HG10014875.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10014875.1
TypemRNA
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionTropinone reductase-like protein
LocationChr02: 21223719 .. 21226028 (+)
Sequence length822
RNA-Seq ExpressionHG10014875.1
SyntenyHG10014875.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGCCGGCGACAGTTCCGGAAAATCCCGATGGTCTCTCAAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGGTTTGCTAAATTTTCTAACTCTGTTTACTCCAAAACCTTCTTGAATTTCGATTCTCTTCTGCTCTGTGTACTATTATCTCTCTGTTTTATAGTCGCGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCGTCGGTCCAAACCTGCTCCCGGAATGAATCGGAGCTCAATCAGTGCTTGAAGGAATGGGAGGGCAAGGGATTTGTGGTCAGTGGATCGGTTTGCGATGCTTCTTTAAGAACACAAAGAGAGGGGCTGATACAGGAAGTGGCGTCTGTTTTCAGTGGCACTCTCAATATTCTGGTCAGTAATACTATGCTTCTGCCCATTTTAATTTTGGAAATAACAGTGTTGAAATTGGGCGTTGTTTGTTGCATGGCTTTGGGTTGGGAATTTGAAGCTCTTATATCACAATTTTTGGTCATTTTGCTTTTAGCTTTACTTTCAAATTAGTGGTTGTAAAACTTCAAGTTTCATCATTATTGTTTATATGCTGTCTGGTCTCTGGCATTAACCAAAATGAATGTTATTGGGATAGAATTAGGAATATTATTAGAATATTAATGGCATATTAATGATGAGTTAGGGAGGTTAATATGATTACAAATAGAATGAGCGGGAAATGATGTAGGTAGGCAATTCTTTAGATGGGTGAGGCTTTAGTGTATATACTCAAGAGATGGAAGGTTCCAATTCCAAGTACCTTGAGTAATTTTGTTCTAAAAGTATCCAATTAGCTCCTAGTTATATATTATGTAGGAAATGGACAGAATACACATGTACTTTTTTGCTTGACAATCAAATGTTGTAGGATTAGACCAAAAAAAAAAAAAGACAGATACAATTTATTGATATATTTATGGTTGAGCTCCAAGAAGGATAAAAATGCCTGCAAAGTCGGACAAACTTTAGTCGCTCTTGTATTTTTAATGAAAATAACGAATGAAACCACTGTAAAGAGATCTAAATTCTAAAGTAATGTCATTGTAGGCAGGTGCTATGATTTTTTCATGCTTGACATCCACAATAGACATTTTTTAGTAAAATATTTAGTGAATTGCAATGGAACCATTGAAACAAGAAACTAGAAAGCCTTCTATTGGCCTCTTGTAGTTCAAAGTTTAAAACCCGCCATTTTTTGAAGCAATAATGTCTCGTTGTTGGCTCGATAATCAATCACATGACCCTTAGGAAGTTGCCTTCTAGGAACTTTGACGGTTTCTTTGTGTAGGTAAACAATGTGGGAACAAACATAAGGAAGCCAAGTATTGAGTACAGTTTGGAAGATGTTTCGGCATTGATGACAACAAATTTTGAATCTGCATATCATTTATCCCAACTTTCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCGTTTCTTCGGTTGCTGGTCTTGTGAGCATTGGAAGTGGATCCATCTATGCTGCAACTAAATGTGAGTTGCTATTGTAAAACTTAGTTTCTTTTATTTGGTATGTTCTAAGAAGCTAAAACTGTTCCCCTTTTTTATTTTCTACTCAGCTGCAATAAATCAACTGACCAGAAATCTTGCTTGTGAATGGGCCAAGGATAATATCAGGATCAATGGTGTTGCACCCTGGTATATAAATACCCCTCTGGTAGAAAAGGTAATATTTTTTCAACCACCTGCAAAATAATGTGTCTTCTTTTTATGTCTCTTCTAAAAAACAAATGTTGAGAATGGAAGCGATATTGGAAAAAATTGTCTTCTTTCCTTTTAATCTTATCCATCTTTTTCTGAAATCTCTATGAATGATAAATTGAATCACTTAGGCAACATTGATTTGGTAACTATTGAATGTACAAGGACTAAAACAGTATTTAAACCCACATTTTGATCTTGAAATTTTTTTATAACAACATCTAGTGACAGAACACTCTGCAATTCATATATCTTTTGAGTTTAAGAGTGTCTATCTTGATTGCATGTGTTTTCACCCATAAACCTCTTGAATGTTTAACTAATAACAAGGGTTATTACCATCATAGTTGCTGAATAATAAGACGTTGGTGGACAAGATAGTTTCTCGAACACCACTCGGGCGCACAGGGGAATCGAACGAAGTGTCATCGTTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACTGGACAGATTGTTTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGGCTAGACAAGTTGTGA

mRNA sequence

ATGGCGGAGGCCGGCGACAGTTCCGGAAAATCCCGATGGTCTCTCAAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGTCGCGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCGTCGGTCCAAACCTGCTCCCGGAATGAATCGGAGCTCAATCAGTGCTTGAAGGAATGGGAGGGCAAGGGATTTGTGGTCAGTGGATCGGTTTGCGATGCTTCTTTAAGAACACAAAGAGAGGGGCTGATACAGGAAGTGGCGTCTGTTTTCAGTGGCACTCTCAATATTCTGGTAAACAATGTGGGAACAAACATAAGGAAGCCAAGTATTGAGTACAGTTTGGAAGATGTTTCGGCATTGATGACAACAAATTTTGAATCTGCATATCATTTATCCCAACTTTCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCGTTTCTTCGGTTGCTGGTCTTGTGAGCATTGGAAGTGGATCCATCTATGCTGCAACTAAATCTGCAATAAATCAACTGACCAGAAATCTTGCTTGTGAATGGGCCAAGGATAATATCAGGATCAATGGTGTTGCACCCTGGTATATAAATACCCCTCTGGTAGAAAAGTTGCTGAATAATAAGACGTTGGTGGACAAGATAGTTTCTCGAACACCACTCGGGCGCACAGGGGAATCGAACGAAGTGTCATCGTTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACTGGACAGATTGTTTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGGCTAGACAAGTTGTGA

Coding sequence (CDS)

ATGGCGGAGGCCGGCGACAGTTCCGGAAAATCCCGATGGTCTCTCAAAGGCTTCACCGCGCTCGTCACCGGCGGCACTCGCGGAATCGGTCGCGCTGTGGTGGAGGAACTAGCCGGTTTAGGCGCGTCGGTCCAAACCTGCTCCCGGAATGAATCGGAGCTCAATCAGTGCTTGAAGGAATGGGAGGGCAAGGGATTTGTGGTCAGTGGATCGGTTTGCGATGCTTCTTTAAGAACACAAAGAGAGGGGCTGATACAGGAAGTGGCGTCTGTTTTCAGTGGCACTCTCAATATTCTGGTAAACAATGTGGGAACAAACATAAGGAAGCCAAGTATTGAGTACAGTTTGGAAGATGTTTCGGCATTGATGACAACAAATTTTGAATCTGCATATCATTTATCCCAACTTTCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGCATTGTGTTCGTTTCTTCGGTTGCTGGTCTTGTGAGCATTGGAAGTGGATCCATCTATGCTGCAACTAAATCTGCAATAAATCAACTGACCAGAAATCTTGCTTGTGAATGGGCCAAGGATAATATCAGGATCAATGGTGTTGCACCCTGGTATATAAATACCCCTCTGGTAGAAAAGTTGCTGAATAATAAGACGTTGGTGGACAAGATAGTTTCTCGAACACCACTCGGGCGCACAGGGGAATCGAACGAAGTGTCATCGTTGGTGGCATTCCTTTGCTTGCCTGCTGCATCTTACATTACTGGACAGATTGTTTCTGTTGATGGAGGATTCACTGCAAATGGATTTGAACCTGGTATGAGGCTAGACAAGTTGTGA

Protein sequence

MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASYITGQIVSVDGGFTANGFEPGMRLDKL
Homology
BLAST of HG10014875.1 vs. NCBI nr
Match: XP_038889667.1 (tropinone reductase homolog At5g06060-like [Benincasa hispida])

HSP 1 Score: 500.4 bits (1287), Expect = 1.0e-137
Identity = 255/273 (93.41%), Postives = 264/273 (96.70%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           M EAGDSSGKSRWSLKG TALVTGGTRGIG A+VEELAGLGASV TCSRNES+LN+CLKE
Sbjct: 1   MTEAGDSSGKSRWSLKGCTALVTGGTRGIGHALVEELAGLGASVHTCSRNESDLNRCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WEGKGFVVSGSVCDAS RTQRE LIQEVASVF GTLNILVNNVGTNIRKPS EYSLE+VS
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREELIQEVASVFCGTLNILVNNVGTNIRKPSTEYSLEEVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
           ALMTTNFESAYHLSQLSHPLLKASGNGSIVF++SVAGLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFITSVAGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIRINGVAPWYINTPLVEKLL+NKTLVDKIVSRTPLGR GES+EVSSLVAFL
Sbjct: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLSNKTLVDKIVSRTPLGRIGESDEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIISVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. NCBI nr
Match: XP_008461702.1 (PREDICTED: tropinone reductase homolog At5g06060-like isoform X1 [Cucumis melo] >KAA0050360.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK03573.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 480.7 bits (1236), Expect = 8.3e-132
Identity = 244/273 (89.38%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEA DS+G  RWSLKGFTALVTGGTRGIG AVVEELAGLGASV TCSRN+S+LNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WE KGFVV+GSVCDAS RTQRE LIQEVAS F+GTLNILVNN GTNIRKP+ EYSLEDVS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESAYHLSQLSHPLLKASGNGSIVF+SSV GLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR+NGVAPWYINTPLVEKLLNNKTLVD IVSRTPL R GES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. NCBI nr
Match: XP_022976820.1 (tropinone reductase homolog At5g06060 [Cucurbita maxima])

HSP 1 Score: 479.2 bits (1232), Expect = 2.4e-131
Identity = 243/273 (89.01%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEAG SSG SRWSLKGFTALVTGGTRGIGRAVVEELAGLGASV TCSRNE ELNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WEGKGFVVSGSVCDAS RTQRE L+QEVAS F+GTLNILVNNVGTNIRKPSIEYSLE+ S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFES +HLSQL+HPLLKASGNGSIVFVSSV GLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR N VAPWY NT LVEKLL+NKT VDK+VSRTPLGR GESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFEPGMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of HG10014875.1 vs. NCBI nr
Match: XP_023547195.1 (tropinone reductase homolog At5g06060 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.6 bits (1228), Expect = 7.0e-131
Identity = 242/273 (88.64%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEAG SSG +RWSLKGFTALVTGGTRGIGRAVVEELAGLGASV TCSRNE ELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WEGKGFVVSGSVCDAS RTQRE L+QEVAS F+GTLNILVNNVGTNIRKPSIEYSLE+ S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFES +HLSQL+HPLLKASGNGSIVFVSSV GLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 ILMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR N VAPWY NTPLVEKLL+NKT VDK+VSRTPLGR GESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFE GMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLDSL 273

BLAST of HG10014875.1 vs. NCBI nr
Match: XP_004150641.1 (tropinone reductase homolog At5g06060 [Cucumis sativus] >KGN50576.1 hypothetical protein Csa_021491 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 7.0e-131
Identity = 242/273 (88.64%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEA  S+G SRWSL+GFTALVTGGTRGIG AVVEELAGLGASV TC+RNES+LNQCLKE
Sbjct: 1   MAEASGSAGNSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WE KG+VV+GSVCDAS RTQRE LIQEVAS F+GTLNILVNNVGTNIRKPS EYSLE+VS
Sbjct: 61  WEAKGYVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESA+HLSQLSHPLLKASGNGSIVF+SSV GLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           L CEWAKDNIR+N VAPWYINTPLVEKL+ NKTLVD IVSRTPLGR GES EVSSLVAFL
Sbjct: 181 LTCEWAKDNIRVNCVAPWYINTPLVEKLMKNKTLVDNIVSRTPLGRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.0e-105
Identity = 189/258 (73.26%), Postives = 214/258 (82.95%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFVVSGS 71
           RWSL G TALVTGGTRGIGRAVVEELA  GA V TCSRN+ ELN CL +W+  G VVSGS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNFESAY 131
           VCDAS+R QRE LIQE +S FSG LNIL+NNVGTN+RKP++EYS E+ + +M+TN ESA+
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAKDNIR 191
           HLSQ++HPLLKASG GSIVF+SSVAGLV + SGSIY ATK A+NQLTRNLACEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 INGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASYITGQ 251
            N VAPWYI T LVE LL  K  V+ +VSRTPLGR GE  EVSSLVAFLCLPA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IVSVDGGFTANGFEPGMR 270
           ++SVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of HG10014875.1 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 9.3e-94
Identity = 169/259 (65.25%), Postives = 209/259 (80.69%), Query Frame = 0

Query: 8   SGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFV 67
           + K RWSL G +ALVTGGTRGIGRA+VEELAGLGA V TC+RNE EL  CL +W   GF 
Sbjct: 61  TSKERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFR 120

Query: 68  VSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNF 127
           V+GSVCD S R+QRE L++ V+SVF G L+ILVNNVGTNIRKP +E++  + S LM+TNF
Sbjct: 121 VAGSVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNF 180

Query: 128 ESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAK 187
           ES +HL QL++PLL+ S  GS+VF+SSV+G VS+ + S+ ++TK AINQLTR+LACEWAK
Sbjct: 181 ESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAK 240

Query: 188 DNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASY 247
           DNIRIN VAPWYI T +VE++L+NK  ++++ S TPLGR GE  EVSS VAFLCLPA+SY
Sbjct: 241 DNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSY 300

Query: 248 ITGQIVSVDGGFTANGFEP 267
           ITGQI+ VDGG + NGF P
Sbjct: 301 ITGQILCVDGGMSINGFFP 319

BLAST of HG10014875.1 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-90
Identity = 171/265 (64.53%), Postives = 210/265 (79.25%), Query Frame = 0

Query: 1   MAEAGDSS-GKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLK 60
           MA+AG++S  KSRWSL+G TALVTGG++G+G AVVEELA LGA V TC+R+E++L + L+
Sbjct: 1   MAKAGENSRDKSRWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLR 60

Query: 61  EWEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDV 120
           EW+ KGF V+ SVCD S R QRE L++ V+SVF G LNILVNN GT I KPS EY+ ED 
Sbjct: 61  EWQAKGFEVTTSVCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDY 120

Query: 121 SALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTR 180
           S LM TN ESA+HLSQ++HPLLKASG+GSIVF+SSVAGLV  G+ SIY A+K A+NQL R
Sbjct: 121 SFLMATNLESAFHLSQIAHPLLKASGSGSIVFMSSVAGLVHTGA-SIYGASKGAMNQLGR 180

Query: 181 NLACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAF 240
           +LACEWA DNIR+N V PW I TPL   + +++ L   +  +TP+GR GE+NEVSSLVAF
Sbjct: 181 SLACEWASDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAF 240

Query: 241 LCLPAASYITGQIVSVDGGFTANGF 265
           LC PAASYITGQ + VDGG + NGF
Sbjct: 241 LCFPAASYITGQTICVDGGASVNGF 264

BLAST of HG10014875.1 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.1e-90
Identity = 168/265 (63.40%), Postives = 207/265 (78.11%), Query Frame = 0

Query: 1   MAEAGDS-SGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLK 60
           MA+A ++   K RWSL G TALVTGG++G+G AVVEELA LGA V TC+RNE++L +C++
Sbjct: 1   MAKAEENLRDKCRWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVR 60

Query: 61  EWEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDV 120
           EW+ KGF V+ SVCD S R QRE L++ VAS+F G LNILVNN GT I KP+ EY+ +D 
Sbjct: 61  EWQAKGFEVTTSVCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDY 120

Query: 121 SALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTR 180
           S LM TN +SA+HLSQL+HPLLKASG+GSIV +SS AG+V I  GSIY ATK A+NQL +
Sbjct: 121 SFLMATNLDSAFHLSQLAHPLLKASGSGSIVLMSSTAGVVHINVGSIYGATKGAMNQLAK 180

Query: 181 NLACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAF 240
           NLACEWA+DNIR+N V PW+I TPL    LN++ L  ++  +TP+GR G +NEVSSLVAF
Sbjct: 181 NLACEWARDNIRVNSVCPWFIATPL---YLNDEELKKEVERKTPMGRVGNANEVSSLVAF 240

Query: 241 LCLPAASYITGQIVSVDGGFTANGF 265
           LC PAASYITGQ + VDGGFT N F
Sbjct: 241 LCFPAASYITGQTICVDGGFTVNCF 262

BLAST of HG10014875.1 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 3.7e-90
Identity = 162/253 (64.03%), Postives = 202/253 (79.84%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFVVSGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LGA V TC+R+E++L + L+EW+ KGF V+ S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNFESAY 131
           +CD SLR QRE L++ V+S+F G LNILVNNVGT + KP+ EY+ E+ S LM TN +SA+
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAKDNIR 191
           H+SQL+HPLLKASG+GSIV +SS+AG+V +G GSIY ATK A+NQL RNLACEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 INGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASYITGQ 251
            N + PW I TPL+  LL+ + +  +   RTP+GR GE+NEVS LVAFLCLPAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IVSVDGGFTANGF 265
           ++ VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

BLAST of HG10014875.1 vs. ExPASy TrEMBL
Match: A0A5D3BV45 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00720 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.0e-132
Identity = 244/273 (89.38%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEA DS+G  RWSLKGFTALVTGGTRGIG AVVEELAGLGASV TCSRN+S+LNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WE KGFVV+GSVCDAS RTQRE LIQEVAS F+GTLNILVNN GTNIRKP+ EYSLEDVS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESAYHLSQLSHPLLKASGNGSIVF+SSV GLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR+NGVAPWYINTPLVEKLLNNKTLVD IVSRTPL R GES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. ExPASy TrEMBL
Match: A0A1S3CF70 (tropinone reductase homolog At5g06060-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500241 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.0e-132
Identity = 244/273 (89.38%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEA DS+G  RWSLKGFTALVTGGTRGIG AVVEELAGLGASV TCSRN+S+LNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WE KGFVV+GSVCDAS RTQRE LIQEVAS F+GTLNILVNN GTNIRKP+ EYSLEDVS
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESAYHLSQLSHPLLKASGNGSIVF+SSV GLVS+GSGSIYAATKSAINQLT+ 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR+NGVAPWYINTPLVEKLLNNKTLVD IVSRTPL R GES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. ExPASy TrEMBL
Match: A0A6J1IKI1 (tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC111477082 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.2e-131
Identity = 243/273 (89.01%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEAG SSG SRWSLKGFTALVTGGTRGIGRAVVEELAGLGASV TCSRNE ELNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WEGKGFVVSGSVCDAS RTQRE L+QEVAS F+GTLNILVNNVGTNIRKPSIEYSLE+ S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFES +HLSQL+HPLLKASGNGSIVFVSSV GLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR N VAPWY NT LVEKLL+NKT VDK+VSRTPLGR GESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFEPGMRLD L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of HG10014875.1 vs. ExPASy TrEMBL
Match: A0A0A0KS92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.4e-131
Identity = 242/273 (88.64%), Postives = 255/273 (93.41%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEA  S+G SRWSL+GFTALVTGGTRGIG AVVEELAGLGASV TC+RNES+LNQCLKE
Sbjct: 1   MAEASGSAGNSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WE KG+VV+GSVCDAS RTQRE LIQEVAS F+GTLNILVNNVGTNIRKPS EYSLE+VS
Sbjct: 61  WEAKGYVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFESA+HLSQLSHPLLKASGNGSIVF+SSV GLVSIGSGSIYAATKSAINQLTRN
Sbjct: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           L CEWAKDNIR+N VAPWYINTPLVEKL+ NKTLVD IVSRTPLGR GES EVSSLVAFL
Sbjct: 181 LTCEWAKDNIRVNCVAPWYINTPLVEKLMKNKTLVDNIVSRTPLGRIGESKEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           CLPAASYITGQI+SVDGGFTANGFEPGMRLDKL
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of HG10014875.1 vs. ExPASy TrEMBL
Match: A0A6J1GWZ5 (tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458319 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.2e-130
Identity = 240/273 (87.91%), Postives = 254/273 (93.04%), Query Frame = 0

Query: 1   MAEAGDSSGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKE 60
           MAEAG SSG +RWSLKGFTALVTGGTRGIGRAVVEELAGLG SV TCSRNE ELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVS 120
           WEGKGFVVSGSVCDAS RTQRE L+QEVAS F+GTLNILVNNVGTNIRKPSIEYSLE+ S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 ALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRN 180
            LMTTNFES +HLSQL+HPLLKASGNGSIVFVSSV GLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFL 240
           LACEWAKDNIR N VAPWY NTPLVEKLL+NKT VDK+VSRTPLGR GESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CLPAASYITGQIVSVDGGFTANGFEPGMRLDKL 274
           C+PAASYITGQI+SVDGGFTANGFE GMRL+ L
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 273

BLAST of HG10014875.1 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 2.9e-106
Identity = 189/258 (73.26%), Postives = 214/258 (82.95%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFVVSGS 71
           RWSL G TALVTGGTRGIGRAVVEELA  GA V TCSRN+ ELN CL +W+  G VVSGS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNFESAY 131
           VCDAS+R QRE LIQE +S FSG LNIL+NNVGTN+RKP++EYS E+ + +M+TN ESA+
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAKDNIR 191
           HLSQ++HPLLKASG GSIVF+SSVAGLV + SGSIY ATK A+NQLTRNLACEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 INGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASYITGQ 251
            N VAPWYI T LVE LL  K  V+ +VSRTPLGR GE  EVSSLVAFLCLPA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IVSVDGGFTANGFEPGMR 270
           ++SVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of HG10014875.1 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 6.6e-95
Identity = 169/259 (65.25%), Postives = 209/259 (80.69%), Query Frame = 0

Query: 8   SGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFV 67
           + K RWSL G +ALVTGGTRGIGRA+VEELAGLGA V TC+RNE EL  CL +W   GF 
Sbjct: 61  TSKERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFR 120

Query: 68  VSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNF 127
           V+GSVCD S R+QRE L++ V+SVF G L+ILVNNVGTNIRKP +E++  + S LM+TNF
Sbjct: 121 VAGSVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNF 180

Query: 128 ESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAK 187
           ES +HL QL++PLL+ S  GS+VF+SSV+G VS+ + S+ ++TK AINQLTR+LACEWAK
Sbjct: 181 ESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAK 240

Query: 188 DNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASY 247
           DNIRIN VAPWYI T +VE++L+NK  ++++ S TPLGR GE  EVSS VAFLCLPA+SY
Sbjct: 241 DNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSY 300

Query: 248 ITGQIVSVDGGFTANGFEP 267
           ITGQI+ VDGG + NGF P
Sbjct: 301 ITGQILCVDGGMSINGFFP 319

BLAST of HG10014875.1 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 8.9e-92
Identity = 171/265 (64.53%), Postives = 210/265 (79.25%), Query Frame = 0

Query: 1   MAEAGDSS-GKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLK 60
           MA+AG++S  KSRWSL+G TALVTGG++G+G AVVEELA LGA V TC+R+E++L + L+
Sbjct: 1   MAKAGENSRDKSRWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLR 60

Query: 61  EWEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDV 120
           EW+ KGF V+ SVCD S R QRE L++ V+SVF G LNILVNN GT I KPS EY+ ED 
Sbjct: 61  EWQAKGFEVTTSVCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDY 120

Query: 121 SALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTR 180
           S LM TN ESA+HLSQ++HPLLKASG+GSIVF+SSVAGLV  G+ SIY A+K A+NQL R
Sbjct: 121 SFLMATNLESAFHLSQIAHPLLKASGSGSIVFMSSVAGLVHTGA-SIYGASKGAMNQLGR 180

Query: 181 NLACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAF 240
           +LACEWA DNIR+N V PW I TPL   + +++ L   +  +TP+GR GE+NEVSSLVAF
Sbjct: 181 SLACEWASDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAF 240

Query: 241 LCLPAASYITGQIVSVDGGFTANGF 265
           LC PAASYITGQ + VDGG + NGF
Sbjct: 241 LCFPAASYITGQTICVDGGASVNGF 264

BLAST of HG10014875.1 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 2.6e-91
Identity = 162/253 (64.03%), Postives = 202/253 (79.84%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLKEWEGKGFVVSGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LGA V TC+R+E++L + L+EW+ KGF V+ S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDVSALMTTNFESAY 131
           +CD SLR QRE L++ V+S+F G LNILVNNVGT + KP+ EY+ E+ S LM TN +SA+
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTRNLACEWAKDNIR 191
           H+SQL+HPLLKASG+GSIV +SS+AG+V +G GSIY ATK A+NQL RNLACEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 INGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAFLCLPAASYITGQ 251
            N + PW I TPL+  LL+ + +  +   RTP+GR GE+NEVS LVAFLCLPAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IVSVDGGFTANGF 265
           ++ VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

BLAST of HG10014875.1 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 9.9e-91
Identity = 165/265 (62.26%), Postives = 207/265 (78.11%), Query Frame = 0

Query: 1   MAEAGDS-SGKSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVQTCSRNESELNQCLK 60
           MA+ G+S   K RWSL G TALVTGG++GIG AVVEELA LGA + TC+R+E++L + L+
Sbjct: 1   MAKTGESLRDKPRWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLR 60

Query: 61  EWEGKGFVVSGSVCDASLRTQREGLIQEVASVFSGTLNILVNNVGTNIRKPSIEYSLEDV 120
           +W+ KGF V+ SVCD S R +RE L++ V+++F G LNILVNNVGT I KP+++++ ED 
Sbjct: 61  KWQAKGFQVTTSVCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDF 120

Query: 121 SALMTTNFESAYHLSQLSHPLLKASGNGSIVFVSSVAGLVSIGSGSIYAATKSAINQLTR 180
           S  M TN ESA+HLSQL+HPLLKASG+GSIV +SSV+G+V +   SIY  +K A+NQL R
Sbjct: 121 SFTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGR 180

Query: 181 NLACEWAKDNIRINGVAPWYINTPLVEKLLNNKTLVDKIVSRTPLGRTGESNEVSSLVAF 240
           NLACEWA DNIR N V PW+I TPLV + L+N+    ++ SR P+GR GE NEVSSLVAF
Sbjct: 181 NLACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAF 240

Query: 241 LCLPAASYITGQIVSVDGGFTANGF 265
           LCLPAASYITGQ + VDGGFT NGF
Sbjct: 241 LCLPAASYITGQTICVDGGFTVNGF 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889667.11.0e-13793.41tropinone reductase homolog At5g06060-like [Benincasa hispida][more]
XP_008461702.18.3e-13289.38PREDICTED: tropinone reductase homolog At5g06060-like isoform X1 [Cucumis melo] ... [more]
XP_022976820.12.4e-13189.01tropinone reductase homolog At5g06060 [Cucurbita maxima][more]
XP_023547195.17.0e-13188.64tropinone reductase homolog At5g06060 [Cucurbita pepo subsp. pepo][more]
XP_004150641.17.0e-13188.64tropinone reductase homolog At5g06060 [Cucumis sativus] >KGN50576.1 hypothetical... [more]
Match NameE-valueIdentityDescription
Q9LHT04.0e-10573.26Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW129.3e-9465.25Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9ZW031.3e-9064.53Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Q9ZW042.1e-9063.40Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Q9ZW133.7e-9064.03Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Match NameE-valueIdentityDescription
A0A5D3BV454.0e-13289.38Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CF704.0e-13289.38tropinone reductase homolog At5g06060-like isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1IKI11.2e-13189.01tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC11147708... [more]
A0A0A0KS923.4e-13188.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1[more]
A0A6J1GWZ52.2e-13087.91tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
Match NameE-valueIdentityDescription
AT5G06060.12.9e-10673.26NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29260.16.6e-9565.25NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29150.18.9e-9264.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.22.6e-9164.03NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.19.9e-9162.26NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 167..186
score: 36.78
coord: 94..105
score: 45.16
coord: 147..155
score: 49.39
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 94..105
score: 49.49
coord: 141..157
score: 35.83
coord: 19..36
score: 50.14
coord: 167..186
score: 41.52
coord: 223..243
score: 33.19
coord: 188..205
score: 38.8
NoneNo IPR availableGENE3D3.40.50.720coord: 2..264
e-value: 4.6E-77
score: 260.7
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 26..260
e-value: 5.6E-56
score: 189.7
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 21..212
e-value: 5.5E-17
score: 59.7
NoneNo IPR availablePANTHERPTHR42898:SF69NAD(P)-BINDING ROSSMANN-FOLD PROTEINcoord: 5..267
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 5..267
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 154..182
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 17..262

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
HG10014875HG10014875gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
HG10014875.1-cdsHG10014875.1-cds-Chr02:21223719..21223807CDS
HG10014875.1-cdsHG10014875.1-cds-Chr02:21223900..21224107CDS
HG10014875.1-cdsHG10014875.1-cds-Chr02:21225036..21225252CDS
HG10014875.1-cdsHG10014875.1-cds-Chr02:21225339..21225445CDS
HG10014875.1-cdsHG10014875.1-cds-Chr02:21225828..21226028CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
HG10014875.1HG10014875.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity