Cucsat.G7222.T22 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G7222.T22
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionInactive protein kinase
Locationctg1528: 3924366 .. 3929496 (-)
RNA-Seq ExpressionCucsat.G7222.T22
SyntenyCucsat.G7222.T22
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTGTTGGATTCATGAATAAGTTTTGATTACGTATTCATTTAATTAAGCCAATCTCTCCATTTCTTAGTGTCGTTTTGTTTGTTCCAACTCCAAACATTTGGCAATTTTGTTCTGTAATTGTGTCTTAATCTTGTAGACTGTAGAGAGAATATGCTTCTTACAATGCCAAACCAAAAGGCATTGAAAAACAGTGCGTTCAACCAAATGGGTCGAAAATTAGCTCAATTTTACTTGCTAAAGTTTGTGGGGCAGCGTGAATTTCACATGTAAAAAAGGAGGGTTCGCTCGCTCGCACTCACTCTACTTTCTTCTTTAATCTTCCTTTTTCTTCCTCATTTCACAAGGTGAGGCAAAAGCACCTCGGTTTCCGAGACATTTCCATCGCTGATTTCACTGCTCATTGCTGCAATATTGTGATTACAGTGTACCCTTTTGTTTCTTTTTTCCTGTATTCAGTGTCGGTTTCTTTTTGGGGGTTTTCATTTCATGATGGGGTCTCTTTTTAATGGAGATTTCTGACATGGTTATGGAAATTTGATCCTCGAGGTTGGGAACTGAACTGGGTTTGTGGATATTGTGAGATTTTTTTTGAAGAGTTTATGGAAATTTCTTACATGGTTTTGTGGATATTGTGAGAGTTTCTGAAGGATTTACGGAATTTTGATCCTTAAGGTTGAGAACTGAACTGGGTTTGTGGATATTGTGAGAGTTTCTGAAGGGTTTACGGAGTTTGATCCTTAAGGTTGGGAACTGGACTGGGTTTGTGGATATTGTGAGATTTTTTTAAGAGTTTATGGAGATTTCTTACATGGGTTTGGAAGTTTGATCCTTAAGGTTGGGAACTGAACTGGGTTTGTTGTTATTGTGAGAGCTTTTGAAGGTTTTATGGAGGAAGGACATCCAGACGTGGCTGGGAAAGTGGTGGTTGTCGCAATTAAAGCCACCTCGAAGGAAGTTTCTAAAGCTGCTCTCGTTTGGGCTTTGACGCATGTTGTTCAACCAGGAGATCATATTAAGCTCCTTGTGGTTATCCCTTCTCATCAATCAAGTAAGATTCATTTACTTTTATGCGACTTGTTAACGTCAAACTGGATTAAATTCTTTGTGTAAGTTCTTGGTCCATTATGATGGAAAAGAGTATATAAGTTTTCATACACGTTTTGATTGCATTTATGATGTCCAGATGAGATGCTTGTGAAATAAATGTTCGTCTCAGCATTTATTTTGAGTAATAGTAATTGTTTCTTTTGGGGTTCTTAAATGTCTTCCTATTTCCTCGCCAATGACTTCAAATCTGTGAAGAGCTAGATCACTCTTTGAATTCCTCGGAATCAAATGCTCAAGAAAAGATTATCACCTAAACACGAGGATCTTTAGTTAAAATGAGATAGGAATTTGCAACTTTTTGAGAAAATTTTACAAAATGACTACCCCACTGATGATAATGTCAAAATATTTGGTTGACCAATTTATTACATTCAACTATTTAAGTTCACTACTATTGTGTCTGTTCTGTTCTTTCTGCCTTTCAATTAATCTTTTTCCCTCTGATGTAAACGTTTTGACGGGAAATTTTATCTTTACAGGTATGTGGGTTCGGGGGTTTTCTCGACTTACCAGTGATTGTGCAATCGGTCATTTGAGAACTCATTCAGGAACTTTTTCCGATCGGAAGGATGACATTGTGCATTCATGTTCTCAGATGGTGCATCAGCTTCATGGTGCATATGACTCACTGAAGGTCTGTTCAATAACTTGCTCCTAAGGGTGATTACGGCCTTTGATCTTTATTGTCTACAAATCCTTAGGTTTTGGGATTTTTTTTGGCAGATAAAAGTCAGAATCAAAGTTCTTTCAGGCTTGGTTCGGGGTATGGTGGCTACCGAAGCTAAGAAAGCTCAATCAAACTGGGTGATATTGGATAAGTAATAGATATAATGTAAAATTTGTCTCTATTATAGGCATCCATTCATATGAACTCTGTGCATTTAGGCATATGAGATGATTTCATATTACGTAGTTGCCGATGCATTTGGATTCTTCGTTTTATAACTTTAAAGCTTTACTTTCCCTTTTCTTTTGTCTCCCACTTCCTCACTCTTGGTTTTTCACAATTATGGTCAGGAATTTGAAAGATGAAAGGAAAAACTGTTTGGAAGAGCTGCAATGCAATGTTGTTCTTATGAAGAAATATCACCCTAAGGTTCTTCGTTTGAATTTGATGGAATCCCCTAAAATGAACACTAGAGAAGCTTGGATATCATCACATGAATTAGACGTTTCTCAGAAGTGCCTTAAAAGCTATTTTGATGAACCGATCATGTTTACAGCTCCTGATGTGACTCCTGACAGTACTCCTGACGTAGAGTCTCCTTTTACCGTCACTGATATTGGAACATCATCAATATCGAGCTCGGATGTGGGCAGTTCGTCCTTGTTCTCTGGGATTTGTGGAAGCTTGAGAAATGATTCCAGAACAGCTGTCGACAGAGGGAGAAATATGTCTGGATCTGAATATGATTCTGAAAGTGAAAAGCAAACTCCCTCAGTTTCATATTTCCAGCGTTGTATGGTTGATATTATGAGTTCCCGGCGTAAATTCCAGCAACATGCAATGGAAGAATCACAAAATGCTCATCATAGACCTCCAGCTCCAACACGTCAAGGTCTGGTAAAAAAAATGTCTACTCTTTCTGTAGAACCTAGCCATGATGTGGCACATCGGAGTACTGATATTAGCTCAAGCAGAAACATAAGGAACACGGTATCATTGTCCAGAAAAGCACCTCTAGGCCCTCCTCCGCTATGTTCTATGTGCCAACACAAGGCCCCTGCATTTGGGAATCCTCCTAGATGGTTCACTTATGCTGAACTTGAAGTTGCTACAAGTGGCTTTGCACAAACAAATTTTTTGGCTGAGGGTGGATTTGGGTCTGTGCACCGAGGCATCTTATCAGATGGACAAGTTGTTGCTGTCAAGCAATATAAACTGGCTAGTACACAGGGAGATCGAGAATTTTGTTCAGAAGTTGAGGTCTTAAGTTGTGCCCAACATCGAAACGTAGTGATGCTTATTGGATTTTGTGTGGAGGGTGGAAGAAGGTTACTTGTTTATGAATATATTTGCAATGGATCTCTTGATTCCCATCTTTATGGTAATACCCTATTTCAGAATATCTTCTGGTATGGTTGTAATGGAACAGCCTGTGATTCATTACTACACATAAAATCACATAGGGAGCCCAAATAATGTCAGTTTTAGCGTCTTTATTAGTAAATCATCTTTTCGAAGTTCATGGCGTCTTTGTTTACTTGATTGCTTCCCTCTTGTAGTAAATCATGTAGCTTCAGCTTTGGTTTGATGACCTATGCCCAGTCATATTAACTCTGCCATGTCCAATCTTTGCAGGAAGAAATCGTGAACCATTGCAATGGTCGGCACGCCAGAAAATTGCAGTCGGGGCTGCTCGAGGATTGAGATACCTTCACGAAGAATGTAGAGTGGGCTGTATTGTGCATCGTGATATCCGTCCAAACAATATCCTCCTTACTCATGATTTTGAGCCACTAGTGAGTCAATGCTCTCTCTTTTCATTAATCCTTAGCTGTGTTGTATGTGATTTGATGTAAGAGAATCAGAATTTTCATGGAGATTTATCAAGTTAAGCATTGATTATGAGGTTTTCAATTTCCGGCTTCCCAAAATTCTTACTTGTCAGTGTCACAGCCAAAAAGGAAATGGAGAAAATGCCATTTACTCTAAGGATGCATTCACATTTTTGTATTAACATAAGATTTTCTGTTCGTTTCCTTCCGAACAACCCATATTGATGCACAAACATTGACTAACTGTCAAATGAGAACATTTCGGAATTGTGTATTTTTGGGCTGATGTGAAAATTTGCTTTTGAGTCGTAGGTTGGAGATTTTGGGCTGGCAAGGTGGCAACCGGATGGTGACCTAGCAGTAGAAACCAGAATCCTTGGGAGATTTGGGTAACCATTTCAATCATGTTATTTCTTTCTTTTTCATTATACAAACTAGCAATCCAAAAATTAAATTGCAACTACTTGTGGTTGCTTAGCTACCTGGCTCCTGAGTATGCACAGAGTGGCCAAATCACAGAGAAAGCCGATACATACTCATTTGGCGTTGTCTTGCTGGAACTTGTGACTGGACGCAAAGCAATTGATTTAAATCGTCCCAAGGGCCAGCAATGCCTCACTGAATGGGTAAGTCAGTCTCAGAAGTGGCTAAGTTAGTAAAACCATAGGACTACAACTTACTTGCCTTACCTCAATGCCAAATTATTCAAGTTCCTTTCCTTTTCTGATTCAAATTCGTGATTTCCGATTGATTTTCTTTGCATTTCATGTTGGGATCAGGCACGAAACCTGTTGAGAAAAAATGCAATCTCTGAACTAGTTGATCCATGTTTGAGGAACTGTTATTCAGACGAGGAAGTTCACCGCATGCTGCAATGCGCTTCCTTGTGCATAAAGCGTGATCCGTATGTAAGACCGCGTATGTCTCAGGTAACTATATGATATTTCATTCCACAAGCACAAGCACAACCAAAACAAAACAAACACCGATACACAATCACTCTTAAAGCTAAACTTCAATTCATCCTTGAGTTGCAATGCATTCTCAAAGTGTTTTTTCTATCCTTTAACGACGCCTCGATGGCATTACACTTGTTGTAGGTGCTTCGGGTGTTGGAGGGCGACATCGTTCTGTAAAGCAGCAGCCATATAGGAGGCCATGTTGGAAAATTTTGCAGAGTTGTAACGGTATATATATGGTCTGGAATCAACACTAACATGGCTGAAGAGGGATGTTGGAGTAAAGACCAATTTGTCTTCTGAGAGGGTGATAAAAGGAATTCCACTTTGCAGAAAGCAGTTTTTCTTTTTTCTTTTTATTAATTTCATCATTTTAGTGATTATAGCTTTAGTTAATGGATTTGGTTGTAATGTAAATAAGGGGATCAAACTGTTCTTCTGTAACCATAAAAATGTCAAGATTTGGCTTGGGATAGTTCCAAATAAAGTTACTTGTATGATATTGTATGATATGTTAAATTAGGTTGAATATATATATGTCTTGGTCTTCATGGAAAGGTTTTC

