRAF
Sbjct: 10  DCSVFVEIDPSGRYGR---------------------------YDEILGKGASKTVYRAF 69

Query: 78  DEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINF 137
           DEYEGIEVAWNQVKL NF + PE+LE+ + EIHLLKTL H+NIMKFYTSWVDT N +INF
Sbjct: 70  DEYEGIEVAWNQVKLRNFTRNPEELEKFFREIHLLKTLNHQNIMKFYTSWVDTNNLSINF 129

Query: 138 VTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNG 197
           VTE+FTSGTLRQYRL+HRR NIRAVK+WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NG
Sbjct: 130 VTELFTSGTLRQYRLRHRRVNIRAVKQWCKQILKGLLYLHSRSPPIIHRDLKCDNIFING 189

Query: 198 NQGEIKIGDLGLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFE 257
           NQGE+KIGDLGLAAIL+KSHA  CVGTPEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+
Sbjct: 190 NQGEVKIGDLGLAAILRKSHAVRCVGTPEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFD 249

Query: 258 YPYSECNHPVQIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLE 317
           YPYSEC HP QIYKKV SGKKP+A Y VKDP +R+FVEKCLA V+CRL+A ELL DPFL+
Sbjct: 250 YPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQDPFLQ 309

Query: 318 IDICESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGI 377
            D              +D F    +  +   ++    + + L     +  +    +   I
Sbjct: 310 DD-------------NMDGFVMRPIDYYNGYDETGVFLRHPLIDDPLYHDQFESSQICEI 369

Query: 378 ELFEDNDNDQLENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVAT 437
           +LF ++D D   ++D +IKGK   D  I LRLRI+D EG IRNIYFPF+T  DTA +VA 
Sbjct: 370 DLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAV 429

Query: 438 EMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSD 497
           EM++ELDIT+QDV KIAE ID EI++LVP+WK             +    S N +N  ++
Sbjct: 430 EMVSELDITNQDVAKIAEMIDAEIAALVPDWK-------------NDTESSQNVNNNKNN 489

Query: 498 N---ILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQ 557
           N      E   NG     I    + G    H H E   F    D+    V G   ++   
Sbjct: 490 NTAGFCGECASNG----YIQETVSSGEKSHHNHHE---FDSSEDKSCSSVHGRFADMWGL 535

Query: 558 HYQPDSVLPENQALSSRSFRQ-RHSDDNYKKTDQSLTVGYKEKLPDKKATVI 606
               +S   + +  SSR  R  R S++  ++  + L   +K +L   +   I
Sbjct: 550 R---ESYSDDGEKQSSRKVRSGRWSENEMRRELRWLKARHKIQLMKMRGQTI 535

BLAST of IVF0004590.2 vs. TAIR 10
Match: AT3G22420.2 (with no lysine (K) kinase 2 )

HSP 1 Score: 511.1 bits (1315), Expect = 1.3e-144
Identity = 307/651 (47.16%), Postives = 381/651 (58.53%), Query Frame = 0

Query: 18  DFSHYVEIDPTGRYGRVLFSLFLSNYLPNSPSFLFSSFLFCLQYNEILGKGASKTVYRAF 77
           D S +VEIDP+GRYGR                           Y+EILGKGASKTVYRAF
Sbjct: 10  DCSVFVEIDPSGRYGR---------------------------YDEILGKGASKTVYRAF 69

Query: 78  DEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINF 137
           DEYEGIEVAWNQVKL NF + PE+LE+ + EIHLLKTL H+NIMKFYTSWVDT N +INF
Sbjct: 70  DEYEGIEVAWNQVKLRNFTRNPEELEKFFREIHLLKTLNHQNIMKFYTSWVDTNNLSINF 129

Query: 138 VTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNG 197
           VTE+FTSGTLRQYRL+HRR NIRAVK+WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NG
Sbjct: 130 VTELFTSGTLRQYRLRHRRVNIRAVKQWCKQILKGLLYLHSRSPPIIHRDLKCDNIFING 189

Query: 198 NQGEIKIGDLGLAAILQKSHADHCV--------------------------------GTP 257
           NQGE+KIGDLGLAAIL+KSHA  CV                                GTP
Sbjct: 190 NQGEVKIGDLGLAAILRKSHAVRCVGTSKPSHHWNFIALIMFFTTLDLPLLCLCVVKGTP 249

Query: 258 EFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPDALYKV 317
           EFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC HP QIYKKV SGKKP+A Y V
Sbjct: 250 EFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLV 309

Query: 318 KDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKISDSRRELDDFASTIVRPF 377
           KDP +R+FVEKCLA V+CRL+A ELL DPFL+ D              +D F    +  +
Sbjct: 310 KDPEVREFVEKCLANVTCRLTALELLQDPFLQDD-------------NMDGFVMRPIDYY 369

Query: 378 LEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQLENLDKNIKGKIREDGSI 437
              ++    + + L     +  +    +   I+LF ++D D   ++D +IKGK   D  I
Sbjct: 370 NGYDETGVFLRHPLIDDPLYHDQFESSQICEIDLFANDDED---HVDISIKGKRNGDDGI 429

Query: 438 VLRLRITDKEGL---------------------------IRNIYFPFDTKNDTALTVATE 497
            LRLRI+D EG+                           IRNIYFPF+T  DTA +VA E
Sbjct: 430 FLRLRISDAEGIVSIFFDSFKIIGLKNVTRFRVLGNVGRIRNIYFPFETAIDTAWSVAVE 489

Query: 498 MIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDN 557
           M++ELDIT+QDV KIAE ID EI++LVP+WK             +    S N +N  ++N
Sbjct: 490 MVSELDITNQDVAKIAEMIDAEIAALVPDWK-------------NDTESSQNVNNNKNNN 549

Query: 558 ---ILIENKGNGIKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQH 606
                 E   NG     I    + G    H H E   F    D+    V G   ++    
Sbjct: 550 TAGFCGECASNG----YIQETVSSGEKSHHNHHE---FDSSEDKSCSSVHGRFADMWGLR 594

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CAV61.0e-17553.36Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
A2YMV69.0e-17265.40Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3... [more]
Q0D5989.0e-17265.40Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX... [more]
Q2V3381.2e-16064.03Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y92.1e-15251.86Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3CI450.0e+0095.48Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0M0J20.0e+0092.07Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A5D3E1C34.9e-290100.00Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1KJY64.1e-28479.63Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F0996.9e-28479.47Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_031736005.10.095.96probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis sativus][more]
XP_008462723.10.095.48PREDICTED: probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis mel... [more]
XP_004142568.10.092.07probable serine/threonine-protein kinase WNK9 isoform X2 [Cucumis sativus] >KGN6... [more]
XP_031736006.10.090.53probable serine/threonine-protein kinase WNK9 isoform X3 [Cucumis sativus][more]
XP_038880702.10.084.94probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G04910.17.3e-17753.36with no lysine (K) kinase 1 [more]
AT3G04910.27.1e-16454.16with no lysine (K) kinase 1 [more]
AT5G28080.28.6e-16264.03Protein kinase superfamily protein [more]
AT3G22420.11.5e-15351.86with no lysine (K) kinase 2 [more]
AT3G22420.21.3e-14447.16with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 59..316
e-value: 5.1E-60
score: 215.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 61..316
e-value: 1.2E-49
score: 169.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 59..316
score: 39.297478
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 57..140
e-value: 3.4E-27
score: 96.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 142..321
e-value: 1.6E-49
score: 169.9
NoneNo IPR availableGENE3D3.10.20.90coord: 403..469
e-value: 4.0E-5
score: 25.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 565..586
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 60..642
NoneNo IPR availablePANTHERPTHR13902:SF144SERINE/THREONINE-PROTEIN KINASE WNK1coord: 60..642
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 59..316
e-value: 7.987E-168
score: 477.488
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 406..459
e-value: 3.8E-5
score: 23.6
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 183..195
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 62..317

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
IVF0004590IVF0004590gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0004590.2-five_prime_utrIVF0004590.2-five_prime_utr-chr02:17245464..17245626five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0004590.2-exonIVF0004590.2-exon-chr02:17245464..17245844exon
IVF0004590.2-exonIVF0004590.2-exon-chr02:17246141..17246368exon
IVF0004590.2-exonIVF0004590.2-exon-chr02:17246481..17246701exon
IVF0004590.2-exonIVF0004590.2-exon-chr02:17246926..17247083exon
IVF0004590.2-exonIVF0004590.2-exon-chr02:17247179..17247599exon
IVF0004590.2-exonIVF0004590.2-exon-chr02:17247702..17248690exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17245627..17245844CDS
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17246141..17246368CDS
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17246481..17246701CDS
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17246926..17247083CDS
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17247179..17247599CDS
IVF0004590.2-cdsIVF0004590.2-cds-chr02:17247702..17248384CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0004590.2-three_prime_utrIVF0004590.2-three_prime_utr-chr02:17248385..17248690three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
IVF0004590.2IVF0004590.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0035556 intracellular signal transduction
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity