Cucsat.G9130.T38 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G9130.T38
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Lipase
Location	ctg1658: 191783 .. 197149 (+)
RNA-Seq Expression	Cucsat.G9130.T38
Synteny	Cucsat.G9130.T38

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGGTGAAGAAATGGGTTTTTTGTATTAAAATGAAGCCATTCTCAAGCAACGAATATGCCACGCGCGCCTTATTAGCGGGCGCGGGCGTGGACACAACCACCAACCGATTCGACTAGTTGAATGGCATCTTCTCTTAGTTTCCTCTTTCCCCAAATAATATATATATATATACAGTGAACAGTTTTTATTATCAATTTGTTAACTAATTCTCCTCGCCCTGACTTCAAGGTTAATCTTTGTTACGGATATGGCGATGCCATTGCTTGCTTTCATATTTCTTCTCTTCTTCTTCCCTCTCCATTCCGCCGGTGATTCCTCCTTCCCTTCTGTCGTTGACTACTCGATTCTCCCTCACGCTTTGTCCGACAACAAGTCCTTGTGCTCCCAACTCGTTCTTCCTGCTGGCTATCCTTGTGCTGAGCATAAGGTTCTCTATTTCTCTCATTTCGCTTCCAATTTTTTGGTTGATTAATTAGAGGAAGGTGTGGACAATTATGTGAAATGAGACTGTAATATTAGAGTTCATCGCTCTGTTTCGACTGGAGATACTTGGATGGATTTTAGTCAACTAATTGCCTCTAATGCTTCTTGTTTGAGATTTCCTTTGAGTTTCTTGTTTATTTTCTTTTCTGAGGTGAGACAGATGGAATTAATTTCTTTGTATTTTTAGATTCGATGCCCGACGTGGTTTTGATTTGTTTCTGTCGATTTTCTGGATAATCCGATGTATATGTATCGTTCTCGAAAGTGTGATATGCGAAGAAATATGATTGAATTTGATTTTTATGTAGATCTCTGATTTTATCAATTTCTCATTAACCATTTGGAGCTTAATGCATCTCTACTGTGTAAATTTGTTACTATAAATGATGTTGTTCTTTGATCATGCTCGAGTTTTGAGGAATGTTATCCATTATTGCAGACTGGGCTGATGAATTGTGCTTTTAGTAATGCACTTGAGTACGGTTTTGCAGTACTGAGTCACTGACGGACGTGATTTTCTCATAAATTTCTTAGATTCAAACAAAGGATGGGTTTTTATTGGGTCTGCAACGTGTATCTTCACGTGATGGAGAATTGGAAAAGCAAAAAGGTCCTCCAATTTTGCTTCTCCATGGATTGTTTATGGTAAGTGTTCTGACAGTTTTGCAATATTGTAACTGTATCTGTTAGGTCTTTTTACTCTTTTTTTGGTCTAACTCTATTAGCAATAGCTAGGGTATTGACATTGCTGCCTGATATCTTTTATACTACCGAGACTTATCTCCTTTTGTTGTAATTTTCAACTGCTCCCATCTCTTCATATTCCAGTGGTATTTAATGTTCTCGATTAGGTCGATATTTACCTTAAACTTCTACACTACAGTCCAATAGTGTAACCATGCTTTCCAGTTATCTCTCAAGGAATTGGTACTAGAAGATGAATGGCATATAGCCTCAAGGCAGATTTTACCCAAGGCCCTATCTTTTATATATATATGTCTCTCTCTTTTTAATCTTCTGGCCTCTGTTTTCTTGTCTCTCCAAGTGAGATTCTTTTCAAATTCTTTTTATTCTCATCCCAAAGTGTACTGAATTGTCTTCTTTAGTTTCATTCGTTGGTACAACATTTCTATGCTTGGGAAGATCCAATTATCCATAAATTTCTTTTTGTCTAGGAGGTTAGTAACTGCGTATTTTGAAACATGGATGGTGAATTTTGAATAAGTTTGTAGTCTGGGATCATTCTCACAGTAAGGGGTTCCAACCACTAGTTTTTCGTACCTTTCATTGTCTGAGCTGGTGCATGTGCAGGCTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTCCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTTATGGGCATTCATCCCTATCAGAGGATGAGAAGGTATTCTTAGTGTACTTTATCACAAAATTTTAAGATGTGTAAGATGTTAATTAAATGCAGAGAAAGAACATAGTTTCCCCTATTGTGTTGTGGATGTATTGCATGTATCTAGGAAGTTAAATAATGATACAAACATGGTGTAGCTCATGCAGGCAATTTTGACGCTTCAAACTTCAAACAAATTTACATGACGTTTTATGGAGTTAAGCAGTCGCATGACAGATTATCTTCAAGGATCATTTGATTTCATGCTAATTGTGTTGATTTGCTAAAGTTTATTTTATCTCAGAATTCTGCACTTTATTGTATGGTGGATTCTTGGTCCACAAGTATGATAGGACAATCTTGAAAGTACTCTAACATAAATTTCATTTGTTTAGTTCTATTTATGTCTTTGACTCTAGTATATTATGATTGTAGGAATTTTGGAATTGGAGTTGGGAAGAGTTGGCCCTGTACGATCTTGCAGAAATGATTAACTACATAAATTCCTTGACAAACAAGAAAATTTATATTGTGGGACACTCACAGGTCTCATATCTCTTCTTCTACGAGATTGTTGCTCTGAAATGATATATTTCTGGTATGGTGTCATCTTTTATCTTTGTTCGCAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGGAGCAGCTGCTCTTTTAAGTCCTATATCGTACTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGGTATTCTTGGCTTGTTATTGTGCAATTAGCATTGTTAAGTGATTGTATTATTAATAGCAATAAGCTTATTTTTTATATTTTTTTCGACAGATAATTCTTGCCTCAGGATTTCATGAACTCAACTTTAAAAGGTTGTCATGCTATGCTGTGCCCATCTCTAACTTTGTAATGGATTGCATTTTTAGCTTCACCATAGTTCATGGTACTCATGCCTATGTTTTTGTATGCTATTTCAGTGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTAGTTAACTGCATCAATATTCTAAGTTCAATAACAGGTTCTCTCAATGCAACCTGCTTCACGTTTCTCTCTTTTCTTTTTATTTATTATTTTTGTGAAACCTTGACTTTGGAATCCTTATCCAACATACTTGGATGATCCTTTCTTTAACCGAATACGAATCTTTCGATTCATTTGGATCTCCTCTCACGCTTATTTATTGCTTTTTACTTTTGGGAAATAATTGAACACTAATCCAAGACTCTAATATTCAGAGGGCTCTATAATTGTCAGCAAAGTTGTTTTCCTTTTCTTGAAATCCAAAAACAAAAATTCTTACTTTATACATAACATAGGTCATCCATTCAACATGGGATAAAAAAGAAACAAATGAGGGTGAAATCTTGCTAATTTCTTTTAATTACTATATCTTATACTGACATCAATCATATTGGTTCATAATTTTGTGTGGTTAGTGGGTATCTCTCTTAAAACTGAAGGGCTTGGGTTGGAAAATATGACTAAAATTTACCTCGTGATGGAATTGGGAAAAATATTACTGATATTTACCAAGTTCCCCATTTTTTACATGATATTGCTGCCAGCAAGCTTCTATTAGACATATTGCAATTCAAGAAATGGACTACCAGCTTCCATTTTTTGCTCTGATGCATATCTGCATTTTCATCCATTTTATTGAACCTTAACTTCATTTGCTATTATGAAGTTTGGATGTTCAACACTAAATTTCCTAAGGATGCCTTCTTTTAAATGCTGCAGGCGAAAATTGCTGCCTGAATCGCTCACGTTTTGACTTATTTTTCAAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGAGTATGCTCTGCAATATCTTCTCTCTACTTCAACTTCTATCATACTATGACTATGATTTCGATGAATCTTTGAATGTTTTACCTGCATGTATTTCTTGGAAAGTTAATCATAGTGTTCTCTTGTGAAGTCTAATATAAATCTTTAAACATTTGATCGTGAAGCTTTGCATCATACATTCTAAACTCTTTCAAGACTTAGAAGATAATTTGATCTTCCTCACATGACATTTTTCTAGAAACCTTTTAAAGATATCAAAATTAACCCAACCTCAACCTAAAGGATACTCAGCTTTCCACAAACAGATTTTCATTCAGACACCAAATTTGCTAATACTCTGCAACCTTAAGCAGTTTTTCTTCCTTTGGCTTCCTTCTTTTAATTTGCTTCCCACTTTCCCGATTTTTCAAATAATCGAGGAGGCTATAAAGGATTTTTTTTTCATATGCTCATTAACTTCTTTCGCTCAATCTCTTTCCTGAGTGACTTTTCATCATCCCTCAAATCCCTCGCTTTCCATTAACTTTATCTATAATTAAAGCCAGCCCTCTATTTCCATAGTATTTTTATCAATTACCGAACTCGTTTCCTCTTTTCACTCCCTTGTCCTTACCTTCGCTTTCCACCTATTCCTTTCAATCAAAGCTTCAATTTCCGCTTGCGCCACCAGAACTTTTCTGTTCTCTTTAACATTAGGCACAAACAACATAATCGAACTTTGCTTATTATTACCAAGCCGCTTGCTCTTTAGCAGTTCATTTTTCTCCATCTTGTACTTCCAAGTCCGAACATTAGTGATGGTTTAGGATCAATTTTTCTGTTGTTAGTTCTATTAATTTTATTAGGAGAGTGTATACTCGACTTCAACACATGGCAACAATTTACTACACTGAAGTTGAATTATTGGTTTTAGGAAGTGTTATAGTCTACAAATCAGTTGCTTGGTTAAGACTTAAGCAGTATGTTTTCTCCTCCTGACGGGGATACGACGTGGTTCGACTCCACGGACTTAACTTCCTTTTCCTTTTCTTCCACCAGTGATCCGCAAAGGTAGTTTTTCCAAGTATGACTATGGATTGTTAAAGAATCTGAGAGTTTATGGTCAAAGGGTGCCACCTGAATTTGATCTCAGCCGCATTCCTGAATCCTTGCCGCTGTGGATGGCATACGGCGGGAACGATGAGTTGTCAGATTGGACAGATCTGGAGAACACTATAAAGAAGGTGAAGTCGGTACCAGAATTAGTTTACCTTGAGAATTATGGTCATGTGGATTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAGTTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAAAGTGAATTGTGGTTAATGTGGTTGTAAATGTGTGCTGGTGTTGATATAAATGTGTGTTGTGGTAGTAGCCAAATCCTTCGTTGTTATGGAGGGCTAATTATTGTTCTATTTGTTTGATATTGAATTCCTTTAACTTAATAAAAATACCAAATAAATGAAATAATAAAAGAAGAATTATTTGTA

Coding sequence (CDS)

ATGGCGATGCCATTGCTTGCTTTCATATTTCTTCTCTTCTTCTTCCCTCTCCATTCCGCCGGTGATTCCTCCTTCCCTTCTGTCGTTGACTACTCGATTCTCCCTCACGCTTTGTCCGACAACAAGTCCTTGTGCTCCCAACTCGTTCTTCCTGCTGGCTATCCTTGTGCTGAGCATAAGATTCAAACAAAGGATGGGTTTTTATTGGGTCTGCAACGTGTATCTTCACGTGATGGAGAATTGGAAAAGCAAAAAGGTCCTCCAATTTTGCTTCTCCATGGATTGTTTATGGCTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTCCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTTATGGGCATTCATCCCTATCAGAGGATGAGAAGGAATTTTGGAATTGGAGTTGGGAAGAGTTGGCCCTGTACGATCTTGCAGAAATGATTAACTACATAAATTCCTTGACAAACAAGAAAATTTATATTGTGGGACACTCACAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGGAGCAGCTGCTCTTTTAAGTCCTATATCGTACTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGATAATTCTTGCCTCAGGATTTCATGAACTCAACTTTAAAAGGTTGTCATGCTATGCTGTGCCCATCTCTAACTTTGTAATGGATTGCATTTTTAGCTTCACCATAGTTCATGGCGAAAATTGCTGCCTGAATCGCTCACGTTTTGACTTATTTTTCAAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGATGATCCGCAAAGGTAGTTTTTCCAAGTATGACTATGGATTGTTAAAGAATCTGAGAGTTTATGGTCAAAGGGTGCCACCTGAATTTGATCTCAGCCGCATTCCTGAATCCTTGCCGCTGTGGATGGCATACGGCGGGAACGATGAGTTGTCAGATTGGACAGATCTGGAGAACACTATAAAGAAGGTGAAGTCGGTACCAGAATTAGTTTACCTTGAGAATTATGGTCATGTGGATTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAGTTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Protein sequence

MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL

Homology

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BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 6.8e-133
Identity = 228/380 (60.00%), Postives = 285/380 (75.00%), Query Frame = 0


Query: 27  SVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEKQKG 86
           S +  S L H  S   SLC+ L+ PA Y C EH IQTKDG++L LQRV+S    L  Q G
Sbjct: 16  SALTQSHLLHG-SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSG 75

Query: 87  PPILLLHGLFMAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEF 146
           PP+LL HGLFMAGD WFLNS ++SLGFIL D+GFDVW+GNVRGTR+SYGH +LS+ +KEF
Sbjct: 76  PPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEF 135

Query: 147 WNWSWEELALYDLAEMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLS 206
           W+WSW++LA+YDLAEMI Y+ S++N KI++VGHSQGTIMSFAALTQP +A+ V AAALL 
Sbjct: 136 WDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLC 195

Query: 207 PISYLEHITAPLVRLMVDTHLDTIILASGFHELNFK--RLSCYAVPISNFVMDCIFSFTI 266
           PISYL+H+TAPLV  MV  HLD +++A G H++NF+   L      +    MDC    T 
Sbjct: 196 PISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTS 255

Query: 267 VHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVP 326
           + G NCC N S+ + +  YEPHPSS KN+ HLFQMIRKG+F++YDYG  KNLR YG   P
Sbjct: 256 ITGTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKP 315

Query: 327 PEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKA 386
           PEF LS IP SLP+WM YGG D L+D TD+E+T+ ++ S PEL+YLE+YGH+DF+L   A
Sbjct: 316 PEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSA 375

Query: 387 KEDVYDPMIKFFKSLGKSSS 405
           KEDVY  MI+FF++  KSSS
Sbjct: 376 KEDVYKHMIQFFRAKVKSSS 392

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BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.9e-75
Identity = 143/370 (38.65%), Postives = 218/370 (58.92%), Query Frame = 0


Query: 44  LCSQLVLPAGYPCAEHKIQTKDGFLLGLQRV-SSRDGEL--EKQKGPPILLLHGLFMAGD 103
           +C+  V   GY C EH + T+DG++L +QR+   R G +  +  K  P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 104 GWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLA 163
            W LN A Q+L  IL D GFDVW+GN RGTR+S  H  L+  ++ FWNW+W+EL  YDL 
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 164 EMINYINSLTNKKIYIVGHSQGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVR 223
            M ++I+ LT +KI+ +GHS GT++ FA+ ++  +  +V +AA+LSP++YL H+T  +  
Sbjct: 171 AMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGD 230

Query: 224 LMVDTHLDTIILASGFHELN--------FKRLSCYAVPISNFVMDCIFSFTIVHGENCCL 283
           +   T L       G+ E N        F +  C    I     DC    +++ G+NCCL
Sbjct: 231 IAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGI-----DCYDLVSVITGKNCCL 290

Query: 284 NRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLL-KNLRVYGQRVPPEFDLSR 343
           N S  DLF   EP  +S KN+ HL Q +R     KY+YG   +N++ YGQ +PP +++S 
Sbjct: 291 NASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISA 350

Query: 344 IPESLPLWMAYGGNDELSDWTDLENTIKKVK----SVPELVYLENYGHVDFILSMKAKED 398
           IP  LPL+ +YGG D L+D  D+E  + + K        + ++++Y H DFI+ + AK+ 
Sbjct: 351 IPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDV 410

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BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.6e-52
Identity = 122/360 (33.89%), Postives = 197/360 (54.72%), Query Frame = 0


Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRVSSRDGELEK-QKGPPILLLHGLFMAGDGWFL 105
           SQ++   GYPC E+++ T+DG++LG+ R+       E   K P + L HGL  +   W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N    SL F+L D G+DVW+GN RG  WS  +   S D  EFW +S++E+A YDL   IN
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  +KI+ VGHSQGT + F A  T P +AKK+     L+P++ +++  +PL ++ 
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKI- 214

Query: 226 VDTHLDTIILASGFHELNFKRLSCY-----AVPISNFVMDCIFS---FTIVHGENCCLNR 285
             + + T +    F +  F   + +         S  V+D + S   F     +   LN 
Sbjct: 215 --SFIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNV 274

Query: 286 SRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGL-LKNLRVYGQRVPPEFDLSRIP 345
           SRFD++  + P  +S ++  H  Q++R G F  +++G   +N+  Y Q+ PPE+D+S + 
Sbjct: 275 SRFDVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMT 334

Query: 346 ESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 394
             + +W   GGND L+D  D+   + K+ ++     +  Y H+DFI +M A ++VY+ MI
Sbjct: 335 VPVAVW--NGGNDILADPQDVAMLLPKLSNLLFHKEILAYNHLDFIWAMDAPQEVYNEMI 389

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BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot
Match: Q5VXJ0 (Lipase member K OS=Homo sapiens OX=9606 GN=LIPK PE=2 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-49
Identity = 116/360 (32.22%), Postives = 191/360 (53.06%), Query Frame = 0


Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRV-SSRDGELEKQKGPPILLLHGLFMAGDGWFL 105
           SQ++   GYP  E+ + TKDG++LG+ R+   R         P + L HGL  +   W  
Sbjct: 36  SQIISYWGYPYEEYDVTTKDGYILGIYRIPHGRGCPGRTAPKPAVYLQHGLIASASNWIC 95

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N    SL F+L D+G+DVW+GN RG  WS  H  LS    E+W +S +E+A YDL   IN
Sbjct: 96  NLPNNSLAFLLADSGYDVWLGNSRGNTWSRKHLKLSPKSPEYWAFSLDEMAKYDLPATIN 155

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  K++Y VGHSQGT ++F A  T P++AKK+     L+P+  +++  +P+ +L 
Sbjct: 156 FIIEKTGQKRLYYVGHSQGTTIAFIAFSTNPELAKKIKIFFALAPVVTVKYTQSPMKKL- 215

Query: 226 VDTHLDTIILASGFHELNFKRLSCY----AVPISN---FVMDCI-FSFTIVHGENCCLNR 285
             T L   ++   F +  F   + +    A  + N   F   C  F FT+   +   LN 
Sbjct: 216 --TTLSRRVVKVLFGDKMFHPHTLFDQFIATKVCNRKLFRRICSNFLFTLSGFDPQNLNM 275

Query: 286 SRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLL-KNLRVYGQRVPPEFDLSRIP 345
           SR D++  + P  +S +N+ H  Q +  G    +D+G   +N+  + Q  PP ++++++ 
Sbjct: 276 SRLDVYLSHNPAGTSVQNMLHWAQAVNSGQLQAFDWGNSDQNMMHFHQLTPPLYNITKME 335

Query: 346 ESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 394
               +W   GG D ++D  D+EN + ++ ++     + +Y HVDF L   A +++Y  +I
Sbjct: 336 VPTAIW--NGGQDIVADPKDVENLLPQIANLIYYKLIPHYNHVDFYLGEDAPQEIYQDLI 390

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BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot
Match: Q29458 (Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.6e-49
Identity = 114/359 (31.75%), Postives = 189/359 (52.65%), Query Frame = 0


Query: 46  SQLVLPAGYPCAEHKIQTKDGFLLGLQRV-SSRDGELEKQKGPPILLLHGLFMAGDGWFL 105
           SQ++   GYP   HK+ T DG++L + R+   ++      + P + L HGL  +   W  
Sbjct: 35  SQMISYWGYPSEMHKVITADGYILQVYRIPHGKNNANHLGQRPVVFLQHGLLGSATNWIS 94

Query: 106 NSARQSLGFILPDNGFDVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMIN 165
           N  + SLGF+L D G+DVW+GN RG  W+  H   S D  EFW +S++E+A YDL   I+
Sbjct: 95  NLPKNSLGFLLADAGYDVWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTID 154

Query: 166 YINSLT-NKKIYIVGHSQGTIMSFAAL-TQPDIAKKVGAAALLSPISYLEHITAPLVRLM 225
           +I   T  KK++ VGHSQGT + F A  T P +A+K+     L+P++ +++  +   +L 
Sbjct: 155 FILRRTGQKKLHYVGHSQGTTIGFIAFSTSPTLAEKIKVFYALAPVATVKYTKSLFNKLA 214

Query: 226 VDTHL-------DTIILASGFHELNFKRLSCYAVPISNFVMDCIFSFTIVHGENCCLNRS 285
           +  H        D +     F E       C    +     + +F+ T V  +N   N S
Sbjct: 215 LIPHFLFKIIFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKN--FNMS 274

Query: 286 RFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGL-LKNLRVYGQRVPPEFDLSRIPE 345
           R D++  + P  +S +N  H  Q ++ G F  +D+G   +NL  Y Q  PP ++L+ +  
Sbjct: 275 RLDVYIAHNPAGTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNV 334

Query: 346 SLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 394
            + +W A   ND L+D  D++  + K+ ++     + NY H+DFI +M A ++VY+ ++
Sbjct: 335 PIAVWSA--DNDLLADPQDVDFLLSKLSNLIYHKEIPNYNHLDFIWAMDAPQEVYNEIV 389

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BLAST of Cucsat.G9130.T38 vs. NCBI nr
Match: KAE8645852.1 (hypothetical protein Csa_017220 [Cucumis sativus])

HSP 1 Score: 808 bits (2088), Expect = 1.08e-294
Identity = 405/442 (91.63%), Postives = 405/442 (91.63%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNFVMDCIFSFTIVHG--------------------------------- 300
           FKRLSCYAVPISNFVMDCIFSFTIVHG                                 
Sbjct: 241 FKRLSCYAVPISNFVMDCIFSFTIVHGTHAYVFVCYFSDWGTVLLDNLCDRLVNCINILS 300

Query: 301 ----ENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360
               ENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV
Sbjct: 301 SITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRV 360

Query: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 405
           PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK
Sbjct: 361 PPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMK 420

[Close]

BLAST of Cucsat.G9130.T38 vs. NCBI nr
Match: XP_004136959.1 (triacylglycerol lipase 1 [Cucumis sativus])

HSP 1 Score: 773 bits (1995), Expect = 4.30e-281
Identity = 385/408 (94.36%), Postives = 391/408 (95.83%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFFKYEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT
Sbjct: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 407

[Close]

BLAST of Cucsat.G9130.T38 vs. NCBI nr
Match: XP_008454984.1 (PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa] >TYK06787.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 739 bits (1909), Expect = 5.48e-268
Identity = 365/408 (89.46%), Postives = 385/408 (94.36%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFF+YEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKGSFSKYDYGLLKNLRVYGQR+PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+T
Sbjct: 301 QMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 407

[Close]

BLAST of Cucsat.G9130.T38 vs. NCBI nr
Match: XP_038886839.1 (triacylglycerol lipase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 691 bits (1783), Expect = 8.28e-249
Identity = 341/408 (83.58%), Postives = 373/408 (91.42%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL++ IFLLFFFPLHSA +SSF SV +YSILPHA SD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MATPLMSLIFLLFFFPLHSAHNSSFSSV-EYSILPHARSDQKSLCSQLVHPAGYPCAEHT 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDG+LEK+KGPPILLLHGLFM GDGWFLNSA+QSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGDLEKRKGPPILLLHGLFMGGDGWFLNSAKQSLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSED+KEFWNWSWEELALYDLA MINYINSLT++KIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDDKEFWNWSWEELALYDLAAMINYINSLTDRKIYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKV AAALLSPISYLEHITAPLVRLMVDTHLDTIIL++GF ELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILSAGFRELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFF+YEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKG+FS+YDYGLLKN+RVYGQR PPEFDLSRIP+SLPLWMAYGGNDELSDWTDLE+T
Sbjct: 301 QMIRKGTFSRYDYGLLKNMRVYGQRKPPEFDLSRIPKSLPLWMAYGGNDELSDWTDLEHT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IK++K VPELVYLENYGHVDFILSM AK+D+YDPMIKFFKSL KSSS+
Sbjct: 361 IKELKPVPELVYLENYGHVDFILSMNAKKDLYDPMIKFFKSLAKSSSM 406

[Close]

BLAST of Cucsat.G9130.T38 vs. NCBI nr
Match: XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677 bits (1748), Expect = 1.77e-243
Identity = 334/404 (82.67%), Postives = 367/404 (90.84%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S+ +YSILP ALSD KSLCSQLV P GYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSL-EYSILPRALSDQKSLCSQLVQPVGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+S+SEDEKEFW+WSWE+LALYDLA MI+YINSLT +KIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRL--SCYAVPISNFVMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQ 300
           FK    +     + + +++CI   + + GENCCLN SRFDLFF+YEPHPSSAKNLHHLFQ
Sbjct: 241 FKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQ 300

Query: 301 MIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTI 360
           MIRKG+FS+YDYGLLKNLRVYGQR PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TI
Sbjct: 301 MIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTI 360

Query: 361 KKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKS 402
           K++KSVPELVYLENYGHVDFILSMKAKEDVYDPMIKF KSLGKS
Sbjct: 361 KELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

[Close]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL
Match: A0A0A0K4L2 (Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1)

HSP 1 Score: 773 bits (1995), Expect = 2.08e-281
Identity = 385/408 (94.36%), Postives = 391/408 (95.83%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFFKYEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT
Sbjct: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 407

[Close]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL
Match: A0A5A7SJL6 (Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 2.65e-268
Identity = 365/408 (89.46%), Postives = 385/408 (94.36%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFF+YEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKGSFSKYDYGLLKNLRVYGQR+PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+T
Sbjct: 301 QMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 407

[Close]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL
Match: A0A1S3C115 (Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 2.65e-268
Identity = 365/408 (89.46%), Postives = 385/408 (94.36%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FL++FFPLHSAGDSS P+VVDYSILPHALSD+KSLCSQLVLPAGYPCAEHK
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSSR+G+LEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTN+KIYIVGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQP+IAK V AAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKRLSCYAVPISNF---VMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLF 300
           FK      V + N    +++CI   + + GENCCLNRSRFDLFF+YEPHPSSAKNLHHLF
Sbjct: 241 FKS-DWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLF 300

Query: 301 QMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENT 360
           QMIRKGSFSKYDYGLLKNLRVYGQR+PPEFDLS IPESLPLWMAYGGNDELSDWTDLE+T
Sbjct: 301 QMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHT 360

Query: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 IKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 407

[Close]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 9.47e-243
Identity = 333/407 (81.82%), Postives = 368/407 (90.42%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S+ +YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSL-EYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSEDEKEFW+WSWEELALYDLA MI+YINSLT +K+Y+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIA+KV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRL--SCYAVPISNFVMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQ 300
           FK    +     + + +++CI   + + GENCCLN SRFDLFF+YEPHPSSAKNLHHLFQ
Sbjct: 241 FKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQ 300

Query: 301 MIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTI 360
           MIRKG+FS+YDYGLLKNLRVYGQR PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TI
Sbjct: 301 MIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTI 360

Query: 361 KKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKSSSL 405
           K++KSVPELVYLENYGHVDFILSMKAKEDVYDPMIKF KSLGKS +L
Sbjct: 361 KELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406

[Close]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL
Match: A0A6J1GMQ3 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 673 bits (1737), Expect = 4.06e-242
Identity = 332/404 (82.18%), Postives = 366/404 (90.59%), Query Frame = 0


Query: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60
           MA PL+A +FLL FF LHSA DSS+ S+ +YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSL-EYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60

Query: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120
           IQTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120

Query: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180
           DVWIGNVRGTRWS+GH+SLSEDEKEFW+WSWEELALYDLA MI+YINSLT +K+Y+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHS 180

Query: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240
           QGTIMSFAALTQPDIA+KV AAALLSPISYLEHITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELN 240

Query: 241 FKRL--SCYAVPISNFVMDCIFSFTIVHGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQ 300
           FK    +     + + +++CI   + + GENCCLN SRFDLFF+YEPHPSSAKNLHHLFQ
Sbjct: 241 FKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQ 300

Query: 301 MIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTI 360
           MIRKG+FS+YDYGLLKNLRVYGQR PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TI
Sbjct: 301 MIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTI 360

Query: 361 KKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFFKSLGKS 402
           K++KSVPELVYLENYGHVDFILSMKAKEDVYDPMIKF KSLGKS
Sbjct: 361 KELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

The following BLAST results are available for this feature:

BLAST of Cucsat.G9130.T38 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q71DJ5	6.8e-133	60.00	Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1	[more]
Q67ZU1	4.9e-75	38.65	Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1	[more]
P04634	2.6e-52	33.89	Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1	[more]
Q5VXJ0	2.1e-49	32.22	Lipase member K OS=Homo sapiens OX=9606 GN=LIPK PE=2 SV=2	[more]
Q29458	3.6e-49	31.75	Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1	[more]

BLAST of Cucsat.G9130.T38 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8645852.1	1.08e-294	91.63	hypothetical protein Csa_017220 [Cucumis sativus]	[more]
XP_004136959.1	4.30e-281	94.36	triacylglycerol lipase 1 [Cucumis sativus]	[more]
XP_008454984.1	5.48e-268	89.46	PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 tria...	[more]
XP_038886839.1	8.28e-249	83.58	triacylglycerol lipase 1 isoform X1 [Benincasa hispida]	[more]
XP_023553816.1	1.77e-243	82.67	triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G9130.T38 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4L2	2.08e-281	94.36	Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1	[more]
A0A5A7SJL6	2.65e-268	89.46	Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=...	[more]
A0A1S3C115	2.65e-268	89.46	Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1	[more]
A0A6J1GLC0	9.47e-243	81.82	Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1	[more]
A0A6J1GMQ3	4.06e-242	82.18	Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006693	Partial AB-hydrolase lipase domain	PFAM	PF04083	Abhydro_lipase	coord: 47..105 e-value: 1.8E-16 score: 59.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 35..398 e-value: 3.0E-109 score: 367.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 46..397
IPR025483	Lipase, eukaryotic	PIRSF	PIRSF000862	Steryl_ester_lip	coord: 1..401 e-value: 1.5E-120 score: 400.7
None	No IPR available	PANTHER	PTHR11005	LYSOSOMAL ACID LIPASE-RELATED	coord: 36..399
None	No IPR available	PANTHER	PTHR11005:SF118	TRIACYLGLYCEROL LIPASE 1	coord: 36..399

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G9130	Cucsat.G9130	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9130.T38.E1	Cucsat.G9130.T38.E1	exon
Cucsat.G9130.T38.E2	Cucsat.G9130.T38.E2	exon
Cucsat.G9130.T38.E3	Cucsat.G9130.T38.E3	exon
Cucsat.G9130.T38.E4	Cucsat.G9130.T38.E4	exon
Cucsat.G9130.T38.E5	Cucsat.G9130.T38.E5	exon
Cucsat.G9130.T38.E6	Cucsat.G9130.T38.E6	exon
Cucsat.G9130.T38.E7	Cucsat.G9130.T38.E7	exon
Cucsat.G9130.T38.E8	Cucsat.G9130.T38.E8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9130.T38.C1	Cucsat.G9130.T38.C1	CDS
Cucsat.G9130.T38.C2	Cucsat.G9130.T38.C2	CDS
Cucsat.G9130.T38.C3	Cucsat.G9130.T38.C3	CDS
Cucsat.G9130.T38.C4	Cucsat.G9130.T38.C4	CDS
Cucsat.G9130.T38.C5	Cucsat.G9130.T38.C5	CDS
Cucsat.G9130.T38.C6	Cucsat.G9130.T38.C6	CDS
Cucsat.G9130.T38.C7	Cucsat.G9130.T38.C7	CDS
Cucsat.G9130.T38.C8	Cucsat.G9130.T38.C8	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9130.T38	Cucsat.G9130.T38-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0048580	regulation of post-embryonic development
biological_process	GO:2000033	regulation of seed dormancy process
biological_process	GO:0010029	regulation of seed germination
biological_process	GO:0009739	response to gibberellin
cellular_component	GO:0016020	membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0004806	triglyceride lipase activity