Cucsat.G8906.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8906.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Sodium-bile acid cotransporter
Location	ctg1635: 1620101 .. 1623812 (-)
RNA-Seq Expression	Cucsat.G8906.T2
Synteny	Cucsat.G8906.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAGTGACGAAGGGAACAGAAAGCTTTAGGTTTTGACATCGAATCAATTGACCAAAATATTACATTAACGTCAAAAATGGTTTCCCCGGTCCCATTGCCACTCTCCACCGCTCCACTTAACCAAGCTGCCCAAAGGGTTTTGGCGGGATTCAACATCGCCGTCACCGGAGTTTCCCCGGTTCTGGAAACACTGGGCTGAGCTGAGCTTCACACTCACCTCATTGTCGAAGTTCCATTCATTCTTCTTTTGGTCGTCGCCGACACACACAGAATGCAGTCTCTTCCATCAGATACTTGCGTTCTTCGTCCTTATCGATATTCCCTTTCGACCTTCAGACCTCGCACTTCACTTGTAACTTCCTCTTCTTCTTCTTTTAACATCCCTCTCAATTCGTCTTTGTCCTCTTTTTCTCTGGTTGGGTTACGTCGTGAATCTCCTCTACTGTTATTCGGCGATCCAATTTGGAACGGGAACCCTTTTGTTTGCTCCCAAACCAAACGGAGGGATTTTGGATTGTTAGTTCCTCCTCTTCGATGCGAACCGTCATCGAACGGCAACAGCGTTCAGGGCGAGCGGAGTTTGATGCAATGGATTGAATTGATCGGAGAAGCTTTGTCTACTGCGTTTCCTGTTTGGGTGGCGCTAGGTTGCGTTTTAGGGCTGCTCAGACCGGCTTCCTATGCTTGGGTTCAACCGAGATGGACTGTTCTTGGCATTACTCTCACAATGCTTGGTATGGGCATGACATTGACCCTCGACGATCTTCGTGGAGCCCTGGCTATGCCTAAGGAATTGATTTCTGGGTTCGTGCTCCAGTATTCGGTTAGTTCGTTTGTTTACTGCGATTGTGCTATTGTTTGAACGTATCTGAAATTCATTGATTTTTTTGATCTTGAGAAATTTCTCGCGTATGCTGCATAATTTTGAATGTGTGTGACTTGGGTTTTGGAATATTGGAAGCTGTTCTAGTTCACAGAAAGAGGGAAAGGAACAACAGAAATGACTATGGCCATCAGAAATGTTCCAAATCTCGCAACGGGTGTTGATGGTGAATCATAGCGAGATGAAGAATTACTATTACGAGCTAAATGGATGATTGATTCTGAAGAGTTACTATTACGAGCTAAATGGATGATTGATTCTGAAGAATTACTATTACGAGCTAAATGGATGATTGATTCTGAAGAGTTACTATTACGAGCTAAATGGATGATTGATTCTGAAGAATTACTATTACGAGCTAAATGGATGATTGATTCTTCATCTTTAATATTTCACTACTTTTTTTGAATTGTATGTTAACTGTGTTCCGAAAAGCATTTCGTTTGGATGAAATCCTTCTATTATTGATCTACTGGTTAGATTTTTCTGAGTTTGTACTGAAGGAAACAATTGATAAAAAATAGTGTGACATCGAGGATTTCCACTTCACTTTCTTTGATAGTTTTAGGGACTTCAATCTATAGATGAAATTATAAGATAGACTTTCATGGTTCTTGGTGCAGGTGATGCCAATATCAGGCTTTCTTGTCAGCAAGCTTTTAAATTTGCCATCTTACTATGCAGCTGGTTTAATATTGGTTGGTTGCTGTCCTGGTGGTATGCATCAGTTGGTAGTGTCAATTATCTTTCTCATTCTATTTATTTTAACAGTTTGATCTATCTTTGGTTTCAGGAACAGCAAGCAATATAGTCACTTACATTGCAAGGTATTCTAACTTTTGAAATGCCACCATTTAGCTTCTTTCTTGTTAAATTATGTTAGAGGTTAAGAAAATTTTTATGCCTTTCCAGGGGAAATGTAGCTCTTTCTGTGCTGATGACCGCCACAAGCACTATGGCAGCCGTGGTTAGCAAGCCTCCATAGTGCTTTTGCTTTTTTCGTTTGTTTAGTTTCTAAATTCAAGTTTGCATCTTAGCATGATCTGTTTTTCTTTCGGTTTTATATAGTTCTTCTGTCGTTAAAACAGGACCTAATATCTTTGTTAGTAGAAATGACAACACATAAGAAACCATAGTTTACTCCTAAGTTCATATTTGCACAAACATAGGAAAAACCTTTAACTGAACACAACAATAAATTTTTTATCTACCACGTTACTTTTTTTTTCTCTTCAAAGATATTCGGTTTGATGAGTGTTTACTGTAGAGATGTCAGTGCTATGAGTGAGGAGTCCCTCCTTAATCCGCCTTTTGGGGAGAAAGCCCGGTTTATTTGGCTTGTTGGTGTGTGTGCAGTGTTATGGGTTTTGTGGGATGTGAGGAACAGTGGAGTGTCTGGGGGAGTGGAGAGAAACCCTAGGGAGCTTTGGTCCCTCGTTCGTTTTCATGTTTTTCTCTAGGCTTCAATTTCGTAGATTTTTGGTAATTATTCTATAGGAATGATTTTGCATAGTTGGAGCCCGTTCTTGTAGAGGGCATCTCTCCTTTTTGTGGGCTTTGTTTCCTTAGAACTGTGCATTTTTTCCTTTTTCTCAATGGAAGTGATTATTTTCGAAGTTTCATAGATATTGAAATCTAAGTTATCATTTCAATTTTACAGATCATGACCCCTTTTCTCACTGCCAAACTTGCTGGTCAATTTGTTGCTGTGGACGCAGCTGGGTTGTTAATGTCCACCCTGCAGGTGCGATTTCTAACAAACGAAAGCAACATCAATATTTTTCTTAACATTAAATTTCTCTCAAGTAACTTCCCTTTCCTTCACATACAACAGATTGTGCTTCTTCCAGTGTTGGGTGGGGCATTTTTAAACCAGTATTTCCATGGGTTGGTTAGATTTGTCTCTCCATTCATGCCCCCTATTGCTGTTGGCACTGTTGCTATTTTATGTGGACATGCAATTGCTCAGAGCTCCTCTGCTATTCGGATGTCTGGTCAGCAAGTAGTCCTGGCTGCAGCTCTTCTTCATGCTTCAGGATTTTTCTTCGGTTATGTACTTGCAAGATTGCTTGGCATTGACATTGCATCTTCTCGAACGATCTCCATTGAAGTTGGAATGCAGGTAAAGCATTTCATTTAGAATAATTGCAATGGATAGTACTTTTATGATAAATAATTAAACGCATAGCAATGTGTTAAAAGAATTTTAAATATGGGGAAAATCTATTAATGACAAACATTTATTAGAAATATGGACTATGGTAGACTTTGAGAATTGATTCAAGATTGCATTATATTTGTAAATTGTTTTGTATTGTGCTACATTTATTCATATTTGGGTCTGATCGCTGTTTTTGCAACTATCCCTTTCATTTACTTTCGGGGTATCTTAGTTGCACTCTTTGGCAAGTCTGCTGCCAAGTGTCTTACTACATAACTCAATCAGTTTCGGCATTTTTAGGTTGATCTGGTTTTTAACTTTTAACAATGAATAAATTTATGTGACAGAACTCGGTGCTAGGGGTTGTTCTTGCTAGCCAGCACTTTGGAAATCCTCTTACGGCAGTGCCCTGTGCAGTTTCAAGTGTTTGCCACTCAATCTTTGGCAGCGTTCTTGCTGGGATTTGGAGGCAAAGCAAAGACTAGGTGAAAATGATGTCTAAATTTTGTAAGCATCATTTGTGTTTTATCCTTTTGGTTTAGTAGATTTTTGTAACATTTGAATAGTTCTTGACAGGTTGTTTGCAATCATTCCATGTTTGAACTTTGTCTAATCTTGTTTTAGAATAAATCTTAAATTGTTTTTTTCCAATAAAAATAAATTTAAT

