Cucsat.G8875.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8875.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome b561 domain-containing protein
Location	ctg1635: 968667 .. 972044 (-)
RNA-Seq Expression	Cucsat.G8875.T1
Synteny	Cucsat.G8875.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTTTTAGGTGAATCGATTAGATAGAGAGATGAGTAGTTGTATTTGTTGAGAAAAATCAAGTACCATAGTCCAGTGTCCTCTTTTGGGCATTATTGATCACTACCTTTAGTGGTCCTCCTCTGACATTGTTCTACTTTTTCCTTTTTCCTTATTGCTACAAAAGCTTTCAAGATCCCTTGTGAAATCCATAGCAACAACCTACAAGGGTGGCTTCACCATTCTGCTTTGTGCTTCTTTCATCAGAACCAAATAAACAATGCTATTTTTGCAGAAGACGGCTTTTACTTTATCCATCCCTCCAAGTTCAATACTTTTTATCTTTCTTCTTCTCACATTTCAACAAGTCAGCTCATCTCAACAGCACAACAAAAACGAAGACATGATTCATTCAAGCAAGAAAGACAACAGCCAAAAGGTTTCCATTTCTTCTTTCAGTTCATATTTTACATGTAATGAGAAAAGTGGTTTGTAATGTAATTAAAGCTGAAATTGATTACTGGTTATATAATGGCAACAGATGAGTTCCAGCCTATTGTTTGATATCACACTCCATGGATTTCTCCTATGGGCTTCAATGGGATTCCTTATGCCTGTTGGGATTCTTGTTATCAGAATGTCTAACAGAGAGCAATGTGGAAGAAAGCTGAAATATTACTTTTACATTCATACTATTCTACAGGCAAGTTCATCTAATCTTTTCTAGCAATATCATAATAGAATGAAAAACTGAGCCGGCCGAACAAAACTAATCGAACTGAGGAGAGGAGAGGTTAGTGTCAGTTTAAACAATTTTAAAAAATATTCAAAGACTTAAATCAACCGATCTTTTTTGTACTCGTTCATTACAGGAATCGGTTTGAACATTTAACTAAGTCGATCACAGTCGATTCAGTGTCGGTTTAGTCGGAAAACCAAACCCAACAAACCGATACTCACCCCTAAACAATACACTCAAATACTAAAATGAACGTTCTTTTTTGTTTTTGTTATTGTTTACATAAAGAAGAATCCTTTATAACTAAACAAACAATAAATGTAGAAGCATCATTAAGGCAATTAACTTTAACTTTTTATTAAACAAGCAAACAATCCACTTTTTCTTGCTCTACTTTATGAAATTAATTATAGTTAAAGAGAAACTATAAGGGTCCTGATTCTGAATATCTTATACATGAATATACTTGTGAAGAAAGAAAAGACAGGCATCATCACATCATAACTAATTAGAAACTCATAGCCAAATATCTGTAATGTTGAAAATCTAGCAGGTGTATGGATTATAATATTAGAGTTAGGTGAGATTTAGCTCTGGAAAGTCAAAATGATACAAAATTTGTATGACAGTTGAAGGATTTTGATTATACTTCAACCACAAAGATGGGGTCCAATTTGATGAATCTCACTTCAAGATCAAACAGTCGTGGTTCAAGTGATTATAATGCTAGTATTTTTCATTCTCACAATGAGTTGTTAAATCAGCCATTAATATCATATTATCTTGATTTGATTATGTTGAACAGATAGTGTCTGTATTACTAGTCACAGCAGGAGCAGTCATGTCTATCAAGAAATTCAACAATGCTTTCAATAATAATCACCAAAGGATAGGGATTGGCTTGTATGGGATGATCTGGTTACAGGGGTTGATTGGAATTGTAAGGCCAAAAAGGTAAACTTTCATATTTCATATGATTATAAAGCCTCGCTAGTTTCTTGGAAAGAGTCATTAAAGTCCCGTTTGATAACAATTTTATTTTTTAAAATTAAGTTTCACACTATCTTGTTCTATATTCAAAACCATTCTAAGACTAAGAGAGCGCTTGAGTTATAATGATATGTGTTTGGACTATAAACTATTTTAGTATGATCAGAAGTAAATAATAAATATTGTAGCAAAGAATAATAAAAGATGAATGCCAAATAGTAATAACAAATAAAACGCTTTGAAATAGTGTTTATTGTGAAATTTTTAAATAGGATTTAATGTATAATAGTCTTAGTCTTACTTAGTTCTGACTCGAAACACCCTTAAATGTTTGTTCGAGCCTAATTTTGGATTGATGAAGAATATTGGTTGTTGTAAAAATTGGTCAATGCATACAAGAAAAGACTTGAACAAAAACAACTTTTGCGAACCCTATTCATCAATCATGTCTCTAATAAATCTTTTGTTAAAAAATACTCTGCATATCAACTTATACCCCTAAACTTTAGATTTTTTTCAATTTAAACTATGATTTTTCATTAATTTACCCTTTCCAATTTTTGTTTAACCAATACTACGTTAAACTTATAATTCGAGTCAATTAAACTCCAAAATTTCAATAAGCCAATCAACTTAGACCCTTCGTTATGATTACCTTTGAAAATCATCAATATATTGATGCACAAATACATCTAAGCTTCTCTTCAAATGTAAAATTTTACAAAATTAGATTATTAGAGTGAATGTATGGACACAATTCTCCAATAAAAAAAATATAATTAGGAGTCTATTGATTCATTAACGTATGTCTAGGAGTTTACACACCAAAATGTAATAGAGAGGATACACTTGCAAAACTTCAAAGTTGGAATTAATTCAACCTCTAAGGTTGAGGGGTACAGATGAACATTTCAAAACCAAACAAACAAACGAGCAAAGTTTTCTTACTCCAAAATCAACTGCAGATTATCTAGATGGAGTAAACAGTCAGGACGATATTGATATCGACTTCTAAACTCTAAATAAACTCTTCATTGACTGTTGCAGGGGGTCTAAAACAAGAAGCGTATGGTTTTTCATACATTGGATGCTTGGGACAGCAGTATCTCTGCTAGGAGTTTTCAATGTATACTCAGGATTATTTGCATACCATGAGAAAACATCTCAAAGTATAAGAATTTGGACTATAATTTTTAGTGTTGAAATTTCTTTGATATCATTCTTTTACCTATTCCAAGACAAAGTAGAGTATATACAAAAGCAAGGAGTTACTTTGGGGAATGATTTGGTAGTACTGTCTCCAAATGACAAGCAAAAGGAACTTCCTACTGATCCATGCTGAAGAATTAAGGAGTAAATAAAATAGAGTTGTCAAAACGTTGCTTCTTTCTTTCTTTTTTTTTTTCTCTCTTTTCACTAACATGAGATTGGTGTTATTCTTCTTACTCATTTCTCTCTTCTTCTTGGGATTTGTATAGGAAAAGAGTGTGGTCTTTGTAAGAAATGGAAAATCTTTTGGGTTTGGAGGAGATGTTTTTTTGATAAGACAATGTGAGTTTTTTTCTGTTTTCTATTACAGATTTTCACATAATGGATACAAAGTTTTCTTTTTTTGAAGAATAAAGATGTGAGCACAGATACTGATATTGCTCTTTTAATAATATCACAAGA

