HG10008983.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10008983.1 vs. NCBI nr
Match: KAG6579613.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 593.2 bits (1528), Expect = 1.5e-165
Identity = 303/350 (86.57%), Postives = 329/350 (94.00%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA VIDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI TAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IRTARGAAK++GTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIYKN DS NSSGK+
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+CLFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQNTS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 350

BLAST of HG10008983.1 vs. NCBI nr
Match: XP_022970063.1 (WAT1-related protein At1g43650-like [Cucurbita maxima])

HSP 1 Score: 591.3 bits (1523), Expect = 5.8e-165
Identity = 302/350 (86.29%), Postives = 328/350 (93.71%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA VIDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI TAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IRTARGAAK+ GTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIY+N DS NSSGK+
Sbjct: 121 EKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+CLFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQNTS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 350

BLAST of HG10008983.1 vs. NCBI nr
Match: XP_022928958.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 591.3 bits (1523), Expect = 5.8e-165
Identity = 302/350 (86.29%), Postives = 329/350 (94.00%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA VIDRN
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI TAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IRTARGAAK++GTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIYKN DS NSSGK+
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+CLFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQ+TS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTS 350

BLAST of HG10008983.1 vs. NCBI nr
Match: XP_023521473.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585.9 bits (1509), Expect = 2.4e-163
Identity = 299/350 (85.43%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMV VQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA  IDRN
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYFIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTIPTAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTIPTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IR+A G AK+LGTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIYKN DS NSSGK+
Sbjct: 121 EKVNIRSAGGGAKVLGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+C FATAQSGFLALIFAR P K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICFFATAQSGFLALIFARDPQK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQNTS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 350

BLAST of HG10008983.1 vs. NCBI nr
Match: XP_023520560.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 7.1e-163
Identity = 297/350 (84.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA  IDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYFIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTIPTAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTIPTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IR+A G AK+LGTI+CIGGSL+FTLWKGPYVTK +FKEPLIDIYKN DS NSSGK+
Sbjct: 121 EKVNIRSAGGGAKVLGTIVCIGGSLIFTLWKGPYVTKGMFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+C FATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICFFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISHNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQNTS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 350

BLAST of HG10008983.1 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.4e-60
Identity = 131/324 (40.43%), Postives = 203/324 (62.65%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           AMV VQI Y G  +L K+A  +  +PFVFV YR   A L L+PFA  ++ +K S L+   
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 70  AIKIFLLAISG-SIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            +KIF +++ G ++ LN++Y  I  T+ T A+A +N IPS+TF+LALLFRLE V ++ + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNS---SGKNWIKGSA 189
           G AK+ G+++ + G+LVF   KGP          LI+ Y +    N    S KN +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 190 FILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWD 249
            +L ++    +W+I+Q+ + ++YPA+L +  L CLF+  QS   A+   R+P  WK+++ 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 250 ARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGS 309
             LL++ Y GI+++G+ Y+LQ W I   GPVFT++++PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 310 LVGAFFIILGLYLVLWGKQADHMV 330
           + GA  ++ GLYL LWGK  +  +
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of HG10008983.1 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.0e-59
Identity = 125/356 (35.11%), Postives = 211/356 (59.27%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           AM+ +Q GY G NI+ KI+    +S +V VVYR+ IA  V+APFA   +R  +  +T   
Sbjct: 21  AMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSI 80

Query: 70  AIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            +++F+L + G  I  N +Y G+ YTSPT + A SN++P++TF+LA+LFR+E + ++   
Sbjct: 81  FMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLW 140

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYV----TKAIFKEPLIDIYKNQDSVNSSGKNWIKGS 189
             AK+ GT++ + G+++ T++KGP V    TK +  +            +SS K ++KGS
Sbjct: 141 CQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGS 200

Query: 190 AFILISDIASSVWLILQAFITRKYPA-QLTITVLMCLFATAQSGFLALIFARSPDKWKLQ 249
             ++ + +A +   +LQA I + Y   QL++T L+C   T Q+  +  +   +P  W++ 
Sbjct: 201 ILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIG 260

Query: 250 WDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHV 309
           WD  LL   Y GIV S + Y++Q   +   GPVF + FSPLM++ VA+  +FV +E++ +
Sbjct: 261 WDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFL 320

Query: 310 GSLVGAFFIILGLYLVLWGKQADHMV--AAEPHENENALVSIEMQNTSGKEPLGQG 358
           G ++GA  I++GLY VLWGKQ ++ V        + N+ V+ +++    K  + +G
Sbjct: 321 GGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKISEG 376

BLAST of HG10008983.1 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.6e-59
Identity = 124/340 (36.47%), Postives = 198/340 (58.24%), Query Frame = 0

Query: 11  MVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPTA 70
           M+ +Q G  G+ I++     +  + +V +VYRN +A LVLAPFAL+ +R  R  +T+   
Sbjct: 17  MIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRPKMTLSVL 76

Query: 71  IKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTARG 130
            KI  L  +   +     Y G+  TS T  SA  NI+PS+TF++A + R+EKV I   R 
Sbjct: 77  WKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKVNIAEVRS 136

Query: 131 AAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKNWIKGSAFILI 190
            AK++GT++ +GG+LV TL+KGP +    +  P +D      + +    NW+ G+  IL+
Sbjct: 137 KAKIIGTLVGLGGALVMTLYKGPLIPLP-WSNPNMDQQNGHTNNSQDHNNWVVGTLLILL 196

Query: 191 SDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWDARLL 250
             +A S + +LQ+   + YPA L+++ L+CL    QS  +AL+  R P  W + WDARL 
Sbjct: 197 GCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVGWDARLF 256

Query: 251 TIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGSLVGA 310
             +Y GIV SG+ Y++Q   +   GPVF + F+PL +I VA+ ++F+  E++H G ++G 
Sbjct: 257 APLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHFGCVIGG 316

Query: 311 FFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
             I  GLY+V+WGK  D+ V+      +N+L  + +   S
Sbjct: 317 AVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKS 355

BLAST of HG10008983.1 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-58
Identity = 117/345 (33.91%), Postives = 209/345 (60.58%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           A++ +Q GY G  I+  ++ +  ++ ++   YR+ +A +V+APFAL+++R  R  +T P 
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKMTWPL 73

Query: 70  AIKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            ++I  L  +   +  N++Y G+  TS T +SAF N +P++TF++A++FR+E V ++  R
Sbjct: 74  FLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTR 133

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNS--SGKNWIKGSAF 189
             AK++GT + +GG++V TL+KGP +   +FK     ++      +S  + +NW+ G+  
Sbjct: 134 SLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSGTSSETTDQNWVTGTLA 193

Query: 190 ILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWDA 249
           ++ S    + + ILQ+F  +KYPA+L++ + +C   T  +   +LI  R    WK+  D+
Sbjct: 194 VMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDS 253

Query: 250 RLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGSL 309
             L  +Y G+V SG+ Y++Q+  I   GPVFT+ FSP+ +I  A     V +E++H+GS+
Sbjct: 254 GTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSI 313

Query: 310 VGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTSGK 352
           +GA FI+ GLY V+WGK  D +++    E +  +  + + NTS K
Sbjct: 314 IGAIFIVFGLYSVVWGKAKDEVISV---EEKIGMQELPITNTSTK 353

BLAST of HG10008983.1 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.4e-58
Identity = 132/363 (36.36%), Postives = 218/363 (60.06%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           +MVV+Q+G  G +IL K    K +S +V VVYR+ +A +V+APFA   D+  R  +T+  
Sbjct: 18  SMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMI 77

Query: 70  AIKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
             KI LL  +   I  N++Y G+ YT+ T A+A  N++P++TF+LA +F LE+VK+R  R
Sbjct: 78  FFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIR 137

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIY--KNQDSVNSSG---KNWIKG 189
              K++GT+  +GG+++ TL KG          P++D++  K   + N++G    + IKG
Sbjct: 138 STGKVVGTLATVGGAMIMTLVKG----------PVLDLFWTKGVSAHNTAGTDIHSAIKG 197

Query: 190 SAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFAR-SPDKWKL 249
           +  + I   + + ++ILQA   R YPA+L++T  +CL  T +   +AL+  + +P  W +
Sbjct: 198 AVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAI 257

Query: 250 QWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLH 309
            WD +LLT  Y GIV S + Y++    +   GPVF + FSPL +I VAI S  +F+E+++
Sbjct: 258 GWDTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMY 317

Query: 310 VGSLVGAFFIILGLYLVLWGKQADHMVAAEPH-ENENALVSIEMQNTSGKEPLGQGVELF 365
           +G ++GA  I  GLYLV+WGK  D+   +    ++E+A   +E+   +GK+ +   V   
Sbjct: 318 LGRVLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSG-NGKDNVDHEVITI 369

BLAST of HG10008983.1 vs. ExPASy TrEMBL
Match: A0A6J1I2R9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.8e-165
Identity = 302/350 (86.29%), Postives = 328/350 (93.71%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA VIDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI TAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IRTARGAAK+ GTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIY+N DS NSSGK+
Sbjct: 121 EKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+CLFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQNTS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTS 350

BLAST of HG10008983.1 vs. ExPASy TrEMBL
Match: A0A6J1EQL7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.8e-165
Identity = 302/350 (86.29%), Postives = 329/350 (94.00%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMVVVQIGYGGSNILMKIA++KDL+PFVFVVYR+FIAFL+LAPFA VIDRN
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI TAIKIFLLAI GS IHLN+FY GIGYTSPTVASAFSN+IPSLTF++A+LFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV IRTARGAAK++GTILCIGGSL+FTLWKGPYVTKAIFKEPLIDIYKN DS NSSGK+
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIA S+WLILQAF+TRKYPAQLT+TVL+CLFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GIVISGVGYFLQTWCIS NGPVFTSMFSPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VAAEP E +   V  EMQ+TS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTS 350

BLAST of HG10008983.1 vs. ExPASy TrEMBL
Match: A0A6J1HY24 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 8.2e-149
Identity = 271/350 (77.43%), Postives = 310/350 (88.57%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMV VQIGYGGSNILMK+A++KDL PFVF VYR+ IAFLVL PFA VIDRN
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K++SLTI T IKIFLLAI GS  +LN+FY GI YTS TVASAFSN+ PS TF++A+LFRL
Sbjct: 61  KQTSLTISTTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV I+TARGAAK+LGT++CIGGSL+FT WKGPY+TKAIFKEPLI+IYKNQDS NSSGK+
Sbjct: 121 EKVNIKTARGAAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFI++S+++ S WLILQ F+TRKYPA+LTITVL C+FATAQSGFLAL FAR P+K
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKL WDARLLTI+Y GI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLHWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VA E  E E   V  EMQ+TS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTS 350

BLAST of HG10008983.1 vs. ExPASy TrEMBL
Match: A0A6J1ESW6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 4.1e-148
Identity = 270/350 (77.14%), Postives = 308/350 (88.00%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M+KLY SCAAMV VQIGYGGSNILMK+A++KDL PFVF VYR+ IAFLVL PFA VIDRN
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           KR+SLTI   IKIFLLAI GS  +LN+FY GI YTS TVASAFSN+ PS TF++A+LFRL
Sbjct: 61  KRASLTISMTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           EKV I+TARG AK+LGT++CIGGSL+FT WKGPY+TKAIFKEPLI+IYKNQDS NSSGK+
Sbjct: 121 EKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSAFI++S+++ S WLILQ F+TRKYPA+LTITVL C+FATAQSGFLAL FAR P+K
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKLQWDARLLTI+Y GI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
           RLHVGSLVGAFFI+LGLYLVLWGK+ADH+VA E  E E      EMQ+TS
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTS 350

BLAST of HG10008983.1 vs. ExPASy TrEMBL
Match: A0A6J1EQM5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.7e-138
Identity = 258/334 (77.25%), Postives = 288/334 (86.23%), Query Frame = 0

Query: 1   MSKLYLSCAAMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRN 60
           M KLY  CAAMV+V I YGGS+IL+KIA+ K L+P VF+VYR+FIAFLVLAPFA +IDRN
Sbjct: 8   MRKLYGPCAAMVLVYIAYGGSHILVKIASNKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67

Query: 61  KRSSLTIPTAIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRL 120
           K +SLTI   I IFLLAI GS IHLN+FYAGI YTSP V S F+NIIPSLTF++A LFRL
Sbjct: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFNNIIPSLTFVMAFLFRL 127

Query: 121 EKVKIRTARGAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKN 180
           E+V IRTARG AK+LGTI CIGGS VFT WKGPYVTK IFKEPLID+YKNQ   + SG++
Sbjct: 128 ERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187

Query: 181 WIKGSAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDK 240
           WIKGSA I++S++A S WLIL   +TRKYPAQLTITVL+CLFATAQSGFLALIFAR  +K
Sbjct: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247

Query: 241 WKLQWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSE 300
           WKL WDARLLTI+Y G+VISG GYFLQTWCIS NGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307

Query: 301 RLHVGSLVGAFFIILGLYLVLWGKQADHMVAAEP 334
           RLHVGSLVGAF IILGLYLVLWGK+ADH VA EP
Sbjct: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of HG10008983.1 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 235.0 bits (598), Expect = 9.9e-62
Identity = 131/324 (40.43%), Postives = 203/324 (62.65%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           AMV VQI Y G  +L K+A  +  +PFVFV YR   A L L+PFA  ++ +K S L+   
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 70  AIKIFLLAISG-SIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            +KIF +++ G ++ LN++Y  I  T+ T A+A +N IPS+TF+LALLFRLE V ++ + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNS---SGKNWIKGSA 189
           G AK+ G+++ + G+LVF   KGP          LI+ Y +    N    S KN +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 190 FILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWD 249
            +L ++    +W+I+Q+ + ++YPA+L +  L CLF+  QS   A+   R+P  WK+++ 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 250 ARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGS 309
             LL++ Y GI+++G+ Y+LQ W I   GPVFT++++PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 310 LVGAFFIILGLYLVLWGKQADHMV 330
           + GA  ++ GLYL LWGK  +  +
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of HG10008983.1 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-60
Identity = 125/356 (35.11%), Postives = 211/356 (59.27%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           AM+ +Q GY G NI+ KI+    +S +V VVYR+ IA  V+APFA   +R  +  +T   
Sbjct: 21  AMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSI 80

Query: 70  AIKIFLLAISGS-IHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            +++F+L + G  I  N +Y G+ YTSPT + A SN++P++TF+LA+LFR+E + ++   
Sbjct: 81  FMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLW 140

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYV----TKAIFKEPLIDIYKNQDSVNSSGKNWIKGS 189
             AK+ GT++ + G+++ T++KGP V    TK +  +            +SS K ++KGS
Sbjct: 141 CQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGS 200

Query: 190 AFILISDIASSVWLILQAFITRKYPA-QLTITVLMCLFATAQSGFLALIFARSPDKWKLQ 249
             ++ + +A +   +LQA I + Y   QL++T L+C   T Q+  +  +   +P  W++ 
Sbjct: 201 ILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIG 260

Query: 250 WDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHV 309
           WD  LL   Y GIV S + Y++Q   +   GPVF + FSPLM++ VA+  +FV +E++ +
Sbjct: 261 WDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFL 320

Query: 310 GSLVGAFFIILGLYLVLWGKQADHMV--AAEPHENENALVSIEMQNTSGKEPLGQG 358
           G ++GA  I++GLY VLWGKQ ++ V        + N+ V+ +++    K  + +G
Sbjct: 321 GGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKISEG 376

BLAST of HG10008983.1 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.4e-60
Identity = 124/340 (36.47%), Postives = 198/340 (58.24%), Query Frame = 0

Query: 11  MVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPTA 70
           M+ +Q G  G+ I++     +  + +V +VYRN +A LVLAPFAL+ +R  R  +T+   
Sbjct: 17  MIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRPKMTLSVL 76

Query: 71  IKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTARG 130
            KI  L  +   +     Y G+  TS T  SA  NI+PS+TF++A + R+EKV I   R 
Sbjct: 77  WKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKVNIAEVRS 136

Query: 131 AAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNSSGKNWIKGSAFILI 190
            AK++GT++ +GG+LV TL+KGP +    +  P +D      + +    NW+ G+  IL+
Sbjct: 137 KAKIIGTLVGLGGALVMTLYKGPLIPLP-WSNPNMDQQNGHTNNSQDHNNWVVGTLLILL 196

Query: 191 SDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWDARLL 250
             +A S + +LQ+   + YPA L+++ L+CL    QS  +AL+  R P  W + WDARL 
Sbjct: 197 GCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVGWDARLF 256

Query: 251 TIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGSLVGA 310
             +Y GIV SG+ Y++Q   +   GPVF + F+PL +I VA+ ++F+  E++H G ++G 
Sbjct: 257 APLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHFGCVIGG 316

Query: 311 FFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTS 350
             I  GLY+V+WGK  D+ V+      +N+L  + +   S
Sbjct: 317 AVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKS 355

BLAST of HG10008983.1 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 228.4 bits (581), Expect = 9.2e-60
Identity = 117/345 (33.91%), Postives = 209/345 (60.58%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           A++ +Q GY G  I+  ++ +  ++ ++   YR+ +A +V+APFAL+++R  R  +T P 
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKMTWPL 73

Query: 70  AIKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
            ++I  L  +   +  N++Y G+  TS T +SAF N +P++TF++A++FR+E V ++  R
Sbjct: 74  FLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTR 133

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIYKNQDSVNS--SGKNWIKGSAF 189
             AK++GT + +GG++V TL+KGP +   +FK     ++      +S  + +NW+ G+  
Sbjct: 134 SLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSGTSSETTDQNWVTGTLA 193

Query: 190 ILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFARSPDKWKLQWDA 249
           ++ S    + + ILQ+F  +KYPA+L++ + +C   T  +   +LI  R    WK+  D+
Sbjct: 194 VMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDS 253

Query: 250 RLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLHVGSL 309
             L  +Y G+V SG+ Y++Q+  I   GPVFT+ FSP+ +I  A     V +E++H+GS+
Sbjct: 254 GTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSI 313

Query: 310 VGAFFIILGLYLVLWGKQADHMVAAEPHENENALVSIEMQNTSGK 352
           +GA FI+ GLY V+WGK  D +++    E +  +  + + NTS K
Sbjct: 314 IGAIFIVFGLYSVVWGKAKDEVISV---EEKIGMQELPITNTSTK 353

BLAST of HG10008983.1 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 226.1 bits (575), Expect = 4.6e-59
Identity = 132/363 (36.36%), Postives = 218/363 (60.06%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIANQKDLSPFVFVVYRNFIAFLVLAPFALVIDRNKRSSLTIPT 69
           +MVV+Q+G  G +IL K    K +S +V VVYR+ +A +V+APFA   D+  R  +T+  
Sbjct: 18  SMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPKMTLMI 77

Query: 70  AIKIFLLA-ISGSIHLNMFYAGIGYTSPTVASAFSNIIPSLTFLLALLFRLEKVKIRTAR 129
             KI LL  +   I  N++Y G+ YT+ T A+A  N++P++TF+LA +F LE+VK+R  R
Sbjct: 78  FFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVKLRCIR 137

Query: 130 GAAKLLGTILCIGGSLVFTLWKGPYVTKAIFKEPLIDIY--KNQDSVNSSG---KNWIKG 189
              K++GT+  +GG+++ TL KG          P++D++  K   + N++G    + IKG
Sbjct: 138 STGKVVGTLATVGGAMIMTLVKG----------PVLDLFWTKGVSAHNTAGTDIHSAIKG 197

Query: 190 SAFILISDIASSVWLILQAFITRKYPAQLTITVLMCLFATAQSGFLALIFAR-SPDKWKL 249
           +  + I   + + ++ILQA   R YPA+L++T  +CL  T +   +AL+  + +P  W +
Sbjct: 198 AVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAI 257

Query: 250 QWDARLLTIIYGGIVISGVGYFLQTWCISCNGPVFTSMFSPLMLIFVAIFSAFVFSERLH 309
            WD +LLT  Y GIV S + Y++    +   GPVF + FSPL +I VAI S  +F+E+++
Sbjct: 258 GWDTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMY 317

Query: 310 VGSLVGAFFIILGLYLVLWGKQADHMVAAEPH-ENENALVSIEMQNTSGKEPLGQGVELF 365
           +G ++GA  I  GLYLV+WGK  D+   +    ++E+A   +E+   +GK+ +   V   
Sbjct: 318 LGRVLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSG-NGKDNVDHEVITI 369