HG10000565.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000565.1
TypemRNA
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationChr09: 6492245 .. 6494382 (+)
Sequence length1566
RNA-Seq ExpressionHG10000565.1
SyntenyHG10000565.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCCAAAGTTTTCCCCTTTACATATCCATGCTTCTGGGTTTGCTGGCGATTATATCATCCTACGGGAGTTGCAGCTTTGACACTGACTTCCCCATTGAAGAAGCAACTGTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAGGCAACTTGTCGAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGATCAAGCCTCCATAGCCGACAACGAGCGCAAGAGAAACTCGCTGAGCACTTTGTCTCCATTGCACGGCATTCCTGTACTTCTAAAAGATAACATTGCAACCAGGGATAAGCTCAATACAACGGCTGGCTCTTTTGCTTTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGCGAGTGGTCTGATTTCAGGTCCTATGAACCACCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGAAAGGTGTGAAACTTTTCCAACTTCTTCAATAATTGTCTTCTTGTAAGTGAAATCACATAAGAAAGAATCTGTACTACAGAGTAGGATTGTCACTTGTCAGAATATATCTCTAATAATAGGATTAAAGAAGATTTTGAAACTGTTAGACATACTCAGATTCAGCCAATAAACAATCCCTTTAACTAAAAATATCTAGTTAACATCAGCCTCCAAGCTAACTTTTAAAACAAAACCAGTCAGAAAGTCCAATTGAAAGACGGCAGAAAAAAAAAAGTTTTAAATCAGAAACAAACTAAGAGCACCCCGTATGCACCCTGTTTGCAGAATCCTTACACAATGGGAGAACCTTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCCGCCAATATGGTTACGGTTTCACTGGGAACTGAAACCGATGGATCAATATTATGCCCTTCAACTCTCAATTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTCCCGATTTCTTTGAGGCAGGACACTGTCGGGTAATCAATTTGTTTCTATTCAAATGTCGCCATTTATTCTTGATATCCAAAGGAAGTACTCTCTGCTGAATGAGTAACGATGGAACAGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAACTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGCAAGGAAAGAGAATAGGAATCGTGAGGAAATTCTATGATTTTGGTCATGATGATGCCTTCTACCCTGGAGCTTTTGAGAAAGTTTTCAAAACCCTGAAGTAAGAAAGCAAACTCACTTTTCATAAACCATTTCTTGACAATTCATGTTAGAGGTTTTTATCTGACCCTTTGTCTTCAGTAACATGAAAGTCAAGGAGGAGCAGTATTGGTGGACAATCTGACGATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGAGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGTGAACAATTATCATCTAGTCTGTTCTAATTTTGCAATCAGCATAGCAAAAGGCCGCCTATCATAACAATGAAATCTTATTACTATGTAGGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAGGCCACAAAAGGAATCGGAGATGCAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGACGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCGGCTGGATATAACCCTCAAGGGGTTCCCTGTGGCAATGGCTTCGGAGGATTAAAAGGGTTCGAACCAAGGCTGATAGAGATTGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCCCCTTCACTTGGGAGACGTTAA

mRNA sequence

ATGGCTGCCCAAAGTTTTCCCCTTTACATATCCATGCTTCTGGGTTTGCTGGCGATTATATCATCCTACGGGAGTTGCAGCTTTGACACTGACTTCCCCATTGAAGAAGCAACTGTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAGGCAACTTGTCGAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGATCAAGCCTCCATAGCCGACAACGAGCGCAAGAGAAACTCGCTGAGCACTTTGTCTCCATTGCACGGCATTCCTGTACTTCTAAAAGATAACATTGCAACCAGGGATAAGCTCAATACAACGGCTGGCTCTTTTGCTTTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGCGAGTGGTCTGATTTCAGGTCCTATGAACCACCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGAAAGAATCCTTACACAATGGGAGAACCTTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCCGCCAATATGGTTACGGTTTCACTGGGAACTGAAACCGATGGATCAATATTATGCCCTTCAACTCTCAATTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTCCCGATTTCTTTGAGGCAGGACACTGTCGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAACTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGCAAGGAAAGAGAATAGGAATCGTGAGGAAATTCTATGATTTTGGTCATGATGATGCCTTCTACCCTGGAGCTTTTGAGAAAGTTTTCAAAACCCTGAATCAAGGAGGAGCAGTATTGGTGGACAATCTGACGATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGAGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGTGAACAATTATCATCTAGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAGGCCACAAAAGGAATCGGAGATGCAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGACGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCGGCTGGATATAACCCTCAAGGGGTTCCCTGTGGCAATGGCTTCGGAGGATTAAAAGGGTTCGAACCAAGGCTGATAGAGATTGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCCCCTTCACTTGGGAGACGTTAA

