Cucsat.G7222.T29 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G7222.T29
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Inactive protein kinase
Location	ctg1528: 3927316 .. 3928244 (-)
RNA-Seq Expression	Cucsat.G7222.T29
Synteny	Cucsat.G7222.T29

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGTTCTTAAATGTCTTCCTATTTCCTCGCCAATGACTTCAAATCTGTGAAGAGCTAGATCACTCTTTGAATTCCTCGGAATCAAATGCTCAAGAAAAGATTATCACCTAAACACGAGGATCTTTAGTTAAAATGAGATAGGAATTTGCAACTTTTTGAGAAAATTTTACAAAATGACTACCCCACTGATGATAATGTCAAAATATTTGGTTGACCAATTTATTACATTCAACTATTTAAGTTCACTACTATTGTGTCTGTTCTGTTCTTTCTGCCTTTCAATTAATCTTTTTCCCTCTGATGTAAACGTTTTGACGGGAAATTTTATCTTTACAGGTATGTGGGTTCGGGGGTTTTCTCGACTTACCAGTGATTGTGCAATCGGTCATTTGAGAACTCATTCAGGAACTTTTTCCGATCGGAAGGATGACATTGTGCATTCATGTTCTCAGATGGTGCATCAGCTTCATGGTGCATATGACTCACTGAAGGTCTGTTCAATAACTTGCTCCTAAGGGTGATTACGGCCTTTGATCTTTATTGTCTACAAATCCTTAGGTTTTGGGATTTTTTTTGGCAGATAAAAGTCAGAATCAAAGTTCTTTCAGGCTTGGTTCGGGGTATGGTGGCTACCGAAGCTAAGAAAGCTCAATCAAACTGGGTGATATTGGATAAGTAATAGATATAATGTAAAATTTGTCTCTATTATAGGCATCCATTCATATGAACTCTGTGCATTTAGGCATATGAGATGATTTCATATTACGTAGTTGCCGATGCATTTGGATTCTTCGTTTTATAACTTTAAAGCTTTACTTTCCCTTTTCTTTTGTCTCCCACTTCCTCACTCTTGGTTTTTCACAATTATGGTCAGGAATTTGAAAGATGAAAGGAAAAACTGTTTGGAAGAGCTGCAATGCAATGTTGTT

Coding sequence (CDS)

ATGACTACCCCACTGATGATAATGTCAAAATATTTGGTTGACCAATTTATTACATTCAACTATTTAAGTTCACTACTATTGTGTCTGTTCTGTTCTTTCTGCCTTTCAATTAATCTTTTTCCCTCTGATGTAAACGTTTTGACGGGAAATTTTATCTTTACAGGTATGTGGGTTCGGGGGTTTTCTCGACTTACCAGTGATTGTGCAATCGGTCATTTGAGAACTCATTCAGGAACTTTTTCCGATCGGAAGGATGACATTGTGCATTCATGTTCTCAGATGGTGCATCAGCTTCATGGTGCATATGACTCACTGAAGATAAAAGTCAGAATCAAAGTTCTTTCAGGCTTGGTTCGGGGTATGGTGGCTACCGAAGCTAAGAAAGCTCAATCAAACTGGGTGATATTGGATAAGAATTTGAAAGATGAAAGGAAAAACTGTTTGGAAGAGCTGCAATGCAATGTTGTT

Protein sequence

MTTPLMIMSKYLVDQFITFNYLSSLLLCLFCSFCLSINLFPSDVNVLTGNFIFTGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV

Homology

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy Swiss-Prot
Match: P0DH62 (Inactive protein kinase SELMODRAFT_444075 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_444075 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 6.4e-07
Identity = 24/63 (38.10%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 94  MVHQLHGAYDSLKIKVRIKVLSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQC 153
           ++ +L   +D  K+   +++L    RG++ +EAK+ ++ WV+LD+NLK E K CL+EL  
Sbjct: 2   VMEKLRKIHDLKKVHTTLEILQFARRGVIPSEAKRFRATWVVLDRNLKSEGKLCLQELNS 61

Query: 154 NVV 157
           N+V
Sbjct: 62  NIV 64

[Close]

BLAST of Cucsat.G7222.T29 vs. NCBI nr
Match: KAE8637474.1 (hypothetical protein CSA_004507 [Cucumis sativus])

HSP 1 Score: 207 bits (527), Expect = 2.62e-60
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 54  TGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113
           + MWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV
Sbjct: 54  SSMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113

Query: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. NCBI nr
Match: XP_004135703.2 (LOW QUALITY PROTEIN: inactive protein kinase SELMODRAFT_444075 [Cucumis sativus])

HSP 1 Score: 207 bits (527), Expect = 4.07e-60
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 54  TGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113
           + MWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV
Sbjct: 54  SSMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113

Query: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. NCBI nr
Match: XP_008450837.1 (PREDICTED: inactive protein kinase SELMODRAFT_444075 [Cucumis melo])

HSP 1 Score: 205 bits (521), Expect = 3.03e-59
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116
           WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG
Sbjct: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116

Query: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. NCBI nr
Match: KGN66603.2 (hypothetical protein Csa_007423 [Cucumis sativus])

HSP 1 Score: 207 bits (527), Expect = 6.69e-59
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 54  TGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113
           + MWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV
Sbjct: 20  SSMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 79

Query: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 80  LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 122

[Close]

BLAST of Cucsat.G7222.T29 vs. NCBI nr
Match: TYK10107.1 (inactive protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 205 bits (521), Expect = 7.09e-59
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116
           WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG
Sbjct: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116

Query: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL
Match: A0A0A0M1W5 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586830 PE=4 SV=1)

HSP 1 Score: 207 bits (527), Expect = 1.97e-60
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 54  TGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113
           + MWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV
Sbjct: 54  SSMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKV 113

Query: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 114 LSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL
Match: A0A1S3BQ62 (inactive protein kinase SELMODRAFT_444075 OS=Cucumis melo OX=3656 GN=LOC103492308 PE=4 SV=1)

HSP 1 Score: 205 bits (521), Expect = 1.47e-59
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116
           WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG
Sbjct: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116

Query: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL
Match: A0A5D3CDQ3 (Inactive protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002490 PE=4 SV=1)

HSP 1 Score: 205 bits (521), Expect = 3.43e-59
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116
           WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG
Sbjct: 57  WVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHGAYDSLKIKVRIKVLSG 116

Query: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV
Sbjct: 117 LVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL
Match: A0A6J1F1F0 (inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441236 PE=4 SV=1)

HSP 1 Score: 188 bits (477), Expect = 3.77e-53
Identity = 92/116 (79.31%), Postives = 99/116 (85.34%), Query Frame = 0


Query: 41  PSDVNVLTGNFIFTGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHG 100
           PS  ++L  N    G W+RGFSR TSDCAIGHLRT S T SD+KDDIVHSCSQMVHQLHG
Sbjct: 50  PSHHSILMENLTVIGKWLRGFSRFTSDCAIGHLRTPSETLSDQKDDIVHSCSQMVHQLHG 109

Query: 101 AYDSLKIKVRIKVLSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           AY+ LKIKVRIK LSGL RG+VATEAKK QSNWVILDK+LKDERKNCLEELQCNVV
Sbjct: 110 AYEPLKIKVRIKALSGLARGIVATEAKKVQSNWVILDKHLKDERKNCLEELQCNVV 165

[Close]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL
Match: A0A6J1KPL8 (inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497565 PE=4 SV=1)

HSP 1 Score: 186 bits (471), Expect = 2.74e-52
Identity = 91/116 (78.45%), Postives = 99/116 (85.34%), Query Frame = 0


Query: 41  PSDVNVLTGNFIFTGMWVRGFSRLTSDCAIGHLRTHSGTFSDRKDDIVHSCSQMVHQLHG 100
           PS  ++L  N    G W+RGFSR TSDCAIGHLRT S T SD+KDDIVHSCSQMVHQL+G
Sbjct: 50  PSHHSILMENLTVIGKWLRGFSRFTSDCAIGHLRTPSETLSDQKDDIVHSCSQMVHQLYG 109

Query: 101 AYDSLKIKVRIKVLSGLVRGMVATEAKKAQSNWVILDKNLKDERKNCLEELQCNVV 156
           AY+ LKIKVRIK LSGL RG+VATEAKK QSNWVILDK+LKDERKNCLEELQCNVV
Sbjct: 110 AYEPLKIKVRIKALSGLARGIVATEAKKVQSNWVILDKHLKDERKNCLEELQCNVV 165

The following BLAST results are available for this feature:

BLAST of Cucsat.G7222.T29 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0DH62	6.4e-07	38.10	Inactive protein kinase SELMODRAFT_444075 OS=Selaginella moellendorffii OX=88036...	[more]

BLAST of Cucsat.G7222.T29 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8637474.1	2.62e-60	98.06	hypothetical protein CSA_004507 [Cucumis sativus]	[more]
XP_004135703.2	4.07e-60	98.06	LOW QUALITY PROTEIN: inactive protein kinase SELMODRAFT_444075 [Cucumis sativus]	[more]
XP_008450837.1	3.03e-59	100.00	PREDICTED: inactive protein kinase SELMODRAFT_444075 [Cucumis melo]	[more]
KGN66603.2	6.69e-59	98.06	hypothetical protein Csa_007423 [Cucumis sativus]	[more]
TYK10107.1	7.09e-59	100.00	inactive protein kinase [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G7222.T29 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M1W5	1.97e-60	98.06	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586...	[more]
A0A1S3BQ62	1.47e-59	100.00	inactive protein kinase SELMODRAFT_444075 OS=Cucumis melo OX=3656 GN=LOC10349230...	[more]
A0A5D3CDQ3	3.43e-59	100.00	Inactive protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1F1F0	3.77e-53	79.31	inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita moschata ...	[more]
A0A6J1KPL8	2.74e-52	78.45	inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita maxima OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR27001	OS01G0253100 PROTEIN	coord: 55..156
None	No IPR available	PANTHER	PTHR27001:SF866	SUBFAMILY NOT NAMED	coord: 55..156

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G7222	Cucsat.G7222	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7222.T29.E1	Cucsat.G7222.T29.E1	exon
Cucsat.G7222.T29.E2	Cucsat.G7222.T29.E2	exon
Cucsat.G7222.T29.E3	Cucsat.G7222.T29.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7222.T29.C3	Cucsat.G7222.T29.C3	CDS
Cucsat.G7222.T29.C2	Cucsat.G7222.T29.C2	CDS
Cucsat.G7222.T29.C1	Cucsat.G7222.T29.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7222.T29	Cucsat.G7222.T29-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0006470	protein dephosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004722	protein serine/threonine phosphatase activity
molecular_function	GO:0004713	protein tyrosine kinase activity