Coding sequence (CDS)

ATGCAGTCTCTTCCATCAGATACTTGCGTTCTTCGTCCTTATCGATATTCCCTTTCGACCTTCAGACCTCGCACTTCACTTGTAACTTCCTCTTCTTCTTCTTTTAACATCCCTCTCAATTCGTCTTTGTCCTCTTTTTCTCTGGTTGGGTTACGTCGTGAATCTCCTCTACTGTTATTCGGCGATCCAATTTGGAACGGGAACCCTTTTGTTTGCTCCCAAACCAAACGGAGGGATTTTGGATTGTTAGTTCCTCCTCTTCGATGCGAACCGTCATCGAACGGCAACAGCGTTCAGGGCGAGCGGAGTTTGATGCAATGGATTGAATTGATCGGAGAAGCTTTGTCTACTGCGTTTCCTGTTTGGGTGGCGCTAGGTTGCGTTTTAGGGCTGCTCAGACCGGCTTCCTATGCTTGGGTTCAACCGAGATGGACTGTTCTTGGCATTACTCTCACAATGCTTGGTATGGGCATGACATTGACCCTCGACGATCTTCGTGGAGCCCTGGCTATGCCTAAGGAATTGATTTCTGGGTTCGTGCTCCAGTATTCGGTGATGCCAATATCAGGCTTTCTTGTCAGCAAGCTTTTAAATTTGCCATCTTACTATGCAGCTGGTTTAATATTGGTTGGTTGCTGTCCTGGTGGTATGCATCAGTTGGTAGTGTCAATTATCTTTCTCATTCTATTTATTTTAACAGTTTGA

Protein sequence

MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLFGDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVSIIFLILFILTV

Homology

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BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 6.4e-51
Identity = 98/157 (62.42%), Postives = 125/157 (79.62%), Query Frame = 0


Query: 69  PFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFPVWVALGCV 128
           P  C ++ R DF   VP  RC  SSN    + ++S  +W+E +GEA+STAFP+WV+LGC+
Sbjct: 57  PISCRRS-RFDF---VP--RCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCL 116

Query: 129 LGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPI 188
           LGL+RP+++ WV P WT++G+T+TMLGMGMTLTLDDLRGAL+MPKEL +GF+LQYSVMP+
Sbjct: 117 LGLMRPSTFNWVTPNWTIVGLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPL 176

Query: 189 SGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVSII 226
           S F VSKLLNLP +YAAGLILVGCCPGG    +V+ I
Sbjct: 177 SAFFVSKLLNLPPHYAAGLILVGCCPGGTASNIVTYI 207

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BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 165.6 bits (418), Expect = 6.6e-40
Identity = 85/153 (55.56%), Postives = 106/153 (69.28%), Query Frame = 0


Query: 84  VPPLRCEPSSN--------GNSVQGERSLMQWIEL---IGEALSTAFPVWVALGCVLGLL 143
           +P  RC  +++        G S   E  ++ W +L   +GE LS  FPVWVA  C + L 
Sbjct: 60  IPSSRCHAAADPAPSKIPGGGSGALEAGVVGWRDLLLQVGEVLSLGFPVWVASACAVALW 119

Query: 144 RPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFL 203
           RP ++ WV P   ++GI+ TMLGMGMTLTLDDL+ AL MPKEL SGF+LQYSVMP+SGFL
Sbjct: 120 RPPAFLWVSPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKELASGFLLQYSVMPLSGFL 179

Query: 204 VSKLLNLPSYYAAGLILVGCCPGGMHQLVVSII 226
           +SKLLNLPSYYAAGLILV CCPGG    +V+ +
Sbjct: 180 ISKLLNLPSYYAAGLILVSCCPGGTASNIVTYL 212

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BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 7.6e-20
Identity = 52/124 (41.94%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 102 RSLMQWIELIGEALSTAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLT 161
           + L Q+ ++I E L+T FP+WV LG ++G+ +P+   W++     LG+   ML MG+TLT
Sbjct: 89  KELSQYEKII-ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLT 148

Query: 162 LDDLRGALAMPKELISGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLV 221
            +D R  L  P  +  GF+ QY + PI GFL++  L L +  A GLILV CCPGG    V
Sbjct: 149 FEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNV 208

Query: 222 VSII 226
            + I
Sbjct: 209 ATYI 211

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BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.2e-19
Identity = 51/138 (36.96%), Postives = 76/138 (55.07%), Query Frame = 0


Query: 88  RCEPSSNGNSVQGERSLMQWIELIGEALSTAFPVWVALGCVLGLLRPASYAWVQPRWTVL 147
           + E + + N  +   S     E I E L+T FPVWV LG ++G+ +P+   W++     +
Sbjct: 83  KAEANISSNLPESIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSMVTWLETDLFTV 142

Query: 148 GITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFLVSKLLNLPSYYAAGL 207
           G+   ML MG+TLT +D R  +  P  +  GF+ QY + P+ GF ++  L L +  A GL
Sbjct: 143 GLGFLMLSMGLTLTFEDFRRCMRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGL 202

Query: 208 ILVGCCPGGMHQLVVSII 226
           ILV CCPGG    V + I
Sbjct: 203 ILVSCCPGGQASNVATYI 220

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BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.3e-14
Identity = 43/121 (35.54%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 105 MQWIELIGEALSTAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDD 164
           M  I  I       F +WV +  VLG   P+ + W+    T+  + + M GMG+TL  DD
Sbjct: 1   MDIIRKISHFAGQTFGIWVIVFAVLGFSFPSLFTWISSYITIF-LGIIMFGMGLTLQADD 60

Query: 165 LRGALAMPKELISGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVSI 224
            +  +  P ++I G + QY++MP+  F ++  L+LP+  A G+ILVGCCPGG    V++ 
Sbjct: 61  FKELVRKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNVMTF 120

Query: 225 I 226
           +
Sbjct: 121 L 120

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BLAST of Cucsat.G8906.T2 vs. NCBI nr
Match: XP_004146013.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis sativus] >KGN55025.1 hypothetical protein Csa_012383 [Cucumis sativus])

HSP 1 Score: 432 bits (1112), Expect = 1.57e-149
Identity = 217/223 (97.31%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 223

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BLAST of Cucsat.G8906.T2 vs. NCBI nr
Match: KAA0066700.1 (putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 417 bits (1073), Expect = 1.33e-143
Identity = 213/224 (95.09%), Postives = 214/224 (95.54%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTF PRTSLVTSSSS  N PLNSSLSSFSLVGLRRES LLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFTPRTSLVTSSSS--NNPLNSSLSSFSLVGLRRESSLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GD IWN NPFVCSQTKR DFGLLVPPLRCEPSSNGNS+QGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVSI 224
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLV  I
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVAKI 222

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BLAST of Cucsat.G8906.T2 vs. NCBI nr
Match: XP_008463794.1 (PREDICTED: LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis melo] >TYK27850.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 407 bits (1046), Expect = 1.57e-139
Identity = 208/223 (93.27%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTF PRTSLVTSSSS  N PLNSSLSSFSLVGLRRES LLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFTPRTSLVTSSSS--NNPLNSSLSSFSLVGLRRESSLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GD IWN NPFVCSQTKR DFGLLVPPLRCEPSSNGNS+QGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 221

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BLAST of Cucsat.G8906.T2 vs. NCBI nr
Match: XP_038898003.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 349 bits (895), Expect = 1.47e-116
Identity = 185/227 (81.50%), Postives = 197/227 (86.78%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPY----RYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESP 60
           MQSLPSDT VLRP+    RYSLS+FR RTSLVTSS+S  N PL SSLSS S  G  RES 
Sbjct: 1   MQSLPSDTFVLRPHIQSHRYSLSSFRRRTSLVTSSTS--NNPLPSSLSSSSPAGFHRESS 60

Query: 61  LLLFGDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALS 120
           L LF + IW+ N F CS+TKR D GLLVPPLRCE SSNGNSVQGERSLMQWIEL+GEALS
Sbjct: 61  LRLFNERIWSRNAFFCSKTKRIDVGLLVPPLRCEASSNGNSVQGERSLMQWIELLGEALS 120

Query: 121 TAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELI 180
           TAFPVWVALGC+LGLLRPASYAWVQP WTVLGITLTMLGMGMTLTL+DLRGALAMPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPASYAWVQPGWTVLGITLTMLGMGMTLTLEDLRGALAMPKELI 180

Query: 181 SGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           SGF+LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 SGFLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 225

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BLAST of Cucsat.G8906.T2 vs. NCBI nr
Match: XP_022982182.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 310 bits (794), Expect = 6.18e-102
Identity = 164/229 (71.62%), Postives = 185/229 (80.79%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRY----SLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESP 60
           M+SLP DTCVLRP+      SLS+F PRTSL+TSS+S      N+  SS S     R + 
Sbjct: 1   MRSLPLDTCVLRPHIQPPPRSLSSFSPRTSLLTSSTS------NNRFSSASAATFHRNAS 60

Query: 61  LLLFGDPI--WNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEA 120
           L +F + I  WN NP +CS+TKR D GLLVPPL+C  SS+ N VQ ERSLMQWIELIGEA
Sbjct: 61  LQIFNERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEA 120

Query: 121 LSTAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKE 180
           LSTAFPVWVALGC+LGLLRPA Y WVQPRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKE
Sbjct: 121 LSTAFPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKE 180

Query: 181 LISGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LISGF+LQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LISGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 223

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BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL
Match: A0A0A0KZF9 (Sodium-bile acid cotransporter OS=Cucumis sativus OX=3659 GN=Csa_4G622840 PE=3 SV=1)

HSP 1 Score: 432 bits (1112), Expect = 7.60e-150
Identity = 217/223 (97.31%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 223

[Close]

BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL
Match: A0A5A7VHN1 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G00330 PE=3 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 6.42e-144
Identity = 213/224 (95.09%), Postives = 214/224 (95.54%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTF PRTSLVTSSSS  N PLNSSLSSFSLVGLRRES LLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFTPRTSLVTSSSS--NNPLNSSLSSFSLVGLRRESSLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GD IWN NPFVCSQTKR DFGLLVPPLRCEPSSNGNS+QGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVSI 224
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLV  I
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVAKI 222

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BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL
Match: A0A5D3DX81 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00270 PE=3 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 7.59e-140
Identity = 208/223 (93.27%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTF PRTSLVTSSSS  N PLNSSLSSFSLVGLRRES LLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFTPRTSLVTSSSS--NNPLNSSLSSFSLVGLRRESSLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GD IWN NPFVCSQTKR DFGLLVPPLRCEPSSNGNS+QGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 221

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BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL
Match: A0A1S3CK43 (LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501847 PE=3 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 7.59e-140
Identity = 208/223 (93.27%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESPLLLF 60
           MQSLPSDTCVLRPYRYSLSTF PRTSLVTSSSS  N PLNSSLSSFSLVGLRRES LLLF
Sbjct: 1   MQSLPSDTCVLRPYRYSLSTFTPRTSLVTSSSS--NNPLNSSLSSFSLVGLRRESSLLLF 60

Query: 61  GDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALSTAFP 120
           GD IWN NPFVCSQTKR DFGLLVPPLRCEPSSNGNS+QGERSLMQWIELIGEALSTAFP
Sbjct: 61  GDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFP 120

Query: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180
           VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV
Sbjct: 121 VWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFV 180

Query: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 221

[Close]

BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL
Match: A0A6J1IVZ2 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481085 PE=3 SV=1)

HSP 1 Score: 310 bits (794), Expect = 2.99e-102
Identity = 164/229 (71.62%), Postives = 185/229 (80.79%), Query Frame = 0


Query: 1   MQSLPSDTCVLRPYRY----SLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESP 60
           M+SLP DTCVLRP+      SLS+F PRTSL+TSS+S      N+  SS S     R + 
Sbjct: 1   MRSLPLDTCVLRPHIQPPPRSLSSFSPRTSLLTSSTS------NNRFSSASAATFHRNAS 60

Query: 61  LLLFGDPI--WNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEA 120
           L +F + I  WN NP +CS+TKR D GLLVPPL+C  SS+ N VQ ERSLMQWIELIGEA
Sbjct: 61  LQIFNERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEA 120

Query: 121 LSTAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKE 180
           LSTAFPVWVALGC+LGLLRPA Y WVQPRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKE
Sbjct: 121 LSTAFPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKE 180

Query: 181 LISGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHQLVVS 223
           LISGF+LQYSVMPISG++VSKLLNLPSYYAAGLILVGCCPGG    +V+
Sbjct: 181 LISGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVT 223

The following BLAST results are available for this feature:

BLAST of Cucsat.G8906.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93YR2	6.4e-51	62.42	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis tha...	[more]
Q7XVB3	6.6e-40	55.56	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa su...	[more]
Q1EBV7	7.6e-20	41.94	Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
Q5VRB2	2.2e-19	36.96	Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su...	[more]
O34524	1.3e-14	35.54	Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 1...	[more]

BLAST of Cucsat.G8906.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146013.1	1.57e-149	97.31	probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis sativus] ...	[more]
KAA0066700.1	1.33e-143	95.09	putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa]	[more]
XP_008463794.1	1.57e-139	93.27	PREDICTED: LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, ...	[more]
XP_038898003.1	1.47e-116	81.50	probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida...	[more]
XP_022982182.1	6.18e-102	71.62	probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 [Cucurb...	[more]

BLAST of Cucsat.G8906.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZF9	7.60e-150	97.31	Sodium-bile acid cotransporter OS=Cucumis sativus OX=3659 GN=Csa_4G622840 PE=3 S...	[more]
A0A5A7VHN1	6.42e-144	95.09	Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3DX81	7.59e-140	93.27	Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CK43	7.59e-140	93.27	LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, chloroplast...	[more]
A0A6J1IVZ2	2.99e-102	71.62	probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 OS=Cucu...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038770	Sodium/solute symporter superfamily	GENE3D	1.20.1530.20		coord: 102..228 e-value: 4.2E-26 score: 93.7
IPR002657	Bile acid:sodium symporter/arsenical resistance protein Acr3	PFAM	PF01758	SBF	coord: 147..222 e-value: 7.6E-16 score: 58.4
None	No IPR available	PANTHER	PTHR10361:SF64	SODIUM/METABOLITE COTRANSPORTER BASS1, CHLOROPLASTIC-RELATED	coord: 85..223
IPR004710	Bile acid:sodium symporter	PANTHER	PTHR10361	SODIUM-BILE ACID COTRANSPORTER	coord: 85..223

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8906	Cucsat.G8906	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8906.T2.E1	Cucsat.G8906.T2.E1	exon
Cucsat.G8906.T2.E2	Cucsat.G8906.T2.E2	exon
Cucsat.G8906.T2.E3	Cucsat.G8906.T2.E3	exon
Cucsat.G8906.T2.E4	Cucsat.G8906.T2.E4	exon
Cucsat.G8906.T2.E5	Cucsat.G8906.T2.E5	exon
Cucsat.G8906.T2.E6	Cucsat.G8906.T2.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8906.T2.C2	Cucsat.G8906.T2.C2	CDS
Cucsat.G8906.T2.C1	Cucsat.G8906.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8906.T2	Cucsat.G8906.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098717	pantothenate import across plasma membrane
cellular_component	GO:0016020	membrane
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane