HG10021852.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10021852.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Phosphomethylpyrimidine synthase
Location	Chr05: 17568961 .. 17572029 (-)
Sequence length	1944
RNA-Seq Expression	HG10021852.1
Synteny	HG10021852.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAGTTCATGCTACCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCATACCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGACGTGTTTCCGGTGCATGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCGGGAAACCAGTACGGAGAAAGCCAAAAACAAGAAACATACAGTTGATCCTTCATCCCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGTGAGTGAAATCATTGGGGTATCATAGTTTCATTGTAATTGTTGCAAATCTAATAGATGATGATCATGCATTTACAATTATTGATGCATAATTTGTTATTGCAGGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAGAGTACCCTTTCGCCGGGTTCATTTATCTGGTGATGAACCGAACTTTGATAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGTATTGGTAAGATGATCAACTTTTTACTTTTTCTTGAAAGGACTCGTTTTTGCTATATTTGTGTTATATGCATAGGTTTTGTTCGTTATTTCTTAATTTTTCATGAATGTATATTTTTACTCAAAATATAACTTTTGTGAAAACTGTTTGGGTTAGATGTTGTTGCTGTAAATCTTTTTTTGTTTACTTTTATGTAGTGAACGGAGTTCTTCTATTTGGATATACATGTCTGCTATTTTAAGTTTGCAGCATAAGAGGGCTATTTTTTTCCCCTTAAATATTAGGTACCAACTAGTGTGAAAATGAAATCTTTTAACCGTGGGCCTATCTGACTCCATAGAGAAAAATAAATGGCGGCAGTACAATTGCTTTCTATGATGGAATGAAGTCCCTTTGATATTTTGTTACATTTTTTTTTAATCCAATATCTATTTATGATGCTTTCTGACTTCCATGATACAACAATGCAAATTAGCATACTTGTTACTGTCTTTCAATTTACACTGTTTCTGACAATCAACAATGTTATATAGGATTGCCTAAACTTCGGAAGGACTGGATTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTTTTGTGCCACTCGGGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGCGGGCGAGCAATCATCCCTTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCATCACATACACGAGACCCGTGAGTGGATATTGCGTAATTCATCCGTGCCAGTAGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGTGGGTCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGACATATCCCTGTCAATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAAAAAGACGTGCAGGTAGTGAATATAAGTCTCTCTTGCTTACATACATGCACGTGCTCACAATCACACCTATGTTAGTCATATGCATTTGAGAGCAAACCTTTTTACTCCTAGTTATATGCATTTGAAAGAACATCTCATAGTTGTAACTTTTAACTTTTGTAAGAAACACTCAAGGCTTACCTAATACAGTCAGTTTCATGCATTTCACAAACATTTGTGGCTGAATCATCAGGAATTTAGGAATCTATCGAGCAGTATCTTATGATTCATCACCCAATAATTTTTTTTTCGCTGGTTTGGTTGAGAGCTCCAATTTTCACTTAAATAGCAAATTCTTTTGGCACAATTAGTTTATTGGGAATGCACATTAGAAAATGGAACTATGATTTCCATCATTTTCAGTAACTAATAAAAGCAACAATTAGTGTCTGCTATTTTCATTTTTGGAAGTAGGAAATGTTATATTTTTCCTGTTACTAACTAGTGGCCACATACTTGAGTTTCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCTCCTTTTTACACTCTTGGTCCTTTGACAACAGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGCGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCACCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCATATAAGATAGCTGCTCATGCTGCTGATCTAGCCAAACGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCAAGGTTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGAGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGGCCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAAGAACATGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGACGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGA

mRNA sequence

ATGGCGTCAGTTCATGCTACCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCATACCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGACGTGTTTCCGGTGCATGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCGGGAAACCAGTACGGAGAAAGCCAAAAACAAGAAACATACAGTTGATCCTTCATCCCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAGAGTACCCTTTCGCCGGGTTCATTTATCTGGTGATGAACCGAACTTTGATAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGTATTGGATTGCCTAAACTTCGGAAGGACTGGATTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTTTTGTGCCACTCGGGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGCGGGCGAGCAATCATCCCTTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCATCACATACACGAGACCCGTGAGTGGATATTGCGTAATTCATCCGTGCCAGTAGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGTGGGTCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGACATATCCCTGTCAATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAAAAAGACGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCTCCTTTTTACACTCTTGGTCCTTTGACAACAGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGCGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCACCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCATATAAGATAGCTGCTCATGCTGCTGATCTAGCCAAACGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCAAGGTTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGAGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGGCCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAAGAACATGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGACGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGA

Coding sequence (CDS)

ATGGCGTCAGTTCATGCTACCGTAACACCAGCTGTTGGTAAGAGTGGGAACCGTTCATACCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGACGTGTTTCCGGTGCATGCAAGGACTTACACCCTTCATCCATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCGGGAAACCAGTACGGAGAAAGCCAAAAACAAGAAACATACAGTTGATCCTTCATCCCCTGATTTTCTGCCGCTTCCTTCATTTGAACAATGTTTCCCAAAAAGCACAAAAGAACACAAGGAAGTTGTTCACGAAGAAACTGGGCATGTGCTCAGAGTACCCTTTCGCCGGGTTCATTTATCTGGTGATGAACCGAACTTTGATAATTATGACACTAGTGGTCCTCAAAACATCAGCCCCCGTATTGGATTGCCTAAACTTCGGAAGGACTGGATTGATAGGAGGGACAAATTAGGCGCACCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTTTTGTGCCACTCGGGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGCGGGCGAGCAATCATCCCTTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCATCACATACACGAGACCCGTGAGTGGATATTGCGTAATTCATCCGTGCCAGTAGGAACTGTTCCCATATATCAGGCACTTGAAAAAGTGAATGGAATAGCTGAAAACCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACTATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGTGGGTCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTACGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGACATATCCCTGTCAATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCTTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAAAAAGACGTGCAGGTAATGAATGAAGGACCTGGACATATTCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCTCCTTTTTACACTCTTGGTCCTTTGACAACAGATGTAGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCTGCCAATATTGGCGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCACCTAGGATTGCCAAATCGAGATGATGTGAAGGCTGGAGTAATAGCATATAAGATAGCTGCTCATGCTGCTGATCTAGCCAAACGTCACCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCAAGGTTCGAGTTCCGATGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGAGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGGCCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGGAAGTATGCTGAAGAACATGGGTACGGGAGTGCAGAGGAAGCTCTGAAGCAAGGGATGGACGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGAGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGA

Protein sequence

MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLTFDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRRVHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSAEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR

Homology

[Close]

BLAST of HG10021852.1 vs. NCBI nr
Match: KAA0031806.1 (phosphomethylpyrimidine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 630/647 (97.37%), Postives = 639/647 (98.76%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+ ACKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of HG10021852.1 vs. NCBI nr
Match: XP_038894338.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida] >XP_038894339.1 phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 630/647 (97.37%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKSGNRS+ TK LNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSGNRSHATKPLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP  TSTEKAK+KKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVL+VPFRR
Sbjct: 61  FDPPGTSTEKAKSKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDW+DRR+KLG PRYTQMYYAK GIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKHGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICN YDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNLYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 647

[Close]

BLAST of HG10021852.1 vs. NCBI nr
Match: XP_038894337.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 630/647 (97.37%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKSGNRS+ TK LNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSGNRSHATKPLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP  TSTEKAK+KKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVH+ETGHVL+VPFRR
Sbjct: 61  FDPPGTSTEKAKSKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHKETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDW+DRR+KLG PRYTQMYYAK GIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWVDRREKLGTPRYTQMYYAKHGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICN YDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNLYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 647

[Close]

BLAST of HG10021852.1 vs. NCBI nr
Match: XP_008457389.1 (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 629/647 (97.22%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1    MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
            MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+  CKDLHPSSITLAPRATLT
Sbjct: 421  MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 480

Query: 61   FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
            FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 481  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 540

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 541  VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 600

Query: 181  MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 601  MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 660

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 661  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 720

Query: 301  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 721  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 780

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 781  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 840

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 841  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 900

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 901  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 960

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 961  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 1020

Query: 601  AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
            AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 1021 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 1067

[Close]

BLAST of HG10021852.1 vs. NCBI nr
Match: XP_008457386.1 (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis melo] >XP_008457388.1 PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 629/647 (97.22%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+  CKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot
Match: O82392 (Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THIC PE=1 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 596/646 (92.26%), Query Frame = 0


Query: 2   ASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  N++ LPGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRRV 121
           DP  T++E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVL+VPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+WIDRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTG HIHETREWILRNS+VPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK+HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot
Match: Q9KBJ4 (Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=thiC PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 2.1e-208
Identity = 372/584 (63.70%), Postives = 428/584 (73.29%), Query Frame = 0


Query: 90  SFEQCFPKSTKEHKEVVHEETGHVLRVPFRRVHL------SGDEPN--FDNYDTSGP--- 149
           S +  FP S K +      +    +RVPFR +        SG+  N     YD+SGP   
Sbjct: 12  SIQSSFPNSQKRYVTGSRSD----IRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTD 71

Query: 150 --QNISPRIGLPKLRKDWI----------------------------------------- 209
               ++   GLP +RK WI                                         
Sbjct: 72  ESYEVNIEKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHK 131

Query: 210 DRRDKLGAPRYTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLEL 269
             R K GA   TQM+YAK+GI+T EM F A RE + PEFVR E+ARGRAI+PSN NH E 
Sbjct: 132 PLRAKKGA-NVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPES 191

Query: 270 EPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGHHIHETREWI 329
           EPMI+GRNF VK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG +IH TREWI
Sbjct: 192 EPMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWI 251

Query: 330 LRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPL 389
           +RNS VPVGTVPIYQALEKVNG+AE+LTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPL
Sbjct: 252 IRNSPVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPL 311

Query: 390 TAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYD 449
           TAKR+TGIVSRGGSI A+WCL HHKENF Y H+++I +I   YDI+ S+GDGLRPGSI D
Sbjct: 312 TAKRVTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIAD 371

Query: 450 ANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLG 509
           AND AQFAEL T GELT+ AWE DVQVM EGPGH+PMH+I ENM KQL+ C EAPFYTLG
Sbjct: 372 ANDEAQFAELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLG 431

Query: 510 PLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHA 569
           PLTTD+APGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHA
Sbjct: 432 PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHA 491

Query: 570 ADLAKRHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSM 620
           ADLAK HP AQ  DDALSKARFEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSM
Sbjct: 492 ADLAKGHPGAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSM 551

[Close]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot
Match: Q5YNS3 (Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=thiC PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.1e-205
Identity = 359/546 (65.75%), Postives = 420/546 (76.92%), Query Frame = 0


Query: 98  STKEHKEVVHEETGHVLRVPFRRVHLSGDEPNFDNYDTSGP-----QNISPRIGLPKLRK 157
           S K ++EV        LR+P RR++L+  E  FD YDTSGP       I    GLPKLR 
Sbjct: 22  SVKHYREV------DGLRIPVRRINLTNGE-TFDVYDTSGPYTDENATIDLEAGLPKLRD 81

Query: 158 DWIDRRDKLGAPRYTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKNH 217
            W   +  +  PR TQ+ +A+ GI+T EM F A RE ++PE VR+EVA GRA+IP+N NH
Sbjct: 82  SW--DKPTVAGPR-TQLAWARAGIVTPEMRFIAAREGVEPELVRAEVAAGRAVIPANHNH 141

Query: 218 LELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGHHIHETR 277
            ELEP I+G+ FLVK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG +IHETR
Sbjct: 142 PELEPTIIGKKFLVKINANIGNSAVSSSIAEEVEKMVWATRWGADTIMDLSTGKNIHETR 201

Query: 278 EWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRY 337
           EWILRNS VPVGTVPIYQALEKVNG    LTWEI+R+T+IEQAEQGVDY T+HAGVLLRY
Sbjct: 202 EWILRNSPVPVGTVPIYQALEKVNGDPAALTWEIYRDTVIEQAEQGVDYMTVHAGVLLRY 261

Query: 338 IPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGS 397
           +PLTAKR+TGIVSRGGSI A WCLAHH+E+F Y H++++ +I  +YD++ S+GDGLRPGS
Sbjct: 262 VPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTHFEELCEILARYDVTFSLGDGLRPGS 321

Query: 398 IYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFY 457
           + DAND AQFAEL T GELT+ A    VQVM EGPGH+PMHKI EN++ + E C EAPFY
Sbjct: 322 VADANDEAQFAELRTLGELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEELCEEAPFY 381

Query: 458 TLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIA 517
           TLGPL TD+AP YDHITSAIGAA I   GTA+LCYVTPKEHLGLPNRDDVK GVI YKIA
Sbjct: 382 TLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPNRDDVKTGVITYKIA 441

Query: 518 AHAADLAKRHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHF 577
           AHAADLAK HPHAQ  DDALSKARFEFRW DQFALSLDP TA  +HDET+P+E AK AHF
Sbjct: 442 AHAADLAKGHPHAQQRDDALSKARFEFRWRDQFALSLDPDTAREYHDETMPAEPAKTAHF 501

Query: 578 CSMCGPKFCSMKITEDVRKYAEEHGYGSAEEALKQGMDAMSAEFLAAKKTISGEQHGETG 637
           CSMCGPKFCSM+I+ DVR+YAE +       A++ GM A SAEF             E+G
Sbjct: 502 CSMCGPKFCSMRISADVREYAEANNLTDV-AAIEAGMAAKSAEF------------AESG 544

Query: 638 GEIYLP 639
           G++YLP
Sbjct: 562 GKVYLP 544

[Close]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot
Match: A4VR38 (Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=thiC PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.1e-205
Identity = 370/600 (61.67%), Postives = 424/600 (70.67%), Query Frame = 0


Query: 93  QCFPKSTKEHKEVVHEETGHVLRVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 152
           Q FP+S K + +    +    +RVP R + L     +F          YDTSGP      
Sbjct: 21  QPFPRSQKIYVQGSRPD----IRVPMREISLDVTPTDFGGEINAPVTVYDTSGPYTDPNV 80

Query: 153 NISPRIGLPKLRKDWI-DRRD-------------------KLGAPRY------------- 212
            I  R GL  +R  WI DR D                   +L A R+             
Sbjct: 81  TIDVRKGLADVRSAWIEDRGDTEKLPGLTSEFGQRRLNDAELSAMRFAHVRNPRRAKAEH 140

Query: 213 --TQMYYAKQGIITEEMLFCATRE----------------------------KLDPEFVR 272
             +QM+YAK+GIIT EM + A RE                            ++ PEFVR
Sbjct: 141 NVSQMHYAKKGIITPEMEYVAIRENMKLAEAREAGLLVEQHAGQSFGAAIPKEITPEFVR 200

Query: 273 SEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGA 332
           SEVARGRAIIP+N NH ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WG+
Sbjct: 201 SEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGS 260

Query: 333 DTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAE 392
           DTVMDLSTG HIHETREWI+RNS VP+GTVPIYQALEKVNG+AE+LTWE+FR+TLIEQAE
Sbjct: 261 DTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAE 320

Query: 393 QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICN 452
           QGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I  
Sbjct: 321 QGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMK 380

Query: 453 QYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIP 512
            YD+S S+GDGLRPGSI DAND AQF EL T GELT+ AW+ DVQ M EGPGH+PMH I 
Sbjct: 381 AYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMHMIK 440

Query: 513 ENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGL 572
           ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGL
Sbjct: 441 ENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGL 500

Query: 573 PNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMS 617
           PN+DDVK G+I YKIAAHAADLAK HP AQ  D+ALSKARFEFRW DQF L LDP TA +
Sbjct: 501 PNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARA 560

[Close]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot
Match: A7GUZ5 (Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=thiC PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.8e-205
Identity = 365/563 (64.83%), Postives = 421/563 (74.78%), Query Frame = 0


Query: 103 KEVVHEETGHVLRVPFRRVHLSGDE--PN--FDNYDTSGP-----QNISPRIGLPKLRKD 162
           K+V  E +   ++VP R + LS     PN     YDTSGP       +    G+P  R++
Sbjct: 20  KKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRN 79

Query: 163 WI----------------------------------DR---RDKLGAPRYTQMYYAKQGI 222
           WI                                  DR   R K GA   TQM+YA++GI
Sbjct: 80  WIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGA-NVTQMHYARKGI 139

Query: 223 ITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSA 282
           IT EM + A RE ++PEFVR E+A GRAI+P+N NH E EPMI+GRNF VKVNANIGNSA
Sbjct: 140 ITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSA 199

Query: 283 VASSIEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVN 342
           V+SSI EEV K+ WAT WGADT+MDLSTG +IH TREWI+RN+ VPVGTVPIYQALEKV 
Sbjct: 200 VSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNAPVPVGTVPIYQALEKVQ 259

Query: 343 GIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCL 402
           GIAENLTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSI A+WCL
Sbjct: 260 GIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCL 319

Query: 403 AHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAW 462
            HHKENF Y H+++I +I  QYD+S S+GDGLRPGSI DAND AQFAEL T GELT+ AW
Sbjct: 320 YHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAW 379

Query: 463 EKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAAN 522
           + DVQVM EGPGH+PMH I ENM+K+++ C  APFYTLGPLTTD+APGYDHITSAIGAA 
Sbjct: 380 KHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAM 439

Query: 523 IGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKAR 582
           IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAADLAK H  AQ  DDALSKAR
Sbjct: 440 IGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGHKTAQQRDDALSKAR 499

Query: 583 FEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEH 620
           FEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMCGPKFCSMKI+ D+R+YA+E+
Sbjct: 500 FEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKEN 559

[Close]

BLAST of HG10021852.1 vs. ExPASy TrEMBL
Match: A0A5A7SPA0 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00490 PE=3 SV=1)

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 630/647 (97.37%), Postives = 639/647 (98.76%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+ ACKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of HG10021852.1 vs. ExPASy TrEMBL
Match: A0A1S3C5I7 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497093 PE=3 SV=1)

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 629/647 (97.22%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+  CKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of HG10021852.1 vs. ExPASy TrEMBL
Match: A0A5D3BDX2 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001330 PE=3 SV=1)

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 629/647 (97.22%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1   MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
           MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+  CKDLHPSSITLAPRATLT
Sbjct: 1   MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 60

Query: 61  FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
           FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 61  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
           VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300

Query: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
           WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 541 WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 600

Query: 601 AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

[Close]

BLAST of HG10021852.1 vs. ExPASy TrEMBL
Match: A0A1S3C5D6 (phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103497093 PE=3 SV=1)

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 629/647 (97.22%), Postives = 638/647 (98.61%), Query Frame = 0


Query: 1    MASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLT 60
            MASVHAT+TPAVGKS NRS PTKSLNTAFLPGFDVVGRV+  CKDLHPSSITLAPRATLT
Sbjct: 421  MASVHATITPAVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRATLT 480

Query: 61   FDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRR 120
            FDP ETSTEKAKN KHT+DPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVL+VPFRR
Sbjct: 481  FDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRR 540

Query: 121  VHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEE 180
            VHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLGAPR TQMYYAKQGIITEE
Sbjct: 541  VHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEE 600

Query: 181  MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 240
            MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 601  MLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASS 660

Query: 241  IEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 300
            IEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGIAE
Sbjct: 661  IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAE 720

Query: 301  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
            NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 721  NLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 780

Query: 361  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
            ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 781  ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 840

Query: 421  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
            QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 841  QVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 900

Query: 481  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFR 540
            GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFEFR
Sbjct: 901  GTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 960

Query: 541  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGS 600
            WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG+
Sbjct: 961  WMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGT 1020

Query: 601  AEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
            AEEAL+QGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 1021 AEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 1067

[Close]

BLAST of HG10021852.1 vs. ExPASy TrEMBL
Match: A0A0A0LXK6 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_1G528590 PE=3 SV=1)

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 629/649 (96.92%), Postives = 641/649 (98.77%), Query Frame = 0


Query: 1   MASVHATVTP--AVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRAT 60
           MASVHAT+TP  AVGKSGNRS PTKSLNTAFLPGFDVVGRV+ ACKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60

Query: 61  LTFDPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPF 120
           LTFDP ETSTEKAK++KHT+DPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVL+VPF
Sbjct: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNI+PRIGLPKLRKDW+DRRDKLG+PRYTQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180

Query: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTG HIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           GSAEEAL++GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 649

[Close]

BLAST of HG10021852.1 vs. TAIR 10
Match: AT2G29630.2 (thiaminC )

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 596/646 (92.26%), Query Frame = 0


Query: 2   ASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  N++ LPGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRRV 121
           DP  T++E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVL+VPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+WIDRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTG HIHETREWILRNS+VPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK+HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of HG10021852.1 vs. TAIR 10
Match: AT2G29630.1 (thiaminC )

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 596/646 (92.26%), Query Frame = 0


Query: 2   ASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  N++ LPGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRRV 121
           DP  T++E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVL+VPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+WIDRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTG HIHETREWILRNS+VPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK+HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]

BLAST of HG10021852.1 vs. TAIR 10
Match: AT2G29630.3 (thiaminC )

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 596/646 (92.26%), Query Frame = 0


Query: 2   ASVHATVTPAVGKSGNRSYPTKSLNTAFLPGFDVVGRVSGACKDLHPSSITLAPRATLTF 61
           ASVH T+   V  + N S   K  N++ LPGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPRETSTEKAKNKKHTVDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLRVPFRRV 121
           DP  T++E+AK +KHT+DPSSPDF P+PSFE+CFPKSTKEHKEVVHEE+GHVL+VPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPNFDNYDTSGPQNISPRIGLPKLRKDWIDRRDKLGAPRYTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  IGL KLRK+WIDRR+KLG PRYTQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGHHIHETREWILRNSSVPVGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTG HIHETREWILRNS+VPVGTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKRHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK+HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSA 601
           MDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYGSA
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALKQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

The following BLAST results are available for this feature:

BLAST of HG10021852.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0031806.1	0.0e+00	97.37	phosphomethylpyrimidine synthase [Cucumis melo var. makuwa]	[more]
XP_038894338.1	0.0e+00	97.37	phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Benincasa hispida] >...	[more]
XP_038894337.1	0.0e+00	97.37	phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_008457389.1	0.0e+00	97.22	PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis m...	[more]
XP_008457386.1	0.0e+00	97.22	PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis m...	[more]

BLAST of HG10021852.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82392	0.0e+00	86.07	Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9KBJ4	2.1e-208	63.70	Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / D...	[more]
Q5YNS3	2.1e-205	65.75	Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247...	[more]
A4VR38	2.1e-205	61.67	Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=37973...	[more]
A7GUZ5	2.8e-205	64.83	Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP...	[more]

BLAST of HG10021852.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SPA0	0.0e+00	97.37	Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3C5I7	0.0e+00	97.22	phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=36...	[more]
A0A5D3BDX2	0.0e+00	97.22	Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C5D6	0.0e+00	97.22	phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucumis melo OX=36...	[more]
A0A0A0LXK6	0.0e+00	96.92	Catalytic OS=Cucumis sativus OX=3659 GN=Csa_1G528590 PE=3 SV=1	[more]

BLAST of HG10021852.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29630.2	0.0e+00	86.07	thiaminC	[more]
AT2G29630.1	0.0e+00	86.07	thiaminC	[more]
AT2G29630.3	0.0e+00	86.07	thiaminC	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	6.10.250.620		coord: 527..559 e-value: 3.9E-18 score: 67.0
None	No IPR available	SFLD	SFLDG01114	phosphomethylpyrimidine_synth	coord: 165..621 e-value: 0.0 score: 837.1
None	No IPR available	SFLD	SFLDS00113	Radical_SAM_Phosphomethylpyri	coord: 165..623 e-value: 0.0 score: 856.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 63..79
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR30557:SF2	SUBFAMILY NOT NAMED	coord: 1..647
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	TIGRFAM	TIGR00190	TIGR00190	coord: 167..590 e-value: 4.1E-204 score: 676.1
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PFAM	PF01964	ThiC_Rad_SAM	coord: 167..587 e-value: 1.6E-197 score: 656.4
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	SFLD	SFLDF00407	phosphomethylpyrimidine_synth	coord: 167..587 e-value: 0.0 score: 657.3
IPR002817	Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/B	PANTHER	PTHR30557	THIAMINE BIOSYNTHESIS PROTEIN THIC	coord: 1..647
IPR038521	ThiC/Bza, core domain	GENE3D	3.20.20.540	Radical SAM ThiC family, central domain	coord: 209..523 e-value: 2.9E-168 score: 560.4
IPR037509	Phosphomethylpyrimidine synthase	HAMAP	MF_00089	ThiC	coord: 166..592 score: 94.740112

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10021852	HG10021852	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10021852.1-cds	HG10021852.1-cds-Chr05:17568961..17569641	CDS
HG10021852.1-cds	HG10021852.1-cds-Chr05:17570136..17570962	CDS
HG10021852.1-cds	HG10021852.1-cds-Chr05:17571490..17571617	CDS
HG10021852.1-cds	HG10021852.1-cds-Chr05:17571722..17572029	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10021852.1	HG10021852.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009526	plastid envelope
molecular_function	GO:0016830	carbon-carbon lyase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding