Cucsat.G7523.T5 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G7523.T5
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Regulator of Vps4 activity in the MVB pathway protein
Location	ctg1528: 4047857 .. 4052980 (+)
RNA-Seq Expression	Cucsat.G7523.T5
Synteny	Cucsat.G7523.T5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAACTCGTAAAGTTGATGATCGGTACACGGAAAGGAAGTCCTTTTTCAGTTGCTCTTTCTTCGCTCCGACTTCGTTTCCTATTCATTCTTCTTCATTACTATATCTTCTTCAATCATCCTAATTCATTCATTCATTCTTCTTCTTAATCTTCACGGTTCCATTTCACATCCTTTCTTCAAAGGATTTTTGTTAGGAAAAAAGAGTTTCGGTGCGTAATTGTCCCGCCAGCTTAACCTTTGTTGTTTGTTCCCACCGCGTTGAGATCTGGTTTCTTTCGATTCCTTCTACGATGCTAAGCAAGAGCTTCAAGCCTGCGAAATGGTAGGTTTGAGATTTCTTCAACTTAATCCTTGTAGTTTTTGTTCTGTTGTTGGGTACTTTTGGCTGGGATCAATTTATTGACTTTTTTTTTTTTGCATTTTTTGTTTGAATTGGGTTGGGTCGGTGCAGTAAGACATCGCTGAAGTTAGCGGTTTCGCGGATTAAGCTTTTGAGGAATAAGAAAGATGTTCATATTAAGCAGCTTAAGGGAGATTTGGCTAAGTTGCTTGAGGCTGGACAAGACCAGACTGCTAGAATTCGGGTATTTTAGTGTTGTTCTTTGACTCTGGAATTGATTGTATTTATTTGTGTCTTTATCAAGTCACCGTGTTTTTTAGACCTAATGTCATATGGGAAGATGAACTTTATCGTGCGTCAATTTGATGATTCCTTTTCCGAAGAATTTACGTTTCCATCTTGTTTCAGGTTGAACACTTTGTTAGAGAAGAGAAATCGAAGGAAGCTTATGAACTCATTGAAATATTTTGTGAGCTAATTGTGGCGCGCATGCCAATGATTGAGTCCCAAAAGTATGTTAATTTCTTTTTTATTGAAGTTATTTTTGTTGATTTCTCATGAACTTCGCTGCCTATAGGGTATGCTTTGATTTCAGAATGTGCATTTGTATTCGACTTCACATGGTTGTGATTTCTTTTAAAGCAAAATTTTGCCCTCGTGACGTATGTGTTAACTTCTGATTTCTGAGCATAATGAATGTACTCCTATTAATTCGGTTTATGTTTTCATGTTCAGAAATTGTCCCATAGATTTGAAGGAAGCAGTCTCGAGTGTTATATTTGCATCACCCAGATGTGCAGATATTCCAGAGCTCTTGGATGTTCGTAAACACTTTAAATCAAAGTATGGAAAAGAATTTGTCTCGGCCGCAGTGGAGCTGCGCCCAGAATGTGGAGTGAATCGCATGGTAAATGTGTTAGCTGTTTTGTGTTCATAAACTTAGGGAGTGTGGGTTCTCTCTATGAAGATAACTTGGTTCCATTGACTTTCCTTCCATTCTTACAACTTTCCAGTTAGTCGAAAAACTATCTGCTAAAGCACCGGATGGGCAGACCAAACTTAAAATCTTGACTGCAATTGCTGAGGAGTATAACATCAAATGGGACCCAAAATCCTTTGGTGACAGCATCAACCCTCCGGCTGATTTGTTGGTAATTTTAGTTTTTATAAAAAAGAACTATTTCCTTCAATGCTCAAGGTGTTTTTATCAGGAAATGAAGACTTACCTGAATTATTTTATTTTAATATTGAATTTCGGTTTTAGTAATACTAGTTATGAACTTTGTTGAGCAGAGTGGACCAAATACTTTTGGGAAGGCTAGTCAAATACAGATGGAGTCTATTAGTGGCCCATCTTCATTTGATCACAAAGAATCTTCTAGAAAACATGTTCCATTCAAACCTGATGAAAGGCCTCATGTACCCGAGAGATCCCCTGAACACAGTTTGAGATCAGAACATCAGTCCAAACAATCCAACTTTGCTCACGTTAATGCCAATCAAAGCAACATCACTGGCCGTCATAATTCAGAAACATCTTTTGGTATGTGAAAATTATATCTGATGCAATCATCTTCATTTCTTTCTTCTCTATGTACGTGCTACACATACATATTGAAGACAGTTACTTTTGTTTGAATTTGCAATTGTTGGTTATATATTGAAACTGTGATATGCGTAAATTAATGCAGAGGGGATGCATAGGCACTCAAATTCTGGTGAACAAAATAATTACTCTTCAGGTAGGCAGCAGTGGAGTATGGATTTTAAGGATGCTACCTCTGCTGCAAAGGCAGCGGCTGAATCTGCAGAACTAGCAAGTCTAGCTGCCCGAGCTGCTGCAGAACTTTCCAGTCGTGGAAACATCTCCCAACCATCCTCTTCTGAGTTTCAGAAATCTTCGTCTTATAATTTAAGGGCAGAAGGACCTCAAGGATACCCTAGTGTCCATTTACGTGATCAACAGCTTCCTAAAGACCAAGTTGTCAGTGCACCTCGTAAAAGTTCTACGCCGGATGATAATTGGAGAGACAATGAGACAAGAACATACATGGGGGATAATTCCAAGAATTATAGTTATCCATCTTCAAGCGTTAGTAACAATGATGTCAACATATCTGTGACCAATCTCAGTGCAGCAGAAAGATCCTCTTTCAAGAAATCATCCGAACCTAGGTTTAGCGGTTCCCTTGGTAGTAGTGCAACTTTGGAGAAACAAACCAGAAAACATGATGCCAGCACGTCTGTAACCAGTTTCAATGCTGCAGATAGATACTCTTTCAAGAATTCATTCGAACCTGGGGTCAGTGGCTCTCTTGATAGTGGGGATGAACAACCCAGAAATTTTAGTTCCAACATATCTTCCACTAATTTCAACGAATCAGACACATACTCGCTCAACAAGCCGTCTGAATCTGGGTTTAGTGACTCTTTAGGTAGAACCAGTATGGAGAAACAACCCCGAAATGTTGATGTAGAATATGTTAGCGATCAGCCATTTAGTACGGGTTTTGACAGAACTAGTTCCTATGGAGATGTGAGGATTGAAAGTGATTCAATTAAAGTGCCTTCCCACGAGAAGTTGGGGAATGATGCCTATGAGAACCCTTTTGCCATGGATAAACCAAATGAGAGTGAAAGTACTGTTGATATGAGTTTTTATGACCATGCCAGTGTGGTCTTTGATGATTATGGTCCAGATGATGATTATATTCCAGACTACGATATCCCGAGGAGAGAATCAATTCCAGACCTTTCGTCACCTAAAGGAAAGGTGCCAATAAATCCATCACCAGATGATACTTGGATCTTCAACGGGAACAAGAATGATTCTGCTGAGAAGGCTGTTTCACACGCTCAAATTTCTGATCATACTTCACTGTTTGCTGAAAGTATTGGAGCTTTTGATGATCCTTCCCATTCTGATGAGTTGTTGCCGGCAACTTTTGACCATTCAGATGGATCTGGTTCTGAAAGTGAGGAAGAGTTGAAAGAATCTGAGATCATTGCTAAAGAAAACTCTAGTGAATTCTGCAAGAAGCAGGATTTGTACTCTGAAAAATCAGAATGGACTCGGAACATTAGTCATGGATTATCTGGATCTTCAGATGAGGACAGTAGTAGCATGCCATCTCATCGCCTCTCTTCTGAGCTAAACTCTGTACATGAATCGAAGAAGAACGACAGCCCACTGAGTTCTCCTGATATTGTAGAAGAATCAACTTCAGATGGCAGCAGTGGCTTGAATTTCGGGAAACTGAAAGGTGGCCGAAGAAACCAAAAAAGTAATAAGCTTCCATTTGCAAACAATTCATCAAGAAATGACTCATCATCTAAACAAGCATACGAAAACGATGCCTCAAAAACTGAGCAGTCTACCTTCATTTCTTCTTCTACAGCTAGGACTTCGTTGCGATCAAAGGCTTCTGAAGAGACATATGCTACAAGTGTAGAAGAGAGACGAGGTCAAGAAAAGGAATCACAAACCAAGCTTAATAGCTTTAATTCCAATCTTGATGATTCAAAGGAGAAGTTCTCGGTTTATACTCTTAGAAGTGATCAAGAGCCACATAGCAAGAACGTGGTTGATGAAATCTTGAAGAATCCAGCCCCAACAAGAGTTGCAGTTAAGTATCCTGGTTTCCACAATGACGATGATTCTGACGAAGATTTGCCTGAACAAAATATGAAAAACAGTCCTCATCGAGTTATTGGACTTTCTAGACGGACCAAAGCGTCTCCTAAAAGTCCAAGTCCACATTTAGAGGACTCACACAGGACTCCCACGACCATGAGTCATGAGGATATAATTGAGAGAAAAGCTTCAACGAGTTTCTATGCAACTACATCGCCACTGAGGGCAAAAACTGGAACAAGATACTCTGATCGTTTGGAAATTTCAGAGCAGCCTCAATCATCTAAACCTTTCAAGCAAACTCATGAAACTAAGAGGTCTTTTATTGAGGAAAGGTCTAGACCTTCTGCAGAGGAACAGCAATACAATTATCCACCAGAGATAAATAGACGAGGCAATTTTGAGAGTTCAAAGTTTTCTTCTTCAAGGGATACGACAGCAGCTCCAGTGAAGACTCGGGTTCAATCAAGTAACTCAGAGCAACCTCAGTCAATGAAACCTTCCAAACCAATTCCTGAAACTAAAAAATCTATCCATGAAGAAAAATTAAAATCTCCTACGAAGGACTTGCCATCCACTCCTTCACCAAAATTAGAAACACAAGGCAACTCTGAGAGTTCGAAAAAGGAGAAGACTGAGGCAGTTGAGAAAGCTAGTCATGTTCACCCAAAGCTACCCGACTACGACAACTTTGCAGCTCACTTCCTCGCTCTTAGACAGAATAACAAGTAAATCTAGTTTCTTGGCGTTGATTTATACTTATACAGTTATACATACGACATAAATATATTTTTTGATTTATTAATTTAATTAGTCCATGTTCATGGTTATTTATTAGATAGCTCTTATTCATGATTCGAACTCTAAAGGATACATCAGATGTACAGGTTAGATTAATGGTTTGTTGTTTAGGAAAAAGCAATCAGAAGCTAATTGTGGAAATCACATAAAAGCTTTCCCAATTAATTAGCCTAAAGGTCAGATAGGCCATAATGTGTAAATGGAGAAAGGTATCTACACCACAATAAAGCTAGAGAAAGAACCAATTCTTAGAATCTACCACTAAAAAGGAGATATGTTGTTGAAAATCTGATTGTACCTTTCAGTCCATAATGACCAGAAAAAAAAAAAGGCTCTGACAG

Coding sequence (CDS)

ATGCTAAGCAAGAGCTTCAAGCCTGCGAAATGTAAGACATCGCTGAAGTTAGCGGTTTCGCGGATTAAGCTTTTGAGGAATAAGAAAGATGTTCATATTAAGCAGCTTAAGGGAGATTTGGCTAAGTTGCTTGAGGCTGGACAAGACCAGACTGCTAGAATTCGGGTTGAACACTTTGTTAGAGAAGAGAAATCGAAGGAAGCTTATGAACTCATTGAAATATTTTGTGAGCTAATTGTGGCGCGCATGCCAATGATTGAGTCCCAAAAAAATTGTCCCATAGATTTGAAGGAAGCAGTCTCGAGTGTTATATTTGCATCACCCAGATGTGCAGATATTCCAGAGCTCTTGGATGTTCGTAAACACTTTAAATCAAAGTATGGAAAAGAATTTGTCTCGGCCGCAGTGGAGCTGCGCCCAGAATGTGGAGTGAATCGCATGTTAGTCGAAAAACTATCTGCTAAAGCACCGGATGGGCAGACCAAACTTAAAATCTTGACTGCAATTGCTGAGGAGTATAACATCAAATGGGACCCAAAATCCTTTGGTGACAGCATCAACCCTCCGGCTGATTTGTTGGTAATTTTAGTTTTTATAAAAAAGAACTATTTCCTTCAATGCTCAAGGTGTTTTTATCAGGAAATGAAGACTTACCTGAATTATTTTATTTTAATATTGAATTTCGGTTTTAGTAATACTAGTTATGAACTTTGTTGA

Protein sequence

MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVREEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPKSFGDSINPPADLLVILVFIKKNYFLQCSRCFYQEMKTYLNYFILILNFGFSNTSYELC

Homology

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.1e-26
Identity = 69/190 (36.32%), Postives = 113/190 (59.47%), Query Frame = 0


Query: 5   SFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVREEK 64
           S+   K K  LKLAVSRI++L+NKK   ++  K ++A+LL    +++ARIRVE  +R+E 
Sbjct: 7   SYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEY 66

Query: 65  SKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKHFK 124
             E +++IE+ CEL+ AR+ +I +    P+++KE++ +++++S R   IPEL  ++   K
Sbjct: 67  LIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQR-IQIPELEQIKNQLK 126

Query: 125 SKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPKSFGD 184
           +KYGK   + A        VN  +V KLS   PD     + L+ IAE++N+ W     G 
Sbjct: 127 AKYGKGLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW----CGS 186

Query: 185 SINPPADLLV 195
              PP  L++
Sbjct: 187 DYPPPPQLIM 190

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 69/190 (36.32%), Postives = 112/190 (58.95%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KSFGDSINPP 190
            S   +  PP
Sbjct: 181 DSVVMAEAPP 189

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.7e-25
Identity = 67/182 (36.81%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KS 182
            S
Sbjct: 181 DS 181

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.7e-25
Identity = 67/182 (36.81%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KS 182
            S
Sbjct: 181 DS 181

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.1e-25
Identity = 68/190 (35.79%), Postives = 111/190 (58.42%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML    K  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KSFGDSINPP 190
            S   +  PP
Sbjct: 181 DSVVMAEAPP 189

[Close]

BLAST of Cucsat.G7523.T5 vs. NCBI nr
Match: KAE8653413.1 (hypothetical protein Csa_007078 [Cucumis sativus])

HSP 1 Score: 374 bits (959), Expect = 8.14e-129
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. NCBI nr
Match: XP_031745744.1 (IST1-like protein [Cucumis sativus] >KAE8637468.1 hypothetical protein CSA_004501 [Cucumis sativus])

HSP 1 Score: 374 bits (959), Expect = 1.56e-124
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. NCBI nr
Match: XP_031742590.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101217093 [Cucumis sativus])

HSP 1 Score: 374 bits (959), Expect = 1.41e-118
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. NCBI nr
Match: XP_008450820.1 (PREDICTED: uncharacterized protein LOC103492291 [Cucumis melo] >TYK10119.1 Ist1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 371 bits (952), Expect = 3.70e-117
Identity = 191/193 (98.96%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML+KSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLNKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+KLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. NCBI nr
Match: XP_038879144.1 (uncharacterized protein LOC120071136 [Benincasa hispida])

HSP 1 Score: 363 bits (931), Expect = 4.81e-114
Identity = 186/193 (96.37%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDV IKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVQIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPEL+DVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELMDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+K+KILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKIKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGD+INPPAD L
Sbjct: 181 SFGDNINPPADFL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL
Match: A0A0A0LWR1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G580230 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 1.92e-118
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL
Match: A0A5D3CIP5 (Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002620 PE=3 SV=1)

HSP 1 Score: 371 bits (952), Expect = 1.79e-117
Identity = 191/193 (98.96%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML+KSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLNKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+KLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL
Match: A0A1S3BQ24 (uncharacterized protein LOC103492291 OS=Cucumis melo OX=3656 GN=LOC103492291 PE=3 SV=1)

HSP 1 Score: 371 bits (952), Expect = 1.79e-117
Identity = 191/193 (98.96%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML+KSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLNKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+KLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGDSINPPADLL
Sbjct: 181 SFGDSINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL
Match: A0A6J1EJ03 (uncharacterized protein LOC111434877 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434877 PE=3 SV=1)

HSP 1 Score: 359 bits (922), Expect = 3.79e-113
Identity = 182/193 (94.30%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDVH+KQLKG+LAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVHVKQLKGELAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKE+VSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKESVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFK+KYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDG +K+KILT IAEEYN+KWDPK
Sbjct: 121 KHFKAKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGPSKIKILTKIAEEYNVKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGD+INPPADLL
Sbjct: 181 SFGDNINPPADLL 193

[Close]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL
Match: A0A6J1KTU3 (uncharacterized protein LOC111496330 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496330 PE=3 SV=1)

HSP 1 Score: 359 bits (922), Expect = 4.70e-113
Identity = 182/193 (94.30%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDVH+KQLKG+LAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVHVKQLKGELAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKE+VSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKESVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFK+KYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDG +K+KILT IAEEYN+KWDPK
Sbjct: 121 KHFKAKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGPSKIKILTKIAEEYNVKWDPK 180

Query: 181 SFGDSINPPADLL 193
           SFGD+INPPADLL
Sbjct: 181 SFGDNINPPADLL 193

The following BLAST results are available for this feature:

BLAST of Cucsat.G7523.T5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54I39	1.1e-26	36.32	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]
P53990	1.2e-25	36.32	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
Q9CX00	4.7e-25	36.81	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
Q568Z6	4.7e-25	36.81	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]
Q3ZBV1	6.1e-25	35.79	IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1	[more]

BLAST of Cucsat.G7523.T5 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8653413.1	8.14e-129	100.00	hypothetical protein Csa_007078 [Cucumis sativus]	[more]
XP_031745744.1	1.56e-124	100.00	IST1-like protein [Cucumis sativus] >KAE8637468.1 hypothetical protein CSA_00450...	[more]
XP_031742590.1	1.41e-118	100.00	LOW QUALITY PROTEIN: uncharacterized protein LOC101217093 [Cucumis sativus]	[more]
XP_008450820.1	3.70e-117	98.96	PREDICTED: uncharacterized protein LOC103492291 [Cucumis melo] >TYK10119.1 Ist1 ...	[more]
XP_038879144.1	4.81e-114	96.37	uncharacterized protein LOC120071136 [Benincasa hispida]	[more]

BLAST of Cucsat.G7523.T5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWR1	1.92e-118	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G580230 PE=3 SV=1	[more]
A0A5D3CIP5	1.79e-117	98.96	Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BQ24	1.79e-117	98.96	uncharacterized protein LOC103492291 OS=Cucumis melo OX=3656 GN=LOC103492291 PE=...	[more]
A0A6J1EJ03	3.79e-113	94.30	uncharacterized protein LOC111434877 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1KTU3	4.70e-113	94.30	uncharacterized protein LOC111496330 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 12..176 e-value: 7.2E-60 score: 201.6
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 3..193
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 1..185 e-value: 1.9E-76 score: 257.8
None	No IPR available	PANTHER	PTHR12161:SF13	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 3..193

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G7523	Cucsat.G7523	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7523.T5.E1	Cucsat.G7523.T5.E1	exon
Cucsat.G7523.T5.E2	Cucsat.G7523.T5.E2	exon
Cucsat.G7523.T5.E3	Cucsat.G7523.T5.E3	exon
Cucsat.G7523.T5.E4	Cucsat.G7523.T5.E4	exon
Cucsat.G7523.T5.E5	Cucsat.G7523.T5.E5	exon
Cucsat.G7523.T5.E6	Cucsat.G7523.T5.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7523.T5.C1	Cucsat.G7523.T5.C1	CDS
Cucsat.G7523.T5.C2	Cucsat.G7523.T5.C2	CDS
Cucsat.G7523.T5.C3	Cucsat.G7523.T5.C3	CDS
Cucsat.G7523.T5.C4	Cucsat.G7523.T5.C4	CDS
Cucsat.G7523.T5.C5	Cucsat.G7523.T5.C5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7523.T5	Cucsat.G7523.T5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport