IVF0000186.1 (mRNA) Melon (IVF77) v1

Overview
NameIVF0000186.1
TypemRNA
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionVacuolar-processing enzyme
Locationchr02: 16164743 .. 16168244 (+)
Sequence length1440
RNA-Seq ExpressionIVF0000186.1
SyntenyIVF0000186.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTACTTGTGATAGAAACCCAACGAAGCTATTAATCATTCTCATAACCTTTCTGTGTTTCAACAACATAATTGCTAAGAATGAGTGGATCAGTGATGCCACTACTGACCAAACTGGGAAGATATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGACACCAGGTACCCTTCTTGTATCATTTCAATTATACAAACTATACATAAAATTATTTGATGAAGTTACCATAATATAGCTGACAATTAAGAAGCATAAACAACTTTAGTTTGACTCAGAGTGTTCGTGTTGGACACATAAATACTCTTACACCTATTGGACATGTATCAAACATACACTTATCGGTGCAATAGACGTATTAGACACTAGTTAAGATTCAACATTTGTTAAACATGCATAAAACATTGTTTTACATCCGTATCCATCAAACTAATTAACTCTTTCTACCATAAAATGATATGTATTTTTTTAATAAATGTGTCTTCATGCTTGTCTCCTAGATTTAAAGAAAATGACACATCATCATGTCTTGTATTCATAACCGGCTTCTTAGATTTACAATATATATAATGCAACAAACTCATTGAATAATTATTCCAACCACTCGCTCTCCTACTAATAAATAGTAACTACCTTCTTCTTTTTTATATAAAAAACCAAAAACCTAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTACGATGATATTGCATTTCATCCGAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGAAATGATGTTTACCATGGAGTTCCCAAGGACTATACAGGAAAGCATGTGAATGCAGTCAACTTTTATGCTGCCATATTGGGAAACAAGTCTGCTCTGACTGGAGGAAGTGGTAAGGTTATTCATAGTGGCCCTCAGGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTCGGTAATAAGTTTATACTTACTACTACTTTTCGCCTCAACAAATTAAGGGTCTGTTTGGATTGATTCTCTAAATGCTTAGAAAATACAATTCAAACTGGTCAACCTTGAACTTCCTTAACTTCCTTATTAAATTTGGCTGTATTTTGTTCCACATGATTGTAGGAATGCCTGAAGGCGATTATATTTTTGTCAACGATCTAATAGATGTGCTGAAAAGGAAACATAAAGCCAAGAATTACAAAAGCATGGTACTAAAATATGGAATATAGATCATACAACAATAATTACGAAGTTCAAACTGTATATCAAAGTTACAATTAGTTAACATAAAAAATTCAGGTAATATATGTGGAAGCATGTGAGTCCGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAGCAGTCTCATCGTTCTCCCAAAGACTATAACACTTGCCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGGCAATATTTTATTTCAACACCTTTTATCTATATTAAAGAATTGATATAATTAAATTAACTCATACCTTTAAGCTTTTGAGTTCGGTTGTATTTTAACATAGTATGCATCCAAGTTCGAGTTCCTATATTCAGGCCCTTGTAACATCGGTTTCAAGTCTACATAGTCATTATCTAATCATCTTAAATAAATCTGTGTTGTGCCATAATGCGCAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATGCGGTAAGCTTCCTAATTCTTAAATGAACTCAATCTTCTTAAATCGCACCACTAATAAACATTTCCTTGTACATTTCCAAACTGCAGGTTCGAAGAAGAACTTTTGTTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTGACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATACTCTGTTCAGAGTCCTCAAACTATTTCATCGCTGTCAAAATTGTCATCAAATTCAGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGGTATGATTTATCCAACAACGTATATATTCAATTATGTTCCAATCTACTGCATAGTCTAAGTGTCTAGACTTTTGCTTTGAACAGTTTCAAGAAGCTCCATTTGGGTCTAGAGAAAAGATGGAGGCTAGAGAACAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAATAGTATAAATAGCATTGGCAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCACTGCTTCAAGACATTTGTAAGTCCTATTTTTCAACTCCAAACCTATTTTATACCATCAGAAACTTTGTTAATGCAATAATCTCTATAATACAGGTAAAAATCTATGAGAAACACTGCAACAGAATGTCCAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTGTCTCCAAACTTGCCTTGGTAAAACCTGAAGCGGGAATAATTTCTAATATTGTTAACCTGCCAATCTAGGCCCCCAGTGTTTATGTACGTATACAGAATTTGAAATGCAAATAATAAACTAATAATATTAATCTTGAACTGGAACCGTCGATCATTTCGAACTTTCTTTGCTAACACCCAAGCAAACTATCTACTCAAAAATCTTCTTTCAACCCCAGACAACAAATGCAATTGTAGCTCATTCAGAGGGAAGGAATCCGTATAAAGTTTGCTTTGGATCAGTATGGCAATGTGGGTGGTTTAATCAGACTTTATGAACAGAAAATTCTAAGTGGACGTAATTAAACAAATCATTAGATCTTTTTCAGCAAACTACGAGAAATTAAGCATAACAATTTCAACCACTGTGTTTTAAAACTAAATAAGAAAGATTAGCAATAATACGAACTTAAGGAATTTAGTCGGAGAAGAAACGGGGTGCTCCAAAGAGAACGACCTTTTTGCCGGTGGCGAGGGAGTGTACAGATTCTTGCGGGAGCTTATAGTCCTCGTCGAAGTAGTAGGCTGATGCCCCGTCCGGCAACGTATCCCCAACGGAAACCGGAGCCGGCTTTGATTTGGATGCATCGCCGGCGGCATCTAGTGGGAAGGGGATTTTGTACGGAAGGCCTCCAAATGAGATGTGACCGGGGATGCAAATTCTAACCAGTATTGTCTAATGATCAGTGTCTTGCGAAATTACTAAATACCCCCATCTGCCCGCAAGGCTAGTTTGCTGTGAAGTTCTCTCATTTCCATAAATTTCTCGTCCTTAGTTACTTGTTCTTGCTTAATCGTAATATCCAAATTCCTCACTTTATCTTCTTTTCGCTCTTCAATAATTTTTTGTTCCCCTTTCTCGTTCTAA

