Cucsat.G6270.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G6270.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	LINE-1 retrotransposable element ORF2 protein
Location	ctg1425: 2567081 .. 2567905 (+)
RNA-Seq Expression	Cucsat.G6270.T1
Synteny	Cucsat.G6270.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTAACTTCTGATCTAACTAGAGTTTTACCCCTTCGAAATTGTGAACATCACCAATGGAATAAAATCAAGCAACATTTCAAATCCCCAAATGACAGTAGATGTATTGACCATCCCACTTGGAAATTGAACATTAATGGTAAATTCTCCGTTGCATCGGTTAAGAAAGTCTTTTCTAGCTTGAACCAAAGAGATGATTTTAATATTGATGATAACATTTTTTTCTCAACTTTGGAAAACCAAAATCTCCAAGAAATGTCATTTTTTCATATGGTCTCTTTTACATTGTTGTATTAACACTGCTGACATTCTTCAGAGAAAAGTTCCAACTCTCAATCTGAGTCCAAATCGGTGTTCTTTATGTAAAAGACACTCAGAGGAGATTGATCATATTTTCATAGATTGTCATACTGCCAAAATTCTATGGAACAAGCTTGAAGGACTTCTTGGATGGAAAAACCAACAGCAGAATGTTGTTTCCTTATGCTCTTTCCTCTGCTCAATAAAACAACGCAACAAAAGGGGTATCATCTTTTTTAATACTATTGCATCCGTGTTGTGGATCTTATGGTTGGAGAGAAATAATCGCATTTTCAAGGGCAAGGAAAAATCTACCATTGATCTCTGGGAAGACATCCTTTCTCTCTCAGGGCTGTGGGCTAGCAAATACTCTATGTTTGCAAATTGTAGTGCTTCCTCTATCGCTATCAATTTAAGTGTTTTCTAACTTTTTGTTGTGGGCTTAGCTCTAGCCCTTTAGCTTTTCACGCTGCCGTTTGGTTTTTGTCGTCTACTGTAATTGTACTAATTCTTCCTATATTAATG

Coding sequence (CDS)

ATGAGAAAAGTTCCAACTCTCAATCTGAGTCCAAATCGGTGTTCTTTATGTAAAAGACACTCAGAGGAGATTGATCATATTTTCATAGATTGTCATACTGCCAAAATTCTATGGAACAAGCTTGAAGGACTTCTTGGATGGAAAAACCAACAGCAGAATGTTGTTTCCTTATGCTCTTTCCTCTGCTCAATAAAACAACGCAACAAAAGGGGTATCATCTTTTTTAATACTATTGCATCCGTGTTGTGGATCTTATGGTTGGAGAGAAATAATCGCATTTTCAAGGGCAAGGAAAAATCTACCATTGATCTCTGGGAAGACATCCTTTCTCTCTCAGGGCTGTGGGCTAGCAAATACTCTATGTTTGCAAATTGTAGTGCTTCCTCTATCGCTATCAATTTAAGTGTTTTCTAA

Protein sequence

MRKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFLCSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSMFANCSASSIAINLSVF

Homology

[Close]

BLAST of Cucsat.G6270.T1 vs. NCBI nr
Match: TYK13741.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 137 bits (344), Expect = 4.34e-37
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 1   MRKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSF 60
           M+++P L   P+ C +CKR+ E+  H+FI C  AK +W  +   L       +   LC  
Sbjct: 110 MKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCIT 169

Query: 61  LCSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYS 120
           +CS KQ+ K+ +I FNT AS LW +WLERN RIF GKEK+  ++WEDI +L+GLW S+ S
Sbjct: 170 MCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKALAGLWTSRSS 229

Query: 121 MFANCSASSIAINLSVF 137
           +F+N  ASSIA+NL+ F
Sbjct: 230 LFSNYQASSIALNLNAF 246

[Close]

BLAST of Cucsat.G6270.T1 vs. NCBI nr
Match: TYK14764.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 136 bits (342), Expect = 8.68e-37
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 1   MRKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSF 60
           M+++P L   P+ C +CKR+ E+  H+FI C  AK +W  +   L       +   LC  
Sbjct: 110 MKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRTISSHLNSNVNCLSPKELCIT 169

