HG10009557.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10009557.1
Type	mRNA
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	B3 domain-containing protein At2g31420-like
Location	Chr06: 7446614 .. 7447621 (-)
Sequence length	537
RNA-Seq Expression	HG10009557.1
Synteny	HG10009557.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGTAAGTAGAAAATCTTATATAGAAGAAGATTTTGACGGGGTTTGACCGGAGTTTGACTCTGTTTCTTGAAAGTAAACTGAAATAAATCTGGTAAAGATTTCGTAATATTTTCCTAAACTAAATCTCAGCCTAACGGTAACAAATTGAGTCACAAAAGGAATTAAAATCAAATTAGGAATGAAATTGGTATAAGGTTTTAGTTTACAGAGATAATCTGGAGAGTGAAGTCCGACTAAGTTATTTTACTTCCACCGCTCATCTCTGTTCAATTAATTTCTTCTACTCTGTTTAGATTTCAAGTTTTGTGTGTTTAAGAAATAACAGTGGGAAAGAGGACCGCAGTTTAAGGCTTCAGAATGTGTCTTGATCTTCCTGATTTAAACGAAATTTACATACCTCGAAGAAGACGGGGATCTTCCGGTACCGGAGAGAAAGAACAACCCAAGACTGAAATCGACCTTCCCTTGAAAGAACAACCCAAGACTGAAATCGAAACCTCCGACGCCGACGCCGAAAACTACTACTAAGAAACAGAGGAAGAAGAAGGCAAAACAGAGTGAGTATCCGTCGATTCCTGAAATGCCGGCGGCGATGAGAGAACGGATTGTAGAGATGGGTGGGTATGAAATACGACTGGTGATACAGAAACAACTCCAAGACACGGATTTAAACAAGAACCATGGGCGTTTATCCCTGCCAGCAAAGAAAATGCTGTTTGATTTTGCAACAGAGGAGGAGAGAAAGTTGCTGAGTGAACAAGAAAATAAGAACAAGAAGGGGATGAACGTAGTGATTGTGGATGATATTGTTGGAGAAAGAAGCCTTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATTCGTTTGTGGAAGAAACGGGATTGAAAAGTGGACAACATATCCAACTTTGGTGCTTTAGAAAAGATGATGAATTTGAGGCTTCTCGTCTTTGCTTTGCTATGGTTAAGTTAAATTAG

mRNA sequence

ATGCAAAACAACCCAAGACTGAAATCGAAACCTCCGACGCCGACGCCGAAAACTACTACTAAGAAACAGAGGAAGAAGAAGGCAAAACAGAGTGAGTATCCGTCGATTCCTGAAATGCCGGCGGCGATGAGAGAACGGATTGTAGAGATGGGTGGGTATGAAATACGACTGGTGATACAGAAACAACTCCAAGACACGGATTTAAACAAGAACCATGGGCGTTTATCCCTGCCAGCAAAGAAAATGCTGTTTGATTTTGCAACAGAGGAGGAGAGAAAGTTGCTGAGTGAACAAGAAAATAAGAACAAGAAGGGGATGAACGTAGTGATTGTGGATGATATTGTTGGAGAAAGAAGCCTTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATTCGTTTGTGGAAGAAACGGGATTGAAAAGTGGACAACATATCCAACTTTGGTGCTTTAGAAAAGATGATGAATTTGAGGCTTCTCGTCTTTGCTTTGCTATGGTTAAGTTAAATTAG

Coding sequence (CDS)

ATGCAAAACAACCCAAGACTGAAATCGAAACCTCCGACGCCGACGCCGAAAACTACTACTAAGAAACAGAGGAAGAAGAAGGCAAAACAGAGTGAGTATCCGTCGATTCCTGAAATGCCGGCGGCGATGAGAGAACGGATTGTAGAGATGGGTGGGTATGAAATACGACTGGTGATACAGAAACAACTCCAAGACACGGATTTAAACAAGAACCATGGGCGTTTATCCCTGCCAGCAAAGAAAATGCTGTTTGATTTTGCAACAGAGGAGGAGAGAAAGTTGCTGAGTGAACAAGAAAATAAGAACAAGAAGGGGATGAACGTAGTGATTGTGGATGATATTGTTGGAGAAAGAAGCCTTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATTCGTTTGTGGAAGAAACGGGATTGAAAAGTGGACAACATATCCAACTTTGGTGCTTTAGAAAAGATGATGAATTTGAGGCTTCTCGTCTTTGCTTTGCTATGGTTAAGTTAAATTAG

Protein sequence

MQNNPRLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQLQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLKKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN

Homology

[Close]

BLAST of HG10009557.1 vs. NCBI nr
Match: XP_038876237.1 (putative B3 domain-containing protein At3g24850 [Benincasa hispida])

HSP 1 Score: 290.0 bits (741), Expect = 1.4e-74
Identity = 141/175 (80.57%), Postives = 157/175 (89.71%), Query Frame = 0


Query: 4   NPRLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQL 63
           N   + KPPTPTP T TKKQRKKKAKQS+YP I EMP AMR+RIVEMGGY I LVIQKQL
Sbjct: 73  NTHKRKKPPTPTPTTMTKKQRKKKAKQSKYPWITEMPVAMRDRIVEMGGYGINLVIQKQL 132

Query: 64  QDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLK 123
           QDTDLNKN GRLSLPA+K+LFDF TE+E+ LLS++ENK KKGMNV+I+DD++ ER +CLK
Sbjct: 133 QDTDLNKNQGRLSLPAEKLLFDFTTEDEKILLSQRENKKKKGMNVLIMDDVLEERRICLK 192

Query: 124 KWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
            WKIGSGDVYCLMTQWNSFVEETGLKSG+HIQ+WCFRKDDEFEASRLCFAMVKLN
Sbjct: 193 MWKIGSGDVYCLMTQWNSFVEETGLKSGEHIQIWCFRKDDEFEASRLCFAMVKLN 247

[Close]

BLAST of HG10009557.1 vs. NCBI nr
Match: XP_038905532.1 (putative B3 domain-containing protein At3g24850 [Benincasa hispida])

HSP 1 Score: 280.8 bits (717), Expect = 8.2e-72
Identity = 140/179 (78.21%), Postives = 156/179 (87.15%), Query Frame = 0


Query: 1   MQNNPRLKSKP--PTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLV 60
           + NN   +  P  PTPTP T T+KQRKKKAKQS+YPSIPEMPAA+R+RIVEMGGYEIRLV
Sbjct: 23  ISNNTHERKSPLTPTPTPMTATRKQRKKKAKQSKYPSIPEMPAALRDRIVEMGGYEIRLV 82

Query: 61  IQKQLQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGER 120
           IQKQL+DTDLNKNHGRLS+P KK+LFDF TEEERKLLSE EN+NKKG+N VI+DD V ER
Sbjct: 83  IQKQLEDTDLNKNHGRLSMPIKKLLFDFTTEEERKLLSEVENQNKKGINTVIIDD-VEER 142

Query: 121 SLCLKKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
            +CLKKWKIGSGDVYCL TQWNS VEETGLKSGQHIQ+W FRKDDE+E  R+C AMVKL
Sbjct: 143 MICLKKWKIGSGDVYCLTTQWNSLVEETGLKSGQHIQIWSFRKDDEYETYRVCLAMVKL 200

[Close]

BLAST of HG10009557.1 vs. NCBI nr
Match: XP_038874960.1 (B3 domain-containing protein At2g31420-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.6e-70
Identity = 134/173 (77.46%), Postives = 154/173 (89.02%), Query Frame = 0


Query: 6   RLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQLQD 65
           R K   P PTP T T+KQ  KKAK+S+ PSIPE+PAAMR+RIV+MGGY I LVIQKQLQD
Sbjct: 66  RKKHPTPMPTPTTMTEKQMNKKAKKSKSPSIPEIPAAMRDRIVKMGGYGINLVIQKQLQD 125

Query: 66  TDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLKKW 125
           TDLNKN GRLSLPA+K+LFDFATEEE+KLLS++ENK KKGMNV+I+D++  ERS+CLKKW
Sbjct: 126 TDLNKNQGRLSLPAEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEERSICLKKW 185

Query: 126 KIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
           KIGSGDVYCLMTQWN+F EE GLKSGQHIQ+WCFR+DDEFEASRLCF MVKL+
Sbjct: 186 KIGSGDVYCLMTQWNAFAEEKGLKSGQHIQIWCFRRDDEFEASRLCFVMVKLD 238

[Close]

BLAST of HG10009557.1 vs. NCBI nr
Match: XP_038874962.1 (putative B3 domain-containing protein At5g35780 [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 3.4e-70
Identity = 135/176 (76.70%), Postives = 153/176 (86.93%), Query Frame = 0


Query: 3   NNPRLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQ 62
           N    + KP  PTP T TKKQ  KKAK+S+  SI EMPAAMR+RIV+MGGYEI LVIQKQ
Sbjct: 72  NKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINLVIQKQ 131

Query: 63  LQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCL 122
           LQDTDLNKN GRLSLP +K+LFDFATEEE+KLLS++ENK KKGMNV+I+D++  ER +CL
Sbjct: 132 LQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEERRICL 191

Query: 123 KKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
           KKWKIGSGDVYCLMTQWN+F EETGLKSGQHIQ+WCFR+DDEFEASRLCF MVKLN
Sbjct: 192 KKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMVKLN 247

[Close]

BLAST of HG10009557.1 vs. NCBI nr
Match: XP_038907053.1 (B3 domain-containing protein At2g31420-like [Benincasa hispida])

HSP 1 Score: 270.0 bits (689), Expect = 1.4e-68
Identity = 137/170 (80.59%), Postives = 150/170 (88.24%), Query Frame = 0


Query: 8   KSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQLQDTD 67
           K K P  TPKT TKKQRKKKAKQS+YPSIPEMP A+R+RIVEMGGYEIRLVIQKQL+DTD
Sbjct: 76  KRKNPL-TPKTATKKQRKKKAKQSKYPSIPEMPEALRDRIVEMGGYEIRLVIQKQLEDTD 135

Query: 68  LNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLKKWKI 127
           L+KNHGRLS+P KK+LFDF TEEERKLLSE EN+NKKG+N VI+DD V ER +CLKKWKI
Sbjct: 136 LSKNHGRLSMPIKKLLFDFTTEEERKLLSEVENQNKKGINTVIIDD-VEERMICLKKWKI 195

Query: 128 GSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
           GSGDVYCL TQWNS VEETGLK GQHIQ+W FRKDDE EA  +CFAMVKL
Sbjct: 196 GSGDVYCLTTQWNSLVEETGLKVGQHIQIWSFRKDDENEAYHVCFAMVKL 243

[Close]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-12
Identity = 53/163 (32.52%), Postives = 83/163 (50.92%), Query Frame = 0


Query: 24  RKKKAKQSEY--PSIPEMPAAMRERIVEMGGY-EIRLVIQKQLQDTDLNKNHGRLSLPAK 83
           R+KK    E+  P  P+ P  + + +  M G  + +L+I+K L   D++    RLS+P  
Sbjct: 146 RRKKTTSYEFVPPKHPQEPQWLLQVMSRMNGAGDPKLIIEKNLDSNDVDPRQNRLSIPIN 205

Query: 84  KML-FDFATEEERKLLSEQE--NKNKKGMNVVIVDDIVGERSLCLKKW--KIGSGDVY-- 143
            ++  DF T +E +L+ E E  N+   G+   +VD    + ++  K+W     SG  Y  
Sbjct: 206 TVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQRTKKWNMGFKQWFMTTDSGSSYWS 265

Query: 144 -CLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMV 176
             L  +W++ VE  GLK G  I LW FR +D      LCFA+V
Sbjct: 266 FVLRGEWSNVVETNGLKEGDKISLWSFRSND-----ILCFALV 303

[Close]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot
Match: Q8RV83 (B3 domain-containing protein At2g31720 OS=Arabidopsis thaliana OX=3702 GN=ARF70 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-10
Identity = 37/119 (31.09%), Postives = 64/119 (53.78%), Query Frame = 0


Query: 49  EMGGYEIRLVIQKQLQDTDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENK---NKK 108
           E+ G + +L+  K L ++D+++   RL +P K++L  DF  EEE + +     K   + K
Sbjct: 139 EVNGMDAKLIFVKVLPNSDVDELQTRLMMPWKQILDMDFLNEEELEKIDRHHKKISASDK 198

Query: 109 GMNVVIVDDIVGERSLCLKKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDD 164
           G +V++V+    +R L LK+W + S   Y L   WN  V +  L+ G  +++W F   D
Sbjct: 199 GADVIVVNSKGLQRKLKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSFHSPD 257

[Close]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot
Match: Q9XIP5 (Putative B3 domain-containing protein At2g27410 OS=Arabidopsis thaliana OX=3702 GN=At2g27410 PE=3 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.6e-09
Identity = 58/180 (32.22%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 6   RLKSKPPTPTPKTTTKKQRKKKAKQSEYP---SIPEMPAAMRERIVEMGGYEIRLVIQKQ 65
           R K K P    K + KK RK      E P   + PE    +  R  E  GY  +L+  +Q
Sbjct: 37  RSKEKRPINVEKRSHKK-RKIICPLEEEPIQTTPPEWLLNVMRR--EENGYNPKLISTRQ 96

Query: 66  LQDTDLNKNHGRLSLPAKKMLF-DFATEEERKLLSEQENK-NKKGMNVVIVDDIVGERSL 125
           L  TDL K   RLS+P K++   DF TE+E +++ E   K    G+ V  VD  + +  L
Sbjct: 97  LYKTDLKKTEARLSVPFKQVKTPDFLTEDETRIIHENAMKIRDNGVPVNFVDPELNKHVL 156

Query: 126 CLKKWKIGSGDVYCLMTQWNSFVE--ETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
            L+KWK+    +Y  +  W + ++  +T  K      LW FR        +LCFA+   N
Sbjct: 157 ELRKWKMKGNWIYVFVKGWKNVLDACKTLFKEDDVYPLWSFRSG----TGKLCFALTPKN 209

[Close]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot
Match: Q9SJ98 (B3 domain-containing protein At2g24670 OS=Arabidopsis thaliana OX=3702 GN=At2g24670 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.9e-07
Identity = 45/174 (25.86%), Postives = 85/174 (48.85%), Query Frame = 0


Query: 11  PPTPTPKTTTKKQRKKKAKQSEYPSIPE-MPAAMRERIVEMGGYEI----RLVIQKQLQD 70
           PP   P    K ++ K++  +      E  P  +   +    G ++    + +I K+L  
Sbjct: 80  PPMSPPMEQRKSKKAKRSTDASSSKTREPTPGWLISLMRSKNGGDVEDNSKKIIDKELFQ 139

Query: 71  TDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENK-NKKGMNVVIVDDIVGERSLCLK 130
           TD++ +  RLS+P  +++  +F   EE++ + E  N+  K+G++ ++VD  + E  + L+
Sbjct: 140 TDVDPHQSRLSIPVSQIVELEFLNHEEKRAIEEDANRVRKEGVDAILVDSHLREFPVNLR 199

Query: 131 KWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
              +    +Y L+  WN  V +  L+   +I+LW F  DD      L FA+V L
Sbjct: 200 LRDMRGILLYNLVAGWNQVVTDCLLEENTNIRLWSFHADD-----TLYFALVPL 248

[Close]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot
Match: Q9FFX2 (B3 domain-containing protein At5g38490 OS=Arabidopsis thaliana OX=3702 GN=At5g38490 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.3e-07
Identity = 58/195 (29.74%), Postives = 92/195 (47.18%), Query Frame = 0


Query: 4   NPRLKSKPPTP--TPKTTTKK----QRKK-KAKQSEYPSIPEMPAAMRERIVEMGGY--- 63
           NP  +S  P+   T KT  K+    QRK  K K+ +   +P +   M E I ++  Y   
Sbjct: 172 NPNYQSSSPSSCLTGKTNRKRRAVEQRKSGKVKKVKVSPLPRLCTEMPEWIFQVMRYMNA 231

Query: 64  ---EIRLVIQKQLQDTDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENKNKK----- 123
                RL+ ++ L  +D+N N  RL +P +K++  DF T  E + + + E+ +++     
Sbjct: 232 DAETPRLIFERTLFKSDVNSNLSRLLMPFQKLIRNDFLTPAECRAMQKDEDNDEEDDENI 291

Query: 124 GMNVVIVDDIVGERSLCLKKWKI----GSGDV-YCLMTQWNSFVEETGLKSGQHIQLWCF 175
           G+  V+V+    +  L  K W +    G G + Y L   WN  V+ + LK G  I LW F
Sbjct: 292 GVGTVLVNQRFQKWGLRFKIWAMEKDSGHGTLNYTLNWGWNDVVKSSSLKVGDKISLWTF 351

[Close]

BLAST of HG10009557.1 vs. ExPASy TrEMBL
Match: A0A5D3CT22 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00310 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 8.0e-65
Identity = 132/180 (73.33%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 2   QNNPRLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQK 61
           Q N   K K P  T     K++RKKK KQSEYPSIPEMP  MR+RI EMGGYEI LVIQK
Sbjct: 22  QINKTHKRKNPPRTNTAIKKQRRKKKVKQSEYPSIPEMPTTMRDRIEEMGGYEINLVIQK 81

Query: 62  QLQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGE-RSL 121
           QL DTDLNKN GRLS+P KK+ FDF TEEER LLS+QENKNKKG+NV+I++++V + RS+
Sbjct: 82  QLTDTDLNKNEGRLSMPKKKLAFDFTTEEERNLLSQQENKNKKGINVMIMNNMVEDIRSI 141

Query: 122 CLKKWKIGS--GDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
           CLKKWKIGS  GDVYCLMTQWN+FVEETGLKSGQHIQLWCFRKD EFE  RLCFA+V+LN
Sbjct: 142 CLKKWKIGSGNGDVYCLMTQWNAFVEETGLKSGQHIQLWCFRKDYEFEPPRLCFAVVRLN 201

[Close]

BLAST of HG10009557.1 vs. ExPASy TrEMBL
Match: A0A1S3CSS4 (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504467 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.3e-64
Identity = 131/180 (72.78%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 2   QNNPRLKSKPPTPTPKTTTKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQK 61
           Q N   K K P  T     K++RKKK +QSEYPSIPEMP  MR+RI EMGGYEI LVIQK
Sbjct: 68  QINKTHKRKNPPRTNTAIKKQRRKKKVEQSEYPSIPEMPTTMRDRIEEMGGYEINLVIQK 127

Query: 62  QLQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGE-RSL 121
           QL DTDLNKN GRLS+P KK+ FDF TEEER LLS+QENKNKKG+NV+I++++V + RS+
Sbjct: 128 QLTDTDLNKNEGRLSMPKKKLAFDFTTEEERNLLSQQENKNKKGINVMIMNNMVEDIRSI 187

Query: 122 CLKKWKIGS--GDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
           CLKKWKIGS  GDVYCLMTQWN+FVEETGLKSGQHIQLWCFRKD EFE  RLCFA+V+LN
Sbjct: 188 CLKKWKIGSGNGDVYCLMTQWNAFVEETGLKSGQHIQLWCFRKDYEFEPPRLCFAVVRLN 247

[Close]

BLAST of HG10009557.1 vs. ExPASy TrEMBL
Match: A0A5A7TY94 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00280 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.2e-60
Identity = 122/169 (72.19%), Postives = 142/169 (84.02%), Query Frame = 0


Query: 11  PPTPTPKTT--TKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQLQDTDL 70
           PPT T  +T  TKKQRKK  KQ E+PS   MP AMR+RI+EMGGYEIRLVIQKQL DTDL
Sbjct: 100 PPTLTEASTSRTKKQRKKYVKQIEHPS---MPVAMRDRILEMGGYEIRLVIQKQLTDTDL 159

Query: 71  NKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLKKWKIG 130
           NKNHGRLS+  K++ FDFATEEE KLLSEQENKNK+G+NV+++DD++ ER LCLKKWKIG
Sbjct: 160 NKNHGRLSMNTKQLSFDFATEEETKLLSEQENKNKRGINVMMLDDVLEERMLCLKKWKIG 219

Query: 131 SGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
           SG+VYCLMT+WN  VEE G K G+ IQ+W FRKDDE+EA RLCFA+VKL
Sbjct: 220 SGEVYCLMTRWNLMVEERGFKGGEEIQVWSFRKDDEYEAHRLCFALVKL 265

[Close]

BLAST of HG10009557.1 vs. ExPASy TrEMBL
Match: A0A1S4E600 (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504562 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.2e-60
Identity = 122/169 (72.19%), Postives = 142/169 (84.02%), Query Frame = 0


Query: 11  PPTPTPKTT--TKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQKQLQDTDL 70
           PPT T  +T  TKKQRKK  KQ E+PS   MP AMR+RI+EMGGYEIRLVIQKQL DTDL
Sbjct: 100 PPTLTEASTSRTKKQRKKYVKQIEHPS---MPVAMRDRILEMGGYEIRLVIQKQLTDTDL 159

Query: 71  NKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSLCLKKWKIG 130
           NKNHGRLS+  K++ FDFATEEE KLLSEQENKNK+G+NV+++DD++ ER LCLKKWKIG
Sbjct: 160 NKNHGRLSMNTKQLSFDFATEEETKLLSEQENKNKRGINVMMLDDVLEERMLCLKKWKIG 219

Query: 131 SGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
           SG+VYCLMT+WN  VEE G K G+ IQ+W FRKDDE+EA RLCFA+VKL
Sbjct: 220 SGEVYCLMTRWNLMVEERGFKGGEEIQVWSFRKDDEYEAHRLCFALVKL 265

[Close]

BLAST of HG10009557.1 vs. ExPASy TrEMBL
Match: A0A5A7TWX2 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00300 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 3.5e-60
Identity = 122/177 (68.93%), Postives = 143/177 (80.79%), Query Frame = 0


Query: 3   NNPRLKSKPPTPTPKTT--TKKQRKKKAKQSEYPSIPEMPAAMRERIVEMGGYEIRLVIQ 62
           N    +  PP PT  +T  TKKQRKK   QSE   IP MP  MR+RI+EMGGYEIRLVIQ
Sbjct: 75  NKTHKRKNPPIPTEASTSKTKKQRKKNVDQSE---IPSMPVTMRDRILEMGGYEIRLVIQ 134

Query: 63  KQLQDTDLNKNHGRLSLPAKKMLFDFATEEERKLLSEQENKNKKGMNVVIVDDIVGERSL 122
           KQL DTDLNKNHGRLS+  K++ FDFATEEE +LLSEQENKNKKG+NV+I+DD++ ER L
Sbjct: 135 KQLTDTDLNKNHGRLSMNKKQLSFDFATEEESELLSEQENKNKKGINVMILDDVLEERML 194

Query: 123 CLKKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
           CLKKWKIGSG+VYCLMT+WN  VE+ G +SG+ IQ+W FRKDDE+EA RLCFA+VKL
Sbjct: 195 CLKKWKIGSGEVYCLMTKWNLMVEKRGFESGEEIQVWSFRKDDEYEAHRLCFALVKL 248

[Close]

BLAST of HG10009557.1 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-13
Identity = 53/163 (32.52%), Postives = 83/163 (50.92%), Query Frame = 0


Query: 24  RKKKAKQSEY--PSIPEMPAAMRERIVEMGGY-EIRLVIQKQLQDTDLNKNHGRLSLPAK 83
           R+KK    E+  P  P+ P  + + +  M G  + +L+I+K L   D++    RLS+P  
Sbjct: 146 RRKKTTSYEFVPPKHPQEPQWLLQVMSRMNGAGDPKLIIEKNLDSNDVDPRQNRLSIPIN 205

Query: 84  KML-FDFATEEERKLLSEQE--NKNKKGMNVVIVDDIVGERSLCLKKW--KIGSGDVY-- 143
            ++  DF T +E +L+ E E  N+   G+   +VD    + ++  K+W     SG  Y  
Sbjct: 206 TVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQRTKKWNMGFKQWFMTTDSGSSYWS 265

Query: 144 -CLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMV 176
             L  +W++ VE  GLK G  I LW FR +D      LCFA+V
Sbjct: 266 FVLRGEWSNVVETNGLKEGDKISLWSFRSND-----ILCFALV 303

[Close]

BLAST of HG10009557.1 vs. TAIR 10
Match: AT2G31720.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 66.6 bits (161), Expect = 2.3e-11
Identity = 37/119 (31.09%), Postives = 64/119 (53.78%), Query Frame = 0


Query: 49  EMGGYEIRLVIQKQLQDTDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENK---NKK 108
           E+ G + +L+  K L ++D+++   RL +P K++L  DF  EEE + +     K   + K
Sbjct: 139 EVNGMDAKLIFVKVLPNSDVDELQTRLMMPWKQILDMDFLNEEELEKIDRHHKKISASDK 198

Query: 109 GMNVVIVDDIVGERSLCLKKWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDD 164
           G +V++V+    +R L LK+W + S   Y L   WN  V +  L+ G  +++W F   D
Sbjct: 199 GADVIVVNSKGLQRKLKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSFHSPD 257

[Close]

BLAST of HG10009557.1 vs. TAIR 10
Match: AT2G27410.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-10
Identity = 58/180 (32.22%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 6   RLKSKPPTPTPKTTTKKQRKKKAKQSEYP---SIPEMPAAMRERIVEMGGYEIRLVIQKQ 65
           R K K P    K + KK RK      E P   + PE    +  R  E  GY  +L+  +Q
Sbjct: 37  RSKEKRPINVEKRSHKK-RKIICPLEEEPIQTTPPEWLLNVMRR--EENGYNPKLISTRQ 96

Query: 66  LQDTDLNKNHGRLSLPAKKMLF-DFATEEERKLLSEQENK-NKKGMNVVIVDDIVGERSL 125
           L  TDL K   RLS+P K++   DF TE+E +++ E   K    G+ V  VD  + +  L
Sbjct: 97  LYKTDLKKTEARLSVPFKQVKTPDFLTEDETRIIHENAMKIRDNGVPVNFVDPELNKHVL 156

Query: 126 CLKKWKIGSGDVYCLMTQWNSFVE--ETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKLN 179
            L+KWK+    +Y  +  W + ++  +T  K      LW FR        +LCFA+   N
Sbjct: 157 ELRKWKMKGNWIYVFVKGWKNVLDACKTLFKEDDVYPLWSFRSG----TGKLCFALTPKN 209

[Close]

BLAST of HG10009557.1 vs. TAIR 10
Match: AT2G24670.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-08
Identity = 45/174 (25.86%), Postives = 85/174 (48.85%), Query Frame = 0


Query: 11  PPTPTPKTTTKKQRKKKAKQSEYPSIPE-MPAAMRERIVEMGGYEI----RLVIQKQLQD 70
           PP   P    K ++ K++  +      E  P  +   +    G ++    + +I K+L  
Sbjct: 80  PPMSPPMEQRKSKKAKRSTDASSSKTREPTPGWLISLMRSKNGGDVEDNSKKIIDKELFQ 139

Query: 71  TDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENK-NKKGMNVVIVDDIVGERSLCLK 130
           TD++ +  RLS+P  +++  +F   EE++ + E  N+  K+G++ ++VD  + E  + L+
Sbjct: 140 TDVDPHQSRLSIPVSQIVELEFLNHEEKRAIEEDANRVRKEGVDAILVDSHLREFPVNLR 199

Query: 131 KWKIGSGDVYCLMTQWNSFVEETGLKSGQHIQLWCFRKDDEFEASRLCFAMVKL 178
              +    +Y L+  WN  V +  L+   +I+LW F  DD      L FA+V L
Sbjct: 200 LRDMRGILLYNLVAGWNQVVTDCLLEENTNIRLWSFHADD-----TLYFALVPL 248

[Close]

BLAST of HG10009557.1 vs. TAIR 10
Match: AT5G38490.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 55.5 bits (132), Expect = 5.2e-08
Identity = 58/195 (29.74%), Postives = 92/195 (47.18%), Query Frame = 0


Query: 4   NPRLKSKPPTP--TPKTTTKK----QRKK-KAKQSEYPSIPEMPAAMRERIVEMGGY--- 63
           NP  +S  P+   T KT  K+    QRK  K K+ +   +P +   M E I ++  Y   
Sbjct: 172 NPNYQSSSPSSCLTGKTNRKRRAVEQRKSGKVKKVKVSPLPRLCTEMPEWIFQVMRYMNA 231

Query: 64  ---EIRLVIQKQLQDTDLNKNHGRLSLPAKKML-FDFATEEERKLLSEQENKNKK----- 123
                RL+ ++ L  +D+N N  RL +P +K++  DF T  E + + + E+ +++     
Sbjct: 232 DAETPRLIFERTLFKSDVNSNLSRLLMPFQKLIRNDFLTPAECRAMQKDEDNDEEDDENI 291

Query: 124 GMNVVIVDDIVGERSLCLKKWKI----GSGDV-YCLMTQWNSFVEETGLKSGQHIQLWCF 175
           G+  V+V+    +  L  K W +    G G + Y L   WN  V+ + LK G  I LW F
Sbjct: 292 GVGTVLVNQRFQKWGLRFKIWAMEKDSGHGTLNYTLNWGWNDVVKSSSLKVGDKISLWTF 351

The following BLAST results are available for this feature:

BLAST of HG10009557.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876237.1	1.4e-74	80.57	putative B3 domain-containing protein At3g24850 [Benincasa hispida]	[more]
XP_038905532.1	8.2e-72	78.21	putative B3 domain-containing protein At3g24850 [Benincasa hispida]	[more]
XP_038874960.1	2.6e-70	77.46	B3 domain-containing protein At2g31420-like [Benincasa hispida]	[more]
XP_038874962.1	3.4e-70	76.70	putative B3 domain-containing protein At5g35780 [Benincasa hispida]	[more]
XP_038907053.1	1.4e-68	80.59	B3 domain-containing protein At2g31420-like [Benincasa hispida]	[more]

BLAST of HG10009557.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5RM09	2.0e-12	32.52	B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05...	[more]
Q8RV83	3.2e-10	31.09	B3 domain-containing protein At2g31720 OS=Arabidopsis thaliana OX=3702 GN=ARF70 ...	[more]
Q9XIP5	1.6e-09	32.22	Putative B3 domain-containing protein At2g27410 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SJ98	1.9e-07	25.86	B3 domain-containing protein At2g24670 OS=Arabidopsis thaliana OX=3702 GN=At2g24...	[more]
Q9FFX2	7.3e-07	29.74	B3 domain-containing protein At5g38490 OS=Arabidopsis thaliana OX=3702 GN=At5g38...	[more]

BLAST of HG10009557.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CT22	8.0e-65	73.33	Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CSS4	2.3e-64	72.78	putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7TY94	1.2e-60	72.19	Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S4E600	1.2e-60	72.19	putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7TWX2	3.5e-60	68.93	Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of HG10009557.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05920.1	1.4e-13	32.52	Domain of unknown function (DUF313)	[more]
AT2G31720.1	2.3e-11	31.09	Domain of unknown function (DUF313)	[more]
AT2G27410.1	1.1e-10	32.22	Domain of unknown function (DUF313)	[more]
AT2G24670.1	1.4e-08	25.86	Domain of unknown function (DUF313)	[more]
AT5G38490.1	5.2e-08	29.74	Domain of unknown function (DUF313)	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005508	B3 domain-containing protein At2g31720-like	PFAM	PF03754	DUF313	coord: 38..144 e-value: 3.3E-20 score: 72.4
IPR005508	B3 domain-containing protein At2g31720-like	PANTHER	PTHR31541	B3 DOMAIN PLANT PROTEIN-RELATED	coord: 11..177
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 50..170 e-value: 2.5E-15 score: 58.3
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 58..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..40
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	PANTHER	PTHR31541:SF25	DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATED	coord: 11..177

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
HG10009557	HG10009557	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
HG10009557.1-cds	HG10009557.1-cds-Chr06:7446614..7447144	CDS
HG10009557.1-cds	HG10009557.1-cds-Chr06:7447616..7447621	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
HG10009557.1	HG10009557.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding