Cucsat.G8305.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G8305.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCASP-like protein
Locationctg1557: 4682899 .. 4686287 (+)
RNA-Seq ExpressionCucsat.G8305.T4
SyntenyCucsat.G8305.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAATATGGACATTTTTAATTTTGGTGTTATGTAAATATTTTTCCTAAAATTAAGGGAATGTGTAAATACTTTTTTTTTTCTTTAGAAAAATATACAAAAAGAAGCATATAGGTTCCAAGGAGAAAATTGGAAGAGATTGGTGTAAAAATATTGTTGTTCGTTCTTTTTCTTCTAGTTAGAGAGTCCACTCACCAAAGTGCAGCTTCCATGGCAGCATCAGCATCAGCATCGAACTCTGAAGCAGAAATGAAAAAGGAAGAACTACCAAAATCCATGGAAGAACCACCAGAACAAGAACATCCCCACCATAACCAATCAGATGAAGGCGAAGACGACAACGAATCCCATCTCCATCTCTCTGCCTGCTCTTCACCTCCTCATTCCCTCTCCACCAACAAAGATCCCTCGCCGCCTCTCCACTCTCCCTCCATCTCCTCCGATTTCTCCGATCACACTTGTCACTCTCCTGGAAACTCCTCCATCGACCACGCCCTCTCCATTACACCCCTTCAGGACTTACACTTACCTCCCTCTGCTAATTCTCCATCTCCAAGACCTGTAGCAGCCAACCGCGCGCAGCCGTCTGAGCCGATTGTTGTGACCAAGGTGGATACTGAAATACAGGGAATACGGAAAGTGGAAGAGGTTATTGATAGTGATGGCGATGTTGAGAGTGGCGATGGAGATGATGGAGATGGTGGTAAGGTTGGGCGAGGACGGAAATTGATGGAGACTTTGTCGATTAAGAAAATTAAGAGGGAGGAATTGAGGAAGAAGATCTTGTTAGGGTTTAGAATTTGTGGATTTGCCTTCTGTTTGGTATCGGTTTCGGTTATGGCTTCTGATAAGGATCAGGGCTGGGCTTTGGATTCATTCTATCGCTATAAGGAATTCAGGTAATTAGTTTGTTCAATGAAATCGTTTCTTACTAATGAAATATCAAATTTTATGGAGTTATTCAATTTGGAAATTTCATTAAATTTGTAGGTACTGTATGGCAGTCAATGTTATTGGATTTGTTTACTCTGCACTTCAATCCTATGATCTTGTCTATTTTCTCTCTACTGGAAAACACGTGATCCGTAATCACTTCAAGCAATACTTTGATTTCTTCATTGATCAGGCAAGTTTCTGTTTTGGCTTGAAATTGGGTTTCTGAATTTCAATCTGTTCATTATTAATTCTGCTATAATCTCATTACCTAACCCAAAAATGGATGGCTAAGATTAAGCCAATAGTTTAGTCCTTAATTTAGATGACATTATTGTGTATCGATTATGATAGCTTATAGGACCCACTGAAGATAGAAAAGTTATGCATAATTCCCAACAGTCATTAGATAGATGGTTCTCTTTCTGTATTCCTTTACTGAAAATTTTTCCATTCATCATGAAGCTTTTTCTTACCTTTTTCATACAGAAGAATCTGCAAGTTCTTCAATATTTAACATCTTCCTGCCGTCTTTAACACCCAATATTTAACATCTTACTGACGTCTTTAACACCCAATATTTAACATCTTACTGACGTCTTTAACACCCAAATGTCATAGTGTTAGATGGGTTATCCGAGAAAGAAAAATAGATATAATCTGCAAGCGCTCTCCATGAAGAGCTTACTAATACTTTTACCATAAAGTCACTCAATTTTTATATTAATAACAGAGTAGTGGTTGGAAAAACTTGGATTGATTTGGTTTGGTTGAGTAACTTTCCTTATATTCTTTCCATTTTATTTGCCTTAGCTTTTGGGCATCCTTTTTATTACCTTTTCTCTGCTACTGCCGCCTTTCCCTCGCATTTTCTTATTTTTCCTTTTTATTCTTAAAGTTCGTAAGGAAACTAGCTTTAATTTGCAGCAAAGAATCTAGTATGGACATTAATAATCACTTGGCTGTAAAAAATTAAAGAAAAATTAACTTCCGGCCAAACCTTGTATTCTAAACCGATAAAAGAGAGCATATCTCAAGTGAGATATGTAGGGGTGTGCATCAATTTTCAGATCTAACTGCCACCAAACTGACTATTATCGATTTAGCAAATGTTCAAATTGACCTTGATCATTGGGGAGTGCAAATCAGTTGTCGGTCGGTATTATCCTTTAGTTTTCTTTTTAAAAAATGTCGTTGTGGACTTCCCCAAACCGATGCTAATCGAGCCCCTCCTTATCTCCGATCAACCACCTTTGTTTTTCGAATTTGGTCTATCATGCTAACTCGTAGACATATGTCTTAGCCACCAAGAGGTTTTGAGTTCGATTCATCTACCCTTGTTATGCACTTGAAAAAAAAAGAAAATTTCCTATGAAGTTGTAAAATCGTTGCAATTAATTCTTATTACACTTGTGCTATAAGTGCTTGAGTTTTAATAGTCATCTGACTTAAAAAGTTCAATGGTGTTAGTGCAAAACAGTCCTTGTAATTTAAGAATGATTTAAGGTTTGGAATTGAAGTGTAACGACCTTAAACCTTGTGGTTAGAGGCAGGGCTGCACATTTTTCAAGTTGCAACTATATGGAGTTAGGATGACTAAGTTGAATGATTGTTTAAAGTGAAAAAGCTAAGATACAATTGTAATAACTCTTGTAGTCAAAGGTAGTTTTTGCATTTTTTTTCATAGACTAACCATTAACTTTATGGATAGAACTGAGTCATGATTCAAACATTCAATTTCACATGAAGTTACTAATAAACATTTCGTTAGTGTTCGAGGTTCACTACACTTGGTGCGATAATCATAAAGAAATTGATGACAGCGTTGTTCTGCTACAGATAATAGCATATCTTCTTCTATCAGCTTCGTCTTCGGCTGCCACCCGAATCGATGATTGGCAATCCAATTGGGGAAAGGACAAGTTTCCAGATATGGCTACCGGGTCGTTGGGATTTTCCATTGTTGCATTTGTAGCTTTTGCCTTGAGTTGTATAATCTCTGGTTATACTCTATGTAAATCTGCTTAGGTAACCTTTGCTGGACTTGCTTATGGGCGAACTTTATTCAATGATGATGATACCAACAAACAAACAAACACAATGGTGATGAGGATATACAAGAAGATTAGTTTCAACATTGTGCTGTGAATCAGGTATGCTTTCACTTGAAAAATATATTTGTGAGTTTACTTAATTCTTTGTAAATAAGATGAAGAACACTTGTTGTGCACTTTGAATCATTACAAGGTGAAAAGGAAAGAGTGAATAGGGAAAAGTTAGAGAAAGTTATTCAATGTTTTCATAAATTGCTAAAATGGAGAAAGCCATGACTCAATTACATGATAAAACAAGTATCCAAAACAAGGGTAAGTTAATAATAATAAAGACGTTTTTGGTTGACAATCCAAATTTTAGTTTAGGTTCTAAAATCTACGGAGTTCAAGATTCTATTATCTA

Coding sequence (CDS)

ATGGCAGCATCAGCATCAGCATCGAACTCTGAAGCAGAAATGAAAAAGGAAGAACTACCAAAATCCATGGAAGAACCACCAGAACAAGAACATCCCCACCATAACCAATCAGATGAAGGCGAAGACGACAACGAATCCCATCTCCATCTCTCTGCCTGCTCTTCACCTCCTCATTCCCTCTCCACCAACAAAGATCCCTCGCCGCCTCTCCACTCTCCCTCCATCTCCTCCGATTTCTCCGATCACACTTGTCACTCTCCTGGAAACTCCTCCATCGACCACGCCCTCTCCATTACACCCCTTCAGGACTTACACTTACCTCCCTCTGCTAATTCTCCATCTCCAAGACCTGTAGCAGCCAACCGCGCGCAGCCGTCTGAGCCGATTGTTGTGACCAAGGTGGATACTGAAATACAGGGAATACGGAAAGTGGAAGAGGTTATTGATAGTGATGGCGATGTTGAGAGTGGCGATGGAGATGATGGAGATGGTGGTAAGGTTGGGCGAGGACGGAAATTGATGGAGACTTTGTCGATTAAGAAAATTAAGAGGGAGGAATTGAGGAAGAAGATCTTGTTAGGGTTTAGAATTTGTGGATTTGCCTTCTGTTTGGTATCGGTTTCGGTTATGGCTTCTGATAAGGATCAGGGCTGGGCTTTGGATTCATTCTATCGCTATAAGGAATTCAGGTACTGTATGGCAGTCAATGTTATTGGATTTGTTTACTCTGCACTTCAATCCTATGATCTTGTCTATTTTCTCTCTACTGGAAAACACGTGATCCGTAATCACTTCAAGCAATACTTTGATTTCTTCATTGATCAGGCAAGTTTCTGTTTTGGCTTGAAATTGGGTTTCTGA

Protein sequence

MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSLSTNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQASFCFGLKLGF
Homology
BLAST of Cucsat.G8305.T4 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-22
Identity = 52/96 (54.17%), Postives = 66/96 (68.75%), Query Frame = 0

Query: 180 KKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIG 239
           ++ K   L +K LLGFR+  F  CLVS SVM SD+D+GWA DSFY YKEFR+C+A NVIG
Sbjct: 111 RESKWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIG 170

Query: 240 FVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 276
           FVYS     DLVY LST     R++ + + +F +DQ
Sbjct: 171 FVYSGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQ 206

BLAST of Cucsat.G8305.T4 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.0e-21
Identity = 79/227 (34.80%), Postives = 120/227 (52.86%), Query Frame = 0

Query: 54  SSPPHSLSTNKDPSPPLHSPSISSDFSDHTCH---SPG-NSSIDHALSITPLQDLHLPPS 113
           S  P S+ST K P P   S +I + F + T H   SP    S+DH+       +   P S
Sbjct: 3   SMSPSSISTEKSPPPSDTSMAIVA-FDNSTTHLSSSPSPPHSLDHSSDSEKEDEKRRPES 62

Query: 114 ANSPSPRPVAANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGR 173
             + +P  +     +   PIVV          R V+EV+ +      G G          
Sbjct: 63  RRNKNPVKI----EETPSPIVVVHNHN-----RSVKEVVPTRKTARVGSGRSS------- 122

Query: 174 GRKLMETLSI-KKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKE 233
           G++    L+I ++ +REE+ K + LGFR+      L+S S+MA+DK +GW+ DSF RYKE
Sbjct: 123 GQRSGAVLAILRRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKE 182

Query: 234 FRYCMAVNVIGFVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 276
           +R+C++VNV+ F+Y++ Q+ DL Y L   KH+I +H +  F+F IDQ
Sbjct: 183 YRFCLSVNVVAFIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQ 212

BLAST of Cucsat.G8305.T4 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.5e-20
Identity = 81/226 (35.84%), Postives = 117/226 (51.77%), Query Frame = 0

