Cucsat.G9024.T27 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G9024.T27
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	NAD(P)-linked oxidoreductase superfamily protein
Location	ctg1635: 1110139 .. 1113301 (+)
RNA-Seq Expression	Cucsat.G9024.T27
Synteny	Cucsat.G9024.T27

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGCTCGAAATCCTTCTAATAAAAAACAATTCCAAGTTTTTCTTTAGATTCATTAAAGAAAGAAAGCGAAAGGATCCTGGATTTGAGATCACAATTGCTACCAAATATGCGGTTTTTCCATGGAGGCTCGGTCGTCACAGTGTCGTAAGTGTTCTCAAAGAATCACTTTGTCGTCTCGGTCTCGAATCGGTTGACTTGTATCAACTTCATTGGTATGCGCTGCTCTTTAAACTCTCTTCTCATGTTCTTGTTTTACTGTAGTTATTTACTCATCGATCCCTTTGAATTTCAGGCCTGGAATATGGGGAAATGAAGGTTTGTTCAAAGTAGTTTGAAAGTTATTTGAATTTTTTTTTTCTTTTGAAGGATTTAATGACCATATGATTGTGAAGAAGAAGCCTAATTAAGTTCATAAAGAAATCATTTTTCAGAGTATATTGATGGTCTTGGAGATGCTGTTGAGCAAGGACTTGTGAAGGGTGTTGGTGTTTCTAATTATAATGGTATATGTTCTAATCTTAATTTTGGTATATATACAAACTTAATTAATAATGTGATGGCAAACCTTTTATAATTTTTGTTGTCCTATAAAAATGAAATATATTTGTTAATAAAAGTGTGTAGTTCTGGTCGTATATGTATCCTATTTTCTTCTATAAATTTCCACTGCATGCATGCATGCATGTCATGTTGTGTTTATGTCTTATATTCATTTTCTATAATGTAGAGAAGCGACTTCGAGATGCGTATGAAAAACTAAAGAAAAGAGGTATTCCACTAGCTTCAAATCAAGTGAATTATAGCCTCATATATAGATTACCTGAAGAGAACGGTGTGAAGGCTGCTTGTGATGAACTTGGGGTCACCTTGATTGCTTATGCACCTTTAGCTCAAGGTAATTAATTCCACACCTAACACTAAATGTATTTTATAGAAAATTATTTCATTCACGTATATTCTGAGTCGCATCTTCAATGATTGATTTTTTTTTTATAAAGATACTTTAGGATAGCTGATGCAAGGATATCTCCACATGGGGGACATATCATAGCATCAGCTATAGTCCCACTTTATTAATAATAAAAGGTGATTATAAGAAGCGGGGGAATAGAAAGGCTAGAGGGCAGCCCGATTATCCCAAAACGAAAGCATGTAAATTTAAAGCAAAAGTATTAGACGAGTAGTTTTTGAAAAGTGAAGATCGGCTTGTCCATAGACCGGTTGTGGCTTGAATGTCTTCAATGATTGATGCAATATTAAATTCATATTCACCCACTAGCTTAAGTGTCTTTTGGGTCGGTCAAACTTCTTCATTATTGTTTTACTCCCAAATTAATATTCATTTCCACCTTGTTGGATTATATAGAATTAAATTCACCTTTACCCACTTAGGTTAAACTTTTGGATCGAGTTGGTGATTTAAGATACTCAATTTTTGCAGTTGAACTTAGTTAGTGGTTCGATTGGAGTTCTCAATAACAATTTTTTTTCTTTTTTTTTTTTTTATAAATATTTTTTAAATTTTTCTACCCTTACAAACCGAAAATGCACCCAAATATTGTTTGGATGCACATACACATTTTTATTCACTTTTATTTGGCAAATATATTTAGATTACTAATTAAAGGAGAGATATATACCTCACCAACAATTGTCTCTACTTTTCTTTTTCTTTTTACAATTTGGAAACTTTGGGATCTTTCTTCAACCTCTTCTTAAGAATATATTGAAAAACCTAAAATACATTAGTTAATTAAGTTTTTAAAAAATGTAAATAATTTTAATATGGTATTATGAGAATTAAGTAAAAAGAAAAAGTTATAATTTTGAATTTTGCCATGATTAAAAGATTTTGTCATTTTTATACATGTGAGTCAACCGTTGTTTTGTTGTCCTTCTCTTTGATTGAACATGATTCATATCTTATAACGATCGTCTAAATGAATTATTTGTTGAATTGTTTTCATTTTTAGATTTTAGATATTTTTCTTATTGAAAGCTTGGTTTCTTAATCACAGGCGTTCTAACGGGAAAGTACACACCAACAAATCCTCCAATTGGCCTTCGATCCCTAGTTTACTCTTCAGAGTTTTTAGCAAAGGTTAGAAGAAAAAAAAAATATTTTTTTAAATCTACGAAGAACAAAACAAAATTAAACAAGAAGGTACCACGGCATTTTCTATTTATTTGTGACTAAACCTTTTAATTAATTTACATGTGCAGCTTCAACCACTCTTGAATCTAATAAGGGACATAGGAGAGAAGTACAATAAATCCCCCACACTAGTTACTTTACTTTACTTCCTTTGTATATACAATTAACTATGTTTGTTCTTTTTTTATTTATTTGTTTTGTTGATGCGTGTTATCTCTACCTTTAGCATATCCGTTTTTAGAGCTTATTTGATAACTTTTCTGAATGGTATGTTTTAGTGTGTAACGAAGTAGATTGTTTAATTCATCGTGGTATCTAAAATATTGTAAAGAGATTAAATATAGGGGAGATCAATTTATGACACTCTTAGACTTTAGAGGTTTTCGTAGAAGATCCAAATAGTCATCTTTTACTCTTTGTGGATGTTAAATTCGAATGAAATTACTAAAAATGAAACCATCAAGTGAGCACGAATGCGCTCTCTTGCTTCGCTTTCTCTTGTTATGTTATTCGGTGCTTGCGCACTGTGCATGCAACACAACAGAAAAGTAAAGTGAGAGAGAAGAAAAAGTGGGCAGAAGAAGAAGAAGGGCTTGTCAAAGCTTGTGGAGTGGGTGTTGGGAGGGGAAACTTTTGATTTCGCTACAGATCAAGAAGAAGAGGGTGGAAGGGAAAAAGAGAAATATATATATGCAAAGATCAGGAAGAAAGAGAGAGAAAGAAAGGGTAGTTTTGTAAAGTTTGTTGATGATTATCATTTTATAACTTTCAATGAAGATAGTTTCAAATTCCAAAATTTAATGTTTGTGTGTGTAAATATTATTTTCATAAAGATGTAACCTGTACTGTTGGTTTAATTATTGATTAGGTGGTGTTGAACTGGTTGATAGCACAAGGAAATGTGGTGCCAATCCCTGGAGCCAAAAATGAAGAACAAGCCATTGAATTTGTTGGTGCTTTGGGTTGGAGAATTTCCAATGAAGAAATACACGAACTCCGCTCCTTCG

Coding sequence (CDS)

TTGCTCGAAATCCTTCTAATAAAAAACAATTCCAAGTTTTTCTTTAGATTCATTAAAGAAAGAAAGCGAAAGGATCCTGGATTTGAGATCACAATTGCTACCAAATATGCGGTTTTTCCATGGAGGCTCGGTCGTCACAGTGTCGTAAGTGTTCTCAAAGAATCACTTTGTCGTCTCGGTCTCGAATCGGTTGACTTGTATCAACTTCATTGGCCTGGAATATGGGGAAATGAAGAGTATATTGATGGTCTTGGAGATGCTGTTGAGCAAGGACTTGTGAAGGGTGTTGGTGTTTCTAATTATAATGAGAAGCGACTTCGAGATGCGTATGAAAAACTAAAGAAAAGAGGTATTCCACTAGCTTCAAATCAAGTGAATTATAGCCTCATATATAGATTACCTGAAGAGAACGGTGTGAAGGCTGCTTGTGATGAACTTGGGGTCACCTTGATTGCTTATGCACCTTTAGCTCAAGGCGTTCTAACGGGAAAGTACACACCAACAAATCCTCCAATTGGCCTTCGATCCCTAGTTTACTCTTCAGAGTTTTTAGCAAAGGTGGTGTTGAACTGGTTGATAGCACAAGGAAATGTGGTGCCAATCCCTGGAGCCAAAAATGAAGAACAAGCCATTGAATTTGTTGGTGCTTTGGGTTGGAGAATTTCCAATGAAGAAATACACGAACTCCGCTCCTTC

Protein sequence

LLEILLIKNNSKFFFRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF

Homology

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BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot
Match: Q94A68 (Uncharacterized oxidoreductase At1g06690, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g06690 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 8.7e-93
Identity = 161/237 (67.93%), Postives = 186/237 (78.48%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFI+ERK + PG E+++ATK+A  PWR GR SVV+ LK+SL RL L SVDLYQLHWPG+W
Sbjct: 125 RFIRERKERYPGAEVSVATKFAALPWRFGRESVVTALKDSLSRLELSSVDLYQLHWPGLW 184

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNE Y+DGLGDAVEQGLVK VGVSNY+EKRLRDAYE+LKKRGIPLASNQVNYSLIYR PE
Sbjct: 185 GNEGYLDGLGDAVEQGLVKAVGVSNYSEKRLRDAYERLKKRGIPLASNQVNYSLIYRAPE 244

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           + GVKAACDELGVTLIAY+P+AQG LTGKYTP NPP G R  +Y+ EFL K         
Sbjct: 245 QTGVKAACDELGVTLIAYSPIAQGALTGKYTPENPPSGPRGRIYTREFLTKLQPLLNRIK 304

Query: 196 ------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRS 232
                       + LNWL+AQGNV+PIPGAKN EQA EF GA+GW +++ E+ ELRS
Sbjct: 305 QIGENYSKTPTQIALNWLVAQGNVIPIPGAKNAEQAKEFAGAIGWSLTDNEVSELRS 361

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot
Match: Q56Y42 (Pyridoxal reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLR1 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 7.3e-39
Identity = 90/235 (38.30%), Postives = 136/235 (57.87%), Query Frame = 0


Query: 16  RFIKERK-RKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHW--P 75
           +FIKE +  K    E+ +ATK+A +PWRL     V+  + SL RL ++ + + QLHW   
Sbjct: 109 KFIKESQGLKGKQNEVVVATKFAAYPWRLTSGQFVNACRASLDRLQIDQLGIGQLHWSTA 168

Query: 76  GIWGNEEYI--DGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLI 135
                +E +  DGL    E+GLV+ VGVSNY  ++L   ++ LK RG+PL S QV +SL+
Sbjct: 169 SYAPLQELVLWDGLVQMYEKGLVRAVGVSNYGPQQLVKIHDYLKTRGVPLCSAQVQFSLL 228

Query: 136 YRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVY----------- 195
               E+  +K+ CDELG+ LI+Y+PL  G+LTGKY+ +  P G RSL++           
Sbjct: 229 SMGKEQLEIKSICDELGIRLISYSPLGLGMLTGKYSSSKLPTGPRSLLFRQILPGLEPLL 288

Query: 196 ---------SSEFLAKVVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEE 226
                      + + +V +NW I +G  VPIPG K+     + +GALGW+++N+E
Sbjct: 289 LALSEIAKKRGKTMPQVAINWCICKG-TVPIPGIKSVRHVEDNLGALGWKLTNDE 342

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BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot
Match: P77735 (1-deoxyxylulose-5-phosphate synthase YajO OS=Escherichia coli (strain K12) OX=83333 GN=yajO PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 6.0e-17
Identity = 70/233 (30.04%), Postives = 109/233 (46.78%), Query Frame = 0


Query: 29  EITIATK----YAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLH-WPGIWGNEEYIDG 88
           ++ +ATK        P  L R  ++  + +SL RLG++ VD+ Q+H W      EE ++ 
Sbjct: 82  DVVVATKVFHRVGDLPEGLSRAQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEA 141

Query: 89  LGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRG-IPLASNQVNYSLIYRLPEENGVKAA 148
           L D V+ G  + +G S+ +  +   A E  K+ G     S Q +Y+LIYR  EE  +   
Sbjct: 142 LNDVVKAGKARYIGASSMHASQFAQALELQKQHGWAQFVSMQDHYNLIYR-EEEREMLPL 201

Query: 149 CDELGVTLIAYAPLAQGVLTGKYTPT--------------------NPPIGLRSLVYSSE 208
           C + GV +I ++PLA+G LT  +  T                    +  I  R    S E
Sbjct: 202 CYQEGVAVIPWSPLARGRLTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEE 261

Query: 209 F---LAKVVLNWLIAQ-GNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRS 232
                A+V L WL+++ G   PI G   EEQ  E + A+   +  E+I EL +
Sbjct: 262 LGATRAQVALAWLLSKPGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELET 313

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BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot
Match: Q46851 (L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli (strain K12) OX=83333 GN=gpr PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-16
Identity = 72/227 (31.72%), Postives = 109/227 (48.02%), Query Frame = 0


Query: 29  EITIATK--YAVFPWRLG----RHSVVSVLKESLCRLGLESVDLYQLHWPGIWGN---EE 88
           E+ I+TK  Y ++P   G    R  +++ L +SL R+GLE VD++  H   +  N   EE
Sbjct: 91  ELIISTKAGYDMWPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSH--RVDENTPMEE 150

Query: 89  YIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPEENGV 148
               L  AV+ G    VG+S+Y+ +R +   E L++  IPL  +Q +Y+L+ R  +++G+
Sbjct: 151 TASALAHAVQSGKALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGL 210

Query: 149 KAACDELGVTLIAYAPLAQGVLTGKYTPTNP---------------------PIGLRSLV 208
                  GV  IA+ PLAQG+LTGKY    P                        L SL 
Sbjct: 211 LDTLQNNGVGCIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLR 270

Query: 209 YSSEF-------LAKVVLNWLIAQGNVVPI-PGAKNEEQAIEFVGAL 218
             +E        +A++ L+WL+    V  +  GA   EQ  E V AL
Sbjct: 271 LLNEMAQQRGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQAL 315

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BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot
Match: Q8X529 (L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli O157:H7 OX=83334 GN=gpr PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.6e-15
Identity = 54/146 (36.99%), Postives = 83/146 (56.85%), Query Frame = 0


Query: 29  EITIATK--YAVFPWRLG----RHSVVSVLKESLCRLGLESVDLYQLHWPGIWGN---EE 88
           E+ I+TK  Y ++P   G    R  +++ L +SL R+GLE VD++  H   +  N   EE
Sbjct: 91  ELIISTKAGYDMWPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSH--RVDENTPMEE 150

Query: 89  YIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPEENGV 148
               L  AV+ G    VG+S+Y+ +R +   E L +  IPL  +Q +Y+L+ R  +++G+
Sbjct: 151 TASALAHAVQSGKALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGL 210

Query: 149 KAACDELGVTLIAYAPLAQGVLTGKY 166
                  GV  IA+ PLAQG+LTGKY
Sbjct: 211 LDTLQNNGVGCIAFTPLAQGLLTGKY 234

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BLAST of Cucsat.G9024.T27 vs. NCBI nr
Match: XP_031739635.1 (uncharacterized oxidoreductase At1g06690, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 444 bits (1142), Expect = 5.12e-155
Identity = 216/218 (99.08%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 15  FRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI 74
           ++FIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI
Sbjct: 123 YQFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI 182

Query: 75  WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP 134
           WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP
Sbjct: 183 WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP 242

Query: 135 EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVVLNWLIA 194
           EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVVLNWLIA
Sbjct: 243 EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVVLNWLIA 302

Query: 195 QGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 232
           QGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF
Sbjct: 303 QGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 340

[Close]

BLAST of Cucsat.G9024.T27 vs. NCBI nr
Match: XP_031739634.1 (uncharacterized oxidoreductase At1g06690, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 433 bits (1113), Expect = 2.57e-150
Identity = 218/245 (88.98%), Postives = 219/245 (89.39%), Query Frame = 0


Query: 9   NNSKFFFRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ 68
           N+     RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ
Sbjct: 115 NSETLLGRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ 174

Query: 69  LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS 128
           LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS
Sbjct: 175 LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS 234

Query: 129 LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK-- 188
           LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK  
Sbjct: 235 LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKLQ 294

Query: 189 -------------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIH 232
                              VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIH
Sbjct: 295 PLLNLIRDIGEKYNKSPTLVVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIH 354

[Close]

BLAST of Cucsat.G9024.T27 vs. NCBI nr
Match: XP_031739633.1 (uncharacterized oxidoreductase At1g06690, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 432 bits (1110), Expect = 7.88e-150
Identity = 216/239 (90.38%), Postives = 218/239 (91.21%), Query Frame = 0


Query: 15  FRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI 74
           ++FIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI
Sbjct: 123 YQFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGI 182

Query: 75  WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP 134
           WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP
Sbjct: 183 WGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLP 242

Query: 135 EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK-------- 194
           EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK        
Sbjct: 243 EENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKLQPLLNLI 302

Query: 195 -------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 232
                        VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF
Sbjct: 303 RDIGEKYNKSPTLVVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 361

[Close]

BLAST of Cucsat.G9024.T27 vs. NCBI nr
Match: XP_016903626.1 (PREDICTED: uncharacterized oxidoreductase At1g06690, chloroplastic-like [Cucumis melo])

HSP 1 Score: 407 bits (1046), Expect = 9.72e-142
Identity = 202/238 (84.87%), Postives = 209/238 (87.82%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFIKE+K KDPGFE+T+ATKYAVFPWRLGRHSVVS LKESLCRLGLESVDLYQLHWPGIW
Sbjct: 16  RFIKEKKHKDPGFEVTVATKYAVFPWRLGRHSVVSALKESLCRLGLESVDLYQLHWPGIW 75

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNEEYIDGLGD VEQGLVK VGVSN  EKRLRDAYEKLKKRGIPLASNQVNYSLI+R+PE
Sbjct: 76  GNEEYIDGLGDPVEQGLVKAVGVSNNIEKRLRDAYEKLKKRGIPLASNQVNYSLIHRVPE 135

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSL+YSSEFLAK         
Sbjct: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLIYSSEFLAKLQPLLNLIR 195

Query: 196 ------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 232
                       V LNWLIAQGNVVPIPGAKNEEQAIEFVGALGW ISNEEIH+LRSF
Sbjct: 196 DIGEKYNKSPTQVALNWLIAQGNVVPIPGAKNEEQAIEFVGALGWTISNEEIHQLRSF 253

[Close]

BLAST of Cucsat.G9024.T27 vs. NCBI nr
Match: KAA0032881.1 (putative oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 396 bits (1017), Expect = 4.02e-137
Identity = 201/252 (79.76%), Postives = 208/252 (82.54%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFIKE+K KDPGFE+T+ATKYAVFPWRLGRHSVVS LKESLCRLGLESVDLYQLHWPGIW
Sbjct: 16  RFIKEKKHKDPGFEVTVATKYAVFPWRLGRHSVVSALKESLCRLGLESVDLYQLHWPGIW 75

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNEEYIDGLGD VEQGLVK VGVSN   KRLRDAYEKLKKRGIPLASNQVNYSLI+R+PE
Sbjct: 76  GNEEYIDGLGDPVEQGLVKAVGVSNIM-KRLRDAYEKLKKRGIPLASNQVNYSLIHRVPE 135

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSL+YSSEFLAK         
Sbjct: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLIYSSEFLAKLLYFYFLLI 195

Query: 196 --------------------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWR 232
                                     V LNWLIAQGNVVPIPGAKNEEQAIEFVGALGW 
Sbjct: 196 DLHVQLQPLLNLIRDIGEKYNKSPTQVALNWLIAQGNVVPIPGAKNEEQAIEFVGALGWT 255

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL
Match: A0A0A0KZ54 (Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G614230 PE=4 SV=1)

HSP 1 Score: 445 bits (1145), Expect = 8.07e-156
Identity = 218/224 (97.32%), Postives = 219/224 (97.77%), Query Frame = 0


Query: 9   NNSKFFFRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ 68
           N+     RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ
Sbjct: 115 NSETLLGRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ 174

Query: 69  LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS 128
           LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS
Sbjct: 175 LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS 234

Query: 129 LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVV 188
           LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVV
Sbjct: 235 LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVV 294

Query: 189 LNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 232
           LNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF
Sbjct: 295 LNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 338

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL
Match: A0A1S4E5V8 (uncharacterized oxidoreductase At1g06690, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103504123 PE=4 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 4.71e-142
Identity = 202/238 (84.87%), Postives = 209/238 (87.82%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFIKE+K KDPGFE+T+ATKYAVFPWRLGRHSVVS LKESLCRLGLESVDLYQLHWPGIW
Sbjct: 16  RFIKEKKHKDPGFEVTVATKYAVFPWRLGRHSVVSALKESLCRLGLESVDLYQLHWPGIW 75

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNEEYIDGLGD VEQGLVK VGVSN  EKRLRDAYEKLKKRGIPLASNQVNYSLI+R+PE
Sbjct: 76  GNEEYIDGLGDPVEQGLVKAVGVSNNIEKRLRDAYEKLKKRGIPLASNQVNYSLIHRVPE 135

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSL+YSSEFLAK         
Sbjct: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLIYSSEFLAKLQPLLNLIR 195

Query: 196 ------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRSF 232
                       V LNWLIAQGNVVPIPGAKNEEQAIEFVGALGW ISNEEIH+LRSF
Sbjct: 196 DIGEKYNKSPTQVALNWLIAQGNVVPIPGAKNEEQAIEFVGALGWTISNEEIHQLRSF 253

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL
Match: A0A5A7STM9 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold81G00470 PE=4 SV=1)

HSP 1 Score: 396 bits (1017), Expect = 1.95e-137
Identity = 201/252 (79.76%), Postives = 208/252 (82.54%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFIKE+K KDPGFE+T+ATKYAVFPWRLGRHSVVS LKESLCRLGLESVDLYQLHWPGIW
Sbjct: 16  RFIKEKKHKDPGFEVTVATKYAVFPWRLGRHSVVSALKESLCRLGLESVDLYQLHWPGIW 75

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNEEYIDGLGD VEQGLVK VGVSN   KRLRDAYEKLKKRGIPLASNQVNYSLI+R+PE
Sbjct: 76  GNEEYIDGLGDPVEQGLVKAVGVSNIM-KRLRDAYEKLKKRGIPLASNQVNYSLIHRVPE 135

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSL+YSSEFLAK         
Sbjct: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLIYSSEFLAKLLYFYFLLI 195

Query: 196 --------------------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVGALGWR 232
                                     V LNWLIAQGNVVPIPGAKNEEQAIEFVGALGW 
Sbjct: 196 DLHVQLQPLLNLIRDIGEKYNKSPTQVALNWLIAQGNVVPIPGAKNEEQAIEFVGALGWT 255

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL
Match: A0A6P6XJL3 (uncharacterized oxidoreductase At1g06690, chloroplastic-like isoform X4 OS=Coffea arabica OX=13443 GN=LOC113743374 PE=4 SV=1)

HSP 1 Score: 369 bits (946), Expect = 1.39e-125
Identity = 176/223 (78.92%), Postives = 191/223 (85.65%), Query Frame = 0


Query: 9   NNSKFFFRFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQ 68
           N+     RFIKERK ++P  E+ +ATK+A  PWR GR SV++ LK+SLCRLGL SVDLYQ
Sbjct: 113 NSETLLGRFIKERKERNPQVEVAVATKFAALPWRFGRQSVLTALKDSLCRLGLSSVDLYQ 172

Query: 69  LHWPGIWGNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYS 128
           LHWPGIWGNE YIDGLGDAVEQGLVK VGVSNY EKRLRDAYE+LKKRGIPLASNQVNYS
Sbjct: 173 LHWPGIWGNEGYIDGLGDAVEQGLVKAVGVSNYTEKRLRDAYEQLKKRGIPLASNQVNYS 232

Query: 129 LIYRLPEENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAKVV 188
           LIYR PEENGVKAACDELGVTLIAY+P+AQG LTGKYTP NPP G R  +Y+SEFL KVV
Sbjct: 233 LIYRAPEENGVKAACDELGVTLIAYSPIAQGALTGKYTPENPPSGPRGRIYTSEFLVKVV 292

Query: 189 LNWLIAQGNVVPIPGAKNEEQAIEFVGALGWRISNEEIHELRS 231
           LNWLIAQ NVVPIPGAKN EQA EF GALGWR+ NEEI ELRS
Sbjct: 293 LNWLIAQENVVPIPGAKNAEQAKEFAGALGWRLGNEEIDELRS 335

[Close]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL
Match: A0A5D3CGA2 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504G00260 PE=4 SV=1)

HSP 1 Score: 363 bits (931), Expect = 1.89e-123
Identity = 185/235 (78.72%), Postives = 191/235 (81.28%), Query Frame = 0


Query: 16  RFIKERKRKDPGFEITIATKYAVFPWRLGRHSVVSVLKESLCRLGLESVDLYQLHWPGIW 75
           RFIKE+K KDPGFE+T+ATKYAVFPWRLGRHSVVS LKESLCRLGLESVDLYQLHWPGIW
Sbjct: 16  RFIKEKKHKDPGFEVTVATKYAVFPWRLGRHSVVSALKESLCRLGLESVDLYQLHWPGIW 75

Query: 76  GNEEYIDGLGDAVEQGLVKGVGVSNYNEKRLRDAYEKLKKRGIPLASNQVNYSLIYRLPE 135
           GNEEYIDGLGD VEQGLVK VGVSN   KRLRDAYEKLKKRGIPLASNQVNYSLI+R+PE
Sbjct: 76  GNEEYIDGLGDPVEQGLVKAVGVSNIM-KRLRDAYEKLKKRGIPLASNQVNYSLIHRVPE 135

Query: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLVYSSEFLAK--------- 195
           ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSL+YSSEFLAK         
Sbjct: 136 ENGVKAACDELGVTLIAYAPLAQGVLTGKYTPTNPPIGLRSLIYSSEFLAKLLYFYFLLI 195

Query: 196 --------------------------VVLNWLIAQGNVVPIPGAKNEEQAIEFVG 215
                                     V LNWLIAQGNVVPIPGAKNEEQAIEF G
Sbjct: 196 DLHVQLQPLLNLIRDIGEKYNKSPTQVALNWLIAQGNVVPIPGAKNEEQAIEFSG 249

The following BLAST results are available for this feature:

BLAST of Cucsat.G9024.T27 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94A68	8.7e-93	67.93	Uncharacterized oxidoreductase At1g06690, chloroplastic OS=Arabidopsis thaliana ...	[more]
Q56Y42	7.3e-39	38.30	Pyridoxal reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLR1 PE=1 ...	[more]
P77735	6.0e-17	30.04	1-deoxyxylulose-5-phosphate synthase YajO OS=Escherichia coli (strain K12) OX=83...	[more]
Q46851	1.7e-16	31.72	L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli (strain K12) OX=83333...	[more]
Q8X529	5.6e-15	36.99	L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli O157:H7 OX=83334 GN=g...	[more]

BLAST of Cucsat.G9024.T27 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739635.1	5.12e-155	99.08	uncharacterized oxidoreductase At1g06690, chloroplastic isoform X3 [Cucumis sati...	[more]
XP_031739634.1	2.57e-150	88.98	uncharacterized oxidoreductase At1g06690, chloroplastic isoform X2 [Cucumis sati...	[more]
XP_031739633.1	7.88e-150	90.38	uncharacterized oxidoreductase At1g06690, chloroplastic isoform X1 [Cucumis sati...	[more]
XP_016903626.1	9.72e-142	84.87	PREDICTED: uncharacterized oxidoreductase At1g06690, chloroplastic-like [Cucumis...	[more]
KAA0032881.1	4.02e-137	79.76	putative oxidoreductase [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G9024.T27 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ54	8.07e-156	97.32	Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G61423...	[more]
A0A1S4E5V8	4.71e-142	84.87	uncharacterized oxidoreductase At1g06690, chloroplastic-like OS=Cucumis melo OX=...	[more]
A0A5A7STM9	1.95e-137	79.76	Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A6P6XJL3	1.39e-125	78.92	uncharacterized oxidoreductase At1g06690, chloroplastic-like isoform X4 OS=Coffe...	[more]
A0A5D3CGA2	1.89e-123	78.72	Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020471	Aldo-keto reductase	PRINTS	PR00069	ALDKETRDTASE	coord: 54..72 score: 50.53 coord: 84..101 score: 42.03
IPR023210	NADP-dependent oxidoreductase domain	PFAM	PF00248	Aldo_ket_red	coord: 16..170 e-value: 4.5E-32 score: 111.4
IPR036812	NADP-dependent oxidoreductase domain superfamily	GENE3D	3.20.20.100		coord: 8..232 e-value: 1.1E-51 score: 177.8
IPR036812	NADP-dependent oxidoreductase domain superfamily	SUPERFAMILY	51430	NAD(P)-linked oxidoreductase	coord: 16..231
None	No IPR available	PANTHER	PTHR43625:SF22	OS07G0143000 PROTEIN	coord: 13..231
None	No IPR available	PANTHER	PTHR43625	AFLATOXIN B1 ALDEHYDE REDUCTASE	coord: 13..231
None	No IPR available	CDD	cd19093	AKR_AtPLR-like	coord: 16..230 e-value: 4.38602E-110 score: 316.092
IPR018170	Aldo/keto reductase, conserved site	PROSITE	PS00062	ALDOKETO_REDUCTASE_2	coord: 84..101

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G9024	Cucsat.G9024	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9024.T27.E1	Cucsat.G9024.T27.E1	exon
Cucsat.G9024.T27.E2	Cucsat.G9024.T27.E2	exon
Cucsat.G9024.T27.E3	Cucsat.G9024.T27.E3	exon
Cucsat.G9024.T27.E4	Cucsat.G9024.T27.E4	exon
Cucsat.G9024.T27.E5	Cucsat.G9024.T27.E5	exon
Cucsat.G9024.T27.E6	Cucsat.G9024.T27.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9024.T27.C1	Cucsat.G9024.T27.C1	CDS
Cucsat.G9024.T27.C2	Cucsat.G9024.T27.C2	CDS
Cucsat.G9024.T27.C3	Cucsat.G9024.T27.C3	CDS
Cucsat.G9024.T27.C4	Cucsat.G9024.T27.C4	CDS
Cucsat.G9024.T27.C5	Cucsat.G9024.T27.C5	CDS
Cucsat.G9024.T27.C6	Cucsat.G9024.T27.C6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9024.T27	Cucsat.G9024.T27-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005829	cytosol
molecular_function	GO:0047834	D-threo-aldose 1-dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0004032	alditol:NADP+ 1-oxidoreductase activity
molecular_function	GO:0004033	aldo-keto reductase (NADP) activity