mRNA sequence

ATGACTACTTGTGATAGAAACCCAACGAAGCTATTAATCATTCTCATAACCTTTCTGTGTTTCAACAACATAATTGCTAAGAATGAGTGGATCAGTGATGCCACTACTGACCAAACTGGGAAGATATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGACACCAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTACGATGATATTGCATTTCATCCGAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGAAATGATCATGTGAATGCAGTCAACTTTTATGCTGCCATATTGGGAAACAAGTCTGCTCTGACTGGAGGAAGTGGTAAGGTTATTCATAGTGGCCCTCAGGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTCGGAATGCCTGAAGGCGATTATATTTTTGTCAACGATCTAATAGATGTGCTGAAAAGGAAACATAAAGCCAAGAATTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCCGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAGCAGTCTCATCGTTCTCCCAAAGACTATAACACTTGCCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATGCGGTTCGAAGAAGAACTTTTGTTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTGACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATACTCTGTTCAGAGTCCTCAAACTATTTCATCGCTGTCAAAATTGTCATCAAATTCAGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGTTTCAAGAAGCTCCATTTGGGTCTAGAGAAAAGATGGAGGCTAGAGAACAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAATAGTATAAATAGCATTGGCAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCACTGCTTCAAGACATTTGTAAAAATCTATGAGAAACACTGCAACAGAATGTCCAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTTTACTTGTTCTTGCTTAATCGTAATATCCAAATTCCTCACTTTATCTTCTTTTCGCTCTTCAATAATTTTTTGTTCCCCTTTCTCGTTCTAA

Coding sequence (CDS)

ATGACTACTTGTGATAGAAACCCAACGAAGCTATTAATCATTCTCATAACCTTTCTGTGTTTCAACAACATAATTGCTAAGAATGAGTGGATCAGTGATGCCACTACTGACCAAACTGGGAAGATATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGACACCAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTACGATGATATTGCATTTCATCCGAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGAAATGATCATGTGAATGCAGTCAACTTTTATGCTGCCATATTGGGAAACAAGTCTGCTCTGACTGGAGGAAGTGGTAAGGTTATTCATAGTGGCCCTCAGGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTCGGAATGCCTGAAGGCGATTATATTTTTGTCAACGATCTAATAGATGTGCTGAAAAGGAAACATAAAGCCAAGAATTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCCGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAGCAGTCTCATCGTTCTCCCAAAGACTATAACACTTGCCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATGCGGTTCGAAGAAGAACTTTTGTTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTGACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATACTCTGTTCAGAGTCCTCAAACTATTTCATCGCTGTCAAAATTGTCATCAAATTCAGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGTTTCAAGAAGCTCCATTTGGGTCTAGAGAAAAGATGGAGGCTAGAGAACAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAATAGTATAAATAGCATTGGCAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCACTGCTTCAAGACATTTGTAAAAATCTATGAGAAACACTGCAACAGAATGTCCAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTTTACTTGTTCTTGCTTAATCGTAATATCCAAATTCCTCACTTTATCTTCTTTTCGCTCTTCAATAATTTTTTGTTCCCCTTTCTCGTTCTAA