Coding sequence (CDS)

ATGGCTGCCCAAAGTTTTCCCCTTTACATATCCATGCTTCTGGGTTTGCTGGCGATTATATCATCCTACGGGAGTTGCAGCTTTGACACTGACTTCCCCATTGAAGAAGCAACTGTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAGGCAACTTGTCGAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGATCAAGCCTCCATAGCCGACAACGAGCGCAAGAGAAACTCGCTGAGCACTTTGTCTCCATTGCACGGCATTCCTGTACTTCTAAAAGATAACATTGCAACCAGGGATAAGCTCAATACAACGGCTGGCTCTTTTGCTTTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGGAAAGCGAGCCTGAGCGAGTGGTCTGATTTCAGGTCCTATGAACCACCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGAAAGAATCCTTACACAATGGGAGAACCTTGTGGCTCAAGTAGCGGCTCTGCCATATCCGTTGCCGCCAATATGGTTACGGTTTCACTGGGAACTGAAACCGATGGATCAATATTATGCCCTTCAACTCTCAATTCAGTAGTTGGCATTAAACCCACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTCCCGATTTCTTTGAGGCAGGACACTGTCGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAACTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGCAAGGAAAGAGAATAGGAATCGTGAGGAAATTCTATGATTTTGGTCATGATGATGCCTTCTACCCTGGAGCTTTTGAGAAAGTTTTCAAAACCCTGAATCAAGGAGGAGCAGTATTGGTGGACAATCTGACGATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGAGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGTGAACAATTATCATCTAGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAGAAGCAGAGGCCACAAAAGGAATCGGAGATGCAGAAAAGGCAGCATTAGCCAGATTAGCAAAACTGTCAAAAGACGGATTTGAGAGATTGATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTCGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCGGCTGGATATAACCCTCAAGGGGTTCCCTGTGGCAATGGCTTCGGAGGATTAAAAGGGTTCGAACCAAGGCTGATAGAGATTGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCCCCTTCACTTGGGAGACGTTAA

Protein sequence

MAAQSFPLYISMLLGLLAIISSYGSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGRR
Homology
BLAST of HG10000565.1 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 892.9 bits (2306), Expect = 1.3e-255
Identity = 463/522 (88.70%), Postives = 485/522 (92.91%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVE 60
           MA QSFP+YISMLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQDTVGPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWT 360
           +GKRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEA 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEA
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEA 420

Query: 421 TKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           NPQG+P G GFGGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000565.1 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 460/520 (88.46%), Postives = 482/520 (92.69%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSYGSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFY 60
           MAAQSFP+YISMLLGLLAI+SSYGSCSFDT+F IEEAT+KDLQLAFYQNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKL 120
           L+QVRRFNPIL GIIEVNPDAL+QAS AD ERKR+S  +LSPLHGIPVL+KDNIAT+D+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYE PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQG 300
           VPISLRQDTVGPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ D L+G
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTAL 360
           KRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI S  +I G  SGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATK 420
            AEFKIS N YLK+LVASPIRSLSDAIEFN+KNSK      LEKLKEYGQELFLEAEATK
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSK------LEKLKEYGQELFLEAEATK 420

Query: 421 GIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNP
Sbjct: 421 GIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNP 480

Query: 481 QGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           QG+P G GFGGLKGFEPRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 QGLPFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of HG10000565.1 vs. NCBI nr
Match: XP_008457662.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 877.5 bits (2266), Expect = 5.8e-251
Identity = 459/522 (87.93%), Postives = 481/522 (92.15%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVE 60
           MA QSFP+YISMLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQDTVG    TV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWT 360
           +GKRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEA 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEA
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEA 420

Query: 421 TKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           NPQG+P G GFGGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of HG10000565.1 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 875.5 bits (2261), Expect = 2.2e-250
Identity = 454/511 (88.85%), Postives = 475/511 (92.95%), Query Frame = 0

