HG10015257.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015257.1 vs. NCBI nr
Match: XP_038892671.1 (WAT1-related protein At1g70260 [Benincasa hispida])

HSP 1 Score: 678.3 bits (1749), Expect = 3.7e-191
Identity = 356/372 (95.70%), Postives = 363/372 (97.58%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVKGE ILG VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY
Sbjct: 1   MEVKGERILGTVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYNHRMEYQQSLFSFPLL+RVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNHRMEYQQSLFSFPLLLRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISFILSILLGR KIEWKNPNFITKVVGT+ISVIGATCEELYLGPVVRQHP SSSTHLQF
Sbjct: 121 AISFILSILLGRTKIEWKNPNFITKVVGTVISVIGATCEELYLGPVVRQHP-SSSTHLQF 180

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNI+QLGV+KQYSQVMKVASFYS VGTLLS
Sbjct: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVIKQYSQVMKVASFYSSVGTLLS 240

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
           TIVAYFVVNDSSAWTIKS+FDL LIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI
Sbjct: 241 TIVAYFVVNDSSAWTIKSNFDLFLIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTV+WGQ+NEDRGVGEDKD DKNNDD LSS
Sbjct: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVLWGQINEDRGVGEDKDGDKNNDD-LSS 360

Query: 361 AKVPLLDEESVV 373
           AKVPLLDEES V
Sbjct: 361 AKVPLLDEESEV 370

BLAST of HG10015257.1 vs. NCBI nr
Match: XP_004135476.1 (WAT1-related protein At1g70260 [Cucumis sativus] >KAE8648724.1 hypothetical protein Csa_007846 [Cucumis sativus])

HSP 1 Score: 652.5 bits (1682), Expect = 2.2e-183
Identity = 335/369 (90.79%), Postives = 355/369 (96.21%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVKG GIL +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY
Sbjct: 3   MEVKGGGILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 62

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R+E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 63  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 122

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISF+LSILLG+ K+EWKNPNFITKVVGT+ISV+GATCEE+YLGP VRQHP SSSTHLQF
Sbjct: 123 AISFLLSILLGKTKMEWKNPNFITKVVGTVISVVGATCEEVYLGPTVRQHP-SSSTHLQF 182

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S
Sbjct: 183 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMS 242

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
            IVAYFVVNDSSAWT+KSSFDL LIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI
Sbjct: 243 AIVAYFVVNDSSAWTVKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 302

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFATFFG TFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +KNNDD LSS
Sbjct: 303 LFATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDRGVGKDKDINKNNDDSLSS 362

Query: 361 AKVPLLDEE 370
           AKVPLLD+E
Sbjct: 363 AKVPLLDDE 370

BLAST of HG10015257.1 vs. NCBI nr
Match: XP_008446238.1 (PREDICTED: WAT1-related protein At1g70260 [Cucumis melo])

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-181
Identity = 335/370 (90.54%), Postives = 355/370 (95.95%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVKG  IL +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVAS+ILLPY
Sbjct: 3   MEVKGGRILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPY 62

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R+E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 63  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 122

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           +ISF+LSILLG+ KIEW+NPNFITKVVGT+ISVIGATCEELYLGP VRQHP SSSTHLQF
Sbjct: 123 SISFLLSILLGKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHP-SSSTHLQF 182

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S
Sbjct: 183 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMS 242

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
            IVAYFVVNDSSAWTIKSSFDL LIIATGTFSGLIRNRVQIWCMQKKGP+YVPMFKPFGI
Sbjct: 243 AIVAYFVVNDSSAWTIKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGI 302

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKD-NDKNNDDLLS 360
           LFATFFG TFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +DKNNDD LS
Sbjct: 303 LFATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQVNEDRGVGKDKDVDDKNNDDSLS 362

Query: 361 SAKVPLLDEE 370
           SAKVPLLD+E
Sbjct: 363 SAKVPLLDDE 371

BLAST of HG10015257.1 vs. NCBI nr
Match: TYK15587.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 636.0 bits (1639), Expect = 2.1e-178
Identity = 328/360 (91.11%), Postives = 348/360 (96.67%), Query Frame = 0

Query: 11  VVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRM 70
           +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVAS+ILLPYSFIFHYN R+
Sbjct: 1   MVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPYSFIFHYNQRL 60

Query: 71  EYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILL 130
           E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP+ISF+LSILL
Sbjct: 61  EFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPSISFLLSILL 120

Query: 131 GRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQFKQKLLVFTST 190
           G+ KIEW+NPNFITKVVGT+ISVIGATCEELYLGP VRQHP SSSTHLQFKQKLLVFTST
Sbjct: 121 GKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHP-SSSTHLQFKQKLLVFTST 180

Query: 191 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVND 250
           TDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S IVAYFVVND
Sbjct: 181 TDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMSAIVAYFVVND 240

Query: 251 SSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTF 310
           SSAWTIKSSFDL LIIATGTFSGLIRNRVQIWCMQKKGP+YVPMFKPFGILFATFFG TF
Sbjct: 241 SSAWTIKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGILFATFFGATF 300

Query: 311 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKD-NDKNNDDLLSSAKVPLLDEE 370
           FGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +DKNNDD LSSAKVPLLD+E
Sbjct: 301 FGDTFHYGSVMAAFIAGMGYLTVMWGQVNEDRGVGKDKDVDDKNNDDSLSSAKVPLLDDE 359

BLAST of HG10015257.1 vs. NCBI nr
Match: XP_022957168.1 (WAT1-related protein At1g70260-like [Cucurbita moschata])

HSP 1 Score: 605.5 bits (1560), Expect = 3.0e-169
Identity = 324/372 (87.10%), Postives = 343/372 (92.20%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVK + I GVV PFLAMVS+EGCTIALTI+AKTAITYGM+TFVFVVYTNAVASI+LLPY
Sbjct: 1   MEVKKQ-IWGVV-PFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPY 60

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R E+QQSLFSFPL +RVFLLG  GIC+SQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISF+LSILLGR KI WK+P+FITKVVGT+ISVIGAT EELY+GP VRQHPSSSS HLQF
Sbjct: 121 AISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSS-HLQF 180

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           K KLLVFTSTTDRWI GGLLLAAA+LCVSIWNI+QLGV+KQY QVMKVASFYSLVGTLLS
Sbjct: 181 KHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLS 240

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
             V +FVVNDSSAWTIKSSFDL LIIATG FS LIRNRVQIWCMQ KGPYYVPMFKPFGI
Sbjct: 241 AAVGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGI 300

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED GVGEDKD DKNND  LSS
Sbjct: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKD-DKNNDH-LSS 360

Query: 361 AKVPLLDEESVV 373
           AK+PLLDEES V
Sbjct: 361 AKLPLLDEESKV 367

BLAST of HG10015257.1 vs. ExPASy Swiss-Prot
Match: F4I5D5 (WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.1e-100
Identity = 204/368 (55.43%), Postives = 261/368 (70.92%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           ++PF+AM  ME CTIALTI+AKTA+T GMS FVFVVYTNA  SI+LLP+SF+FH N R E
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             QS+FS+PLL+RVF LGFTGI + QNLAF+GL +SSPIVVCAMGL +P+ SF+LSI+LG
Sbjct: 70  --QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILG 129

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSS-STHLQFKQKLLVFTST 191
           R K++W+N +   K++GTI+S+ GA  EELY GP +R   S+S +  L+   KLLV+ + 
Sbjct: 130 RSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNL 189

Query: 192 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVND 251
            D W  G + LA A   VS++N+VQ G VK+Y  VMKVASFYS+VGT+   + + F+  D
Sbjct: 190 PDNWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERD 249

Query: 252 SSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTF 311
            SAW I+ +FDL LIIATGTF  +IR  V + C Q KGPYYVP+FKPFGI +AT FG +F
Sbjct: 250 LSAWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSF 309

Query: 312 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDL----LSSAKVPLL 371
           F ++ HYGSV+ A IAG+GY TV WGQ+ E        +  K+   +        KVPLL
Sbjct: 310 FVNSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDEDEYKVPLL 369

Query: 372 --DEESVV 373
              EES V
Sbjct: 370 INQEESPV 375

BLAST of HG10015257.1 vs. ExPASy Swiss-Prot
Match: Q9ZUI8 (WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-90
Identity = 190/360 (52.78%), Postives = 245/360 (68.06%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           ++PF+ M  ME CTIALTILAKTA+T GMS FVF+VYTNA+ S++LLPYSF FH +   +
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             +   + P L+R+FLLGFTG+ + QN+AFLGLSYSSPIVVCAMGL  PA SF+LS+ LG
Sbjct: 71  --EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALG 130

Query: 132 RI-KIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSS--STHLQFKQKLLVFT 191
           +   + W +     +V+GT+I   GA  E +YLGP +R  P SS  S  L      L F 
Sbjct: 131 KEGGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 192 STTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVV 251
             +D W  G LLLA ATL +SIWNI+QL  V++Y QVMKV S YSL GTL   I + F+ 
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFME 250

Query: 252 NDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGV 311
            D SAW +K + DL LIIATG F  +IR  VQ+ C + KGPYYVP+FKPFGIL+A+ FG 
Sbjct: 251 PDLSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGT 310

Query: 312 TFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSSAK--VPLL 367
           +FF ++ HYGSV+ A IAG GYL +MW Q+ +D     ++  +KN++  L S +   PLL
Sbjct: 311 SFFVNSLHYGSVLGAAIAGTGYLLIMWSQVQKD---DPNETVEKNDNHQLDSDEQTTPLL 365

BLAST of HG10015257.1 vs. ExPASy Swiss-Prot
Match: Q9FL08 (WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.7e-45
Identity = 116/330 (35.15%), Postives = 178/330 (53.94%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+PF AM ++E  T+    L K A   G+S +VFV Y+  V++++LLP S IF  + R+ 
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             +S    PL  ++FLLG  G  +SQ     G++YSSP +  A+  + PA +F L+++  
Sbjct: 78  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 137

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQFKQKLLVFTSTT 191
             ++  ++     K++G I+S+ GA    LY GP V    +S +T L         TS  
Sbjct: 138 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLA-SASFTTVLPTVTLHQQLTSIE 197

Query: 192 DRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVNDS 251
             WI GGLLLA+    +S+W I+Q  V++ Y + + V  FY+L  TL+S  V  F  ++ 
Sbjct: 198 SSWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNL 257

Query: 252 SAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTFF 311
           ++W +K    L  II +G F  L       W +  KGP Y+ +F+P  I  A   G  F 
Sbjct: 258 TSWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFL 317

Query: 312 GDTFHYGSVMAAFIAGMGYLTVMWGQMNED 342
           GD  H GSV+ + I  +G+ TV+WG+  ED
Sbjct: 318 GDALHLGSVIGSMILCIGFYTVIWGKARED 341

BLAST of HG10015257.1 vs. ExPASy Swiss-Prot
Match: Q94JU2 (WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.2e-45
Identity = 122/364 (33.52%), Postives = 189/364 (51.92%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+P  A+V ME   + L  L K A   GMS  VF+VY+  +A+++LLP  F    +  + 
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLPSLFCSFRSRTL- 69

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
                 +F +L ++ LLG  G C S  + + G++YSSP +  A+  + PA +F+L+++  
Sbjct: 70  ---PPMNFSILYKIVLLGIIG-CCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFR 129

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVV-RQHPSSSSTHLQFKQKLLVFTST 191
              + +K  + + K++GT++S+ GA    LY GPVV  + P S S   Q         ST
Sbjct: 130 MESVSFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------ST 189

Query: 192 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVV-N 251
              WI G   LA    CV +W IVQ  ++++Y     V  FYS+  +  + +V  F   N
Sbjct: 190 NPNWILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGN 249

Query: 252 DSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVT 311
           D  AW IK +  L+ I+ +G F   I N +  W ++ KGP +V MFKP  I  A   GV 
Sbjct: 250 DLGAWKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVI 309

Query: 312 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNE------DRGVGEDKDNDKNNDDLLSSAKV 368
           F  D+ + GS++ A +  +G+ TVMWG+  E      D     ++ N+ + D    S K 
Sbjct: 310 FLRDSLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKA 359

BLAST of HG10015257.1 vs. ExPASy Swiss-Prot
Match: F4KHA8 (WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.5e-43
Identity = 116/332 (34.94%), Postives = 179/332 (53.92%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+PF AMV++E  T+    L K A   G+S +VFV YT  VA+++LLP S IF  + R+ 
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATLVLLPLSLIFGRSKRLP 78

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             ++    P+   +FLL   G  +S  +   G+ YSSP +  A+  + PA +F L+++  
Sbjct: 79  SAKT----PVFFNIFLLALVGF-MSLIVGCKGIEYSSPTLASAISNLTPAFTFTLAVIFR 138

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSS--STHLQFKQKLLVFTS 191
             +I  ++     K++GTI+S+ GA    LY GP V    S +  S  +   Q L  F S
Sbjct: 139 MEQIVLRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQHLTSFDS 198

Query: 192 TTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVN 251
           +   WI GGLLLA   L VS+W I+Q  V++ Y + + V   Y+L  TL+S  V  F   
Sbjct: 199 S---WIIGGLLLATQYLLVSVWYILQTRVMELYPEEITVVFLYNLCATLISAPVCLFAEK 258

Query: 252 DSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVT 311
           D +++ +K    L  ++ +G       + +  W +  KGP Y+ +FKP  I+ A   GV 
Sbjct: 259 DLNSFILKPGVSLASVMYSGGLVSSFGSVIHTWGLHLKGPVYISLFKPLSIVIAVAMGVM 318

Query: 312 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNED 342
           F GD  + GSV+ + I  +G+ TV+WG+  ED
Sbjct: 319 FLGDALYLGSVIGSLILSLGFYTVIWGKARED 342

BLAST of HG10015257.1 vs. ExPASy TrEMBL
Match: A0A0A0KQA2 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G602730 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.0e-183
Identity = 335/369 (90.79%), Postives = 355/369 (96.21%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVKG GIL +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY
Sbjct: 1   MEVKGGGILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R+E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISF+LSILLG+ K+EWKNPNFITKVVGT+ISV+GATCEE+YLGP VRQHP SSSTHLQF
Sbjct: 121 AISFLLSILLGKTKMEWKNPNFITKVVGTVISVVGATCEEVYLGPTVRQHP-SSSTHLQF 180

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S
Sbjct: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMS 240

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
            IVAYFVVNDSSAWT+KSSFDL LIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI
Sbjct: 241 AIVAYFVVNDSSAWTVKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFATFFG TFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +KNNDD LSS
Sbjct: 301 LFATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDRGVGKDKDINKNNDDSLSS 360

Query: 361 AKVPLLDEE 370
           AKVPLLD+E
Sbjct: 361 AKVPLLDDE 368

BLAST of HG10015257.1 vs. ExPASy TrEMBL
Match: A0A1S3BEK3 (WAT1-related protein At1g70260 OS=Cucumis melo OX=3656 GN=LOC103489028 PE=4 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 7.5e-182
Identity = 335/370 (90.54%), Postives = 355/370 (95.95%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVKG  IL +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVAS+ILLPY
Sbjct: 3   MEVKGGRILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPY 62

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R+E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 63  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 122

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           +ISF+LSILLG+ KIEW+NPNFITKVVGT+ISVIGATCEELYLGP VRQHP SSSTHLQF
Sbjct: 123 SISFLLSILLGKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHP-SSSTHLQF 182

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S
Sbjct: 183 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMS 242

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
            IVAYFVVNDSSAWTIKSSFDL LIIATGTFSGLIRNRVQIWCMQKKGP+YVPMFKPFGI
Sbjct: 243 AIVAYFVVNDSSAWTIKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGI 302

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKD-NDKNNDDLLS 360
           LFATFFG TFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +DKNNDD LS
Sbjct: 303 LFATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQVNEDRGVGKDKDVDDKNNDDSLS 362

Query: 361 SAKVPLLDEE 370
           SAKVPLLD+E
Sbjct: 363 SAKVPLLDDE 371

BLAST of HG10015257.1 vs. ExPASy TrEMBL
Match: A0A5D3CUM5 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00810 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.0e-178
Identity = 328/360 (91.11%), Postives = 348/360 (96.67%), Query Frame = 0

Query: 11  VVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRM 70
           +V+PFLAMV+MEGCTIALTILAKTAITYGMSTFVFVVYTNAVAS+ILLPYSFIFHYN R+
Sbjct: 1   MVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPYSFIFHYNQRL 60

Query: 71  EYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILL 130
           E+QQSLFSFPLL+RVFLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP+ISF+LSILL
Sbjct: 61  EFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPSISFLLSILL 120

Query: 131 GRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQFKQKLLVFTST 190
           G+ KIEW+NPNFITKVVGT+ISVIGATCEELYLGP VRQHP SSSTHLQFKQKLLVFTST
Sbjct: 121 GKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHP-SSSTHLQFKQKLLVFTST 180

Query: 191 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVND 250
           TDRWIFGGLLLAAATLCVSIWNI+QLGVVKQYSQVMKV SFYS+VGT +S IVAYFVVND
Sbjct: 181 TDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMSAIVAYFVVND 240

Query: 251 SSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTF 310
           SSAWTIKSSFDL LIIATGTFSGLIRNRVQIWCMQKKGP+YVPMFKPFGILFATFFG TF
Sbjct: 241 SSAWTIKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGILFATFFGATF 300

Query: 311 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKD-NDKNNDDLLSSAKVPLLDEE 370
           FGDTFHYGSVMAAFIAGMGYLTVMWGQ+NEDRGVG+DKD +DKNNDD LSSAKVPLLD+E
Sbjct: 301 FGDTFHYGSVMAAFIAGMGYLTVMWGQVNEDRGVGKDKDVDDKNNDDSLSSAKVPLLDDE 359

BLAST of HG10015257.1 vs. ExPASy TrEMBL
Match: A0A6J1GZG2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.5e-169
Identity = 324/372 (87.10%), Postives = 343/372 (92.20%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVK + I GVV PFLAMVS+EGCTIALTI+AKTAITYGM+TFVFVVYTNAVASI+LLPY
Sbjct: 1   MEVKKQ-IWGVV-PFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPY 60

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFHYN R E+QQSLFSFPL +RVFLLG  GIC+SQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISF+LSILLGR KI WK+P+FITKVVGT+ISVIGAT EELY+GP VRQHPSSSS HLQF
Sbjct: 121 AISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSS-HLQF 180

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           K KLLVFTSTTDRWI GGLLLAAA+LCVSIWNI+QLGV+KQY QVMKVASFYSLVGTLLS
Sbjct: 181 KHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLS 240

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
             V +FVVNDSSAWTIKSSFDL LIIATG FS LIRNRVQIWCMQ KGPYYVPMFKPFGI
Sbjct: 241 AAVGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGI 300

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED GVGEDKD DKNND  LSS
Sbjct: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKD-DKNNDH-LSS 360

Query: 361 AKVPLLDEESVV 373
           AK+PLLDEES V
Sbjct: 361 AKLPLLDEESKV 367

BLAST of HG10015257.1 vs. ExPASy TrEMBL
Match: A0A6J1IKD8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.6e-168
Identity = 321/372 (86.29%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 1   MEVKGEGILGVVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60
           MEVK + I GVV PFLAMVS+EGCTIALTI+AKTAITYGM+TFVFVVYTNAVASI+LLPY
Sbjct: 1   MEVKKQ-IWGVV-PFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPY 60

Query: 61  SFIFHYNHRMEYQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           S IFHYN R E+QQSLFSFPL +RVFLLG  GIC+SQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SLIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLGRIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQF 180
           AISF+LSI+LGR KI WK+P+FITKVVGT+ISVIGAT EELY+GP VRQHPSSSS HLQF
Sbjct: 121 AISFVLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSS-HLQF 180

Query: 181 KQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLS 240
           K KLLVFTSTTDRWI GGLLLAAA+LCVSIWNI+QLGV+KQY QVMKVASFYSLVGTLLS
Sbjct: 181 KHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQLGVIKQYPQVMKVASFYSLVGTLLS 240

Query: 241 TIVAYFVVNDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGI 300
             V +FVVNDSSAWTIKSSFDL LIIATG FS LIRNRVQIWCMQ KGPYYVPMFKPFGI
Sbjct: 241 AAVGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGI 300

Query: 301 LFATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSS 360
           LFAT FGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQ+NED GVGEDKD DKNND  LSS
Sbjct: 301 LFATLFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKD-DKNNDH-LSS 360

Query: 361 AKVPLLDEESVV 373
           AK+PLLDEES V
Sbjct: 361 AKLPLLDEESKV 367

BLAST of HG10015257.1 vs. TAIR 10
Match: AT1G70260.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 366.7 bits (940), Expect = 2.2e-101
Identity = 204/368 (55.43%), Postives = 261/368 (70.92%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           ++PF+AM  ME CTIALTI+AKTA+T GMS FVFVVYTNA  SI+LLP+SF+FH N R E
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             QS+FS+PLL+RVF LGFTGI + QNLAF+GL +SSPIVVCAMGL +P+ SF+LSI+LG
Sbjct: 70  --QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILG 129

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSS-STHLQFKQKLLVFTST 191
           R K++W+N +   K++GTI+S+ GA  EELY GP +R   S+S +  L+   KLLV+ + 
Sbjct: 130 RSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNL 189

Query: 192 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVND 251
            D W  G + LA A   VS++N+VQ G VK+Y  VMKVASFYS+VGT+   + + F+  D
Sbjct: 190 PDNWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERD 249

Query: 252 SSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTF 311
            SAW I+ +FDL LIIATGTF  +IR  V + C Q KGPYYVP+FKPFGI +AT FG +F
Sbjct: 250 LSAWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSF 309

Query: 312 FGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDL----LSSAKVPLL 371
           F ++ HYGSV+ A IAG+GY TV WGQ+ E        +  K+   +        KVPLL
Sbjct: 310 FVNSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDEDEYKVPLL 369

Query: 372 --DEESVV 373
              EES V
Sbjct: 370 INQEESPV 375

BLAST of HG10015257.1 vs. TAIR 10
Match: AT1G60050.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 335.1 bits (858), Expect = 7.1e-92
Identity = 190/360 (52.78%), Postives = 245/360 (68.06%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           ++PF+ M  ME CTIALTILAKTA+T GMS FVF+VYTNA+ S++LLPYSF FH +   +
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             +   + P L+R+FLLGFTG+ + QN+AFLGLSYSSPIVVCAMGL  PA SF+LS+ LG
Sbjct: 71  --EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALG 130

Query: 132 RI-KIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSS--STHLQFKQKLLVFT 191
           +   + W +     +V+GT+I   GA  E +YLGP +R  P SS  S  L      L F 
Sbjct: 131 KEGGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 192 STTDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVV 251
             +D W  G LLLA ATL +SIWNI+QL  V++Y QVMKV S YSL GTL   I + F+ 
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFME 250

Query: 252 NDSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGV 311
            D SAW +K + DL LIIATG F  +IR  VQ+ C + KGPYYVP+FKPFGIL+A+ FG 
Sbjct: 251 PDLSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGT 310

Query: 312 TFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDRGVGEDKDNDKNNDDLLSSAK--VPLL 367
           +FF ++ HYGSV+ A IAG GYL +MW Q+ +D     ++  +KN++  L S +   PLL
Sbjct: 311 SFFVNSLHYGSVLGAAIAGTGYLLIMWSQVQKD---DPNETVEKNDNHQLDSDEQTTPLL 365

BLAST of HG10015257.1 vs. TAIR 10
Match: AT5G40240.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-46
Identity = 116/330 (35.15%), Postives = 178/330 (53.94%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+PF AM ++E  T+    L K A   G+S +VFV Y+  V++++LLP S IF  + R+ 
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             +S    PL  ++FLLG  G  +SQ     G++YSSP +  A+  + PA +F L+++  
Sbjct: 78  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 137

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQFKQKLLVFTSTT 191
             ++  ++     K++G I+S+ GA    LY GP V    +S +T L         TS  
Sbjct: 138 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLA-SASFTTVLPTVTLHQQLTSIE 197

Query: 192 DRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVNDS 251
             WI GGLLLA+    +S+W I+Q  V++ Y + + V  FY+L  TL+S  V  F  ++ 
Sbjct: 198 SSWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNL 257

Query: 252 SAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTFF 311
           ++W +K    L  II +G F  L       W +  KGP Y+ +F+P  I  A   G  F 
Sbjct: 258 TSWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFL 317

Query: 312 GDTFHYGSVMAAFIAGMGYLTVMWGQMNED 342
           GD  H GSV+ + I  +G+ TV+WG+  ED
Sbjct: 318 GDALHLGSVIGSMILCIGFYTVIWGKARED 341

BLAST of HG10015257.1 vs. TAIR 10
Match: AT5G40240.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-46
Identity = 116/330 (35.15%), Postives = 178/330 (53.94%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+PF AM ++E  T+    L K A   G+S +VFV Y+  V++++LLP S IF  + R+ 
Sbjct: 32  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 91

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
             +S    PL  ++FLLG  G  +SQ     G++YSSP +  A+  + PA +F L+++  
Sbjct: 92  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 151

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVVRQHPSSSSTHLQFKQKLLVFTSTT 191
             ++  ++     K++G I+S+ GA    LY GP V    +S +T L         TS  
Sbjct: 152 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLA-SASFTTVLPTVTLHQQLTSIE 211

Query: 192 DRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVVNDS 251
             WI GGLLLA+    +S+W I+Q  V++ Y + + V  FY+L  TL+S  V  F  ++ 
Sbjct: 212 SSWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNL 271

Query: 252 SAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVTFF 311
           ++W +K    L  II +G F  L       W +  KGP Y+ +F+P  I  A   G  F 
Sbjct: 272 TSWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFL 331

Query: 312 GDTFHYGSVMAAFIAGMGYLTVMWGQMNED 342
           GD  H GSV+ + I  +G+ TV+WG+  ED
Sbjct: 332 GDALHLGSVIGSMILCIGFYTVIWGKARED 355

BLAST of HG10015257.1 vs. TAIR 10
Match: AT3G28050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-46
Identity = 122/364 (33.52%), Postives = 189/364 (51.92%), Query Frame = 0

Query: 12  VIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPYSFIFHYNHRME 71
           V+P  A+V ME   + L  L K A   GMS  VF+VY+  +A+++LLP  F    +  + 
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLPSLFCSFRSRTL- 69

Query: 72  YQQSLFSFPLLIRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLG 131
                 +F +L ++ LLG  G C S  + + G++YSSP +  A+  + PA +F+L+++  
Sbjct: 70  ---PPMNFSILYKIVLLGIIG-CCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFR 129

Query: 132 RIKIEWKNPNFITKVVGTIISVIGATCEELYLGPVV-RQHPSSSSTHLQFKQKLLVFTST 191
              + +K  + + K++GT++S+ GA    LY GPVV  + P S S   Q         ST
Sbjct: 130 MESVSFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------ST 189

Query: 192 TDRWIFGGLLLAAATLCVSIWNIVQLGVVKQYSQVMKVASFYSLVGTLLSTIVAYFVV-N 251
              WI G   LA    CV +W IVQ  ++++Y     V  FYS+  +  + +V  F   N
Sbjct: 190 NPNWILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGN 249

Query: 252 DSSAWTIKSSFDLILIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGILFATFFGVT 311
           D  AW IK +  L+ I+ +G F   I N +  W ++ KGP +V MFKP  I  A   GV 
Sbjct: 250 DLGAWKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVI 309

Query: 312 FFGDTFHYGSVMAAFIAGMGYLTVMWGQMNE------DRGVGEDKDNDKNNDDLLSSAKV 368
           F  D+ + GS++ A +  +G+ TVMWG+  E      D     ++ N+ + D    S K 
Sbjct: 310 FLRDSLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKA 359