Protein sequence

MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCLIVISKFLTLSSFRSSIIFCSPFSF
Homology
BLAST of IVF0000186.1 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 482.6 bits (1241), Expect = 5.0e-135
Identity = 233/428 (54.44%), Postives = 303/428 (70.79%), Query Frame = 0

Query: 39  TGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGI 98
           +G  WA+L+AGS+GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 99  IINKPDG------------NDHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYS 158
           IIN P G             D VN  N +A ILG+K+A+ GGSGKV+ SGP DHIFI+YS
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 159 DHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKI 218
           DHG  G+LGMP   Y++ NDL DVLK+KH    YKS+V Y+EACESGS+FEG+LPE + I
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 219 YATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHA 278
           YATTASNA E SW TYCP +    P +Y TCLGDL+SVAWMEDS  H+L  ETL+QQY  
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYEL 295

Query: 279 VRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSS 338
           V+RRT      YGSHVM YG+  I  D LD ++G +P  DN+ ++       +SL K  S
Sbjct: 296 VKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA-----DANSL-KPPS 355

Query: 339 NSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQAR 398
              +QRDA L+H+  K+++AP GS  K EA++Q+ + +  R H DNS+  +GK+LFG +R
Sbjct: 356 RVTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISR 415

Query: 399 SSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQM 455
             E+L+ VR+ GQ LVDDW+C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M QM
Sbjct: 416 GPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQM 475

BLAST of IVF0000186.1 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 8.6e-135
Identity = 234/430 (54.42%), Postives = 307/430 (71.40%), Query Frame = 0

Query: 40  GKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGII 99
           G  WA+L AGS+GY NYRHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIAF+  NPR G+I
Sbjct: 47  GTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVI 106

Query: 100 INKPDGN------------DHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSD 159
           IN P+G+            + V A NFYAA+LG+KS LTGGSGKV++SGP DHIFI+YSD
Sbjct: 107 INSPNGDEVYKGVPKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSD 166

Query: 160 HGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIY 219
           HG  G+LG P G YI+ +DL +VLK+KH +  YK++V Y+EACESGS+FEG+LPE+I +Y
Sbjct: 167 HGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVY 226

Query: 220 ATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAV 279
           ATTASNA E SW TYCP +    P +Y+TCLGDL+SVAWMEDSD+H+L  ETL+QQY  V
Sbjct: 227 ATTASNADESSWGTYCPGEDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLV 286

Query: 280 RRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSN 339
           + RT      YGSHVM YG+  +  D L  +LG  P  +N  +  +     +SL   SS 
Sbjct: 287 KERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDPANENLTFVDE-----NSLWS-SSK 346

Query: 340 SVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARS 399
           +V+QRDA L+H+  KF++AP GS +K EAR+Q+ + +  R H D+S+  +GKLLFG  ++
Sbjct: 347 AVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKA 406

Query: 400 SEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMD 458
            E+L+ VR  G +LVDDW C KT V+ +E HC  +S YGMK+ R+ AN+CN GI   QM 
Sbjct: 407 PELLNAVRPAGSALVDDWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQMR 466

BLAST of IVF0000186.1 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 8.6e-135
Identity = 234/430 (54.42%), Postives = 304/430 (70.70%), Query Frame = 0

Query: 37  DQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D  G  WA+L+AGSNG+ NYRHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIAF+  NPRP
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 97  GIIINKPDGND------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIY 156
           G+IIN P G+D             V    F+A +LGNK+ALTGGSGKV+ SGP DHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 157 YSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP   YI+ ++LIDVLK+KH + NYKS+V Y+EACESGS+FEG+L E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 217 KIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQY 276
            IYATTASNA E SW TYCP +    P +Y+TCLGDL+S+AWMEDSD H+L  ETL+QQY
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNLRTETLHQQY 294

Query: 277 HAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKL 336
             V+ RT    + YGSHVM YG+  +  + L T+LG +P  DNY +  ++        + 
Sbjct: 295 ELVKTRT-ASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFVDENS------LRP 354

Query: 337 SSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQ 396
           +S +V+QRDA L+H+  K+++AP G+  K EA++Q  + +  R H D+SI  IGKLLFG 
Sbjct: 355 ASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFGI 414

Query: 397 ARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMH 455
            +  EIL+ VR  GQ LVDDW C K+ V+ +E HC  +S YGMK+ R++ANICN GI   
Sbjct: 415 EKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKE 474

BLAST of IVF0000186.1 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.5e-134
Identity = 234/430 (54.42%), Postives = 305/430 (70.93%), Query Frame = 0

Query: 37  DQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D  G  WA+L+AGSNGY NYRHQ+DVCHAYQ+L+KGG  +ENIIVFMYDDIA +  NPRP
Sbjct: 51  DFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRP 110

Query: 97  GIIINKPDGND------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIY 156
           G+IINKPDG+D             V+A NFYAA+LGNKSALTGGSGKV+ SGP DHIF+Y
Sbjct: 111 GVIINKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVY 170

Query: 157 YSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENI 216
           Y+DHG  G+LGMP G Y++ +DL +VLK+KH +  YKS+V Y+EACESGS+FEG+LP+++
Sbjct: 171 YTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDL 230

Query: 217 KIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQY 276
            IYATTASNA E SW  YCP      P +Y+TCLGDL+S+AWMEDS+ H+L  E+L QQY
Sbjct: 231 NIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQY 290

Query: 277 HAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKL 336
             V+ RT  +   YGSHVM YG+  +  + L  +LG +P  DN  +  ++  ++    + 
Sbjct: 291 KLVKNRTISEP--YGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETENSLK--LRT 350

Query: 337 SSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQ 396
            S +V+QRDA LIH+  KF++AP GS +K EA +Q+ + +  R+H DNS+  IG+LLFG 
Sbjct: 351 PSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGI 410

Query: 397 ARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMH 455
            + +E+L  VR  G  LVD+W C KT VK +E HC  +S YGMK+ R+ ANICNAGI   
Sbjct: 411 EKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNE 470

BLAST of IVF0000186.1 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 471.1 bits (1211), Expect = 1.5e-131
Identity = 228/434 (52.53%), Postives = 301/434 (69.35%), Query Frame = 0

Query: 37  DQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D     WA+L+AGS+GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 97  GIIINKPDG------------NDHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIY 156
           G+IIN P+G             D VN  N  A ILGNK+AL GGSGKV+ SGP DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 157 YSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP    ++ NDL DVLK+K+ +  YKS+V Y+EACESGS+FEG+LPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 217 KIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQY 276
            IYATTASNA E SW TYCP +    P +Y TCLGDL+SVAW+EDS+KH+L  ETL++QY
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 277 HAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY----SVQSPQTISS 336
             V++RT      YGSHVM +G+  +  + L  F+G +P  +N+ +    S++ P  ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 337 LSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKL 396
                    +QRDA L+H+ +K+Q+AP GS  K+EA++Q+ + +  R H DNSI  IG L
Sbjct: 339 ---------NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGIL 398

Query: 397 LFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAG 455
           LFG      +L+ VR  G+ LVDDW C K+ V+ +E+HC  +S YG+K+ R+IAN+CNAG
Sbjct: 399 LFG-LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAG 458

BLAST of IVF0000186.1 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.5e-254
Identity = 439/467 (94.00%), Postives = 440/467 (94.22%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
           RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
           RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF
Sbjct: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 456
           KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQA   +CL
Sbjct: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 455

BLAST of IVF0000186.1 vs. ExPASy TrEMBL
Match: A0A5D3DZP1 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00260 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 429/467 (91.86%), Postives = 430/467 (92.08%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
           RSPKDYNTCLGD          DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
           RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF
Sbjct: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 456
           KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQA   +CL
Sbjct: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 446

BLAST of IVF0000186.1 vs. ExPASy TrEMBL
Match: A0A1S3CJ43 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo OX=3656 GN=LOC103501567 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.2e-213
Identity = 369/385 (95.84%), Postives = 370/385 (96.10%), Query Frame = 0

Query: 83  MYDDIAFHPSNPRPGIIINKPDGND------------HVNAVNFYAAILGNKSALTGGSG 142
           MYDDIAFHPSNPRPGIIINKPDGND            HVNAVNFYAAILGNKSALTGGSG
Sbjct: 1   MYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHVNAVNFYAAILGNKSALTGGSG 60

Query: 143 KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 202
           KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC
Sbjct: 61  KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 120

Query: 203 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS 262
           ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS
Sbjct: 121 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS 180

Query: 263 DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY 322
           DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY
Sbjct: 181 DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY 240

Query: 323 SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA 382
           SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA
Sbjct: 241 SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA 300

Query: 383 DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT 442
           DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT
Sbjct: 301 DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT 360

Query: 443 RAIANICNAGITMHQMDQAFTCSCL 456
           RAIANICNAGITMHQMDQA   +CL
Sbjct: 361 RAIANICNAGITMHQMDQACLQTCL 385

BLAST of IVF0000186.1 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 3.1e-196
Identity = 345/467 (73.88%), Postives = 383/467 (82.01%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTT    PT LL+I + F  FN+IIAK EW+S A TDQ GK WALL+AGSNGYDNYRHQA
Sbjct: 1   MTTFATTPTTLLLIPLLF--FNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
           DVCHAYQIL+K GIPDENIIVFMYDDIAFHP NPRPGIIINKP G D             
Sbjct: 61  DVCHAYQILRK-GIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKD 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP DHIFIYY+DHGAAGMLGMP GDYI+ NDL+
Sbjct: 121 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKH+AK+YKSMVIYVEACESGSMFEG+LPEN+ IYATTASNATEDSWA YCP QS 
Sbjct: 181 DVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
                Y+TCLGDL+SVAWMEDSDKHD SKETLNQQYH VRRRT VDK GYGSHVMLYGN+
Sbjct: 241 TPHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNE 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
             GN+FL+T+ GN+ D +N + SV+S   ISSL    SN+VSQRDASL+HY YKFQ AP+
Sbjct: 301 SFGNNFLNTYFGNTSDDNNNESSVKSTHIISSL----SNAVSQRDASLLHYWYKFQNAPY 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEA EQLEDEI  RRHADNSIN IG LLFG+A+SS +L+  R  GQS VDDW+CF
Sbjct: 361 GSREKMEASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 456
           K +VKIYEKHC RMS YGMKYT+A+AN CNAGITM QMDQA   +C+
Sbjct: 421 KNYVKIYEKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCV 460

BLAST of IVF0000186.1 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 9.0e-196
Identity = 339/469 (72.28%), Postives = 392/469 (83.58%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           M TC    TK  ++LI  L F++IIAK E +  A+TDQ GK WA+L+AGS+GY+NYRHQA
Sbjct: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
           DVCHAYQIL+KGG+PDENIIVFMYDDIAF+PSNPRPG++INKPDG D            H
Sbjct: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VN++NFYA ILGN+SALTGGSGKV+ S   DHIFIYY+DHG+AG+LGMPEGDY++  DL+
Sbjct: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           +VLK+KH+AK+YKSMVIYVEACESGSM EG+LPENIKIYATTASNATE+SWATYCP Q  
Sbjct: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFP 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
             P DY+TCLGDL+S+AWMEDSDKHDLSKETL QQY AVRRRT VDK GYGSHVMLYGNK
Sbjct: 241 SPPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNK 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSK-LSSNSVSQRDASLIHYQYKFQEAP 360
            IGN+ LDT++G +PD  NY  SVQS  TI+  SK L SN+VSQRDASLIHY +KFQ+AP
Sbjct: 301 SIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420
           FGSREK EAR+QLEDEI  RRH D+SI  I KLLFGQA+SSE+L+NVR QGQSLVDDW C
Sbjct: 361 FGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCLI 457
           FK FVK YEKHC R+S YGMKYTRA+ANICNAGIT++QMDQA   +CL+
Sbjct: 421 FKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLV 468

BLAST of IVF0000186.1 vs. NCBI nr
Match: KAA0046547.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 439/467 (94.00%), Postives = 440/467 (94.22%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
           RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
           RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF
Sbjct: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 455
           KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQA   +CL
Sbjct: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 455

BLAST of IVF0000186.1 vs. NCBI nr
Match: TYK28710.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 855 bits (2210), Expect = 7.05e-312
Identity = 429/467 (91.86%), Postives = 430/467 (92.08%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
           RSPKDYNTCLGD          DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK
Sbjct: 241 RSPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
           RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF
Sbjct: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF
Sbjct: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 455
           KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQA   +CL
Sbjct: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 446

BLAST of IVF0000186.1 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 808 bits (2086), Expect = 1.26e-292
Identity = 398/468 (85.04%), Postives = 423/468 (90.38%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTTC+R+PTKLL+ILI F+ FNNIIAK EWISDAT +QTGK WALL+AGSNGY+NYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
           DVCHAYQILKKGGIPDENIIVFMYDDIAFHP+NPR GIIINKP G D             
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VNAVNFYAA+ GNKSALTGGSGKVIHSGP DHIFIYYSDHGAAGMLGMPE DY+FVNDLI
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           D LKRKHKAK+YKSMVIYVEACESGSMFEG+LPENIKIYATTASNATEDSWATYCPK+S 
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 241 RSP-KDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300
             P KDYNTCLGDL+SVAWMEDSDKHDLSKETLNQQYHAVRRRT VDKSG GSHVMLYGN
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMEDSDKHDLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGN 300

Query: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360
           +RIG DFL+TF+GNSPDY+NYK SVQS  TISS SKLSSN VSQRDASLI+YQYKFQ+AP
Sbjct: 301 RRIGTDFLNTFIGNSPDYNNYKSSVQSIHTISSPSKLSSNGVSQRDASLIYYQYKFQKAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420
            GSREKMEAREQLEDEI KRRHADNSIN+IGKLLFGQARSS++L+ VR QGQSLVDDWHC
Sbjct: 361 IGSREKMEAREQLEDEILKRRHADNSINNIGKLLFGQARSSKVLNKVREQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSCL 455
           FKTFVKIYEKHC RMSSYGMKYTRAIANICNAGITMHQMDQA   +CL
Sbjct: 421 FKTFVKIYEKHCKRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 468

BLAST of IVF0000186.1 vs. NCBI nr
Match: XP_008463400.2 (PREDICTED: vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo])

HSP 1 Score: 747 bits (1929), Expect = 4.51e-270
Identity = 369/385 (95.84%), Postives = 370/385 (96.10%), Query Frame = 0

Query: 83  MYDDIAFHPSNPRPGIIINKPDGND------------HVNAVNFYAAILGNKSALTGGSG 142
           MYDDIAFHPSNPRPGIIINKPDGND            HVNAVNFYAAILGNKSALTGGSG
Sbjct: 1   MYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHVNAVNFYAAILGNKSALTGGSG 60

Query: 143 KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 202
           KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC
Sbjct: 61  KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 120

Query: 203 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS 262
           ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS
Sbjct: 121 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDS 180

Query: 263 DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY 322
           DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY
Sbjct: 181 DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY 240

Query: 323 SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA 382
           SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA
Sbjct: 241 SVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHA 300

Query: 383 DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT 442
           DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT
Sbjct: 301 DNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYT 360

Query: 443 RAIANICNAGITMHQMDQAFTCSCL 455
           RAIANICNAGITMHQMDQA   +CL
Sbjct: 361 RAIANICNAGITMHQMDQACLQTCL 385

BLAST of IVF0000186.1 vs. NCBI nr
Match: XP_022987711.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima])

HSP 1 Score: 691 bits (1784), Expect = 8.77e-247
Identity = 346/466 (74.25%), Postives = 381/466 (81.76%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQA 60
           MTT    PT LL  LI  L FN+IIAK EW+S A TDQ GK WALL+AGSNGYDNYRHQA
Sbjct: 1   MTTFATTPTTLL--LIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------H 120
           DVCHAYQIL+KG IPDENIIVFMYDDIAFHP NPRPGIIINKP G D             
Sbjct: 61  DVCHAYQILRKG-IPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKD 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP DHIFIYY+DHGAAGMLGMP GDYI+ NDL+
Sbjct: 121 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 180

Query: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240
           DVLKRKH+AK+YKSMVIYVEACESGSMFEG+LPEN+ IYATTASNATEDSWA YCP QS 
Sbjct: 181 DVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 240

Query: 241 RSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNK 300
                Y+TCLGDL+SVAWMEDSDKHD SKETLNQQYH VRRRT VDK GYGSHVMLYGN+
Sbjct: 241 TPHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNE 300

Query: 301 RIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPF 360
             GN+FL+T+ GN+ D +N + SV+S   ISSLS    N+VSQRDASL+HY YKFQ AP+
Sbjct: 301 SFGNNFLNTYFGNTSDDNNNESSVKSTHIISSLS----NAVSQRDASLLHYWYKFQNAPY 360

Query: 361 GSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCF 420
           GSREKMEA EQLEDEI  RRHADNSIN IG LLFG+A+SS +L+  R  GQS VDDW+CF
Sbjct: 361 GSREKMEASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCF 420

Query: 421 KTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSC 454
           K +VKIYEKHC RMS YGMKYT+A+AN CNAGITM QMDQA   +C
Sbjct: 421 KNYVKIYEKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTC 459

BLAST of IVF0000186.1 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 482.6 bits (1241), Expect = 3.6e-136
Identity = 233/428 (54.44%), Postives = 303/428 (70.79%), Query Frame = 0

Query: 39  TGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGI 98
           +G  WA+L+AGS+GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 99  IINKPDG------------NDHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYS 158
           IIN P G             D VN  N +A ILG+K+A+ GGSGKV+ SGP DHIFI+YS
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 159 DHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKI 218
           DHG  G+LGMP   Y++ NDL DVLK+KH    YKS+V Y+EACESGS+FEG+LPE + I
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 219 YATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHA 278
           YATTASNA E SW TYCP +    P +Y TCLGDL+SVAWMEDS  H+L  ETL+QQY  
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYEL 295

Query: 279 VRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSS 338
           V+RRT      YGSHVM YG+  I  D LD ++G +P  DN+ ++       +SL K  S
Sbjct: 296 VKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFA-----DANSL-KPPS 355

Query: 339 NSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQAR 398
              +QRDA L+H+  K+++AP GS  K EA++Q+ + +  R H DNS+  +GK+LFG +R
Sbjct: 356 RVTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISR 415

Query: 399 SSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQM 455
             E+L+ VR+ GQ LVDDW+C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M QM
Sbjct: 416 GPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQM 475

BLAST of IVF0000186.1 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 471.1 bits (1211), Expect = 1.1e-132
Identity = 228/434 (52.53%), Postives = 301/434 (69.35%), Query Frame = 0

Query: 37  DQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D     WA+L+AGS+GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 97  GIIINKPDG------------NDHVNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIY 156
           G+IIN P+G             D VN  N  A ILGNK+AL GGSGKV+ SGP DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 157 YSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP    ++ NDL DVLK+K+ +  YKS+V Y+EACESGS+FEG+LPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 217 KIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQY 276
            IYATTASNA E SW TYCP +    P +Y TCLGDL+SVAW+EDS+KH+L  ETL++QY
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 277 HAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKY----SVQSPQTISS 336
             V++RT      YGSHVM +G+  +  + L  F+G +P  +N+ +    S++ P  ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 337 LSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINSIGKL 396
                    +QRDA L+H+ +K+Q+AP GS  K+EA++Q+ + +  R H DNSI  IG L
Sbjct: 339 ---------NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGIL 398

Query: 397 LFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAG 455
           LFG      +L+ VR  G+ LVDDW C K+ V+ +E+HC  +S YG+K+ R+IAN+CNAG
Sbjct: 399 LFG-LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAG 458

BLAST of IVF0000186.1 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 452.6 bits (1163), Expect = 3.9e-127
Identity = 225/462 (48.70%), Postives = 297/462 (64.29%), Query Frame = 0

Query: 10  KLLIILITFLCFNNIIAKNEWISDATTDQT-----GKIWALLIAGSNGYDNYRHQADVCH 69
           ++L+ L   L F+    K + ++D   + +     G  WA+L+AGSN Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------HVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIAF   NPRPG+IINKPDG D             VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP D+IFIYY+DHGA G++ MP GD +   D  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPK 249
           + HK K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E SW  YCP+     P 
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPS 248

Query: 250 DYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGN 309
           +  TCLGD FS++W+EDSD HD+SKETL QQYH V+RR   D     SHV  +G +++  
Sbjct: 249 EIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKMLK 308

Query: 310 DFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSRE 369
           D+L +++G +P+ DN+ ++      IS     +S  V+ RD  L++ Q K Q+AP GS E
Sbjct: 309 DYLSSYIGRNPENDNFTFTESFSSPIS-----NSGLVNPRDIPLLYLQRKIQKAPMGSLE 368

Query: 370 KMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFV 429
             EA+++L DE   R+  D SI  I +L   Q     +L + R  GQ LVDDW CFKT V
Sbjct: 369 SKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLV 428

Query: 430 KIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQAFTCSC 455
             ++ HC     YG+KYT A+ANICN G+ + Q   A   +C
Sbjct: 429 NSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQAC 464

BLAST of IVF0000186.1 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-126
Identity = 223/453 (49.23%), Postives = 294/453 (64.90%), Query Frame = 0

Query: 10  KLLIILITFLCFNNIIAKNEWISDATTDQT-----GKIWALLIAGSNGYDNYRHQADVCH 69
           ++L+ L   L F+    K + ++D   + +     G  WA+L+AGSN Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGND------------HVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIAF   NPRPG+IINKPDG D             VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP D+IFIYY+DHGA G++ MP GD +   D  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHRSPK 249
           + HK K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E SW  YCP+     P 
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPS 248

Query: 250 DYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGN 309
           +  TCLGD FS++W+EDSD HD+SKETL QQYH V+RR   D     SHV  +G +++  
Sbjct: 249 EIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKMLK 308

Query: 310 DFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSRE 369
           D+L +++G +P+ DN+ ++      IS     +S  V+ RD  L++ Q K Q+AP GS E
Sbjct: 309 DYLSSYIGRNPENDNFTFTESFSSPIS-----NSGLVNPRDIPLLYLQRKIQKAPMGSLE 368

Query: 370 KMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFV 429
             EA+++L DE   R+  D SI  I +L   Q     +L + R  GQ LVDDW CFKT V
Sbjct: 369 SKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLV 428

Query: 430 KIYEKHCNRMSSYGMKYTRAIANICNAGITMHQ 446
             ++ HC     YG+KYT A+ANICN G+ + Q
Sbjct: 429 NSFKNHCGATVHYGLKYTGALANICNMGVDVKQ 455

BLAST of IVF0000186.1 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 435.3 bits (1118), Expect = 6.5e-122
Identity = 219/442 (49.55%), Postives = 293/442 (66.29%), Query Frame = 0

Query: 32  SDATTDQTGKIWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHP 91
           +D   D  G  WA+L+AGS+GY NYRHQADVCHAYQIL+KGG+ +ENI+V MYDDIA HP
Sbjct: 41  ADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHP 100

Query: 92  SNPRPGIIINKPDGND------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPQD 151
            NPRPG +IN PDG+D             V A NFYA +LG++ A+ GGSGKVI S P D
Sbjct: 101 LNPRPGTLINHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPND 160

Query: 152 HIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEACESGSMFEGV 211
           HIF+YY+DHG  G+LGMP   +I+  D I+ LK+KH +  YK MVIYVEACESGS+FEG+
Sbjct: 161 HIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGI 220

Query: 212 LPENIKIYATTASNATEDSWATYCPKQSHRSPKDYNTCLGDLFSVAWMEDSDKHDLSKET 271
           +P+++ IY TTASNA E S+ TYCP  +   P +Y TCLGDL+SVAWMEDS+ H+L KET
Sbjct: 221 MPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKET 280

Query: 272 LNQQYHAVRRRT--FVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYKYSVQSPQT 331
           + QQYH V+ RT  +   SG GSHVM YGN  I ++ L            Y Y    P T
Sbjct: 281 IKQQYHTVKMRTSNYNTYSG-GSHVMEYGNNSIKSEKL------------YLYQGFDPAT 340

Query: 332 IS-SLSKLSSNS----VSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADN 391
           ++  L++L   S    V+QRDA L+   + ++ +  GSR+K +  ++L +    R+H D 
Sbjct: 341 VNLPLNELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDA 400

Query: 392 SINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKYTRA 451
           S+  I  +LFG   +  +L+ VR  G  LVDDW C K+ V+++E+HC  ++ YGMK+ RA
Sbjct: 401 SVELIATILFGP--TMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRA 460

Query: 452 IANICNAGITMHQMDQAFTCSC 455
            AN+CN G++   M++A T +C
Sbjct: 461 FANVCNNGVSKELMEEASTAAC 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q391195.0e-13554.44Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
O243258.6e-13554.42Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
P490438.6e-13554.42Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1[more]
P490442.5e-13454.42Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1[more]
P490471.5e-13152.53Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g... [more]
Match NameE-valueIdentityDescription
A0A5A7TXA72.5e-25494.00Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DZP11.2e-24591.86Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3CJ436.2e-21395.84vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1JF373.1e-19673.88vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1D4759.0e-19672.28vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ... [more]
Match NameE-valueIdentityDescription
KAA0046547.10.094.00vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
TYK28710.17.05e-31291.86vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
XP_038880044.11.26e-29285.04vacuolar-processing enzyme-like [Benincasa hispida][more]
XP_008463400.24.51e-27095.84PREDICTED: vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo][more]
XP_022987711.18.77e-24774.25vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G32940.13.6e-13654.44gamma vacuolar processing enzyme [more]
AT2G25940.11.1e-13252.53alpha-vacuolar processing enzyme [more]
AT3G20210.13.9e-12748.70delta vacuolar processing enzyme [more]
AT3G20210.21.5e-12649.23delta vacuolar processing enzyme [more]
AT1G62710.16.5e-12249.55beta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 43..67
score: 72.0
coord: 68..97
score: 61.45
coord: 180..196
score: 67.26
IPR001096Peptidase C13, legumainPIRSFPIRSF019663Legumaincoord: 5..469
e-value: 7.3E-187
score: 619.9
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 43..300
e-value: 8.9E-98
score: 326.9
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 28..455
IPR043577Asparaginyl endopeptidasePIRSFPIRSF500139AEcoord: 9..469
e-value: 1.7E-186
score: 618.8
NoneNo IPR availableGENE3D3.40.50.1460coord: 40..324
e-value: 4.2E-111
score: 373.0
NoneNo IPR availableGENE3D1.10.132.130coord: 331..461
e-value: 3.8E-33
score: 116.2
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 40..456
e-value: 4.7E-178
score: 590.7
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 28..455

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
IVF0000186IVF0000186gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0000186.1-exonIVF0000186.1-exon-chr02:16164743..16164919exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16165421..16165564exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16165601..16165742exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16165904..16165989exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16166081..16166280exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16166494..16166542exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16166627..16166854exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16166939..16167145exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16167223..16167337exon
IVF0000186.1-exonIVF0000186.1-exon-chr02:16168153..16168244exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16164743..16164919CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16165421..16165564CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16165601..16165742CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16165904..16165989CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16166081..16166280CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16166494..16166542CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16166627..16166854CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16166939..16167145CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16167223..16167337CDS
IVF0000186.1-cdsIVF0000186.1-cds-chr02:16168153..16168244CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
IVF0000186.1IVF0000186.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006624 vacuolar protein processing
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008233 peptidase activity