Cucsat.G9278.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G9278.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRINT1-like protein MAG2
Locationctg1658: 432500 .. 438110 (-)
RNA-Seq ExpressionCucsat.G9278.T4
SyntenyCucsat.G9278.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAAAATGTTAATTTCTTCTAAGGGCCTTTTCTTCGTTGTTCATTTCTACTACTCCGTCTCTCTCTTTGGGAAGAAGCGGATTACAGTACAACGGAATGTGATCATCTTCTTAGTTAAATTCGATTCATCTTCAATTTTCTCAATCTATGGAAGAGAAATTGATTTGTAAATCTTAGAATCGACATTGATTCATACAGAATTTGGCTCTCACAATCCTCACTCCATTGCCCTCAATCATGAGTGAGCTCGAGCTCGAGCTCAAAGCTCTTCCTCCTCCTTCAAATCTTTCGCCCTCAGTCCTTTCGTTCCTCGATCATCAGCTATACAACAAAGAAACTCTAGCGCAAGCCCCGACTCTCGTCATCGACCTTCAAAGTCAGTGCCATGAATTGAGTCACACTCTAATCGACCTCAATCGGAGTCTTAAACATACTCTTCTTTCTCAGTCTACGTTTTCGGATCGCCTTCATGGCCTTCTTGGAGATGTCAATGGAAAATTGATGGGTCTTGAATCTCTTACTCGCTCTCAGAGTTCCACGCAAGGTTTGTGCAGTTTGCTTTCTTGTCGAATTATACGCGTTGTTGTCGTTAGTTGTATGGTGTTTATGAAATGGGAAATTGTTGTGTATACTCATGCCCTGTTATGTAACTTTTTTTTTCCTTTTTTGCAATTGAGTACGAGATCTCTGCTTGTTGATCTGTTACAGGAGTGGGGATAGCTGATGGGGTGTTGGGGAAGGAGCTGTCGTCGCTCGCAAAAGAAGTGGCGAGAATGGAGACCGTGCGGATGTATGCCGGTGAGTTGCTGTTGCTATTCTCCCTTATTTTCTTCTCGGAGTATAATTAATGTAGTCGGGGGAAGGCGTTGAAATGTTGAGCTGGGGATATGATTCTATGCCGATCATGAATGACCATTTTTGTATCTTAGATTGAAAAAGTTGTAATTTGAAAAGATTTTTATGGGGTTATTTGAAACTATGTGTACTTTGTTGTGTTAATCATTACCTTTTTATTATTGGTCTTTTCTAAACATATCAATGAAAATTAAATTCTAGAAGAGAAACTCATTCTTAGTGCTTCCTGGGTGCTCCTCGCTCAAAGATTGCGGGAGAATGGCAACCATCTTTTCTGATCTAGTCCCAAGGTGATCGATACATGGGATAAGTTTCGGAGGGTTTTTTAATGTGACCAAGGTTCTACGTTCTTTGGTAAAGTTGATGGCCTTTAAAATCCTTTTGGACTTTCATTTTAATATTCAGTATAAAAACCTTGATCAATGTTGGAAGATGCTCTTAGGGTTCATTTGGTTGAAGTGAAGTAGAAGAATTTGTAGTGAAGGAGGTCTTGCCCTTGCAATTAGCTTTAGAACGATGCAATTTATCTTATGACTCTTTGAGCTCTTAAATCAAGTTTTGTCTCCTGTTATTGTTGTCATTCTTTTTTTTGTTTTGTTGACCAATTGGAGAGCCTTTTTTAAATGCCTTGGTTGGTGTTTATTCTTGTGAAGCCTTATTCTTCAAGAATATATTAACTAGAATTATTGTTTTACCTTATATGTGTGTGGCTAGAAGGAAATTTTTTTTGACAACAAGAAACAAGACATAGCGATTTGTTCTTTTAGCCGAATGTGCAACTGAATTGCACACCCTACATATATATTTTTTGAATAGAAAATAAGTTAATTGATGAGATGGAATGATACAAAAGAGAGATTTCCCAAGTGATTTTAGAAAGCTTGTCCAATTTGATAGGAGACTATTTAAGCTGTAGTTGTTGAGAGGGACTAAAGTTTTAACCCAAAAAAGAGCCATAAATAGAGCCAACATGAGTTTAGCTCAATTGAAACCTGTTTGTACCTGAGGACAAAAGGTCCCGGGTTCAAACCCCCCCACCTCAAGTTGTACTACAATACCTTTTAAAAAAAAAGTCATAAATAGAGCTACGTCAAGGCTTTCTTGTGGAGAGCTTTCCTTGTCTTGAAAGCTGTGAGGTTACTTTTTTCATATTTTCCATAAGACAGCCCTGATGATGTGATGCCAAAGAATACTACTCTTTGTCCATTTGAATGAATGATCTTTTAAAAGCAAATTAAGCCAATCCATAATCTATGGGGAGCCAATGTTTCATCCAAAAAGACTTCAGCCCAAAATTTTGTTGTGTAGGGGCAGTGAACGAAGATGTGTCTTTGAGATTCACCAGCTTCTTTACAAAGCACCAATTTTGGGAGATGACAGCCCAAAGGCATCTTCACCGGAACCGATTGTAACTTAATTGTTGAGGCAAGAATGGTTGAGTTCCCATTCTCACAAAAAGGAATAAAAGAAGAAATTTTATCTTCTTAGGATAATGCCCCTTCCAAATGTTCTTGTACTGCTCCATGGAGAGAGCATGATTTCCCATCAGTAAGGTTGGAGACTAATGATTTTATAGAGAAAGTGCCCTGATGTGTCTCCAAACTCGTTGATCTTTCCCATGCAAGAGATGAACTGAGTCACTGAAGAAATAAGCTGAGGGGATGCCCATTCTGAAATTTCGTCTTTTTTGAGGTTTCTCCATAAATTTTGAACCTATGAATTGTTCTCCTTGTTCCAAACATCATCAATGGCAGACCTCTAGCAGTAACAGATTTATATGTATAGTCCGGAATCATCAAATTACATCTCTAATTTCTCGTTTTCACTCTCTTACATATTTCAAAATTTGTTTTCCTTTCAAATATTTCAGAAACAACAATGAAGCTTGATAGCATGGTAGGAGATATCGAAGATGCTGTATCTTCTGCAATAAACAAAAACTTGAGGAAGCAGAGTTCAGAGGTGAGGACTTTGCCAACTTTTTGTGTAAAAGAAGGGATGGAAACCCCACTGTTGGCTGGTTTGCAAGATGCCCTTGCACAATATTAATTTTCTTCTCACGATTTTTTTATTCTAATGAGCAAATTATAATTTGATTTTAAACTTCTATTATCGACTATGTGACCAGAGACGCATTCCTTGATTGGGAAGATGTTTAAATTCTTGAAGGCTATTCTTTTTCTGACATTTGTTCAAACTGGATGTTTTTTGTAGGATCTTTTTTCAACTGTTTTTTTCTTCGCTTTTTTTCTATTACTTTGTTGTCACTGTCTGCAAATAGAAGGCTGAGCACTTTTGTACTCTTTCTAACTTTGCAATCATTTATGATTTTTGAAAATTTTGTTTCTAGTCTAAAAGCATATAGGTTGTTTACCTTCATGAGCAGGTGTTATCCTGCATTTTGTATATGATTGAACGGAATTTTCTTTGGGTGTTGATAGGATTGGAGGAGGTTTGGGGATAGGTTCTCTTGTCAAAACTATTGTTTATAGCGTGCCAATTTTACCTGTAGCTTGGGTCCTCTTATTATTTTTCTCAGTTTTGTATTTTGTTGTTCCCTAGAAGGGATGGAAACCTTCTCTGTACCCGTGCATCCTAATGAAACATAGATTTCCCATTTACCCCTTGAATTTCTGTTTTTTAACATCCTTATAACTGACCTTTTGAGTACATTCTTGTCTACTGCAGGATGCAAGGCTGCTTGCTATAAAAACCTTCAAACTTACAGAAGACATCTTAGTGTCGGTTTCAAAAACACGGCCTCAGTGGACACATCTTGTGTCAGCAGTTGACCATAGAGTTGATCGGGCATTAGCTATTTTAAGACCTCAGGCAATTGCAGATCATCGCTCTCTTCTTTCTTCCCTTGGATGGCCTCCACCTCTCTCCACTGTAACGGTTACAGGTGATGCAACAAAATCGACTGAATCCCAAAATCCTCTCTTTACAATGCAAGGAAAGCTCAAACAGCAATACTGTGAAAATTTTCTTGCCCTATGCAGCCTGCAAGAGATTCAAAGACGAAGGAAATCTCGACAACTTGAAGGTTACAGTAAAGAGGTTTCTTTGCCACAGCCCCTTTGGGCAATTGAAGAGCTTGTAAATCCTATATCATTAGCAGCTCAAGAACATTTTTCAAAGTGGATTGATAAGCCAGAATTCATTTTTATTCTCACTTACAAGATTACCAGGGATTATGTTGACTCTATAGACGAAGTTTTGCAACCTCTTGTTGATGAAGCCAGGTTGGTGGGGTATAGTTGCAGAGAAGAATGGATATCATCAATGGTAACCTCCTTGTCAACTTACTTAGCAAAGGAGATTTTCCCAAATTACGTCCGTCAACTCGATGAAGACTCCAACATTGGTATTCAATCACAGGCTAGAATATCATGGCTTCATCTAGTCGACCTGATGATATCTTTTGATAAAAGAATTAAATCACTTGTTGAGCAGTCTGGACTTTTGCTTTCTTTTGATGAAAATGGGAACTTGCAAAGACTTTCTTCACTAGCTGTCTTTTGTGATAGACCTGATTGGCTTGATTTATGGGCAGAAATGGAGCGAAGTGATGCGATGCTTAAACTACAAATTGAGGTAGATAATGAGAGGAATTGGTCTGATAAGATTCCAGCTGCAGCTCTTCCTTCTTCCTCTGAGCATTCTAAATCTCCTGCAATTTCCACCGTCTTTATTAAGCACCTCTCGTCTTTGGTTTATCGCTGTCAATCTCTACCCAGTATCACATTGAGGTCAAGATTTTTCAAGCTAGCTGGTTCTCCTATCATAGCTAATGTTTTTAATTGTGTACTAATCAGGTGCCAGGAAGCTGAAGGTTTAACTGCCTTGACAGACGATGATGCATTGGTTAAAGTTGCAAACTCTATTAATGCTGCACGATATTTTGAATCTATTCTAAAAGAATGGTGTGAAGACATGTTTTTCCTCGAGATGGGAACAGCTTCTGATGAGCTGTTAGCAAGTCCATCAACTGGTATTATCGACTCGGAGATTAGAAAGTTTGAAGAGTTTAGACGCGAATGGGTTGAGAAAATATCCACGGTTATTCTGAGGGGTTTTGACGCTCAAAGTAGAGATTACATAAAGAACAAAAAACAATGGAAGGAAAAATTTGAAGATGGTTGGACAGTGTCCAGGCTTTTAATTGGAGCTTTAGATTATTTGCAAGGAAAAATGTTAACTTTAGAAAAGAATTTGAATGGAATCGACTTCGTTTCACTCTGGAGAACTTTGGCTGCCGGTGTCGATCGATTTATTTTCAATGGCATACTGATGAGCAATGTGCAGTTTAATAATGATGGAGTAAAGAGGTTTGGTGATGATATGGAAGTTTTGTTTGGGATATTTAGGTCTTGGTGTTTGAGGCCTGAAGGTTTTTTCCCAAAAATAAGTGAGAGCATGAAATTGTTGAAGATGAAAGAAGAGCAACTTAAAAGTAGTTTGGTGGGAGAACAGAGTTGGATGAAGGAAAATGGAGTCAAACATTTGAGTACTAGTGAAGTAGACAGAATTGTGAAGAGTAGGATGCTATAGAAACAGAAAGTTCGATAATACATGTTTGGGACTGATATTTAAAATTTTGTATGGATGCTTTTCATTGTTCAAAATCAATTTTGTGATGAAATGAAAGTAGTGTTTTTAAGTGTAAAATCAAACAAGTTGATCTTGAATGACTTTAGATGTTTTAGAGTGAATCTGCGTATGATATAAGTAATTATGATTGGTTTATTTAT

Coding sequence (CDS)

ATGAAGCTTGATAGCATGGTAGGAGATATCGAAGATGCTGTATCTTCTGCAATAAACAAAAACTTGAGGAAGCAGAGTTCAGAGGATGCAAGGCTGCTTGCTATAAAAACCTTCAAACTTACAGAAGACATCTTAGTGTCGGTTTCAAAAACACGGCCTCAGTGGACACATCTTGTGTCAGCAGTTGACCATAGAGTTGATCGGGCATTAGCTATTTTAAGACCTCAGGCAATTGCAGATCATCGCTCTCTTCTTTCTTCCCTTGGATGGCCTCCACCTCTCTCCACTGTAACGGTTACAGGTGATGCAACAAAATCGACTGAATCCCAAAATCCTCTCTTTACAATGCAAGGAAAGCTCAAACAGCAATACTGTGAAAATTTTCTTGCCCTATGCAGCCTGCAAGAGATTCAAAGACGAAGGAAATCTCGACAACTTGAAGGTTACAGTAAAGAGGTTTCTTTGCCACAGCCCCTTTGGGCAATTGAAGAGCTTGTAAATCCTATATCATTAGCAGCTCAAGAACATTTTTCAAAGTGGATTGATAAGCCAGAATTCATTTTTATTCTCACTTACAAGATTACCAGGGATTATGTTGACTCTATAGACGAAGTTTTGCAACCTCTTGTTGATGAAGCCAGGTTGGTGGGGTATAGTTGCAGAGAAGAATGGATATCATCAATGGTAACCTCCTTGTCAACTTACTTAGCAAAGGAGATTTTCCCAAATTACGTCCGTCAACTCGATGAAGACTCCAACATTGGTATTCAATCACAGGCTAGAATATCATGGCTTCATCTAGTCGACCTGATGATATCTTTTGATAAAAGAATTAAATCACTTGTTGAGCAGTCTGGACTTTTGCTTTCTTTTGATGAAAATGGGAACTTGCAAAGACTTTCTTCACTAGCTGTCTTTTGTGATAGACCTGATTGGCTTGATTTATGGGCAGAAATGGAGCGAAGTGATGCGATGCTTAAACTACAAATTGAGGTAGATAATGAGAGGAATTGGTCTGATAAGATTCCAGCTGCAGCTCTTCCTTCTTCCTCTGAGCATTCTAAATCTCCTGCAATTTCCACCGTCTTTATTAAGCACCTCTCGTCTTTGGTTTATCGCTGTCAATCTCTACCCAGTATCACATTGAGGTCAAGATTTTTCAAGCTAGCTGGTTCTCCTATCATAGCTAATGTTTTTAATTGTGTACTAATCAGGTGCCAGGAAGCTGAAGGTTTAACTGCCTTGACAGACGATGATGCATTGGTTAAAGTTGCAAACTCTATTAATGCTGCACGATATTTTGAATCTATTCTAAAAGAATGGTGTGAAGACATGTTTTTCCTCGAGATGGGAACAGCTTCTGATGAGCTGTTAGCAAGTCCATCAACTGGTATTATCGACTCGGAGATTAGAAAGTTTGAAGAGTTTAGACGCGAATGGGTTGAGAAAATATCCACGGTTATTCTGAGGGGTTTTGACGCTCAAAGTAGAGATTACATAAAGAACAAAAAACAATGGAAGGAAAAATTTGAAGATGGTTGGACAGTGTCCAGGCTTTTAATTGGAGCTTTAGATTATTTGCAAGGAAAAATGTTAACTTTAGAAAAGAATTTGAATGGAATCGACTTCGTTTCACTCTGGAGAACTTTGGCTGCCGGTGTCGATCGATTTATTTTCAATGGCATACTGATGAGCAATGTGCAGTTTAATAATGATGGAGTAAAGAGGTTTGGTGATGATATGGAAGTTTTGTTTGGGATATTTAGGTCTTGGTGTTTGAGGCCTGAAGGTTTTTTCCCAAAAATAAGTGAGAGCATGAAATTGTTGAAGATGAAAGAAGAGCAACTTAAAAGTAGTTTGGTGGGAGAACAGAGTTGGATGAAGGAAAATGGAGTCAAACATTTGAGTACTAGTGAAGTAGACAGAATTGTGAAGAGTAGGATGCTATAG

Protein sequence

MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFPKISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML
Homology
BLAST of Cucsat.G9278.T4 vs. ExPASy Swiss-Prot
Match: Q9STU3 (RINT1-like protein MAG2 OS=Arabidopsis thaliana OX=3702 GN=MAG2 PE=1 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 2.3e-231
Identity = 393/660 (59.55%), Postives = 513/660 (77.73%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSS---EDARLLAIKTFKLTEDILVSVSKTRPQWTH 60
           +KLD++VGDIEDAV S++NKNLR   S   E+ RL AIKT K TE+IL SV+K  P+W  
Sbjct: 136 LKLDTLVGDIEDAVMSSLNKNLRTSRSSGFEEVRLHAIKTLKTTEEILSSVAKRHPRWAR 195

Query: 61  LVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQ 120
           LVSAVDHRVDRALA++RPQAIAD+R+LLSSL WPP LST+T     +KS   QNPLF M+
Sbjct: 196 LVSAVDHRVDRALAMMRPQAIADYRALLSSLRWPPQLSTLTSASLDSKSENVQNPLFNME 255

Query: 121 GKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKE-VSLPQPLWAIEELVNPISLAAQEH 180
           G LK QYC +F ALCSLQ +Q +RKSRQL  +  E V   QPLWAIEELVNP+++A+Q H
Sbjct: 256 GSLKSQYCGSFHALCSLQGLQLQRKSRQLGIHKGENVLFHQPLWAIEELVNPLTVASQRH 315

Query: 181 FSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYL 240
           F+KW +KPEFIF L YKITRDYVDS+DE+LQPLVDEA+L GYSCREEW+S+MV+SLS YL
Sbjct: 316 FTKWSEKPEFIFALVYKITRDYVDSMDELLQPLVDEAKLAGYSCREEWVSAMVSSLSLYL 375

Query: 241 AKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGN 300
            KEIFP YV QLDE +   ++S+A++SWLHL+DLMISFDKR++SLV QSG +LS  E+GN
Sbjct: 376 VKEIFPIYVGQLDEANETDLRSEAKVSWLHLIDLMISFDKRVQSLVSQSG-ILSLQEDGN 435

Query: 301 LQRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKS 360
           L R+SSL+VFCDRPDWLDLWAE+E  + ++K + E+DN+RNW+ K+    + SS+ + + 
Sbjct: 436 LLRISSLSVFCDRPDWLDLWAEIELDERLVKFKEEIDNDRNWTAKVQDELISSSNVY-RP 495

Query: 361 PAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALT 420
           P IS++F++HLSS++ R +S+P++ LR+RF +LA SP I    +C+L+RCQ+A+GLTALT
Sbjct: 496 PIISSIFLQHLSSIIERSKSVPALYLRARFLRLAASPTIHKFLDCLLLRCQDADGLTALT 555

Query: 421 DDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASD--------ELLASPSTGIIDS 480
           +++ L+KV+NSINA  Y ES+L+EW ED+FFLEMGT           E    PS GI   
Sbjct: 556 ENNDLIKVSNSINAGHYIESVLEEWSEDVFFLEMGTGQHDPQEVPGLENFTEPSEGIFGE 615

Query: 481 EIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQ 540
           E  K E+FR EW+ K+S VILRGFDA+ R+YIKN+KQW+EK +  WTVSR L+GALDYLQ
Sbjct: 616 EFEKLEKFRLEWINKLSVVILRGFDARIREYIKNRKQWQEKKDKEWTVSRALVGALDYLQ 675

Query: 541 GKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIF 600
           GK   +E+NLN  DF ++WRTLA+ +D+  FN ILM+NV+F NDGV+R   DMEVL+G+F
Sbjct: 676 GKTSIIEENLNKADFTAMWRTLASEIDKLFFNSILMANVKFTNDGVERLKVDMEVLYGVF 735

Query: 601 RSWCLRPEGFFPKISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRM 649
           R+WC+RPEGFFPK+SE + LLKM+E+Q+K  L     W++EN +++LS +E  ++ KSR+
Sbjct: 736 RTWCVRPEGFFPKLSEGLTLLKMEEKQVKDGLSRGDKWLRENRIRYLSEAEAKKVAKSRV 793

BLAST of Cucsat.G9278.T4 vs. ExPASy Swiss-Prot
Match: Q8GXP1 (RINT1-like protein MAG2L OS=Arabidopsis thaliana OX=3702 GN=MAG2L PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.4e-108
Identity = 239/682 (35.04%), Postives = 367/682 (53.81%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSS-EDARLL------AIKTFKLTEDILVSVSKTRP 60
           +KL+S+VGD+ED+V   I+K  RK S+ +D  L       AIKT    E+IL  V++   
Sbjct: 132 LKLESLVGDLEDSVFHPISK--RKGSTLQDLALKQERFSHAIKTMNEIEEILGDVTRHHS 191

Query: 61  QWTHLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPL 120
           +W  LV +VD RVD++L++LRPQ IADHR+ LSSLGWPP L+T  V      S    NPL
Sbjct: 192 RWRRLVDSVDSRVDKSLSVLRPQIIADHRAFLSSLGWPPKLATSKVEHGEVDSI--PNPL 251

Query: 121 FTMQGKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAA 180
             MQG  K+ Y ++FL LC LQ+   +++ R+    +KE      LWA +ELV P++   
Sbjct: 252 LLMQGDKKESYSQSFLLLCGLQQHNTQKEKRKKLNMTKETD-NDGLWATDELVKPVASRM 311

Query: 181 QEHFSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLS 240
           + HF KW ++PEFIF L YK+TRD+ D +D+ LQPL+D A LV  S +E W+S+MV  LS
Sbjct: 312 EYHFLKWAEQPEFIFELVYKVTRDFADGVDDFLQPLIDRAMLVSCSAKEAWVSAMVQMLS 371

Query: 241 TYLAKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDE 300
            +L K++FP  +    E     ++S+   SW HLVD M++FDKR++S V  +   LS++ 
Sbjct: 372 GFLEKKVFPGLIDMYKEKH---MKSEGISSWFHLVDQMVTFDKRMQSFV-NTDTCLSYEG 431

Query: 301 NGNL--QRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNW------------S 360
           +     Q +S + +FC +P+WL  W ++E  DA  K + ++ NE+ W            S
Sbjct: 432 SSTAFSQGISVMGLFCKKPEWLKTWGKIELKDAYRKSKEDIKNEKAWVIDSERTRLGNES 491

Query: 361 DKIPAAALPSSSEHSKSPAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVF 420
           +   A  + S+ E  K+P ++  F+     L+    SLP+I  R +F +   +  +  +F
Sbjct: 492 NSQSAKYVLSTREDYKAPLVADSFLNRTWRLIDHGLSLPAILPRIQFIRATATRFLWCIF 551

Query: 421 NCVLIRCQEAEGLTALTDDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLA 480
             +L+  ++ +       +D L++    +NAARY ES L+EW +D+ F+EM  A   +  
Sbjct: 552 KILLLEFKKTDLSHYGLSEDTLIQACGPVNAARYLESKLREWSDDLVFVEMWAAETSVKV 611

Query: 481 SPSTGI------IDSEIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKK-QWKEKF-- 540
                +         E++   E    W+ +I TV L  FD    D+  N    W E    
Sbjct: 612 DRKPEVSCQGCFFGEELKSLVELETNWLMEIITVFLHQFDNLCSDHFHNNAVSWDEDVIT 671

Query: 541 -EDGWTVSRLLIGALDYLQGKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQF 600
                TVS+ +  ALD L+  +  L  N+N  DF+ LWR LA G+D ++           
Sbjct: 672 SSSNLTVSQGVAEALDNLRRHLCVLHLNMNPKDFLDLWRNLAEGLDHYVSRKFFSGEPVL 731

Query: 601 NNDGVKRFGDDMEVLFGIFRSWCLRPEGFFPKISESMKLLKMKEEQLKSSLVGEQS---- 647
                 RF  D E L  + + +C+RP  FFP++ E ++LL+M EE+ K+ L G  S    
Sbjct: 732 RRQKFDRFEVDAEALLTVLKPYCVRPGAFFPRVREILRLLRMHEEE-KARLRGALSRSGN 791

BLAST of Cucsat.G9278.T4 vs. ExPASy Swiss-Prot
Match: Q8BZ36 (RAD50-interacting protein 1 OS=Mus musculus OX=10090 GN=Rint1 PE=1 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-20
Identity = 138/629 (21.94%), Postives = 257/629 (40.86%), Query Frame = 0

Query: 12  DAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVSAVDHRVDRALA 71
           + +S  I + L   S  +A  L +   +L  DI +  S      THL+S +   V     
Sbjct: 169 EELSDNIQQYLMTNSVPEAASLLVTMTEL--DIQLQESSC----THLLSFMRATVKFWHK 228

Query: 72  ILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKLKQQYCENFLAL 131
           IL+ +  +D   +L+ L WP            T  T+SQ    +      + Y       
Sbjct: 229 ILKDKLTSDFEEVLAQLHWP-----------FTSHTQSQTVGGSRPAGTPELYSSLDTLF 288

Query: 132 CSLQEIQRR----RKSRQL-EGYSKEVSLPQPLWAIEELVNPISLAAQEHF-----SKWI 191
           C L ++Q       + +QL E Y    S P  L  I+ ++ P+    + HF     +  +
Sbjct: 289 CQLLKLQASDELLTEPKQLPEKYCLPASPPVTL-PIQVMLAPLQKRFRYHFRGSRQTNVM 348

Query: 192 DKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEIF 251
            KPE+          ++   +DE +QP++D+      + R E+   +V  +   LA +I 
Sbjct: 349 SKPEWYLAQVLMWIGNHTQFLDEKIQPILDKVG-SAVNARLEFSRGLVMLILEKLASDI- 408

Query: 252 PNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRLS 311
                 L +DS           + HLVD ++ F++ + ++            +G     +
Sbjct: 409 ---PCLLYDDS----------LFCHLVDEVLLFERELHTV------------HGYPSTFA 468

Query: 312 SLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIST 371
           S             W  +ER  A+ K+   + +E  W  +       S  +  K P  + 
Sbjct: 469 SCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQYKDI---SDVDEMKVPDCAE 528

Query: 372 VFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDAL 431
           VF+  L  +  R ++LP+ + + +F +L     + + F   L +  + E   AL      
Sbjct: 529 VFMTLLLVITDRYKNLPTASRKLQFLELQKD--LVDDFRIRLTQVMKEETRAALG----- 588

Query: 432 VKVANSINAARYFESILKEWCEDMFFLEMGTASDEL--------------LASPSTGIID 491
            +    +NA  Y  ++L +W +++FFL++  A+ E+              LAS  + + D
Sbjct: 589 FRYCAILNAVNYISAVLADWADNVFFLQLQQAALEVFAENNVLSKLQLGQLASMESSVFD 648

Query: 492 SEIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKKQW----KEKFEDGWTVSRLLIGA 551
             I   E  + + + +    + R     ++ Y   K++W     +  +   ++S      
Sbjct: 649 DMINLLERLKLDMLTRQVDHVFREVKDSAKLY--KKERWLSLPSQSEQAVMSLSSSACPL 708

Query: 552 LDYLQGKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDM-E 611
           L  L+ ++L LE+ L    F   W+ LA  +D +I+  I+++N  FN  G  +   DM  
Sbjct: 709 LLTLRDRLLQLEQQLCFSLFRIFWQMLAEKLDLYIYQEIILAN-HFNEGGAAQLQFDMTR 739

BLAST of Cucsat.G9278.T4 vs. ExPASy Swiss-Prot
Match: Q6NUQ1 (RAD50-interacting protein 1 OS=Homo sapiens OX=9606 GN=RINT1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.5e-20
Identity = 136/638 (21.32%), Postives = 260/638 (40.75%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           +K  S + ++ D +   +  N   +++     +A    KL E             THL+ 
Sbjct: 162 LKWISQIEELSDNIQQYLMTNNVPEAASTLVSMAELDIKLQES----------SCTHLLG 221

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWP---PPLSTVTVTGDATKSTESQNPLFTMQ 120
            +   V     IL+ +  +D   +L+ L WP   PP S          + E  + L T+ 
Sbjct: 222 FMRATVKFWHKILKDKLTSDFEEILAQLHWPFIAPPQSQTVGLSRPASAPEIYSYLETL- 281

Query: 121 GKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHF 180
                 +C+  L L +  E+    K    E YS   S P  +  I+ ++ P+    + HF
Sbjct: 282 ------FCQ-LLKLQTSDELLTEPKQLP-EKYSLPAS-PSVILPIQVMLTPLQKRFRYHF 341

Query: 181 -----SKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSL 240
                +  + KPE+          ++ + +DE +QP++D+   +  + R E+   ++  +
Sbjct: 342 RGNRQTNVLSKPEWYLAQVLMWIGNHTEFLDEKIQPILDKVGSL-VNARLEFSRGLMMLV 401

Query: 241 STYLAKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFD 300
              LA +I       L  D N+         + HLVD ++ F++ + S+    G   S  
Sbjct: 402 LEKLATDI-----PCLLYDDNL---------FCHLVDEVLLFERELHSVHGYPGTFASC- 461

Query: 301 ENGNLQRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSE 360
               +  LS    F         W  +ER  A+ K+   + +E  W  +       +  +
Sbjct: 462 ----MHILSEETCF-------QRWLTVERKFALQKMDSMLSSEAAWVSQYKDI---TDVD 521

Query: 361 HSKSPAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGL 420
             K P  +  F+  L  +  R ++LP+ + + +F +L     + + F   L +  + E  
Sbjct: 522 EMKVPDCAETFMTLLLVITDRYKNLPTASRKLQFLELQKD--LVDDFRIRLTQVMKEETR 581

Query: 421 TALTDDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASDEL--------------L 480
            +L       +    +NA  Y  ++L +W +++FFL++  A+ E+              L
Sbjct: 582 ASLG-----FRYCAILNAVNYISTVLADWADNVFFLQLQQAALEVFAENNTLSKLQLGQL 641

Query: 481 ASPSTGIIDSEIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKKQW----KEKFEDGW 540
           AS  + + D  I   E  + + + +    + R     ++ Y   K++W     +  +   
Sbjct: 642 ASMESSVFDDMINLLERLKHDMLTRQVDHVFREVKDAAKLY--KKERWLSLPSQSEQAVM 701

Query: 541 TVSRLLIGALDYLQGKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGV 600
           ++S      L  L+  +L LE+ L    F   W+ L   +D +I+  I+++N  FN  G 
Sbjct: 702 SLSSSACPLLLTLRDHLLQLEQQLCFSLFKIFWQMLVEKLDVYIYQEIILAN-HFNEGGA 739

Query: 601 KRFGDDM-EVLFGIFRSWCLRPEGFFPKISESMKLLKM 612
            +   DM   LF +F  +C RPE +F  I E+  +L +
Sbjct: 762 AQLQFDMTRNLFPLFSHYCKRPENYFKHIKEACIVLNL 739

BLAST of Cucsat.G9278.T4 vs. NCBI nr
Match: XP_011658877.1 (RINT1-like protein MAG2 isoform X2 [Cucumis sativus])

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 644/649 (99.23%), Postives = 646/649 (99.54%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLD MVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 1   MKLDCMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPP LSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPSLSTVTVTGDATKSTESQNPLFTMQGKL 120

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FPNY+RQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 241 FPNYIRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA
Sbjct: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELLASPSTGIIDSEIRKFEEFRREW
Sbjct: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLASPSTGIIDSEIRKFEEFRREW 480

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKISTVILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKMLTLEKNLNG
Sbjct: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML
Sbjct: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649

BLAST of Cucsat.G9278.T4 vs. NCBI nr
Match: XP_004137086.1 (RINT1-like protein MAG2 isoform X1 [Cucumis sativus] >KGN43850.1 hypothetical protein Csa_017176 [Cucumis sativus])

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 644/649 (99.23%), Postives = 646/649 (99.54%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLD MVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 136 MKLDCMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 195

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPP LSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 196 AVDHRVDRALAILRPQAIADHRSLLSSLGWPPSLSTVTVTGDATKSTESQNPLFTMQGKL 255

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 256 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 315

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 316 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 375

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FPNY+RQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 376 FPNYIRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 435

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 436 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 495

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA
Sbjct: 496 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 555

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELLASPSTGIIDSEIRKFEEFRREW
Sbjct: 556 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLASPSTGIIDSEIRKFEEFRREW 615

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKISTVILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKMLTLEKNLNG
Sbjct: 616 VEKISTVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 675

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 676 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 735

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML
Sbjct: 736 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 784

BLAST of Cucsat.G9278.T4 vs. NCBI nr
Match: XP_008455035.1 (PREDICTED: RINT1-like protein MAG2 isoform X2 [Cucumis melo])

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLDSMVGDIEDAVSSAINKNLRKQS EDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 1   MKLDSMVGDIEDAVSSAINKNLRKQSLEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FP+Y+ QLDEDSN G+QSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 241 FPSYISQLDEDSNFGVQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEME SDAM+KLQ EVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 301 SSLAVFCDRPDWLDLWAEMELSDAMVKLQTEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           T+FIKHLSSLVYRCQSLPSI+LRSRFFKLAGSPIIAN FNCVLIRCQEAEGLTALTDDDA
Sbjct: 361 TIFIKHLSSLVYRCQSLPSISLRSRFFKLAGSPIIANFFNCVLIRCQEAEGLTALTDDDA 420

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELL SPSTGIIDSEIRKFEEFRREW
Sbjct: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLESPSTGIIDSEIRKFEEFRREW 480

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKIS VILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKM TLEKNLNG
Sbjct: 481 VEKISMVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMSTLEKNLNG 540

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRF+FN ILMSNVQFN+DGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 541 IDFVSLWRTLAAGVDRFLFNCILMSNVQFNDDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLST+EVDRIVKSRML
Sbjct: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTAEVDRIVKSRML 649

BLAST of Cucsat.G9278.T4 vs. NCBI nr
Match: XP_008455034.1 (PREDICTED: RINT1-like protein MAG2 isoform X1 [Cucumis melo] >KAA0031366.1 RINT1-like protein MAG2 isoform X1 [Cucumis melo var. makuwa] >TYK06818.1 RINT1-like protein MAG2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLDSMVGDIEDAVSSAINKNLRKQS EDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 134 MKLDSMVGDIEDAVSSAINKNLRKQSLEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 193

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 194 AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 253

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 254 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 313

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 314 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 373

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FP+Y+ QLDEDSN G+QSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 374 FPSYISQLDEDSNFGVQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 433

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEME SDAM+KLQ EVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 434 SSLAVFCDRPDWLDLWAEMELSDAMVKLQTEVDNERNWSDKIPAAALPSSSEHSKSPAIS 493

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           T+FIKHLSSLVYRCQSLPSI+LRSRFFKLAGSPIIAN FNCVLIRCQEAEGLTALTDDDA
Sbjct: 494 TIFIKHLSSLVYRCQSLPSISLRSRFFKLAGSPIIANFFNCVLIRCQEAEGLTALTDDDA 553

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELL SPSTGIIDSEIRKFEEFRREW
Sbjct: 554 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLESPSTGIIDSEIRKFEEFRREW 613

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKIS VILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKM TLEKNLNG
Sbjct: 614 VEKISMVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMSTLEKNLNG 673

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRF+FN ILMSNVQFN+DGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 674 IDFVSLWRTLAAGVDRFLFNCILMSNVQFNDDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 733

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLST+EVDRIVKSRML
Sbjct: 734 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTAEVDRIVKSRML 782

BLAST of Cucsat.G9278.T4 vs. NCBI nr
Match: XP_038888330.1 (RINT1-like protein MAG2 [Benincasa hispida])

HSP 1 Score: 1170 bits (3026), Expect = 0.0
Identity = 596/653 (91.27%), Postives = 621/653 (95.10%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRK----QSSEDARLLAIKTFKLTEDILVSVSKTRPQWT 60
           MKLDSMVGDIEDAVSSAINKNLRK    QSSEDARL+AIKT KLTEDILVSVSKTRPQWT
Sbjct: 132 MKLDSMVGDIEDAVSSAINKNLRKYSPAQSSEDARLVAIKTLKLTEDILVSVSKTRPQWT 191

Query: 61  HLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTM 120
           HLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTM
Sbjct: 192 HLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTM 251

Query: 121 QGKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEH 180
           QGKLKQQYCENFLALCSLQE+QRRRKSRQLEGYSKE+SL QPLWAIEELVNPISLAAQ+H
Sbjct: 252 QGKLKQQYCENFLALCSLQELQRRRKSRQLEGYSKELSLQQPLWAIEELVNPISLAAQDH 311

Query: 181 FSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYL 240
           FSKWIDKPEFIFILTYKITRDYVDSIDEVLQPL DEARLVGYSCREEWISSMV+SLSTYL
Sbjct: 312 FSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLADEARLVGYSCREEWISSMVSSLSTYL 371

Query: 241 AKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGN 300
           AKEIFPNY+ +LDEDS+ GIQSQARIS LHLVDLMISFDKRIKSLVEQSGLLLSFD + N
Sbjct: 372 AKEIFPNYISRLDEDSDTGIQSQARISLLHLVDLMISFDKRIKSLVEQSGLLLSFDGSEN 431

Query: 301 LQRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKS 360
           LQR+SSLAVFCDRPDWLDLWAEME SDA+ KLQ EVDNE NWSDKIPAAALPSSSEHSKS
Sbjct: 432 LQRISSLAVFCDRPDWLDLWAEMELSDALAKLQTEVDNEGNWSDKIPAAALPSSSEHSKS 491

Query: 361 PAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALT 420
           PAIS VFIK LSSLVYRC+SLPSI+LRSRFFKLAGSPIIA   +CVLIRCQEAEGLTALT
Sbjct: 492 PAISAVFIKQLSSLVYRCRSLPSISLRSRFFKLAGSPIIAKFLSCVLIRCQEAEGLTALT 551

Query: 421 DDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEF 480
           DDDALVKV+NSINAARYFESILKEWCEDMFFLEMG+ASDELL S STGIID EIRKFEEF
Sbjct: 552 DDDALVKVSNSINAARYFESILKEWCEDMFFLEMGSASDELLESQSTGIIDLEIRKFEEF 611

Query: 481 RREWVEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEK 540
           RREWVEKISTVILRGFDA+SRDYIKNKKQWKEK EDGW+VSR LIGALDYLQGKMLTLEK
Sbjct: 612 RREWVEKISTVILRGFDAESRDYIKNKKQWKEKCEDGWSVSRHLIGALDYLQGKMLTLEK 671

Query: 541 NLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPE 600
           NLN IDFVSLWRTLAAG DRF+FNG+LMSNVQFN+ GVKRFG DMEVLFGIFRSWCLRPE
Sbjct: 672 NLNEIDFVSLWRTLAAGTDRFLFNGVLMSNVQFNDVGVKRFGGDMEVLFGIFRSWCLRPE 731

Query: 601 GFFPKISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           GFFPKISESM+LLKMKEEQLKSSLVG Q+WMKEN +KHLST+EVDRIVKSRML
Sbjct: 732 GFFPKISESMRLLKMKEEQLKSSLVGGQTWMKENRLKHLSTTEVDRIVKSRML 784

BLAST of Cucsat.G9278.T4 vs. ExPASy TrEMBL
Match: A0A0A0K2F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071390 PE=4 SV=1)

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 644/649 (99.23%), Postives = 646/649 (99.54%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLD MVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 136 MKLDCMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 195

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPP LSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 196 AVDHRVDRALAILRPQAIADHRSLLSSLGWPPSLSTVTVTGDATKSTESQNPLFTMQGKL 255

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 256 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 315

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 316 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 375

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FPNY+RQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 376 FPNYIRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 435

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 436 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 495

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA
Sbjct: 496 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 555

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELLASPSTGIIDSEIRKFEEFRREW
Sbjct: 556 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLASPSTGIIDSEIRKFEEFRREW 615

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKISTVILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKMLTLEKNLNG
Sbjct: 616 VEKISTVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 675

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 676 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 735

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML
Sbjct: 736 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 784

BLAST of Cucsat.G9278.T4 vs. ExPASy TrEMBL
Match: A0A5D3C6N8 (RINT1-like protein MAG2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001220 PE=4 SV=1)

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLDSMVGDIEDAVSSAINKNLRKQS EDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 134 MKLDSMVGDIEDAVSSAINKNLRKQSLEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 193

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 194 AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 253

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 254 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 313

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 314 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 373

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FP+Y+ QLDEDSN G+QSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 374 FPSYISQLDEDSNFGVQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 433

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEME SDAM+KLQ EVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 434 SSLAVFCDRPDWLDLWAEMELSDAMVKLQTEVDNERNWSDKIPAAALPSSSEHSKSPAIS 493

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           T+FIKHLSSLVYRCQSLPSI+LRSRFFKLAGSPIIAN FNCVLIRCQEAEGLTALTDDDA
Sbjct: 494 TIFIKHLSSLVYRCQSLPSISLRSRFFKLAGSPIIANFFNCVLIRCQEAEGLTALTDDDA 553

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELL SPSTGIIDSEIRKFEEFRREW
Sbjct: 554 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLESPSTGIIDSEIRKFEEFRREW 613

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKIS VILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKM TLEKNLNG
Sbjct: 614 VEKISMVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMSTLEKNLNG 673

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRF+FN ILMSNVQFN+DGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 674 IDFVSLWRTLAAGVDRFLFNCILMSNVQFNDDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 733

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLST+EVDRIVKSRML
Sbjct: 734 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTAEVDRIVKSRML 782

BLAST of Cucsat.G9278.T4 vs. ExPASy TrEMBL
Match: A0A1S3C0P8 (RINT1-like protein MAG2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495307 PE=4 SV=1)

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLDSMVGDIEDAVSSAINKNLRKQS EDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 1   MKLDSMVGDIEDAVSSAINKNLRKQSLEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FP+Y+ QLDEDSN G+QSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 241 FPSYISQLDEDSNFGVQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEME SDAM+KLQ EVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 301 SSLAVFCDRPDWLDLWAEMELSDAMVKLQTEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           T+FIKHLSSLVYRCQSLPSI+LRSRFFKLAGSPIIAN FNCVLIRCQEAEGLTALTDDDA
Sbjct: 361 TIFIKHLSSLVYRCQSLPSISLRSRFFKLAGSPIIANFFNCVLIRCQEAEGLTALTDDDA 420

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELL SPSTGIIDSEIRKFEEFRREW
Sbjct: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLESPSTGIIDSEIRKFEEFRREW 480

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKIS VILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKM TLEKNLNG
Sbjct: 481 VEKISMVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMSTLEKNLNG 540

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRF+FN ILMSNVQFN+DGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 541 IDFVSLWRTLAAGVDRFLFNCILMSNVQFNDDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLST+EVDRIVKSRML
Sbjct: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTAEVDRIVKSRML 649

BLAST of Cucsat.G9278.T4 vs. ExPASy TrEMBL
Match: A0A1S3BZY3 (RINT1-like protein MAG2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495307 PE=4 SV=1)

HSP 1 Score: 1243 bits (3215), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRKQSSEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 60
           MKLDSMVGDIEDAVSSAINKNLRKQS EDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS
Sbjct: 134 MKLDSMVGDIEDAVSSAINKNLRKQSLEDARLLAIKTFKLTEDILVSVSKTRPQWTHLVS 193

Query: 61  AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 120
           AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL
Sbjct: 194 AVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTMQGKL 253

Query: 121 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 180
           KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW
Sbjct: 254 KQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEHFSKW 313

Query: 181 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 240
           IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI
Sbjct: 314 IDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYLAKEI 373

Query: 241 FPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 300
           FP+Y+ QLDEDSN G+QSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL
Sbjct: 374 FPSYISQLDEDSNFGVQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGNLQRL 433

Query: 301 SSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKSPAIS 360
           SSLAVFCDRPDWLDLWAEME SDAM+KLQ EVDNERNWSDKIPAAALPSSSEHSKSPAIS
Sbjct: 434 SSLAVFCDRPDWLDLWAEMELSDAMVKLQTEVDNERNWSDKIPAAALPSSSEHSKSPAIS 493

Query: 361 TVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALTDDDA 420
           T+FIKHLSSLVYRCQSLPSI+LRSRFFKLAGSPIIAN FNCVLIRCQEAEGLTALTDDDA
Sbjct: 494 TIFIKHLSSLVYRCQSLPSISLRSRFFKLAGSPIIANFFNCVLIRCQEAEGLTALTDDDA 553

Query: 421 LVKVANSINAARYFESILKEWCEDMFFLEMGTASDELLASPSTGIIDSEIRKFEEFRREW 480
           LVKVANSINAARYFESILKEWCEDMFFLEMG+ASDELL SPSTGIIDSEIRKFEEFRREW
Sbjct: 554 LVKVANSINAARYFESILKEWCEDMFFLEMGSASDELLESPSTGIIDSEIRKFEEFRREW 613

Query: 481 VEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLIGALDYLQGKMLTLEKNLNG 540
           VEKIS VILRGFDAQSRDYIKNKKQWKEK EDGWTVSRLLIGALDYLQGKM TLEKNLNG
Sbjct: 614 VEKISMVILRGFDAQSRDYIKNKKQWKEKCEDGWTVSRLLIGALDYLQGKMSTLEKNLNG 673

Query: 541 IDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 600
           IDFVSLWRTLAAGVDRF+FN ILMSNVQFN+DGVKRFGDDMEVLFGIFRSWCLRPEGFFP
Sbjct: 674 IDFVSLWRTLAAGVDRFLFNCILMSNVQFNDDGVKRFGDDMEVLFGIFRSWCLRPEGFFP 733

Query: 601 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVDRIVKSRML 649
           KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLST+EVDRIVKSRML
Sbjct: 734 KISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTAEVDRIVKSRML 782

BLAST of Cucsat.G9278.T4 vs. ExPASy TrEMBL
Match: A0A6J1I9Z0 (RINT1-like protein MAG2 OS=Cucurbita maxima OX=3661 GN=LOC111471001 PE=4 SV=1)

HSP 1 Score: 1152 bits (2980), Expect = 0.0
Identity = 582/668 (87.13%), Postives = 619/668 (92.66%), Query Frame = 0

Query: 1   MKLDSMVGDIEDAVSSAINKNLRK----QSSEDARLLAIKTFKLTEDILVSVSKTRPQWT 60
           MKLDSM+GDIEDAVSSAINKNLRK    QSSEDARLLAIKT KLTEDILVSVSKTRPQWT
Sbjct: 132 MKLDSMIGDIEDAVSSAINKNLRKYSPAQSSEDARLLAIKTLKLTEDILVSVSKTRPQWT 191

Query: 61  HLVSAVDHRVDRALAILRPQAIADHRSLLSSLGWPPPLSTVTVTGDATKSTESQNPLFTM 120
           HLVSAVDHRVDRALAILRPQAIADHRSLL+SLGWPPPLS+VTV GDATKSTESQNPLFTM
Sbjct: 192 HLVSAVDHRVDRALAILRPQAIADHRSLLASLGWPPPLSSVTVAGDATKSTESQNPLFTM 251

Query: 121 QGKLKQQYCENFLALCSLQEIQRRRKSRQLEGYSKEVSLPQPLWAIEELVNPISLAAQEH 180
           QGKLKQQYCENFLALCSLQE+QRRRKSRQLEGY++EVSL QPLWAIEELVNPISLAAQ H
Sbjct: 252 QGKLKQQYCENFLALCSLQELQRRRKSRQLEGYNREVSLQQPLWAIEELVNPISLAAQGH 311

Query: 181 FSKWIDKPEFIFILTYKITRDYVDSIDEVLQPLVDEARLVGYSCREEWISSMVTSLSTYL 240
           FSKW+DKPEFIF+LTYKITRDY+DS+DEVLQPLVDEARLVGYSCREEWISS+V+SLSTYL
Sbjct: 312 FSKWVDKPEFIFVLTYKITRDYIDSMDEVLQPLVDEARLVGYSCREEWISSIVSSLSTYL 371

Query: 241 AKEIFPNYVRQLDEDSNIGIQSQARISWLHLVDLMISFDKRIKSLVEQSGLLLSFDENGN 300
           AKEIFPNY+ QLDEDS+ GIQSQARI+WLHLVDLMISFDKRIKSLVEQSGLLLSFDEN N
Sbjct: 372 AKEIFPNYISQLDEDSDTGIQSQARIAWLHLVDLMISFDKRIKSLVEQSGLLLSFDENEN 431

Query: 301 LQRLSSLAVFCDRPDWLDLWAEMERSDAMLKLQIEVDNERNWSDKIPAAALPSSSEHSKS 360
           +QR+SSLA+FCDRPDWLDLWAEME SDA+ KLQ+EVDNERNWSDKIPAA LPSSSEHSKS
Sbjct: 432 MQRISSLAIFCDRPDWLDLWAEMELSDALAKLQMEVDNERNWSDKIPAATLPSSSEHSKS 491

Query: 361 PAISTVFIKHLSSLVYRCQSLPSITLRSRFFKLAGSPIIANVFNCVLIRCQEAEGLTALT 420
           PAIS+VFIKHLSSLVYRC+SLP+I+LRSRFFKLAGSPIIA    CVLIRCQEAEGLTALT
Sbjct: 492 PAISSVFIKHLSSLVYRCRSLPTISLRSRFFKLAGSPIIAKFLRCVLIRCQEAEGLTALT 551

Query: 421 DDDALVKVANSINAARYFESILKEWCEDMFFLEMGTASDE---------------LLASP 480
           DDDAL+KVANSINAARYFESILKEW EDMFFLEMG+ SD                LL S 
Sbjct: 552 DDDALIKVANSINAARYFESILKEWREDMFFLEMGSVSDGQLGVLDGDGDGDVDGLLESR 611

Query: 481 STGIIDSEIRKFEEFRREWVEKISTVILRGFDAQSRDYIKNKKQWKEKFEDGWTVSRLLI 540
           STGIID EIR  EEFR+EWVEKISTVILRGFDA+SRDYIKNKKQWKEK EDGWTVSRLL+
Sbjct: 612 STGIIDQEIRNLEEFRQEWVEKISTVILRGFDAESRDYIKNKKQWKEKSEDGWTVSRLLV 671

Query: 541 GALDYLQGKMLTLEKNLNGIDFVSLWRTLAAGVDRFIFNGILMSNVQFNNDGVKRFGDDM 600
           GALDYL GKM TLE+NLNGIDFV LWRTLAAGVDRF+FNGI+MSNVQFN+ GVKRFGDDM
Sbjct: 672 GALDYLAGKMSTLEENLNGIDFVFLWRTLAAGVDRFLFNGIMMSNVQFNDVGVKRFGDDM 731

Query: 601 EVLFGIFRSWCLRPEGFFPKISESMKLLKMKEEQLKSSLVGEQSWMKENGVKHLSTSEVD 649
           EVLFGIFRSWCLRPEGFFPK+SESM+LLKMKEEQLKSSLVG Q+WMKENG+KHLST E D
Sbjct: 732 EVLFGIFRSWCLRPEGFFPKLSESMRLLKMKEEQLKSSLVGGQAWMKENGLKHLSTIEAD 791

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STU32.3e-23159.55RINT1-like protein MAG2 OS=Arabidopsis thaliana OX=3702 GN=MAG2 PE=1 SV=1[more]
Q8GXP15.4e-10835.04RINT1-like protein MAG2L OS=Arabidopsis thaliana OX=3702 GN=MAG2L PE=2 SV=1[more]
Q8BZ361.1e-2021.94RAD50-interacting protein 1 OS=Mus musculus OX=10090 GN=Rint1 PE=1 SV=2[more]
Q6NUQ19.5e-2021.32RAD50-interacting protein 1 OS=Homo sapiens OX=9606 GN=RINT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_011658877.10.099.23RINT1-like protein MAG2 isoform X2 [Cucumis sativus][more]
XP_004137086.10.099.23RINT1-like protein MAG2 isoform X1 [Cucumis sativus] >KGN43850.1 hypothetical pr... [more]
XP_008455035.10.096.76PREDICTED: RINT1-like protein MAG2 isoform X2 [Cucumis melo][more]
XP_008455034.10.096.76PREDICTED: RINT1-like protein MAG2 isoform X1 [Cucumis melo] >KAA0031366.1 RINT1... [more]
XP_038888330.10.091.27RINT1-like protein MAG2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0K2F10.099.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071390 PE=4 SV=1[more]
A0A5D3C6N80.096.76RINT1-like protein MAG2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C0P80.096.76RINT1-like protein MAG2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495307 PE=4 ... [more]
A0A1S3BZY30.096.76RINT1-like protein MAG2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495307 PE=4 ... [more]
A0A6J1I9Z00.087.13RINT1-like protein MAG2 OS=Cucurbita maxima OX=3661 GN=LOC111471001 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007528RINT-1/Tip20PFAMPF04437RINT1_TIP1coord: 160..647
e-value: 3.2E-33
score: 115.3
IPR007528RINT-1/Tip20PANTHERPTHR13520RAD50-INTERACTING PROTEIN 1 RINT-1coord: 1..648
IPR007528RINT-1/Tip20PROSITEPS51386RINT1_TIP20coord: 69..649
score: 117.907127
IPR042044EXOC6/PINT-1/Sec15/Tip20, C-terminal, domain 2GENE3D1.20.58.670Dsl1p vesicle tethering complex, Tip20p subunit, domain Dcoord: 465..589
e-value: 2.9E-8
score: 35.6
NoneNo IPR availablePANTHERPTHR13520:SF1RINT1-LIKE PROTEIN MAG2coord: 1..648

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G9278Cucsat.G9278gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G9278.T4.E1Cucsat.G9278.T4.E1exon
Cucsat.G9278.T4.E2Cucsat.G9278.T4.E2exon
Cucsat.G9278.T4.E3Cucsat.G9278.T4.E3exon
Cucsat.G9278.T4.E4Cucsat.G9278.T4.E4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G9278.T4.C2Cucsat.G9278.T4.C2CDS
Cucsat.G9278.T4.C1Cucsat.G9278.T4.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G9278.T4Cucsat.G9278.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060628 regulation of ER to Golgi vesicle-mediated transport
biological_process GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
cellular_component GO:0070939 Dsl1/NZR complex