Cucsat.G806.T12 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G806.T12
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Enoyl-CoA hydratase
Location	ctg1: 1834152 .. 1838483 (+)
RNA-Seq Expression	Cucsat.G806.T12
Synteny	Cucsat.G806.T12

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCACGTTTTTCTATTCCTTCTTCTTTCCCTCTGCTTTGCCATCTTCCTCGTACTACCAACCAATGGGAGCTTTCAAAATCCGAAGCAGAAATCTCATCAACCTCCCAATTGAACTCCTTCATTCATCTCAGAACCTTCCCATTCCGGCCAAAAACCTCCGTCAGACTGTTCCCAGCTCCACCCCTTCCGATGCCTTCAATTTGTTCTCACATCCATGGCGATACACTCATTGCAGAACTCTCATTTTGGACTCACTTTCCACTAAGCCTGTCCGGCTTCACCGACTTTCAGACTCTGATTCTGGTAATTTCTGAGCTGAATATGTAAAATATTTTCTTTAATTTTGTTTTAATATTATAAGCTCAATGGTTAGGTCCCCATTTTGTTAATTTATGGTTTGTGATTAATGTTTAAGGCTTCATTTTGCCTTCAGAACTGATAATTCGCTATTGTTGTTTCACTGGCTACTTTCTTGATGATTTGATTGTCTGCAGAGAAGGGAAAGTGGAGACCAAAACTTTTTTTTAAACAGTAAAATTAGAGTGTTTGATAACTTCATGTCACTTTTGAAATAGTAAAAAATGGTTCAAAATTATTTTTTTAAGAAACTATTTAAAGTTGATAATTCGAAATGTGATTGATGGAAGATGACTTTGTAATTTTTCTTTTGGAAAAACAAGGAATCACTTTATGGTAATTGACATTTATAAAAGTGATTTTATTTTTAGATGTAGCGCAACCAAACATACTTACTCCTAGTATTTTTCATACATTGGGACCTCTTATAAATGAATAAAAAAAAGTCAAAATGTAATGTAACTAAACACCCAAGAATTTTCACAAGAACTATTTACTTACTCCTAGTATTTTTCATACATTGAGAACTTCCATAGTGTGACATGCACTTTCTGACAAAGTGTTCTGAGAATTTGTTAGTTCATGAATGAAGAGTACATAACCAACTACGGCTTGTTTTCTATTCTTTTTGTTCTTTTGTCCTTTTGGTTCTTGGGTCCTGTCCGATATTCTTTATGCTTGTCCTTGGATGGCTCAGACTTCCGATGGGTTCCCTTTTTCTTTATTTGTAATGTTATCTATGATCATTTCTTTTCAAGTTTCTGGGGTGTTCTTGGGCTGCTTCAGTTTTGTTTGAATGGTGATCAACTTTGGTTTATCAAACAGGAATTGTCGAGATACATTTAGACAGGCCTGAGGCGAAAAACGCTATTAGCAAGGATATGCTGAGGGGATTGCGTCATGCATTTGAATCCGTTGATAGTGATCCATCCGTCAATGTTATGATGATCCGCAGCTCTGTCCCAAAGGTGTTCTGTGCAGGTGCCGATTTGAAGGTATTCTTGTTAACTCTGCTGGTTTACATTGGAGCATTTTGGTAGAATCTCAGATAATTTGGCTCTCACAGTATGGCATGGTGTTTAACTTCCCTCAATTCATCCTTTAAGTGCAGGAACGTAAGAAGATGGCAGCATCAGAAGTTCACTCCTTTGTCACCTCACTGCGGTCTGCATTCACATTTTTAGAGGTAGTTCATTATAATCTACCTACCTACCTACTTTCTTTTACATTCTTTGATTTATATAAAGAATATTTAAACAAGGATATCTTGTCAAGGATGTAATGCAGCAGTTGATATGCACATGAGTTATCATTGATATTCTAACCGTGTTCTTTTGTTGCTAATGCTAGTAAGCTTAGTCGTCACCTAGGGTGTATGTTTTCATTCTTCAGTGGTTTGAGATTCATTTGATTCGTACTGATTCCATAAATAAAAATAGGCATTATACTTGATTTTTCAATGAACTTCAAATGGAACTTTAGGGTTTTATATACTAGTAAATGAGTGGTGAAACCTCAATTTGCTCTCAGGTTTAACCACTATGGTTTGGCCTTGTGGACAATAAGGGGTATATGTTAGTAATAAAAGGCTTAAAGGAATTCAAGCCATGGTGGCCACCTCTCACATGTTTATGCAGCTCTATGTTTCATTTATTTTTTACCTGATTAACTTAATAATTCGTCATCGTTAAGAATCCAATACTTCTCTTTCTTGAACAACTCTAGTTTTGTTTTTCACCATCTTTGAAATATTTTCACTTCGATTTTTCAGGTCCACTAGTCTCTGTCTGCTGATGTAATCTAAATTCTATTTTATTCAGGCACTTCCTATTCCTACTATTTCAGTTATTGAAGGAGCAGCATTGGGTGGTGGACTTGAAATGGCTCTTGCATGTGATCTTCGGATATGTGGTAAATAATTTATTCAATTATTTTATAGTTAAGTTTTTTACCAGACCTTCTTGCTGTATTCGCTGGCTTTATTCATGGTAGCTCACATGGTTGGCCATGATCCAGGGGAAGATGCCAAACTCAGTTTGCCAGAAACCGGACTCGCTATAATTCCTGGGTATGTAGTAAATTCTTTTTTATATATGATAGCTCACTTGAGACTAGTAGAAGGAAGCCTGCATGCAAACTGATGTTGGATAGTTCTATTACTTAGTATTAACATTAAACTATTGATGGTGGAAGGAAGCTGAATGACACTCTTCTCAAGAATATTTTTCGAATCCTCTTCCCCTTTTCTATTGATATTACCATGATGGTTTTCCAGTATGCACAAGCAGATTATAGCTTCTAATTGTGTTTTTTCACAGGGCAGGTGGGACTGCAAGGCTTCCTAGGCTGGTCGGCAAATCAATAGCAAAGGAACTTATATTCACTGGTCGTAAGGTCAGTGGTAGAGATGCTCTATCTATAGGTATGGACGCCATTAAAAAAAAAAAAATCTTCAATTCAATGTCTTATTGATCTAAGGAGAAGACAATAAAATTTCTTGCTTTAGGTCTTGTCAATTACTGCGTCTCTGCTGGGGAAGCTTATACAAAGGCACTGGAAATTGCCCAAGAGATAAATGAGAAGGTAATTAAAACATCTAATATAATTATCTTTCTGGGTATCATGCCACAATTTGATGAAAATTATGATTTGATTGATATGATACCACTAATTCTAGGACGACCATCTGATGGATAAAGAGATCAACGTTGTATCCTATTCATTGTTTCAGACATCGTGGAGTTTTTTTTTTAAGTGAATCATATTTTGCACCTTAAGTTCCATCCTTCAACTGAGAAGTGTTATAGAACGATTCTTAAACCCACTAACTAAACACTATCATTTTTAGTTTAATCTGTGAAGTGACATCTACACTAACACTTTTCAATAAGAGAACTGTGTTCAAATGGGGAAATAATTTAATCTCCTCCAAATTTGTTACCATCGCTAGTGAAATAGTGAATGGTTGATCAATTTGAATCATTTCTTAAATTTCAAGCGTTATAATGTAACTATACATCAAAATGAACCATTTTTAGTAATTATGTAGATAGAAATATAGGCTTCTAACTTCTTGTTTTCTGAACTGTCAGTTTCTGTTTACGTGTTGATATTAGTATATACCTTGGCTCACGAGTTAGCCAAAAGGGTTTTTTCTGGCATTTCTTTTACTTGGATGTTTAATTTTCCTGATTGGTTATTCTATCATTGTAGTCATGATGTAAGAAACTTTATCCAGCTACAGCGAGTTGGTAGTGGTAATTTTATTTTTATTGCTTTTAATAATAATGATAAAAAGGAAAAAGAGTAGAAATATATTCTTCCTTAACTCTGTCATGTTGTGCATACCGCCAACAATCTAATGCCCAAGTAATTGTTGAGTATTGTAACAGGGTCCACTAGCAATAAGGATGGCAAAGAAGGCGATCAACGAGGGGCTCGAGGGAGACCTGGAGTCAGCTATGGAAATAGAAGATGAGTGTTACACAGAACTCTTGGACACGAAAGACCGGTTGGAAGGATTGGCAGCATTTGCCGAGAAGCGAAAGCCAAGGTATAGAGGAGAATAGATTGATAACCACTTGCTTATTTTCACAATACAATGTGGTTACCTCTAATCTTCATTTTAACTTTCTGAAGTATAAGAATAAATGCAAGGTTTGCATTAAGCATAGTTACCTAAAATGAAAACTGTACCAAATTCAGCTTATTTGATCAGTGATGCTATAATAAAGCAGCCTTGAATGCCTTAGTGTTTGCACTCATTGATCTCTGTCCTGAAATTTAGGATTGCTCTTTGTTACTTTTAGTTTGCCAGTTGGGTGATTAGGAGTGTAAAAATGGTTAGGGTTGTCATTAACCAAACAACTTGATTGAGTTGTGAATGATGTGAATGATGTGAATGTAACCTTTTATTATTCAAGTAACTATGGTATTACATTTTCATTGTTATTTTCACATTTTGTACCATTTCA