Query: 12  MLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNP 71
           MLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVEFYL+QVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFAL 131
           ILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+DKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQGKNPYTMGEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 251
           GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 VGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQGKRIGIVRKF 311
           VGPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L+GKRIGIVRK 
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 YDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTALPAEFKISFN 371
           YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWTAL AEFKIS N
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATKGIGDAEKAA 431
           AYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEATKGIG AEKAA
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEATKGIGGAEKAA 420

Query: 432 LARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPCGNGF 491
           LARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P G GF
Sbjct: 421 LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGF 480

Query: 492 GGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           GGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 GGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of HG10000565.1 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 865.5 bits (2235), Expect = 2.3e-247
Identity = 452/520 (86.92%), Postives = 477/520 (91.73%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSYGSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFY 60
           MAAQSF LYISM+L L+AI+SSYG+CSF T FPIEEATVKDLQLAFYQNKLTSRQLV+FY
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSFGTHFPIEEATVKDLQLAFYQNKLTSRQLVKFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKL 120
           LKQVRRFNPILKGIIEVNPDALDQA+ AD+ERKR+SLS+LSPLHGIPVLLKDNIAT+DKL
Sbjct: 61  LKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSPLHGIPVLLKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRS   PNGW ARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRS-NLPNGWCARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQG 300
           VPIS RQD VGPICRTVSDAAYVLDAIVGAD+YDNSTIEASKYIPKGGYGQFLR D L+G
Sbjct: 241 VPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLKG 300

Query: 301 KRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTAL 360
            RIGIVRKF+DFG D  FY  A+EKVFK+L QGGA+LVDNLTI+SLDIIIGGLSGEWTAL
Sbjct: 301 TRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGLSGEWTAL 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATK 420
           PAEFKIS NAYLKELVASPIRSLSDAIEFNKKNSK      LEKLKEYGQ LFLEAEAT 
Sbjct: 361 PAEFKISINAYLKELVASPIRSLSDAIEFNKKNSK------LEKLKEYGQVLFLEAEATN 420

Query: 421 GIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GIGD EKAALA +A LSK+GFER+MIKNKLDAIAAPG LISPVLAIGGFPG+SVPAGY+P
Sbjct: 421 GIGDVEKAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSP 480

Query: 481 QGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
            GVP G GFGGLKGFEPRLIEIAYGFE LTKSRK P + R
Sbjct: 481 DGVPYGIGFGGLKGFEPRLIEIAYGFEQLTKSRKPPLIKR 513

BLAST of HG10000565.1 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.0e-157
Identity = 303/524 (57.82%), Postives = 392/524 (74.81%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLL---AIISSYGSCS---FDTDFPIEEATVKDLQLAFYQNKLTSR 60
           M A  F  + S+L+ L+   A+I S GS S     + F I+EAT++D+++AF + +LTS+
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVEFYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNI 120
           QLVE YL+ + + NPIL  +IE NPDAL QA IAD ER   + + L  LHG+PVLLKD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 ATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSA 180
           +T+DKLNTTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW+ FRS+  P+GWSA
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RGGQGKNPYTM-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVG 240
           RG QGKNPY +   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VG
Sbjct: 181 RGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVG 240

Query: 241 LTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFL 300
           LTSRAGVVPISLRQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL
Sbjct: 241 LTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFL 300

Query: 301 RADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGL 360
               L+GKR+GIV K          +    +   KTL + GA++++NLTI ++++I+GG 
Sbjct: 301 TTSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGT 360

Query: 361 -SGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQEL 420
            SGE  AL AEFK+S NAYLKELV SP+RSL+D I +N++ ++       EK+KE+GQE+
Sbjct: 361 DSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAE------QEKVKEWGQEV 420

Query: 421 FLEAEATKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGV 480
           FL AEAT G+G+ EK AL ++ +LS++G E+L+ +NKLDAI   G  +S VLAIGG+PG+
Sbjct: 421 FLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGI 480

Query: 481 SVPAGYNPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSP 517
           +VPAGY+  GVP G  FGGL+  EP+LIEIA+ FE  T  RK P
Sbjct: 481 NVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of HG10000565.1 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.7e-78
Identity = 196/509 (38.51%), Postives = 286/509 (56.19%), Query Frame = 0

Query: 34  IEEATVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEVNPDALDQASIADNERK 93
           +E+AT+  LQ       LTS  +V  YL +  + NP + GI+++NPD L  AS  D+ER 
Sbjct: 66  LEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDERA 125

Query: 94  RNSLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFG 153
              +    PLHGIP ++KDN AT+DK++TTAGS+ALLGSIVPRDA VV +LR+AGA++FG
Sbjct: 126 NGIIR--GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFG 185

Query: 154 KASLSEWSDFRSYEPPNGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGTET 213
            A+LSEW+D RS +   G+SARGGQ + P+ +   P GSSSGSAISVA+NM+  +LGTET
Sbjct: 186 HATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTET 245

Query: 214 DGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADR 273
           DGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV  ++ G D 
Sbjct: 246 DGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGIDE 305

Query: 274 YDNSTIEASKYIPK-GGYGQFL-RADRLQGKRIGIV-RKFYDFGHDDAFYPGAFEKVFKT 333
            D  T+  +   P+ G Y +FL     L+G R G+  ++ +     D        +V K 
Sbjct: 306 NDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEI--DRLLEVVKQ 365

Query: 334 LNQGGAVLVDNLTIESLDII--------IGGLS-GEWTALPAEFKISFNAYLKELVASPI 393
           + + GA++ +N    +LD+I        +G ++  E+T +  +F  +  +YL E+  + I
Sbjct: 366 IEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTEI 425

Query: 394 RSLSDAIEFNKKNSKLVNNY-HLEKLKEYGQELFLEAEATKGI-GDAEKAALARLAKLSK 453
            SL D +E+N K         ++      GQ+ FL +    G+  +    A+  + + S+
Sbjct: 426 HSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTSQ 485

Query: 454 D-GFERLM---IKNKLDAIAAPGRLISPVLAI-------GGFPGVSVPAGYNPQGVPCGN 513
           D G +  +        D+    G L+    +I        G+P +++P G    G P G 
Sbjct: 486 DEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFGL 545

Query: 514 GFGGLKGFEPRLIEIAYGFEHLTKSRKSP 517
           G       EP+LI+     E L + +  P
Sbjct: 546 GIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of HG10000565.1 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.0e-66
Identity = 178/501 (35.53%), Postives = 261/501 (52.10%), Query Frame = 0

Query: 42  LQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLS 101
           LQ  + Q  +    +V+ Y+ ++   N  ++ + E+NPDAL  A   DNERK   L    
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR--G 153

Query: 102 PLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS 161
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR+AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 162 DFRSYEPPNGWSARGGQGKNPYTMG-EPCGSSSGSAISVAANMVTVSLGTETDGSILCPS 221
           +FRS    NGWSA GGQ    Y    +P GSSSGS ++    +   +LGTET GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 222 TLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEA 281
             +++VG+KPTVGLTSR  VVPIS RQDTVGP+ R+V DAAY+L  I G D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 282 SKYIPKGGYGQFLRADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDN 341
             +     Y +    + L+GKRIG+ R               F +    + + GA++V+N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 342 LTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNY 401
               S          +   L A+   +  A+ K+L  +P  +++D      ++ +    +
Sbjct: 394 TDFTSFAEFAQSPIPD-DILYADSLTNLPAFFKQLKVNP-HNITDL-----ESLRRFTQH 453

Query: 402 HLEKLKEYGQE--LFLEAEATKGIGDAEKAALARLAKLSKDGFERLMI----KNKLDAIA 461
           H  +L+EY        +    KGI + +        K  K G E  ++    ++KLDA  
Sbjct: 454 H--RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAV 513

Query: 462 APGRLISPVLAIGGFPGVSVPAGYNPQ---------------GVPCGNGFGGLKGFEPRL 521
            P  L   + A+ G P ++VP G  P                G+P G GF G    E +L
Sbjct: 514 LPTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKL 573

BLAST of HG10000565.1 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.1e-42
Identity = 153/510 (30.00%), Postives = 253/510 (49.61%), Query Frame = 0

Query: 38  TVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEVNPD-ALDQASIADNERKRNS 97
           TV +L+   ++ +++++++ + YL++++   P +  ++ +  + AL++A +AD   K   
Sbjct: 7   TVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADEMIKNGE 66

Query: 98  LSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKAS 157
              L+   GIPV++KDNI+T + + TT  S  L   I P +A VV +L   G +I GK++
Sbjct: 67  AKDLT---GIPVIIKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVVILGKSN 126

Query: 158 LSEWSDFRSYEPPNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGS 217
           L E++   S E     ++     KNP+ +   P GSS GSA +VAA+    +LG++T GS
Sbjct: 127 LDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALGSDTGGS 186

Query: 218 ILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDN 277
           I  P++L  VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+AI G D  D+
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAGHDPMDS 246

Query: 278 STIEASKYIPKGGYGQFLRADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGA 337
           ++++    I K  Y  +L+ D ++G RIG+ ++F+  G ++       EK  K     GA
Sbjct: 247 TSVK----IEKPDYTSYLKED-IKGLRIGVAKEFFGAGIEEG-VKETVEKAIKVFEDLGA 306

Query: 338 VLVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFN---KK 397
            ++D +++  ++           ALPA + I+         AS   +  D I +    K 
Sbjct: 307 EIID-ISVPYVEY----------ALPAYYIIA------SAEASSNLARYDGIRYGHIAKN 366

Query: 398 NSKLVNNYHLEKLKEYGQE----LFLEAEA-TKGIGDAEKAALARLAKLSKDGFERLMIK 457
              LV+ Y   + + +G+E    + L   A + G  DA      ++  L K+ FER    
Sbjct: 367 YEDLVDMYMTSRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFERAF-- 426

Query: 458 NKLDAIAAP---------GRLISPVLA------------IGGFPGVSVPAGYNPQGVPCG 517
            K D I  P         G   +  LA            I G P +S+P G + +G+P G
Sbjct: 427 EKCDVIVGPTSPTVAFKIGERTNDPLAMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVG 481

BLAST of HG10000565.1 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 7.1e-42
Identity = 157/510 (30.78%), Postives = 256/510 (50.20%), Query Frame = 0

Query: 38  TVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEVNPD-ALDQASIADNERKRNS 97
           T+ +L+    + ++++ ++ + YL++++   P +  ++ +  D AL +A  AD + K+  
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGE 66

Query: 98  LSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKAS 157
            + L+   GIPV++KDNI+T + + TT  S  L   I P +A VV KL + G II GK++
Sbjct: 67  DTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 158 LSEWSDFRSYEPPNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGS 217
           L E++   S E     ++     KNP+ +   P GSS GSA ++AA+    +LG++T GS
Sbjct: 127 LDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGS 186

Query: 218 ILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDN 277
           I  P++L  VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I+G D  D+
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDS 246

Query: 278 STIEASKYIPKGGYGQFLRADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGA 337
           ++++    I K  Y  +L+ D ++G RIG+ ++F        F  G  E V +T+ +   
Sbjct: 247 TSLK----IDKPDYTSYLKED-IKGLRIGVAKEF--------FGEGIEEGVKETVQESIK 306

Query: 338 VLVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFN---KK 397
           VL D L  E +DI I  +     ALPA + I+         AS   +  D I +    +K
Sbjct: 307 VLQD-LGAEIIDISIPYVE---YALPAYYIIA------SAEASSNLARYDGIRYGHIAEK 366

Query: 398 NSKLVNNYHLEKLKEYGQE----LFLEAEA-TKGIGDAEKAALARLAKLSKDGFERLMIK 457
              L++ Y + + + +G+E    + L   A + G  DA      ++  L K+ FE+    
Sbjct: 367 YEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF-- 426

Query: 458 NKLDAIAAP---------GRLISPVLA------------IGGFPGVSVPAGYNPQGVPCG 517
            K D I  P         G   +  LA            I G PG+S+P G +  G+P G
Sbjct: 427 EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVG 481

BLAST of HG10000565.1 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 6.5e-256
Identity = 463/522 (88.70%), Postives = 485/522 (92.91%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVE 60
           MA QSFP+YISMLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQDTVGPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWT 360
           +GKRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEA 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEA
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEA 420

Query: 421 TKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           NPQG+P G GFGGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000565.1 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 6.5e-256
Identity = 463/522 (88.70%), Postives = 485/522 (92.91%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVE 60
           MA QSFP+YISMLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQDTVGPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWT 360
           +GKRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEA 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEA
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEA 420

Query: 421 TKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           NPQG+P G GFGGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000565.1 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.6e-254
Identity = 459/520 (88.27%), Postives = 481/520 (92.50%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSYGSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFY 60
           MAAQSFP+YISMLLGLLAI+SSYGSCSFDT+F IEEAT+KDLQLAFYQNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKL 120
           L+QVRRFNPIL GIIEVNPDAL+QAS AD ERKR+S  +LSPLHGIPV +KDNIAT+D+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYE PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQG 300
           VPISLRQDTVGPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ D L+G
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTAL 360
           KRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI S  +I G  SGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATK 420
            AEFKIS N YLK+LVASPIRSLSDAIEFN+KNSK      LEKLKEYGQELFLEAEATK
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSK------LEKLKEYGQELFLEAEATK 420

Query: 421 GIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNP
Sbjct: 421 GIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNP 480

Query: 481 QGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           QG+P G GFGGLKGFEPRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 QGLPFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of HG10000565.1 vs. ExPASy TrEMBL
Match: A0A1S3C652 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.8e-251
Identity = 459/522 (87.93%), Postives = 481/522 (92.15%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVE 60
           MA QSFP+YISMLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQDTVG    TV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWT 360
           +GKRIGIVRK YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEA 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEA
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEA 420

Query: 421 TKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGIG AEKAALARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           NPQG+P G GFGGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of HG10000565.1 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.1e-250
Identity = 454/511 (88.85%), Postives = 475/511 (92.95%), Query Frame = 0

Query: 12  MLLGLLAIISSY--GSCSFDTDFPIEEATVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNP 71
           MLLGLLAI+S Y  GSCSFDT+F IEEAT+KD QLAFYQNKLTSRQLVEFYL+QVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFAL 131
           ILKGIIEVNPDAL+QAS AD +RKR+SL +LSPLHGIPVL+KDNIAT+DKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGGQGKNPYTMGEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 251
           GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 VGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLQGKRIGIVRKF 311
           VGPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L+GKRIGIVRK 
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 YDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTALPAEFKISFN 371
           YDFGHDD FY GAFEKVFKTL QGGA+LVDNLTI   D+I G  SGEWTAL AEFKIS N
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATKGIGDAEKAA 431
           AYLK+LVASPIRSLSDAIEFNKKNSK      LEKL+EYGQELFLEAEATKGIG AEKAA
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSK------LEKLREYGQELFLEAEATKGIGGAEKAA 420

Query: 432 LARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPCGNGF 491
           LARLAKLSK+GFERLMIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P G GF
Sbjct: 421 LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGF 480

Query: 492 GGLKGFEPRLIEIAYGFEHLTKSRKSPSLGR 521
           GGLKGF+PRLIEIAYGFEHLT  RKSPSLGR
Sbjct: 481 GGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of HG10000565.1 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 478.8 bits (1231), Expect = 5.6e-135
Identity = 270/523 (51.63%), Postives = 355/523 (67.88%), Query Frame = 0

Query: 1   MAAQSFPLYISMLLGLL---AIISSYGSCS---FDTDFPIEEATVKDLQLAFYQNKLTSR 60
           M A  F  + S+L+ L+   A+I S GS S     + F I+EAT++D+++AF + +LTS+
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVEFYLKQVRRFNPILKGIIEVNPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNI 120
           QLVE YL+ + + NPIL  +IE NPDAL QA IAD ER   + + L  LHG+PVLLKD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 ATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSA 180
           +T+DKLNTTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW+ FRS+  P+GWSA
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240
                                                       PS  NSVVGIKP+VGL
Sbjct: 181 --------------------------------------------PSQ-NSVVGIKPSVGL 240

Query: 241 TSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLR 300
           TSRAGVVPISLRQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL 
Sbjct: 241 TSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLT 300

Query: 301 ADRLQGKRIGIVRKFYDFGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGL- 360
              L+GKR+GIV K          +    +   KTL + GA++++NLTI ++++I+GG  
Sbjct: 301 TSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTD 360

Query: 361 SGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELF 420
           SGE  AL AEFK+S NAYLKELV SP+RSL+D I +N++ ++       EK+KE+GQE+F
Sbjct: 361 SGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAE------QEKVKEWGQEVF 420

Query: 421 LEAEATKGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVS 480
           L AEAT G+G+ EK AL ++ +LS++G E+L+ +NKLDAI   G  +S VLAIGG+PG++
Sbjct: 421 LTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGIN 462

Query: 481 VPAGYNPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTKSRKSP 517
           VPAGY+  GVP G  FGGL+  EP+LIEIA+ FE  T  RK P
Sbjct: 481 VPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of HG10000565.1 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 9.2e-29
Identity = 137/529 (25.90%), Postives = 237/529 (44.80%), Query Frame = 0

Query: 19  IISSYGSCSFDTD-FPIEEATVKDLQLAFYQNKLTSRQLVEFYLKQVRRFNPILKGIIEV 78
           I+S+  S + DT      ++ +   + +    + T+ ++ + YL ++R   P LK  + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 79  NPDALDQASIADNERKRNSLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRD 138
           + + L  A   D    +     L PL G+ + +KDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDAQEIDQRIAKG--EELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 139 AGVVTKLRKAGAIIFGKASLSEWSDFRSYEPPNGWSARGGQGKNPYTMGE-PCGSSSGSA 198
           A  V K+++ G I+ GK ++ E+    + E     ++      NP+ +   P GSS GSA
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEFGMGSTTE-----ASAFQVTANPWDLSRVPGGSSGGSA 204

Query: 199 ISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRT 258
            +VAA    VSLG++T GS+  P++   VVG+KPT G  SR G++  +   D +G    T
Sbjct: 205 AAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGST 264

Query: 259 VSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADR-----LQGKRIGIVRKFYD 318
           V+DA  +L AI G DR+D++   +SK        QFL  D      L G ++GI+R+  +
Sbjct: 265 VADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLE 324

Query: 319 FGHDDAFYPGAFEKVFKTLNQGGAVLVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAY 378
            G D      A ++    L   G +L + +++ S  +           LPA + I+ +  
Sbjct: 325 DGVDSG-VRSATQEAASHLEALGCILTE-VSLPSFSL----------GLPAYYVIASSES 384

Query: 379 LKELVASPIRSLSDAIEFNKK--NSKLVNNYHLEKLKEYGQELFLEA-----EATKGIGD 438
              L      S  D + +  +    +L   Y   + + +G E+ +         + G  D
Sbjct: 385 SSNL------SRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYD 444

Query: 439 AEKAALARLAKLSKDGFERLMIKNK-LDAIAAP------------------GRLISPVLA 498
           A      ++  L +  F+  + +N  L + AAP                  G +++  + 
Sbjct: 445 AYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVN 504

Query: 499 IGGFPGVSVPAGY---NPQGVPCGNGFGGLKGFEPRLIEIAYGFEHLTK 512
           + G P + +P G     P G+P G    G    E +L+++ + FE   K
Sbjct: 505 LAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of HG10000565.1 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 7.8e-28
Identity = 84/232 (36.21%), Postives = 128/232 (55.17%), Query Frame = 0

Query: 50  KLTSRQLVEFYLKQVRRFNPILKGIIEVNPD-ALDQASIADNERKRNSLSTLSPLHGIPV 109
           ++TS++LV  YLKQ++R+N +L+ ++    + A  QA  AD+   + +   L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY--LGPLHGIPY 275

Query: 110 LLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEP 169
            LKD +A      TT GS +     +  +A V  +L+ +GA++  K          S   
Sbjct: 276 GLKDIVAV-PGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAY 335

Query: 170 PNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
            + W   GG+ +NP+ + E   GSS+G A S +A MV  ++G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of HG10000565.1 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-24
Identity = 82/232 (35.34%), Postives = 124/232 (53.45%), Query Frame = 0

Query: 50  KLTSRQLVEFYLKQVRRFNPILKGIIEVNPD-ALDQASIADNERKRNSLSTLSPLHGIPV 109
           ++TS++LV  YLKQ++R+N +L+ ++    + A  QA  AD+   + +   L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTY--LGPLHGIPY 275

Query: 110 LLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEP 169
            LKD +A      TT GS +     +  +A V  +L+ +GA++  K          S   
Sbjct: 276 GLKDIVAV-PGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAY 335

Query: 170 PNGWSARGGQGKNPYTMGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVG 229
            + W   GG+ +NP+ + E   GSS+G A S +A       G+ET GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 230 IKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDNSTIE 280
           ++PT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of HG10000565.1 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-19
Identity = 123/500 (24.60%), Postives = 200/500 (40.00%), Query Frame = 0

Query: 39  VKDLQLAFYQNKLTSRQLVEFYLKQVRRF---NPILKGIIEVNPDALDQASIADNERKRN 98
           ++D   A+     T  Q+ +  +  +  F    P    +I  + + + + + A   R   
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQ 190

Query: 99  SLSTLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKA 158
             + +S L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GKA
Sbjct: 191 G-NPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKA 250

Query: 159 SLSEWSDFRSYEPPNGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGS 218
           ++ E     +    N  + R       YT     GSSSGSA  VAA + + +LGT+  GS
Sbjct: 251 NMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAIVAAGLCSAALGTDGGGS 310

Query: 219 ILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADRYDN 278
           +  PS L  + G+K T G T   G +      + +GP+  ++ DA  V  AI+G+   D 
Sbjct: 311 VRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADR 370

Query: 279 STIEAS-----KYIPKGGYGQFLRADRLQGKRIGIVRK-FYDFGHDDAFYPGAFEKVFKT 338
             ++ S     K +   G      ++ +   R+G   K F D    D       E + K 
Sbjct: 371 YNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSD--ISDKCEDILKL 430

Query: 339 LNQGGAVLVDNLTIESLD-------IIIGG--LSG--EWTALPAEFKISFNAYLKELVAS 398
           L+      V  + +  L+       I IG   LS    +       K+S++      +  
Sbjct: 431 LSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFAIFR 490

Query: 399 PIRSLSDAIEFNKKNSKLVNNYHLEKLKEYGQELFLEAEATKGIGDAEKAALARLAKLSK 458
              S SD I       +L+  YHL   K+      ++   T   G     A        K
Sbjct: 491 SF-SASDYIAAQCLRRRLM-EYHLNIFKD------VDVIVTPTTG---MTAPVIPPDALK 550

Query: 459 DGFERLMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPCGNGFGGLKGFEPR 518
           +G   + +   L        L+       GFP +SVP GY+ +G+P G    G    E  
Sbjct: 551 NGETNIQVTTDLMRFVLAANLL-------GFPAISVPVGYDKEGLPIGLQIMGRPWAEAT 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008457659.11.3e-25588.70PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
XP_004147024.31.1e-25488.46probable amidase At4g34880 [Cucumis sativus][more]
XP_008457662.15.8e-25187.93PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo][more]
ADN34007.12.2e-25088.85amidase [Cucumis melo subsp. melo][more]
XP_038900913.12.3e-24786.92probable amidase At4g34880 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A1P8B7601.0e-15757.82Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY44.7e-7838.51Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C87.0e-6635.53Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
Q8RC401.1e-4230.00Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus s... [more]
B0K3S37.1e-4230.78Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
Match NameE-valueIdentityDescription
A0A5D3BN386.5e-25688.70Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C7A86.5e-25688.70putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
A0A0A0LLK01.6e-25488.27Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=... [more]
A0A1S3C6522.8e-25187.93putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
E5GC081.1e-25088.85Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34880.15.6e-13551.63Amidase family protein [more]
AT3G25660.19.2e-2925.90Amidase family protein [more]
AT5G07360.17.8e-2836.21Amidase family protein [more]
AT5G07360.21.1e-2435.34Amidase family protein [more]
AT5G64440.11.7e-1924.60fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 56..456
e-value: 3.5E-67
score: 227.4
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 24..518
e-value: 1.6E-138
score: 464.8
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 33..514
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 19..518
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 19..518

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
HG10000565HG10000565gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
HG10000565.1-cdsHG10000565.1-cds-Chr09:6492245..6492784CDS
HG10000565.1-cdsHG10000565.1-cds-Chr09:6493106..6493317CDS
HG10000565.1-cdsHG10000565.1-cds-Chr09:6493406..6493645CDS
HG10000565.1-cdsHG10000565.1-cds-Chr09:6493737..6493950CDS
HG10000565.1-cdsHG10000565.1-cds-Chr09:6494023..6494382CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
HG10000565.1HG10000565.1-proteinpolypeptide