Coding sequence (CDS)

ATGCTATTTTTGCAGAAGACGGCTTTTACTTTATCCATCCCTCCAAGTTCAATACTTTTTATCTTTCTTCTTCTCACATTTCAACAAGTCAGCTCATCTCAACAGCACAACAAAAACGAAGACATGATTCATTCAAGCAAGAAAGACAACAGCCAAAAGATGAGTTCCAGCCTATTGTTTGATATCACACTCCATGGATTTCTCCTATGGGCTTCAATGGGATTCCTTATGCCTGTTGGGATTCTTGTTATCAGAATGTCTAACAGAGAGCAATGTGGAAGAAAGCTGAAATATTACTTTTACATTCATACTATTCTACAGATAGTGTCTGTATTACTAGTCACAGCAGGAGCAGTCATGTCTATCAAGAAATTCAACAATGCTTTCAATAATAATCACCAAAGGATAGGGATTGGCTTGTATGGGATGATCTGGTTACAGGGGTTGATTGGAATTGTAAGGCCAAAAAGGGGGTCTAAAACAAGAAGCGTATGGTTTTTCATACATTGGATGCTTGGGACAGCAGTATCTCTGCTAGGAGTTTTCAATGTATACTCAGGATTATTTGCATACCATGAGAAAACATCTCAAAGTATAAGAATTTGGACTATAATTTTTAGTGTTGAAATTTCTTTGATATCATTCTTTTACCTATTCCAAGACAAAGTAGAGTATATACAAAAGCAAGGAGTTACTTTGGGGAATGATTTGGTAGTACTGTCTCCAAATGACAAGCAAAAGGAACTTCCTACTGATCCATGCTGA

