Cucsat.G8356.T11 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8356.T11
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein
Location	ctg1557: 224836 .. 227825 (-)
RNA-Seq Expression	Cucsat.G8356.T11
Synteny	Cucsat.G8356.T11

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAAAGTCATCTTTTTTAAAATTCATCTCAAAACTCTTTAGATCCTTCCAAATTATCCTATTTTTGATAATTCCCCAAGGAAATTCTCCGCCCCCAGCGGCGCCGACCACCGCTGAGTACTGCATTCATTGCGGCGGAGCGATCTCCATTCTAACTCCACGCTCAACTCTTCAATTTTAAAATTCAATCAACCAAAAAAGGAAATTGCCAACTTTTTTTTATTGTAATTTAAAAGGCTTCGATTTCACTCACTCCCTCGATGACTGTTCATGAAAAGGCGTGAAAAGAACTGAGATCGTGTCTATATCTTATAAGTCCACGATATTGCATTGCATCCTCCCATTTCTTTTTCATTCTTCAACAACTTTTTGTGTATTCTCATTGGTAATTTCTCTTCTTCAGGCGGATTTCTCCTTAGGCGGTTCTATTTGTATTTTTTGTGAGTATTGGTTTCCATTCTGATAGTTCATTTTTTAGCACCATTTGGAGAATACTTTCATGGGTTTTGGTTGTTTCGTTGGAATTTTTTTTTTCCAGTGATTTTGGGGTCAATCCACATTTTGTTATGACCATTTTCTTGTTTCATTTGCATGGGTTTGAGTTTGTTGAATCTAAATCAGTTGATAGATGAAATGCATTTTTTGTTTTTTTGTGGTTATTTTTGGGGGGATTTTGTTTAGTTTGAGATCTGTTTGATCAATGTTTTTGGGCATTGCAGATTGAAGTGTTCTTGAGTTTTGTGGGTACCTGGAAATGGAGAATGGTGATGAGTTCAATCAGATTTCTGATGCTAAATATGAATGTCTTCTCTTTGGTATTACATTTTGGACTTCTTATCTTTTTCAAGTTCAAAGTTGGATTCGAGAGTTTTTTATAACAAAGAATTATTTGTTTACTTTCAGATCTAGATGACACTCTTTATCCTTTTAACTCTGGTTTGGCAAAGGAAATCACCAAGAACATTCAAGGTTAGATGGAATTTCCTTTCCCCTTTCCCCTCTGGATTCAGAAACATTTGGGGCTGATAGTGTTTAGAGAAATGAGGCTCTTCGTATTTCTATGATATTGTCCATTTTGAACTTGAGTTTGCATGGATTTATCTTTTTGAAATGTTATCTTATGTGAATTGACATGGGATTATGTTCAAATCTCCAACTGATAACGAGTTAGAAGTTAGGTTTCTTTGTTGTTTTATTGATTGGATGAGTTTTTGTTACTTCTCTTGTAGAATATATGATTGAGAAGTTAGGAATGGAAGAGAACGTGCCTGAATTGTGCATTTCCTTGTACAAGATTTATGGGACAACAATGGCTGGTCTTAGAGTAAGTTAAAACATCATAGCCTTTTACATGATCTTTCTTCAAAACAACCATTGCAGTAAATTATTGGATACTATGTCCTGGTCGTTTTTCCTGGTTGCAGGCTATTGGGTATAACTTCGATTACGATGATTTCCATAGGTAACTAACTTTTTATCTACATTATTCTATGTTTGAGAATTTTGTGTTGTATTCACAATTCTTTTTCTTCCTGTTCTGCAGTTTTGTTCATGGAAGATTGCCATATGATATGCTCAAGCCTGACCCGTTATTGAGAAACCTACTTCACAGCTTACCAATTCGAAAATTTGTAAGTAGCTTGTTATGGAATTGACAAATGAAGAAAACAGAAAACATAAATCAATAGTGAATCAACACAGAGACATTTTAACCTCCCTTACCAGACTTTAGTTATACTATTTGGTGTCTATTTTAGTTTTAACTAGTGTTTTGATTTTTACCCTTTCTTTTTGTGTTTCTCTCTGATTTCTGACCTCAGATATTCACAAATGGTGATATGGCCCATGCAAACAGAGCGCTGAAGAGACTAGGATTAGAGGACTGCTTTGAAGGGATTTTATGCTTTGAGACTTTGAATCCTGATAAGGGAACGGTTGATGAGGAAGAAGGGTCTGTAATTTTTGACATCAATCAGTACATGAGCAATCCAAATTCTGATCTTGATCTACCGAAGACGCCGGTTGTCTGCAAACCATTCGAAGAGGCATATAAGCAAGTTTTCGAGATAGCCAATATCAACCCGAAAAAAACTGTAAGTTGAGAACAGATCAGAGTAACTTTATGTGACCTTTTCCAAGAGTTCGATGATTATAATTCTGTTGTGTTGAAAACAAAACATCCAATATTTAAAACTAGAATCAATAAACTTATTATTCATTTAATATATAAGATCTTAACAACTTACTGGGCCTGCCAAAAGCCATTCTTCTTAAGTGCCTAATACATTAACAAGAATAAATTTTAGCCTTTTAACTTGCAAAGCCTGCTATCTGACCATCAAGAACATTGATCGTTTAATACTTAGCCTGATCTGTTATCTAACTCCATTTTCATTTCACAATACTACATTCTTCTTATTTTCTTGTTTCCAGTTGTTCTTTGATGATAGTGTTCGAAATCTACAAACGGGGAAGTTGGTGGGCCTTCACACAGTGCTTGTAAGTGGCAATACCATTGGGATTTCTTTAAAGTATGCTGCTGAATTCACTTACATTCTAACCTAATCATGAATTCAGGTTGGAAATTCGCAACGAATCAAGGGGGTGGATCACGCTTTTGAGAGCATCCATAATATCAAGGAAGGATTGCCTGAGCTATGGGAAGACATGGAGAAGCTCAAGAGCGTCACATACTCCAGAAAGGAGGTAGCTATCGAGACTTCAGTTAGAGCATAATTATTAATTAGTATTTATGATCTTACTTACAAGTGGACAGTGCAAAAACTGTTGGCAATTGCTGCAATGAATGCAAAATATGAGAACTTGTTAATAGAACAATAATTATTGGCTCATGATAAGAGTGAAAATTTGTCTCCTTTTAGATTTCTGTTGATCATAAACTCTCTCTTTCTTTCTTTCTTTTCTTTCTTTATTTTCTTTTTAAGAGTTCAACATGAGGTAGAATGAAGGATTTTTCATTTTTGAAGGTT

