HG10010501.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010501.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	gibberellin 20-oxidase-like protein
Location	Chr06: 22731976 .. 22733086 (-)
Sequence length	1011
RNA-Seq Expression	HG10010501.1
Synteny	HG10010501.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATACCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGGTACACACACTTCACAGCACATTCACTTCAGAATCACTCTCTCTCTAATCTAAGATCATAACTTCTATTGTCCTAACAATCTACTTTCATGTGCATAAAGTGAGATATTGCAAGAATATGGAGAAAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTATTGAGCTTTGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACAACCCTCCAGAAACCTTGGGAGACGATGAAGAAGCAGAAGGACTTGGAATGCATACGGATATGAGCTGCGTGACCATTGTATACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTCGACATAGACCCATGTGAAGAATCAGAATCCAAATCCGAATCCGAAGCTCTCGTGGTGAACATTGGCGATTTGCTTCAAGCTTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTAGTTTTGAGGCGGCCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGATTTGAGGACGAGAAGATGGTGTCGGCGCCGGAGGAGTTGGTCGGAGACGACGGTGAACGGCAGTATAAGCCATTTGTTTGCTCAGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTTACTGTGAGAGACTTTGCTGGGAATGGGATTAAGAAGGGTTTTGCGGAAAAATGA

mRNA sequence

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATACCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGAAAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTATTGAGCTTTGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACAACCCTCCAGAAACCTTGGGAGACGATGAAGAAGCAGAAGGACTTGGAATGCATACGGATATGAGCTGCGTGACCATTGTATACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTCGACATAGACCCATGTGAAGAATCAGAATCCAAATCCGAATCCGAAGCTCTCGTGGTGAACATTGGCGATTTGCTTCAAGCTTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTAGTTTTGAGGCGGCCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGATTTGAGGACGAGAAGATGGTGTCGGCGCCGGAGGAGTTGGTCGGAGACGACGGTGAACGGCAGTATAAGCCATTTGTTTGCTCAGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTTACTGTGAGAGACTTTGCTGGGAATGGGATTAAGAAGGGTTTTGCGGAAAAATGA

Coding sequence (CDS)

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATACCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGAAAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTATTGAGCTTTGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACAACCCTCCAGAAACCTTGGGAGACGATGAAGAAGCAGAAGGACTTGGAATGCATACGGATATGAGCTGCGTGACCATTGTATACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTCGACATAGACCCATGTGAAGAATCAGAATCCAAATCCGAATCCGAAGCTCTCGTGGTGAACATTGGCGATTTGCTTCAAGCTTGGAGCAACGACAGGCTGAGGTCGTCGGAGCACAGAGTAGTTTTGAGGCGGCCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGATTTGAGGACGAGAAGATGGTGTCGGCGCCGGAGGAGTTGGTCGGAGACGACGGTGAACGGCAGTATAAGCCATTTGTTTGCTCAGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTTACTGTGAGAGACTTTGCTGGGAATGGGATTAAGAAGGGTTTTGCGGAAAAATGA

Protein sequence

MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDEEAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK

Homology

[Close]

BLAST of HG10010501.1 vs. NCBI nr
Match: XP_038875412.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 649.0 bits (1673), Expect = 2.2e-182
Identity = 322/338 (95.27%), Postives = 325/338 (96.15%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVS ASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF
Sbjct: 1   MSVSNASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPIETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADLLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYGEKMTELSRKIIKNIL+SFGEGFEKTYHEPHFKNCHGYLRINNY  PETL +DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNILMSFGEGFEKTYHEPHFKNCHGYLRINNYTSPETLEEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEES--ESKSESEALVVNIGD 240
           EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEES  ESKSESEALVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESELESKSESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           LLQAW NDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEELVGD GER YKPFV
Sbjct: 241 LLQAWGNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVGDGGERLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKK F EK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKDFEEK 338

[Close]

BLAST of HG10010501.1 vs. NCBI nr
Match: XP_023547730.1 (gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 6.7e-176
Identity = 307/336 (91.37%), Postives = 319/336 (94.94%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+S GEGF+ TYHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ESES+S+SEALVVNIGDLL
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESDSEALVVNIGDLL 240

Query: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 300
           QAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G+R YKPFVCS
Sbjct: 241 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           DYLKFRENDERGKFEKVGFTVRDFAGN I K F EK
Sbjct: 301 DYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 336

[Close]

BLAST of HG10010501.1 vs. NCBI nr
Match: XP_022959365.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-174
Identity = 308/338 (91.12%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+S GEGF+  YHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSE--SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ESES+SE  SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G+R YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           CSDYLKFRENDERGKFEKVGFTVRDFAGN I K F EK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 338

[Close]

BLAST of HG10010501.1 vs. NCBI nr
Match: KAG6575175.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621.7 bits (1602), Expect = 3.7e-174
Identity = 308/338 (91.12%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+S GEGF+  YHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSE--SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ESES+SE  SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G+R YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           CSDYLKFRENDERGKFEKVGFTVRDFAGN I K F EK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKYFEEK 338

[Close]

BLAST of HG10010501.1 vs. NCBI nr
Match: KAG7013737.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.9 bits (1600), Expect = 6.3e-174
Identity = 307/338 (90.83%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+S GEGF+  YHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSE--SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ESES+SE  SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G+R YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           CSDYLKFRENDERGKFEKVGFTVRDFAGN I K F E+
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ 338

[Close]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.0e-99
Identity = 186/330 (56.36%), Postives = 236/330 (71.52%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+SL DACK+WGFF++ NHG+S+D Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDEE----- 186
           GEKMT+L  KI+K IL SFG+     Y+E  F NCHGY RINNY  P    DD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 --AEGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDIDPCEESESKSESEALVVNIGD 246
              EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DI+P        + EALVVN+GD
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP--------KDEALVVNVGD 241

Query: 247 LLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKMVSAPEELVGD-DGERQYK 306
           LL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D K+V AP+E+VG  +G R ++
Sbjct: 242 LLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFR 301

Query: 307 PFVCSDYLKFRENDERGKFEKVGFTVRDFA 326
            F C DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 SFKCGDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.4e-29
Identity = 98/315 (31.11%), Postives = 149/315 (47.30%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           +S       +P++D+S   +P   + + DA K WG F I NHGIS+   + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F  +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQEYGEKMTELSRKIIKNILLSFGEGFE-----KTYHEPHFKNCHGYLRINNYNP 180
           ++  I+QEY ++M +L+ +++  IL S G   E         +   K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGDDEEAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEAL 240
                + E A GL  HTD + +TI++Q  TGGLQV   E  WV ++P            L
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--------PGVL 279

Query: 241 VVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAP-EELVGDDGE 300
           VVNIGDL    SN ++ S  HR  +     +R SIA+ WG     +  AP  +L G    
Sbjct: 280 VVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEP 337

Query: 301 RQYKPFVCSDYLKFR 307
             Y+     +YL+ +
Sbjct: 340 SLYRSITWKEYLQIK 337

[Close]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.4e-27
Identity = 96/305 (31.48%), Postives = 150/305 (49.18%), Query Frame = 0


Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S   ++ DA ++WGFF + NHG+S +    + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSLNT-------YTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F++ A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQEYGEKMTELSRKIIKNILLSFGEGFE-KTYHEPHFKNCHGYLRIN-NYNPPETLGDDE 189
             EY   M E ++ ++K +L   GE    K   +       G  RIN NY P     + E
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYP--ICPNPE 241

Query: 190 EAEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDIDPCEESESKSESEALVVNIGDL 249
              G+G H+D+S +TI+ QD+ GGL V+S + G+WV + P         S +LV+NIGD 
Sbjct: 242 LTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI--------SGSLVINIGDA 301

Query: 250 LQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVC 305
           +Q  SN R +S EHR VL   + NR S+  F   + E ++    E++ +  +  YK  + 
Sbjct: 302 MQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILY 343

[Close]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.1e-27
Identity = 91/314 (28.98%), Postives = 154/314 (49.04%), Query Frame = 0