Protein sequence

MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVMSIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLGVFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVLSPNDKQKELPTDPC

Homology

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy Swiss-Prot
Match: Q0WPS2 (Cytochrome b561 domain-containing protein At4g18260 OS=Arabidopsis thaliana OX=3702 GN=At4g18260 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.7e-52
Identity = 114/264 (43.18%), Postives = 166/264 (62.88%), Query Frame = 0


Query: 9   FTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLFDITLHGFL 68
           F L+  P  ++   L +T    S S    K      S ++D   K+S  ++  I LHG L
Sbjct: 16  FYLTSAPPFVICSSLEVTIDNHSPSNLKTKG-----SLEQD---KLSHQMINSIKLHGIL 75

Query: 69  LWASMGFLMPVGILVIRMSNR-EQCGRKLKYYFYIHTILQIVSVLLVTAGAVMSIKKFNN 128
           LW SMGFLMPVGIL IRM+N+  + G K+K +FY+H I QI++V+L T GA++S++   N
Sbjct: 76  LWVSMGFLMPVGILFIRMANKAHENGIKVKVFFYLHVIFQILAVVLATIGAILSLRTLEN 135

Query: 129 AFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLGVFNVYSG 188
           +F+NNHQR+G+ LY  +WLQ L G+ +P RGSK R  WF +HW+LGT VS++G+ N+Y+G
Sbjct: 136 SFDNNHQRLGLALYAAMWLQFLTGVFKPSRGSKRRLRWFLLHWILGTIVSIVGIVNIYTG 195

Query: 189 LFAYHEKTSQS--IRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTL------------ 248
           + AY +KTS S    +WTI+F+V+++ + FFYL+QDK E+ QKQ V L            
Sbjct: 196 IQAYQKKTSLSRDSSLWTILFTVQVTCLVFFYLYQDKWEHFQKQRVVLDELDHQNNNTNG 255

Query: 249 --GNDLVVLSPND-KQKELPTDPC 255
                + V++ ND +QK +   PC
Sbjct: 256 RNNQSIQVVTRNDHEQKVMVPQPC 271

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy Swiss-Prot
Match: O80854 (Cytochrome b561 domain-containing protein At2g30890 OS=Arabidopsis thaliana OX=3702 GN=At2g30890 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-50
Identity = 100/224 (44.64%), Postives = 154/224 (68.75%), Query Frame = 0


Query: 19  LFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDN---SQKMSSSLLFDITLHGFLLWASMGF 78
           L + LL     + SSQ++ ++  +  +   +    S+K++  L+++I +HGF+LWA+MG 
Sbjct: 7   LLVSLLFLLLPLCSSQENTRSLAIDVNGPVETSPISEKLNPKLVYEIKVHGFMLWAAMGV 66

Query: 79  LMPVGILVIR-MSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVMSIKKFNNAFNNNHQ 138
           LMP+GI+ IR MS ++Q    L+  F++H   Q+V+V+LVT GAVMS+  FNN+F+N+HQ
Sbjct: 67  LMPIGIISIRLMSIKDQPIITLRRLFFLHVTSQMVAVILVTIGAVMSVINFNNSFSNHHQ 126

Query: 139 RIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLGVFNVYSGLFAYHEK 198
           ++GIGLY ++W Q L+G +RP R  K R  WF  HW+LGT++++LG+ N+Y+GL AY +K
Sbjct: 127 QLGIGLYVIVWFQALLGFLRPPREEKARRKWFVGHWILGTSIAILGIINIYTGLHAYAKK 186

Query: 199 TSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV 239
           TS+S  +WTI+F+ ++S I+  YLFQDK  YIQ Q     N  V
Sbjct: 187 TSKSANLWTILFTAQLSCIALVYLFQDKWSYIQSQATFNRNQSV 230

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy Swiss-Prot
Match: Q9SV71 (Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis thaliana OX=3702 GN=At4g12980 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.5e-10
Identity = 39/134 (29.10%), Postives = 66/134 (49.25%), Query Frame = 0


Query: 64  LHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVMSIK 123
           +HG L   S G L P+G ++ R     +       +FY+H   Q  + ++  AG    +K
Sbjct: 220 IHGILNAVSWGLLFPIGAMIARYMRIFESADPA--WFYLHVSCQFSAYVIGVAGWATGLK 279

Query: 124 KFNNAFN---NNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 183
             + +     N H+ IGI L+ +  LQ    ++RP++  K R VW   H  +G ++ +LG
Sbjct: 280 LGSESKGIQYNTHRNIGICLFSIATLQMFAMLLRPRKDHKFRFVWNIYHHGVGYSILILG 339

Query: 184 VFNVYSGLFAYHEK 195
           + NV+ GL   + K
Sbjct: 340 IINVFKGLSILNPK 351

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy Swiss-Prot
Match: Q8VYH6 (Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis thaliana OX=3702 GN=At4g17280 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 41/136 (30.15%), Postives = 64/136 (47.06%), Query Frame = 0


Query: 56  SSLLFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVT 115
           SS L    +HG L   S G +MP+G ++ R     +       +FYIH   Q  + ++  
Sbjct: 210 SSKLRKRNIHGILNGVSWGIMMPLGAIIARYLRVAKSADPA--WFYIHVFCQASAYIIGV 269

Query: 116 AGAVMSIKKFNNAFN---NNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWML 175
           AG    +K   ++     + H+ IGI L+ +  +Q     +RPK   K R  W   H  +
Sbjct: 270 AGWATGLKLGGDSPGIQYSTHRAIGIALFSLATVQVFAMFLRPKPEHKHRLYWNIYHHTI 329

Query: 176 GTAVSLLGVFNVYSGL 189
           G  + +LGV NV+ GL
Sbjct: 330 GYTIIILGVVNVFKGL 343

[Close]

BLAST of Cucsat.G8875.T1 vs. NCBI nr
Match: XP_004144633.1 (cytochrome b561 domain-containing protein At4g18260 [Cucumis sativus] >KGN54933.1 hypothetical protein Csa_012051 [Cucumis sativus])

HSP 1 Score: 501 bits (1290), Expect = 9.29e-179
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF
Sbjct: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240
           VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL
Sbjct: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240

Query: 241 SPNDKQKELPTDPC 254
           SPNDKQKELPTDPC
Sbjct: 241 SPNDKQKELPTDPC 254

[Close]

BLAST of Cucsat.G8875.T1 vs. NCBI nr
Match: XP_008465444.1 (PREDICTED: cytochrome b561 domain-containing protein At4g18260-like [Cucumis melo])

HSP 1 Score: 481 bits (1237), Expect = 1.12e-170
Identity = 242/254 (95.28%), Postives = 249/254 (98.03%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK AF LSIP SSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF
Sbjct: 1   MLFLQKKAFALSIPASSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMP+GILVIRMSNREQCGRKLKYYFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPIGILVIRMSNREQCGRKLKYYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQGLIGIVRPKRGSKTRS+WFFIHWM+GTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSLWFFIHWMVGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240
           VFNVYSGLFAYHEKTS+SIRIWTIIFSVEI LISFFYLFQDKVEYIQKQGVTLGN+LVVL
Sbjct: 181 VFNVYSGLFAYHEKTSESIRIWTIIFSVEICLISFFYLFQDKVEYIQKQGVTLGNELVVL 240

Query: 241 SPNDKQKELPTDPC 254
           SPN KQKELPTDPC
Sbjct: 241 SPNHKQKELPTDPC 254

[Close]

BLAST of Cucsat.G8875.T1 vs. NCBI nr
Match: XP_038897321.1 (cytochrome b561 domain-containing protein At4g18260-like [Benincasa hispida] >XP_038897322.1 cytochrome b561 domain-containing protein At4g18260-like [Benincasa hispida])

HSP 1 Score: 447 bits (1149), Expect = 3.33e-157
Identity = 229/259 (88.42%), Postives = 242/259 (93.44%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK AFT SIP SSILF+ LLLTFQ VSSSQQ NKNED   +SKKDNSQ+MSSSLLF
Sbjct: 1   MLFLQKKAFTFSIPASSILFLLLLLTFQPVSSSQQPNKNEDF-PTSKKDNSQQMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGIL+IRMSNREQCGR+LK+YFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILIIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQ LIGIVRPKRGSKTRS+WFF+HWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSKTRSLWFFVHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV-- 240
           +FNVYSGLFAYHEKTSQSIRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LGNDLV  
Sbjct: 181 IFNVYSGLFAYHEKTSQSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLVIP 240

Query: 241 ---VLSPNDKQKELPTDPC 254
              VL PNDKQKELPTDPC
Sbjct: 241 TDQVLFPNDKQKELPTDPC 258

[Close]

BLAST of Cucsat.G8875.T1 vs. NCBI nr
Match: XP_022936959.1 (cytochrome b561 domain-containing protein At4g18260-like [Cucurbita moschata])

HSP 1 Score: 436 bits (1120), Expect = 8.74e-153
Identity = 226/259 (87.26%), Postives = 239/259 (92.28%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK  F  SIP SSILF+ LLLTFQ VSSSQQ  KNE+  H+SKKD+SQKMSSSLLF
Sbjct: 1   MLFLQKRMFPFSIPASSILFLLLLLTFQPVSSSQQPYKNENF-HTSKKDHSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMS+REQCGR+LK+YFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSSREQCGRRLKFYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQ LIGIVRPKRGS TRS+WFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSNTRSLWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV-- 240
           VFNVYSGLFAYHEKTS+SIRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LGNDLV  
Sbjct: 181 VFNVYSGLFAYHEKTSRSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLVTP 240

Query: 241 ---VLSPNDKQKELPTDPC 254
              VLSPNDKQKEL TDPC
Sbjct: 241 TDQVLSPNDKQKELNTDPC 258

[Close]

BLAST of Cucsat.G8875.T1 vs. NCBI nr
Match: XP_023535975.1 (cytochrome b561 domain-containing protein At4g18260-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434 bits (1116), Expect = 3.56e-152
Identity = 225/259 (86.87%), Postives = 238/259 (91.89%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK  F  SIP SSILF+ LLL FQ VSSSQQ  KNE+  H+SKKD+SQKMSSSLLF
Sbjct: 1   MLFLQKRMFPFSIPASSILFLLLLLKFQSVSSSQQPYKNENF-HTSKKDHSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMS+REQCGR+LK+YFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSSREQCGRRLKFYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQ LIGIVRPKRGS TRS+WFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSNTRSLWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV-- 240
           VFNVYSGLFAYHEKTS+SIRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LGNDLV  
Sbjct: 181 VFNVYSGLFAYHEKTSRSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLVTP 240

Query: 241 ---VLSPNDKQKELPTDPC 254
              VLSPNDKQKEL TDPC
Sbjct: 241 TDQVLSPNDKQKELTTDPC 258

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL
Match: A0A0A0KZN5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G608090 PE=4 SV=1)

HSP 1 Score: 501 bits (1290), Expect = 4.50e-179
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF
Sbjct: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240
           VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL
Sbjct: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240

Query: 241 SPNDKQKELPTDPC 254
           SPNDKQKELPTDPC
Sbjct: 241 SPNDKQKELPTDPC 254

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL
Match: A0A1S3CNW7 (cytochrome b561 domain-containing protein At4g18260-like OS=Cucumis melo OX=3656 GN=LOC103503052 PE=4 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 5.41e-171
Identity = 242/254 (95.28%), Postives = 249/254 (98.03%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK AF LSIP SSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF
Sbjct: 1   MLFLQKKAFALSIPASSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMP+GILVIRMSNREQCGRKLKYYFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPIGILVIRMSNREQCGRKLKYYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQGLIGIVRPKRGSKTRS+WFFIHWM+GTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSLWFFIHWMVGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLVVL 240
           VFNVYSGLFAYHEKTS+SIRIWTIIFSVEI LISFFYLFQDKVEYIQKQGVTLGN+LVVL
Sbjct: 181 VFNVYSGLFAYHEKTSESIRIWTIIFSVEICLISFFYLFQDKVEYIQKQGVTLGNELVVL 240

Query: 241 SPNDKQKELPTDPC 254
           SPN KQKELPTDPC
Sbjct: 241 SPNHKQKELPTDPC 254

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL
Match: A0A6J1F9S9 (cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita moschata OX=3662 GN=LOC111443391 PE=4 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 4.23e-153
Identity = 226/259 (87.26%), Postives = 239/259 (92.28%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK  F  SIP SSILF+ LLLTFQ VSSSQQ  KNE+  H+SKKD+SQKMSSSLLF
Sbjct: 1   MLFLQKRMFPFSIPASSILFLLLLLTFQPVSSSQQPYKNENF-HTSKKDHSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMS+REQCGR+LK+YFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSSREQCGRRLKFYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQ LIGIVRPKRGS TRS+WFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSNTRSLWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV-- 240
           VFNVYSGLFAYHEKTS+SIRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LGNDLV  
Sbjct: 181 VFNVYSGLFAYHEKTSRSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLVTP 240

Query: 241 ---VLSPNDKQKELPTDPC 254
              VLSPNDKQKEL TDPC
Sbjct: 241 TDQVLSPNDKQKELNTDPC 258

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL
Match: A0A6J1II03 (cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita maxima OX=3661 GN=LOC111477093 PE=4 SV=1)

HSP 1 Score: 433 bits (1113), Expect = 4.93e-152
Identity = 224/259 (86.49%), Postives = 239/259 (92.28%), Query Frame = 0


Query: 1   MLFLQKTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSLLF 60
           MLFLQK  F  SIP SSILF+ LLLTFQ VSSSQQ  KNE+  ++SKKD+SQKMSSSLLF
Sbjct: 1   MLFLQKRMFPFSIPASSILFLLLLLTFQPVSSSQQPYKNENF-NTSKKDHSQKMSSSLLF 60

Query: 61  DITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGAVM 120
           DITLHGFLLWASMGFLMPVGILVIRMS+REQCGR+LK+YFYIHTILQI+SVLLVTAGAVM
Sbjct: 61  DITLHGFLLWASMGFLMPVGILVIRMSSREQCGRRLKFYFYIHTILQILSVLLVTAGAVM 120

Query: 121 SIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSLLG 180
           SIKKFNNAFNN+HQRIGIGLYGMIWLQ LIGIVRPKRGS TRS+WFFIHWMLGTAVSLLG
Sbjct: 121 SIKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSNTRSLWFFIHWMLGTAVSLLG 180

Query: 181 VFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV-- 240
           VFNVYSGLFAYHEKTS+SIRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LGNDL+  
Sbjct: 181 VFNVYSGLFAYHEKTSRSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLITP 240

Query: 241 ---VLSPNDKQKELPTDPC 254
              VLSPNDKQKEL TDPC
Sbjct: 241 TDQVLSPNDKQKELTTDPC 258

[Close]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL
Match: A0A6J1FI32 (cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445961 PE=4 SV=1)

HSP 1 Score: 415 bits (1066), Expect = 5.96e-145
Identity = 218/261 (83.52%), Postives = 236/261 (90.42%), Query Frame = 0


Query: 1   MLFLQ--KTAFTLSIPPSSILFIFLLLTFQQVSSSQQHNKNEDMIHSSKKDNSQKMSSSL 60
           MLFLQ  K++F       SILF+ LLLTFQ +SSSQQ  KN +  ++SKKD+SQK+SSSL
Sbjct: 1   MLFLQNKKSSF-------SILFLLLLLTFQPLSSSQQLKKNVEF-NTSKKDHSQKISSSL 60

Query: 61  LFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGRKLKYYFYIHTILQIVSVLLVTAGA 120
           LFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGR+LK+YFYIHTILQI+SVLLVTAGA
Sbjct: 61  LFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGA 120

Query: 121 VMSIKKFNNAFNNNHQRIGIGLYGMIWLQGLIGIVRPKRGSKTRSVWFFIHWMLGTAVSL 180
           VMSIKKFNNAFNNNHQRIGIGLYGMIWLQ LIGIVRPKRGS+TRS+WFFIHWMLGTAVSL
Sbjct: 121 VMSIKKFNNAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSL 180

Query: 181 LGVFNVYSGLFAYHEKTSQSIRIWTIIFSVEISLISFFYLFQDKVEYIQKQGVTLGNDLV 240
           LGVFNVYSGLFAYHEKTS+ IRIWTIIF+VEISLISFFYLFQDK+EYIQKQGV LG+DLV
Sbjct: 181 LGVFNVYSGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLV 240

Query: 241 V-----LSPNDKQKELPTDPC 254
                 LSPNDKQKELPTDPC
Sbjct: 241 TPTDQGLSPNDKQKELPTDPC 253

The following BLAST results are available for this feature:

BLAST of Cucsat.G8875.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WPS2	1.7e-52	43.18	Cytochrome b561 domain-containing protein At4g18260 OS=Arabidopsis thaliana OX=3...	[more]
O80854	1.2e-50	44.64	Cytochrome b561 domain-containing protein At2g30890 OS=Arabidopsis thaliana OX=3...	[more]
Q9SV71	3.5e-10	29.10	Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis tha...	[more]
Q8VYH6	1.7e-09	30.15	Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis tha...	[more]

BLAST of Cucsat.G8875.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144633.1	9.29e-179	100.00	cytochrome b561 domain-containing protein At4g18260 [Cucumis sativus] >KGN54933....	[more]
XP_008465444.1	1.12e-170	95.28	PREDICTED: cytochrome b561 domain-containing protein At4g18260-like [Cucumis mel...	[more]
XP_038897321.1	3.33e-157	88.42	cytochrome b561 domain-containing protein At4g18260-like [Benincasa hispida] >XP...	[more]
XP_022936959.1	8.74e-153	87.26	cytochrome b561 domain-containing protein At4g18260-like [Cucurbita moschata]	[more]
XP_023535975.1	3.56e-152	86.87	cytochrome b561 domain-containing protein At4g18260-like [Cucurbita pepo subsp. ...	[more]

BLAST of Cucsat.G8875.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZN5	4.50e-179	100.00	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G60...	[more]
A0A1S3CNW7	5.41e-171	95.28	cytochrome b561 domain-containing protein At4g18260-like OS=Cucumis melo OX=3656...	[more]
A0A6J1F9S9	4.23e-153	87.26	cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita moschata O...	[more]
A0A6J1II03	4.93e-152	86.49	cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita maxima OX=...	[more]
A0A6J1FI32	5.96e-145	83.52	cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 64..188 e-value: 1.4E-31 score: 120.9
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 64..188 e-value: 1.2E-10 score: 41.7
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 27..227 score: 23.056677
None	No IPR available	GENE3D	1.20.120.1770		coord: 8..225 e-value: 1.7E-23 score: 84.9
None	No IPR available	PANTHER	PTHR15422:SF42	EUKARYOTIC CYTOCHROME B561 PROTEIN	coord: 16..248
None	No IPR available	CDD	cd08760	Cyt_b561_FRRS1_like	coord: 62..221 e-value: 2.54461E-34 score: 120.142
IPR045150	Transmembrane reductase CYB561D1/2	PANTHER	PTHR15422	OS05G0565100 PROTEIN	coord: 16..248

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8875	Cucsat.G8875	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8875.T1.E1	Cucsat.G8875.T1.E1	exon
Cucsat.G8875.T1.E2	Cucsat.G8875.T1.E2	exon
Cucsat.G8875.T1.E3	Cucsat.G8875.T1.E3	exon
Cucsat.G8875.T1.E4	Cucsat.G8875.T1.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8875.T1.C4	Cucsat.G8875.T1.C4	CDS
Cucsat.G8875.T1.C3	Cucsat.G8875.T1.C3	CDS
Cucsat.G8875.T1.C2	Cucsat.G8875.T1.C2	CDS
Cucsat.G8875.T1.C1	Cucsat.G8875.T1.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8875.T1	Cucsat.G8875.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0140575	transmembrane monodehydroascorbate reductase activity
molecular_function	GO:0020037	heme binding