Query: 61  LCSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYS 120
           +CS KQ+ K+ +I FNT AS LW +WLERN RIF GKEK+  ++WEDI +L+GLW S+ S
Sbjct: 170 MCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKALAGLWTSRSS 229

Query: 121 MFANCSASSIAINLSVF 137
           +F+N  ASSIA+NL+ F
Sbjct: 230 LFSNYQASSIALNLNAF 246

[Close]

BLAST of Cucsat.G6270.T1 vs. NCBI nr
Match: KAA0039950.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24553.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (362), Expect = 1.08e-36
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2    RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
            +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 1008 KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAKALLNWNSTPTDVQSLIQNI 1067

Query: 62   CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
            CS+  RN++G+I FNT A++LW +WLERNNRIFK +EK+  DLWED L+  GLW+ K  +
Sbjct: 1068 CSLNIRNQKGLITFNTNATILWKIWLERNNRIFKQQEKAPQDLWEDTLAQIGLWSCKSKL 1127

Query: 122  FANCSASSIAINLSVF 137
            F+N    SIA+N+S F
Sbjct: 1128 FSNYDCCSIALNISAF 1143

[Close]

BLAST of Cucsat.G6270.T1 vs. NCBI nr
Match: KAA0044556.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 142 bits (358), Expect = 3.67e-36
Identity = 63/136 (46.32%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2   RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
           +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 806 KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNI 865

Query: 62  CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
           CS+  + ++G+I FNTIA +LW +WLERNNRIFK ++K   DLWEDIL+ +GLW+ K  +
Sbjct: 866 CSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQQKKEFQDLWEDILAQTGLWSCKSKL 925

Query: 122 FANCSASSIAINLSVF 137
           F+N    SIA+N+S F
Sbjct: 926 FSNYDCCSIALNISAF 941

[Close]

BLAST of Cucsat.G6270.T1 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 142 bits (358), Expect = 3.72e-36
Identity = 63/136 (46.32%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2    RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
            +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 900  KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNI 959

Query: 62   CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
            CS+  + ++G+I FNTIA +LW +WLERNNRIFK ++K   DLWEDIL+ +GLW+ K  +
Sbjct: 960  CSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQQKKEFQDLWEDILAQTGLWSCKSKL 1019

Query: 122  FANCSASSIAINLSVF 137
            F+N    SIA+N+S F
Sbjct: 1020 FSNYDCCSIALNISAF 1035

[Close]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL
Match: A0A5D3CPL6 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold34842G00010 PE=4 SV=1)

HSP 1 Score: 137 bits (344), Expect = 2.10e-37
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 1   MRKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSF 60
           M+++P L   P+ C +CKR+ E+  H+FI C  AK +W  +   L       +   LC  
Sbjct: 110 MKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRSISSHLSSNVNCLSPKELCIT 169

Query: 61  LCSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYS 120
           +CS KQ+ K+ +I FNT AS LW +WLERN RIF GKEK+  ++WEDI +L+GLW S+ S
Sbjct: 170 MCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKALAGLWTSRSS 229

Query: 121 MFANCSASSIAINLSVF 137
           +F+N  ASSIA+NL+ F
Sbjct: 230 LFSNYQASSIALNLNAF 246

[Close]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL
Match: A0A5D3CUY9 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46132G00010 PE=4 SV=1)

HSP 1 Score: 136 bits (342), Expect = 4.20e-37
Identity = 64/137 (46.72%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 1   MRKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSF 60
           M+++P L   P+ C +CKR+ E+  H+FI C  AK +W  +   L       +   LC  
Sbjct: 110 MKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRTISSHLNSNVNCLSPKELCIT 169

Query: 61  LCSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYS 120
           +CS KQ+ K+ +I FNT AS LW +WLERN RIF GKEK+  ++WEDI +L+GLW S+ S
Sbjct: 170 MCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKALAGLWTSRSS 229

Query: 121 MFANCSASSIAINLSVF 137
           +F+N  ASSIA+NL+ F
Sbjct: 230 LFSNYQASSIALNLNAF 246

[Close]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 5.25e-37
Identity = 64/136 (47.06%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2    RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
            +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 1008 KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAKALLNWNSTPTDVQSLIQNI 1067

Query: 62   CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
            CS+  RN++G+I FNT A++LW +WLERNNRIFK +EK+  DLWED L+  GLW+ K  +
Sbjct: 1068 CSLNIRNQKGLITFNTNATILWKIWLERNNRIFKQQEKAPQDLWEDTLAQIGLWSCKSKL 1127

Query: 122  FANCSASSIAINLSVF 137
            F+N    SIA+N+S F
Sbjct: 1128 FSNYDCCSIALNISAF 1143

[Close]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL
Match: A0A5A7TR15 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003050 PE=4 SV=1)

HSP 1 Score: 142 bits (358), Expect = 1.78e-36
Identity = 63/136 (46.32%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2   RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
           +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 806 KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNI 865

Query: 62  CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
           CS+  + ++G+I FNTIA +LW +WLERNNRIFK ++K   DLWEDIL+ +GLW+ K  +
Sbjct: 866 CSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQQKKEFQDLWEDILAQTGLWSCKSKL 925

Query: 122 FANCSASSIAINLSVF 137
           F+N    SIA+N+S F
Sbjct: 926 FSNYDCCSIALNISAF 941

[Close]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL
Match: A0A5A7TIB8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00430 PE=4 SV=1)

HSP 1 Score: 142 bits (358), Expect = 1.80e-36
Identity = 63/136 (46.32%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 2    RKVPTLNLSPNRCSLCKRHSEEIDHIFIDCHTAKILWNKLEGLLGWKNQQQNVVSLCSFL 61
            +++P   LSPN C +C +  E+I+H+FI C  ++ LW+K + LL W +   +V SL   +
Sbjct: 900  KRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQLWSKAQALLKWNSTPNDVKSLAQNI 959

Query: 62   CSIKQRNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSM 121
            CS+  + ++G+I FNTIA +LW +WLERNNRIFK ++K   DLWEDIL+ +GLW+ K  +
Sbjct: 960  CSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQQKKEFQDLWEDILAQTGLWSCKSKL 1019

Query: 122  FANCSASSIAINLSVF 137
            F+N    SIA+N+S F
Sbjct: 1020 FSNYDCCSIALNISAF 1035

[Close]

BLAST of Cucsat.G6270.T1 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.6e-04
Identity = 32/130 (24.62%), Postives = 54/130 (41.54%), Query Frame = 0


Query: 14  CSLCKRHSEEIDHIFIDCHTAKILW------NKLEGLLGWKNQ-QQNVVSLCSFLCSIKQ 73
           C  C    E ++H+   C  A+++W         EG   W +    N+  + +    I +
Sbjct: 12  CVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPEG--EWTDSLYANLYWVLNLEVEIPK 71

Query: 74  RNKRGIIFFNTIASVLWILWLERNNRIFKGKEKSTIDLWEDILSLSGLWASKYSMFANCS 133
             K G    N +  +LW LW  RN  +FKGKE    ++    +     W+++  +    S
Sbjct: 72  LGKIG----NLVPWLLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKAS 131

Query: 134 ASSIAINLSV 137
              +  NLSV
Sbjct: 132 GPQVERNLSV 135

The following BLAST results are available for this feature:

BLAST of Cucsat.G6270.T1 vs.


Match Name	E-value	Identity	Description

BLAST of Cucsat.G6270.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK13741.1	4.34e-37	46.72	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
TYK14764.1	8.68e-37	46.72	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
KAA0039950.1	1.08e-36	47.06	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK245...	[more]
KAA0044556.1	3.67e-36	46.32	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
KAA0041397.1	3.72e-36	46.32	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G6270.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CPL6	2.10e-37	46.72	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3CUY9	4.20e-37	46.72	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7T9I7	5.25e-37	47.06	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7TR15	1.78e-36	46.32	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7TIB8	1.80e-36	46.32	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Cucsat.G6270.T1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	1.6e-04	24.62	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 11..38 e-value: 1.8E-6 score: 28.5

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G6270	Cucsat.G6270	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6270.T1.E1	Cucsat.G6270.T1.E1	exon
Cucsat.G6270.T1.E2	Cucsat.G6270.T1.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6270.T1.C1	Cucsat.G6270.T1.C1	CDS
Cucsat.G6270.T1.C2	Cucsat.G6270.T1.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6270.T1	Cucsat.G6270.T1-protein	polypeptide