Query: 54  SSPPHSLSTNKDPSPPLHSPSISSDFSDHTCH---SPG-NSSIDHALSITPLQDLHLPPS 113
           S  P S+ST K P P   S +I + F + T H   SP    S+DH+           P S
Sbjct: 10  SMSPSSVSTEKSPPPSDTSMAIVA-FDNSTTHFSSSPSPPHSLDHSSESEKEDAKSKPES 69

Query: 114 ANSPSPRPVAANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGR 173
             + +P  V     +   PIVV          R V+EV+ +      G G    G + G 
Sbjct: 70  RRNKNPGKV----EETPSPIVVVHNHN-----RSVKEVVPTRKSARVGSG-RSSGQRSGA 129

Query: 174 GRKLMETLSIKKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEF 233
              +     +++ +REE+ K   LGFR+      L+S S+MA+DK +GW+ DSF RYKE+
Sbjct: 130 VSAI-----LRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEY 189

Query: 234 RYCMAVNVIGFVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 276
           R+C++VNV+ FVYS+ Q+ DL Y L   KH+I +H +  F+F IDQ
Sbjct: 190 RFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQ 219

BLAST of Cucsat.G8305.T4 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.1e-15
Identity = 76/238 (31.93%), Postives = 110/238 (46.22%), Query Frame = 0

Query: 54  SSPPHSLSTNKDPSPPL--HSP-SISSDFSDHTCHSPGNS--SIDHALSI---------T 113
           SS   + S N+ P  PL  HSP S + D  +    SP  S   I++  SI         +
Sbjct: 8   SSNSEAYSYNESPHSPLRFHSPLSDAGDLPESRYVSPEGSPFKIENPKSIVAGNKLTQFS 67

Query: 114 PLQDLHLPPSANSPSPRPVAANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDG 173
           PL     PP    P PR     R   +  +  +     +Q     E            DG
Sbjct: 68  PLPSPIPPPPPQIPPPRRQRNARVPMNSSLDKSPSSMVVQNSWVRE------------DG 127

Query: 174 DDGDGGKVGRGRKLME--TLSIKKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQG 233
                 K G      E  T ++ + +R++L     LGFRI     C++S S+MA+DK QG
Sbjct: 128 QQNTTRKAGAPMNGEESATTAVNRARRDDLVSVTALGFRITEVILCVISFSIMAADKTQG 187

Query: 234 WALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 276
           W+ DS+ RYKE+RYC+AVNVI FVYSA ++ D   +++   +++   F   F F +DQ
Sbjct: 188 WSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGFHDLFVFSMDQ 233

BLAST of Cucsat.G8305.T4 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.0e-13
Identity = 75/236 (31.78%), Postives = 111/236 (47.03%), Query Frame = 0

Query: 54  SSPPHSLSTNKDPSPPL--HSP-SISSDFSDHTCHSPGNS--SIDHALSI---------T 113
           SS   + S N+ P  PL  HSP S + D  +    SP  S   I++  SI         +
Sbjct: 8   SSNSEAQSYNESPHSPLRFHSPLSDAGDLPESRYVSPEGSPFKIENPKSIVAGNKLTQFS 67

Query: 114 PLQDLHLPPSANSPSPRPVAANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDG 173
           PL     PP    P PR     R + +   + +  D     +      +  DG    G  
Sbjct: 68  PLPSPIPPPPPQFPPPR-----RQRNARVPMNSSSDKSPSSMVVHNSWVREDG----GQT 127

Query: 174 DDGDGGKVGRGRKLMETLSIKKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWA 233
                G    G +   T ++ + + ++L     LGFRI     C++S S+MA+DK QGW+
Sbjct: 128 TTRKAGAPINGEESTRT-TVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGWS 187

Query: 234 LDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 276
            DS+ RYKE+RYC+AVNVI FVYSA ++ D   +++   ++I   F   F F +DQ
Sbjct: 188 GDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQ 233

BLAST of Cucsat.G8305.T4 vs. NCBI nr
Match: XP_004142358.1 (CASP-like protein 4A3 [Cucumis sativus] >KGN52378.1 hypothetical protein Csa_008911 [Cucumis sativus])

HSP 1 Score: 530 bits (1366), Expect = 1.85e-188
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL 60
           MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL
Sbjct: 1   MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL 60

Query: 61  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 120
           STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA
Sbjct: 61  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 120

Query: 121 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 180
           NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK
Sbjct: 121 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 180

Query: 181 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 240
           KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF
Sbjct: 181 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 240

Query: 241 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ
Sbjct: 241 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275

BLAST of Cucsat.G8305.T4 vs. NCBI nr
Match: XP_008458753.1 (PREDICTED: CASP-like protein 4A1 [Cucumis melo] >KAA0037507.1 CASP-like protein 4A1 [Cucumis melo var. makuwa] >TYK01982.1 CASP-like protein 4A1 [Cucumis melo var. makuwa])

HSP 1 Score: 446 bits (1146), Expect = 3.64e-155
Identity = 242/273 (88.64%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 5   ASASNSEAEMKKEELPKSMEEPPEQEH--PHHNQSDEGEDDNESHLHLSACSSPPHSLST 64
           ASASNSEAEMK+EELPKSMEEP EQ H  PHHNQSDEGE+DNESHLHLSACSSPPHSLST
Sbjct: 3   ASASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLST 62

Query: 65  NKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANR 124
           +KDPSPPLHSPSISSDFSDHTCHS GNSSID       +QD  LPPSAN PSPRPVAANR
Sbjct: 63  SKDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQD--LPPSANPPSPRPVAANR 122

Query: 125 AQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKI 184
           AQPSEPIVVTKVDTEIQG+RKVEEV DSDGDVES DG     GKVGRGRKLME+LSIKKI
Sbjct: 123 AQPSEPIVVTKVDTEIQGVRKVEEVSDSDGDVESADG-----GKVGRGRKLMESLSIKKI 182

Query: 185 KREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 244
           K EELRKKILLGFRICGF+FCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY
Sbjct: 183 KEEELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 242

Query: 245 SALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           SALQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ
Sbjct: 243 SALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ 262

BLAST of Cucsat.G8305.T4 vs. NCBI nr
Match: XP_038890250.1 (CASP-like protein 4A1 [Benincasa hispida])

HSP 1 Score: 411 bits (1056), Expect = 2.46e-141
Identity = 225/276 (81.52%), Postives = 243/276 (88.04%), Query Frame = 0

Query: 7   ASNSEAEMKKEELPKSMEEPPE-------QEHPHHNQSDEGEDDNESHLHLSACSSPPHS 66
           AS+SEAEMKKEE  K+MEE  E       Q+  HHNQS+E E+D+ESH  LSA SSPPHS
Sbjct: 3   ASSSEAEMKKEEQAKTMEEEEEEQQQQQQQQKHHHNQSEENENDDESHPPLSARSSPPHS 62

Query: 67  LSTNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVA 126
           LS++KDPSPPLHSPSISSDFSDHTCH+ GNSSID ALSIT L+D  LPPSAN PSPRPVA
Sbjct: 63  LSSSKDPSPPLHSPSISSDFSDHTCHTHGNSSIDDALSITRLED--LPPSANPPSPRPVA 122

Query: 127 ANRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSI 186
           ANRAQP+EPIVVTKVD EIQG+RKVEEV DSDGDVESGDG     GKVGRGRKLM +LS+
Sbjct: 123 ANRAQPTEPIVVTKVDAEIQGVRKVEEVSDSDGDVESGDG-----GKVGRGRKLMASLSM 182

Query: 187 KKIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIG 246
           KK+KREE RKKILLGFRICGFAFCLVS SVMASDK+QGWALDSFYRYKEFRYCMAVN+IG
Sbjct: 183 KKMKREESRKKILLGFRICGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNIIG 242

Query: 247 FVYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           FVYSALQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ
Sbjct: 243 FVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ 271

BLAST of Cucsat.G8305.T4 vs. NCBI nr
Match: KAG7016184.1 (CASP-like protein 4A1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377 bits (967), Expect = 2.15e-128
Identity = 214/279 (76.70%), Postives = 235/279 (84.23%), Query Frame = 0

Query: 7   ASNSEAEMKKEELPKSMEEPPEQEHPHHNQSD--EGEDDNESHLHLSACSSPPHSLSTNK 66
           ASNSEAE+KKEEL ++ME+   Q+   H+QS+  + E+D+E H  LSA SSPPHSLS NK
Sbjct: 3   ASNSEAEVKKEELAETMEQ---QQLHRHSQSEVKDNENDDEFHPPLSAQSSPPHSLSDNK 62

Query: 67  DPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQ 126
           DPSPPLHSPS SSDFSDHTC + GNSSID ALSIT L+D  LPPSA  PSPRPVAANRAQ
Sbjct: 63  DPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLED--LPPSAYPPSPRPVAANRAQ 122

Query: 127 PSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKIKR 186
           P+EPIVVTKVD EIQG+RKVEE   S  DVE  DGD   GG VGRGRKLM  LS+KKIKR
Sbjct: 123 PTEPIVVTKVDAEIQGVRKVEE--GSASDVEPSDGD---GGPVGRGRKLMANLSMKKIKR 182

Query: 187 EELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 246
           EELRKKILLGFRI GF FCL S SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 183 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 242

Query: 247 LQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQASFCFGLK 283
           LQSYDL+YFLSTGKH+IRN+FKQYFDFFIDQASF  G++
Sbjct: 243 LQSYDLIYFLSTGKHMIRNYFKQYFDFFIDQASFWSGIE 271

BLAST of Cucsat.G8305.T4 vs. NCBI nr
Match: KAG6578643.1 (CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 371 bits (953), Expect = 8.43e-126
Identity = 211/272 (77.57%), Postives = 230/272 (84.56%), Query Frame = 0

Query: 7   ASNSEAEMKKEELPKSMEEPPEQEHPHHNQSD--EGEDDNESHLHLSACSSPPHSLSTNK 66
           ASNSEAE+KKEEL ++ME+   Q+   H+QS+  + E+D+ESH  LSA SSPPHSLS NK
Sbjct: 3   ASNSEAEVKKEELAETMEQ---QQLHRHSQSEVNDNENDDESHPPLSAQSSPPHSLSDNK 62

Query: 67  DPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQ 126
           DPSPPLHSPS SSDFSDHTC + GNSSID ALSIT L+D  LPPSA  PSPRPVAANRAQ
Sbjct: 63  DPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLED--LPPSAYPPSPRPVAANRAQ 122

Query: 127 PSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKIKR 186
           P+EPIVVTKVD EIQG+RKVEE   S  DVE  DGD   GG VGRGRKLM  LS+KKIKR
Sbjct: 123 PTEPIVVTKVDAEIQGVRKVEE--GSASDVEPSDGD---GGPVGRGRKLMANLSMKKIKR 182

Query: 187 EELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 246
           EELRKKILLGFRI GF FCL S SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 183 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 242

Query: 247 LQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQA 276
           LQSYDL+YFLSTGKH+I N+FKQYFDFFIDQA
Sbjct: 243 LQSYDLIYFLSTGKHMIHNYFKQYFDFFIDQA 264

BLAST of Cucsat.G8305.T4 vs. ExPASy TrEMBL
Match: A0A0A0KS01 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 8.94e-189
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL 60
           MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL
Sbjct: 1   MAASASASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSL 60

Query: 61  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 120
           STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA
Sbjct: 61  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 120

Query: 121 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 180
           NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK
Sbjct: 121 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 180

Query: 181 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 240
           KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF
Sbjct: 181 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 240

Query: 241 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ
Sbjct: 241 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275

BLAST of Cucsat.G8305.T4 vs. ExPASy TrEMBL
Match: A0A5A7T7H8 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00890 PE=3 SV=1)

HSP 1 Score: 446 bits (1146), Expect = 1.76e-155
Identity = 242/273 (88.64%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 5   ASASNSEAEMKKEELPKSMEEPPEQEH--PHHNQSDEGEDDNESHLHLSACSSPPHSLST 64
           ASASNSEAEMK+EELPKSMEEP EQ H  PHHNQSDEGE+DNESHLHLSACSSPPHSLST
Sbjct: 3   ASASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLST 62

Query: 65  NKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANR 124
           +KDPSPPLHSPSISSDFSDHTCHS GNSSID       +QD  LPPSAN PSPRPVAANR
Sbjct: 63  SKDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQD--LPPSANPPSPRPVAANR 122

Query: 125 AQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKI 184
           AQPSEPIVVTKVDTEIQG+RKVEEV DSDGDVES DG     GKVGRGRKLME+LSIKKI
Sbjct: 123 AQPSEPIVVTKVDTEIQGVRKVEEVSDSDGDVESADG-----GKVGRGRKLMESLSIKKI 182

Query: 185 KREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 244
           K EELRKKILLGFRICGF+FCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY
Sbjct: 183 KEEELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 242

Query: 245 SALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           SALQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ
Sbjct: 243 SALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ 262

BLAST of Cucsat.G8305.T4 vs. ExPASy TrEMBL
Match: A0A1S3C844 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498066 PE=3 SV=1)

HSP 1 Score: 446 bits (1146), Expect = 1.76e-155
Identity = 242/273 (88.64%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 5   ASASNSEAEMKKEELPKSMEEPPEQEH--PHHNQSDEGEDDNESHLHLSACSSPPHSLST 64
           ASASNSEAEMK+EELPKSMEEP EQ H  PHHNQSDEGE+DNESHLHLSACSSPPHSLST
Sbjct: 3   ASASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLST 62

Query: 65  NKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANR 124
           +KDPSPPLHSPSISSDFSDHTCHS GNSSID       +QD  LPPSAN PSPRPVAANR
Sbjct: 63  SKDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQD--LPPSANPPSPRPVAANR 122

Query: 125 AQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKI 184
           AQPSEPIVVTKVDTEIQG+RKVEEV DSDGDVES DG     GKVGRGRKLME+LSIKKI
Sbjct: 123 AQPSEPIVVTKVDTEIQGVRKVEEVSDSDGDVESADG-----GKVGRGRKLMESLSIKKI 182

Query: 185 KREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 244
           K EELRKKILLGFRICGF+FCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY
Sbjct: 183 KEEELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVY 242

Query: 245 SALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           SALQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ
Sbjct: 243 SALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ 262

BLAST of Cucsat.G8305.T4 vs. ExPASy TrEMBL
Match: A0A6J1FJD7 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1)

HSP 1 Score: 366 bits (940), Expect = 4.12e-124
Identity = 208/270 (77.04%), Postives = 225/270 (83.33%), Query Frame = 0

Query: 7   ASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEG-EDDNESHLHLSACSSPPHSLSTNKD 66
           ASNSEAE+KKEEL ++ME+     H     ++ G E+D+ESH  LSA SSP HSLS NKD
Sbjct: 3   ASNSEAEVKKEELAETMEQQQLHRHSQSEVNENGNENDDESHPPLSAQSSPAHSLSDNKD 62

Query: 67  PSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQP 126
           PSPPLHSPS SSDFSDHTC + GNSSID ALSIT L+D  LPPSA  PSPRPVAANRAQP
Sbjct: 63  PSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLED--LPPSAYPPSPRPVAANRAQP 122

Query: 127 SEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIKKIKRE 186
           +EPIVVTKVD EIQG+RKVEE   S  DVE  DGD   GG VGRGRKLM  LS+KKIKRE
Sbjct: 123 TEPIVVTKVDAEIQGVRKVEE--GSASDVEPSDGD---GGPVGRGRKLMANLSMKKIKRE 182

Query: 187 ELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSAL 246
           ELRKKILLGFRI GF FCL S SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGFVYSAL
Sbjct: 183 ELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSAL 242

Query: 247 QSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           QSYDL+YFLSTGKH+IRN+FKQYFDFFIDQ
Sbjct: 243 QSYDLIYFLSTGKHMIRNYFKQYFDFFIDQ 265

BLAST of Cucsat.G8305.T4 vs. ExPASy TrEMBL
Match: A0A6J1JTE4 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1)

HSP 1 Score: 362 bits (930), Expect = 1.46e-122
Identity = 209/275 (76.00%), Postives = 228/275 (82.91%), Query Frame = 0

Query: 7   ASNSEAEMKKEELPKSMEEPPEQEHPHHNQSD------EGEDDNESHLHLSACSSPPHSL 66
           ASNSEAE+KKEEL ++ME+   Q+   HNQS+      E E+D+ESH  LSA SSPPHSL
Sbjct: 3   ASNSEAEVKKEELAETMEQ---QQLHRHNQSEVNENENENENDDESHPPLSAQSSPPHSL 62

Query: 67  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 126
           S +KDPSPPLHSPS +SDFSDHTC + GNSSID ALSIT L+DL   PS+  PSPRPVAA
Sbjct: 63  SDSKDPSPPLHSPSNASDFSDHTCQTHGNSSIDDALSITRLEDLS--PSSYPPSPRPVAA 122

Query: 127 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 186
           NRAQPSEPIVVTKVD EIQG+RKVEE   S  DVE  DGD   GG VGRGRKLM  LS+K
Sbjct: 123 NRAQPSEPIVVTKVDAEIQGVRKVEE--GSASDVEPSDGD---GGPVGRGRKLMANLSMK 182

Query: 187 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 246
           KIKREELRKKILLGFRI GF FCL S SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGF
Sbjct: 183 KIKREELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGF 242

Query: 247 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ 275
           VYSALQS+DLVYFLSTGK +IRN+FKQYFDFFIDQ
Sbjct: 243 VYSALQSFDLVYFLSTGKQMIRNYFKQYFDFFIDQ 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNE82.0e-2254.17CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
D7LIR21.0e-2134.80CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q84WP52.5e-2035.84CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
D7MMW43.1e-1531.93CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q501G61.0e-1331.78CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142358.11.85e-188100.00CASP-like protein 4A3 [Cucumis sativus] >KGN52378.1 hypothetical protein Csa_008... [more]
XP_008458753.13.64e-15588.64PREDICTED: CASP-like protein 4A1 [Cucumis melo] >KAA0037507.1 CASP-like protein ... [more]
XP_038890250.12.46e-14181.52CASP-like protein 4A1 [Benincasa hispida][more]
KAG7016184.12.15e-12876.70CASP-like protein 4A1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6578643.18.43e-12677.57CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KS018.94e-189100.00CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1[more]
A0A5A7T7H81.76e-15588.64CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00... [more]
A0A1S3C8441.76e-15588.64CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498066 PE=3 SV=1[more]
A0A6J1FJD74.12e-12477.04CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1[more]
A0A6J1JTE41.46e-12276.00CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 189..276
e-value: 1.2E-18
score: 67.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..131
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 104..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..169
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 93..275
NoneNo IPR availablePANTHERPTHR11615:SF279CASP-LIKE PROTEIN 4A1coord: 93..275

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G8305Cucsat.G8305gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8305.T4.E1Cucsat.G8305.T4.E1exon
Cucsat.G8305.T4.E2Cucsat.G8305.T4.E2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8305.T4.C1Cucsat.G8305.T4.C1CDS
Cucsat.G8305.T4.C2Cucsat.G8305.T4.C2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G8305.T4Cucsat.G8305.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0030117 membrane coat
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0005198 structural molecule activity
molecular_function GO:0008270 zinc ion binding