HG10009148.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009148.1 vs. NCBI nr
Match: KAA0042770.1 (oxalate--CoA ligase-like [Cucumis melo var. makuwa])

HSP 1 Score: 926.0 bits (2392), Expect = 1.4e-265
Identity = 473/513 (92.20%), Postives = 487/513 (94.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCTL GLLKKAA EFP RRALSVSGKFD+THERLQELIEHAAARLVD+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVK  LSSGDE+IELFPS  E  SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           +TFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANK+AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITDS+PKTATGKIQRR VAEHFLAAKA
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFLAAKA 513

BLAST of HG10009148.1 vs. NCBI nr
Match: XP_004143916.1 (oxalate--CoA ligase [Cucumis sativus] >KGN50110.1 hypothetical protein Csa_000659 [Cucumis sativus])

HSP 1 Score: 923.7 bits (2386), Expect = 6.9e-265
Identity = 473/513 (92.20%), Postives = 487/513 (94.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCT  GLLKKAA EFPR RALSVSGKFD+THERLQELIEHAAARLVDAGV AGDVVAL
Sbjct: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVKA+LSSGDEFIELFPS  ET SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNL ASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDAN +AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL+FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITD +PKTA+GKIQRRFVAEHFLAAKA
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 513

BLAST of HG10009148.1 vs. NCBI nr
Match: XP_008437250.1 (PREDICTED: oxalate--CoA ligase-like [Cucumis melo])

HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 472/513 (92.01%), Postives = 486/513 (94.74%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCTL GLLKKAA EFP RRALSVSGKFD+THERLQELIEHAAARLVD+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TF NTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFLNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVK  LSSGDE+IELFPS  E  SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           +TFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANK+AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITDS+PKTATGKIQRR VAEHFLAAKA
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFLAAKA 513

BLAST of HG10009148.1 vs. NCBI nr
Match: XP_038874771.1 (oxalate--CoA ligase-like [Benincasa hispida])

HSP 1 Score: 915.6 bits (2365), Expect = 1.9e-262
Identity = 467/513 (91.03%), Postives = 487/513 (94.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCTL GLLKKAA EFPRRRALSVSGKFD+THERLQEL+ HAA  LVD GV AGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPRRRALSVSGKFDLTHERLQELVVHAAGCLVDVGVKAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLL+TSK+GIPSAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLITSKEGIPSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVKA+LSSG+EFI+LFPSATETKSN AE VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGNEFIDLFPSATETKSNIAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK SESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKFSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDMIAYNATWYTAVPTIHQIIL+RH SKPE SYPKLRFIRSCSASLAPS+LERLE 
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILDRHFSKPEASYPKLRFIRSCSASLAPSVLERLET 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           S GAPVLE+YAMTEASHLMASNPLPEDG HKAGSVGKPIGQEMAILDENGV+Q EGVKGE
Sbjct: 301 SLGAPVLEAYAMTEASHLMASNPLPEDGGHKAGSVGKPIGQEMAILDENGVMQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRG NVTKGYKNNP+ANKAAFMFGWFHTGD+GFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGANVTKGYKNNPEANKAAFMFGWFHTGDVGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPDEKYGEEINCAIIPR GSSIDEA+VL+FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHANIAQAVAFGVPDEKYGEEINCAIIPRGGSSIDEADVLQFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITD +PKTA+GKIQRRFVAEHFLAAKA
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 513

BLAST of HG10009148.1 vs. NCBI nr
Match: XP_022995200.1 (oxalate--CoA ligase-like [Cucurbita maxima])

HSP 1 Score: 908.7 bits (2347), Expect = 2.3e-260
Identity = 460/513 (89.67%), Postives = 485/513 (94.54%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MEN TL GLLKKAA EFP RRALSV+GK D+THERLQELIEHAA+RLVDAGVNAGDVVAL
Sbjct: 1   MENSTLTGLLKKAASEFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVE+VI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GIPSAQTAA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           SKLN+PQV  +L+SGD F++ FP+ +E+K N AE VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPAPSESKVNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDM+AYNATWYTAVPTIHQIIL+RH SKPEPSYPKLRFIRSCSASLAPSILERLEK
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGV+Q EGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANKA F FGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIE+DAVLLSH     A  FGV DEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK
Sbjct: 421 PIEIDAVLLSHADIAQAVAFGVTDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           +PKKVFITD +PKTA+GKIQRRFVAEHF++AKA
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVSAKA 513