Query: 3   VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSL 62
           V++    +P++D+S P       ++ DA +KWGFF + NHG+  +  + + + +++ F+L
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD-------FLFNQKS 122
           P E K K    +SL+T            T+R  G +F   A+ + +       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 PHQF-SEILQEYGEKMTELSRKIIKNILLSFGEGFE-KTYHEPHFKNCHGYLRIN-NYNP 182
             QF  +I +    +    S+K+++ +L   G+    K   E       G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLGDDEEAEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDIDPCEESESKSESEA 242
                + +   G+G H+D+S +TI+ QDQ GGL V+S + G WV + P   S        
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS-------- 294

Query: 243 LVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGE 302
            V+NIGD +Q  SN   +S EHR VL     NR S+  F   + E ++    E++ +  E
Sbjct: 295 FVINIGDAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEE 344

Query: 303 RQYKPFVCSDYLKF 306
             Y+  + SDY+K+
Sbjct: 355 PIYRDVLYSDYVKY 344

[Close]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.1e-26
Identity = 89/311 (28.62%), Postives = 148/311 (47.59%), Query Frame = 0


Query: 7   SVELPILDIS-QPLSPSS------LSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEI 66
           S +LP++D S Q L P S       + +  A + +G F      +S +    +     ++
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 67  FSLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQF 126
           F LP  TK +       + Y  H +    +E+L ++  N L         L+        
Sbjct: 64  FELPIPTKQRNVSSKLFHGYLCHNL----YESLGINDANVLEKVNDFTQQLWPDHGNKSI 123

Query: 127 SEILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDD 186
           SE +  + E++ EL   + + I+ SF  G EK Y + H  + +   R+  Y  P    DD
Sbjct: 124 SETIHLFSEQLVELDLMVRRMIMESF--GIEK-YIDEHLNSTYYLTRLMKYTSPPDDDDD 183

Query: 187 EEAE-GLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGD 246
           EE + GL  HTD + +TI++Q Q  GL+VK+ + KW+ + P ++S        ++V +GD
Sbjct: 184 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS--------VLVMVGD 243

Query: 247 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 306
            L A  N RL S  HRV++      R+S   F   +   ++ +PEELV  +  R +KPF 
Sbjct: 244 SLCALLNGRLHSPYHRVIMTGKK-TRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFE 298

Query: 307 CSDYLKFREND 310
            +D+L F + +
Sbjct: 304 YTDFLHFFQTE 298

[Close]

BLAST of HG10010501.1 vs. ExPASy TrEMBL
Match: A0A6J1H632 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111460359 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 308/338 (91.12%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+S GEGF+  YHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSE--SEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ESES+SE  SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G+R YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           CSDYLKFRENDERGKFEKVGFTVRDFAGN I K F EK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 338

[Close]

BLAST of HG10010501.1 vs. ExPASy TrEMBL
Match: A0A6J1KY83 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.5e-173
Identity = 304/336 (90.48%), Postives = 316/336 (94.05%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQ+YG+KMTELSRKIIKNIL+S GEGF+ TYHE HFKNCHGYLRINNY PPETLG+DE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+E    SESEALVVNIGDLL
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDE----SESEALVVNIGDLL 240

Query: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 300
           QAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPEE+VGD G++ YKPFVCS
Sbjct: 241 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAGNGIKKGFAEK 337
           DYLKFRENDERGKFEKVGFTVRDFAGN I K F EK
Sbjct: 301 DYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 332

[Close]

BLAST of HG10010501.1 vs. ExPASy TrEMBL
Match: A0A5D3BVZ1 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001070 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 304/331 (91.84%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISK+ Y+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYGEKMTELSRKIIKN+LLSFGEGFEK YHEPHFKNCHGYLRINNY PPETL DDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 240
           EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+PCE      ESEALVVNIGDLL
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE------ESEALVVNIGDLL 240

Query: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 300
           QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAP+ELVG+ G+R YKPFVCS
Sbjct: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAGNGIKK 332
           DYLKFRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 DYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of HG10010501.1 vs. ExPASy TrEMBL
Match: A0A1S3C8M6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 304/331 (91.84%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISK+ Y+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYGEKMTELSRKIIKN+LLSFGEGFEK YHEPHFKNCHGYLRINNY PPETL DDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 240
           EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+PCE      ESEALVVNIGDLL
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE------ESEALVVNIGDLL 240

Query: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 300
           QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAP+ELVG+ G+R YKPFVCS
Sbjct: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAGNGIKK 332
           DYLKFRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 DYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of HG10010501.1 vs. ExPASy TrEMBL
Match: A0A0A0KB92 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.0e-169
Identity = 296/326 (90.80%), Postives = 309/326 (94.79%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISK+ YNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDE 180
           EILQEYG+KMTELSRKIIKNIL+SFGEGFEK YHEPHFKNCHGYLRINNY PPETL DDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+PCE      ESEALVVNIGDLL
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE------ESEALVVNIGDLL 240

Query: 241 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 300
           QAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEK+VSAPE+LV + GER YKPFVCS
Sbjct: 241 QAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAG 327
           DYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 DYLKFRENDERGKFEKVGFTVTNFAG 320

[Close]

BLAST of HG10010501.1 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.0e-106
Identity = 186/328 (56.71%), Postives = 249/328 (75.91%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           MS   +S++LP+LD++QP+  S LSSL++ACK+WGFF++ NHGISK+ ++K+ S S ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
             P E+KLK+GP+S    YTP +IASP+FE+L VSGP+F  SA++SAD LF      +  
Sbjct: 61  KAPLESKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELR 120

Query: 121 EILQEYGEKMTELSRKIIKNIL-LSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDD 180
           E +QEYG KM ELS+++IK +L ++ G+   K  ++  F NCHGYLR+ NY PP  +   
Sbjct: 121 ETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQ 180

Query: 181 EE-AEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGD 240
           EE  EGLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DI+PC        ++ LVVNIGD
Sbjct: 181 EELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC--------NDFLVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFV 300
           L+QAWSN RLRSSEHRVVLR+  +NR S+AFF  FEDEK++ AP+E+VG+  +R YK F 
Sbjct: 241 LMQAWSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFK 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 327
           CS+YLKFR+++E GKFEK+GFTV+DFAG
Sbjct: 301 CSEYLKFRQSNEEGKFEKIGFTVKDFAG 315

[Close]

BLAST of HG10010501.1 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.4e-100
Identity = 186/330 (56.36%), Postives = 236/330 (71.52%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+SL DACK+WGFF++ NHG+S+D Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDEE----- 186
           GEKMT+L  KI+K IL SFG+     Y+E  F NCHGY RINNY  P    DD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 --AEGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDIDPCEESESKSESEALVVNIGD 246
              EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DI+P        + EALVVN+GD
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP--------KDEALVVNVGD 241

Query: 247 LLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKMVSAPEELVGD-DGERQYK 306
           LL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D K+V AP+E+VG  +G R ++
Sbjct: 242 LLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFR 301

Query: 307 PFVCSDYLKFRENDERGKFEKVGFTVRDFA 326
            F C DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 SFKCGDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of HG10010501.1 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.7e-68
Identity = 124/206 (60.19%), Postives = 159/206 (77.18%), Query Frame = 0


Query: 123 LQEYGEKMTELSRKIIKNIL-LSFGEGFEKTYHEPHFKNCHGYLRINNYNPPETLGDDEE 182
           +QEYG KM ELS+++IK +L ++ G+   K  ++  F NCHGYLR+ NY PP  +   EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 183 -AEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEALVVNIGDLL 242
             EGLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DI+PC        ++ LVVNIGDL+
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC--------NDFLVVNIGDLM 120

Query: 243 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDGERQYKPFVCS 302
           QAWSN RLRSSEHRVVLR+  +NR S+AFF  FEDEK++ AP+E+VG+  +R YK F CS
Sbjct: 121 QAWSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCS 180

Query: 303 DYLKFRENDERGKFEKVGFTVRDFAG 327
           +YLKFR+++E GKFEK+GFTV+DFAG
Sbjct: 181 EYLKFRQSNEEGKFEKIGFTVKDFAG 197

[Close]

BLAST of HG10010501.1 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 9.8e-32
Identity = 99/334 (29.64%), Postives = 158/334 (47.31%), Query Frame = 0


Query: 9   ELPILDISQPLSPSS----LSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIFSLPS 68
           E+P++D+S+   P      +S + DAC+KWGFF + NHG+  D   ++       F LP 
Sbjct: 27  EIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDLPM 86

Query: 69  ETKLK-----IGPLS------SLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQ 128
           E K+K     + P+       + N      +   +F+   V  P+           ++N+
Sbjct: 87  EEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKDPMVI-PSTTDPEDEGLRLVYNK 146

Query: 129 --KSPHQFSEILQEYGEKMTELSRKIIKNILLSFGEGFEKTYHEPHFKNCHGYLRINNYN 188
             +SP  F E  + Y     +L+ K+++ I LS G   E+ +H+ +FK    + RIN Y 
Sbjct: 147 WPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKER-FHD-YFKEQMSFFRINRYP 206

Query: 189 PPETLGDDEEAEGLGMHTDMSCVTIVYQDQTGGLQV-KSSEGKWVDIDPCEESESKSESE 248
           P       + A G+G H D   ++++ QD  GGLQV + S+G W  I P           
Sbjct: 207 P---CPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPV--------PN 266

Query: 249 ALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAPEELVGDDG 308
           ALV+NIG+ ++ W+ND+  S+EHRVV+      R+SI FF     +  V   EELV  + 
Sbjct: 267 ALVINIGNCMEIWTNDKYWSAEHRVVVNTTR-ERYSIPFFLLPSHDVEVKPLEELVSPEN 326

Query: 309 ERQYKPFVCSDYLKFRENDERGKFEKVGFTVRDF 325
             +YK +    +   R   +  K E     + DF
Sbjct: 327 PPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDF 345

[Close]

BLAST of HG10010501.1 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 130.2 bits (326), Expect = 3.1e-30
Identity = 98/315 (31.11%), Postives = 149/315 (47.30%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60
           +S       +P++D+S   +P   + + DA K WG F I NHGIS+   + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F  +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQEYGEKMTELSRKIIKNILLSFGEGFE-----KTYHEPHFKNCHGYLRINNYNP 180
           ++  I+QEY ++M +L+ +++  IL S G   E         +   K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGDDEEAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESESKSESEAL 240
                + E A GL  HTD + +TI++Q  TGGLQV   E  WV ++P            L
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--------PGVL 279

Query: 241 VVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKMVSAP-EELVGDDGE 300
           VVNIGDL    SN ++ S  HR  +     +R SIA+ WG     +  AP  +L G    
Sbjct: 280 VVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEP 337

Query: 301 RQYKPFVCSDYLKFR 307
             Y+     +YL+ +
Sbjct: 340 SLYRSITWKEYLQIK 337

The following BLAST results are available for this feature:

BLAST of HG10010501.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875412.1	2.2e-182	95.27	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
XP_023547730.1	6.7e-176	91.37	gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo]	[more]
XP_022959365.1	2.2e-174	91.12	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]
KAG6575175.1	3.7e-174	91.12	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG7013737.1	6.3e-174	90.83	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of HG10010501.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	9.0e-99	56.36	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C971	4.4e-29	31.11	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9C899	2.4e-27	31.48	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
Q9LHN8	4.1e-27	28.98	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
Q944Z5	2.1e-26	28.62	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]

BLAST of HG10010501.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H632	1.1e-174	91.12	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11146035...	[more]
A0A6J1KY83	1.5e-173	90.48	gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 ...	[more]
A0A5D3BVZ1	1.3e-172	91.84	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C8M6	1.3e-172	91.84	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0KB92	3.0e-169	90.80	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]

BLAST of HG10010501.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	6.0e-106	56.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	6.4e-100	56.36	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	1.7e-68	60.19	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	9.8e-32	29.64	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80330.1	3.1e-30	31.11	gibberellin 3-oxidase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..270 e-value: 1.5E-19 score: 70.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..305 e-value: 2.2E-70 score: 239.8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..72 e-value: 3.9E-15 score: 56.7
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..323
None	No IPR available	PANTHER	PTHR47990:SF52	GIBBERELLIN 3-BETA-DIOXYGENASE 4	coord: 4..323
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..309
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..273 score: 11.562861

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10010501	HG10010501	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10010501.1-cds	HG10010501.1-cds-Chr06:22731976..22732627	CDS
HG10010501.1-cds	HG10010501.1-cds-Chr06:22732728..22733086	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10010501.1	HG10010501.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding