Homology
BLAST of HG10005272.1 vs. NCBI nr
Match:
XP_038888073.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Benincasa hispida])
HSP 1 Score: 1455.7 bits (3767), Expect = 0.0e+00
Identity = 715/767 (93.22%), Postives = 736/767 (95.96%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFP LDVDTKTKTGALR EETKERI++LFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 72 SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 131
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLDNQHPDGSWGILHDHQ L KATLLSTLAC+LTLKRWNIGQDHMSKALSFIK
Sbjct: 132 FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 191
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSEG 201
SNIASATDEKQRSPVGFDIIFPGMIEY KDLNLNL LAPM VDALVQKKELELRRSNSEG
Sbjct: 192 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRRSNSEG 251
Query: 202 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQK 261
GKAYLAYVSEGI LQDWEMVMQYQRKNGSLFNSP+TTAVAFMHRNDDGCFNYLRSVLQK
Sbjct: 252 GKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVLQK 311
Query: 262 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDAST 321
FD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEI+SVLDETYRCWMQGEENIFLDAST
Sbjct: 312 FDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDAST 371
Query: 322 CAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDESIL 381
CAMAFR+L VEGYDVSSD+LTQFSE LF NCLGGHLKDFGAS+ELFKASQ++I PDESIL
Sbjct: 372 CAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDESIL 431
Query: 382 ENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYSGD 441
ENVNSWTSRFLNHGLS G V SDRTD LVKQEAVNALEFPYNATLERL NKRALESYSGD
Sbjct: 432 ENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYSGD 491
Query: 442 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL 501
IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL
Sbjct: 492 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL 551
Query: 502 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANG 561
AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEEL+NLIQLVEKWDANG
Sbjct: 552 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDANG 611
Query: 562 ETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTK 621
ETGYCSKEVEIIFL LHSTVC+IG RAL+WQGRSV RNVIDIWLALLESMRKEAEWLK K
Sbjct: 612 ETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 671
Query: 622 VVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT 681
VVPSMDEYMENGYVSFALGPIVLP LYF+GPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT
Sbjct: 672 VVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT 731
Query: 682 RTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIPRA 741
RT DRESSEGKLNA+SLC+I+AGGK KEEATEAMKGDVERTRRELLRLVLQEN+TIPRA
Sbjct: 732 RTFDRESSEGKLNAMSLCIINAGGKLTKEEATEAMKGDVERTRRELLRLVLQENTTIPRA 791
Query: 742 CKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
CKDLF KMSCVVHLFYRKDDGFTSHELM SAKALFEQPMVLD+LLNK
Sbjct: 792 CKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVLDQLLNK 838
BLAST of HG10005272.1 vs. NCBI nr
Match:
XP_038888074.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida])
HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 715/769 (92.98%), Postives = 736/769 (95.71%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFP LDVDTKTKTGALR EETKERI++LFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17 SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 76
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLDNQHPDGSWGILHDHQ L KATLLSTLAC+LTLKRWNIGQDHMSKALSFIK
Sbjct: 77 FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 136
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELR--RSNS 201
SNIASATDEKQRSPVGFDIIFPGMIEY KDLNLNL LAPM VDALVQKKELELR RSNS
Sbjct: 137 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRSCRSNS 196
Query: 202 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
EGGKAYLAYVSEGI LQDWEMVMQYQRKNGSLFNSP+TTAVAFMHRNDDGCFNYLRSVL
Sbjct: 197 EGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVL 256
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEI+SVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDA 316
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
STCAMAFR+L VEGYDVSSD+LTQFSE LF NCLGGHLKDFGAS+ELFKASQ++I PDES
Sbjct: 317 STCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDES 376
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
ILENVNSWTSRFLNHGLS G V SDRTD LVKQEAVNALEFPYNATLERL NKRALESYS
Sbjct: 377 ILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYS 436
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 496
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEEL+NLIQLVEKWDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDA 556
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
NGETGYCSKEVEIIFL LHSTVC+IG RAL+WQGRSV RNVIDIWLALLESMRKEAEWLK
Sbjct: 557 NGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLK 616
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
KVVPSMDEYMENGYVSFALGPIVLP LYF+GPKLPEEVIGNCEYKKLFKLMSTCGRLLN
Sbjct: 617 NKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 676
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIP 741
DTRT DRESSEGKLNA+SLC+I+AGGK KEEATEAMKGDVERTRRELLRLVLQEN+TIP
Sbjct: 677 DTRTFDRESSEGKLNAMSLCIINAGGKLTKEEATEAMKGDVERTRRELLRLVLQENTTIP 736
Query: 742 RACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
RACKDLF KMSCVVHLFYRKDDGFTSHELM SAKALFEQPMVLD+LLNK
Sbjct: 737 RACKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVLDQLLNK 785
BLAST of HG10005272.1 vs. NCBI nr
Match:
XP_038888071.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_038888072.1 ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 715/769 (92.98%), Postives = 736/769 (95.71%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFP LDVDTKTKTGALR EETKERI++LFDKVELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 72 SASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPL 131
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLDNQHPDGSWGILHDHQ L KATLLSTLAC+LTLKRWNIGQDHMSKALSFIK
Sbjct: 132 FPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSKALSFIK 191
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELR--RSNS 201
SNIASATDEKQRSPVGFDIIFPGMIEY KDLNLNL LAPM VDALVQKKELELR RSNS
Sbjct: 192 SNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELRSCRSNS 251
Query: 202 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
EGGKAYLAYVSEGI LQDWEMVMQYQRKNGSLFNSP+TTAVAFMHRNDDGCFNYLRSVL
Sbjct: 252 EGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVL 311
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEI+SVLDETYRCWMQGEENIFLDA
Sbjct: 312 QKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDA 371
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
STCAMAFR+L VEGYDVSSD+LTQFSE LF NCLGGHLKDFGAS+ELFKASQ++I PDES
Sbjct: 372 STCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQIIIYPDES 431
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
ILENVNSWTSRFLNHGLS G V SDRTD LVKQEAVNALEFPYNATLERL NKRALESYS
Sbjct: 432 ILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKRALESYS 491