Coding sequence (CDS)

CTCACGTTTTTCTATTCCTTCTTCTTTCCCTCTGCTTTGCCATCTTCCTCGTACTACCAACCAATGGGAGCTTTCAAAATCCGAAGCAGAAATCTCATCAACCTCCCAATTGAACTCCTTCATTCATCTCAGAACCTTCCCATTCCGGCCAAAAACCTCCGTCAGACTGTTCCCAGCTCCACCCCTTCCGATGCCTTCAATTTGTTCTCACATCCATGGCGATACACTCATTGCAGAACTCTCATTTTGGACTCACTTTCCACTAAGCCTGTCCGGCTTCACCGACTTTCAGACTCTGATTCTGGAATTGTCGAGATACATTTAGACAGGCCTGAGGCGAAAAACGCTATTAGCAAGGATATGCTGAGGGGATTGCGTCATGCATTTGAATCCGTTGATAGTGATCCATCCGTCAATGTTATGATGATCCGCAGCTCTGTCCCAAAGGTGTTCTGTGCAGGTGCCGATTTGAAGGAACGTAAGAAGATGGCAGCATCAGAAGTTCACTCCTTTGTCACCTCACTGCGGTCTGCATTCACATTTTTAGAGGCACTTCCTATTCCTACTATTTCAGTTATTGAAGGAGCAGCATTGGGTGGTGGACTTGAAATGGCTCTTGCATGTGATCTTCGGATATGTGGGGAAGATGCCAAACTCAGTTTGCCAGAAACCGGACTCGCTATAATTCCTGGGGCAGGTGGGACTGCAAGGCTTCCTAGGCTGGTCGGCAAATCAATAGCAAAGGAACTTATATTCACTGGTCGTAAGGTCAGTGGTAGAGATGCTCTATCTATAGGTCTTGTCAATTACTGCGTCTCTGCTGGGGAAGCTTATACAAAGGCACTGGAAATTGCCCAAGAGATAAATGAGAAGTCATGA

Protein sequence

LTFFYSFFFPSALPSSSYYQPMGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTLILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVSAGEAYTKALEIAQEINEKS

Homology

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot
Match: F4JML5 (Probable enoyl-CoA hydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g16800 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 3.8e-77
Identity = 145/214 (67.76%), Postives = 180/214 (84.11%), Query Frame = 0


Query: 78  CRTLILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPS 137
           CRTLI+++   + V+L+RLS SDSGI+E++LDRP  KNAI+K+ML+ L++AFES+  D S
Sbjct: 30  CRTLIIETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNS 89

Query: 138 VNVMMIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAA 197
             V+MIRS VP VFCAGADLKER+ M+ SEVH++V SLR  F+F+EAL IPTI+ IEGAA
Sbjct: 90  ARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 149

Query: 198 LGGGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKV 257
           LGGGLEMALACDLRICGE+A   LPETGLAIIPGAGGT RL RLVG+S++KELIFTGRK+
Sbjct: 150 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 209

Query: 258 SGRDALSIGLVNYCVSAGEAYTKALEIAQEINEK 292
              +A + GLVN CV+AGEA+ KA+E+AQ+INEK
Sbjct: 210 DAIEAANKGLVNICVTAGEAHEKAIEMAQQINEK 243

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot
Match: Q9JLZ3 (Methylglutaconyl-CoA hydratase, mitochondrial OS=Mus musculus OX=10090 GN=Auh PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.6e-49
Identity = 99/201 (49.25%), Postives = 143/201 (71.14%), Query Frame = 0


Query: 91  VRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKV 150
           +R+  L + + GIV + ++R   KNA+SK++L+ L  A +++ SD  V  ++IRS VP +
Sbjct: 52  LRVRHLEEENRGIVVLGINRAYGKNALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGI 111

Query: 151 FCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDL 210
           FCAGADLKER KM +SEV  FV+ +RS    +  LP+PTI+ I+G ALGGGLE+ALACD+
Sbjct: 112 FCAGADLKERAKMHSSEVGPFVSKIRSVINDIANLPVPTIAAIDGLALGGGLELALACDI 171

Query: 211 RICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNY 270
           R+    AK+ L ET LAIIPG GGT RLPR +G S+AKELIF+ R + G++A ++GL+++
Sbjct: 172 RVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISH 231

Query: 271 CVSAGE----AYTKALEIAQE 288
            +   +    AY KAL++A+E
Sbjct: 232 VLEQNQEGDAAYRKALDLARE 252

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot
Match: Q13825 (Methylglutaconyl-CoA hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=AUH PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 7.5e-49
Identity = 96/201 (47.76%), Postives = 144/201 (71.64%), Query Frame = 0


Query: 91  VRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKV 150
           +R+  L + + GIV + ++R   KN++SK++++ L  A +++ SD  V  ++IRS VP +
Sbjct: 77  LRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGI 136

Query: 151 FCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDL 210
           FCAGADLKER KM++SEV  FV+ +R+    +  LP+PTI+ I+G ALGGGLE+ALACD+
Sbjct: 137 FCAGADLKERAKMSSSEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDI 196

Query: 211 RICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNY 270
           R+    AK+ L ET LAIIPG GGT RLPR +G S+AKELIF+ R + G++A ++GL+++
Sbjct: 197 RVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISH 256

Query: 271 CVSAGE----AYTKALEIAQE 288
            +   +    AY KAL++A+E
Sbjct: 257 VLEQNQEGDAAYRKALDLARE 277

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot
Match: Q54HG7 (Methylglutaconyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=auh PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.3e-48
Identity = 104/199 (52.26%), Postives = 131/199 (65.83%), Query Frame = 0


Query: 93  LHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFC 152
           L RL   + GI  I  +R   KNA+ K+++   R     +   P   V+++RS V  VFC
Sbjct: 47  LERLEGENKGISVISFNRGHVKNALGKNLMNQFRSHLNELRFCPDTRVVIVRSLVDGVFC 106

Query: 153 AGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDLRI 212
           +GADLKER  M+  E   FV SLRS+FT LE L +PTI+ IEG A+GGG EM LACD R+
Sbjct: 107 SGADLKERALMSQVEASQFVHSLRSSFTELETLQMPTIAAIEGVAVGGGTEMVLACDFRV 166

Query: 213 CGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCV 272
             + +K+ LPETGLAIIPGAGGT RLPRL+G   AKELIFTG  +  + AL IGLV Y  
Sbjct: 167 ASKSSKMGLPETGLAIIPGAGGTQRLPRLIGIPRAKELIFTGAILDSKRALEIGLVQYET 226

Query: 273 SAGEAYTKALEIAQEINEK 292
             GEA+ KA+EIA++I  K
Sbjct: 227 EKGEAFDKAIEIAKQIIPK 245

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot
Match: F1LU71 (Methylglutaconyl-CoA hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Auh PE=1 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 2.8e-48
Identity = 96/201 (47.76%), Postives = 143/201 (71.14%), Query Frame = 0


Query: 91  VRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKV 150
           +R+  L + + GIV + ++R   KN++SK++L+ L  A +++ SD  V  ++IRS VP +
Sbjct: 53  LRVRHLEEENRGIVVLGINRAYGKNSLSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGI 112

Query: 151 FCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDL 210
           FCAGADLKER KM +SEV  FV+ +R+    +  LP+PTI+ I+G ALGGGLE+ALACD+
Sbjct: 113 FCAGADLKERAKMHSSEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALACDI 172

Query: 211 RICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNY 270
           R+    AK+ L ET LAIIPG GGT RLPR +G ++AKELIF+ R + G++A ++GL+++
Sbjct: 173 RVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMALAKELIFSARVLDGQEAKAVGLISH 232

Query: 271 CVSAGE----AYTKALEIAQE 288
            +   +    AY KAL++A+E
Sbjct: 233 VLEQNQEGDAAYRKALDLARE 253

[Close]

BLAST of Cucsat.G806.T12 vs. NCBI nr
Match: XP_031739486.1 (probable enoyl-CoA hydratase 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 530 bits (1366), Expect = 1.69e-188
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL
Sbjct: 1   MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCVSAGEAYTKALEIAQEINEK
Sbjct: 241 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 270

[Close]

BLAST of Cucsat.G806.T12 vs. NCBI nr
Match: XP_008448801.1 (PREDICTED: probable enoyl-CoA hydratase 2, mitochondrial isoform X2 [Cucumis melo] >KAA0041061.1 putative enoyl-CoA hydratase 2 [Cucumis melo var. makuwa] >TYK12047.1 putative enoyl-CoA hydratase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 498 bits (1282), Expect = 1.05e-175
Identity = 254/270 (94.07%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIR RNLINL I+L  S QNLPIPA NLRQT PSSTPSDAFN FSHPWRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCV AGEAY KALEIAQEIN+K
Sbjct: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQK 270

[Close]

BLAST of Cucsat.G806.T12 vs. NCBI nr
Match: XP_008448800.1 (PREDICTED: probable enoyl-CoA hydratase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 491 bits (1263), Expect = 1.10e-172
Identity = 254/278 (91.37%), Postives = 257/278 (92.45%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIR RNLINL I+L  S QNLPIPA NLRQT PSSTPSDAFN FSHPWRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRIC--------GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 261
           LEMALACDLRIC        GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT
Sbjct: 181 LEMALACDLRICAHMVGHDLGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240

Query: 262 GRKVSGRDALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           GRKVSGRDALSIGLVNYCV AGEAY KALEIAQEIN+K
Sbjct: 241 GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQK 278

[Close]

BLAST of Cucsat.G806.T12 vs. NCBI nr
Match: XP_038905583.1 (probable enoyl-CoA hydratase 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 483 bits (1243), Expect = 9.15e-170
Identity = 246/270 (91.11%), Postives = 256/270 (94.81%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKI SRNLINL I+L HS QNLPIPA  LRQ +P+S+ SD FN FSHPWRYTHCRTL
Sbjct: 1   MGAFKILSRNLINLHIQLHHSPQNLPIPAGILRQALPTSSSSDGFNSFSHPWRYTHCRTL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           IL+SLS++PVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFES+ SDPSVNVM
Sbjct: 61  ILESLSSEPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESIYSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCV AGEAYTKALEIAQEIN+K
Sbjct: 241 ALSIGLVNYCVPAGEAYTKALEIAQEINQK 270

[Close]

BLAST of Cucsat.G806.T12 vs. NCBI nr
Match: XP_023005458.1 (probable enoyl-CoA hydratase 2, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 446 bits (1148), Expect = 2.37e-155
Identity = 229/270 (84.81%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAF++ SR LINL     HS QNLPIPAK+L    P+S  SD F  FSHPW+Y+HCRTL
Sbjct: 1   MGAFRVLSRKLINLS---RHSLQNLPIPAKHLAHPFPTSNLSDGFKSFSHPWQYSHCRTL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           IL+S+S++PVRLHRLSDSDSGIVE+HLDRPEAKNAISK+MLRGL HAFESVD DPSVNVM
Sbjct: 61  ILESVSSEPVRLHRLSDSDSGIVEVHLDRPEAKNAISKEMLRGLCHAFESVDCDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MIRSSVPKVFCAGADLKERK M ASEV SFVTSLRSAF+FLEAL IPTISVIEGAALGGG
Sbjct: 121 MIRSSVPKVFCAGADLKERKNMTASEVQSFVTSLRSAFSFLEALRIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           A+SIGLVNYCV AG AY KALEIAQEIN+K
Sbjct: 241 AISIGLVNYCVPAGGAYVKALEIAQEINQK 267

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL
Match: A0A0A0L6S6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G017300 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 8.20e-189
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL
Sbjct: 1   MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCVSAGEAYTKALEIAQEINEK
Sbjct: 241 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 270

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL
Match: A0A5A7THC3 (Putative enoyl-CoA hydratase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00600 PE=3 SV=1)

HSP 1 Score: 498 bits (1282), Expect = 5.11e-176
Identity = 254/270 (94.07%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIR RNLINL I+L  S QNLPIPA NLRQT PSSTPSDAFN FSHPWRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCV AGEAY KALEIAQEIN+K
Sbjct: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQK 270

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL
Match: A0A1S3BLF9 (probable enoyl-CoA hydratase 2, mitochondrial isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490860 PE=3 SV=1)

HSP 1 Score: 498 bits (1282), Expect = 5.11e-176
Identity = 254/270 (94.07%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIR RNLINL I+L  S QNLPIPA NLRQT PSSTPSDAFN FSHPWRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           ALSIGLVNYCV AGEAY KALEIAQEIN+K
Sbjct: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQK 270

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL
Match: A0A1S3BL64 (probable enoyl-CoA hydratase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490860 PE=3 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 5.33e-173
Identity = 254/278 (91.37%), Postives = 257/278 (92.45%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAFKIR RNLINL I+L  S QNLPIPA NLRQT PSSTPSDAFN FSHPWRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           ILDSLSTKPVRLHRLSDSDSGIVE+HLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MI SSVPKVFCAGADLKERKKM ASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRIC--------GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 261
           LEMALACDLRIC        GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT
Sbjct: 181 LEMALACDLRICAHMVGHDLGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240

Query: 262 GRKVSGRDALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           GRKVSGRDALSIGLVNYCV AGEAY KALEIAQEIN+K
Sbjct: 241 GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQK 278

[Close]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL
Match: A0A6J1L289 (probable enoyl-CoA hydratase 2, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498438 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 1.15e-155
Identity = 229/270 (84.81%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 22  MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 81
           MGAF++ SR LINL     HS QNLPIPAK+L    P+S  SD F  FSHPW+Y+HCRTL
Sbjct: 1   MGAFRVLSRKLINLS---RHSLQNLPIPAKHLAHPFPTSNLSDGFKSFSHPWQYSHCRTL 60

Query: 82  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 141
           IL+S+S++PVRLHRLSDSDSGIVE+HLDRPEAKNAISK+MLRGL HAFESVD DPSVNVM
Sbjct: 61  ILESVSSEPVRLHRLSDSDSGIVEVHLDRPEAKNAISKEMLRGLCHAFESVDCDPSVNVM 120

Query: 142 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 201
           MIRSSVPKVFCAGADLKERK M ASEV SFVTSLRSAF+FLEAL IPTISVIEGAALGGG
Sbjct: 121 MIRSSVPKVFCAGADLKERKNMTASEVQSFVTSLRSAFSFLEALRIPTISVIEGAALGGG 180

Query: 202 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 261
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 262 ALSIGLVNYCVSAGEAYTKALEIAQEINEK 291
           A+SIGLVNYCV AG AY KALEIAQEIN+K
Sbjct: 241 AISIGLVNYCVPAGGAYVKALEIAQEINQK 267

[Close]

BLAST of Cucsat.G806.T12 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 289.7 bits (740), Expect = 2.7e-78
Identity = 145/214 (67.76%), Postives = 180/214 (84.11%), Query Frame = 0


Query: 78  CRTLILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPS 137
           CRTLI+++   + V+L+RLS SDSGI+E++LDRP  KNAI+K+ML+ L++AFES+  D S
Sbjct: 30  CRTLIIETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNS 89

Query: 138 VNVMMIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAA 197
             V+MIRS VP VFCAGADLKER+ M+ SEVH++V SLR  F+F+EAL IPTI+ IEGAA
Sbjct: 90  ARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 149

Query: 198 LGGGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKV 257
           LGGGLEMALACDLRICGE+A   LPETGLAIIPGAGGT RL RLVG+S++KELIFTGRK+
Sbjct: 150 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 209

Query: 258 SGRDALSIGLVNYCVSAGEAYTKALEIAQEINEK 292
              +A + GLVN CV+AGEA+ KA+E+AQ+INEK
Sbjct: 210 DAIEAANKGLVNICVTAGEAHEKAIEMAQQINEK 243

[Close]

BLAST of Cucsat.G806.T12 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 112.8 bits (281), Expect = 4.5e-25
Identity = 70/208 (33.65%), Postives = 109/208 (52.40%), Query Frame = 0


Query: 98  DSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFCAGADL 157
           ++DS +  + ++RP   NA+S D       A  S+D +P V+V+++ S   K FC+G DL
Sbjct: 15  NTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL-SGAGKHFCSGIDL 74

Query: 158 KERKKM------------AASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMA 217
                +            ++ ++   + S+++A T +E    P I+ I GA +GGG+++ 
Sbjct: 75  NSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHGACIGGGVDLI 134

Query: 218 LACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSI 277
            ACD+R C EDA  S+ E  LAI+   G   RLP +VG + A EL  T R+ SG +A  +
Sbjct: 135 TACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGSEAKDL 194

Query: 278 GLVNYCV-SAGEAYTKALEIAQEINEKS 293
           GLV+    S  E       IA+ I  KS
Sbjct: 195 GLVSKVFGSKSELDNGVTTIAEGIGGKS 221

[Close]

BLAST of Cucsat.G806.T12 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 112.5 bits (280), Expect = 5.9e-25
Identity = 63/184 (34.24%), Postives = 101/184 (54.89%), Query Frame = 0


Query: 98  DSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFCAGADL 157
           ++DS +  + ++RP   NA+S D       A  S+D +P V+V+++ S   K FC+G DL
Sbjct: 15  NTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL-SGAGKHFCSGIDL 74

Query: 158 KERKKM------------AASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMA 217
                +            ++ ++   + S+++A T +E    P I+ I GA +GGG+++ 
Sbjct: 75  NSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHGACIGGGVDLI 134

Query: 218 LACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSI 270
            ACD+R C EDA  S+ E  LAI+   G   RLP +VG + A EL  T R+ SG +A  +
Sbjct: 135 TACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGSEAKDL 194

[Close]

BLAST of Cucsat.G806.T12 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 105.9 bits (263), Expect = 5.5e-23
Identity = 71/190 (37.37%), Postives = 101/190 (53.16%), Query Frame = 0


Query: 102 GIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFCAGADLK--- 161
           G+  I L  P   N++S D+L  L+  +E   S   V  ++I  +  + F  G D+    
Sbjct: 17  GVAVITLINPPV-NSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGR-FSGGFDISGFG 76

Query: 162 ERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDLRICGEDAK 221
           E +K    E  +   S+      LEA   P+++ I+G ALGGGLE+A+AC  RI    A+
Sbjct: 77  EMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARISAPAAQ 136

Query: 222 LSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVSAGEAY 281
           L LPE  L +IPG GGT RLPRLVG + A E+I T + V   +  S+GL++  V   E  
Sbjct: 137 LGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHSLGLIDAVVPPAELV 196

Query: 282 TKALEIAQEI 289
           T A   A +I
Sbjct: 197 TTARRWALDI 204

[Close]

BLAST of Cucsat.G806.T12 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-21
Identity = 64/196 (32.65%), Postives = 101/196 (51.53%), Query Frame = 0


Query: 93  LHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVMMIRSSVPKVFC 152
           L ++     GI  I ++RP++ N++++ M+  L  AF+ +DSD SV V++   S  + FC
Sbjct: 9   LIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGS-GRSFC 68

Query: 153 AGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGGLEMALACDLRI 212
           +G DL   + +   +V    T        +E L  P I  I G A+  G E+ALACD+ +
Sbjct: 69  SGVDLTAAESVFKGDVKDPET---DPVVQMERLRKPIIGAINGFAITAGFELALACDILV 128

Query: 213 CGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCV 272
               AK         I P  G + +L R++G + A+E+  T   ++   A  +G VN+ V
Sbjct: 129 ASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVNHVV 188

Query: 273 SAGEAYTKALEIAQEI 289
             GEA  KA EIA+ I
Sbjct: 189 EEGEALKKAREIAEAI 200

The following BLAST results are available for this feature:

BLAST of Cucsat.G806.T12 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JML5	3.8e-77	67.76	Probable enoyl-CoA hydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9JLZ3	2.6e-49	49.25	Methylglutaconyl-CoA hydratase, mitochondrial OS=Mus musculus OX=10090 GN=Auh PE...	[more]
Q13825	7.5e-49	47.76	Methylglutaconyl-CoA hydratase, mitochondrial OS=Homo sapiens OX=9606 GN=AUH PE=...	[more]
Q54HG7	1.3e-48	52.26	Methylglutaconyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=446...	[more]
F1LU71	2.8e-48	47.76	Methylglutaconyl-CoA hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=A...	[more]

BLAST of Cucsat.G806.T12 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739486.1	1.69e-188	100.00	probable enoyl-CoA hydratase 2, mitochondrial [Cucumis sativus]	[more]
XP_008448801.1	1.05e-175	94.07	PREDICTED: probable enoyl-CoA hydratase 2, mitochondrial isoform X2 [Cucumis mel...	[more]
XP_008448800.1	1.10e-172	91.37	PREDICTED: probable enoyl-CoA hydratase 2, mitochondrial isoform X1 [Cucumis mel...	[more]
XP_038905583.1	9.15e-170	91.11	probable enoyl-CoA hydratase 2, mitochondrial isoform X1 [Benincasa hispida]	[more]
XP_023005458.1	2.37e-155	84.81	probable enoyl-CoA hydratase 2, mitochondrial isoform X2 [Cucurbita maxima]	[more]

BLAST of Cucsat.G806.T12 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6S6	8.20e-189	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G017300 PE=3 SV=1	[more]
A0A5A7THC3	5.11e-176	94.07	Putative enoyl-CoA hydratase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BLF9	5.11e-176	94.07	probable enoyl-CoA hydratase 2, mitochondrial isoform X2 OS=Cucumis melo OX=3656...	[more]
A0A1S3BL64	5.33e-173	91.37	probable enoyl-CoA hydratase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656...	[more]
A0A6J1L289	1.15e-155	84.81	probable enoyl-CoA hydratase 2, mitochondrial isoform X2 OS=Cucurbita maxima OX=...	[more]

BLAST of Cucsat.G806.T12 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16800.1	2.7e-78	67.76	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT5G43280.1	4.5e-25	33.65	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT5G43280.2	5.9e-25	34.24	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT3G06860.1	5.5e-23	37.37	multifunctional protein 2	[more]
AT4G16210.1	1.8e-21	32.65	enoyl-CoA hydratase/isomerase A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 281..292
None	No IPR available	GENE3D	3.90.226.10		coord: 78..292 e-value: 3.6E-73 score: 248.4
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 75..292
None	No IPR available	PANTHER	PTHR11941:SF105	FI23914P1-RELATED	coord: 75..292
None	No IPR available	CDD	cd06558	crotonase-like	coord: 91..288 e-value: 1.05831E-76 score: 230.14
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 100..292 e-value: 7.6E-52 score: 176.1
IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	PROSITE	PS00166	ENOYL_COA_HYDRATASE	coord: 190..210
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 88..292

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G806	Cucsat.G806	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G806.T12.E1	Cucsat.G806.T12.E1	exon
Cucsat.G806.T12.E2	Cucsat.G806.T12.E2	exon
Cucsat.G806.T12.E3	Cucsat.G806.T12.E3	exon
Cucsat.G806.T12.E4	Cucsat.G806.T12.E4	exon
Cucsat.G806.T12.E5	Cucsat.G806.T12.E5	exon
Cucsat.G806.T12.E6	Cucsat.G806.T12.E6	exon
Cucsat.G806.T12.E7	Cucsat.G806.T12.E7	exon
Cucsat.G806.T12.E8	Cucsat.G806.T12.E8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G806.T12.C1	Cucsat.G806.T12.C1	CDS
Cucsat.G806.T12.C2	Cucsat.G806.T12.C2	CDS
Cucsat.G806.T12.C3	Cucsat.G806.T12.C3	CDS
Cucsat.G806.T12.C4	Cucsat.G806.T12.C4	CDS
Cucsat.G806.T12.C5	Cucsat.G806.T12.C5	CDS
Cucsat.G806.T12.C6	Cucsat.G806.T12.C6	CDS
Cucsat.G806.T12.C7	Cucsat.G806.T12.C7	CDS
Cucsat.G806.T12.C8	Cucsat.G806.T12.C8	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G806.T12	Cucsat.G806.T12-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044281	small molecule metabolic process
molecular_function	GO:0003824	catalytic activity