Coding sequence (CDS)

ATGGAGAATGGTGATGAGTTCAATCAGATTTCTGATGCTAAATATGAATGTCTTCTCTTTGATCTAGATGACACTCTTTATCCTTTTAACTCTGGTTTGGCAAAGGAAATCACCAAGAACATTCAAGAATATATGATTGAGAAGTTAGGAATGGAAGAGAACGTGCCTGAATTGTGCATTTCCTTGTACAAGATTTATGGGACAACAATGGCTGGTCTTAGAGCTATTGGGTATAACTTCGATTACGATGATTTCCATAGTTTTGTTCATGGAAGATTGCCATATGATATGCTCAAGCCTGACCCGTTATTGAGAAACCTACTTCACAGCTTACCAATTCGAAAATTTATATTCACAAATGGTGATATGGCCCATGCAAACAGAGCGCTGAAGAGACTAGGATTAGAGGACTGCTTTGAAGGGATTTTATGCTTTGAGACTTTGAATCCTGATAAGGGAACGGTTGATGAGGAAGAAGGGTCTGTAATTTTTGACATCAATCAGTACATGAGCAATCCAAATTCTGATCTTGATCTACCGAAGACGCCGGTTGTCTGCAAACCATTCGAAGAGGCATATAAGCAAGTTTTCGAGATAGCCAATATCAACCCGAAAAAAACTTTGTTCTTTGATGATAGTGTTCGAAATCTACAAACGGGGAAGTTGGTGGGCCTTCACACAGTGCTTGTTGGAAATTCGCAACGAATCAAGGGGGTGGATCACGCTTTTGAGAGCATCCATAATATCAAGGAAGGATTGCCTGAGCTATGGGAAGACATGGAGAAGCTCAAGAGCGTCACATACTCCAGAAAGGAGGTAGCTATCGAGACTTCAGTTAGAGCATAA

Protein sequence

MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA

Homology

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy Swiss-Prot
Match: Q09893 (Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24B11.05 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.7e-16
Identity = 74/251 (29.48%), Postives = 111/251 (44.22%), Query Frame = 0


Query: 18  LLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGM-EENVPELCISLYKIYGTTMAGLRAI 77
           + FDLD+ LYP +  +   +   I  +  +KLG+  E    L    Y+ YG  + GL  +
Sbjct: 8   IFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGL-VL 67

Query: 78  GYNFDYDDFHSFVHGRLPYD-MLKPDPLLRNLLHSLPIRK----FIFTNGDMAHANRALK 137
            +  D  D+   V   LP + ++K D +LR +L  L +RK    +IFTN  + HANR LK
Sbjct: 68  HHEIDAVDYDQRVDQSLPLEKVIKKDEVLREML--LELRKKYKCWIFTNAYIVHANRVLK 127

Query: 138 RLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEE 197
            LG+EDCF+GI                                +  D     ++ KP  E
Sbjct: 128 YLGIEDCFDGI--------------------------------TYCDYNAKDLIAKPMPE 187

Query: 198 AYKQVFEIANINPK-KTLFFDDSVRNLQTGKLVG-LHTV-LVGNSQRI---KGVDHAFES 257
            Y++V   A +  K K +F DDS  N+   +  G  +TV LV +   +   +   H    
Sbjct: 188 MYERVMREAGVTDKDKCIFVDDSYGNILGAREFGWKYTVQLVEHGDPLPQPQAGSHVIRD 223

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy Swiss-Prot
Match: P40025 (Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PHM8 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.0e-10
Identity = 45/140 (32.14%), Postives = 74/140 (52.86%), Query Frame = 0


Query: 12  DAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI-SLYKIYGTTM 71
           D   +   FD+D+TLY  ++ +   + +++  +   +LG +++  E  I S Y+ YG ++
Sbjct: 49  DPARKVFFFDIDNTLYRKSTKVQLLMQQSLSNFFKYELGFDDDEAERLIESYYQEYGLSV 108

Query: 72  AGLRAIGYNFDYDDFHSFVHGRLP-YDMLKPDPLLRNLLHSLPIRK-------FIFTNGD 131
            GL       D   +++F+   LP  D LKPD  LR LL +L  +K       ++FTN  
Sbjct: 109 KGLIKNKQIDDVLQYNTFIDDSLPLQDYLKPDWKLRELLINLKKKKLGKFDKLWLFTNSY 168

Query: 132 MAHANRALKRLGLEDCFEGI 143
             HA R +K LG+ D F+GI
Sbjct: 169 KNHAIRCVKILGIADLFDGI 188

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy Swiss-Prot
Match: P53078 (Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDT1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.6e-10
Identity = 57/218 (26.15%), Postives = 100/218 (45.87%), Query Frame = 0


Query: 20  FDLDDTLYPFNSGLAKEITKNIQEYMIEKLGME-ENVPELCISLYKIYGTTMAGLRAIGY 79
           FD+D+ LY  ++ +   + ++I  +    L +  E+   L  S YK YG  + GL  + +
Sbjct: 60  FDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLAIRGL-VMFH 119

Query: 80  NFDYDDFHSFVHGRLP-YDMLKPDPLLRNLLHSLP-----IRKFIFTNGDMAHANRALKR 139
             +  +++  V   LP  D+LKPD  LRN+L  L       + ++FTN    HA R L+ 
Sbjct: 120 KVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYKNHAIRCLRL 179

Query: 140 LGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEA 199
           LG+ D F+G+                          Y     +D       +VCKP  +A
Sbjct: 180 LGIADLFDGL-------------------------TYCDYSRTD------TLVCKPHVKA 239

Query: 200 YKQVFEIANI-NPKKTLFFDDSVRNLQTGKLVGLHTVL 230
           +++  + + +   +   F DDS +N++TG  +G+ T +
Sbjct: 240 FEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCI 245

[Close]

BLAST of Cucsat.G8356.T11 vs. NCBI nr
Match: XP_011658227.1 (uncharacterized protein C24B11.05 [Cucumis sativus] >XP_011658231.1 uncharacterized protein C24B11.05 [Cucumis sativus] >KGN65738.1 hypothetical protein Csa_019919 [Cucumis sativus])

HSP 1 Score: 576 bits (1484), Expect = 1.99e-207
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

[Close]

BLAST of Cucsat.G8356.T11 vs. NCBI nr
Match: XP_016902092.1 (PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902093.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902094.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo])

HSP 1 Score: 557 bits (1436), Expect = 4.14e-200
Identity = 274/282 (97.16%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDL 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 281

[Close]

BLAST of Cucsat.G8356.T11 vs. NCBI nr
Match: TYJ97389.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 553 bits (1426), Expect = 1.68e-195
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 477

[Close]

BLAST of Cucsat.G8356.T11 vs. NCBI nr
Match: XP_038893602.1 (uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893603.1 uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893604.1 uncharacterized protein LOC120082486 [Benincasa hispida])

HSP 1 Score: 543 bits (1400), Expect = 1.23e-194
Identity = 265/281 (94.31%), Postives = 273/281 (97.15%), Query Frame = 0


Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGD+FNQISDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDQFNQISDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDP+LRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPVLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180
           GDMAHA RALKRLGLEDCFEGILCFETLNPDKGTVDEE GSVIF+INQYMSNPNSD+DLP
Sbjct: 121 GDMAHATRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFNINQYMSNPNSDIDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEA+KQVFEIANINP+KTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAFKQVFEIANINPQKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           HAFESIHNIKEGLPELWE MEKL S++YS K VAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEAMEKLNSISYSEK-VAIETSVRA 280

[Close]

BLAST of Cucsat.G8356.T11 vs. NCBI nr
Match: KAA0067755.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 553 bits (1426), Expect = 2.79e-194
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 558

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL
Match: A0A0A0LVD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523620 PE=4 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 9.63e-208
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL
Match: A0A1S4E1J3 (uncharacterized protein C24B11.05-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497146 PE=4 SV=1)

HSP 1 Score: 557 bits (1436), Expect = 2.00e-200
Identity = 274/282 (97.16%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDL 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 281

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL
Match: A0A5D3BED8 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002080 PE=4 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 8.13e-196
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 477

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL
Match: A0A5A7VPY6 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00750 PE=4 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 1.35e-194
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0


Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 558

[Close]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL
Match: A0A1S4E293 (uncharacterized protein C24B11.05-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497146 PE=4 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 3.12e-184
Identity = 254/262 (96.95%), Postives = 259/262 (98.85%), Query Frame = 0


Query: 21  DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLRAIGYNF 80
           DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGL+AIGYNF
Sbjct: 4   DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLKAIGYNF 63

Query: 81  DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDMAHANRALKRLGLEDCFE 140
           DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGDMAHANRALKRLGLEDCFE
Sbjct: 64  DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGDMAHANRALKRLGLEDCFE 123

Query: 141 GILCFETLNPDKGTVDEEE-GSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEAYKQVFEI 200
           GILCFETLNPDKGTVDEEE GSVIFDINQYMSNPNSD+DLPKTPVVCKPFEEA+KQVFE+
Sbjct: 124 GILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPKTPVVCKPFEEAFKQVFEM 183

Query: 201 ANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDHAFESIHNIKEGLPELWED 260
           ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDHAFESIHNIKEGLPELWED
Sbjct: 184 ANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDHAFESIHNIKEGLPELWED 243

Query: 261 MEKLKSVTYSRKEVAIETSVRA 281
           MEKLKSVTYSRK VAIETSVRA
Sbjct: 244 MEKLKSVTYSRK-VAIETSVRA 264

The following BLAST results are available for this feature:

BLAST of Cucsat.G8356.T11 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09893	4.7e-16	29.48	Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]
P40025	5.0e-10	32.14	Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
P53078	6.6e-10	26.15	Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 20450...	[more]

BLAST of Cucsat.G8356.T11 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011658227.1	1.99e-207	100.00	uncharacterized protein C24B11.05 [Cucumis sativus] >XP_011658231.1 uncharacteri...	[more]
XP_016902092.1	4.14e-200	97.16	PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_...	[more]
TYJ97389.1	1.68e-195	97.14	Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. maku...	[more]
XP_038893602.1	1.23e-194	94.31	uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893603.1 unchara...	[more]
KAA0067755.1	2.79e-194	97.14	Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. maku...	[more]

BLAST of Cucsat.G8356.T11 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVD2	9.63e-208	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523620 PE=4 SV=1	[more]
A0A1S4E1J3	2.00e-200	97.16	uncharacterized protein C24B11.05-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5D3BED8	8.13e-196	97.14	Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. ma...	[more]
A0A5A7VPY6	1.35e-194	97.14	Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. ma...	[more]
A0A1S4E293	3.12e-184	96.95	uncharacterized protein C24B11.05-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 34..54
None	No IPR available	SFLD	SFLDS00003	Haloacid_Dehalogenase	coord: 12..263 e-value: 0.0 score: 229.2
None	No IPR available	PANTHER	PTHR12725	HALOACID DEHALOGENASE-LIKE HYDROLASE	coord: 1..281
None	No IPR available	PANTHER	PTHR12725:SF72	HALOACID DEHALOGENASE-LIKE HYDROLASE	coord: 1..281
IPR010237	Pyrimidine 5-nucleotidase	TIGRFAM	TIGR01993	TIGR01993	coord: 16..230 e-value: 2.4E-66 score: 221.0
IPR010237	Pyrimidine 5-nucleotidase	SFLD	SFLDG01132	C1.5.3:_5'-Nucleotidase_Like	coord: 12..263 e-value: 0.0 score: 229.2
IPR010237	Pyrimidine 5-nucleotidase	CDD	cd02604	HAD_5NT	coord: 17..232 e-value: 2.23333E-79 score: 236.376
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 78..257 e-value: 1.7E-42 score: 146.7
IPR006439	HAD hydrolase, subfamily IA	TIGRFAM	TIGR01509	TIGR01509	coord: 183..230 e-value: 3.8E-11 score: 41.4
IPR041492	Haloacid dehalogenase-like hydrolase	PFAM	PF13419	HAD_2	coord: 18..152 e-value: 3.6E-5 score: 23.9
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 17..234

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8356	Cucsat.G8356	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8356.T11.E1	Cucsat.G8356.T11.E1	exon
Cucsat.G8356.T11.E2	Cucsat.G8356.T11.E2	exon
Cucsat.G8356.T11.E3	Cucsat.G8356.T11.E3	exon
Cucsat.G8356.T11.E4	Cucsat.G8356.T11.E4	exon
Cucsat.G8356.T11.E5	Cucsat.G8356.T11.E5	exon
Cucsat.G8356.T11.E6	Cucsat.G8356.T11.E6	exon
Cucsat.G8356.T11.E7	Cucsat.G8356.T11.E7	exon
Cucsat.G8356.T11.E8	Cucsat.G8356.T11.E8	exon
Cucsat.G8356.T11.E9	Cucsat.G8356.T11.E9	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8356.T11.C8	Cucsat.G8356.T11.C8	CDS
Cucsat.G8356.T11.C7	Cucsat.G8356.T11.C7	CDS
Cucsat.G8356.T11.C6	Cucsat.G8356.T11.C6	CDS
Cucsat.G8356.T11.C5	Cucsat.G8356.T11.C5	CDS
Cucsat.G8356.T11.C4	Cucsat.G8356.T11.C4	CDS
Cucsat.G8356.T11.C3	Cucsat.G8356.T11.C3	CDS
Cucsat.G8356.T11.C2	Cucsat.G8356.T11.C2	CDS
Cucsat.G8356.T11.C1	Cucsat.G8356.T11.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8356.T11	Cucsat.G8356.T11-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity