Cucsat.G8435.T8 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8435.T8
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Phloem lectin
Location	ctg1557: 2146353 .. 2147328 (-)
RNA-Seq Expression	Cucsat.G8435.T8
Synteny	Cucsat.G8435.T8

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCATCCATATCTCTACTCTCTTACTATCCAAATTAACCTCTTGAAATGGTTTCCTCACAGCTTGAAGCAAGAGAGAACTCGGAAACAAAAGAGAAATATGGTCATTGCTTGAGTTATATTTTGCCAACAACTGGAGATCATGAGGTGCACTGGCCCTCCCCCTCTGAGCTCTATAAACAACTTTGTGATGGGACTTCCCTCAACAATGGAACTAAGGCAAGTGTAACATTATTTTTAATTTCTTTTAGAGTATTATATAGTTGATATTAGAAGGACTTATAAAAGAGTTAAAAGATATGATTATGAGTGATTTTGTAAAGACTAAAATCACTCATTTCAACTTGTCTCAAATCACTTTTCTTCTAAAATTGTGTTTGTTCTATTTCTAAATTTCTTCTTTACATCTAATAATCTTAACTTCCATGCATATGCATGTAATTATTTTTAATCTAAATTCCTTTTAGTAATTGGATGATTCTACAACTAACATTAAAAACAAAAAAACATTAGTCCTTGGAAAAATTATTTCAATTGATCATACATGGAACAAAACTATTTCTACAAGTATCAAACCAAATATTTACTAGAAAAAAAAAATACTTAGTTAAAAGTATAGAACCAAATATTAAAATGATTCTCGAACAAATCTTGATATTAGCGTTCAATTTTTTTACATAGAAATAAACTTAAAACACAAAAGATATGGAAATTTCTTTTTTGTTATTATTATTATTATTTATGTTTAATTAAAATTTGTTAAAGAGTTGAGTATGATTAGTTTGTATGCAGATTTATTGGTTTCATAGGAAGGGAAGGGGTAATGCCTACTTCATATTTCCAAAAGCCATCTCAATAGCTTGGATCAATGAATGTCAGTACTGGAGATGGATATCTATCGAGATCTTTGGGTATACTTATTTTTTCTAACCGCATTAAATATCGTCTTCGTTTCTATATTTTGAAATATCAATTTT

Coding sequence (CDS)

ATGGTTTCCTCACAGCTTGAAGCAAGAGAGAACTCGGAAACAAAAGAGAAATATGGTCATTGCTTGAGTTATATTTTGCCAACAACTGGAGATCATGAGGTGCACTGGCCCTCCCCCTCTGAGCTCTATAAACAACTTTGTGATGGGACTTCCCTCAACAATGGAACTAAGATTTATTGGTTTCATAGGAAGGGAAGGGGTAATGCCTACTTCATATTTCCAAAAGCCATCTCAATAGCTTGGATCAATGAATGTCAGTACTGGAGATGGATATCTATCGAGATCTTTGGGTATACTTATTTTTTCTAA

Protein sequence

MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYWFHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFGYTYFF

Homology

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy Swiss-Prot
Match: Q9ZVR5 (Putative F-box protein PP2-B2 OS=Arabidopsis thaliana OX=3702 GN=PP2B2 PE=4 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 5.1e-05
Identity = 22/58 (37.93%), Postives = 33/58 (56.90%), Query Frame = 0


Query: 38  SPSELYKQLCDGTSL-NNGTKIYWFHRKGRGNAYFIFP-KAISIAWINECQYWRWISI 94
           S  ELY  +CD   L  +G K +W  ++     + + P K++ I W++  QYWRWISI
Sbjct: 107 SKKELYFAICDNPVLVEDGGKSFWLEKENGKKCFMLSPKKSMWITWVSTPQYWRWISI 164

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy Swiss-Prot
Match: O80494 (F-box protein PP2-B15 OS=Arabidopsis thaliana OX=3702 GN=PP2B15 PE=2 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 2.0e-04
Identity = 22/73 (30.14%), Postives = 38/73 (52.05%), Query Frame = 0


Query: 18  YGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYWFHRKGRGNAYFIFPKAI 77
           Y H +S    +T  H + + S  ELY+ LC+   ++NG KI+   +     +Y +  + +
Sbjct: 48  YCHVIS---RSTDPHRI-FSSKKELYRCLCESILIDNGRKIFKIEKLSGKISYILSSRDL 107

Query: 78  SIAWINECQYWRW 91
           SI W ++  YW W
Sbjct: 108 SITWSDQRHYWSW 116

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy Swiss-Prot
Match: Q949S5 (F-box protein PP2-B11 OS=Arabidopsis thaliana OX=3702 GN=PP2B11 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.4e-04
Identity = 24/86 (27.91%), Postives = 40/86 (46.51%), Query Frame = 0


Query: 9   RENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKI-YWFHRKGRG 68
           R  + + + + H L    P         P+  +L+  L D   L NGT + +   RK   
Sbjct: 32  RSAAGSDDVWNHFLPADFPAGFAAPAGLPTRKQLFFSLVDNPLLINGTLLSFSLERKSGN 91

Query: 69  NAYFIFPKAISIAWINECQYWRWISI 94
             Y +  +A++I W +E +YW WIS+
Sbjct: 92  KCYMMAARALNIVWGHEQRYWHWISL 117

[Close]

BLAST of Cucsat.G8435.T8 vs. NCBI nr
Match: XP_004135560.1 (lectin [Cucumis sativus] >KGN65959.1 hypothetical protein Csa_023284 [Cucumis sativus])

HSP 1 Score: 216 bits (549), Expect = 5.28e-69
Identity = 95/97 (97.94%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MVSS+LEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNG+KIYW
Sbjct: 1  MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG
Sbjct: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. NCBI nr
Match: NP_001284457.1 (protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem lectin [Cucumis melo])

HSP 1 Score: 152 bits (385), Expect = 4.07e-44
Identity = 62/97 (63.92%), Postives = 82/97 (84.54%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EAREN + KEK+GHCLSYILP+TG+  VHWPS S+LY+QLC+G +LNNGTKIYW
Sbjct: 1  MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN YF+ PK++SIAWI++ +YW+WI +E+ G
Sbjct: 61 FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. NCBI nr
Match: XP_004135559.1 (lectin [Cucumis sativus] >KGN65960.1 hypothetical protein Csa_023237 [Cucumis sativus])

HSP 1 Score: 132 bits (333), Expect = 2.85e-36
Identity = 53/97 (54.64%), Postives = 75/97 (77.32%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+++I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. NCBI nr
Match: AAM89256.1 (26 kDa phloem protein [Cucumis sativus])

HSP 1 Score: 132 bits (333), Expect = 2.85e-36
Identity = 53/97 (54.64%), Postives = 75/97 (77.32%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+++I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. NCBI nr
Match: CDK12646.1 (phloem lectin [Cucumis sativus])

HSP 1 Score: 130 bits (328), Expect = 1.62e-35
Identity = 53/97 (54.64%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+ +I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL
Match: A0A0A0LY62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G542500 PE=4 SV=1)

HSP 1 Score: 216 bits (549), Expect = 2.55e-69
Identity = 95/97 (97.94%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MVSS+LEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNG+KIYW
Sbjct: 1  MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG
Sbjct: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL
Match: Q8LK97 (26 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1)

HSP 1 Score: 152 bits (385), Expect = 1.97e-44
Identity = 62/97 (63.92%), Postives = 82/97 (84.54%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EAREN + KEK+GHCLSYILP+TG+  VHWPS S+LY+QLC+G +LNNGTKIYW
Sbjct: 1  MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN YF+ PK++SIAWI++ +YW+WI +E+ G
Sbjct: 61 FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL
Match: A0A0A0LVN2 (Phloem protein OS=Cucumis sativus OX=3659 GN=Csa_1G542510 PE=4 SV=1)

HSP 1 Score: 132 bits (333), Expect = 1.38e-36
Identity = 53/97 (54.64%), Postives = 75/97 (77.32%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+++I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL
Match: Q8LK13 (26 kDa phloem protein OS=Cucumis sativus OX=3659 GN=PP2 PE=2 SV=1)

HSP 1 Score: 132 bits (333), Expect = 1.38e-36
Identity = 53/97 (54.64%), Postives = 75/97 (77.32%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+++I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYG 97

[Close]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL
Match: W0ZFB6 (Phloem lectin OS=Cucumis sativus OX=3659 GN=pp2 PE=2 SV=1)

HSP 1 Score: 130 bits (328), Expect = 7.83e-36
Identity = 53/97 (54.64%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 1  MVSSQLEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGTKIYW 60
          MV  + EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN GTK+YW
Sbjct: 1  MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61 FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFG 97
          F +KG+GN Y + PK+ +I WI++CQYW+WI  EI+G
Sbjct: 61 FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYG 97

The following BLAST results are available for this feature:

BLAST of Cucsat.G8435.T8 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVR5	5.1e-05	37.93	Putative F-box protein PP2-B2 OS=Arabidopsis thaliana OX=3702 GN=PP2B2 PE=4 SV=2	[more]
O80494	2.0e-04	30.14	F-box protein PP2-B15 OS=Arabidopsis thaliana OX=3702 GN=PP2B15 PE=2 SV=2	[more]
Q949S5	4.4e-04	27.91	F-box protein PP2-B11 OS=Arabidopsis thaliana OX=3702 GN=PP2B11 PE=1 SV=1	[more]

BLAST of Cucsat.G8435.T8 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135560.1	5.28e-69	97.94	lectin [Cucumis sativus] >KGN65959.1 hypothetical protein Csa_023284 [Cucumis sa...	[more]
NP_001284457.1	4.07e-44	63.92	protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem l...	[more]
XP_004135559.1	2.85e-36	54.64	lectin [Cucumis sativus] >KGN65960.1 hypothetical protein Csa_023237 [Cucumis sa...	[more]
AAM89256.1	2.85e-36	54.64	26 kDa phloem protein [Cucumis sativus]	[more]
CDK12646.1	1.62e-35	54.64	phloem lectin [Cucumis sativus]	[more]

BLAST of Cucsat.G8435.T8 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY62	2.55e-69	97.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G542500 PE=4 SV=1	[more]
Q8LK97	1.97e-44	63.92	26 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1	[more]
A0A0A0LVN2	1.38e-36	54.64	Phloem protein OS=Cucumis sativus OX=3659 GN=Csa_1G542510 PE=4 SV=1	[more]
Q8LK13	1.38e-36	54.64	26 kDa phloem protein OS=Cucumis sativus OX=3659 GN=PP2 PE=2 SV=1	[more]
W0ZFB6	7.83e-36	54.64	Phloem lectin OS=Cucumis sativus OX=3659 GN=pp2 PE=2 SV=1	[more]

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8435	Cucsat.G8435	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8435.T8.E1	Cucsat.G8435.T8.E1	exon
Cucsat.G8435.T8.E2	Cucsat.G8435.T8.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8435.T8.C2	Cucsat.G8435.T8.C2	CDS
Cucsat.G8435.T8.C1	Cucsat.G8435.T8.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8435.T8	Cucsat.G8435.T8-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0030246	carbohydrate binding