Cucsat.G8930.T11 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8930.T11
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	sodium/calcium exchanger NCL-like
Location	ctg1635: 2043390 .. 2047475 (-)
RNA-Seq Expression	Cucsat.G8930.T11
Synteny	Cucsat.G8930.T11

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCTTTTGCACTTTCATGGGTTTGACCTGTAATCGAAATGTCCTGCGAAATGAAATGGGTTGGCTTATACCTTTTCTCAAGAACATGATCGTTTTGATTTGTGAATTTGAGGTGGGGTTCTGTTTCTGTGATCCAGGCAAAGGCTATATAGATTAAGGTTCTGTGGATGATCCATATCTTACTTTCGTTTTTCGTTTGTTCATTTCCCCATGAACTATTTGATCGTAGAAACTACATAAACGCGGTGGGAATAGAATTAGAATGGTGAAATGTATAGTGGAGTTCTCTTTTGTTTTTTCTTGGCTACTTTTTCCAAAAAAAAAGTTCCGTTTATTGACCCCCCAAAAGTTTCCTAAACGGATCGAGTATTGTAAAATAAAGGTGGGGGTGCCTTGTTATTGAAGTTCTATTTGCTATGATATCTCTACTTTGCTTTGTTTGATTTTTGCTTGGTGTGGGCATCTCTCTGCTTTTGCTGAGCTCGCTTTTCTGGCGATTCCTTGGTTCATCAAACTTTATTGTACTTCAATTTTTGCTGCTTGTCGGTTATATCTCAACATCAAGAACCTGAACGATTCTCATCTCTTTTTTTTTCCAAGACCGACATCTTTTCAAAAACCCATCACTTAGTTCCCAAACTGGGCAAGATTTATTCCAAAATTTCAGTTTAGCCATTACTCTATGTTTTCAAAATGGACCATCCTCTAATATCTGTTGTCTTTTTGTTTCTTGAGCCGTTTCTAAATGATAGGTTATGTAATTCTGAACTCCATATTTTTTGGGTTCATATTTTACATCGATTTTCGTTGTGGCAAAATGATATCTAGACTTTCACCAATAGAAGTTAGTTAAATGAGCTTAATCGAATATGAAGAGTGATAAAAAGCACCGAGTTCTTAAGCTTGAATTGGCCACACTCATTGGAATGTCTACTGTTTTGATTTCTCTTGTTTCAGTTTGTTTGTGACGAATGAGAGATCTGAAAAAAACCCTCTAATCTTCTCCTTTCTATAGTTTCTGGTCTTGCAGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCTGTTGGAATGGGTCTACTAGCTGGCTCAACTGTCATGCTTCTTACACTGATCTGGGGCACATGTGTGATTGTTGGGAAGTGTGACCTTCAAGATTCAGTTGCCATAGATTCACAGGATACAAAAGGGTTTAGCTTGACTGGTATGTCATTTTATCTTAGTTATATTCAACTGAGGTGTCCGATCGTGTTCATGCATGCCTTCGTTTTAGAAAGTTGAATCTTATTCAGGGGAGCGTGATGCTTTCTTATGGTGTGGAAACAAGGATGATGCTATTCGTAATGGATCAATACTTTTTGATCAATAATACACCCTTTTTTTTCATCAAGGCTTACAAAGGAAAAGGACATATTTTGAATAGTATTTAAGGTGTTATGTGAGTTCTGTCATTGACTACAGGCAACTTCTATTGTTTTAGCCGTGACTTGTGACAACGACAATTTATCGTGTTAATCTACTCTTCATATTGCCTAGAAACTGAATATTAGGTAAACTTGGTGAAACTACTTGCAAGCATCAATTAAAGCGTAACGTAGTCGTACATGCTGGGAAGACCCCTAGTTTTTCACCTTATGAGCATACCTGAAGCTGTTAGTACACGGTTGCCTATTAATGCTTCATCATTGCACTTCCATACTTCCTATTGATCTCTTTCTGATTGTCAACTATTCTCAGTCAATGGTCCATTTTCTCATACGTGCTATTGGATCAACTGAATTGGATTTTAATTTCCGAATGTTATATGGTTTTTAAGTTCTGTGGATACTTCGATGCATAAATTTACGTTGAAATTTTTTCTTTGACAGAATCTGGAGTTAGCACTGATATTTGGACCAGCTATGCAGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTGATCGTTCAACTTCCACAAATGCTCAATTCAACATCAGGAAGGCACTTGGCAGTTTTGATTGCTCTTATTATCTCTGTTTCAATGTTCATTATCTATTGCCTTTACCAGGTATTTTTTTCTATTTCTTAGCTCATTTTTTATTAAGTTTACCCAAAATTTTATTTTCATTTTTTTGAATTTGTTGTCTACACAACATTGGAAAATTAAAAAGACCCACATTTACAGATTTTTCATGTTTTAAACTTTTAAACACAAATGCTAACTCAAGTAAGCTCACCTTCATTCAAACACCGAATTTGGTGAGAAAGAAACATAGGTAGCTACTCTAATCTTCCAGAGTTCAAAAGTTATAGCTTCAACGGAAGGATCGATACCTTCACAATGTATTAAATTTATTCTATTAAACTTGATGTTTACCTTTTACTTTAAGGATAAGACTTATTTCGTTCTATTTTTTCTCTCATGTAAATGTTTGAAATCTTACTTCAATGATATCCTACTTCATGAATTCGAGAAAATTTGTGCAGGTCTTCCAACCATGGATTCAAAGGAGGAAACTTGCTTTTGTCAAGCACAAGCATGTTATATTTGGATTTTTAAGACATTTAAAACAGCAAACACTTGGAAGACTCCTGACAGAGAATGGGGAGCCAGATAAAGAAATAATAGAGAAGTTCGTTGGAACTAGAAGTTCAATTGTTCATTTTTATCATCTCCTTTTTATTTTATCATATGCTGATAGTTGCTCAATGAACATAATGACAGGTTATTTTCAAGAATTGATGTGAACAAGGATGGACTTCTATCAGCTTCTGAACTGAGAGCACTAATAGTTGGCATTCAGTTTGACGAGATGGACTTGGATCATGATGATGCTGTTGACAAAATAATGAATGACTTTGATACTTCTCGTGATTCCCATGTTGATTCTAACGAGTTTGGCAATGGAATCATTAGGTGGCTTTCTCAAGTTCAAGGTTCAAGAACTGGCAGAGGTGAGGATGGTCCTCACACAATGAAGTACCTGCATAACTTTCATCAAGTAAGTTGTAAGCTGTTAGCTGTATTTCTCCTTTCAATTTTTAGTTTCGCTTTTCTGAAGCATGTCATGATGTATCTACAGGAAACAAAGAGAGAACACGATTTGCTGGATGTTGGGGAACAGAGCGATGAGGTCGTAGAGGGCGTCGAGGAGGGGAAGGGGGTCTTGATAAAAGCTATACTCTTCTTGTTACTTGGAACCGCCATTGCTGCTGCCTTTGCTGACCCTTTGGTTGACGTCGTACATAACTTCTCTAATGCAACTAAAATCCCTGCGTTCTTTATTTCATTTATTGCTCTGCCATTAGCAACCAACTCAAGTGAAGCAGTCTCTGCTATTATCTTTGCAAGCCGTGACAAGCGAAAAACTGCCTCATTAACATTTTCCGAGGTTTGTCTTCTTTTATACTCAACATTTTGTTTCTTTACTCAGTTAATTTGTACTGGGGACCAGTTGAAAGGAAAACTGGAAGAAATTGGAAGAATGATTGACCTCTTACCTATAAGCTTTCATGGTGCAGCTATATGGAGCAGTTACAATGAACAATGTCCTCTGCCTATCAGTTTTCTTGGCTCTTGTTTACATGAGAGGATTGGTTTGGAACTTTTCATCTGAAGTGCTGGTCATTCTCATCGTTACAATGATAATGGGAGTCATGGGTAGCTTCCGAACCGCCTTCCCTCTCTGGACATCTCTGGTGGCTCTCCTTCTCTACCCCCTCTCTCTTGTGTTGGTCTATGTTCTTGATTATGTCTTTGGTTGGTCATAGATCAGTCGGCATTTTGAGTTTTATTCAGGGGGTTTGTTCCTTTAAATTTCCGGATGTTTTTGCACATACTCTCTGCTGAGCCAATGTTATACAATAACTGTTCTGCTCTCTTTTTATTTGCTTCATTTTTGTGTTGGTATTGATATTGAAAGTGAAGTAAAATAATGAGCATAATTTGTTTATCGAATTATCTAATAAAAAAGTTGGGGGTCATGGTGAACTCAAAAAGAAATTATATATATATGTTCTATGCATCATTTCACGTTGAATTCTCTAAGAATTGTATGTTTCTTTTAATTATTATGTTTTGGCCTTGGTTAATTTCTCGAAGGTTT

Coding sequence (CDS)

ATGATATCCTACTTCATGAATTCGAGAAAATTTGTGCAGGTCTTCCAACCATGGATTCAAAGGAGGAAACTTGCTTTTGTCAAGCACAAGCATGTTATATTTGGATTTTTAAGACATTTAAAACAGCAAACACTTGGAAGACTCCTGACAGAGAATGGGGAGCCAGATAAAGAAATAATAGAGAAGTTATTTTCAAGAATTGATGTGAACAAGGATGGACTTCTATCAGCTTCTGAACTGAGAGCACTAATAGTTGGCATTCAGTTTGACGAGATGGACTTGGATCATGATGATGCTGTTGACAAAATAATGAATGACTTTGATACTTCTCGTGATTCCCATGTTGATTCTAACGAGTTTGGCAATGGAATCATTAGGTGGCTTTCTCAAGTTCAAGGTTCAAGAACTGGCAGAGGTGAGGATGGTCCTCACACAATGAAGTACCTGCATAACTTTCATCAAGAAACAAAGAGAGAACACGATTTGCTGGATGTTGGGGAACAGAGCGATGAGGTCGTAGAGGGCGTCGAGGAGGGGAAGGGGGTCTTGATAAAAGCTATACTCTTCTTGTTACTTGGAACCGCCATTGCTGCTGCCTTTGCTGACCCTTTGGTTGACGTCGTACATAACTTCTCTAATGCAACTAAAATCCCTGCGTTCTTTATTTCATTTATTGCTCTGCCATTAGCAACCAACTCAAGTGAAGCAGTCTCTGCTATTATCTTTGCAAGCCGTGACAAGCGAAAAACTGCCTCATTAACATTTTCCGAGCTATATGGAGCAGTTACAATGAACAATGTCCTCTGCCTATCAGTTTTCTTGGCTCTTGTTTACATGAGAGGATTGGTTTGGAACTTTTCATCTGAAGTGCTGGTCATTCTCATCGTTACAATGATAATGGGAGTCATGGGTAGCTTCCGAACCGCCTTCCCTCTCTGGACATCTCTGGTGGCTCTCCTTCTCTACCCCCTCTCTCTTGTGTTGGTCTATGTTCTTGATTATGTCTTTGGTTGGTCATAG

Protein sequence

MISYFMNSRKFVQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQGSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS

Homology

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BLAST of Cucsat.G8930.T11 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 6.5e-113
Identity = 220/329 (66.87%), Postives = 251/329 (76.29%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRR+LAF KHKHVI G LRHLKQ  LGRLL + G+PD+ +I KLF  ID N D
Sbjct: 257 QVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKLFLTIDANND 316

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           G LSA+EL+ALI+GI F+++D D DDAV K++ DFD + D  VD  EF  GI +WL Q  
Sbjct: 317 GHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGIKQWLIQAM 376

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEG--VEEGKGVLIKAILFL 192
           G      E GP TMK+L NFH +TKREH LL   E  +   EG  V + K + IKA L L
Sbjct: 377 GGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKWITIKAALLL 436

Query: 193 LLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKT 252
           LLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAIIFASR K +T
Sbjct: 437 LLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAIIFASRKKIRT 496

Query: 253 ASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAF 312
           ASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVILIV ++MG   SFRT +
Sbjct: 497 ASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMGGFASFRTTY 556

Query: 313 PLWTSLVALLLYPLSLVLVYVLDYVFGWS 340
           PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 557 PLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

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BLAST of Cucsat.G8930.T11 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 9.1e-107
Identity = 205/327 (62.69%), Postives = 261/327 (79.82%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQ+RKLA+ KHKHVI G LRH + + LGRLL E+G P++++I+KLF +ID+++ 
Sbjct: 263 QVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLNEDGTPNEDVIKKLFHKIDMDES 322

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
             LS +EL ALI+GI F+E+D D +DAVDKIM+DFDTS +  V+  EF +G+ RWL++ +
Sbjct: 323 QTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEEAEFVSGMKRWLNEAK 382

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
            S       G ++ K++ ++H  T++EHDLL   ++SDE VE VE     + KA+  LLL
Sbjct: 383 RSVP---TSGAYSNKFITDYHARTRQEHDLL--VDRSDETVESVENPGWCITKAVGLLLL 442

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           G+AIAAAFADPLVD VHNFSNA+ IP+FFISFIALPLATNSSEAVSAIIFASR K +T+S
Sbjct: 443 GSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSSEAVSAIIFASRKKLRTSS 502

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL+V +IMG+  SFRT FPL
Sbjct: 503 LTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIILLVCVIMGLFTSFRTTFPL 562

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 340
           WT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 563 WTCLVAYMLYPLSLVVVYILDFVFGWS 584

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BLAST of Cucsat.G8930.T11 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 362.5 bits (929), Expect = 5.4e-99
Identity = 197/330 (59.70%), Postives = 249/330 (75.45%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRR+L + + KHV+ G LRH ++ ++GRLL + G P+  +IEKLF RID + D
Sbjct: 255 QVFQPWIQRRRLEYTRLKHVMSGLLRHAQKHSIGRLLDDEGRPNVSVIEKLFHRIDQDND 314

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           G L   EL+A IVGI F+++D + + A D++M DFDTSR+  ++  EF NG++RWL + +
Sbjct: 315 GKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRNHFIEKGEFVNGMLRWLDEAK 374

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREH-DLLDVGEQSDEVVEGVEEGK--GVLIKAILF 192
            + T     G ++ K+L++FH  T+ E   LLD  E+     EG  +G      IKAIL 
Sbjct: 375 RTVT----SGAYSKKFLNDFHARTRDEQTGLLDKDEE-----EGEADGNPTWTCIKAILL 434

Query: 193 LLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRK 252
           LLLGTA+AAA ADPLVD VHNFSNAT IP+FFISFI +PLATNSSEAVSAIIFASR K++
Sbjct: 435 LLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLATNSSEAVSAIIFASRKKKR 494

Query: 253 TASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTA 312
           T SLTFSE+YG VTMNN LCL+VFLALVY+RGL W+FSSEVL+IL+V +IMG+  SFRT 
Sbjct: 495 TLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVLIILLVCIIMGLFTSFRTD 554

Query: 313 FPLWTSLVALLLYPLSLVLVYVLDYVFGWS 340
           FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 555 FPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

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BLAST of Cucsat.G8930.T11 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 5.0e-04
Identity = 41/139 (29.50%), Postives = 71/139 (51.08%), Query Frame = 0


Query: 165 VGEQSDEVVEGVEEGKGVLI-KAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFIS 224
           +GEQ +EV E  EE K +   +AI +L + T   +  +  LVD +   S +  +P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 225 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRG-- 284
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 285 LVWNFSSEVLVILIVTMIM 301
           +  NF       L +T+++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

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BLAST of Cucsat.G8930.T11 vs. NCBI nr
Match: XP_004146032.1 (sodium/calcium exchanger NCL [Cucumis sativus] >KGN55087.1 hypothetical protein Csa_012240 [Cucumis sativus])

HSP 1 Score: 628 bits (1620), Expect = 2.81e-222
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 263 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 322

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 323 GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 382

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 383 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 442

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 443 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 502

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL
Sbjct: 503 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 562

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 563 WTSLVALLLYPLSLVLVYVLDYVFGWS 589

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BLAST of Cucsat.G8930.T11 vs. NCBI nr
Match: ADN33808.1 (sodium/calcium exchanger family protein [Cucumis melo subsp. melo])

HSP 1 Score: 620 bits (1600), Expect = 2.68e-219
Identity = 320/327 (97.86%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 259 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 318

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDE+DLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 319 GLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 378

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRG+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 379 GSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 438

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 439 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 498

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT++MGVMGSFRTAFPL
Sbjct: 499 LTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTVVMGVMGSFRTAFPL 558

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 559 WTSLVALLLYPFSLVLVYVLDYVFGWS 585

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BLAST of Cucsat.G8930.T11 vs. NCBI nr
Match: XP_008463718.1 (PREDICTED: uncharacterized protein LOC103501799 [Cucumis melo] >KAA0066783.1 sodium/calcium exchanger family protein [Cucumis melo var. makuwa] >TYK27930.1 sodium/calcium exchanger family protein [Cucumis melo var. makuwa])

HSP 1 Score: 620 bits (1600), Expect = 3.19e-219
Identity = 320/327 (97.86%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 264 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 323

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDE+DLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 324 GLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 383

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRG+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 384 GSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 443

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 444 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 503

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT++MGVMGSFRTAFPL
Sbjct: 504 LTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTVVMGVMGSFRTAFPL 563

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 564 WTSLVALLLYPFSLVLVYVLDYVFGWS 590

[Close]

BLAST of Cucsat.G8930.T11 vs. NCBI nr
Match: XP_038897650.1 (sodium/calcium exchanger NCL [Benincasa hispida])

HSP 1 Score: 592 bits (1527), Expect = 3.54e-208
Identity = 305/327 (93.27%), Postives = 320/327 (97.86%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ+LGRLL ENGEPDKEII+KLFS ID NKD
Sbjct: 261 QVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQSLGRLLRENGEPDKEIIDKLFSSIDENKD 320

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQF+E+DLDHDDAV+KIMNDFDTSRDSHV++ EFGNGIIRWL+QVQ
Sbjct: 321 GLLSASELRALIVGIQFEEIDLDHDDAVNKIMNDFDTSRDSHVNATEFGNGIIRWLTQVQ 380

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSR G+G+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 381 GSRPGKGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 440

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 441 GTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 500

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT+IMGV+GSFRTAFPL
Sbjct: 501 LTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVLVILVVTIIMGVLGSFRTAFPL 560

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 561 WTALVALLLYPLSLVLVYVLDYVFGWS 587

[Close]

BLAST of Cucsat.G8930.T11 vs. NCBI nr
Match: KAG6591925.1 (Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589 bits (1518), Expect = 3.86e-207
Identity = 303/327 (92.66%), Postives = 318/327 (97.25%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLTENGEPDKEII+KLFSRID NKD
Sbjct: 239 QVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKLFSRIDENKD 298

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           G LSASELRALIVGIQF+E+DLDH+DAV+KI+NDFDTSRDSHVD+ EFGNGII+WLSQVQ
Sbjct: 299 GRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEFGNGIIKWLSQVQ 358

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGR +DGPHTMK+LHNFHQETKREHDLLDVGEQSDEVVE VE GKGVLIKAILFLLL
Sbjct: 359 GSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEGGKGVLIKAILFLLL 418

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 419 GTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 478

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL+VT+IMG++GSFRTAFPL
Sbjct: 479 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGLLGSFRTAFPL 538

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 539 WTALVALLLYPLSLVLVYVLDYVFGWS 565

[Close]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL
Match: A0A0A0L3B8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G628350 PE=4 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 1.36e-222
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 263 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 322

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 323 GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 382

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 383 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 442

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 443 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 502

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL
Sbjct: 503 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 562

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 563 WTSLVALLLYPLSLVLVYVLDYVFGWS 589

[Close]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL
Match: E5GBG6 (Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 620 bits (1600), Expect = 1.30e-219
Identity = 320/327 (97.86%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 259 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 318

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDE+DLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 319 GLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 378

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRG+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 379 GSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 438

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 439 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 498

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT++MGVMGSFRTAFPL
Sbjct: 499 LTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTVVMGVMGSFRTAFPL 558

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 559 WTSLVALLLYPFSLVLVYVLDYVFGWS 585

[Close]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 620 bits (1600), Expect = 1.54e-219
Identity = 320/327 (97.86%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 264 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 323

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDE+DLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 324 GLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 383

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRG+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 384 GSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 443

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 444 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 503

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT++MGVMGSFRTAFPL
Sbjct: 504 LTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTVVMGVMGSFRTAFPL 563

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 564 WTSLVALLLYPFSLVLVYVLDYVFGWS 590

[Close]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL
Match: A0A1S3CJX3 (uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=4 SV=1)

HSP 1 Score: 620 bits (1600), Expect = 1.54e-219
Identity = 320/327 (97.86%), Postives = 326/327 (99.69%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD
Sbjct: 264 QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 323

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           GLLSASELRALIVGIQFDE+DLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ
Sbjct: 324 GLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 383

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGRG+DGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL
Sbjct: 384 GSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 443

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 444 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 503

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL+VT++MGVMGSFRTAFPL
Sbjct: 504 LTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTVVMGVMGSFRTAFPL 563

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 564 WTSLVALLLYPFSLVLVYVLDYVFGWS 590

[Close]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL
Match: A0A6J1IGZ1 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE=4 SV=1)

HSP 1 Score: 589 bits (1518), Expect = 3.24e-207
Identity = 303/327 (92.66%), Postives = 318/327 (97.25%), Query Frame = 0


Query: 13  QVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENGEPDKEIIEKLFSRIDVNKD 72
           QVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLLTENGEPDKEII+KLFSRID NKD
Sbjct: 255 QVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKLFSRIDENKD 314

Query: 73  GLLSASELRALIVGIQFDEMDLDHDDAVDKIMNDFDTSRDSHVDSNEFGNGIIRWLSQVQ 132
           G LSASELRALIVGIQF+E+DLDH+DAV+KI+NDFDTSRDSHVD+ EFGNGII+WLSQVQ
Sbjct: 315 GRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEFGNGIIKWLSQVQ 374

Query: 133 GSRTGRGEDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEEGKGVLIKAILFLLL 192
           GSRTGR +DGPHTMK+LHNFHQETKREHDLLDVGEQSDEVVE VE GKGVLIKAILFLLL
Sbjct: 375 GSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEGGKGVLIKAILFLLL 434

Query: 193 GTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 252
           GTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS
Sbjct: 435 GTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTAS 494

Query: 253 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILIVTMIMGVMGSFRTAFPL 312
           LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL+VT+IMG++GSFRTAFPL
Sbjct: 495 LTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGLLGSFRTAFPL 554

Query: 313 WTSLVALLLYPLSLVLVYVLDYVFGWS 339
           WT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 555 WTALVALLLYPLSLVLVYVLDYVFGWS 581

The following BLAST results are available for this feature:

BLAST of Cucsat.G8930.T11 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L636	6.5e-113	66.87	Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1	[more]
Q5QNI2	9.1e-107	62.69	Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 P...	[more]
Q6K3R5	5.4e-99	59.70	Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 P...	[more]
Q5KQN0	5.0e-04	29.50	Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C...	[more]

BLAST of Cucsat.G8930.T11 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146032.1	2.81e-222	100.00	sodium/calcium exchanger NCL [Cucumis sativus] >KGN55087.1 hypothetical protein ...	[more]
ADN33808.1	2.68e-219	97.86	sodium/calcium exchanger family protein [Cucumis melo subsp. melo]	[more]
XP_008463718.1	3.19e-219	97.86	PREDICTED: uncharacterized protein LOC103501799 [Cucumis melo] >KAA0066783.1 sod...	[more]
XP_038897650.1	3.54e-208	93.27	sodium/calcium exchanger NCL [Benincasa hispida]	[more]
KAG6591925.1	3.86e-207	92.66	Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G8930.T11 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3B8	1.36e-222	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G628350 PE=4 SV=1	[more]
E5GBG6	1.30e-219	97.86	Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE...	[more]
A0A5A7VFE4	1.54e-219	97.86	Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CJX3	1.54e-219	97.86	uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=...	[more]
A0A6J1IGZ1	3.24e-207	92.66	sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 99..127 e-value: 6.6 score: 11.1 coord: 59..87 e-value: 0.061 score: 22.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 60..120 e-value: 2.2E-7 score: 31.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 55..90 score: 12.588623
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 95..130 score: 8.822743
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 60..120 e-value: 2.06043E-7 score: 45.6165
None	No IPR available	GENE3D	1.10.238.10		coord: 10..134 e-value: 8.4E-12 score: 47.3
None	No IPR available	PANTHER	PTHR31503:SF36	SODIUM/CALCIUM EXCHANGER NCL	coord: 9..336
IPR004837	Sodium/calcium exchanger membrane region	PFAM	PF01699	Na_Ca_ex	coord: 187..330 e-value: 1.2E-11 score: 44.7
IPR004713	Calcium/proton exchanger	PANTHER	PTHR31503	VACUOLAR CALCIUM ION TRANSPORTER	coord: 9..336
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 68..80
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 41..265

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8930	Cucsat.G8930	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8930.T11.E1	Cucsat.G8930.T11.E1	exon
Cucsat.G8930.T11.E2	Cucsat.G8930.T11.E2	exon
Cucsat.G8930.T11.E3	Cucsat.G8930.T11.E3	exon
Cucsat.G8930.T11.E4	Cucsat.G8930.T11.E4	exon
Cucsat.G8930.T11.E5	Cucsat.G8930.T11.E5	exon
Cucsat.G8930.T11.E6	Cucsat.G8930.T11.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8930.T11.C4	Cucsat.G8930.T11.C4	CDS
Cucsat.G8930.T11.C3	Cucsat.G8930.T11.C3	CDS
Cucsat.G8930.T11.C2	Cucsat.G8930.T11.C2	CDS
Cucsat.G8930.T11.C1	Cucsat.G8930.T11.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8930.T11	Cucsat.G8930.T11-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006812	cation transport
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0070588	calcium ion transmembrane transport
biological_process	GO:0006874	cellular calcium ion homeostasis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0008324	cation transmembrane transporter activity
molecular_function	GO:0015369	calcium:proton antiporter activity