Coding sequence (CDS)

ATGCATTTGGATTCTTCGTTTTATAACTTTAAAGCTTTACTTTCCCTTTTCTTTTGTCTCCCACTTCCTCACTCTTGGTTTTTCACAATTATGGTCAGGAATTTGAAAGATGAAAGGAAAAACTGTTTGGAAGAGCTGCAATGCAATGTTGTTCTTATGAAGAAATATCACCCTAAGGTTCTTCGTTTGAATTTGATGGAATCCCCTAAAATGAACACTAGAGAAGCTTGGATATCATCACATGAATTAGACGTTTCTCAGAAGTGCCTTAAAAGCTATTTTGATGAACCGATCATGTTTACAGCTCCTGATGTGACTCCTGACAGTACTCCTGACGTAGAGTCTCCTTTTACCGTCACTGATATTGGAACATCATCAATATCGAGCTCGGATGTGGGCAGTTCGTCCTTGTTCTCTGGGATTTGTGGAAGCTTGAGAAATGATTCCAGAACAGCTGTCGACAGAGGGAGAAATATGTCTGGATCTGAATATGATTCTGAAAGTGAAAAGCAAACTCCCTCAGTTTCATATTTCCAGCGTTGTATGGTTGATATTATGAGTTCCCGGCGTAAATTCCAGCAACATGCAATGGAAGAATCACAAAATGCTCATCATAGACCTCCAGCTCCAACACGTCAAGGTCTGGTAAAAAAAATGTCTACTCTTTCTGTAGAACCTAGCCATGATGTGGCACATCGGAGTACTGATATTAGCTCAAGCAGAAACATAAGGAACACGGTATCATTGTCCAGAAAAGCACCTCTAGGCCCTCCTCCGCTATGTTCTATGTGCCAACACAAGGCCCCTGCATTTGGGAATCCTCCTAGATGGTTCACTTATGCTGAACTTGAAGTTGCTACAAGTGGCTTTGCACAAACAAATTTTTTGGCTGAGGGTGGATTTGGGTCTGTGCACCGAGGCATCTTATCAGATGGACAAGTTGTTGCTGTCAAGCAATATAAACTGGCTAGTACACAGGGAGATCGAGAATTTTGTTCAGAAGTTGAGGTCTTAAGTTGTGCCCAACATCGAAACGTAGTGATGCTTATTGGATTTTGTGTGGAGGGTGGAAGAAGGTTACTTGTTTATGAATATATTTGCAATGGATCTCTTGATTCCCATCTTTATGGTAATACCCTATTTCAGAATATCTTCTGGTATGGTTGTAATGGAACAGCCTGTGATTCATTACTACACATAAAATCACATAGGGAGCCCAAATAA

Protein sequence

MHLDSSFYNFKALLSLFFCLPLPHSWFFTIMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKCLKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDSRTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPAPTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAPAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDREFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYGNTLFQNIFWYGCNGTACDSLLHIKSHREPK
Homology
BLAST of Cucsat.G7222.T22 vs. ExPASy Swiss-Prot
Match: P0DH62 (Inactive protein kinase SELMODRAFT_444075 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_444075 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.9e-57
Identity = 144/355 (40.56%), Postives = 193/355 (54.37%), Query Frame = 0

Query: 28  FTIMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHEL---D 87
           + ++ RNLK E K CL+EL  N+V++ + +PK+LRLNL         E  I S  +    
Sbjct: 41  WVVLDRNLKSEGKLCLQELNSNIVVVHRSNPKILRLNLKRRDLPYDEEESIDSSSVLLNG 100

Query: 88  VSQKCLKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGS 147
           +S   +   FD+                            SS SSS+  S          
Sbjct: 101 LSLSVMPKGFDQLYW------------------------ESSTSSSEASSP--------- 160

Query: 148 LRNDSRTAVDRGRNMSGSE--YDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQN 207
              DSR        +S  E    +E+ ++ PS S       ++++S           S  
Sbjct: 161 ---DSRLVTAPKFELSVLEELLKNETRRKGPSPS-------EVLNS---------TTSSP 220

Query: 208 AHHRPPAPTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCS 267
           A H+P       L  K S    E +    + S  +    ++R  + L +++   PPPLCS
Sbjct: 221 ASHKPQV-LNDFLRMKESREYTEETDTQRNVSRPVDRVSSVRKQIHLRKQSSPQPPPLCS 280

Query: 268 MCQHKAPAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKL 327
           +CQHK P FG PPR FT+AEL++AT GF+  NFLAEGG+GSV+RG L DGQ VAVKQ+KL
Sbjct: 281 ICQHKTPVFGKPPRKFTFAELQLATGGFSDVNFLAEGGYGSVYRGRLPDGQAVAVKQHKL 340

Query: 328 ASTQGDREFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 378
           ASTQGD+EFC+EVEVLSCAQ RN+VMLIG+C E  +RLLVYE++CNGSLDSHLYG
Sbjct: 341 ASTQGDKEFCAEVEVLSCAQQRNLVMLIGYCAEDKKRLLVYEFVCNGSLDSHLYG 342

BLAST of Cucsat.G7222.T22 vs. ExPASy Swiss-Prot
Match: Q9SX31 (Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 GN=PERK9 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.4e-29
Identity = 72/163 (44.17%), Postives = 97/163 (59.51%), Query Frame = 0

Query: 215 LVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAPAFGNP 274
           L K+   LS     DV   S   S++R+      +   AP+G        Q ++   GN 
Sbjct: 303 LRKREKRLSAVSGGDVT-PSPMSSTARSDSAFFRMQSSAPVGASKRSGSYQSQSGGLGNS 362

Query: 275 PRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDREFCSE 334
              F+Y EL  AT+GF+Q N L EGGFG V++GIL DG+VVAVKQ K+   QGDREF +E
Sbjct: 363 KALFSYEELVKATNGFSQENLLGEGGFGCVYKGILPDGRVVAVKQLKIGGGQGDREFKAE 422

Query: 335 VEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 378
           VE LS   HR++V ++G C+ G RRLL+Y+Y+ N  L  HL+G
Sbjct: 423 VETLSRIHHRHLVSIVGHCISGDRRLLIYDYVSNNDLYFHLHG 464

BLAST of Cucsat.G7222.T22 vs. ExPASy Swiss-Prot
Match: Q8GX23 (Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 GN=PERK5 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.1e-29
Identity = 59/111 (53.15%), Postives = 82/111 (73.87%), Query Frame = 0

Query: 267 KAPAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQ 326
           +A   G+    FTY EL +AT GFAQ+N L +GGFG VH+G+L  G+ VAVK  KL S Q
Sbjct: 289 QAATLGHNQSTFTYDELSIATEGFAQSNLLGQGGFGYVHKGVLPSGKEVAVKSLKLGSGQ 348

Query: 327 GDREFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 378
           G+REF +EV+++S   HR++V L+G+C+ GG+RLLVYE+I N +L+ HL+G
Sbjct: 349 GEREFQAEVDIISRVHHRHLVSLVGYCISGGQRLLVYEFIPNNTLEFHLHG 399

BLAST of Cucsat.G7222.T22 vs. ExPASy Swiss-Prot
Match: Q9ZUE0 (Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702 GN=PERK12 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 6.6e-27
Identity = 57/109 (52.29%), Postives = 76/109 (69.72%), Query Frame = 0

Query: 272 GNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDREF 331
           G+    F+Y EL   T GFA+ N L EGGFG V++G L DG+VVAVKQ K  S QGDREF
Sbjct: 353 GSGQTHFSYEELAEITQGFARKNILGEGGFGCVYKGTLQDGKVVAVKQLKAGSGQGDREF 412

Query: 332 CSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYGNTL 381
            +EVE++S   HR++V L+G+C+    RLL+YEY+ N +L+ HL+G  L
Sbjct: 413 KAEVEIISRVHHRHLVSLVGYCISDQHRLLIYEYVSNQTLEHHLHGKGL 461

BLAST of Cucsat.G7222.T22 vs. ExPASy Swiss-Prot
Match: Q9LS95 (Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana OX=3702 GN=PERK6 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 6.6e-27
Identity = 59/126 (46.83%), Postives = 84/126 (66.67%), Query Frame = 0

Query: 254 PLGPPPLCSMCQHKAPAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQ 313
           P  PPP      H + A G     FTY EL  AT GF+Q+  L +GGFG VH+GIL +G+
Sbjct: 307 PSVPPP------HPSVALGFNKSTFTYDELAAATQGFSQSRLLGQGGFGYVHKGILPNGK 366

Query: 314 VVAVKQYKLASTQGDREFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDS 373
            +AVK  K  S QG+REF +EV+++S   HR +V L+G+C+ GG+R+LVYE++ N +L+ 
Sbjct: 367 EIAVKSLKAGSGQGEREFQAEVDIISRVHHRFLVSLVGYCIAGGQRMLVYEFLPNDTLEF 426

Query: 374 HLYGNT 380
           HL+G +
Sbjct: 427 HLHGKS 426

BLAST of Cucsat.G7222.T22 vs. NCBI nr
Match: XP_004135703.2 (LOW QUALITY PROTEIN: inactive protein kinase SELMODRAFT_444075 [Cucumis sativus])

HSP 1 Score: 684 bits (1765), Expect = 6.86e-242
Identity = 345/348 (99.14%), Postives = 347/348 (99.71%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. NCBI nr
Match: KGN66603.2 (hypothetical protein Csa_007423 [Cucumis sativus])

HSP 1 Score: 684 bits (1765), Expect = 6.06e-238
Identity = 345/348 (99.14%), Postives = 347/348 (99.71%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC
Sbjct: 101 ILDKNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 160

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 161 LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 220

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 221 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 280

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 281 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 340

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 341 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 400

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 401 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 448

BLAST of Cucsat.G7222.T22 vs. NCBI nr
Match: XP_008450837.1 (PREDICTED: inactive protein kinase SELMODRAFT_444075 [Cucumis melo])

HSP 1 Score: 666 bits (1718), Expect = 9.39e-235
Identity = 338/348 (97.13%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPKMN REAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKMNAREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDE IMF APDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDESIMFRAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVD GRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDGGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAH+STDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHQSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. NCBI nr
Match: TYK10107.1 (inactive protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 666 bits (1718), Expect = 7.75e-234
Identity = 338/348 (97.13%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPKMN REAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKMNAREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDE IMF APDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDESIMFRAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVD GRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDGGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAH+STDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHQSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. NCBI nr
Match: XP_038879086.1 (inactive protein kinase SELMODRAFT_444075-like [Benincasa hispida])

HSP 1 Score: 644 bits (1662), Expect = 2.97e-226
Identity = 325/348 (93.39%), Postives = 334/348 (95.98%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPKMNTREAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKMNTREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDE IMF APD+TPDSTPDVESP TVTDIG SSISSSDVGSSSLFSGICGSLRN+S
Sbjct: 195 LKSYFDESIMFRAPDMTPDSTPDVESPLTVTDIGASSISSSDVGSSSLFSGICGSLRNES 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTA D GRNMSGSEYDSESEKQTPSVSYFQRC+VDI+SSRRKFQQH MEESQNAHHRPPA
Sbjct: 255 RTAADGGRNMSGSEYDSESEKQTPSVSYFQRCLVDIISSRRKFQQHVMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PT QGL KKMS LS+EPS DVAHRSTDISSSRNIRNTV+LSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTSQGLAKKMSNLSLEPSTDVAHRSTDISSSRNIRNTVALSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRG+LSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGVLSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. ExPASy TrEMBL
Match: A0A0A0M1W5 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586830 PE=4 SV=1)

HSP 1 Score: 684 bits (1765), Expect = 3.32e-242
Identity = 345/348 (99.14%), Postives = 347/348 (99.71%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. ExPASy TrEMBL
Match: A0A1S3BQ62 (inactive protein kinase SELMODRAFT_444075 OS=Cucumis melo OX=3656 GN=LOC103492308 PE=4 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 4.55e-235
Identity = 338/348 (97.13%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPKMN REAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKMNAREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDE IMF APDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDESIMFRAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVD GRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDGGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAH+STDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHQSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. ExPASy TrEMBL
Match: A0A5D3CDQ3 (Inactive protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002490 PE=4 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 3.75e-234
Identity = 338/348 (97.13%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNTREAWISSHELDVSQKC 89
           I+ +NLKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPKMN REAWISSHELDVSQKC
Sbjct: 135 ILDKNLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKMNAREAWISSHELDVSQKC 194

Query: 90  LKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 149
           LKSYFDE IMF APDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS
Sbjct: 195 LKSYFDESIMFRAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRNDS 254

Query: 150 RTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 209
           RTAVD GRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA
Sbjct: 255 RTAVDGGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPPA 314

Query: 210 PTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 269
           PTRQGLVKKMSTLSVEPSHDVAH+STDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP
Sbjct: 315 PTRQGLVKKMSTLSVEPSHDVAHQSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKAP 374

Query: 270 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 329
           AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR
Sbjct: 375 AFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGDR 434

Query: 330 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 482

BLAST of Cucsat.G7222.T22 vs. ExPASy TrEMBL
Match: A0A6J1KPM7 (inactive protein kinase SELMODRAFT_444075-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497565 PE=4 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 3.11e-211
Identity = 307/349 (87.97%), Postives = 325/349 (93.12%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNT-REAWISSHELDVSQK 89
           I+ ++LKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPK+NT REAWISSHELDVSQK
Sbjct: 135 ILDKHLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKINTTREAWISSHELDVSQK 194

Query: 90  CLKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRND 149
           CLKSYFDE   F  PD+TP STPDVESP T+TD+GTSSISSSDVGSSSLFSG CGSLRN+
Sbjct: 195 CLKSYFDESAAFRGPDMTPVSTPDVESPLTITDVGTSSISSSDVGSSSLFSGFCGSLRNE 254

Query: 150 SRTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPP 209
           SRTA + GRN+SGSE DSE+EKQTPSVSYFQRCMVDI+SSRRK QQHAMEESQN HHRPP
Sbjct: 255 SRTAAEGGRNLSGSECDSENEKQTPSVSYFQRCMVDIISSRRKIQQHAMEESQNVHHRPP 314

Query: 210 APTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA 269
           A TRQGLVKKMSTLS +P+ D   RST++SSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA
Sbjct: 315 ASTRQGLVKKMSTLSQDPNTDAVSRSTNVSSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA 374

Query: 270 PAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGD 329
           PAFGNPPRWFTYAELEVATSGFAQTNFLAEGG+GSVHRGIL DGQVVAVKQYKLASTQGD
Sbjct: 375 PAFGNPPRWFTYAELEVATSGFAQTNFLAEGGYGSVHRGILPDGQVVAVKQYKLASTQGD 434

Query: 330 REFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           +EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 435 QEFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 483

BLAST of Cucsat.G7222.T22 vs. ExPASy TrEMBL
Match: A0A6J1KPL8 (inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497565 PE=4 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 4.20e-211
Identity = 307/349 (87.97%), Postives = 325/349 (93.12%), Query Frame = 0

Query: 30  IMVRNLKDERKNCLEELQCNVVLMKKYHPKVLRLNLMESPKMNT-REAWISSHELDVSQK 89
           I+ ++LKDERKNCLEELQCNVVLMKK  PKVLRLNLMESPK+NT REAWISSHELDVSQK
Sbjct: 144 ILDKHLKDERKNCLEELQCNVVLMKKSQPKVLRLNLMESPKINTTREAWISSHELDVSQK 203

Query: 90  CLKSYFDEPIMFTAPDVTPDSTPDVESPFTVTDIGTSSISSSDVGSSSLFSGICGSLRND 149
           CLKSYFDE   F  PD+TP STPDVESP T+TD+GTSSISSSDVGSSSLFSG CGSLRN+
Sbjct: 204 CLKSYFDESAAFRGPDMTPVSTPDVESPLTITDVGTSSISSSDVGSSSLFSGFCGSLRNE 263

Query: 150 SRTAVDRGRNMSGSEYDSESEKQTPSVSYFQRCMVDIMSSRRKFQQHAMEESQNAHHRPP 209
           SRTA + GRN+SGSE DSE+EKQTPSVSYFQRCMVDI+SSRRK QQHAMEESQN HHRPP
Sbjct: 264 SRTAAEGGRNLSGSECDSENEKQTPSVSYFQRCMVDIISSRRKIQQHAMEESQNVHHRPP 323

Query: 210 APTRQGLVKKMSTLSVEPSHDVAHRSTDISSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA 269
           A TRQGLVKKMSTLS +P+ D   RST++SSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA
Sbjct: 324 ASTRQGLVKKMSTLSQDPNTDAVSRSTNVSSSRNIRNTVSLSRKAPLGPPPLCSMCQHKA 383

Query: 270 PAFGNPPRWFTYAELEVATSGFAQTNFLAEGGFGSVHRGILSDGQVVAVKQYKLASTQGD 329
           PAFGNPPRWFTYAELEVATSGFAQTNFLAEGG+GSVHRGIL DGQVVAVKQYKLASTQGD
Sbjct: 384 PAFGNPPRWFTYAELEVATSGFAQTNFLAEGGYGSVHRGILPDGQVVAVKQYKLASTQGD 443

Query: 330 REFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 377
           +EFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG
Sbjct: 444 QEFCSEVEVLSCAQHRNVVMLIGFCVEGGRRLLVYEYICNGSLDSHLYG 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0DH627.9e-5740.56Inactive protein kinase SELMODRAFT_444075 OS=Selaginella moellendorffii OX=88036... [more]
Q9SX311.4e-2944.17Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8GX233.1e-2953.15Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZUE06.6e-2752.29Proline-rich receptor-like protein kinase PERK12 OS=Arabidopsis thaliana OX=3702... [more]
Q9LS956.6e-2746.83Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana... [more]
Match NameE-valueIdentityDescription
XP_004135703.26.86e-24299.14LOW QUALITY PROTEIN: inactive protein kinase SELMODRAFT_444075 [Cucumis sativus][more]
KGN66603.26.06e-23899.14hypothetical protein Csa_007423 [Cucumis sativus][more]
XP_008450837.19.39e-23597.13PREDICTED: inactive protein kinase SELMODRAFT_444075 [Cucumis melo][more]
TYK10107.17.75e-23497.13inactive protein kinase [Cucumis melo var. makuwa][more]
XP_038879086.12.97e-22693.39inactive protein kinase SELMODRAFT_444075-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0M1W53.32e-24299.14Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586... [more]
A0A1S3BQ624.55e-23597.13inactive protein kinase SELMODRAFT_444075 OS=Cucumis melo OX=3656 GN=LOC10349230... [more]
A0A5D3CDQ33.75e-23497.13Inactive protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1KPM73.11e-21187.97inactive protein kinase SELMODRAFT_444075-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1KPL84.20e-21187.97inactive protein kinase SELMODRAFT_444075-like isoform X1 OS=Cucurbita maxima OX... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 294..378
e-value: 2.3E-14
score: 53.3
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 261..365
e-value: 2.2E-32
score: 113.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..171
NoneNo IPR availablePANTHERPTHR27001:SF866SUBFAMILY NOT NAMEDcoord: 29..378
NoneNo IPR availablePANTHERPTHR27001OS01G0253100 PROTEINcoord: 29..378
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 290..407
score: 12.358076
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 265..381

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G7222Cucsat.G7222gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7222.T22.E1Cucsat.G7222.T22.E1exon
Cucsat.G7222.T22.E2Cucsat.G7222.T22.E2exon
Cucsat.G7222.T22.E3Cucsat.G7222.T22.E3exon
Cucsat.G7222.T22.E4Cucsat.G7222.T22.E4exon
Cucsat.G7222.T22.E5Cucsat.G7222.T22.E5exon
Cucsat.G7222.T22.E6Cucsat.G7222.T22.E6exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7222.T22.C1Cucsat.G7222.T22.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G7222.T22Cucsat.G7222.T22-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0004713 protein tyrosine kinase activity