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ
Sbjct: 492 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 551
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEEL+NLIQLVEKWDA
Sbjct: 552 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDA 611
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
NGETGYCSKEVEIIFL LHSTVC+IG RAL+WQGRSV RNVIDIWLALLESMRKEAEWLK
Sbjct: 612 NGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRKEAEWLK 671
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
KVVPSMDEYMENGYVSFALGPIVLP LYF+GPKLPEEVIGNCEYKKLFKLMSTCGRLLN
Sbjct: 672 NKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 731
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIP 741
DTRT DRESSEGKLNA+SLC+I+AGGK KEEATEAMKGDVERTRRELLRLVLQEN+TIP
Sbjct: 732 DTRTFDRESSEGKLNAMSLCIINAGGKLTKEEATEAMKGDVERTRRELLRLVLQENTTIP 791
Query: 742 RACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
RACKDLF KMSCVVHLFYRKDDGFTSHELM SAKALFEQPMVLD+LLNK
Sbjct: 792 RACKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVLDQLLNK 840
BLAST of HG10005272.1 vs. NCBI nr
Match:
XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])
HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 703/767 (91.66%), Postives = 736/767 (95.96%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17 SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSKALSFIK
Sbjct: 77 FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSEG 201
SNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDALVQKKELEL RSNSEG
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEG 196
Query: 202 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQK 261
GKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SP+TTAVAFMHRNDDGCFNYLRS+LQK
Sbjct: 197 GKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQK 256
Query: 262 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDAST 321
F SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EI+SVLDETYRCWMQGEENIFLDAST
Sbjct: 257 FHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAST 316
Query: 322 CAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDESIL 381
CAMAFRML VEGYDVSSDQLTQFSEDLF NCLGGHLKDFGASLELFKASQ++I PDE IL
Sbjct: 317 CAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFIL 376
Query: 382 ENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYSGD 441
EN+NSWTSRFLNHGLS GSVHSDRT+ LVKQEAVNA EFPYN+TLERLSNKRALESYSGD
Sbjct: 377 ENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGD 436
Query: 442 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL 501
IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWV+ENKLDKLKFARQKL
Sbjct: 437 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKL 496
Query: 502 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANG 561
AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA+G
Sbjct: 497 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASG 556
Query: 562 ETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTK 621
E GYCSKEVEIIFL LHST+CEIG +AL WQGRSV RNVIDIWLALLESMRKEAEWLK K
Sbjct: 557 EAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 616
Query: 622 VVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT 681
VVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE+IGNCEY+KLFKLMST GRLLNDT
Sbjct: 617 VVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDT 676
Query: 682 RTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIPRA 741
RT DRESSEGKLNA+SL +ISAGGK +KEEATEAMKGDV+RTRRELLRLVLQENSTIPRA
Sbjct: 677 RTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQENSTIPRA 736
Query: 742 CKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
CKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFE PMVLDELLNK
Sbjct: 737 CKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 782
BLAST of HG10005272.1 vs. NCBI nr
Match:
XP_008447073.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo])
HSP 1 Score: 1437.2 bits (3719), Expect = 0.0e+00
Identity = 703/769 (91.42%), Postives = 736/769 (95.71%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17 SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSKALSFIK
Sbjct: 77 FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRR--SNS 201
SNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDALVQKKELELR SNS
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNS 196
Query: 202 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SP+TTAVAFMHRNDDGCFNYLRS+L
Sbjct: 197 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSIL 256
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
QKF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EI+SVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDA 316
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
STCAMAFRML VEGYDVSSDQLTQFSEDLF NCLGGHLKDFGASLELFKASQ++I PDE
Sbjct: 317 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEF 376
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
ILEN+NSWTSRFLNHGLS GSVHSDRT+ LVKQEAVNA EFPYN+TLERLSNKRALESYS
Sbjct: 377 ILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYS 436
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWV+ENKLDKLKFARQ
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQ 496
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 556
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
+GE GYCSKEVEIIFL LHST+CEIG +AL WQGRSV RNVIDIWLALLESMRKEAEWLK
Sbjct: 557 SGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLK 616
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
KVVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE+IGNCEY+KLFKLMST GRLLN
Sbjct: 617 NKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLN 676
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIP 741
DTRT DRESSEGKLNA+SL +ISAGGK +KEEATEAMKGDV+RTRRELLRLVLQENSTIP
Sbjct: 677 DTRTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQENSTIP 736
Query: 742 RACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
RACKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFE PMVLDELLNK
Sbjct: 737 RACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 785
BLAST of HG10005272.1 vs. ExPASy Swiss-Prot
Match:
Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)
HSP 1 Score: 1288.1 bits (3332), Expect = 0.0e+00
Identity = 628/769 (81.66%), Postives = 691/769 (89.86%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFPG+DVDT TKTGAL FEETKERI++LFDKVELSVSAYDTAWVAMVPSPNSLN+PL
Sbjct: 21 SASLFPGVDVDTTTKTGALHFEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNQPL 80
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLD+QH DGSWG+LH+ Q L KA LLSTLAC+LTLKRWNIG DHMSKAL FIK
Sbjct: 81 FPECINWVLDSQHADGSWGLLHNDQLLMKANLLSTLACVLTLKRWNIGHDHMSKALDFIK 140
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELR--RSNS 201
SNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LAP NVDALV+KKELELR RSNS
Sbjct: 141 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPTNVDALVRKKELELRSCRSNS 200
Query: 202 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLFNSP+TTA AFMHRNDDGCF+YLRS+L
Sbjct: 201 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPSTTAAAFMHRNDDGCFDYLRSLL 260
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
QKFD SVPTIYPLDIYARLHMVDSLQK GI RHFKEEI+SVLDETYRCWMQGEENIFLDA
Sbjct: 261 QKFDGSVPTIYPLDIYARLHMVDSLQKFGIARHFKEEIRSVLDETYRCWMQGEENIFLDA 320
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
STCAMAFRML VEGYDVSSDQLTQFSED+F NCLGG+LKDFGASLEL+KASQ++ PDES
Sbjct: 321 STCAMAFRMLRVEGYDVSSDQLTQFSEDIFPNCLGGYLKDFGASLELYKASQIITHPDES 380
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
+LEN+NSWTSRFL HGLSS SV SDRTD++VKQEAVNALEFPYNATLERL +KRA+ESYS
Sbjct: 381 VLENINSWTSRFLKHGLSSDSVWSDRTDSVVKQEAVNALEFPYNATLERLISKRAMESYS 440
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
GDIVRISK+ YACLNFGHQDFLELAVEDFNTLQ IH KEL+EL++WVVENKLD+LKF R
Sbjct: 441 GDIVRISKSPYACLNFGHQDFLELAVEDFNTLQRIHLKELEELQRWVVENKLDELKFFRL 500
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
L YCYF+AAATLT PEL DAR++WA+NGVLTTVVDDF+D GGSEEEL NLI+LVEKWD
Sbjct: 501 HLGYCYFAAAATLTDPELHDARIAWAQNGVLTTVVDDFYDGGGSEEELDNLIELVEKWDP 560
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
+GE GYCSK+VEI+FL LHSTVCEIG RAL WQGRSVMRNVID WLALL+ MRKEAEW
Sbjct: 561 DGEVGYCSKDVEIVFLALHSTVCEIGRRALVWQGRSVMRNVIDGWLALLKVMRKEAEWST 620
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
KVVPSM EYME +VSFALGPI+LP L+F+GPKL EE+IG+CEY+KL+KLMST GRL N
Sbjct: 621 NKVVPSMGEYMEQAHVSFALGPIILPMLFFVGPKLSEEMIGSCEYQKLYKLMSTAGRLKN 680
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIP 741
D R+ DRE EGKLN +SL MI GG KEEA EA+KGD ER RELL LVLQEN+TIP
Sbjct: 681 DIRSYDRECKEGKLNILSLWMIDGGGNVTKEEAIEAIKGDFERAIRELLGLVLQENTTIP 740
Query: 742 RACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
RACKDLFWK+ +V+LFY +DDG+TS+ LM++ KA+FEQPM LD LLNK
Sbjct: 741 RACKDLFWKLMSIVNLFYMEDDGYTSNRLMNTVKAMFEQPMDLDALLNK 789
BLAST of HG10005272.1 vs. ExPASy Swiss-Prot
Match:
A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)
HSP 1 Score: 870.5 bits (2248), Expect = 1.4e-251
Identity = 426/764 (55.76%), Postives = 560/764 (73.30%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASL G+ T K + FEETKERI +L K ELSVS YDTAWVAMVPSPNS +P
Sbjct: 25 SASLDTGVRRVTSAKIASTCFEETKERIADLIHKAELSVSTYDTAWVAMVPSPNSSQEPC 84
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FP+C+ W+L NQ DGSW H H LKK L STLAC+L LK+W +G++ +++ + FI+
Sbjct: 85 FPDCLSWLLQNQCCDGSWACPHHHPLLKKDVLCSTLACVLALKKWGVGEEKINRGVHFIE 144
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSEG 201
N ASA ++ Q SP+GFDIIFP M++Y +DL LNL L P ++ L+ K+ LEL+R+ +
Sbjct: 145 HNFASAMEKCQISPMGFDIIFPAMLDYARDLLLNLRLEPTMLNDLIYKRGLELKRNQNHS 204
Query: 202 G--KAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
+AYLAYV+EG+GKLQD VM++QR+NGSLFNSP+TTA AF+ + C YLRS L
Sbjct: 205 AEREAYLAYVAEGMGKLQDLGSVMKHQRRNGSLFNSPSTTAAAFIAFPNSRCLTYLRSAL 264
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
+KF S+VP +YPLDIY +L VD+L++LGI R+F++EI+ VLDETYRCW+QG E IF+DA
Sbjct: 265 KKFGSAVPAVYPLDIYLQLCTVDNLERLGISRYFQKEIQGVLDETYRCWLQGNEEIFMDA 324
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
TCA+AFR+L GY+V+SD +T+ E+ FS+ G++KD +L L++AS+ ++ PDE
Sbjct: 325 PTCALAFRVLRKNGYNVTSDPITKLLEECFSSSFCGNIKDINTTLGLYRASEFILYPDER 384
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
LE N L LSS +HS + + E +ALE+P+ A L+R+ N+R +E Y+
Sbjct: 385 DLEKQNLMLKNLLEQELSSDFIHSSQLGRNIDAEVKHALEYPFYADLDRIVNRRNIEHYN 444
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
D RI KT+Y NFG++DFL L+V+D+N Q IHR+EL+ELE+WV+EN+LD+L+FARQ
Sbjct: 445 FDNTRILKTSYCSPNFGNKDFLFLSVKDYNECQAIHREELRELERWVIENRLDELRFARQ 504
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
K AYCYFSAAATL +PEL +AR+SWAKNGVLTTVVDDFFD+GGS EEL NLIQLVE WD
Sbjct: 505 KCAYCYFSAAATLFAPELSNARMSWAKNGVLTTVVDDFFDLGGSVEELKNLIQLVELWDV 564
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
+ T S+ V+IIF L T+C+IG++ Q RS+ ++IDIWL LL SM KE EW +
Sbjct: 565 DVSTECSSQNVQIIFSALKCTICDIGDKGSKLQERSITNHIIDIWLDLLYSMMKETEWAR 624
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
K +P+MDEY+ N YVSFALGPIVLPALY +GPKL EE++ + EY L+KLMSTCGRLLN
Sbjct: 625 DKYIPTMDEYISNAYVSFALGPIVLPALYLVGPKLSEEMVHHSEYHNLYKLMSTCGRLLN 684
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQ-ENSTI 741
D R +RE EGKLNA+ L +I+ GG+ KE A MK +E RRELLRLVL+ +NS +
Sbjct: 685 DIRGCERELKEGKLNAIPLYIINNGGEITKEAAASEMKSLIETHRRELLRLVLEGKNSVL 744
Query: 742 PRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVL 783
P++CK+LFW MS V+HLFY KDDGFTS +L+ KA+ +P+VL
Sbjct: 745 PKSCKELFWHMSKVLHLFYSKDDGFTSQDLIKVVKAVIYEPIVL 788
BLAST of HG10005272.1 vs. ExPASy Swiss-Prot
Match:
A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)
HSP 1 Score: 857.1 bits (2213), Expect = 1.6e-247
Identity = 426/770 (55.32%), Postives = 559/770 (72.60%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALR--FEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNK 81
+ASL GL T + ++ FEETK RI +LF K ELSVS YDTAWVAMVPSP S +
Sbjct: 24 SASLDIGLQRATSPQNASVATCFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEE 83
Query: 82 PLFPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSF 141
P FP C++W+L+NQ DGSW H H LKK L STLACIL LK+W +G++ +++ L F
Sbjct: 84 PCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHF 143
Query: 142 IKSNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRR--- 201
++ N ASAT++ Q +P+GFDIIFP M++ + L+LNL L P ++ L+ K++LEL+R
Sbjct: 144 VELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPTTLNDLIYKRDLELKRCNQ 203
Query: 202 SNSEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLR 261
SNS + Y A+++EG+GKLQDWE VM+YQRKNGSLFN P+TTA AF + C NYLR
Sbjct: 204 SNSAEKEVYWAHIAEGMGKLQDWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLR 263
Query: 262 SVLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIF 321
L+KF S+VP +YPLDIY++L VD+L++LGI R+F EI+SVLDETYR W+QG+E IF
Sbjct: 264 LALEKFGSAVPAVYPLDIYSQLCTVDNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIF 323
Query: 322 LDASTCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRP 381
+DASTCA+AFR L + GY+V+SD +T+ ++ FS+ G++ D +LE+++AS+L++ P
Sbjct: 324 MDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMTDINTTLEIYRASELILYP 383
Query: 382 DESILENVNSWTSRFLNHGLSS-GSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRAL 441
+E L+ N FL LSS G + S + + E A+E+P+ A ++R++ ++ +
Sbjct: 384 EERDLDQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNI 443
Query: 442 ESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLK 501
E+Y+ D RI KT+Y NFG++DFL L+VEDFN Q IHR+EL+ELE+WV+EN+LD+LK
Sbjct: 444 ENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELK 503
Query: 502 FARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVE 561
FAR K AYCYFSAAAT+ SPEL DAR+SWAKNGVLTTVVDDFFDVGGS EEL NLIQLVE
Sbjct: 504 FARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVE 563
Query: 562 KWDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEA 621
WD + T CS V+IIF L T+CEI ++ QGRS+ ++I IWL LL SM KE
Sbjct: 564 LWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKET 623
Query: 622 EWLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCG 681
E K P+MDEYM N YVSFALGPIVLPALY +GPKL EE++ + EY LFKLMSTCG
Sbjct: 624 ELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCG 683
Query: 682 RLLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQEN 741
RLLND R +RE +GK++AVSL +++ GG+ E A M+ +ER RRELLRLVL+EN
Sbjct: 684 RLLNDIRGYERELKDGKISAVSLYIMNNGGEITTEAAISEMRSWIERDRRELLRLVLEEN 743
Query: 742 -STIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDE 785
S +P+ACK LFW M VVHLFYRKDDGFTS +L A+ +P+VL++
Sbjct: 744 KSVLPKACKKLFWHMCTVVHLFYRKDDGFTSLDLHGVVNAIINEPIVLNQ 793
BLAST of HG10005272.1 vs. ExPASy Swiss-Prot
Match:
A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)
HSP 1 Score: 848.2 bits (2190), Expect = 7.5e-245
Identity = 415/769 (53.97%), Postives = 552/769 (71.78%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALR--FEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNK 81
+ASL GL T K ++ FE+T+ RI +LF K ELSVS YDTAWVAMVPSP SL +
Sbjct: 27 SASLKSGLHSATSAKIASMPTCFEQTRGRIAKLFHKDELSVSTYDTAWVAMVPSPTSLEE 86
Query: 82 PLFPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSF 141
P FP+C++W+L+NQ DGSW H H LKK L STLACIL LK+W +G++ + + L F
Sbjct: 87 PCFPDCLNWLLENQCHDGSWARPHHHPLLKKDVLSSTLACILALKKWGVGEEQIKRGLHF 146
Query: 142 IKSNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSN- 201
++ N ASATD+ Q +P+GFDIIFP M++Y + +LNL L P + L+ K+++EL+RSN
Sbjct: 147 LELNFASATDKCQITPMGFDIIFPAMLDYARGFSLNLRLDPTTFNDLMHKRDVELKRSNR 206
Query: 202 --SEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLR 261
S + Y AY++EG+G+LQ+WE VM+YQR+NGSLFN P+TTA AF+ + C NYL
Sbjct: 207 NYSSETETYWAYIAEGMGELQNWESVMKYQRRNGSLFNCPSTTAAAFIALRNSDCLNYLH 266
Query: 262 SVLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIF 321
L+KF ++V +YPLDIY++L VD+L++LGI ++F EI++VLDETYRCWMQG E IF
Sbjct: 267 LALKKFGNAVSAVYPLDIYSQLCTVDNLERLGISQYFSTEIQNVLDETYRCWMQGNEEIF 326
Query: 322 LDASTCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRP 381
+DASTCA+AFR L + GYDV+SD +TQ ++ FS+ G++ D +LEL++AS+L++ P
Sbjct: 327 MDASTCALAFRTLRLNGYDVTSDPVTQILQECFSSSFRGNMTDINTTLELYRASELVLYP 386
Query: 382 DESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALE 441
DE LE N L LSSG + S + + E A+E+P+ A ++R++ ++++E
Sbjct: 387 DERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVEVNQAIEYPFYAIMDRVAKRKSIE 446
Query: 442 SYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKF 501
Y+ D RI KT+Y NF ++DFL L++EDFN Q HR+EL ELE+WVVEN+LD+LKF
Sbjct: 447 HYNFDNTRILKTSYCSPNFSNEDFLFLSIEDFNRCQAAHREELGELERWVVENRLDELKF 506
Query: 502 ARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEK 561
AR K AYCYFSAAAT +PEL DARLSWAKNGVLTTV+DDFFDVGGS EEL NLIQLVE
Sbjct: 507 ARSKSAYCYFSAAATFFAPELLDARLSWAKNGVLTTVIDDFFDVGGSVEELKNLIQLVEL 566
Query: 562 WDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAE 621
WD + T S V+IIF L T+CEIG++A QGR + ++I IWL LL SM +E E
Sbjct: 567 WDVDISTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHIIAIWLDLLNSMMRETE 626
Query: 622 WLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGR 681
W + VP++DEYM N +VSFALGPIVLPALY +GPKL EE++ + EY LFKLMSTCGR
Sbjct: 627 WARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEEMVNHSEYHNLFKLMSTCGR 686
Query: 682 LLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQ-EN 741
LLND +RE +GKLNA+SL +I+ GG+ +KE A MK +E RRELLRLVL+ +
Sbjct: 687 LLNDIHGYERELKDGKLNALSLYIINHGGEVSKEAAIWEMKSWIETQRRELLRLVLEGKK 746
Query: 742 STIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDE 785
S +P+ C++LFW M VVHLFY K DGFTS +L+ + QP++L++
Sbjct: 747 SVLPKPCRELFWHMCSVVHLFYSKGDGFTSQDLIQLVNTIIHQPILLND 795
BLAST of HG10005272.1 vs. ExPASy Swiss-Prot
Match:
A0A075FBT3 (Ent-kaurene synthase, chloroplastic (Fragment) OS=Marrubium vulgare OX=41230 GN=EKS PE=1 SV=1)
HSP 1 Score: 847.0 bits (2187), Expect = 1.7e-244
Identity = 417/743 (56.12%), Postives = 541/743 (72.81%), Query Frame = 0
Query: 42 FEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPLFPECIDWVLDNQHPDGSWGI 101
FE+TK RI +L K ELSVS YDTAWVAMVPSPNS N+P FP+C+ W+L+NQ DGSW
Sbjct: 27 FEDTKARIVKLIHKPELSVSTYDTAWVAMVPSPNSSNEPCFPDCLAWLLENQCCDGSWAC 86
Query: 102 LHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIKSNIASATDEKQRSPVGFDII 161
H H FLKK L STLACIL LK+W +G++ ++K FI+ N ASAT++ Q SP GFDII
Sbjct: 87 PHHHPFLKKDVLSSTLACILALKKWGVGEEQINKGARFIEVNFASATEKSQISPTGFDII 146
Query: 162 FPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSEGGKAYLAYVSEGIGKLQDWEM 221
FP M++Y +DL L+L L P D L+ +++LEL+R +AYLAY++EG+GKLQDWE
Sbjct: 147 FPAMLDYARDLFLDLRLEPTTFDNLIFRRDLELKRCYESHREAYLAYIAEGMGKLQDWES 206
Query: 222 VMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQKFDSSVPTIYPLDIYARLHMV 281
VM+YQRKNGSLFNSP+TTA AF+ + GC +YL S L+KF ++VP YPLD+Y+RL V
Sbjct: 207 VMKYQRKNGSLFNSPSTTAAAFIALPNSGCLSYLHSALKKFGNAVPAAYPLDVYSRLRTV 266
Query: 282 DSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDASTCAMAFRMLCVEGYDVSSDQL 341
D+L+ LGI R+F++EI+ VLDETYR W+QG E IFLDASTCA+AFR L + GY+V+SD +
Sbjct: 267 DNLESLGISRYFQKEIQQVLDETYRWWLQGSEEIFLDASTCALAFRTLRMNGYNVTSDAI 326
Query: 342 TQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDESILENVNSWTSRFLNHGLSSGSV 401
T+ D F G++KD G +LEL++AS+ ++ PDE LE N L LSSG +
Sbjct: 327 TKLLPDSFC----GNMKDIGTTLELYRASEFILYPDEKDLEQQNLRLKDILEQELSSGFI 386
Query: 402 HSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFL 461
HS E AL +P+ A ++R++ +R +E Y+ D RI KT+Y NF ++DFL
Sbjct: 387 HS---------EVNRALNYPFYAIMDRVAKRRNIEHYNFDNTRILKTSYCSPNFANKDFL 446
Query: 462 ELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKLAYCYFSAAATLTSPELCDAR 521
L+VEDFN Q +HR+ELK +WV EN+LD+LKFAR K AYCYFSAAAT +P+L DAR
Sbjct: 447 FLSVEDFNNCQAMHREELKGKMRWVTENRLDELKFARSKSAYCYFSAAATFFAPDLSDAR 506
Query: 522 LSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANGETGYCSKEVEIIFLTLHSTV 581
+SWAKNGVLTTVVDDFFDVGGSEEEL LI+LVE WD + T S+ V+IIF +L T+
Sbjct: 507 MSWAKNGVLTTVVDDFFDVGGSEEELKQLIRLVEIWDLDAITECSSQNVQIIFSSLKRTI 566
Query: 582 CEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTKVVPSMDEYMENGYVSFALGP 641
EIG++ QGRSV ++I+IWL LL SM KEAEW + P MD+Y+ N YVSFALGP
Sbjct: 567 SEIGDKGFKLQGRSVTNHIIEIWLDLLYSMMKEAEWARDNHAPPMDDYISNAYVSFALGP 626
Query: 642 IVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDTRTVDRESSEGKLNAVSLCMI 701
IVLP LY +GPKL EE++ + E LF+LMSTCGRLLND +T +RE +GKLNA+ L MI
Sbjct: 627 IVLPCLYLVGPKLSEEMVRHSECHTLFRLMSTCGRLLNDIQTCERELKDGKLNAIPLYMI 686
Query: 702 SAGGKFNKEEATEAMKGDVERTRRELLRLVL-QENSTIPRACKDLFWKMSCVVHLFYRKD 761
++GG+ +KE AT+ MK ++R R+ELLRLVL +E S +P+ CK+LFW MS V+HLFY KD
Sbjct: 687 NSGGETSKEAATQEMKSLIDRQRQELLRLVLTREGSLLPKPCKELFWHMSTVLHLFYCKD 746
Query: 762 DGFTSHELMSSAKALFEQPMVLD 784
DGFTS +L+ + +P+VL+
Sbjct: 747 DGFTSQDLIKVVNEVIHEPVVLN 756
BLAST of HG10005272.1 vs. ExPASy TrEMBL
Match:
A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 703/767 (91.66%), Postives = 736/767 (95.96%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17 SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSKALSFIK
Sbjct: 77 FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSEG 201
SNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDALVQKKELEL RSNSEG
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEG 196
Query: 202 GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQK 261
GKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SP+TTAVAFMHRNDDGCFNYLRS+LQK
Sbjct: 197 GKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQK 256
Query: 262 FDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDAST 321
F SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EI+SVLDETYRCWMQGEENIFLDAST
Sbjct: 257 FHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAST 316
Query: 322 CAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDESIL 381
CAMAFRML VEGYDVSSDQLTQFSEDLF NCLGGHLKDFGASLELFKASQ++I PDE IL
Sbjct: 317 CAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFIL 376
Query: 382 ENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYSGD 441
EN+NSWTSRFLNHGLS GSVHSDRT+ LVKQEAVNA EFPYN+TLERLSNKRALESYSGD
Sbjct: 377 ENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGD 436
Query: 442 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKL 501
IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWV+ENKLDKLKFARQKL
Sbjct: 437 IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKL 496
Query: 502 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANG 561
AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA+G
Sbjct: 497 AYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASG 556
Query: 562 ETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTK 621
E GYCSKEVEIIFL LHST+CEIG +AL WQGRSV RNVIDIWLALLESMRKEAEWLK K
Sbjct: 557 EAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNK 616
Query: 622 VVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDT 681
VVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE+IGNCEY+KLFKLMST GRLLNDT
Sbjct: 617 VVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDT 676
Query: 682 RTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIPRA 741
RT DRESSEGKLNA+SL +ISAGGK +KEEATEAMKGDV+RTRRELLRLVLQENSTIPRA
Sbjct: 677 RTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQENSTIPRA 736
Query: 742 CKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
CKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFE PMVLDELLNK
Sbjct: 737 CKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 782
BLAST of HG10005272.1 vs. ExPASy TrEMBL
Match:
A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1437.2 bits (3719), Expect = 0.0e+00
Identity = 703/769 (91.42%), Postives = 736/769 (95.71%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+ASLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLNKPL
Sbjct: 17 SASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPL 76
Query: 82 FPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIK 141
FPECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSKALSFIK
Sbjct: 77 FPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIK 136
Query: 142 SNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRR--SNS 201
SNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDALVQKKELELR SNS
Sbjct: 137 SNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNS 196
Query: 202 EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVL 261
EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SP+TTAVAFMHRNDDGCFNYLRS+L
Sbjct: 197 EGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSIL 256
Query: 262 QKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDA 321
QKF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EI+SVLDETYRCWMQGEENIFLDA
Sbjct: 257 QKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDA 316
Query: 322 STCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDES 381
STCAMAFRML VEGYDVSSDQLTQFSEDLF NCLGGHLKDFGASLELFKASQ++I PDE
Sbjct: 317 STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEF 376
Query: 382 ILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYS 441
ILEN+NSWTSRFLNHGLS GSVHSDRT+ LVKQEAVNA EFPYN+TLERLSNKRALESYS
Sbjct: 377 ILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYS 436
Query: 442 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQ 501
GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWV+ENKLDKLKFARQ
Sbjct: 437 GDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQ 496
Query: 502 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 561
KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA
Sbjct: 497 KLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA 556
Query: 562 NGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLK 621
+GE GYCSKEVEIIFL LHST+CEIG +AL WQGRSV RNVIDIWLALLESMRKEAEWLK
Sbjct: 557 SGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLK 616
Query: 622 TKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLN 681
KVVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE+IGNCEY+KLFKLMST GRLLN
Sbjct: 617 NKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLN 676
Query: 682 DTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIP 741
DTRT DRESSEGKLNA+SL +ISAGGK +KEEATEAMKGDV+RTRRELLRLVLQENSTIP
Sbjct: 677 DTRTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQENSTIP 736
Query: 742 RACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
RACKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFE PMVLDELLNK
Sbjct: 737 RACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 785
BLAST of HG10005272.1 vs. ExPASy TrEMBL
Match:
A0A5A7V5B3 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold357G00260 PE=4 SV=1)
HSP 1 Score: 1432.2 bits (3706), Expect = 0.0e+00
Identity = 700/768 (91.15%), Postives = 734/768 (95.57%), Query Frame = 0
Query: 23 ASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPLF 82
+SLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLN+PLF
Sbjct: 92 SSLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNRPLF 151
Query: 83 PECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIKS 142
PECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSKALSFIKS
Sbjct: 152 PECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKS 211
Query: 143 NIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRR--SNSE 202
NIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDALVQKKELELR SNSE
Sbjct: 212 NIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNSE 271
Query: 203 GGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQ 262
GGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SP+TTAVAFMHRNDDGCFNYLRS+LQ
Sbjct: 272 GGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQ 331
Query: 263 KFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFLDAS 322
KF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+EI+SVLDETYRCWMQGEENIFLDAS
Sbjct: 332 KFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDAS 391
Query: 323 TCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASLELFKASQLLIRPDESI 382
TCAMAFRML VEGYDVSSDQLTQFSEDLF NCLGGHLKDFGASLELFKASQ++I PDE I
Sbjct: 392 TCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFI 451
Query: 383 LENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALESYSG 442
LEN+NSWTSRFLNHGLS GSVH DRT+ LVKQEAVNA EFPYN+TLERLSNKRALESYSG
Sbjct: 452 LENINSWTSRFLNHGLSGGSVHFDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSG 511
Query: 443 DIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLKFARQK 502
DIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWV+ENKLDKLKFARQK
Sbjct: 512 DIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQK 571
Query: 503 LAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDAN 562
LAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDA+
Sbjct: 572 LAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDAS 631
Query: 563 GETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKT 622
GE GYCSKEVEIIFL LHST+CEIG +AL WQGRSV RNVIDIWLALLESMRKEAEWLK
Sbjct: 632 GEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKN 691
Query: 623 KVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLND 682
KVVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE+IGNCEY+KLFKLMST GRLLND
Sbjct: 692 KVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLND 751
Query: 683 TRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQENSTIPR 742
TRT DRESSEGKLNA+SL +ISAGGK +KEEATEAMKGDV+RTRRELLRLVLQENSTIPR
Sbjct: 752 TRTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQENSTIPR 811
Query: 743 ACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDELLNK 789
ACKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFE PMVLDELLNK
Sbjct: 812 ACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 859
BLAST of HG10005272.1 vs. ExPASy TrEMBL
Match:
Q9FRX5 (Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1)
HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 705/785 (89.81%), Postives = 737/785 (93.89%), Query Frame = 0
Query: 6 VRLSNKAVLSMDEYMENASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDT 65
+ LS L +ASLFPGLDV TKTKTGALRFEETKERI++LF VELS+SAYDT
Sbjct: 1 MNLSRPTNLGCFTASSSASLFPGLDVGTKTKTGALRFEETKERIKKLFKNVELSISAYDT 60
Query: 66 AWVAMVPSPNSLNKPLFPECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKR 125
AWVAMVPSPNSLNKPLFPECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKR
Sbjct: 61 AWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKR 120
Query: 126 WNIGQDHMSKALSFIKSNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDA 185
W+IG DHMSKALSFIKSNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL LA MNVDA
Sbjct: 121 WDIGDDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDA 180
Query: 186 LVQKKELELRR--SNSEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAF 245
LVQKKELELR SNSEGGKAYLAYVSEGIGKLQDWEMVM+YQRKNGSLF+SP+TTAVAF
Sbjct: 181 LVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWEMVMRYQRKNGSLFSSPSTTAVAF 240
Query: 246 MHRNDDGCFNYLRSVLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDE 305
MHRNDDGCFNYLRSVLQKF SSVP IYPLDIYARLHMVDSLQKLGID HFK+EI+SVLDE
Sbjct: 241 MHRNDDGCFNYLRSVLQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDGHFKDEIRSVLDE 300
Query: 306 TYRCWMQGEENIFLDASTCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGAS 365
TY CWMQGEENIFLDASTCAMAFRML VEGYDVSSDQLTQFSE LFSNCLGGHLKDF AS
Sbjct: 301 TYSCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEGLFSNCLGGHLKDFSAS 360
Query: 366 LELFKASQLLIRPDESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYN 425
LELFKASQ++I PDE ILEN+NSWTSRFLNHGLSSGSVHSDRT+ LVKQEAVNA EFPYN
Sbjct: 361 LELFKASQIIIYPDEFILENINSWTSRFLNHGLSSGSVHSDRTERLVKQEAVNAFEFPYN 420
Query: 426 ATLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELE 485
+TLERLSNKRALESYSGDIVRISKT YACLNFGHQDFLELAVEDFNTLQ IHRKELKELE
Sbjct: 421 STLERLSNKRALESYSGDIVRISKTAYACLNFGHQDFLELAVEDFNTLQGIHRKELKELE 480
Query: 486 KWVVENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS 545
KWV+ENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS
Sbjct: 481 KWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS 540
Query: 546 EEELVNLIQLVEKWDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDI 605
EEELVNLIQLVEKWDA+GETGYCSKEVEIIFL LHST+CEIG +AL WQGRSVMRNVIDI
Sbjct: 541 EEELVNLIQLVEKWDASGETGYCSKEVEIIFLALHSTICEIGKKALPWQGRSVMRNVIDI 600
Query: 606 WLALLESMRKEAEWLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCE 665
WLALLESMRKEAEWLK KVVPS+DEYMENGYVSFALGPIVLP LYF+GPKLPEE++GNCE
Sbjct: 601 WLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIVGNCE 660
Query: 666 YKKLFKLMSTCGRLLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERT 725
YKKLFKLMST GRLLNDTRT DRESSEGKLNA+SL MISAGGK KEEATEAMKGDV+RT
Sbjct: 661 YKKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYMISAGGKLTKEEATEAMKGDVDRT 720
Query: 726 RRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLD 785
RRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKALFEQPMVLD
Sbjct: 721 RRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFEQPMVLD 780
Query: 786 ELLNK 789
ELLNK
Sbjct: 781 ELLNK 785
BLAST of HG10005272.1 vs. ExPASy TrEMBL
Match:
A0A5D3BE57 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00380 PE=4 SV=1)
HSP 1 Score: 1418.3 bits (3670), Expect = 0.0e+00
Identity = 700/793 (88.27%), Postives = 734/793 (92.56%), Query Frame = 0
Query: 23 ASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPLF 82
+SLFPGLDVDTKTKTGALRFE+TKERI++LF VELSVSAYDTAWVAMVPSPNSLN+PLF
Sbjct: 92 SSLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNRPLF 151
Query: 83 PECIDWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSK------- 142
PECI+WVLD+Q+PDGSWGILHDHQ + KATLLSTLAC+LTLKRWNIG DHMSK
Sbjct: 152 PECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKGMLTFLE 211
Query: 143 ------------------ALSFIKSNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLS 202
ALSFIKSNIASATDE QRSPVGFDIIFPGMIEY KDLNLNL
Sbjct: 212 FFFMKYSVMNSRHFLCFAALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLP 271
Query: 203 LAPMNVDALVQKKELELRR--SNSEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNS 262
LA MNVDALVQKKELELR SNSEGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+S
Sbjct: 272 LASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSS 331
Query: 263 PATTAVAFMHRNDDGCFNYLRSVLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKE 322
P+TTAVAFMHRNDDGCFNYLRS+LQKF SSVP IYPLDIYARLHMVDSLQKLGIDRHFK+
Sbjct: 332 PSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKD 391
Query: 323 EIKSVLDETYRCWMQGEENIFLDASTCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGG 382
EI+SVLDETYRCWMQGEENIFLDASTCAMAFRML VEGYDVSSDQLTQFSEDLF NCLGG
Sbjct: 392 EIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG 451
Query: 383 HLKDFGASLELFKASQLLIRPDESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAV 442
HLKDFGASLELFKASQ++I PDE ILEN+NSWTSRFLNHGLS GSVH DRT+ LVKQEAV
Sbjct: 452 HLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHFDRTERLVKQEAV 511
Query: 443 NALEFPYNATLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIH 502
NA EFPYN+TLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIH
Sbjct: 512 NAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIH 571
Query: 503 RKELKELEKWVVENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVD 562
RKELKELEKWV+ENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVD
Sbjct: 572 RKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVD 631
Query: 563 DFFDVGGSEEELVNLIQLVEKWDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRS 622
DFFDVGGSEEELVNLIQLVEKWDA+GE GYCSKEVEIIFL LHST+CEIG +AL WQGRS
Sbjct: 632 DFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRS 691
Query: 623 VMRNVIDIWLALLESMRKEAEWLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLP 682
V RNVIDIWLALLESMRKEAEWLK KVVPS+DEYMENGYVSFALGPIVLP LYF+GPKLP
Sbjct: 692 VTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLP 751
Query: 683 EEVIGNCEYKKLFKLMSTCGRLLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEA 742
EE+IGNCEY+KLFKLMST GRLLNDTRT DRESSEGKLNA+SL +ISAGGK +KEEATEA
Sbjct: 752 EEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEEATEA 811
Query: 743 MKGDVERTRRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKAL 789
MKGDV+RTRRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELM+SAKAL
Sbjct: 812 MKGDVDRTRRELLRLVLQENSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKAL 871
BLAST of HG10005272.1 vs. TAIR 10
Match:
AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 839.3 bits (2167), Expect = 2.5e-243
Identity = 428/770 (55.58%), Postives = 563/770 (73.12%), Query Frame = 0
Query: 22 NASLFPGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPL 81
+A+L LD + +T+ + FE+TKE+IR++ +KVELSVSAYDT+WVAMVPSP+S N PL
Sbjct: 15 SATLERRLDSEVQTRANNVSFEQTKEKIRKMLEKVELSVSAYDTSWVAMVPSPSSQNAPL 74
Query: 82 FPECIDWVLDNQHPDGSWGI-LHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFI 141
FP+C+ W+LDNQH DGSWG+ HDHQ LKK L STLA IL LK+W IG+ ++K L FI
Sbjct: 75 FPQCVKWLLDNQHEDGSWGLDNHDHQSLKKDVLSSTLASILALKKWGIGERQINKGLQFI 134
Query: 142 KSNIASATDEKQRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSN-- 201
+ N A TDE + P GFDIIFPGMI+Y +DLNL + L VD +++K++L+L+ +
Sbjct: 135 ELNSALVTDETIQKPTGFDIIFPGMIKYARDLNLTIPLGSEVVDDMIRKRDLDLKCDSEK 194
Query: 202 -SEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRS 261
S+G +AYLAYV EG L+DW+++++YQRKNGSLF+SPATTA AF +DGC YL S
Sbjct: 195 FSKGREAYLAYVLEGTRNLKDWDLIVKYQRKNGSLFDSPATTAAAFTQFGNDGCLRYLCS 254
Query: 262 VLQKFDSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIFL 321
+LQKF+++VP++YP D YARL ++ +L+ LGIDR FK EIKS+LDETYR W++G+E I L
Sbjct: 255 LLQKFEAAVPSVYPFDQYARLSIIVTLESLGIDRDFKTEIKSILDETYRYWLRGDEEICL 314
Query: 322 DASTCAMAFRMLCVEGYDVSSDQLTQFSEDL-FSNCLGGHLKDFGASLELFKASQLLIRP 381
D +TCA+AFR+L GYDVS D L F+E+ FS+ L G++K+ + LELFKA+Q P
Sbjct: 315 DLATCALAFRLLLAHGYDVSYDPLKPFAEESGFSDTLEGYVKNTFSVLELFKAAQSY--P 374
Query: 382 DESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRALE 441
ES L+ WT ++L LSS V + D +K+E +AL FP A+LER ++R +
Sbjct: 375 HESALKKQCCWTKQYLEMELSSW-VKTSVRDKYLKKEVEDALAFPSYASLERSDHRRKIL 434
Query: 442 SYSG-DIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDKLK 501
+ S + R++KT+Y N D L+LAV+DFN Q IHR+E++ L++W+VEN+L +LK
Sbjct: 435 NGSAVENTRVTKTSYRLHNICTSDILKLAVDDFNFCQSIHREEMERLDRWIVENRLQELK 494
Query: 502 FARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVE 561
FARQKLAYCYFS AATL SPEL DAR+SWAK GVLTTVVDDFFDVGGS+EEL NLI LVE
Sbjct: 495 FARQKLAYCYFSGAATLFSPELSDARISWAKGGVLTTVVDDFFDVGGSKEELENLIHLVE 554
Query: 562 KWDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEA 621
KWD NG Y S+ VEIIF L T+ E G++A +QGR+V +++ IWL LL+SM +EA
Sbjct: 555 KWDLNGVPEYSSEHVEIIFSVLRDTILETGDKAFTYQGRNVTHHIVKIWLDLLKSMLREA 614
Query: 622 EWLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCG 681
EW K PS+++YMEN Y+SFALGPIVLPA Y IGP LPE+ + + +Y +L+KL+ST G
Sbjct: 615 EWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQYNQLYKLVSTMG 674
Query: 682 RLLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQE- 741
RLLND + RES+EGKLNAVSL M +KE E+MKG ER R EL +LVL+E
Sbjct: 675 RLLNDIQGFKRESAEGKLNAVSLHMKHERDNRSKEVIIESMKGLAERKREELHKLVLEEK 734
Query: 742 NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMSSAKALFEQPMVLDE 785
S +PR CK+ F KMS V++LFYRKDDGFTS++LMS K++ +P+ L E
Sbjct: 735 GSVVPRECKEAFLKMSKVLNLFYRKDDGFTSNDLMSLVKSVIYEPVSLQE 781
BLAST of HG10005272.1 vs. TAIR 10
Match:
AT1G61120.1 (terpene synthase 04 )
HSP 1 Score: 287.3 bits (734), Expect = 3.6e-77
Identity = 222/774 (28.68%), Postives = 371/774 (47.93%), Query Frame = 0
Query: 58 LSVSAYDTAWVAMVPSPNSLN----KPLFPECIDWVLDNQHP-DGSWGILHDHQFLKKA- 117
+S SAYDTAW++M+ +++ KP+F C+DW++ NQ+ +G W ++ + A
Sbjct: 36 VSPSAYDTAWLSMIEEDINVDDNELKPMFQGCLDWIMCNQNAREGFW--MNSTSYTTVAD 95
Query: 118 -----------TLLSTLACILTLKRWNIGQDHMSKALSFIKSN----IASATDEKQRSPV 177
L STLAC++ L++WNIG H+ K +I+ N I +E+ P
Sbjct: 96 GRDEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPR 155
Query: 178 GFDIIFPGMIEYVKDLNLNLSLAPMNVDAL----VQKKELELRRS--NSEGGKAYLAYVS 237
F I F G++E + L L+ + ++ + Q++E+ R + K LAY+
Sbjct: 156 WFAIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLE 215
Query: 238 EGIGKL----QDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQKFDSSV 297
KL Q+ +V +GSLF SP+ TA AFM + C YL++++QK + V
Sbjct: 216 VLPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGV 275
Query: 298 PTIYPL-DIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGEENIF--LDASTCA 357
P YPL + +L MV+ ++ G+ F EI+ VL++ Y + EE F + S A
Sbjct: 276 PQKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRY---EEKDFERMPMSYLA 335
Query: 358 -------MAFRMLCVEGYDVSSDQLTQFSEDLFS-NCLGGHLKDF-GASLELFKASQLLI 417
+AFRML + G DVS F D + N L ++ F L +++A+ L+
Sbjct: 336 DQLHKDSLAFRMLRMHGRDVSPRSFCWFLNDQETRNHLERNIDSFLLVILSVYRATDLMF 395
Query: 418 RPDESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVKQEAVNALEFPYNATLERLSNKRA 477
P E L+ +T L ++ ++ ++ ++ L P+ A L+ L ++
Sbjct: 396 -PGEHDLQEAREYTRNLL-----------EKRRSIKEKMIMHELSTPWIARLKHLDHRMW 455
Query: 478 LESYSGDIVRISKTTYACLNFGHQDFL-ELAVEDFNTLQCIHRKELKELEKWVVENKLDK 537
+E + +++ + K ++ L+ + D L LA +F Q + +EL+EL WV + L
Sbjct: 456 IEDKNSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSD 515
Query: 538 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 597
+ F R+K YCYF+ +L AK +L T+ DDFFD GS +L L +
Sbjct: 516 IGFGREKTTYCYFATVTSLPYEYAIKFGKLAAKTAILITIADDFFDEKGSFNDLEGLTKA 575
Query: 598 VEKWDANGETGYCSKEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRK 657
V +W+ Y + IIF L V E N ++ ++ +IW ES +
Sbjct: 576 VLRWEGEELKSYGN----IIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLR 635
Query: 658 EAEWLKTKVVPSMDEYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMST 717
EAEW K SMDEY+ NG +S A I L + P P + Y + L+
Sbjct: 636 EAEWSKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMI 695
Query: 718 CGRLLNDTRTVDRESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRRELLRLVLQ 777
RLLND ++ +E +GK+N+V L M + G E++ ++ ++ R+E L VL
Sbjct: 696 IPRLLNDLQSYQKEQEQGKMNSVLLHMKNHPG-LEIEDSIAHIEKIIDSKRKEFLEHVLV 755
Query: 778 EN-STIPRACKDLFWKMSCVVHLFYRKDDGFTSH-ELMSSAKALFEQPMVLDEL 786
+ S +P+ CK++ V +F+ K + + S+ E++ K P+ + EL
Sbjct: 756 DGLSDLPKPCKEIHMSCCKVFEMFFNKKNRYDSNTEMLHDIKKALYDPINVYEL 787
BLAST of HG10005272.1 vs. TAIR 10
Match:
AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 285.4 bits (729), Expect = 1.4e-76
Identity = 173/531 (32.58%), Postives = 274/531 (51.60%), Query Frame = 0
Query: 27 PGLDVDTKTKTGALRFEETKERIRELFDKVELSVSAYDTAWVAMVPSPNSLNKPLFPECI 86
P + V + + + K +R L D E+++SAYDTAWVA++ + + P FP +
Sbjct: 83 PQISVGSNSNAFKEAVKSVKTILRNLTDG-EITISAYDTAWVALIDAGD--KTPAFPSAV 142
Query: 87 DWVLDNQHPDGSWGILHDHQFLKKATLLSTLACILTLKRWNIGQDHMSKALSFIKSNIAS 146
W+ +NQ DGSWG + F L++TLAC++ L+ WN+ +K ++F + NI
Sbjct: 143 KWIAENQLSDGSWG--DAYLFSYHDRLINTLACVVALRSWNLFPHQCNKGITFFRENIGK 202
Query: 147 ATDEK-QRSPVGFDIIFPGMIEYVKDLNLNLSLAPMNVDALVQKKELELRRSNSE---GG 206
DE + P+GF++ FP ++E + +N+++ + + KKEL+L R E
Sbjct: 203 LEDENDEHMPIGFEVAFPSLLEIARGINIDVPYDSPVLKDIYAKKELKLTRIPKEIMHKI 262
Query: 207 KAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPATTAVAFMHRNDDGCFNYLRSVLQKF 266
L + EG+ L DWE +++ Q ++GS SP++TA AFM D C YLR+ +++F
Sbjct: 263 PTTLLHSLEGMRDL-DWEKLLKLQSQDGSFLFSPSSTAFAFMQTRDSNCLEYLRNAVKRF 322
Query: 267 DSSVPTIYPLDIYARLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQ-----GEENIFL 326
+ VP ++P+D++ + +VD LQ+LGI R+F+EEIK LD +R W +
Sbjct: 323 NGGVPNVFPVDLFEHIWIVDRLQRLGISRYFEEEIKECLDYVHRYWTDNGICWARCSHVQ 382
Query: 327 DASTCAMAFRMLCVEGYDVSSDQLTQFSEDLFSNCLGGHLKDFGASL-ELFKASQLLIRP 386
D AMAFR+L GY VS+D F ++ C G + L++ASQL P
Sbjct: 383 DIDDTAMAFRLLRQHGYQVSADVFKNFEKEGEFFCFVGQSNQAVTGMFNLYRASQLAF-P 442
Query: 387 DESILENVNSWTSRFLNHGLSSGSVHSDRTDTLVK--------QEAVNALEFPYNATLER 446
E IL+N ++ +L +R + + K E ALE P+ A+L R
Sbjct: 443 REEILKNAKEFSYNYLLE-------KREREELIDKWIIMKDLPGEIGFALEIPWYASLPR 502
Query: 447 LSNKRALESYSGD-IVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVV 506
+ + ++ Y G+ V I KT Y + +LELA +D+N Q H+ E +KW
Sbjct: 503 VETRFYIDQYGGENDVWIGKTLYRMPYVNNNGYLELAKQDYNNCQAQHQLEWDIFQKWYE 562
Query: 507 ENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFF 539
EN+L + R +L CY+ AAAT+ E R+ WAK+ VL + F
Sbjct: 563 ENRLSEWGVRRSELLECYYLAAATIFESERSHERMVWAKSSVLVKAISSSF 599
BLAST of HG10005272.1 vs. TAIR 10
Match:
AT4G16740.1 (terpene synthase 03 )
HSP 1 Score: 164.9 bits (416), Expect = 2.7e-40
Identity = 122/424 (28.77%), Postives = 210/424 (49.53%), Query Frame = 0
Query: 277 RLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQG-----EENIFLDASTCAMAFRMLCV 336
+L ++D+LQ+LG+ HF++EIK L + ++ + N + D A+ FR+L
Sbjct: 77 QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136
Query: 337 EGYDVSSDQLTQFSEDLFSNCLGGHL--KDFGASLELFKASQLLIRPDESILENVNSWTS 396
G+ + ++D+F +G L KD L L++AS L R D + E++ T
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196
Query: 397 RFLNHGLSSGSVHSDRTDT-LVKQEAVNALEFPYNATLERLSNKRALESYSGDIVRISKT 456
R V+ + T + +++ ++ALE PY+ + RL + +E Y G+ ++
Sbjct: 197 RLRKF----VEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVY-GERHDMNPI 256
Query: 457 TYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDK-LKFARQKLAYCYFS 516
LELA DFN +Q IH+ ELK L W + L K L F R ++ YFS
Sbjct: 257 -----------LLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFS 316
Query: 517 AAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANGETGYCS 576
+ + PE R K +L T +DD +D+ G+ EEL +VEKWD N
Sbjct: 317 SVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVN-RLEELP 376
Query: 577 KEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTKVVPSMD 636
+++ FL L + + +IG L +G +V+ + + W + + KEA+W K+ P+ +
Sbjct: 377 NYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFE 436
Query: 637 EYMENGYVSFALGPIVLPALYFIGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDTRTVDRE 692
EYM+NG++S ++ P +L L+ + +++G+ + + + +T RL ND T E
Sbjct: 437 EYMQNGWISSSV-PTILLHLFCLLSDQTLDILGSYNH-SVVRSSATILRLANDLATSSEE 473
BLAST of HG10005272.1 vs. TAIR 10
Match:
AT4G16730.1 (terpene synthase 02 )
HSP 1 Score: 157.1 bits (396), Expect = 5.6e-38
Identity = 138/502 (27.49%), Postives = 222/502 (44.22%), Query Frame = 0
Query: 277 RLHMVDSLQKLGIDRHFKEEIKSVLDETYRCWMQGE-ENIFLDASTCAMAFRMLCVEGYD 336
+L M+DSLQ+LGI H+K EI +L + + GE E D ++ F +L G+D
Sbjct: 44 QLEMIDSLQRLGISYHYKHEIHDILRKIHD--QHGEIERETQDLHATSLEFILLRQHGFD 103
Query: 337 VSSDQLTQFSEDL--FSNCLGGHLKDFGASLELFKASQLLIRPDESILENVNSWTSRFLN 396
VS D F + F L +K L L++AS + + + E T + N
Sbjct: 104 VSQDAFDVFISETGEFRKTLHSDIKGL---LSLYEASYFSMDSEFKLKE-----TRIYAN 163
Query: 397 HGLSSGSVHSDRT-----DTLVKQEAVNALEFPYNATLERLSNKRALESYSGDIVRISKT 456
LS S +T +T + + ALE PY+ ++ RL + + Y K
Sbjct: 164 KRLSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRLEARWYINVYE------KKH 223
Query: 457 TYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDK-LKFARQKLAYCYFS 516
L LE A DFN LQ H++ELK + W L K L F R ++ YF
Sbjct: 224 EMNPL------LLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFW 283
Query: 517 AAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDANGETGYCS 576
PE R K +L ++DD +D+ G+ EEL +VEKWD N
Sbjct: 284 TIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVN-HVERLP 343
Query: 577 KEVEIIFLTLHSTVCEIGNRALAWQGRSVMRNVIDIWLALLESMRKEAEWLKTKVVPSMD 636
+ + FL L++ + +IG L +G +V+ + +W L ++ E++W KT PS +
Sbjct: 344 NYMRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGHKPSFE 403
Query: 637 EYMENGYVSFALGPIVLPALYFIGPKLPEEVI-GNCEYKKLFKLMSTCGRLLNDTRTVDR 696
EYM+NG +S ++ I+L + + ++ + + + + + +T RL ND T
Sbjct: 404 EYMQNGVISSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILRLANDLATSTE 463
Query: 697 ESSEGKLNAVSLCMISAGGKFNKEEATEAMKGDVERTRREL-LRLVLQENSTIPRACKDL 756
E + G C + + ++EEA M+ + + + L S++PR
Sbjct: 464 EMARGDSPKSVQCYMYE-TRASEEEARRHMQSMISDSWDIINSDLKTAHTSSLPRGFLAA 521
Query: 757 FWKMSCVVHLFYRKDDGFTSHE 768
++ VV YR DG S E
Sbjct: 524 AANLNRVVQCIYRHGDGHGSPE 521
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038888073.1 | 0.0e+00 | 93.22 | ent-kaur-16-ene synthase, chloroplastic isoform X2 [Benincasa hispida] | [more] |
| XP_038888074.1 | 0.0e+00 | 92.98 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida] | [more] |
| XP_038888071.1 | 0.0e+00 | 92.98 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_03888... | [more] |
| XP_016900364.1 | 0.0e+00 | 91.66 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo] | [more] |
| XP_008447073.1 | 0.0e+00 | 91.42 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q39548 | 0.0e+00 | 81.66 | Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1 | [more] |
| A0A2K9RFY0 | 1.4e-251 | 55.76 | Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ... | [more] |
| A0A1Z3GC64 | 1.6e-247 | 55.32 | Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... | [more] |
| A0A0U4CDK4 | 7.5e-245 | 53.97 | Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS P... | [more] |
| A0A075FBT3 | 1.7e-244 | 56.12 | Ent-kaurene synthase, chloroplastic (Fragment) OS=Marrubium vulgare OX=41230 GN=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S4DWJ8 | 0.0e+00 | 91.66 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... | [more] |
| A0A1S3BG06 | 0.0e+00 | 91.42 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... | [more] |
| A0A5A7V5B3 | 0.0e+00 | 91.15 | Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
| Q9FRX5 | 0.0e+00 | 89.81 | Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1 | [more] |
| A0A5D3BE57 | 0.0e+00 | 88.27 | Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... | [more] |
