HG10009654.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009654.1 vs. NCBI nr
Match: KGN49808.1 (hypothetical protein Csa_004650 [Cucumis sativus])

HSP 1 Score: 240.0 bits (611), Expect = 1.5e-59
Identity = 126/164 (76.83%), Postives = 137/164 (83.54%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQQSPQATASSPQNLA 60
           MASTSHQTS  M+A SSFI TI+QR+YG+   CTPS+CLP+ IRMQQSPQATA S Q L 
Sbjct: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60

Query: 61  KTILGLTFQAILALFISS-PTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFE 120
           K IL L+FQA+LALFISS PTS PPLL H F AAV ISFAVSFAALFLHNSFPRTA LFE
Sbjct: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120

Query: 121 KIGALFAGIGVCIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           K+GALF+  GVC IASFLLVHQNFAWICW+AC FS IVF LSFK
Sbjct: 121 KVGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 164

BLAST of HG10009654.1 vs. NCBI nr
Match: KAA0050246.1 (putative Ileal sodium/bile acid cotransporter [Cucumis melo var. makuwa] >TYJ98160.1 putative Ileal sodium/bile acid cotransporter [Cucumis melo var. makuwa])

HSP 1 Score: 179.9 bits (455), Expect = 1.8e-41
Identity = 102/153 (66.67%), Postives = 117/153 (76.47%), Query Frame = 0

Query: 12  MNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQQ-SPQATASSPQNLAKTILGLTFQA 71
           MN L SFI  IN+R+  I       + LPICI MQ+ SP  ++ +  N+ KTILGLTFQA
Sbjct: 1   MNELYSFITAINERNPEI-------NSLPICITMQRPSPANSSKTENNVGKTILGLTFQA 60

Query: 72  ILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFEKIGALFAGIGV 131
           +LALFI+SP SSPPLLTHLF AAVLISFAVSFA +FL N FPR A LFEKIGAL A IGV
Sbjct: 61  VLALFITSPNSSPPLLTHLFAAAVLISFAVSFAGIFLQNGFPRIALLFEKIGALIAAIGV 120

Query: 132 CIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           CI+AS LL+HQNFAWI WLA GFS + FVLSF+
Sbjct: 121 CIVAS-LLIHQNFAWISWLASGFSLMAFVLSFR 145

BLAST of HG10009654.1 vs. NCBI nr
Match: KGN49803.1 (hypothetical protein Csa_004681 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 6.9e-41
Identity = 104/164 (63.41%), Postives = 122/164 (74.39%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQ-QSPQATASSPQNL 60
           M S+S Q S  MN L S I +IN+R+  I       + LPICI MQ  SP  ++ +  N+
Sbjct: 1   MESSSQQISIDMNHLYSLITSINERNPEI-------NGLPICIIMQTASPANSSKTENNV 60

Query: 61  AKTILGLTFQAILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFE 120
             TILGLTFQA+LALFI+S TSSPPLLTHLFGAAVLISFAVSF  +FL + FPR A LFE
Sbjct: 61  GTTILGLTFQAVLALFITSSTSSPPLLTHLFGAAVLISFAVSFPGVFLQDGFPRIALLFE 120

Query: 121 KIGALFAGIGVCIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           KIGAL A IGVCI+AS LL+HQNFAWI WLACGFS + F+LSF+
Sbjct: 121 KIGALIAAIGVCILAS-LLIHQNFAWISWLACGFSLMAFLLSFR 156

BLAST of HG10009654.1 vs. NCBI nr
Match: KAG6579132.1 (hypothetical protein SDJN03_23580, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 147.5 bits (371), Expect = 9.9e-32
Identity = 81/131 (61.83%), Postives = 98/131 (74.81%), Query Frame = 0

Query: 32  FCTPSDCLPICIRMQQSPQATASSPQNLAKTILGLTFQAILALFISSPTSSPPLLTHLFG 91
           F TP DCLPI  R ++          N+ K I+GLT QA+LA+FISSP+SSPPLL  LFG
Sbjct: 3   FSTPRDCLPIW-RAREQRLEPDKGHSNVGKAIIGLTSQALLAMFISSPSSSPPLLRVLFG 62

Query: 92  AAVLISFAVSFAALFLHNSFPRTARLFEKIGALFAGIGVCIIASFLLVHQNFAWICWLAC 151
           A + ISF +SFA +FL N+FP+ ARLFEK+GALFA IGV IIASFLL+H+N+AWI  LAC
Sbjct: 63  ATMFISFLLSFAGIFLRNAFPKAARLFEKLGALFAAIGVSIIASFLLMHENYAWISGLAC 122

Query: 152 GFSFIVFVLSF 163
            FS IVF LS+
Sbjct: 123 VFSLIVFGLSY 132

BLAST of HG10009654.1 vs. NCBI nr
Match: XP_022146444.1 (uncharacterized protein LOC111015658 [Momordica charantia])

HSP 1 Score: 144.8 bits (364), Expect = 6.4e-31
Identity = 86/126 (68.25%), Postives = 95/126 (75.40%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIF------CTPSDCLPICIRMQQSPQATAS 60
           MAST  QTS  MNALSSFI  IN+R+ GIS F       TPSDCLPICIRMQ+ P A +S
Sbjct: 1   MASTPQQTSVDMNALSSFITAINERNLGISSFTWGGGTSTPSDCLPICIRMQRPPPAKSS 60

Query: 61  SPQNLAKTILGLTFQAILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRT 120
             Q+L KTILGLTFQA+LALFIS P+S P L T LFGAAVLISFAVSFA LFL  ++PR 
Sbjct: 61  --QSLGKTILGLTFQAVLALFISLPSSPPQLPTLLFGAAVLISFAVSFAGLFLQTAYPRM 120

BLAST of HG10009654.1 vs. ExPASy TrEMBL
Match: A0A0A0KJP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G137410 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 7.1e-60
Identity = 126/164 (76.83%), Postives = 137/164 (83.54%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQQSPQATASSPQNLA 60
           MASTSHQTS  M+A SSFI TI+QR+YG+   CTPS+CLP+ IRMQQSPQATA S Q L 
Sbjct: 1   MASTSHQTSLDMSAFSSFIRTISQRNYGLGCCCTPSNCLPVFIRMQQSPQATADSSQKLG 60

Query: 61  KTILGLTFQAILALFISS-PTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFE 120
           K IL L+FQA+LALFISS PTS PPLL H F AAV ISFAVSFAALFLHNSFPRTA LFE
Sbjct: 61  KIILSLSFQAVLALFISSPPTSPPPLLIHFFAAAVFISFAVSFAALFLHNSFPRTAHLFE 120

Query: 121 KIGALFAGIGVCIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           K+GALF+  GVC IASFLLVHQNFAWICW+AC FS IVF LSFK
Sbjct: 121 KVGALFSAFGVCFIASFLLVHQNFAWICWVACTFSIIVFALSFK 164

BLAST of HG10009654.1 vs. ExPASy TrEMBL
Match: A0A5A7U7U1 (Putative Ileal sodium/bile acid cotransporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00420 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 8.7e-42
Identity = 102/153 (66.67%), Postives = 117/153 (76.47%), Query Frame = 0

Query: 12  MNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQQ-SPQATASSPQNLAKTILGLTFQA 71
           MN L SFI  IN+R+  I       + LPICI MQ+ SP  ++ +  N+ KTILGLTFQA
Sbjct: 1   MNELYSFITAINERNPEI-------NSLPICITMQRPSPANSSKTENNVGKTILGLTFQA 60

Query: 72  ILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFEKIGALFAGIGV 131
           +LALFI+SP SSPPLLTHLF AAVLISFAVSFA +FL N FPR A LFEKIGAL A IGV
Sbjct: 61  VLALFITSPNSSPPLLTHLFAAAVLISFAVSFAGIFLQNGFPRIALLFEKIGALIAAIGV 120

Query: 132 CIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           CI+AS LL+HQNFAWI WLA GFS + FVLSF+
Sbjct: 121 CIVAS-LLIHQNFAWISWLASGFSLMAFVLSFR 145

BLAST of HG10009654.1 vs. ExPASy TrEMBL
Match: A0A0A0KJN8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136870 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.3e-41
Identity = 104/164 (63.41%), Postives = 122/164 (74.39%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIFCTPSDCLPICIRMQ-QSPQATASSPQNL 60
           M S+S Q S  MN L S I +IN+R+  I       + LPICI MQ  SP  ++ +  N+
Sbjct: 1   MESSSQQISIDMNHLYSLITSINERNPEI-------NGLPICIIMQTASPANSSKTENNV 60

Query: 61  AKTILGLTFQAILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRTARLFE 120
             TILGLTFQA+LALFI+S TSSPPLLTHLFGAAVLISFAVSF  +FL + FPR A LFE
Sbjct: 61  GTTILGLTFQAVLALFITSSTSSPPLLTHLFGAAVLISFAVSFPGVFLQDGFPRIALLFE 120

Query: 121 KIGALFAGIGVCIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           KIGAL A IGVCI+AS LL+HQNFAWI WLACGFS + F+LSF+
Sbjct: 121 KIGALIAAIGVCILAS-LLIHQNFAWISWLACGFSLMAFLLSFR 156

BLAST of HG10009654.1 vs. ExPASy TrEMBL
Match: A0A6J1CY58 (uncharacterized protein LOC111015658 OS=Momordica charantia OX=3673 GN=LOC111015658 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 3.1e-31
Identity = 86/126 (68.25%), Postives = 95/126 (75.40%), Query Frame = 0

Query: 1   MASTSHQTSFYMNALSSFIPTINQRSYGISIF------CTPSDCLPICIRMQQSPQATAS 60
           MAST  QTS  MNALSSFI  IN+R+ GIS F       TPSDCLPICIRMQ+ P A +S
Sbjct: 1   MASTPQQTSVDMNALSSFITAINERNLGISSFTWGGGTSTPSDCLPICIRMQRPPPAKSS 60

Query: 61  SPQNLAKTILGLTFQAILALFISSPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRT 120
             Q+L KTILGLTFQA+LALFIS P+S P L T LFGAAVLISFAVSFA LFL  ++PR 
Sbjct: 61  --QSLGKTILGLTFQAVLALFISLPSSPPQLPTLLFGAAVLISFAVSFAGLFLQTAYPRM 120

BLAST of HG10009654.1 vs. ExPASy TrEMBL
Match: A0A0A0KQ03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136900 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.0e-25
Identity = 83/169 (49.11%), Postives = 106/169 (62.72%), Query Frame = 0

Query: 1   MAST-SHQTSFYMNALSSFIPTINQRSYGISIFCT----PSDCLPICIRMQQSPQATASS 60
           MA T  H+ S  MN L+S I  +  R+ GIS  CT     SDCLPI I+MQ+   +  +S
Sbjct: 1   MAETPPHEISLDMNKLNSLIFAVTNRNPGIS-SCTWGGAASDCLPIYIKMQR--PSPVNS 60

Query: 61  PQNLAKTILGLTFQAILALFIS-SPTSSPPLLTHLFGAAVLISFAVSFAALFLHNSFPRT 120
           PQ    T L LTFQAI+ LF+S +P+SS PL + LF A +L SF  S+  + L   FP+T
Sbjct: 61  PQ-FGNTFLSLTFQAIVGLFLSLNPSSSSPLPSRLFAAVMLTSFIFSYDGVILQKPFPKT 120

Query: 121 ARLFEKIGALFAGIGVCIIASFLLVHQNFAWICWLACGFSFIVFVLSFK 164
           A+L +  GALFA IG CII S LL++ NF WICWLA G     F++SFK
Sbjct: 121 AQLLQTFGALFAAIGTCIIGS-LLLYPNFTWICWLAAGLILPAFIISFK 164