BLAST of HG10009148.1 vs. ExPASy Swiss-Prot
Match: M4IQQ7 (Probable CoA ligase CCL9 OS=Humulus lupulus OX=3486 GN=CCL9 PE=2 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.1e-228
Identity = 403/510 (79.02%), Postives = 443/510 (86.86%), Query Frame = 0

Query: 3   NCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVALTF 62
           N TL GLLKKAA EF  RRA+ VSG+F +TH RLQE+++HAA+ L+ +GV  GDVVALTF
Sbjct: 4   NITLTGLLKKAAAEFSDRRAILVSGEFHLTHARLQEIVDHAASLLLASGVGHGDVVALTF 63

Query: 63  PNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAASK 122
           PNT+EY++MFLAVIRCRA AAPLN AYTAEEFEFYLSDSESKLL+T   GI +A+ AASK
Sbjct: 64  PNTIEYIVMFLAVIRCRAVAAPLNSAYTAEEFEFYLSDSESKLLITPPKGIEAARAAASK 123

Query: 123 LNLPQVKADLSSGDEFIELFPSATETKS--NTAESVNDPADAALFLHTSGTTSRPKGVPL 182
           LN+  V A L  GD  I L PS     S    AE  NDP+D +LFLHTSGTTSRPKGVPL
Sbjct: 124 LNITHVTATLPGGDGLIALSPSPNNESSLDAVAELTNDPSDVSLFLHTSGTTSRPKGVPL 183

Query: 183 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 242
           TQ NLA+SVQNIKSVYKLSESDSTV+VLPLFHVHG++AGLLSSLIAG+AVTLPAAGRFSA
Sbjct: 184 TQLNLASSVQNIKSVYKLSESDSTVVVLPLFHVHGMIAGLLSSLIAGSAVTLPAAGRFSA 243

Query: 243 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 302
           STFWSDMIA NATWYTAVPTIHQIIL+RH +KPEP+YPKLRFIRSCSASLAP+I+ RLE+
Sbjct: 244 STFWSDMIACNATWYTAVPTIHQIILDRHLNKPEPTYPKLRFIRSCSASLAPTIMGRLEE 303

Query: 303 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 362
           SFGAPVLE+YAMTEA+HLMASNPLPEDG HK GSVGKP+GQEMAILD NG  Q  G  GE
Sbjct: 304 SFGAPVLEAYAMTEAAHLMASNPLPEDGGHKPGSVGKPVGQEMAILDLNGSAQLPGFSGE 363

Query: 363 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 422
           VCIRGPNVTKGYKNNP+ANKAAF FGWFHTGD+G+FD DGYLHLVGRIKELINRGGEKIS
Sbjct: 364 VCIRGPNVTKGYKNNPEANKAAFQFGWFHTGDVGYFDEDGYLHLVGRIKELINRGGEKIS 423

Query: 423 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 482
           PIEVDAVLLSH     A  FGVPD+KYGEEINCA+IPREGS +DE  VLRFCKKNLA+FK
Sbjct: 424 PIEVDAVLLSHPDLAQAVAFGVPDDKYGEEINCAVIPREGSEVDEDGVLRFCKKNLAAFK 483

Query: 483 VPKKVFITDSVPKTATGKIQRRFVAEHFLA 506
           VPKKVFITDS+PKTATGKIQRR VAEHFLA
Sbjct: 484 VPKKVFITDSLPKTATGKIQRRIVAEHFLA 513

BLAST of HG10009148.1 vs. ExPASy Swiss-Prot
Match: Q9SMT7 (Oxalate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE3 PE=1 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.0e-215
Identity = 383/511 (74.95%), Postives = 434/511 (84.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLV-DAGVNAGDVVA 60
           M++ TL GLL+  A +FP RRALSVSGKF++TH RL +LIE AA+RLV DAG+  GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTA 120
           LTFPNTVE+VIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SKLLLTSK+G   AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ASKLNLPQVKAD-LSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGV 180
           ASKL +  V A  L +G + +     +     +  E VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRF 240
           PLTQ NLA+SV+NIK+VYKL+ESDSTV+VLPLFHVHGL+AGLLSSL AGAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERL 300
           SA+TFW DM  YNATWYTAVPTIHQIIL+RH S PE  YPKLRFIRSCSASLAP IL RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 EKSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVK 360
           E++FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKP+GQEMAIL+E G +Q    K
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNP+ANKA F FGWFHTGDIG+FD+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLAS 480
           ISPIEVDAVLL+H        FGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKKVFITDSVPKTATGKIQRRFVAEHFL 505
           FKVPK+VFITD++PKTA+GKIQRR VA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of HG10009148.1 vs. ExPASy Swiss-Prot
Match: O74976 (Oxalate--CoA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pcs60 PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.2e-117
Identity = 233/497 (46.88%), Postives = 314/497 (63.18%), Query Frame = 0

Query: 20  RRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVALTFPNTVEYVIMFLAVIRCR 79
           R  ++ S   +++   L+  I     ++   G+  GD V +  PN +E+V+ F AV   R
Sbjct: 17  RALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQR 76

Query: 80  ATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPS---AQTAASKLNLPQVKADLSSGD 139
           A   PLN  Y   EFEFY+ D +SKL++  +  + +   A  AA KL++   +       
Sbjct: 77  AICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWCPKS 136

Query: 140 EFIEL--FPSATETKSNTAESVN--DPADAALFLHTSGTTSRPKGVPLTQQNLAASVQNI 199
             + +  F  A   K N  + +    P D  L LHTSGTT RPK VPLT +NL  S+ NI
Sbjct: 137 RLVRIVHFEGA---KINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNLCRSIHNI 196

Query: 200 KSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNA 259
            + Y+L   D++ +V+PLFHVHGL+ GLLS+L +G    +P   +FSA +FW + I Y A
Sbjct: 197 TTSYRLDPRDTSYVVMPLFHVHGLLCGLLSTLASGGCAVVPP--KFSAHSFWKEFIQYGA 256

Query: 260 TWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEKSFGAPVLESYAM 319
           TWYTAVPTIHQI+L     KP    P++RFIRSCS+ LAP +L +LE +F APVLE+YAM
Sbjct: 257 TWYTAVPTIHQILLRTPPPKP---LPRIRFIRSCSSPLAPPVLSKLEATFRAPVLEAYAM 316

Query: 320 TEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGEVCIRGPNVTKGY 379
           TEASH M +NPLP   VHK  SVGKP G E+ ILD+ G    +G +GE+C+RG NVTKGY
Sbjct: 317 TEASHQMTTNPLP-PLVHKPHSVGKPFGVELKILDQKGNEMPQGKEGEICVRGINVTKGY 376

Query: 380 KNNPDANKAAFMFG-WFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSH 439
            NNP ANK++F    +F TGD G  D DGY+ + GRIKEL+NRGGEKISP E+DAVL+ H
Sbjct: 377 LNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDAVLMQH 436

Query: 440 -----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFKVPKKVFITDSV 499
                A  F VPDEKYG++I  AI P  G ++   ++  + ++ +A+FK+PKK + TD +
Sbjct: 437 PDVSEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQLHDYLEQKVAAFKIPKKFYFTDRI 496

Query: 500 PKTATGKIQRRFVAEHF 504
           PKTATGK+QRR V + F
Sbjct: 497 PKTATGKVQRRLVCDAF 504

BLAST of HG10009148.1 vs. ExPASy Swiss-Prot
Match: P38137 (Oxalate--CoA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PCS60 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 4.1e-103
Identity = 225/508 (44.29%), Postives = 298/508 (58.66%), Query Frame = 0

Query: 31  ITHERLQELIEHAAARLVD------AGVNAGDVVALTFPNTVEYVIMFLAVIRCRATAAP 90
           +T+  L  ++ H      +        V   D VA++  N +E+++ FL         AP
Sbjct: 32  VTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAP 91

Query: 91  LNPAYTAEEFEFYLSDSESKLL--------LTSKDGIPSAQT-------AASKLNLPQVK 150
           LNP Y  +EF FYL+D +SK +        L S + + SA T        A      +V+
Sbjct: 92  LNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVE 151

Query: 151 ADLSSGDEFIE--LFPSATETKSNTAESVNDP-----ADAALFLHTSGTTSRPKGVPLTQ 210
            D+ S ++  +  ++ S    K      V  P     +D AL LHTSGTTS PK VPL  
Sbjct: 152 YDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLH 211

Query: 211 QNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSAST 270
            N+  S  NI + YKL+  D + +V+PLFHVHGL+  LLS+     +V +P    F    
Sbjct: 212 LNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDG--FHPKL 271

Query: 271 FWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEKSF 330
           FW   + YN  W++ VPTI  I+L  +  KP P +P +RFIRSCS++LAP+   +LEK F
Sbjct: 272 FWDQFVKYNCNWFSCVPTISMIML--NMPKPNP-FPHIRFIRSCSSALAPATFHKLEKEF 331

Query: 331 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGEVC 390
            APVLE+YAMTEASH M SN LP  G  K G+VG+P G  + ILD+N  V   G  GEV 
Sbjct: 332 NAPVLEAYAMTEASHQMTSNNLP-PGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVS 391

Query: 391 IRGPNVTKGYKNNPDANKAAF--MFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 450
           IRG NVT GY NNP ANK  F     +F TGD G+FD +G+L L GRIKELINRGGEKIS
Sbjct: 392 IRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKIS 451

Query: 451 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 504
           PIE+D ++LSH     A  FGVPD+ YG+ +  AI+ ++G  +   E++ F KK+LASFK
Sbjct: 452 PIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFK 511

BLAST of HG10009148.1 vs. ExPASy Swiss-Prot
Match: Q8J2R0 (Acyl-CoA synthetase FUM10 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FUM10 PE=3 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 2.0e-65
Identity = 177/525 (33.71%), Postives = 260/525 (49.52%), Query Frame = 0

Query: 31  ITHERLQELIEHAAARLVDAGVNAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNPAYT 90
           + +  L +L +     L   G++ G  VA+  PN + +V + LA IR RA +AP++P  T
Sbjct: 35  VPYAHLTDLTQSLQHDLATLGISVGSKVAIVLPNGLAFVTVLLATIRQRAISAPIHPNST 94

Query: 91  AEEFE--FYLSDSESKLLLTSK-DGIPSAQTAASKLNLPQVKAD---------------- 150
            EE +  F L   +   ++ S+     +A  AA  L LP                     
Sbjct: 95  REECKQIFSLMTPDLVAVMPSEASPAAAAVLAAQDLGLPVASCHRYPQSGRLSFCLALKL 154

Query: 151 LSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPLTQQNLAASVQN 210
           ++ GDE     P    ++ +         D  L L TSGTT  PK V LT  N+  +++ 
Sbjct: 155 VNRGDETSNPSPCMVYSRDHVLAE-----DKVLELFTSGTTGAPKSVQLTHTNILVAMRI 214

Query: 211 IKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYN 270
           I S +K++  D + L+ PLFH+ G+   LL +L  G    +PA+     +TFW D   Y+
Sbjct: 215 ITSAHKITFKDRSFLITPLFHIIGIAGSLLPTLFTGGCAVIPAS---LPATFWQDCQDYS 274

Query: 271 ATWYTAVPTIHQIILERHCSKPEPSYP-KLRFIRSCSASLAPSILERLEKSFGAPVLESY 330
            TWY AVPT+H ++L      P+   P  LRFIRS  + ++  +  RL+K  G P+LE Y
Sbjct: 275 ITWYHAVPTLHHLLLS--FPMPKGGVPATLRFIRSGGSDMSLDLFNRLQK-LGVPLLEVY 334

Query: 331 AMTEASHLMASNPLPEDGVHKA-----GSVGKPIGQEMAIL------------DENGVVQ 390
            MTE +  +  NP P      A     G    P   ++ IL            D +G+  
Sbjct: 335 GMTETAPAIFCNPFPVTETSTAIKRHPGQYPIPDAVDVMILPPERAPGLEPNGDLDGIEN 394

Query: 391 SE---------GVKGEVCIRGPNVTKGYKNNPDANKAAFM-FGWFHTGDIGFFDSDGYLH 450
           +E         GVKGE+C+RG N+  GY NNP AN+ AF+  G+F TGD+G      YL 
Sbjct: 395 TEPVARLTKELGVKGEICLRGKNIMAGYTNNPAANREAFLPNGFFRTGDLGVIKPRQYLA 454

Query: 451 LVGRIKELINRGGEKISPIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSI 504
           L+GR+KE+IN+GGEKISP E++ V   H     A  F + DE YGE I  AI  +  S+I
Sbjct: 455 LIGRVKEIINKGGEKISPAEIEHVARLHDQVNDAACFRISDEIYGEVIGLAITAK--SAI 514

BLAST of HG10009148.1 vs. ExPASy TrEMBL
Match: A0A5A7THK1 (Oxalate--CoA ligase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002700 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.7e-266
Identity = 473/513 (92.20%), Postives = 487/513 (94.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCTL GLLKKAA EFP RRALSVSGKFD+THERLQELIEHAAARLVD+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVK  LSSGDE+IELFPS  E  SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           +TFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANK+AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITDS+PKTATGKIQRR VAEHFLAAKA
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFLAAKA 513

BLAST of HG10009148.1 vs. ExPASy TrEMBL
Match: A0A0A0KKA0 (AMP dependent CoA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G154230 PE=4 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.3e-265
Identity = 473/513 (92.20%), Postives = 487/513 (94.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCT  GLLKKAA EFPR RALSVSGKFD+THERLQELIEHAAARLVDAGV AGDVVAL
Sbjct: 1   MENCTFTGLLKKAASEFPRCRALSVSGKFDLTHERLQELIEHAAARLVDAGVKAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVKA+LSSGDEFIELFPS  ET SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGDEFIELFPSPIETGSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNL ASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLGASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILERHLSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDAN +AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANNSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL+FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHTDVSQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLQFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITD +PKTA+GKIQRRFVAEHFLAAKA
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFLAAKA 513

BLAST of HG10009148.1 vs. ExPASy TrEMBL
Match: A0A1S3AT78 (oxalate--CoA ligase-like OS=Cucumis melo OX=3656 GN=LOC103482735 PE=4 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 9.7e-265
Identity = 472/513 (92.01%), Postives = 486/513 (94.74%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MENCTL GLLKKAA EFP RRALSVSGKFD+THERLQELIEHAAARLVD+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TF NTVEYVIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GI SAQTAA
Sbjct: 61  TFLNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           +KLN+PQVK  LSSGDE+IELFPS  E  SN  E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           +TFWSDMIAYNATWYTAVPTIHQIILERH SKPEPSYPKLRFIRSCSASLAPSILERLE+
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENG +QSEGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANK+AFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPD KYGEEINCAIIPREGSSI+EA+VL FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           VPKKVFITDS+PKTATGKIQRR VAEHFLAAKA
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFLAAKA 513

BLAST of HG10009148.1 vs. ExPASy TrEMBL
Match: A0A6J1K3G7 (oxalate--CoA ligase-like OS=Cucurbita maxima OX=3661 GN=LOC111490818 PE=4 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.1e-260
Identity = 460/513 (89.67%), Postives = 485/513 (94.54%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MEN TL GLLKKAA EFP RRALSV+GK D+THERLQELIEHAA+RLVDAGVNAGDVVAL
Sbjct: 1   MENSTLTGLLKKAASEFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVAL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVE+VI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GIPSAQTAA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           SKLN+PQV  +L+SGD F++ FP+ +E+K N AE VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPAPSESKVNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDM+AYNATWYTAVPTIHQIIL+RH SKPEPSYPKLRFIRSCSASLAPSILERLEK
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGV+Q EGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPDANKA F FGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIE+DAVLLSH     A  FGV DEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK
Sbjct: 421 PIEIDAVLLSHADIAQAVAFGVTDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           +PKKVFITD +PKTA+GKIQRRFVAEHF++AKA
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVSAKA 513

BLAST of HG10009148.1 vs. ExPASy TrEMBL
Match: A0A6J1H1B7 (oxalate--CoA ligase-like OS=Cucurbita moschata OX=3662 GN=LOC111459244 PE=4 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.2e-260
Identity = 458/513 (89.28%), Postives = 484/513 (94.35%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLVDAGVNAGDVVAL 60
           MEN TL GLLKKAA +FP RRALSV+GK D+THERLQELIEHAA+RLVDAGVNAGDVV L
Sbjct: 1   MENSTLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVTL 60

Query: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAA 120
           TFPNTVE+VI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTSK+GIPSAQTAA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 SKLNLPQVKADLSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGVPL 180
           SKLN+PQV  +L+SGD F++ FPS  E+K N +E VNDP+DAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERLEK 300
           STFWSDM+AYNATWYTAVPTIHQIIL+RH SKPEPSYPKLRFIRSCSASLAPSILERLEK
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVKGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGV+Q EGVKGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNP+ANKA F FGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480
           PIEVDAVLLSH     A  FGVPDEKYGEEINCA+IPREGSSIDEAEVLRFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHSDIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKKVFITDSVPKTATGKIQRRFVAEHFLAAKA 509
           +PKKVFITD +PKTA+GKIQRRFVAEHF++AKA
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVSAKA 513

BLAST of HG10009148.1 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 750.7 bits (1937), Expect = 7.4e-217
Identity = 383/511 (74.95%), Postives = 434/511 (84.93%), Query Frame = 0

Query: 1   MENCTLIGLLKKAAFEFPRRRALSVSGKFDITHERLQELIEHAAARLV-DAGVNAGDVVA 60
           M++ TL GLL+  A +FP RRALSVSGKF++TH RL +LIE AA+RLV DAG+  GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTA 120
           LTFPNTVE+VIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SKLLLTSK+G   AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ASKLNLPQVKAD-LSSGDEFIELFPSATETKSNTAESVNDPADAALFLHTSGTTSRPKGV 180
           ASKL +  V A  L +G + +     +     +  E VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRF 240
           PLTQ NLA+SV+NIK+VYKL+ESDSTV+VLPLFHVHGL+AGLLSSL AGAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSCSASLAPSILERL 300
           SA+TFW DM  YNATWYTAVPTIHQIIL+RH S PE  YPKLRFIRSCSASLAP IL RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 EKSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVVQSEGVK 360
           E++FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKP+GQEMAIL+E G +Q    K
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPDANKAAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNP+ANKA F FGWFHTGDIG+FD+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLAS 480
           ISPIEVDAVLL+H        FGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKKVFITDSVPKTATGKIQRRFVAEHFL 505
           FKVPK+VFITD++PKTA+GKIQRR VA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of HG10009148.1 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 190.7 bits (483), Expect = 3.0e-48
Identity = 151/520 (29.04%), Postives = 241/520 (46.35%), Query Frame = 0

Query: 3   NCTLIGLLKKAAFEFPRRRALSVSGKFD-ITHERLQELIEHAAARLVDAGVNAGDVVALT 62
           N +L+  L + +  +P + A++ S   D +T  +L+  +   A      G+   DVV + 
Sbjct: 26  NTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIF 85

Query: 63  FPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAAS 122
            PN+ ++ + FLAV          NP YT  E    + DS  K+++       S      
Sbjct: 86  APNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIII-------SVNQLFD 145

Query: 123 KLNLPQVKADLSSGDEFIELFPSAT------ETKSNTAESVND-------PADAALFLHT 182
           K+    +   L    + +E+ P +       +     +E V++        +D A  L++
Sbjct: 146 KIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYS 205

Query: 183 SGTTSRPKGVPLTQQN-LAASVQNIKSVYKLSESDSTVL-VLPLFHVHGLMAGLLSSLIA 242
           SGTT   KGV LT  N +AAS+        + E     L  LP+FHV GL     S L  
Sbjct: 206 SGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQR 265

Query: 243 GAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQIILERHCSKPEPSYPKLRFIRSC 302
           G A  L +  RF       ++  +  T    VP +  + L +     +     L++I S 
Sbjct: 266 GNA--LVSMARFELELVLKNIEKFRVTHLWVVPPVF-LALSKQSIVKKFDLSSLKYIGSG 325

Query: 303 SASLAPSILERLEKSF-GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVG--KPIGQEM 362
           +A L   ++E   ++     +++ Y MTE   +++    P  G   +GS G   P  +  
Sbjct: 326 AAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVED-PRLGKRNSGSAGMLAPGVEAQ 385

Query: 363 AILDENGVVQSEGVKGEVCIRGPNVTKGYKNNPDANKAAF-MFGWFHTGDIGFFDSDGYL 422
            +  E G  Q    +GE+ +RGPN+ KGY NNP A K       W HTGD+G+F+ DG L
Sbjct: 386 IVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNL 445

Query: 423 HLVGRIKELINRGGEKISPIEVDAVLLSH-----ACFFGVPDEKYGEEINCAIIPREGSS 482
           ++V RIKELI   G +++P E++ +L+SH     A     PDE+ GE     ++    SS
Sbjct: 446 YVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSS 505

Query: 483 IDEAEVLRFCKKNLASFKVPKKVFITDSVPKTATGKIQRR 498
           I E ++ +F  K +A +K  ++V     VPK+A GKI RR
Sbjct: 506 ITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRR 534

BLAST of HG10009148.1 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.9e-47
Identity = 142/485 (29.28%), Postives = 243/485 (50.10%), Query Frame = 0

Query: 29  FDITHERLQELIEHAAARLVDA-GVNAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNP 88
           F I+H  LQ +++  AA +    GV  GDVV+L  PN+V + ++FL++I   A    +NP
Sbjct: 71  FSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNP 130

Query: 89  AYTAEEFEFYLSDSESKLLLTSKDGIPSAQTAASKLNLPQVKADLSS-GDEFIELFPSAT 148
           + +  E +  +S+    L  TS + +    +    +       D  S   E  + +    
Sbjct: 131 SSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMK 190

Query: 149 ETKSNTAESVNDPADAALFLHTSGTTSRPKGVPLTQQNLAASVQNI----KSVYKLSESD 208
           E+     + +    D A  +++SGTT   KGV LT +NL AS++       S Y+   S 
Sbjct: 191 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 250

Query: 209 STVL-VLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTI 268
           +  L  LPL H++GL   ++  L  G+  T+    RF AS   + +  +  T +  VP +
Sbjct: 251 NVYLAALPLCHIYGLSLFVMGLLSLGS--TIVVMKRFDASDVVNVIERFKITHFPVVPPM 310

Query: 269 HQIILERHCSKPEPSYPKLRFIRSCSASLAPSILER-LEKSFGAPVLESYAMTEASHLMA 328
              + ++        +  L+ + S +A L+   +E  L+      +++ Y MTE S  + 
Sbjct: 311 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTE-STAVG 370

Query: 329 SNPLPEDGVHKAGSVG--KPIGQEMAILDENGVVQSEGVKGEVCIRGPNVTKGYKNNPDA 388
           +     + + +  SVG   P  Q   +   +G     G +GE+ I+GP V KGY NNP A
Sbjct: 371 TRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKA 430

Query: 389 NKAAFM-FGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSH-----A 448
            + + +   W  TGDI +FD DGYL +V RIKE+I   G +I+P +++AVL+SH     A
Sbjct: 431 TQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDA 490

Query: 449 CFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFKVPKKVFITDSVPKTATG 498
                P+E+ GE     ++ R+ +++ E +V+ +    +A ++  +KV + +S+PK+ TG
Sbjct: 491 AVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTG 550

BLAST of HG10009148.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-46
Identity = 155/495 (31.31%), Postives = 230/495 (46.46%), Query Frame = 0

Query: 32  THERLQELIEHAAARLVDAGVNAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNPAYTA 91
           T+  +  +    AA     GVN  DVV L  PN  E+V+ FLA     ATA   NP +T 
Sbjct: 67  TYSDVHVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTP 126

Query: 92  EEFEFYLSDSESKLLLTSKDGIPSAQTAASKLNLPQVKADLSSGD-------EFIELFPS 151
            E       S +KL++T    +   +   +   +  V  D +           F EL  S
Sbjct: 127 AEIAKQAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQS 186

Query: 152 ATETKSNTAESVN-DPADAALFLHTSGTTSRPKGVPLTQQNLAASV-QNI--KSVYKLSE 211
            TE  S   +SV   P D     ++SGTT  PKGV LT + L  SV Q +  ++      
Sbjct: 187 TTEA-SEVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFH 246

Query: 212 SDSTVL-VLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVP 271
           SD  +L VLP+FH++ L + +L  L  GAA+ +    +F  +     +        T  P
Sbjct: 247 SDDVILCVLPMFHIYALNSIMLCGLRVGAAILI--MPKFEINLL---LELIQRCKVTVAP 306

Query: 272 TIHQIILERHCSKPEPSY--PKLRFIRSCSASLAPSILERLEKSF-GAPVLESYAMTEAS 331
            +  I+L    S     Y    +R ++S +A L   + + +   F  A + + Y MTEA 
Sbjct: 307 MVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAG 366

Query: 332 HLM------ASNPLPEDGVHKAGSVGKPI-GQEMAILD-ENGVVQSEGVKGEVCIRGPNV 391
            ++      A  P P     K+G+ G  +   EM I+D + G   S    GE+CIRG  +
Sbjct: 367 PVLAMSLGFAKEPFPV----KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQI 426

Query: 392 TKGYKNNPDANKAAF-MFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAV 451
            KGY NNP A        GW HTGDIG  D D  L +V R+KELI   G +++P E++A+
Sbjct: 427 MKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 486

Query: 452 LLSH-----ACFFGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFKVPKKVFI 498
           L+ H          + +E  GE     ++  + S + E +V +F  K +  +K   KVF 
Sbjct: 487 LIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFF 546

BLAST of HG10009148.1 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 183.7 bits (465), Expect = 3.6e-46
Identity = 139/483 (28.78%), Postives = 232/483 (48.03%), Query Frame = 0

Query: 30  DITHERLQELIEHAAARLVDAGVNAGDVVALTFPNTVEYVIMFLAVIRCRATAAPLNPAY 89
           ++T   L   +E  A  L + G+  G VV L  PN++ + ++ L+V+   A     NP  
Sbjct: 57  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 116

Query: 90  TAEEFEFYLSDSESKLLLTSKDGIPSAQTAASKLNLPQVKADL--SSGD--EFIELFPSA 149
           T+ E    + DS   L  T+   +P    AA KL +  +  +   S GD    +E+    
Sbjct: 117 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK- 176

Query: 150 TETKSNTAESVNDPADAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKLSESDST- 209
            E   N  +   D  D A  L++SGTT   KGV  + +NL A VQ I + +   + +   
Sbjct: 177 -EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRF 236

Query: 210 VLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFWSDMIAYNATWYTAVPTIHQI 269
           +  +P+FH++GL A     L  G+  T+    +F      S +  Y AT    VP I   
Sbjct: 237 ICTVPMFHIYGLAAFATGLLAYGS--TIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVA 296

Query: 270 ILE-RHCSKPEPSYPKLRFIRSCSASLAPSILERL-EKSFGAPVLESYAMTEASHLMASN 329
           ++      K +     +  +    A L+  + E   EK     +L+ Y +TE++ + AS 
Sbjct: 297 MVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGAST 356

Query: 330 PLPEDGVHKAGSVGK-PIGQEMAILDE-NGVVQSEGVKGEVCIRGPNVTKGYKNNPDANK 389
              E+   + G+ GK     E  I+D   G +      GE+ ++GP++ KGY +N +A  
Sbjct: 357 DTVEES-RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATS 416

Query: 390 AAF-MFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSH-----ACF 449
           +     GW  TGD+ + D DG++ +V R+KELI   G +++P E++A+LL+H     A  
Sbjct: 417 STLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAV 476

Query: 450 FGVPDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFKVPKKVFITDSVPKTATGKI 498
              PD++ G+     ++ + GSS+ E  ++ F  K +A +K  +KV    S+PK  +GKI
Sbjct: 477 IPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKI 534